Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070819.t1 | XP_013444865.1 | 94.4 | 72 | 4 | 0 | 1 | 72 | 1 | 72 | 4.50E-31 | 143.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070819.t1 | G7KQ30 | 94.4 | 72 | 4 | 0 | 1 | 72 | 1 | 72 | 3.3e-31 | 143.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050807 | MS.gene070819 | 0.802917 | 4.47E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene070819 | 0.803621 | 3.19E-49 | -1.69E-46 |
| MS.gene052302 | MS.gene070819 | 0.800668 | 1.29E-48 | -1.69E-46 |
| MS.gene052856 | MS.gene070819 | 0.801679 | 8.03E-49 | -1.69E-46 |
| MS.gene052858 | MS.gene070819 | 0.805521 | 1.28E-49 | -1.69E-46 |
| MS.gene052860 | MS.gene070819 | 0.812288 | 4.57E-51 | -1.69E-46 |
| MS.gene058399 | MS.gene070819 | 0.808028 | 3.79E-50 | -1.69E-46 |
| MS.gene060480 | MS.gene070819 | 0.822907 | 1.84E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070819.t1 | MTR_8g031320 | 94.444 | 72 | 4 | 0 | 1 | 72 | 1 | 72 | 3.87e-48 | 147 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070819.t1 | AT5G44710 | 56.944 | 72 | 31 | 0 | 1 | 72 | 1 | 72 | 4.33e-30 | 102 |
Find 15 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTCATCAATTGAATCCTTC+AGG | 0.310963 | 8.1:-49048296 | MS.gene070819:CDS |
| ATTCTCCGTATGAAAATGTT+TGG | 0.383146 | 8.1:-49048254 | MS.gene070819:CDS |
| TCATCACGAGCAACGATATT+CGG | 0.393637 | 8.1:-49048317 | MS.gene070819:CDS |
| TAAAGATGATCCCAATGTTA+TGG | 0.410197 | 8.1:-49048195 | MS.gene070819:CDS |
| TGTTGACGAGCCATAACATT+GGG | 0.470795 | 8.1:+49048185 | None:intergenic |
| GTCATCAATTGAATCCTTCA+GGG | 0.500225 | 8.1:-49048295 | MS.gene070819:CDS |
| TCATCTTTATTGATATCATG+AGG | 0.545670 | 8.1:+49048209 | None:intergenic |
| TGAGCAGATTTCTTCCCTGA+AGG | 0.553711 | 8.1:+49048281 | None:intergenic |
| ATGTTAACCTCTGCAATCAA+CGG | 0.613390 | 8.1:-49048350 | MS.gene070819:CDS |
| CATCTTTATTGATATCATGA+GGG | 0.630102 | 8.1:+49048210 | None:intergenic |
| TTGTTGACGAGCCATAACAT+TGG | 0.647703 | 8.1:+49048184 | None:intergenic |
| AATACCGCCGTTGATTGCAG+AGG | 0.659011 | 8.1:+49048343 | None:intergenic |
| AGAACGAAGAAGATGGCTAG+CGG | 0.719343 | 8.1:-49048386 | None:intergenic |
| ACGAAGAAGATGGCTAGCGG+CGG | 0.720581 | 8.1:-49048383 | None:intergenic |
| TTAACCTCTGCAATCAACGG+CGG | 0.723215 | 8.1:-49048347 | MS.gene070819:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CATCTTTATTGATATCATGA+GGG | + | chr8.1:49048342-49048361 | None:intergenic | 25.0% |
| ! | TCATCTTTATTGATATCATG+AGG | + | chr8.1:49048343-49048362 | None:intergenic | 25.0% |
| !! | TTTCACCAAACATTTTCATA+CGG | + | chr8.1:49048303-49048322 | None:intergenic | 25.0% |
| ATTCTCCGTATGAAAATGTT+TGG | - | chr8.1:49048295-49048314 | MS.gene070819:CDS | 30.0% | |
| TAAAGATGATCCCAATGTTA+TGG | - | chr8.1:49048354-49048373 | MS.gene070819:CDS | 30.0% | |
| TTGGTGAAAAAGTTACTCAA+TGG | - | chr8.1:49048314-49048333 | MS.gene070819:CDS | 30.0% | |
| ATGTTAACCTCTGCAATCAA+CGG | - | chr8.1:49048199-49048218 | MS.gene070819:CDS | 35.0% | |
| GTCATCAATTGAATCCTTCA+GGG | - | chr8.1:49048254-49048273 | MS.gene070819:CDS | 35.0% | |
| GGTCATCAATTGAATCCTTC+AGG | - | chr8.1:49048253-49048272 | MS.gene070819:CDS | 40.0% | |
| TCATCACGAGCAACGATATT+CGG | - | chr8.1:49048232-49048251 | MS.gene070819:CDS | 40.0% | |
| TGTTGACGAGCCATAACATT+GGG | + | chr8.1:49048367-49048386 | None:intergenic | 40.0% | |
| TTGTTGACGAGCCATAACAT+TGG | + | chr8.1:49048368-49048387 | None:intergenic | 40.0% | |
| TGAGCAGATTTCTTCCCTGA+AGG | + | chr8.1:49048271-49048290 | None:intergenic | 45.0% | |
| TTAACCTCTGCAATCAACGG+CGG | - | chr8.1:49048202-49048221 | MS.gene070819:CDS | 45.0% | |
| ! | AATACCGCCGTTGATTGCAG+AGG | + | chr8.1:49048209-49048228 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 49048175 | 49048396 | 49048175 | ID=MS.gene070819 |
| chr8.1 | mRNA | 49048175 | 49048396 | 49048175 | ID=MS.gene070819.t1;Parent=MS.gene070819 |
| chr8.1 | exon | 49048175 | 49048396 | 49048175 | ID=MS.gene070819.t1.exon1;Parent=MS.gene070819.t1 |
| chr8.1 | CDS | 49048175 | 49048396 | 49048175 | ID=cds.MS.gene070819.t1;Parent=MS.gene070819.t1 |
| Gene Sequence |
| Protein sequence |
