AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071737


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071737.t1 MTR_8g076780 99.206 126 1 0 1 126 70 195 3.56e-81 236
MS.gene071737.t1 MTR_2g031960 53.043 115 52 1 14 126 41 155 1.41e-30 107
MS.gene071737.t1 MTR_2g031760 61.386 101 34 1 26 126 95 190 1.63e-29 105
MS.gene071737.t1 MTR_2g064300 51.304 115 47 2 20 126 103 216 7.90e-27 99.4
MS.gene071737.t1 MTR_7g053520 55.046 109 40 2 26 126 110 217 5.40e-26 97.4
MS.gene071737.t1 MTR_6g006060 45.714 70 38 0 56 125 111 180 7.83e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071737.t1 AT4G36420 61.111 126 42 3 1 126 61 179 9.15e-39 129
MS.gene071737.t1 AT3G06040 63.441 93 30 1 34 126 98 186 6.98e-30 106
MS.gene071737.t1 AT3G06040 63.441 93 30 1 34 126 98 186 6.98e-30 106
MS.gene071737.t1 AT3G06040 63.441 93 30 1 34 126 98 186 6.98e-30 106
MS.gene071737.t1 AT1G70190 51.639 122 53 3 6 126 92 208 1.33e-24 93.6
MS.gene071737.t1 AT1G70190 51.639 122 53 3 6 126 92 208 1.33e-24 93.6
MS.gene071737.t1 AT4G37660 69.118 68 21 0 59 126 100 167 2.56e-24 91.7
MS.gene071737.t1 AT2G03130 48.315 89 42 1 38 126 7 91 2.52e-15 67.0
MS.gene071737.t1 AT3G27830 52.055 73 35 0 53 125 118 190 3.27e-15 68.6
MS.gene071737.t1 AT3G27830 52.055 73 35 0 53 125 118 190 3.27e-15 68.6
MS.gene071737.t1 AT3G27850 52.055 73 35 0 53 125 114 186 3.53e-15 68.6
MS.gene071737.t1 AT3G27840 43.210 81 46 0 45 125 112 192 1.64e-13 64.3

Find 36 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGTAATGCGAGAGAAAA+AGG 0.238846 8.1:-31167399 MS.gene071737:CDS
GGGGATTATTGCTAAGTTAA+AGG 0.252904 8.1:-31167081 MS.gene071737:CDS
TGGTTCCTGGAATGGGAATA+AGG 0.262636 8.1:-31167346 MS.gene071737:CDS
AGGTGTAGGTGGAGGGAAAG+GGG 0.337630 8.1:-31167312 MS.gene071737:CDS
TTAAAGTTATTAAAGAAGTT+AGG 0.349767 8.1:-31167196 MS.gene071737:CDS
GGTTGGTGCAAAAGCTTCTA+TGG 0.362512 8.1:-31167057 MS.gene071737:CDS
GGTTCCTGGAATGGGAATAA+GGG 0.368867 8.1:-31167345 MS.gene071737:CDS
GCGTTTGATGTGAAGCTTGA+TGG 0.371046 8.1:-31167239 MS.gene071737:CDS
ATTCCCCTTATTCCCATTCC+AGG 0.371516 8.1:+31167341 None:intergenic
GGTGTAGGTGGAGGGAAAGG+GGG 0.396694 8.1:-31167311 MS.gene071737:CDS
ATTATGATGTTGATGGTTCC+TGG 0.398854 8.1:-31167359 MS.gene071737:CDS
GCTTCCGATTATGATGTTGA+TGG 0.408175 8.1:-31167366 MS.gene071737:CDS
AGAGGTGTAGGTGGAGGGAA+AGG 0.431861 8.1:-31167314 MS.gene071737:CDS
GTTGAAGGAAGCTAAGGATT+TGG 0.435359 8.1:-31167156 MS.gene071737:CDS
GGAACCATCAACATCATAAT+CGG 0.459221 8.1:+31167362 None:intergenic
GAGGTGTAGGTGGAGGGAAA+GGG 0.479166 8.1:-31167313 MS.gene071737:CDS
ATGCCGAGAGGTGTAGGTGG+AGG 0.492930 8.1:-31167320 MS.gene071737:CDS
GATGTTGATGGTTCCTGGAA+TGG 0.504794 8.1:-31167354 MS.gene071737:CDS
ATTGCTAAGTTAAAGGAGGT+TGG 0.508274 8.1:-31167074 MS.gene071737:CDS
TTTGGGGTTGAAGGAAGCTA+AGG 0.515112 8.1:-31167162 MS.gene071737:CDS
TGTGACGAAAGAAGAGGCCG+AGG 0.536944 8.1:-31167102 MS.gene071737:CDS
ATGTTGATGGTTCCTGGAAT+GGG 0.541612 8.1:-31167353 MS.gene071737:CDS
AGGGGAATGCCGAGAGGTGT+AGG 0.541839 8.1:-31167326 MS.gene071737:CDS
GAAGGAAGCTAAGGATTTGG+TGG 0.548600 8.1:-31167153 MS.gene071737:CDS
TAACTTAGCAATAATCCCCT+CGG 0.571218 8.1:+31167085 None:intergenic
GGGTGAGGTGAAGAAAGTTG+AGG 0.571665 8.1:-31167270 MS.gene071737:CDS
TTCCCTCCACCTACACCTCT+CGG 0.576289 8.1:+31167317 None:intergenic
GATTATTGCTAAGTTAAAGG+AGG 0.580906 8.1:-31167078 MS.gene071737:CDS
TGCCGAGAGGTGTAGGTGGA+GGG 0.581642 8.1:-31167319 MS.gene071737:CDS
GGAATGCCGAGAGGTGTAGG+TGG 0.612707 8.1:-31167323 MS.gene071737:CDS
ACACCTTTCTTCAAAAGCGT+AGG 0.629486 8.1:+31167122 None:intergenic
GTGACGAAAGAAGAGGCCGA+GGG 0.636034 8.1:-31167101 MS.gene071737:CDS
GTTCCTGGAATGGGAATAAG+GGG 0.637463 8.1:-31167344 MS.gene071737:CDS
GAAAGGTGTGACGAAAGAAG+AGG 0.651585 8.1:-31167108 MS.gene071737:CDS
TGACGAAAGAAGAGGCCGAG+GGG 0.703464 8.1:-31167100 MS.gene071737:CDS
GGAATAAGGGGAATGCCGAG+AGG 0.729602 8.1:-31167332 MS.gene071737:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAGTTATTAAAGAAGTT+AGG - chr8.1:31167266-31167285 MS.gene071737:CDS 15.0%
GATTATTGCTAAGTTAAAGG+AGG - chr8.1:31167384-31167403 MS.gene071737:CDS 30.0%
! AGTTAGGAGTTTTACTAGTT+TGG - chr8.1:31167282-31167301 MS.gene071737:CDS 30.0%
!! GTTAGGAGTTTTACTAGTTT+GGG - chr8.1:31167283-31167302 MS.gene071737:CDS 30.0%
!! TTAGGAGTTTTACTAGTTTG+GGG - chr8.1:31167284-31167303 MS.gene071737:CDS 30.0%
ATTGCTAAGTTAAAGGAGGT+TGG - chr8.1:31167388-31167407 MS.gene071737:CDS 35.0%
GAAGTAATGCGAGAGAAAAA+GGG - chr8.1:31167064-31167083 MS.gene071737:CDS 35.0%
GGAACCATCAACATCATAAT+CGG + chr8.1:31167103-31167122 None:intergenic 35.0%
GGGGATTATTGCTAAGTTAA+AGG - chr8.1:31167381-31167400 MS.gene071737:CDS 35.0%
TAACTTAGCAATAATCCCCT+CGG + chr8.1:31167380-31167399 None:intergenic 35.0%
TGAAGTAATGCGAGAGAAAA+AGG - chr8.1:31167063-31167082 MS.gene071737:CDS 35.0%
!! ATTATGATGTTGATGGTTCC+TGG - chr8.1:31167103-31167122 MS.gene071737:CDS 35.0%
!! TTTTACTAGTTTGGGGTTGA+AGG - chr8.1:31167291-31167310 MS.gene071737:CDS 35.0%
GCTTCCGATTATGATGTTGA+TGG - chr8.1:31167096-31167115 MS.gene071737:CDS 40.0%
GTTGAAGGAAGCTAAGGATT+TGG - chr8.1:31167306-31167325 MS.gene071737:CDS 40.0%
! ACACCTTTCTTCAAAAGCGT+AGG + chr8.1:31167343-31167362 None:intergenic 40.0%
!! ATGTTGATGGTTCCTGGAAT+GGG - chr8.1:31167109-31167128 MS.gene071737:CDS 40.0%
!!! GAAGCTTGATGGTTTTGATG+CGG - chr8.1:31167234-31167253 MS.gene071737:CDS 40.0%
ATTCCCCTTATTCCCATTCC+AGG + chr8.1:31167124-31167143 None:intergenic 45.0%
GAAGGAAGCTAAGGATTTGG+TGG - chr8.1:31167309-31167328 MS.gene071737:CDS 45.0%
GCGTTTGATGTGAAGCTTGA+TGG - chr8.1:31167223-31167242 MS.gene071737:CDS 45.0%
GGTTCCTGGAATGGGAATAA+GGG - chr8.1:31167117-31167136 MS.gene071737:CDS 45.0%
GTTCCTGGAATGGGAATAAG+GGG - chr8.1:31167118-31167137 MS.gene071737:CDS 45.0%
TGGTTCCTGGAATGGGAATA+AGG - chr8.1:31167116-31167135 MS.gene071737:CDS 45.0%
! GAAAGGTGTGACGAAAGAAG+AGG - chr8.1:31167354-31167373 MS.gene071737:CDS 45.0%
! GGGGTGGTGATAGTGAAAAA+GGG - chr8.1:31167170-31167189 MS.gene071737:CDS 45.0%
! GGGTGGTGATAGTGAAAAAG+GGG - chr8.1:31167171-31167190 MS.gene071737:CDS 45.0%
! GGTGGTGATAGTGAAAAAGG+GGG - chr8.1:31167172-31167191 MS.gene071737:CDS 45.0%
! GGTTGGTGCAAAAGCTTCTA+TGG - chr8.1:31167405-31167424 MS.gene071737:CDS 45.0%
! TGATAGTGAAAAAGGGGGTG+AGG - chr8.1:31167177-31167196 MS.gene071737:CDS 45.0%
! TTTGGGGTTGAAGGAAGCTA+AGG - chr8.1:31167300-31167319 MS.gene071737:CDS 45.0%
!! GATGTTGATGGTTCCTGGAA+TGG - chr8.1:31167108-31167127 MS.gene071737:CDS 45.0%
!! TCGCCTACGCTTTTGAAGAA+AGG - chr8.1:31167337-31167356 MS.gene071737:CDS 45.0%
GGGTGAGGTGAAGAAAGTTG+AGG - chr8.1:31167192-31167211 MS.gene071737:CDS 50.0%
! GGGGGTGGTGATAGTGAAAA+AGG - chr8.1:31167169-31167188 MS.gene071737:CDS 50.0%
AGGTGTAGGTGGAGGGAAAG+GGG - chr8.1:31167150-31167169 MS.gene071737:CDS 55.0%
GAGGTGTAGGTGGAGGGAAA+GGG - chr8.1:31167149-31167168 MS.gene071737:CDS 55.0%
GGAATAAGGGGAATGCCGAG+AGG - chr8.1:31167130-31167149 MS.gene071737:CDS 55.0%
GTGACGAAAGAAGAGGCCGA+GGG - chr8.1:31167361-31167380 MS.gene071737:CDS 55.0%
TGACGAAAGAAGAGGCCGAG+GGG - chr8.1:31167362-31167381 MS.gene071737:CDS 55.0%
TGTGACGAAAGAAGAGGCCG+AGG - chr8.1:31167360-31167379 MS.gene071737:CDS 55.0%
TTCCCTCCACCTACACCTCT+CGG + chr8.1:31167148-31167167 None:intergenic 55.0%
! AGAGGTGTAGGTGGAGGGAA+AGG - chr8.1:31167148-31167167 MS.gene071737:CDS 55.0%
GGTGTAGGTGGAGGGAAAGG+GGG - chr8.1:31167151-31167170 MS.gene071737:CDS 60.0%
! AGGGGAATGCCGAGAGGTGT+AGG - chr8.1:31167136-31167155 MS.gene071737:CDS 60.0%
! ATGCCGAGAGGTGTAGGTGG+AGG - chr8.1:31167142-31167161 MS.gene071737:CDS 60.0%
! GGAATGCCGAGAGGTGTAGG+TGG - chr8.1:31167139-31167158 MS.gene071737:CDS 60.0%
! TGCCGAGAGGTGTAGGTGGA+GGG - chr8.1:31167143-31167162 MS.gene071737:CDS 60.0%
GTAGGTGGAGGGAAAGGGGG+TGG - chr8.1:31167154-31167173 MS.gene071737:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 31167052 31167432 31167052 ID=MS.gene071737
chr8.1 mRNA 31167052 31167432 31167052 ID=MS.gene071737.t1;Parent=MS.gene071737
chr8.1 exon 31167052 31167432 31167052 ID=MS.gene071737.t1.exon1;Parent=MS.gene071737.t1
chr8.1 CDS 31167052 31167432 31167052 ID=cds.MS.gene071737.t1;Parent=MS.gene071737.t1
Gene Sequence

>MS.gene071737

ATGGATCTCGTTGAAGTAATGCGAGAGAAAAAGGGAATCAACGAGCTTCCGATTATGATGTTGATGGTTCCTGGAATGGGAATAAGGGGAATGCCGAGAGGTGTAGGTGGAGGGAAAGGGGGTGGTGATAGTGAAAAAGGGGGTGAGGTGAAGAAAGTTGAGGAGAAAACTGCGTTTGATGTGAAGCTTGATGGTTTTGATGCGGCGTCAAAGATTAAAGTTATTAAAGAAGTTAGGAGTTTTACTAGTTTGGGGTTGAAGGAAGCTAAGGATTTGGTGGAGAAATCGCCTACGCTTTTGAAGAAAGGTGTGACGAAAGAAGAGGCCGAGGGGATTATTGCTAAGTTAAAGGAGGTTGGTGCAAAAGCTTCTATGGAATAA

Protein sequence

>MS.gene071737.t1

MDLVEVMREKKGINELPIMMLMVPGMGIRGMPRGVGGGKGGGDSEKGGEVKKVEEKTAFDVKLDGFDAASKIKVIKEVRSFTSLGLKEAKDLVEKSPTLLKKGVTKEEAEGIIAKLKEVGAKASME