Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069575.t1 | XP_013447177.1 | 92.8 | 181 | 13 | 0 | 1 | 181 | 1 | 181 | 2.50E-86 | 328.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069575.t1 | A0A072TUK3 | 92.8 | 181 | 13 | 0 | 1 | 181 | 1 | 181 | 1.8e-86 | 328.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057148 | MS.gene069575 | 0.811403 | 7.13E-51 | -1.69E-46 |
| MS.gene057986 | MS.gene069575 | 0.835592 | 1.53E-56 | -1.69E-46 |
| MS.gene059026 | MS.gene069575 | 0.804113 | 2.52E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069575.t1 | MTR_8g099345 | 92.818 | 181 | 13 | 0 | 1 | 181 | 1 | 181 | 1.08e-121 | 341 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069575.t1 | AT1G64355 | 75.556 | 135 | 26 | 3 | 32 | 162 | 43 | 174 | 6.24e-66 | 201 |
| MS.gene069575.t1 | AT1G64355 | 66.990 | 103 | 25 | 4 | 32 | 128 | 43 | 142 | 7.54e-37 | 125 |
| MS.gene069575.t1 | AT1G64355 | 63.158 | 95 | 28 | 3 | 32 | 122 | 43 | 134 | 1.54e-31 | 111 |
Find 44 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAGTTCCAGCTGGAATTTA+TGG | 0.258898 | 4.4:-4980538 | MS.gene069575:intron |
| GGTGAGGTTAGGAGAAGTTT+TGG | 0.272109 | 4.4:+4980694 | None:intergenic |
| GTTGAAGGGTTGATAAGTTT+AGG | 0.306773 | 4.4:+4980923 | None:intergenic |
| CACTAACTTGTTCATTGTGT+TGG | 0.314311 | 4.4:-4980172 | MS.gene069575:CDS |
| TTGAAGGGTTGATAAGTTTA+GGG | 0.316441 | 4.4:+4980924 | None:intergenic |
| TTAAATGAAAATCACGTTGC+AGG | 0.357937 | 4.4:+4980894 | None:intergenic |
| ACTAACTTGTTCATTGTGTT+GGG | 0.374119 | 4.4:-4980171 | MS.gene069575:CDS |
| TTGACAGCTAAGGTGCATTA+TGG | 0.386555 | 4.4:-4980252 | MS.gene069575:intron |
| TGCCCCTGGGTGTTGATCCA+TGG | 0.395407 | 4.4:-4980808 | MS.gene069575:CDS |
| AATCAGAAAAGCTGGAAATT+CGG | 0.416724 | 4.4:-4980136 | MS.gene069575:CDS |
| TCTCCTAACCTCACCCTCTT+CGG | 0.418605 | 4.4:-4980686 | MS.gene069575:CDS |
| AAACCGAAGAGGGTGAGGTT+AGG | 0.440423 | 4.4:+4980683 | None:intergenic |
| GATCGTGTGTGGATTGCCCC+TGG | 0.440978 | 4.4:-4980822 | MS.gene069575:CDS |
| TCTAATGTGGATTGGTTACA+TGG | 0.443829 | 4.4:-4980219 | MS.gene069575:CDS |
| CTTCTTTGTCTAATGTGGAT+TGG | 0.447286 | 4.4:-4980227 | MS.gene069575:CDS |
| CGTTGCAGGAAGTTCGTTGA+AGG | 0.448415 | 4.4:+4980908 | None:intergenic |
| ATGTGGATTGGTTACATGGT+GGG | 0.450371 | 4.4:-4980215 | MS.gene069575:CDS |
| TCGAAGGAGGAGAAGAAACC+TGG | 0.458784 | 4.4:-4980099 | MS.gene069575:CDS |
| TGGAACTTCTTTGTCTAATG+TGG | 0.464776 | 4.4:-4980232 | MS.gene069575:CDS |
| AGAGAGGGAATCAGAAAAGC+TGG | 0.466045 | 4.4:-4980144 | MS.gene069575:CDS |
| GCCCCTGGGTGTTGATCCAT+GGG | 0.473465 | 4.4:-4980807 | MS.gene069575:CDS |
| ATTGTGTTGGGTTTGAGAGA+GGG | 0.496509 | 4.4:-4980159 | MS.gene069575:CDS |
| AGCGATGCTCTGTGAATCAA+TGG | 0.506212 | 4.4:+4980778 | None:intergenic |
| GGAGAAGAAACCTGGGATGA+AGG | 0.519084 | 4.4:-4980091 | MS.gene069575:CDS |
| GACTCACCATAAATTCCAGC+TGG | 0.525068 | 4.4:+4980532 | None:intergenic |
| CATTGTGTTGGGTTTGAGAG+AGG | 0.529587 | 4.4:-4980160 | MS.gene069575:CDS |
| GTGCCCATGGATCAACACCC+AGG | 0.532974 | 4.4:+4980804 | None:intergenic |
| AAGTACAAGAAACCAATGTA+AGG | 0.534146 | 4.4:+4980728 | None:intergenic |
| CAAGTTAGTGAGAGTGAGAA+GGG | 0.535305 | 4.4:+4980185 | None:intergenic |
| TGTGGATTGGTTACATGGTG+GGG | 0.539691 | 4.4:-4980214 | MS.gene069575:CDS |
| AGTAGAAACCGAAGAGGGTG+AGG | 0.547358 | 4.4:+4980678 | None:intergenic |
| TGCCCATGGATCAACACCCA+GGG | 0.560771 | 4.4:+4980805 | None:intergenic |
| AGCAACTAGACCTTCATCCC+AGG | 0.562545 | 4.4:+4980081 | None:intergenic |
| GTTGCAGGAAGTTCGTTGAA+GGG | 0.573541 | 4.4:+4980909 | None:intergenic |
| ATCGTGTGTGGATTGCCCCT+GGG | 0.578639 | 4.4:-4980821 | MS.gene069575:CDS |
| AATGTGGATTGGTTACATGG+TGG | 0.588190 | 4.4:-4980216 | MS.gene069575:CDS |
| GATTTACAGAGAGATACAAC+AGG | 0.591539 | 4.4:+4980947 | None:intergenic |
| ACAAGTTAGTGAGAGTGAGA+AGG | 0.592216 | 4.4:+4980184 | None:intergenic |
| TGAAGGGTTGATAAGTTTAG+GGG | 0.614651 | 4.4:+4980925 | None:intergenic |
| CGAAGGAGGAGAAGAAACCT+GGG | 0.616213 | 4.4:-4980098 | MS.gene069575:CDS |
| AAGAGAACAGTGATCGTGTG+TGG | 0.618841 | 4.4:-4980833 | MS.gene069575:CDS |
| GAATCAATGGAAGGTGCCCA+TGG | 0.621299 | 4.4:+4980791 | None:intergenic |
| GCCCATGGATCAACACCCAG+GGG | 0.698475 | 4.4:+4980806 | None:intergenic |
| ATGCTCTGTGAATCAATGGA+AGG | 0.701730 | 4.4:+4980782 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAAATAAATAAATAAAA+AGG | + | chr4.4:4980499-4980518 | None:intergenic | 0.0% |
| !! | TAATTTAATCATTTCAAATG+TGG | + | chr4.4:4980768-4980787 | None:intergenic | 15.0% |
| !!! | CTCTAATTGAACTTTATTTT+CGG | + | chr4.4:4980565-4980584 | None:intergenic | 20.0% |
| ! | AAGAAAAGAAAACTAACTTG+TGG | + | chr4.4:4980429-4980448 | None:intergenic | 25.0% |
| ! | ATTACTTATGTTTAGTTGTG+TGG | - | chr4.4:4980784-4980803 | MS.gene069575:CDS | 25.0% |
| ! | TTGATTTATTATGATTCAGC+TGG | - | chr4.4:4980697-4980716 | MS.gene069575:CDS | 25.0% |
| ! | TTTAATTGTTCTGTTTGGTT+CGG | - | chr4.4:4980720-4980739 | MS.gene069575:CDS | 25.0% |
| !!! | TTTTCTTTGTTTCCTTACAT+TGG | - | chr4.4:4980320-4980339 | MS.gene069575:intron | 25.0% |
| AAGTACAAGAAACCAATGTA+AGG | + | chr4.4:4980335-4980354 | None:intergenic | 30.0% | |
| AATCAGAAAAGCTGGAAATT+CGG | - | chr4.4:4980924-4980943 | MS.gene069575:CDS | 30.0% | |
| CTACGATTAAGTTATTCGAA+CGG | + | chr4.4:4980664-4980683 | None:intergenic | 30.0% | |
| TTAAATGAAAATCACGTTGC+AGG | + | chr4.4:4980169-4980188 | None:intergenic | 30.0% | |
| !! | ACTAACTTGTTCATTGTGTT+GGG | - | chr4.4:4980889-4980908 | MS.gene069575:CDS | 30.0% |
| !! | TTGAAGGGTTGATAAGTTTA+GGG | + | chr4.4:4980139-4980158 | None:intergenic | 30.0% |
| !!! | CAATGTTTTAAAAACCGAAC+TGG | - | chr4.4:4980593-4980612 | MS.gene069575:intron | 30.0% |
| !!! | GGTTCGGTTTTTAGAATATT+GGG | - | chr4.4:4980736-4980755 | MS.gene069575:CDS | 30.0% |
| !!! | TGGTTCGGTTTTTAGAATAT+TGG | - | chr4.4:4980735-4980754 | MS.gene069575:CDS | 30.0% |
| CAAAAAGTAGAAACCGAAGA+GGG | + | chr4.4:4980390-4980409 | None:intergenic | 35.0% | |
| CTTCTTTGTCTAATGTGGAT+TGG | - | chr4.4:4980833-4980852 | MS.gene069575:CDS | 35.0% | |
| GATTTACAGAGAGATACAAC+AGG | + | chr4.4:4980116-4980135 | None:intergenic | 35.0% | |
| TGGAAATTCGGAGAAAAACA+CGG | - | chr4.4:4980936-4980955 | MS.gene069575:CDS | 35.0% | |
| TGGAACTTCTTTGTCTAATG+TGG | - | chr4.4:4980828-4980847 | MS.gene069575:CDS | 35.0% | |
| ! | GCTGGTTTAATTGTTCTGTT+TGG | - | chr4.4:4980715-4980734 | MS.gene069575:CDS | 35.0% |
| !! | CACTAACTTGTTCATTGTGT+TGG | - | chr4.4:4980888-4980907 | MS.gene069575:CDS | 35.0% |
| !! | GTTGAAGGGTTGATAAGTTT+AGG | + | chr4.4:4980140-4980159 | None:intergenic | 35.0% |
| !! | TACTTTTTGCTCGCTTTTGT+TGG | - | chr4.4:4980401-4980420 | MS.gene069575:intron | 35.0% |
| !! | TCTAATGTGGATTGGTTACA+TGG | - | chr4.4:4980841-4980860 | MS.gene069575:CDS | 35.0% |
| !! | TGAAGGGTTGATAAGTTTAG+GGG | + | chr4.4:4980138-4980157 | None:intergenic | 35.0% |
| !!! | GTTAGGAGAAGTTTTGGATT+TGG | + | chr4.4:4980363-4980382 | None:intergenic | 35.0% |
| !!! | TTATTTTGAACAGTTCCAGC+TGG | - | chr4.4:4980513-4980532 | MS.gene069575:intron | 35.0% |
| AACCGAACTGGACATTAAAC+CGG | - | chr4.4:4980605-4980624 | MS.gene069575:intron | 40.0% | |
| ACAAGTTAGTGAGAGTGAGA+AGG | + | chr4.4:4980879-4980898 | None:intergenic | 40.0% | |
| ACAGCATACAGAATGACAGT+TGG | - | chr4.4:4980637-4980656 | MS.gene069575:intron | 40.0% | |
| ACAGTTCCAGCTGGAATTTA+TGG | - | chr4.4:4980522-4980541 | MS.gene069575:intron | 40.0% | |
| ATGCTCTGTGAATCAATGGA+AGG | + | chr4.4:4980281-4980300 | None:intergenic | 40.0% | |
| ATTGTGTTGGGTTTGAGAGA+GGG | - | chr4.4:4980901-4980920 | MS.gene069575:CDS | 40.0% | |
| CAAGTTAGTGAGAGTGAGAA+GGG | + | chr4.4:4980878-4980897 | None:intergenic | 40.0% | |
| GCAAAAAGTAGAAACCGAAG+AGG | + | chr4.4:4980391-4980410 | None:intergenic | 40.0% | |
| TTGACAGCTAAGGTGCATTA+TGG | - | chr4.4:4980808-4980827 | MS.gene069575:CDS | 40.0% | |
| !! | AATGTGGATTGGTTACATGG+TGG | - | chr4.4:4980844-4980863 | MS.gene069575:CDS | 40.0% |
| !! | ATGTGGATTGGTTACATGGT+GGG | - | chr4.4:4980845-4980864 | MS.gene069575:CDS | 40.0% |
| AACTGGACATTAAACCGGCA+TGG | - | chr4.4:4980610-4980629 | MS.gene069575:intron | 45.0% | |
| AAGAGAACAGTGATCGTGTG+TGG | - | chr4.4:4980227-4980246 | MS.gene069575:CDS | 45.0% | |
| AGAGAGGGAATCAGAAAAGC+TGG | - | chr4.4:4980916-4980935 | MS.gene069575:CDS | 45.0% | |
| AGCGATGCTCTGTGAATCAA+TGG | + | chr4.4:4980285-4980304 | None:intergenic | 45.0% | |
| CATTGTGTTGGGTTTGAGAG+AGG | - | chr4.4:4980900-4980919 | MS.gene069575:CDS | 45.0% | |
| GAAAAACACGGAATCGAAGG+AGG | - | chr4.4:4980948-4980967 | MS.gene069575:CDS | 45.0% | |
| GACTCACCATAAATTCCAGC+TGG | + | chr4.4:4980531-4980550 | None:intergenic | 45.0% | |
| GGAGAAAAACACGGAATCGA+AGG | - | chr4.4:4980945-4980964 | MS.gene069575:CDS | 45.0% | |
| GTTGCAGGAAGTTCGTTGAA+GGG | + | chr4.4:4980154-4980173 | None:intergenic | 45.0% | |
| TGTATGCTGTTGAACCATGC+CGG | + | chr4.4:4980627-4980646 | None:intergenic | 45.0% | |
| ! | GGTGAGGTTAGGAGAAGTTT+TGG | + | chr4.4:4980369-4980388 | None:intergenic | 45.0% |
| !! | TGCCGGTTTAATGTCCAGTT+CGG | + | chr4.4:4980610-4980629 | None:intergenic | 45.0% |
| !! | TGTGGATTGGTTACATGGTG+GGG | - | chr4.4:4980846-4980865 | MS.gene069575:CDS | 45.0% |
| AAACCGAAGAGGGTGAGGTT+AGG | + | chr4.4:4980380-4980399 | None:intergenic | 50.0% | |
| AGTAGAAACCGAAGAGGGTG+AGG | + | chr4.4:4980385-4980404 | None:intergenic | 50.0% | |
| CGAAGGAGGAGAAGAAACCT+GGG | - | chr4.4:4980962-4980981 | MS.gene069575:CDS | 50.0% | |
| CGTTGCAGGAAGTTCGTTGA+AGG | + | chr4.4:4980155-4980174 | None:intergenic | 50.0% | |
| GGAGAAGAAACCTGGGATGA+AGG | - | chr4.4:4980969-4980988 | MS.gene069575:CDS | 50.0% | |
| GTTGTGTGGCTTGACAGCTA+AGG | - | chr4.4:4980798-4980817 | MS.gene069575:CDS | 50.0% | |
| TCGAAGGAGGAGAAGAAACC+TGG | - | chr4.4:4980961-4980980 | MS.gene069575:CDS | 50.0% | |
| TCTCCTAACCTCACCCTCTT+CGG | - | chr4.4:4980374-4980393 | MS.gene069575:intron | 50.0% | |
| !! | GAATCAATGGAAGGTGCCCA+TGG | + | chr4.4:4980272-4980291 | None:intergenic | 50.0% |
| TGCCCATGGATCAACACCCA+GGG | + | chr4.4:4980258-4980277 | None:intergenic | 55.0% | |
| ! | ATCGTGTGTGGATTGCCCCT+GGG | - | chr4.4:4980239-4980258 | MS.gene069575:CDS | 55.0% |
| GCCCATGGATCAACACCCAG+GGG | + | chr4.4:4980257-4980276 | None:intergenic | 60.0% | |
| GTGCCCATGGATCAACACCC+AGG | + | chr4.4:4980259-4980278 | None:intergenic | 60.0% | |
| ! | GATCGTGTGTGGATTGCCCC+TGG | - | chr4.4:4980238-4980257 | MS.gene069575:CDS | 60.0% |
| !! | GCCCCTGGGTGTTGATCCAT+GGG | - | chr4.4:4980253-4980272 | MS.gene069575:intron | 60.0% |
| !! | TGCCCCTGGGTGTTGATCCA+TGG | - | chr4.4:4980252-4980271 | MS.gene069575:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 4980086 | 4980996 | 4980086 | ID=MS.gene069575 |
| chr4.4 | mRNA | 4980086 | 4980996 | 4980086 | ID=MS.gene069575.t1;Parent=MS.gene069575 |
| chr4.4 | exon | 4980651 | 4980996 | 4980651 | ID=MS.gene069575.t1.exon1;Parent=MS.gene069575.t1 |
| chr4.4 | CDS | 4980651 | 4980996 | 4980651 | ID=cds.MS.gene069575.t1;Parent=MS.gene069575.t1 |
| chr4.4 | exon | 4980539 | 4980556 | 4980539 | ID=MS.gene069575.t1.exon2;Parent=MS.gene069575.t1 |
| chr4.4 | CDS | 4980539 | 4980556 | 4980539 | ID=cds.MS.gene069575.t1;Parent=MS.gene069575.t1 |
| chr4.4 | exon | 4980086 | 4980267 | 4980086 | ID=MS.gene069575.t1.exon3;Parent=MS.gene069575.t1 |
| chr4.4 | CDS | 4980086 | 4980267 | 4980086 | ID=cds.MS.gene069575.t1;Parent=MS.gene069575.t1 |
| Gene Sequence |
| Protein sequence |
