Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071697.t1 | PNY04801.1 | 88.8 | 80 | 9 | 0 | 8 | 87 | 407 | 486 | 6.30E-33 | 149.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071697.t1 | Q8L5Y9 | 86.2 | 58 | 8 | 0 | 8 | 65 | 408 | 465 | 1.3e-23 | 110.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071697.t1 | A0A2K3NP46 | 88.8 | 80 | 9 | 0 | 8 | 87 | 407 | 486 | 4.5e-33 | 149.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053621 | MS.gene071697 | 0.802801 | 4.72E-49 | -1.69E-46 |
| MS.gene05417 | MS.gene071697 | 0.824544 | 7.62E-54 | -1.69E-46 |
| MS.gene054236 | MS.gene071697 | 0.806007 | 1.01E-49 | -1.69E-46 |
| MS.gene054467 | MS.gene071697 | 0.809226 | 2.10E-50 | -1.69E-46 |
| MS.gene057869 | MS.gene071697 | 0.804758 | 1.85E-49 | -1.69E-46 |
| MS.gene058010 | MS.gene071697 | 0.804289 | 2.32E-49 | -1.69E-46 |
| MS.gene058515 | MS.gene071697 | 0.802708 | 4.94E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene071697 | 0.80862 | 2.83E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071697.t1 | MTR_3g077740 | 91.379 | 58 | 5 | 0 | 8 | 65 | 401 | 458 | 3.35e-31 | 115 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071697.t1 | AT4G32180 | 86.207 | 58 | 8 | 0 | 8 | 65 | 408 | 465 | 1.81e-30 | 113 |
| MS.gene071697.t1 | AT4G32180 | 86.207 | 58 | 8 | 0 | 8 | 65 | 408 | 465 | 1.81e-30 | 113 |
| MS.gene071697.t1 | AT4G32180 | 86.207 | 58 | 8 | 0 | 8 | 65 | 408 | 465 | 5.76e-29 | 108 |
Find 0 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATTATCTTGAGCAGGTAAT+CGG | - | 21148:7572-7591 | MS.gene071697:intergenic | 30.0% | |
| TTTATGAGTTACGAAAAGCA+TGG | + | 21148:7406-7425 | MS.gene071697:CDS | 30.0% | |
| AACATCCAATTATCTTGAGC+AGG | - | 21148:7579-7598 | MS.gene071696:intergenic | 35.0% | |
| GATTACCTGCTCAAGATAAT+TGG | + | 21148:7571-7590 | MS.gene071697:CDS | 35.0% | |
| GGATGTTCTTGTTCTTTAGT+TGG | + | 21148:7592-7611 | MS.gene071697:CDS | 35.0% | |
| TTAGTTGAAAGGTTTCCGAT+GGG | + | 21148:7454-7473 | MS.gene071697:CDS | 35.0% | |
| ACTTGGCGATTTGAATGAGA+AGG | + | 21148:7513-7532 | MS.gene071697:CDS | 40.0% | |
| GTTAGTTGAAAGGTTTCCGA+TGG | + | 21148:7453-7472 | MS.gene071697:CDS | 40.0% | |
| TATACTGGTGGCAAGATACA+TGG | + | 21148:7484-7503 | MS.gene071697:CDS | 40.0% | |
| TGGTGCATCAGTTAGTTGAA+AGG | + | 21148:7443-7462 | MS.gene071697:CDS | 40.0% | |
| TGTATCTTGCCACCAGTATA+TGG | - | 21148:7484-7503 | MS.gene071697:intergenic | 40.0% | |
| TTCTCATTCAAATCGCCAAG+TGG | - | 21148:7514-7533 | MS.gene071697:intergenic | 40.0% | |
| !! | CGATGTTTTTGCGACATGAA+GGG | + | 21148:7361-7380 | MS.gene071697:CDS | 40.0% |
| ATGTCGCAAAAACATCGCCT+GGG | - | 21148:7358-7377 | MS.gene071697:intergenic | 45.0% | |
| CAACTAACTGATGCACCATG+AGG | - | 21148:7441-7460 | MS.gene071697:intergenic | 45.0% | |
| CATTCAAATCGCCAAGTGGT+GGG | - | 21148:7510-7529 | MS.gene071697:intergenic | 45.0% | |
| GCATGGTTTAGATGACCTCA+TGG | + | 21148:7423-7442 | MS.gene071697:CDS | 45.0% | |
| TCATTCAAATCGCCAAGTGG+TGG | - | 21148:7511-7530 | MS.gene071697:intergenic | 45.0% | |
| TGCGACATGAAGGGATTTCT+GGG | + | 21148:7370-7389 | MS.gene071697:CDS | 45.0% | |
| TTGCGACATGAAGGGATTTC+TGG | + | 21148:7369-7388 | MS.gene071697:CDS | 45.0% | |
| ! | AAGGGATTTCTGGGAGCTTT+AGG | + | 21148:7379-7398 | MS.gene071697:CDS | 45.0% |
| !! | GCGATGTTTTTGCGACATGA+AGG | + | 21148:7360-7379 | MS.gene071697:CDS | 45.0% |
| AAGATACATGGCCCACCACT+TGG | + | 21148:7496-7515 | MS.gene071697:CDS | 50.0% | |
| ATGGGTGCTCCATATACTGG+TGG | + | 21148:7472-7491 | MS.gene071697:CDS | 50.0% | |
| CATGTCGCAAAAACATCGCC+TGG | - | 21148:7359-7378 | MS.gene071697:intergenic | 50.0% | |
| ! | CCAGTATATGGAGCACCCAT+CGG | - | 21148:7472-7491 | MS.gene071697:intergenic | 50.0% |
| CCGATGGGTGCTCCATATAC+TGG | + | 21148:7469-7488 | MS.gene071697:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 21148 | gene | 7346 | 7633 | 7346 | ID=MS.gene071697 |
| 21148 | mRNA | 7346 | 7633 | 7346 | ID=MS.gene071697.t1;Parent=MS.gene071697 |
| 21148 | exon | 7346 | 7633 | 7346 | ID=MS.gene071697.t1.exon1;Parent=MS.gene071697.t1 |
| 21148 | CDS | 7346 | 7633 | 7346 | ID=cds.MS.gene071697.t1;Parent=MS.gene071697.t1 |
| Gene Sequence |
| Protein sequence |
