AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07059


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07059.t1 MTR_7g109550 97.581 124 3 0 1 124 197 320 1.65e-86 260
MS.gene07059.t1 MTR_7g094750 57.377 122 50 1 1 120 151 272 4.68e-41 139
MS.gene07059.t1 MTR_2g022580 50.400 125 60 1 1 123 2 126 2.08e-40 131
MS.gene07059.t1 MTR_7g094750 57.377 122 50 1 1 120 151 272 3.13e-40 139
MS.gene07059.t1 MTR_5g033800 50.407 123 61 0 1 123 205 327 2.95e-39 135
MS.gene07059.t1 MTR_7g093450 52.800 125 56 2 1 123 308 431 4.54e-39 138
MS.gene07059.t1 MTR_8g069790 50.407 123 61 0 1 123 238 360 6.29e-39 137
MS.gene07059.t1 MTR_2g022560 51.639 122 57 1 1 120 157 278 1.93e-38 135
MS.gene07059.t1 MTR_5g033800 50.407 123 61 0 1 123 205 327 2.86e-38 135
MS.gene07059.t1 MTR_0136s0010 51.613 124 59 1 1 123 230 353 3.53e-38 135
MS.gene07059.t1 MTR_5g059400 47.200 125 63 2 1 123 235 358 7.01e-38 134
MS.gene07059.t1 MTR_1g116130 47.200 125 63 2 1 123 235 358 7.01e-38 134
MS.gene07059.t1 MTR_4g126950 50.400 125 61 1 1 124 169 293 2.14e-36 129
MS.gene07059.t1 MTR_4g121560 48.000 125 63 1 1 123 175 299 3.87e-36 129
MS.gene07059.t1 MTR_3g090120 46.281 121 63 2 1 119 193 313 1.26e-30 114
MS.gene07059.t1 MTR_8g093530 45.455 121 64 1 1 119 150 270 1.09e-29 111
MS.gene07059.t1 MTR_1g058380 41.732 127 71 2 1 124 117 243 1.93e-28 107
MS.gene07059.t1 MTR_3g464570 36.719 128 70 4 5 132 3 119 2.76e-23 91.7
MS.gene07059.t1 MTR_3g464570 38.655 119 62 4 5 123 107 214 1.19e-22 91.7
MS.gene07059.t1 MTR_5g071350 36.975 119 64 4 5 123 122 229 6.61e-21 87.0
MS.gene07059.t1 MTR_3g070900 41.935 124 59 4 1 120 167 281 8.00e-21 87.0
MS.gene07059.t1 MTR_1g112210 34.513 113 64 2 5 117 122 224 6.05e-20 84.3
MS.gene07059.t1 MTR_4g016940 35.345 116 66 2 2 115 148 256 1.08e-19 83.6
MS.gene07059.t1 MTR_2g015720 35.938 128 70 3 6 133 106 221 5.42e-19 81.3
MS.gene07059.t1 MTR_4g014440 37.288 118 65 3 1 115 123 234 2.16e-18 80.1
MS.gene07059.t1 MTR_1g050448 35.088 114 62 3 5 117 116 218 2.44e-18 79.7
MS.gene07059.t1 MTR_2g073510 40.000 115 55 5 2 115 95 196 1.63e-17 77.4
MS.gene07059.t1 MTR_5g082880 41.803 122 55 5 1 115 226 338 1.83e-17 77.8
MS.gene07059.t1 MTR_7g070060 38.462 117 65 3 1 115 125 236 2.37e-17 76.6
MS.gene07059.t1 MTR_5g034810 34.711 121 67 3 1 121 93 201 3.26e-17 76.6
MS.gene07059.t1 MTR_7g070060 38.462 117 65 3 1 115 23 134 3.32e-17 75.9
MS.gene07059.t1 MTR_2g073500 33.333 120 62 4 2 117 96 201 3.71e-17 76.3
MS.gene07059.t1 MTR_3g021060 35.338 133 72 6 2 133 107 226 4.00e-17 76.3
MS.gene07059.t1 MTR_7g070060 38.462 117 65 3 1 115 125 236 4.87e-17 76.3
MS.gene07059.t1 MTR_2g091175 37.815 119 59 5 2 117 108 214 6.16e-17 75.9
MS.gene07059.t1 MTR_1g112210 34.513 113 51 3 5 117 122 211 6.57e-17 75.9
MS.gene07059.t1 MTR_4g014430 37.398 123 68 2 1 121 169 284 8.04e-17 75.9
MS.gene07059.t1 MTR_7g068920 33.051 118 66 3 2 115 142 250 2.97e-16 73.9
MS.gene07059.t1 MTR_3g071650 38.136 118 62 3 1 115 150 259 3.00e-16 73.9
MS.gene07059.t1 MTR_4g079700 34.783 115 57 4 6 117 112 211 1.46e-15 72.0
MS.gene07059.t1 MTR_5g083260 35.043 117 67 2 1 115 144 253 1.78e-15 72.0
MS.gene07059.t1 MTR_8g463790 32.520 123 66 4 1 117 132 243 2.34e-15 71.6
MS.gene07059.t1 MTR_4g087730 31.356 118 69 3 6 123 110 215 2.43e-15 71.2
MS.gene07059.t1 MTR_1g111010 36.441 118 62 6 1 117 94 199 5.79e-15 70.1
MS.gene07059.t1 MTR_3g021090 33.333 114 64 3 2 115 92 193 1.25e-14 69.3
MS.gene07059.t1 MTR_5g069320 32.500 120 53 4 5 123 165 257 3.42e-14 68.2
MS.gene07059.t1 MTR_3g035830 32.727 110 62 3 6 115 96 193 5.06e-14 67.4
MS.gene07059.t1 MTR_3g049330 34.959 123 66 5 6 127 96 205 1.85e-13 65.9
MS.gene07059.t1 MTR_4g088030 34.821 112 61 5 6 117 98 197 1.35e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07059.t1 AT3G62390 78.226 124 27 0 1 124 198 321 6.93e-71 218
MS.gene07059.t1 AT3G62390 78.226 124 27 0 1 124 198 321 7.22e-70 217
MS.gene07059.t1 AT5G06700 53.600 125 55 2 1 123 315 438 3.19e-42 147
MS.gene07059.t1 AT5G19160 55.200 125 54 1 1 123 159 283 3.43e-42 142
MS.gene07059.t1 AT3G06080 55.738 122 52 1 1 120 163 284 1.22e-41 141
MS.gene07059.t1 AT5G19160 55.200 125 54 1 1 123 159 283 3.00e-41 142
MS.gene07059.t1 AT1G60790 55.645 124 54 1 1 123 252 375 6.24e-41 142
MS.gene07059.t1 AT3G06080 55.738 122 52 1 1 120 163 284 6.44e-41 141
MS.gene07059.t1 AT3G12060 53.600 125 55 2 1 123 256 379 2.85e-40 141
MS.gene07059.t1 AT5G20590 55.000 120 53 1 5 123 206 325 1.74e-39 136
MS.gene07059.t1 AT5G20590 55.000 120 53 1 5 123 206 325 8.45e-39 136
MS.gene07059.t1 AT5G49340 47.619 126 63 3 1 123 160 285 1.42e-33 122
MS.gene07059.t1 AT5G06230 45.902 122 64 1 1 120 128 249 2.46e-31 113
MS.gene07059.t1 AT5G06230 45.902 122 64 1 1 120 87 208 5.36e-31 114
MS.gene07059.t1 AT5G06230 45.902 122 64 1 1 120 128 249 1.06e-30 113
MS.gene07059.t1 AT3G11570 45.902 122 64 1 1 120 142 263 6.51e-30 111
MS.gene07059.t1 AT1G48880 43.802 121 66 1 1 119 175 295 4.57e-29 109
MS.gene07059.t1 AT5G58600 37.398 123 66 4 5 127 131 242 1.12e-23 93.2
MS.gene07059.t1 AT5G58600 37.398 123 66 4 5 127 131 242 3.34e-23 93.2
MS.gene07059.t1 AT3G54260 37.391 115 62 2 5 119 114 218 8.43e-22 89.4
MS.gene07059.t1 AT2G40160 38.400 125 63 4 1 120 138 253 1.97e-21 87.4
MS.gene07059.t1 AT3G55990 43.697 119 54 4 1 115 201 310 2.22e-21 88.6
MS.gene07059.t1 AT2G38320 36.029 136 75 4 1 133 37 163 2.88e-21 87.4
MS.gene07059.t1 AT2G40160 38.400 125 63 4 1 120 138 253 5.74e-21 87.4
MS.gene07059.t1 AT2G40150 42.017 119 56 4 1 115 132 241 6.32e-21 87.4
MS.gene07059.t1 AT2G38320 37.288 118 65 3 1 115 120 231 6.78e-21 87.0
MS.gene07059.t1 AT2G30010 37.903 124 65 3 1 123 118 230 1.08e-20 86.3
MS.gene07059.t1 AT2G30010 39.316 117 59 3 2 117 162 267 1.28e-20 86.7
MS.gene07059.t1 AT2G40160 38.400 125 63 4 1 120 138 253 2.24e-20 85.9
MS.gene07059.t1 AT1G29050 37.288 118 63 2 6 123 126 232 4.02e-20 84.7
MS.gene07059.t1 AT2G34070 37.607 117 62 2 6 122 130 235 1.20e-19 83.6
MS.gene07059.t1 AT2G42570 35.398 113 61 3 5 117 111 211 1.05e-18 80.9
MS.gene07059.t1 AT5G01360 35.088 114 63 3 5 115 101 206 2.28e-18 79.7
MS.gene07059.t1 AT5G01360 35.088 114 63 3 5 115 157 262 2.51e-18 80.1
MS.gene07059.t1 AT5G01620 39.516 124 64 3 1 121 172 287 4.91e-18 79.3
MS.gene07059.t1 AT5G01620 39.516 124 64 3 1 121 172 287 4.91e-18 79.3
MS.gene07059.t1 AT5G01620 39.516 124 64 3 1 121 180 295 5.61e-18 79.0
MS.gene07059.t1 AT3G11030 35.897 117 66 2 1 115 167 276 2.12e-17 77.4
MS.gene07059.t1 AT2G34070 35.714 112 61 2 11 122 1 101 2.29e-17 75.9
MS.gene07059.t1 AT2G30900 36.441 118 64 4 4 121 107 213 2.69e-17 75.9
MS.gene07059.t1 AT1G78710 34.483 116 65 3 2 117 91 195 4.98e-17 75.5
MS.gene07059.t1 AT2G30900 36.441 118 64 4 4 121 107 213 8.79e-17 75.5
MS.gene07059.t1 AT1G78710 34.188 117 66 3 1 117 97 202 1.07e-16 75.1
MS.gene07059.t1 AT1G73140 33.065 124 66 4 1 118 128 240 1.12e-16 75.1
MS.gene07059.t1 AT2G31110 33.628 113 63 4 5 117 109 209 1.82e-15 70.9
MS.gene07059.t1 AT2G31110 33.628 113 63 4 5 117 109 209 3.94e-15 70.9
MS.gene07059.t1 AT2G40320 32.479 117 70 2 1 115 142 251 5.12e-15 70.5
MS.gene07059.t1 AT3G14850 31.111 135 78 5 2 133 92 214 9.41e-15 69.7
MS.gene07059.t1 AT3G14850 30.894 123 73 4 11 133 1 111 1.20e-12 63.2
MS.gene07059.t1 AT1G70230 38.333 120 60 6 2 117 140 249 6.13e-11 58.9

Find 26 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACTATAGTTTCAGGTTTC+TGG 0.139405 7.2:+5749462 MS.gene07059:CDS
TTCCCATTGGTTCCTATTAA+TGG 0.150183 7.2:-5749353 None:intergenic
ATGGAGAGGAGCTGATATTT+TGG 0.261754 7.2:+5749701 MS.gene07059:CDS
AGAGAGGGAACTATAGTTTC+AGG 0.285584 7.2:+5749455 MS.gene07059:CDS
TTGTGGGTGATTCCATTAAT+AGG 0.313259 7.2:+5749341 MS.gene07059:CDS
TGGAGTTTATAAGAGGAAAA+AGG 0.315433 7.2:+5749311 MS.gene07059:CDS
ACTATAGTTCCCTCTCTCTT+TGG 0.323759 7.2:-5749449 None:intergenic
CGTGCCTCATACACTCTCTT+TGG 0.387282 7.2:-5749412 None:intergenic
CGAATTGATGCGATTGATCA+TGG 0.420809 7.2:+5749669 MS.gene07059:CDS
GAGAAGTGCTAACCCAGCTT+TGG 0.426335 7.2:-5749760 None:intergenic
AAAATGTTGGAGTTTATAAG+AGG 0.432567 7.2:+5749304 None:intergenic
ATCGCATCAATTCGCAAAGT+TGG 0.456158 7.2:-5749660 None:intergenic
AGGAAAATTACCAAAGAGAG+AGG 0.476594 7.2:+5749439 MS.gene07059:CDS
TCACATCACAAAACCAAAGC+TGG 0.490317 7.2:+5749747 MS.gene07059:CDS
TTGATCATGGTTCGTCAAGA+TGG 0.494455 7.2:+5749682 MS.gene07059:CDS
ATTCCATTAATAGGAACCAA+TGG 0.514445 7.2:+5749350 MS.gene07059:CDS
TGCACAACATTGATTCCCAT+TGG 0.516029 7.2:-5749366 None:intergenic
CATGAAAGCAAAGCAAGAGT+AGG 0.524542 7.2:+5749624 MS.gene07059:CDS
AGATCCAAAGAGAGTGTATG+AGG 0.528363 7.2:+5749408 MS.gene07059:CDS
TTCCATTAATAGGAACCAAT+GGG 0.535430 7.2:+5749351 MS.gene07059:CDS
ATGAAAGCAAAGCAAGAGTA+GGG 0.551438 7.2:+5749625 MS.gene07059:CDS
CACATCACAAAACCAAAGCT+GGG 0.583781 7.2:+5749748 MS.gene07059:CDS
GAGTGTATGAGGCACGTGGA+AGG 0.601700 7.2:+5749419 MS.gene07059:CDS
CATGGTTCGTCAAGATGGAG+AGG 0.632218 7.2:+5749687 MS.gene07059:CDS
GGAAAATTACCAAAGAGAGA+GGG 0.638676 7.2:+5749440 MS.gene07059:CDS
AAGAGAGTGTATGAGGCACG+TGG 0.685399 7.2:+5749415 MS.gene07059:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGAACAAATATCAAATATTT+TGG - chr7.2:5749543-5749562 None:intergenic 15.0%
!!! GAACAAATATCAAATATTTT+GGG - chr7.2:5749542-5749561 None:intergenic 15.0%
!! AATGTATAATTGTATATGAC+TGG + chr7.2:5749515-5749534 MS.gene07059:intron 20.0%
!! TAATTATTCAGAACTTTACT+TGG + chr7.2:5749485-5749504 MS.gene07059:intron 20.0%
ATTCCATTAATAGGAACCAA+TGG + chr7.2:5749350-5749369 MS.gene07059:CDS 30.0%
TGGAGTTTATAAGAGGAAAA+AGG + chr7.2:5749311-5749330 MS.gene07059:CDS 30.0%
TTCCATTAATAGGAACCAAT+GGG + chr7.2:5749351-5749370 MS.gene07059:CDS 30.0%
AGGAAAATTACCAAAGAGAG+AGG + chr7.2:5749439-5749458 MS.gene07059:CDS 35.0%
ATGAAAGCAAAGCAAGAGTA+GGG + chr7.2:5749625-5749644 MS.gene07059:CDS 35.0%
GGAAAATTACCAAAGAGAGA+GGG + chr7.2:5749440-5749459 MS.gene07059:CDS 35.0%
TGTTCTTTCTGCAACTTTGT+AGG + chr7.2:5749557-5749576 MS.gene07059:intron 35.0%
TTGTGGGTGATTCCATTAAT+AGG + chr7.2:5749341-5749360 MS.gene07059:CDS 35.0%
!! GAACTATAGTTTCAGGTTTC+TGG + chr7.2:5749462-5749481 MS.gene07059:CDS 35.0%
!! TTCCCATTGGTTCCTATTAA+TGG - chr7.2:5749356-5749375 None:intergenic 35.0%
!!! AGGAAAAAGGCTAGTTTTTG+TGG + chr7.2:5749324-5749343 MS.gene07059:CDS 35.0%
!!! GGAAAAAGGCTAGTTTTTGT+GGG + chr7.2:5749325-5749344 MS.gene07059:CDS 35.0%
ACTATAGTTCCCTCTCTCTT+TGG - chr7.2:5749452-5749471 None:intergenic 40.0%
AGAGAGGGAACTATAGTTTC+AGG + chr7.2:5749455-5749474 MS.gene07059:CDS 40.0%
ATCGCATCAATTCGCAAAGT+TGG - chr7.2:5749663-5749682 None:intergenic 40.0%
CACATCACAAAACCAAAGCT+GGG + chr7.2:5749748-5749767 MS.gene07059:CDS 40.0%
CATGAAAGCAAAGCAAGAGT+AGG + chr7.2:5749624-5749643 MS.gene07059:CDS 40.0%
CGAATTGATGCGATTGATCA+TGG + chr7.2:5749669-5749688 MS.gene07059:CDS 40.0%
TCACATCACAAAACCAAAGC+TGG + chr7.2:5749747-5749766 MS.gene07059:CDS 40.0%
TTGATCATGGTTCGTCAAGA+TGG + chr7.2:5749682-5749701 MS.gene07059:CDS 40.0%
! AGATCCAAAGAGAGTGTATG+AGG + chr7.2:5749408-5749427 MS.gene07059:CDS 40.0%
! ATGGAGAGGAGCTGATATTT+TGG + chr7.2:5749701-5749720 MS.gene07059:CDS 40.0%
! TGGTTTTCAACACAGCACAT+TGG + chr7.2:5749721-5749740 MS.gene07059:CDS 40.0%
! TTTTCAACACAGCACATTGG+TGG + chr7.2:5749724-5749743 MS.gene07059:CDS 40.0%
!! TGCACAACATTGATTCCCAT+TGG - chr7.2:5749369-5749388 None:intergenic 40.0%
CATGGTTCGTCAAGATGGAG+AGG + chr7.2:5749687-5749706 MS.gene07059:CDS 50.0%
CGTGCCTCATACACTCTCTT+TGG - chr7.2:5749415-5749434 None:intergenic 50.0%
! AAGAGAGTGTATGAGGCACG+TGG + chr7.2:5749415-5749434 MS.gene07059:CDS 50.0%
!! GAGAAGTGCTAACCCAGCTT+TGG - chr7.2:5749763-5749782 None:intergenic 50.0%
GAGTGTATGAGGCACGTGGA+AGG + chr7.2:5749419-5749438 MS.gene07059:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 5749307 5749803 5749307 ID=MS.gene07059
chr7.2 mRNA 5749307 5749803 5749307 ID=MS.gene07059.t1;Parent=MS.gene07059
chr7.2 exon 5749307 5749483 5749307 ID=MS.gene07059.t1.exon1;Parent=MS.gene07059.t1
chr7.2 CDS 5749307 5749483 5749307 ID=cds.MS.gene07059.t1;Parent=MS.gene07059.t1
chr7.2 exon 5749579 5749803 5749579 ID=MS.gene07059.t1.exon2;Parent=MS.gene07059.t1
chr7.2 CDS 5749579 5749803 5749579 ID=cds.MS.gene07059.t1;Parent=MS.gene07059.t1
Gene Sequence

>MS.gene07059

ATGTTGGAGTTTATAAGAGGAAAAAGGCTAGTTTTTGTGGGTGATTCCATTAATAGGAACCAATGGGAATCAATGTTGTGCATGTTATTGAGTGCTGTTAAAGATCCAAAGAGAGTGTATGAGGCACGTGGAAGGAAAATTACCAAAGAGAGAGGGAACTATAGTTTCAGGTTTCTGGTAATTATTCAGAACTTTACTTGGTTTGTGTAATGTATAATTGTATATGACTGGTCCCAAAATATTTGATATTTGTTCTTTCTGCAACTTTGTAGGACTATCAATGTACAGTTGAATACTATGTGAGTCATTTCTTAGTTCATGAAAGCAAAGCAAGAGTAGGGCAGAAACGAAGACCAACTTTGCGAATTGATGCGATTGATCATGGTTCGTCAAGATGGAGAGGAGCTGATATTTTGGTTTTCAACACAGCACATTGGTGGTCACATCACAAAACCAAAGCTGGGTTAGCACTTCTCTCTATCTTTTCGAAATTGTAG

Protein sequence

>MS.gene07059.t1

MLEFIRGKRLVFVGDSINRNQWESMLCMLLSAVKDPKRVYEARGRKITKERGNYSFRFLDYQCTVEYYVSHFLVHESKARVGQKRRPTLRIDAIDHGSSRWRGADILVFNTAHWWSHHKTKAGLALLSIFSKL