AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057986


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057986.t1 MTR_3g023160 95.489 133 6 0 1 133 1 133 1.01e-75 221
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057986.t1 AT4G31560 46.897 145 55 7 1 132 1 136 1.05e-26 97.4

Find 42 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGCCGCAACAGGCCTAAA+AGG 0.219871 3.4:+14599938 MS.gene057986:CDS
CTTCACATTCCCTTCCTTGC+GGG 0.249184 3.4:+14598181 MS.gene057986:CDS
TCCTTCCACGTGGCCGCAAC+AGG 0.325588 3.4:+14599929 MS.gene057986:CDS
GTGACCACCGTCGCGGTTCT+CGG 0.360693 3.4:-14598254 None:intergenic
TTACCTCTTCGTAGAAATCT+TGG 0.389702 3.4:-14598382 None:intergenic
CTCTTCTCCTCACCGGCTGC+CGG 0.412074 3.4:+14598228 MS.gene057986:CDS
AATGTGAAGGAATGAGAAAT+TGG 0.412756 3.4:-14598167 None:intergenic
GCACTTCCTCTTTCTCTCCT+CGG 0.413957 3.4:+14598317 MS.gene057986:CDS
GAAGGTGGGGCCCGCAAGGA+AGG 0.432992 3.4:-14598191 None:intergenic
GCCTGTTGCGGCCACGTGGA+AGG 0.472208 3.4:-14599930 None:intergenic
CCGTCGCGGTTCTCGGTCTC+CGG 0.473735 3.4:-14598247 None:intergenic
GGCGAAGAACGAAGCTATCA+TGG 0.476969 3.4:+14599831 MS.gene057986:CDS
TTCACAAGAAGAACAAGAAC+CGG 0.477138 3.4:+14599906 MS.gene057986:CDS
GCGACGGTGGTCACACGTGC+TGG 0.481713 3.4:+14598263 MS.gene057986:CDS
GGTTCTCGGTCTCCGGCAGC+CGG 0.492759 3.4:-14598240 None:intergenic
GGCGGATAGTTGCGTGAAGG+TGG 0.494512 3.4:-14598206 None:intergenic
TTGCGGCCACGTGGAAGGAC+CGG 0.505790 3.4:-14599925 None:intergenic
TGGCCGCAACAGGCCTAAAA+GGG 0.513982 3.4:+14599939 MS.gene057986:CDS
AAGGTGGGGCCCGCAAGGAA+GGG 0.516105 3.4:-14598190 None:intergenic
GTTACACCGAGGAGAGAAAG+AGG 0.516375 3.4:-14598323 None:intergenic
TTTCACTGCTCTTCGAATTG+GGG 0.518302 3.4:+14598349 MS.gene057986:CDS
CGGCAGCCGGTGAGGAGAAG+AGG 0.525624 3.4:-14598227 None:intergenic
CCTTCACATTCCCTTCCTTG+CGG 0.535091 3.4:+14598180 MS.gene057986:CDS
AATTGACGAAGAGGAAGAAG+AGG 0.548103 3.4:+14599855 MS.gene057986:CDS
TCGGTCTCCGGCAGCCGGTG+AGG 0.549955 3.4:-14598235 None:intergenic
GCGTGAAGGTGGGGCCCGCA+AGG 0.554379 3.4:-14598195 None:intergenic
GCGGATAGTTGCGTGAAGGT+GGG 0.555319 3.4:-14598205 None:intergenic
CGGATAGTTGCGTGAAGGTG+GGG 0.580678 3.4:-14598204 None:intergenic
TGAGAAATTGGATTAGAGAG+TGG 0.588473 3.4:-14598155 None:intergenic
TGACCAAGATTTCTACGAAG+AGG 0.594220 3.4:+14598379 MS.gene057986:CDS
TATCATGGAAATTGACGAAG+AGG 0.608853 3.4:+14599846 MS.gene057986:CDS
GAGTGGAAATAACGTCGCCA+TGG 0.612641 3.4:-14598138 None:intergenic
TATCCGCCTCTTCTCCTCAC+CGG 0.636664 3.4:+14598221 MS.gene057986:CDS
TTAGGCCTGTTGCGGCCACG+TGG 0.641712 3.4:-14599934 None:intergenic
GAGACCGAGAACCGCGACGG+TGG 0.655284 3.4:+14598250 MS.gene057986:CDS
CCGGAGACCGAGAACCGCGA+CGG 0.661053 3.4:+14598247 MS.gene057986:CDS
CCGCAAGGAAGGGAATGTGA+AGG 0.673491 3.4:-14598180 None:intergenic
CAGCCGGTGAGGAGAAGAGG+CGG 0.673858 3.4:-14598224 None:intergenic
AGAGGCGGATAGTTGCGTGA+AGG 0.684271 3.4:-14598209 None:intergenic
AGCACGTGTGACCACCGTCG+CGG 0.691929 3.4:-14598261 None:intergenic
CAAGAACCGGTCCTTCCACG+TGG 0.698218 3.4:+14599919 MS.gene057986:CDS
CAGTGAAAACAGTTACACCG+AGG 0.760295 3.4:-14598334 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTATATTAAATATTCATTG+AGG - chr3.4:14599568-14599587 None:intergenic 15.0%
!!! CATCTTTAAAAATAAATCTT+TGG + chr3.4:14599410-14599429 MS.gene057986:intron 15.0%
!! GAAATCTAAATTACAGTAAT+TGG - chr3.4:14599058-14599077 None:intergenic 20.0%
!! TCATGTTAGAAATTTCTTTA+AGG - chr3.4:14599306-14599325 None:intergenic 20.0%
!!! AAAATTGATTTTGAGATGTT+TGG - chr3.4:14598969-14598988 None:intergenic 20.0%
!!! AAATTGATTTTGAGATGTTT+GGG - chr3.4:14598968-14598987 None:intergenic 20.0%
!!! AACTTCAAGTTTATCAATTA+TGG + chr3.4:14598915-14598934 MS.gene057986:intron 20.0%
!!! ATGTTTTTTTTAATACCAGA+TGG + chr3.4:14599810-14599829 MS.gene057986:intron 20.0%
!!! GAAAATTTCTCTTATGTTTT+GGG + chr3.4:14599488-14599507 MS.gene057986:intron 20.0%
!!! TGAAAATTTCTCTTATGTTT+TGG + chr3.4:14599487-14599506 MS.gene057986:intron 20.0%
!!! TGTGTAAAATTGATTGATTT+TGG - chr3.4:14598750-14598769 None:intergenic 20.0%
! AAAAAACATGTCACAAAAAC+AGG - chr3.4:14599799-14599818 None:intergenic 25.0%
! AAAAACATGTCACAAAAACA+GGG - chr3.4:14599798-14599817 None:intergenic 25.0%
! AAACAAAGAATCACATTACT+GGG - chr3.4:14598448-14598467 None:intergenic 25.0%
! AATTAGAAATTTGTGAACAG+AGG - chr3.4:14599711-14599730 None:intergenic 25.0%
! ACAATCTGGAAAATTTCTTA+AGG - chr3.4:14599358-14599377 None:intergenic 25.0%
! AGTAGAATCAAAATCCAAAA+TGG - chr3.4:14598709-14598728 None:intergenic 25.0%
! CAAATATTTCCAAAAAAGTG+TGG - chr3.4:14599757-14599776 None:intergenic 25.0%
! TATTAGTGAGTTAACTGTTT+CGG - chr3.4:14599611-14599630 None:intergenic 25.0%
! TTATAATTACCAGCTCAATA+AGG + chr3.4:14599666-14599685 MS.gene057986:intron 25.0%
! TTTGATGAAATTTCTCAACT+GGG + chr3.4:14598516-14598535 MS.gene057986:intron 25.0%
!! ACACATTTAGAATCACTTAT+GGG + chr3.4:14598765-14598784 MS.gene057986:intron 25.0%
!! TACACATTTAGAATCACTTA+TGG + chr3.4:14598764-14598783 MS.gene057986:intron 25.0%
!!! ACTTACTTTTAGCCTATATT+TGG - chr3.4:14598807-14598826 None:intergenic 25.0%
!!! ATCAATTTTAATTGAGTGCA+AGG - chr3.4:14599261-14599280 None:intergenic 25.0%
!!! CAAAATCGTTTTCTAAAGTT+GGG + chr3.4:14598415-14598434 MS.gene057986:intron 25.0%
AATGTGAAGGAATGAGAAAT+TGG - chr3.4:14598170-14598189 None:intergenic 30.0%
ACAGTTAACTCACTAATATC+TGG + chr3.4:14599613-14599632 MS.gene057986:intron 30.0%
ATAGATATTCCTTATTGAGC+TGG - chr3.4:14599678-14599697 None:intergenic 30.0%
CAAACAAAGAATCACATTAC+TGG - chr3.4:14598449-14598468 None:intergenic 30.0%
GACAAACGATATATCTATCT+AGG + chr3.4:14599156-14599175 MS.gene057986:intron 30.0%
GTTTGATGAAATTTCTCAAC+TGG + chr3.4:14598515-14598534 MS.gene057986:intron 30.0%
TAACAAGTGTTAACAGACAT+AGG - chr3.4:14599132-14599151 None:intergenic 30.0%
TGATGCATTCGTTTATGTAA+AGG + chr3.4:14598850-14598869 MS.gene057986:intron 30.0%
TTTCATCATAACTTGAGAGA+TGG + chr3.4:14599463-14599482 MS.gene057986:intron 30.0%
! CTTCAACTTAGAATCCATTT+TGG + chr3.4:14598692-14598711 MS.gene057986:intron 30.0%
!! CCAACTTTAGAAAACGATTT+TGG - chr3.4:14598417-14598436 None:intergenic 30.0%
!!! CCAAAATCGTTTTCTAAAGT+TGG + chr3.4:14598414-14598433 MS.gene057986:intron 30.0%
!!! GATTCTTAACCACACTTTTT+TGG + chr3.4:14599745-14599764 MS.gene057986:intron 30.0%
!!! TAGAAAACGATTTTGGTTTG+TGG - chr3.4:14598410-14598429 None:intergenic 30.0%
ACATATACGCTCAGCAATAT+AGG + chr3.4:14599220-14599239 MS.gene057986:intron 35.0%
ATACAAGTAGTGTTGTCATC+TGG + chr3.4:14598571-14598590 MS.gene057986:intron 35.0%
CCAAATGTGGAACCAAATAT+AGG + chr3.4:14598792-14598811 MS.gene057986:intron 35.0%
TACAAGTAGTGTTGTCATCT+GGG + chr3.4:14598572-14598591 MS.gene057986:intron 35.0%
TATATCACATCTCGACAATC+TGG - chr3.4:14599372-14599391 None:intergenic 35.0%
TATCATGGAAATTGACGAAG+AGG + chr3.4:14599846-14599865 MS.gene057986:CDS 35.0%
TCACATAGCTAAAATTGCAC+AGG - chr3.4:14599329-14599348 None:intergenic 35.0%
TTACCTCTTCGTAGAAATCT+TGG - chr3.4:14598385-14598404 None:intergenic 35.0%
TTCACAAGAAGAACAAGAAC+CGG + chr3.4:14599906-14599925 MS.gene057986:CDS 35.0%
TTCGTTTATGTAAAGGTGAG+TGG + chr3.4:14598857-14598876 MS.gene057986:intron 35.0%
! ATGGATTCTAAGTTGAAGCT+AGG - chr3.4:14598690-14598709 None:intergenic 35.0%
! CCTATATTTGGTTCCACATT+TGG - chr3.4:14598795-14598814 None:intergenic 35.0%
! GGATAATGATGATGATGATG+TGG + chr3.4:14599876-14599895 MS.gene057986:CDS 35.0%
! TGAGAAATTGGATTAGAGAG+TGG - chr3.4:14598158-14598177 None:intergenic 35.0%
! TTTTCACTGCTCTTCGAATT+GGG + chr3.4:14598348-14598367 MS.gene057986:CDS 35.0%
AATTGACGAAGAGGAAGAAG+AGG + chr3.4:14599855-14599874 MS.gene057986:CDS 40.0%
ACAAGTAGTGTTGTCATCTG+GGG + chr3.4:14598573-14598592 MS.gene057986:intron 40.0%
ACATTCTAAGTCATGCACCA+TGG - chr3.4:14599195-14599214 None:intergenic 40.0%
GGAAGATTAACCAGCTCAAA+AGG - chr3.4:14599097-14599116 None:intergenic 40.0%
TCCACTAAGCAACTCTACAA+CGG - chr3.4:14599025-14599044 None:intergenic 40.0%
TGACCAAGATTTCTACGAAG+AGG + chr3.4:14598379-14598398 MS.gene057986:CDS 40.0%
TTTCACTGCTCTTCGAATTG+GGG + chr3.4:14598349-14598368 MS.gene057986:CDS 40.0%
! GTTTTCACTGCTCTTCGAAT+TGG + chr3.4:14598347-14598366 MS.gene057986:CDS 40.0%
ACTTATGGGTGCTCCAAATG+TGG + chr3.4:14598779-14598798 MS.gene057986:intron 45.0%
AGACATAGGCGCAAGTCAAA+GGG - chr3.4:14599118-14599137 None:intergenic 45.0%
CAAGTAGTGTTGTCATCTGG+GGG + chr3.4:14598574-14598593 MS.gene057986:intron 45.0%
CAGTGAAAACAGTTACACCG+AGG - chr3.4:14598337-14598356 None:intergenic 45.0%
CCAGCTCAAAAGGCTTAGTT+TGG - chr3.4:14599087-14599106 None:intergenic 45.0%
TAACTGATCAGACGTGTGCA+TGG - chr3.4:14599545-14599564 None:intergenic 45.0%
TCCGTTGTAGAGTTGCTTAG+TGG + chr3.4:14599021-14599040 MS.gene057986:intron 45.0%
TGAGATGTTTGGGTGCTCTT+GGG - chr3.4:14598958-14598977 None:intergenic 45.0%
TTGAGATGTTTGGGTGCTCT+TGG - chr3.4:14598959-14598978 None:intergenic 45.0%
! CAGACGTGTGCATGGATTAA+AGG - chr3.4:14599537-14599556 None:intergenic 45.0%
! CCAAACTAAGCCTTTTGAGC+TGG + chr3.4:14599084-14599103 MS.gene057986:intron 45.0%
CAGACATAGGCGCAAGTCAA+AGG - chr3.4:14599119-14599138 None:intergenic 50.0%
CCTTCACATTCCCTTCCTTG+CGG + chr3.4:14598180-14598199 MS.gene057986:CDS 50.0%
CTTCACATTCCCTTCCTTGC+GGG + chr3.4:14598181-14598200 MS.gene057986:CDS 50.0%
GCACTTCCTCTTTCTCTCCT+CGG + chr3.4:14598317-14598336 MS.gene057986:CDS 50.0%
GGCGAAGAACGAAGCTATCA+TGG + chr3.4:14599831-14599850 MS.gene057986:CDS 50.0%
GTTACACCGAGGAGAGAAAG+AGG - chr3.4:14598326-14598345 None:intergenic 50.0%
TAGCTTCGTTCTTCGCCATC+TGG - chr3.4:14599828-14599847 None:intergenic 50.0%
TGTCGCTCACTTGGTTGAGT+AGG + chr3.4:14598602-14598621 MS.gene057986:intron 50.0%
! CTTCCCTTTTAGGCCTGTTG+CGG - chr3.4:14599945-14599964 None:intergenic 50.0%
!! TAGGAGTCTCTGAAGTGCCA+TGG + chr3.4:14599175-14599194 MS.gene057986:intron 50.0%
AGAGGCGGATAGTTGCGTGA+AGG - chr3.4:14598212-14598231 None:intergenic 55.0%
CCGCAAGGAAGGGAATGTGA+AGG - chr3.4:14598183-14598202 None:intergenic 55.0%
CGGATAGTTGCGTGAAGGTG+GGG - chr3.4:14598207-14598226 None:intergenic 55.0%
GCGGATAGTTGCGTGAAGGT+GGG - chr3.4:14598208-14598227 None:intergenic 55.0%
TATCCGCCTCTTCTCCTCAC+CGG + chr3.4:14598221-14598240 MS.gene057986:CDS 55.0%
TGGCCGCAACAGGCCTAAAA+GGG + chr3.4:14599939-14599958 MS.gene057986:CDS 55.0%
CAAGAACCGGTCCTTCCACG+TGG + chr3.4:14599919-14599938 MS.gene057986:CDS 60.0%
GGCGGATAGTTGCGTGAAGG+TGG - chr3.4:14598209-14598228 None:intergenic 60.0%
GTGGCCGCAACAGGCCTAAA+AGG + chr3.4:14599938-14599957 MS.gene057986:CDS 60.0%
! GGGGAGTTGTGTCGCTCACT+TGG + chr3.4:14598593-14598612 MS.gene057986:intron 60.0%
AAGGTGGGGCCCGCAAGGAA+GGG - chr3.4:14598193-14598212 None:intergenic 65.0%
AGCACGTGTGACCACCGTCG+CGG - chr3.4:14598264-14598283 None:intergenic 65.0%
CAGCCGGTGAGGAGAAGAGG+CGG - chr3.4:14598227-14598246 None:intergenic 65.0%
GTGACCACCGTCGCGGTTCT+CGG - chr3.4:14598257-14598276 None:intergenic 65.0%
TCCTTCCACGTGGCCGCAAC+AGG + chr3.4:14599929-14599948 MS.gene057986:CDS 65.0%
TTAGGCCTGTTGCGGCCACG+TGG - chr3.4:14599937-14599956 None:intergenic 65.0%
TTGCGGCCACGTGGAAGGAC+CGG - chr3.4:14599928-14599947 None:intergenic 65.0%
! CTCTTCTCCTCACCGGCTGC+CGG + chr3.4:14598228-14598247 MS.gene057986:CDS 65.0%
CCGGAGACCGAGAACCGCGA+CGG + chr3.4:14598247-14598266 MS.gene057986:CDS 70.0%
CCGTCGCGGTTCTCGGTCTC+CGG - chr3.4:14598250-14598269 None:intergenic 70.0%
CGGCAGCCGGTGAGGAGAAG+AGG - chr3.4:14598230-14598249 None:intergenic 70.0%
GAAGGTGGGGCCCGCAAGGA+AGG - chr3.4:14598194-14598213 None:intergenic 70.0%
GAGACCGAGAACCGCGACGG+TGG + chr3.4:14598250-14598269 MS.gene057986:CDS 70.0%
GCCTGTTGCGGCCACGTGGA+AGG - chr3.4:14599933-14599952 None:intergenic 70.0%
GCGACGGTGGTCACACGTGC+TGG + chr3.4:14598263-14598282 MS.gene057986:CDS 70.0%
GGTTCTCGGTCTCCGGCAGC+CGG - chr3.4:14598243-14598262 None:intergenic 70.0%
GCGTGAAGGTGGGGCCCGCA+AGG - chr3.4:14598198-14598217 None:intergenic 75.0%
TCGGTCTCCGGCAGCCGGTG+AGG - chr3.4:14598238-14598257 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 14598140 14599969 14598140 ID=MS.gene057986
chr3.4 mRNA 14598140 14599969 14598140 ID=MS.gene057986.t1;Parent=MS.gene057986
chr3.4 exon 14598140 14598400 14598140 ID=MS.gene057986.t1.exon1;Parent=MS.gene057986.t1
chr3.4 CDS 14598140 14598400 14598140 ID=cds.MS.gene057986.t1;Parent=MS.gene057986.t1
chr3.4 exon 14599829 14599969 14599829 ID=MS.gene057986.t1.exon2;Parent=MS.gene057986.t1
chr3.4 CDS 14599829 14599969 14599829 ID=cds.MS.gene057986.t1;Parent=MS.gene057986.t1
Gene Sequence

>MS.gene057986

ATGGCGACGTTATTTCCACTCTCTAATCCAATTTCTCATTCCTTCACATTCCCTTCCTTGCGGGCCCCACCTTCACGCAACTATCCGCCTCTTCTCCTCACCGGCTGCCGGAGACCGAGAACCGCGACGGTGGTCACACGTGCTGGCGCAAGTACAAGTAGCTATGCTTTCGCGATTGCACTTCCTCTTTCTCTCCTCGGTGTAACTGTTTTCACTGCTCTTCGAATTGGGGAGAAGCTTGACCAAGATTTCTACGAAGAGGTAATGCCACAAACCAAAATCGTTTTCTAAAGTTGGGCTTTTTTCCCAGTAATGTGATTCTTTGTTTGTGCTGTTAGTTGAAAATTTTGTAGTTTGTTTTTGTGTCTTCTATGTGTTTGATGAAATTTCTCAACTGGGTTTTGTGCAATTGCATACTTGCAATCACTTACATACAAGTAGTGTTGTCATCTGGGGGAGTTGTGTCGCTCACTTGGTTGAGTAGGAATTGTGAGTTGAATAATAAATTTCAGTGTGAATATCACGTTTAGAATCAAACGCTACAAATCCTAGCTTCAACTTAGAATCCATTTTGGATTTTGATTCTACTAGAGAGCAATCAAACATGCCAAAATCAATCAATTTTACACATTTAGAATCACTTATGGGTGCTCCAAATGTGGAACCAAATATAGGCTAAAAGTAAGTTGAAGATAAAGTGATGTATGTTTTGATGCATTCGTTTATGTAAAGGTGAGTGGAACTATAAATTTGAGTGTAAAAAACTCTAATTTCTAACTTCAAGTTTATCAATTATGGAGACAAAATTTATTCTACCCAAGAGCACCCAAACATCTCAAAATCAATTTTATATCTTAGATTTGAAGTTTGCGAATAAGTTCTCCGTTGTAGAGTTGCTTAGTGGATTTTTCTTTTCCAATTACTGTAATTTAGATTTCTATATGCCAAACTAAGCCTTTTGAGCTGGTTAATCTTCCCTTTGACTTGCGCCTATGTCTGTTAACACTTGTTAAACTGACAAACGATATATCTATCTAGGAGTCTCTGAAGTGCCATGGTGCATGACTTAGAATGTGTTTAACATATACGCTCAGCAATATAGGACAAAAACGTGTTTACCTTGCACTCAATTAAAATTGATATGCGCAAAAAATAAAGACGTTCCTTAAAGAAATTTCTAACATGACCTGTGCAATTTTAGCTATGTGATGTCTACCTTAAGAAATTTTCCAGATTGTCGAGATGTGATATATTTAGCATCATATCTTTACATCTTTAAAAATAAATCTTTGGAGTCTAGAGTTAAGAAGATAATTGATAGTTTTTCATCATAACTTGAGAGATGGTTGAAAATTTCTCTTATGTTTTGGGAAACAGAGAATTGTTTGATTGTGCCTTTAATCCATGCACACGTCTGATCAGTTACCTCAATGAATATTTAATATACTTGCATTCACACTCATATGAGCCGAAACAGTTAACTCACTAATATCTGGCATGTTTGTAATATGTGCAAGTGTATGCGATTATAATTACCAGCTCAATAAGGAATATCTATTCTTCACGTGCCTCTGTTCACAAATTTCTAATTTTTAATCTTCATGTGATTCTTAACCACACTTTTTTGGAAATATTTGTCGATTGTTTTTTGTTTTCCCTGTTTTTGTGACATGTTTTTTTTAATACCAGATGGCGAAGAACGAAGCTATCATGGAAATTGACGAAGAGGAAGAAGAGGATAATGATGATGATGATGTGGAAACTTATTCACAAGAAGAACAAGAACCGGTCCTTCCACGTGGCCGCAACAGGCCTAAAAGGGAAGCTTAA

Protein sequence

>MS.gene057986.t1

MATLFPLSNPISHSFTFPSLRAPPSRNYPPLLLTGCRRPRTATVVTRAGASTSSYAFAIALPLSLLGVTVFTALRIGEKLDQDFYEEMAKNEAIMEIDEEEEEDNDDDDVETYSQEEQEPVLPRGRNRPKREA