Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071796.t1 | KEH32068.1 | 98.7 | 78 | 1 | 0 | 1 | 78 | 317 | 394 | 2.30E-33 | 151 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071796.t1 | Q9C9C0 | 62.8 | 78 | 29 | 0 | 1 | 78 | 335 | 412 | 1.6e-22 | 106.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071796.t1 | A0A072UQG0 | 98.7 | 78 | 1 | 0 | 1 | 78 | 317 | 394 | 1.7e-33 | 151.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050226 | MS.gene071796 | 0.814146 | 1.79E-51 | -1.69E-46 |
| MS.gene050227 | MS.gene071796 | 0.810429 | 1.16E-50 | -1.69E-46 |
| MS.gene050228 | MS.gene071796 | 0.811357 | 7.29E-51 | -1.69E-46 |
| MS.gene050442 | MS.gene071796 | 0.806745 | 7.09E-50 | -1.69E-46 |
| MS.gene050719 | MS.gene071796 | 0.814138 | 1.79E-51 | -1.69E-46 |
| MS.gene050721 | MS.gene071796 | 0.809888 | 1.51E-50 | -1.69E-46 |
| MS.gene057869 | MS.gene071796 | 0.803492 | 3.40E-49 | -1.69E-46 |
| MS.gene059220 | MS.gene071796 | 0.806962 | 6.37E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071796.t1 | MTR_4g112860 | 98.718 | 78 | 1 | 0 | 1 | 78 | 317 | 394 | 1.13e-45 | 150 |
| MS.gene071796.t1 | MTR_4g112860 | 98.718 | 78 | 1 | 0 | 1 | 78 | 317 | 394 | 5.38e-45 | 150 |
| MS.gene071796.t1 | MTR_4g112860 | 98.718 | 78 | 1 | 0 | 1 | 78 | 317 | 394 | 1.23e-44 | 150 |
| MS.gene071796.t1 | MTR_4g112860 | 98.718 | 78 | 1 | 0 | 1 | 78 | 317 | 394 | 1.51e-44 | 151 |
| MS.gene071796.t1 | MTR_4g112820 | 64.103 | 78 | 22 | 1 | 1 | 78 | 232 | 303 | 5.57e-22 | 87.8 |
| MS.gene071796.t1 | MTR_2g058970 | 67.213 | 61 | 20 | 0 | 18 | 78 | 346 | 406 | 3.56e-20 | 82.8 |
| MS.gene071796.t1 | MTR_4g114810 | 57.692 | 78 | 20 | 2 | 1 | 78 | 219 | 283 | 8.44e-17 | 73.2 |
| MS.gene071796.t1 | MTR_4g134260 | 48.718 | 78 | 27 | 2 | 1 | 78 | 155 | 219 | 2.95e-14 | 65.9 |
| MS.gene071796.t1 | MTR_5g025810 | 47.436 | 78 | 29 | 2 | 1 | 78 | 191 | 256 | 9.67e-13 | 61.6 |
| MS.gene071796.t1 | MTR_8g022470 | 49.351 | 77 | 26 | 2 | 1 | 77 | 88 | 151 | 1.14e-11 | 58.5 |
| MS.gene071796.t1 | MTR_5g083100 | 48.052 | 77 | 27 | 2 | 1 | 77 | 78 | 141 | 1.28e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071796.t1 | AT1G73990 | 62.821 | 78 | 29 | 0 | 1 | 78 | 335 | 412 | 2.08e-28 | 106 |
| MS.gene071796.t1 | AT1G73990 | 62.821 | 78 | 29 | 0 | 1 | 78 | 335 | 412 | 2.18e-28 | 106 |
Find 27 sgRNAs with CRISPR-Local
Find 99 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTGCCTGTGGTTGATTTC+AGG | 0.182093 | 8.1:+15427485 | MS.gene071796:CDS |
| AATACTCTGGAGTTAGAAAA+TGG | 0.307647 | 8.1:+15428806 | MS.gene071796:CDS |
| GCCCCTAGCAAAGGTGTTAT+TGG | 0.308519 | 8.1:+15428913 | MS.gene071796:CDS |
| AAATGGACTGTTGGAATATC+AGG | 0.318883 | 8.1:+15428823 | MS.gene071796:CDS |
| CCTTTGCTAGGGGCACTAAA+TGG | 0.322892 | 8.1:-15428904 | None:intergenic |
| ATAGCCGTCATCCGAGCCTC+AGG | 0.428857 | 8.1:+15428859 | MS.gene071796:CDS |
| TAAGTTGAAGGAGAGACTTC+AGG | 0.449062 | 8.1:+15427447 | MS.gene071796:CDS |
| GCAGGTTATCACTAAGTTGA+AGG | 0.459119 | 8.1:+15427435 | None:intergenic |
| GGAGTTAGAAAATGGACTGT+TGG | 0.480337 | 8.1:+15428814 | MS.gene071796:CDS |
| TCTCCAATAACACCTTTGCT+AGG | 0.488100 | 8.1:-15428916 | None:intergenic |
| CTCAAATACAGAAAATACTC+TGG | 0.496655 | 8.1:+15428793 | MS.gene071796:intron |
| CTCCAATAACACCTTTGCTA+GGG | 0.498463 | 8.1:-15428915 | None:intergenic |
| GACGAATGGTCCCTGAGGCT+CGG | 0.537599 | 8.1:-15428870 | None:intergenic |
| CCATTTAGTGCCCCTAGCAA+AGG | 0.580681 | 8.1:+15428904 | MS.gene071796:CDS |
| GGGACCATTCGTCGTACTGA+GGG | 0.584298 | 8.1:+15428880 | MS.gene071796:CDS |
| TCCAATAACACCTTTGCTAG+GGG | 0.584566 | 8.1:-15428914 | None:intergenic |
| GGTCCCTGAGGCTCGGATGA+CGG | 0.593385 | 8.1:-15428863 | None:intergenic |
| GAGAAGATCCGTAGGGTCAG+AGG | 0.601358 | 8.1:+15428949 | MS.gene071796:CDS |
| AGGGACCATTCGTCGTACTG+AGG | 0.602830 | 8.1:+15428879 | MS.gene071796:CDS |
| TAAGTGTACCTCTGACCCTA+CGG | 0.605579 | 8.1:-15428957 | None:intergenic |
| TGGACCCTCAGTACGACGAA+TGG | 0.619771 | 8.1:-15428884 | None:intergenic |
| AGTTCATTGAGAAGATCCGT+AGG | 0.622768 | 8.1:+15428941 | MS.gene071796:CDS |
| TAGCCGTCATCCGAGCCTCA+GGG | 0.632988 | 8.1:+15428860 | MS.gene071796:CDS |
| TGGACTGTTGGAATATCAGG+TGG | 0.648693 | 8.1:+15428826 | MS.gene071796:CDS |
| ATTTACCTGAAATCAACCAC+AGG | 0.649207 | 8.1:-15427490 | None:intergenic |
| GTTCATTGAGAAGATCCGTA+GGG | 0.678464 | 8.1:+15428942 | MS.gene071796:CDS |
| AGTACGACGAATGGTCCCTG+AGG | 0.759893 | 8.1:-15428875 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATATATATATATATATAT+AGG | + | chr8.1:15427585-15427604 | MS.gene071796:intron | 0.0% |
| !! | TATATATATATATATATATA+GGG | + | chr8.1:15427584-15427603 | MS.gene071796:intron | 0.0% |
| !!! | AAAAATAAATATGATTTTGT+AGG | - | chr8.1:15427868-15427887 | None:intergenic | 10.0% |
| !!! | AAAATAAATATGATTTTGTA+GGG | - | chr8.1:15427867-15427886 | None:intergenic | 10.0% |
| !!! | CATATTTTAATATATAAAAC+TGG | - | chr8.1:15428735-15428754 | None:intergenic | 10.0% |
| !! | TATATATATATATCCTTAGT+TGG | + | chr8.1:15428484-15428503 | MS.gene071796:intron | 15.0% |
| !!! | ATAGTTATGTTATTTGATTT+TGG | - | chr8.1:15428581-15428600 | None:intergenic | 15.0% |
| !! | AAATAAAAACTGTTCTAACT+AGG | + | chr8.1:15428653-15428672 | MS.gene071796:intron | 20.0% |
| !! | TCAATTAATCCATGATTATT+TGG | + | chr8.1:15428083-15428102 | MS.gene071796:intron | 20.0% |
| !! | TTTATGCTTAATGAGTTATT+TGG | - | chr8.1:15428758-15428777 | None:intergenic | 20.0% |
| !!! | GAACAGTTTTTATTTAAAAC+AGG | - | chr8.1:15428648-15428667 | None:intergenic | 20.0% |
| ! | AAAACCCTGAATTAAGAAAT+TGG | - | chr8.1:15427978-15427997 | None:intergenic | 25.0% |
| ! | AAACCCTGAATTAAGAAATT+GGG | - | chr8.1:15427977-15427996 | None:intergenic | 25.0% |
| ! | AAATTTACCATTCTTTGAGT+AGG | + | chr8.1:15427509-15427528 | MS.gene071796:intron | 25.0% |
| ! | AATCCCAATTTCTTAATTCA+GGG | + | chr8.1:15427971-15427990 | MS.gene071796:intron | 25.0% |
| ! | ATATAAGTCTGTTAAGAAAC+AGG | - | chr8.1:15428707-15428726 | None:intergenic | 25.0% |
| ! | ATATTTCTCCAAATGAGATT+TGG | - | chr8.1:15428153-15428172 | None:intergenic | 25.0% |
| ! | GACCATTCAATTTAAATCAA+CGG | - | chr8.1:15428347-15428366 | None:intergenic | 25.0% |
| ! | GACTACTAAAAAGAAGAAAA+AGG | - | chr8.1:15428065-15428084 | None:intergenic | 25.0% |
| ! | TAACTAGGAACTATCTAATA+TGG | + | chr8.1:15428668-15428687 | MS.gene071796:intron | 25.0% |
| ! | TTCATTTCTACTATATGCAA+TGG | - | chr8.1:15427796-15427815 | None:intergenic | 25.0% |
| ! | TTGATTTAAATTGAATGGTC+AGG | + | chr8.1:15428347-15428366 | MS.gene071796:intron | 25.0% |
| !! | ACATGAATTTTATCCAACTA+AGG | - | chr8.1:15428500-15428519 | None:intergenic | 25.0% |
| !! | TATTTTCTGTATTTGAGCAA+AGG | - | chr8.1:15428790-15428809 | None:intergenic | 25.0% |
| !! | TCTAGTTTAGAAATCTACAA+TGG | + | chr8.1:15428533-15428552 | MS.gene071796:intron | 25.0% |
| !!! | ATCCGTTGATTTAAATTGAA+TGG | + | chr8.1:15428342-15428361 | MS.gene071796:intron | 25.0% |
| AGATATGAGAAACAGATGTA+AGG | + | chr8.1:15428190-15428209 | MS.gene071796:intron | 30.0% | |
| ATTAAGAAATTGGGATTCCA+TGG | - | chr8.1:15427968-15427987 | None:intergenic | 30.0% | |
| ATTCCATATCCATATAACCA+TGG | + | chr8.1:15427948-15427967 | MS.gene071796:intron | 30.0% | |
| ATTCCATGGTTATATGGATA+TGG | - | chr8.1:15427954-15427973 | None:intergenic | 30.0% | |
| CTCAAATACAGAAAATACTC+TGG | + | chr8.1:15428793-15428812 | MS.gene071796:intron | 30.0% | |
| GAATCCCAATTTCTTAATTC+AGG | + | chr8.1:15427970-15427989 | MS.gene071796:intron | 30.0% | |
| GACCATTCAATTCAAATCAA+CGG | + | chr8.1:15427678-15427697 | MS.gene071796:intron | 30.0% | |
| GTAGATTAACAAGAGAGAAT+CGG | + | chr8.1:15428223-15428242 | MS.gene071796:intron | 30.0% | |
| GTATGTATGTATCAAATGAG+AGG | - | chr8.1:15428405-15428424 | None:intergenic | 30.0% | |
| TTAAAACACAACTTCTAGGT+TGG | - | chr8.1:15428117-15428136 | None:intergenic | 30.0% | |
| TTGTAGGGATTGATATAGAA+AGG | - | chr8.1:15427852-15427871 | None:intergenic | 30.0% | |
| ! | AACCTAGAAGTTGTGTTTTA+AGG | + | chr8.1:15428116-15428135 | MS.gene071796:intron | 30.0% |
| ! | CTATCTAATATGGATTGCTA+TGG | + | chr8.1:15428678-15428697 | MS.gene071796:intron | 30.0% |
| ! | GATTTGGAACCAAATAATCA+TGG | - | chr8.1:15428095-15428114 | None:intergenic | 30.0% |
| !! | AATACTCTGGAGTTAGAAAA+TGG | + | chr8.1:15428806-15428825 | MS.gene071796:CDS | 30.0% |
| ATCGTTGACATAGATGTCTA+TGG | - | chr8.1:15427930-15427949 | None:intergenic | 35.0% | |
| ATTGGGATTCCATGGTTATA+TGG | - | chr8.1:15427960-15427979 | None:intergenic | 35.0% | |
| ATTTACCTGAAATCAACCAC+AGG | - | chr8.1:15427493-15427512 | None:intergenic | 35.0% | |
| CGTTTCTTCAGCATAGATAA+AGG | + | chr8.1:15427538-15427557 | MS.gene071796:intron | 35.0% | |
| CTCCTTAAAACACAACTTCT+AGG | - | chr8.1:15428121-15428140 | None:intergenic | 35.0% | |
| GCACGTGAAAAAAGAAAAAG+AGG | + | chr8.1:15428276-15428295 | MS.gene071796:intron | 35.0% | |
| TTAACAAGAGAGAATCGGAT+TGG | + | chr8.1:15428228-15428247 | MS.gene071796:intron | 35.0% | |
| TTACAGAGCCAAATCTCATT+TGG | + | chr8.1:15428142-15428161 | MS.gene071796:intron | 35.0% | |
| ! | CATCTGTTTCTCATATCTCT+CGG | - | chr8.1:15428188-15428207 | None:intergenic | 35.0% |
| !! | AAATGGACTGTTGGAATATC+AGG | + | chr8.1:15428823-15428842 | MS.gene071796:CDS | 35.0% |
| !! | AGCCGTTGATTTGAATTGAA+TGG | - | chr8.1:15427683-15427702 | None:intergenic | 35.0% |
| AACAGACAAAAAACTGCCTG+TGG | + | chr8.1:15427474-15427493 | MS.gene071796:CDS | 40.0% | |
| AGAGGAGTTCTTATTGTGAG+AGG | + | chr8.1:15427638-15427657 | MS.gene071796:intron | 40.0% | |
| AGTTCATTGAGAAGATCCGT+AGG | + | chr8.1:15428941-15428960 | MS.gene071796:CDS | 40.0% | |
| ATACAGAAGGAAGATGTTGC+AGG | - | chr8.1:15427733-15427752 | None:intergenic | 40.0% | |
| CTCCAATAACACCTTTGCTA+GGG | - | chr8.1:15428918-15428937 | None:intergenic | 40.0% | |
| GACGTAACCTACTCAAAGAA+TGG | - | chr8.1:15427519-15427538 | None:intergenic | 40.0% | |
| GATTGGAGAAGAAACAGACA+TGG | + | chr8.1:15428245-15428264 | MS.gene071796:intron | 40.0% | |
| GGTACAAGTCATGAGAACTA+CGG | - | chr8.1:15428627-15428646 | None:intergenic | 40.0% | |
| GTTCATTGAGAAGATCCGTA+GGG | + | chr8.1:15428942-15428961 | MS.gene071796:CDS | 40.0% | |
| TACTATATGCAATGGCACCA+TGG | - | chr8.1:15427788-15427807 | None:intergenic | 40.0% | |
| TAGGGGATGTATCAAATGAG+AGG | + | chr8.1:15427620-15427639 | MS.gene071796:intron | 40.0% | |
| TATAGAAAGGAGTTCGTCCA+TGG | - | chr8.1:15427839-15427858 | None:intergenic | 40.0% | |
| TCCAATAACACCTTTGCTAG+GGG | - | chr8.1:15428917-15428936 | None:intergenic | 40.0% | |
| TCTCATATCTCTCGGCATAA+AGG | - | chr8.1:15428180-15428199 | None:intergenic | 40.0% | |
| TCTCCAATAACACCTTTGCT+AGG | - | chr8.1:15428919-15428938 | None:intergenic | 40.0% | |
| TCTTCAGCATAGATAAAGGC+TGG | + | chr8.1:15427542-15427561 | MS.gene071796:intron | 40.0% | |
| TTCTTATTGTGAGAGGTGAG+AGG | + | chr8.1:15427645-15427664 | MS.gene071796:intron | 40.0% | |
| ! | CACAACTTCTAGGTTGGATT+TGG | - | chr8.1:15428111-15428130 | None:intergenic | 40.0% |
| ! | TAAGTTGAAGGAGAGACTTC+AGG | + | chr8.1:15427447-15427466 | MS.gene071796:CDS | 40.0% |
| !! | GGAGTTAGAAAATGGACTGT+TGG | + | chr8.1:15428814-15428833 | MS.gene071796:CDS | 40.0% |
| AGAGGAGCTCTTATTGTGAG+AGG | - | chr8.1:15428387-15428406 | None:intergenic | 45.0% | |
| CAATCCTTCGAAACACTCCA+TGG | + | chr8.1:15427819-15427838 | MS.gene071796:intron | 45.0% | |
| CTCTTATTGTGAGAGGTGAG+AGG | - | chr8.1:15428380-15428399 | None:intergenic | 45.0% | |
| TGGACTGTTGGAATATCAGG+TGG | + | chr8.1:15428826-15428845 | MS.gene071796:CDS | 45.0% | |
| TGGAGAAGAAACAGACATGG+AGG | + | chr8.1:15428248-15428267 | MS.gene071796:intron | 45.0% | |
| ! | AACTGCCTGTGGTTGATTTC+AGG | + | chr8.1:15427485-15427504 | MS.gene071796:CDS | 45.0% |
| ! | AATGGCACCATGGTTTGTGA+GGG | - | chr8.1:15427778-15427797 | None:intergenic | 45.0% |
| ! | TGGAGTGTTTCGAAGGATTG+TGG | - | chr8.1:15427819-15427838 | None:intergenic | 45.0% |
| !! | ATATATATATATATATATAG+GGG | + | chr8.1:15427603-15427622 | MS.gene071796:intron | 5.0% |
| CCATTTAGTGCCCCTAGCAA+AGG | + | chr8.1:15428904-15428923 | MS.gene071796:CDS | 50.0% | |
| TGTCCATCCCTCACAAACCA+TGG | + | chr8.1:15427768-15427787 | MS.gene071796:intron | 50.0% | |
| ! | CAATGGCACCATGGTTTGTG+AGG | - | chr8.1:15427779-15427798 | None:intergenic | 50.0% |
| ! | TCGTCCATGGAGTGTTTCGA+AGG | - | chr8.1:15427826-15427845 | None:intergenic | 50.0% |
| !! | CCTTTGCTAGGGGCACTAAA+TGG | - | chr8.1:15428907-15428926 | None:intergenic | 50.0% |
| !! | GCCCCTAGCAAAGGTGTTAT+TGG | + | chr8.1:15428913-15428932 | MS.gene071796:CDS | 50.0% |
| AGGGACCATTCGTCGTACTG+AGG | + | chr8.1:15428879-15428898 | MS.gene071796:CDS | 55.0% | |
| AGTACGACGAATGGTCCCTG+AGG | - | chr8.1:15428878-15428897 | None:intergenic | 55.0% | |
| GAGAAGATCCGTAGGGTCAG+AGG | + | chr8.1:15428949-15428968 | MS.gene071796:CDS | 55.0% | |
| GGGACCATTCGTCGTACTGA+GGG | + | chr8.1:15428880-15428899 | MS.gene071796:CDS | 55.0% | |
| TGGACCCTCAGTACGACGAA+TGG | - | chr8.1:15428887-15428906 | None:intergenic | 55.0% | |
| ! | GCACCATGGTTTGTGAGGGA+TGG | - | chr8.1:15427774-15427793 | None:intergenic | 55.0% |
| ATAGCCGTCATCCGAGCCTC+AGG | + | chr8.1:15428859-15428878 | MS.gene071796:CDS | 60.0% | |
| GACGAATGGTCCCTGAGGCT+CGG | - | chr8.1:15428873-15428892 | None:intergenic | 60.0% | |
| TAGCCGTCATCCGAGCCTCA+GGG | + | chr8.1:15428860-15428879 | MS.gene071796:CDS | 60.0% | |
| TCCTTCTGTATACGCGCGCG+TGG | + | chr8.1:15427742-15427761 | MS.gene071796:intron | 60.0% | |
| GCCACGCGCGCGTATACAGA+AGG | - | chr8.1:15427746-15427765 | None:intergenic | 65.0% | |
| GGTCCCTGAGGCTCGGATGA+CGG | - | chr8.1:15428866-15428885 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 15427439 | 15428970 | 15427439 | ID=MS.gene071796 |
| chr8.1 | mRNA | 15427439 | 15428970 | 15427439 | ID=MS.gene071796.t1;Parent=MS.gene071796 |
| chr8.1 | exon | 15427439 | 15427506 | 15427439 | ID=MS.gene071796.t1.exon1;Parent=MS.gene071796.t1 |
| chr8.1 | CDS | 15427439 | 15427506 | 15427439 | ID=cds.MS.gene071796.t1;Parent=MS.gene071796.t1 |
| chr8.1 | exon | 15428804 | 15428970 | 15428804 | ID=MS.gene071796.t1.exon2;Parent=MS.gene071796.t1 |
| chr8.1 | CDS | 15428804 | 15428970 | 15428804 | ID=cds.MS.gene071796.t1;Parent=MS.gene071796.t1 |
| Gene Sequence |
| Protein sequence |
