AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072553


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072553.t1 MTR_7g112110 96.682 211 7 0 1 211 5 215 1.26e-151 419
MS.gene072553.t1 MTR_7g112120 78.641 206 40 2 1 202 1 206 2.33e-115 328
MS.gene072553.t1 MTR_1g084040 74.020 204 53 0 1 204 3 206 1.46e-105 305
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072553.t1 AT1G56423 66.010 203 69 0 1 203 1 203 9.21e-98 285
MS.gene072553.t1 AT1G56423 66.010 203 69 0 1 203 1 203 1.05e-97 285

Find 53 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATTAAATTGCTTTGATA+TGG 0.171309 7.3:-4356629 MS.gene072553:CDS
TAATGTAAACTTCCCACTTT+TGG 0.220946 7.3:+4356648 None:intergenic
TGTGATGCGGTTGGCCTTAC+GGG 0.286048 7.3:+4356411 None:intergenic
AGAGGAACTGGTACGTTGTT+TGG 0.307326 7.3:-4356313 MS.gene072553:CDS
GAGCAAAGGCTTGGAAGATT+TGG 0.325168 7.3:-4356265 MS.gene072553:CDS
CTGTGATGCGGTTGGCCTTA+CGG 0.349279 7.3:+4356410 None:intergenic
AACTTACGGTGTAGGTTTCA+TGG 0.367784 7.3:-4356290 MS.gene072553:CDS
TTATTAAATTGCTTTGATAT+GGG 0.378126 7.3:-4356628 MS.gene072553:CDS
GGAAGATTTGGTTATCTTGT+TGG 0.387002 7.3:-4356253 MS.gene072553:CDS
TTATCTTGTTGGAAGTCAAT+TGG 0.396840 7.3:-4356242 MS.gene072553:CDS
TTGGAAGTCAATTGGGAAGT+TGG 0.398362 7.3:-4356234 MS.gene072553:CDS
AGTTGGATTGGAAGTAGAAT+AGG 0.408974 7.3:-4356217 MS.gene072553:CDS
TCTTGTTGATGCTGCTTCAC+AGG 0.412869 7.3:-4356500 MS.gene072553:CDS
AATTGCTTTGATATGGGCTC+AGG 0.437174 7.3:-4356622 MS.gene072553:CDS
GTGCATTTCGTGTTGCAGTA+TGG 0.438736 7.3:-4356166 MS.gene072553:CDS
GTCCCATCCGGAGGAGATGA+TGG 0.439322 7.3:+4356384 None:intergenic
TATCTTGTTGGAAGTCAATT+GGG 0.442064 7.3:-4356241 MS.gene072553:CDS
GAGGGATTCCTCAGAGGAAC+TGG 0.444285 7.3:-4356325 MS.gene072553:CDS
GGTACGTTGTTTGGAACTTA+CGG 0.445217 7.3:-4356304 MS.gene072553:CDS
AGTCAATTGGGAAGTTGGAT+TGG 0.450366 7.3:-4356229 MS.gene072553:CDS
ATGGTATATGATGTGGCTAA+TGG 0.468429 7.3:-4356190 MS.gene072553:CDS
TTTGAAGCTGTTTACTATGG+TGG 0.481190 7.3:-4356358 MS.gene072553:CDS
CAATGATGGCAGATACAGAT+AGG 0.486572 7.3:+4357424 None:intergenic
GAGAACGCGTCATCCAAAAG+TGG 0.497930 7.3:-4356661 MS.gene072553:CDS
GTTGGATTGGAAGTAGAATA+GGG 0.502946 7.3:-4356216 MS.gene072553:CDS
ATCATTGTTCTTAGCATCAC+AGG 0.509850 7.3:-4357408 MS.gene072553:intron
CTTGTTGATGCTGCTTCACA+GGG 0.511056 7.3:-4356499 MS.gene072553:CDS
CAGCATCAACAAGAGCAGAT+TGG 0.514551 7.3:+4356509 None:intergenic
TTGTTTGGAACTTACGGTGT+AGG 0.526991 7.3:-4356298 MS.gene072553:CDS
TCCCATCATCTCCTCCGGAT+GGG 0.528548 7.3:-4356386 MS.gene072553:CDS
CGTAAGGCCAACCGCATCAC+AGG 0.530423 7.3:-4356409 MS.gene072553:CDS
ACTTACGGTGTAGGTTTCAT+GGG 0.535716 7.3:-4356289 MS.gene072553:CDS
ACAGGTCCCATCATCTCCTC+CGG 0.536969 7.3:-4356391 MS.gene072553:CDS
GGCGAAATTGGCTACCCGTA+AGG 0.540905 7.3:-4356425 MS.gene072553:CDS
GGCTCAGGAACGCTAGCATG+TGG 0.544815 7.3:-4356607 MS.gene072553:CDS
GTCCCATCATCTCCTCCGGA+TGG 0.557344 7.3:-4356387 MS.gene072553:CDS
TCTGAGGAATCCCTCTGTAA+AGG 0.559119 7.3:+4356333 None:intergenic
CTCAGGAACGCTAGCATGTG+GGG 0.570098 7.3:-4356605 MS.gene072553:CDS
TGGAAGTAGAATAGGGCTCA+TGG 0.576358 7.3:-4356209 MS.gene072553:CDS
TCCCATCCGGAGGAGATGAT+GGG 0.581940 7.3:+4356385 None:intergenic
TCTCCTCTTCTGAAAATCCA+TGG 0.583117 7.3:+4357458 None:intergenic
TATGGTGGTACCTTTACAGA+GGG 0.585211 7.3:-4356343 MS.gene072553:CDS
GCTCAGGAACGCTAGCATGT+GGG 0.607284 7.3:-4356606 MS.gene072553:CDS
TGTGATGCTAAGAACAATGA+TGG 0.627342 7.3:+4357410 None:intergenic
GATGGGACCTGTGATGCGGT+TGG 0.637538 7.3:+4356402 None:intergenic
AGAACGCGTCATCCAAAAGT+GGG 0.637848 7.3:-4356660 MS.gene072553:CDS
AGGGCTCATGGTATATGATG+TGG 0.638967 7.3:-4356197 MS.gene072553:CDS
CTATGGTGGTACCTTTACAG+AGG 0.649919 7.3:-4356344 MS.gene072553:CDS
CTTACGGTGTAGGTTTCATG+GGG 0.659609 7.3:-4356288 MS.gene072553:CDS
TTTACAGAGGGATTCCTCAG+AGG 0.683356 7.3:-4356331 MS.gene072553:CDS
ACAACGTACCAGTTCCTCTG+AGG 0.700531 7.3:+4356317 None:intergenic
AGATGATGGGACCTGTGATG+CGG 0.703866 7.3:+4356398 None:intergenic
TTACGGTGTAGGTTTCATGG+GGG 0.787530 7.3:-4356287 MS.gene072553:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAATTATAATTAATGAAAA+TGG + chr7.3:4356873-4356892 None:intergenic 10.0%
!! ATTATTAAATTGCTTTGATA+TGG - chr7.3:4356971-4356990 MS.gene072553:intron 15.0%
!! ATTTGAATTTATCTATCTAA+TGG + chr7.3:4356712-4356731 None:intergenic 15.0%
!! TTATTAAATTGCTTTGATAT+GGG - chr7.3:4356972-4356991 MS.gene072553:intron 15.0%
!! AGGTTCAAAAATAGTATTAA+TGG + chr7.3:4356233-4356252 None:intergenic 20.0%
!! GGTTCAAAAATAGTATTAAT+GGG + chr7.3:4356232-4356251 None:intergenic 20.0%
!!! AATTGTCTTTATAACGTTTT+GGG - chr7.3:4356281-4356300 MS.gene072553:CDS 20.0%
!!! TAATTGTCTTTATAACGTTT+TGG - chr7.3:4356280-4356299 MS.gene072553:CDS 20.0%
!!! TTTTTTGAAAAAGTGATGTA+AGG + chr7.3:4356791-4356810 None:intergenic 20.0%
! AAATTATTGACCCCAAAAAT+TGG - chr7.3:4356371-4356390 MS.gene072553:CDS 25.0%
!!! AATTTTTGGGGTCAATAATT+TGG + chr7.3:4356372-4356391 None:intergenic 25.0%
!!! TTTTTTGAAGCTGTTTACTA+TGG - chr7.3:4357239-4357258 MS.gene072553:intron 25.0%
AATTAACGGCAGTAATTTAG+GGG - chr7.3:4356307-4356326 MS.gene072553:CDS 30.0%
AATTGTTCCTTACATCAAAG+TGG - chr7.3:4356809-4356828 MS.gene072553:intron 30.0%
CAATTAACGGCAGTAATTTA+GGG - chr7.3:4356306-4356325 MS.gene072553:CDS 30.0%
TATCTTGTTGGAAGTCAATT+GGG - chr7.3:4357359-4357378 MS.gene072553:intron 30.0%
TCAATTAACGGCAGTAATTT+AGG - chr7.3:4356305-4356324 MS.gene072553:CDS 30.0%
TGAATAAGATAAACCTGATC+AGG + chr7.3:4356339-4356358 None:intergenic 30.0%
TGGGTTCTCATATTTAACTA+TGG - chr7.3:4356844-4356863 MS.gene072553:intron 30.0%
TTATCTTGTTGGAAGTCAAT+TGG - chr7.3:4357358-4357377 MS.gene072553:intron 30.0%
! CTATTCTAGCATGGATTTTA+TGG - chr7.3:4356417-4356436 MS.gene072553:CDS 30.0%
! CTTTCTTTTCTATTCTAGCA+TGG - chr7.3:4356408-4356427 MS.gene072553:CDS 30.0%
! TAATGTAAACTTCCCACTTT+TGG + chr7.3:4356955-4356974 None:intergenic 30.0%
!!! AACGTTTTGGGTTCAATTAA+CGG - chr7.3:4356293-4356312 MS.gene072553:CDS 30.0%
AAGCTATCTTGAAGAGTTTG+TGG - chr7.3:4356603-4356622 MS.gene072553:CDS 35.0%
ATCATTGTTCTTAGCATCAC+AGG - chr7.3:4356192-4356211 MS.gene072553:CDS 35.0%
ATGGTATATGATGTGGCTAA+TGG - chr7.3:4357410-4357429 MS.gene072553:CDS 35.0%
CCTGAAACAAGGTAAAAAAG+CGG - chr7.3:4357154-4357173 MS.gene072553:intron 35.0%
TGTGATGCTAAGAACAATGA+TGG + chr7.3:4356193-4356212 None:intergenic 35.0%
TTGAATTGAGTCTGATGTGA+AGG + chr7.3:4356253-4356272 None:intergenic 35.0%
! AGTTGGATTGGAAGTAGAAT+AGG - chr7.3:4357383-4357402 MS.gene072553:intron 35.0%
! ATTTTCAGAAGAGGAGAGTT+CGG - chr7.3:4356148-4356167 MS.gene072553:CDS 35.0%
! GGAAGATTTGGTTATCTTGT+TGG - chr7.3:4357347-4357366 MS.gene072553:intron 35.0%
! GTTGGATTGGAAGTAGAATA+GGG - chr7.3:4357384-4357403 MS.gene072553:intron 35.0%
! TTTGAAGCTGTTTACTATGG+TGG - chr7.3:4357242-4357261 MS.gene072553:intron 35.0%
!!! CGCTTTTTTACCTTGTTTCA+GGG + chr7.3:4357156-4357175 None:intergenic 35.0%
!!! GCATGTTTTTCTGAAGCAAT+TGG + chr7.3:4357134-4357153 None:intergenic 35.0%
AAATATGAGAACCCAGAGCT+AGG + chr7.3:4356839-4356858 None:intergenic 40.0%
AACTTACGGTGTAGGTTTCA+TGG - chr7.3:4357310-4357329 MS.gene072553:intron 40.0%
AATATGAGAACCCAGAGCTA+GGG + chr7.3:4356838-4356857 None:intergenic 40.0%
AATTGCTTTGATATGGGCTC+AGG - chr7.3:4356978-4356997 MS.gene072553:intron 40.0%
ACTTACGGTGTAGGTTTCAT+GGG - chr7.3:4357311-4357330 MS.gene072553:intron 40.0%
AGCTTCAAAAAAGTCCCATC+CGG + chr7.3:4357231-4357250 None:intergenic 40.0%
AGGGAAACCACTTTGATGTA+AGG + chr7.3:4356819-4356838 None:intergenic 40.0%
AGGTAAAAAAGCGGCGAAAT+TGG - chr7.3:4357163-4357182 MS.gene072553:intron 40.0%
AGTCAATTGGGAAGTTGGAT+TGG - chr7.3:4357371-4357390 MS.gene072553:intron 40.0%
CAATGATGGCAGATACAGAT+AGG + chr7.3:4356179-4356198 None:intergenic 40.0%
GAAAAACATGCCCTGAAACA+AGG - chr7.3:4357143-4357162 MS.gene072553:intron 40.0%
TATGGTGGTACCTTTACAGA+GGG - chr7.3:4357257-4357276 MS.gene072553:intron 40.0%
TTGACCCCAAAAATTGGCAT+AGG - chr7.3:4356377-4356396 MS.gene072553:CDS 40.0%
TTGGAAGTCAATTGGGAAGT+TGG - chr7.3:4357366-4357385 MS.gene072553:intron 40.0%
! CGTCTCCTATGCCAATTTTT+GGG + chr7.3:4356385-4356404 None:intergenic 40.0%
! GGTACGTTGTTTGGAACTTA+CGG - chr7.3:4357296-4357315 MS.gene072553:intron 40.0%
! GTCTCCTATGCCAATTTTTG+GGG + chr7.3:4356384-4356403 None:intergenic 40.0%
! TCGTCTCCTATGCCAATTTT+TGG + chr7.3:4356386-4356405 None:intergenic 40.0%
! TTGTTTGGAACTTACGGTGT+AGG - chr7.3:4357302-4357321 MS.gene072553:intron 40.0%
!!! CCGCTTTTTTACCTTGTTTC+AGG + chr7.3:4357157-4357176 None:intergenic 40.0%
AGAACGCGTCATCCAAAAGT+GGG - chr7.3:4356940-4356959 MS.gene072553:intron 45.0%
AGAGGAACTGGTACGTTGTT+TGG - chr7.3:4357287-4357306 MS.gene072553:intron 45.0%
AGGGCTCATGGTATATGATG+TGG - chr7.3:4357403-4357422 MS.gene072553:intron 45.0%
CAGCATCAACAAGAGCAGAT+TGG + chr7.3:4357094-4357113 None:intergenic 45.0%
CCGTTCAGCTGAAAAATGCA+GGG - chr7.3:4356905-4356924 MS.gene072553:intron 45.0%
CTATGGTGGTACCTTTACAG+AGG - chr7.3:4357256-4357275 MS.gene072553:intron 45.0%
CTTACGGTGTAGGTTTCATG+GGG - chr7.3:4357312-4357331 MS.gene072553:intron 45.0%
GAAAAATGCAGGGAGCTTGT+TGG - chr7.3:4356915-4356934 MS.gene072553:intron 45.0%
GAGCAAAGGCTTGGAAGATT+TGG - chr7.3:4357335-4357354 MS.gene072553:intron 45.0%
GTGCATTTCGTGTTGCAGTA+TGG - chr7.3:4357434-4357453 MS.gene072553:CDS 45.0%
TCATTACCACATCACGACAC+AGG + chr7.3:4356752-4356771 None:intergenic 45.0%
TCCGTTCAGCTGAAAAATGC+AGG - chr7.3:4356904-4356923 MS.gene072553:intron 45.0%
TCTGAGGAATCCCTCTGTAA+AGG + chr7.3:4357270-4357289 None:intergenic 45.0%
TGGAAGTAGAATAGGGCTCA+TGG - chr7.3:4357391-4357410 MS.gene072553:intron 45.0%
TTACGGTGTAGGTTTCATGG+GGG - chr7.3:4357313-4357332 MS.gene072553:intron 45.0%
TTCAAAAAAGTCCCATCCGG+AGG + chr7.3:4357228-4357247 None:intergenic 45.0%
TTTACAGAGGGATTCCTCAG+AGG - chr7.3:4357269-4357288 MS.gene072553:intron 45.0%
! AAAGTGGTTTCCCTAGCTCT+GGG - chr7.3:4356825-4356844 MS.gene072553:intron 45.0%
! CTTGTTGATGCTGCTTCACA+GGG - chr7.3:4357101-4357120 MS.gene072553:intron 45.0%
! TCTTGTTGATGCTGCTTCAC+AGG - chr7.3:4357100-4357119 MS.gene072553:intron 45.0%
! TTAGGGGAGTTTTCCTGATC+AGG - chr7.3:4356323-4356342 MS.gene072553:CDS 45.0%
!! CCCTGCATTTTTCAGCTGAA+CGG + chr7.3:4356908-4356927 None:intergenic 45.0%
AACAGACCTGTGTCGTGATG+TGG - chr7.3:4356743-4356762 MS.gene072553:intron 50.0%
ACAACGTACCAGTTCCTCTG+AGG + chr7.3:4357286-4357305 None:intergenic 50.0%
CAAAGTGGTTTCCCTAGCTC+TGG - chr7.3:4356824-4356843 MS.gene072553:intron 50.0%
CATCACGACACAGGTCTGTT+TGG + chr7.3:4356743-4356762 None:intergenic 50.0%
GAGAACGCGTCATCCAAAAG+TGG - chr7.3:4356939-4356958 MS.gene072553:intron 50.0%
TAGGTTTCATGGGGGAGCAA+AGG - chr7.3:4357321-4357340 MS.gene072553:intron 50.0%
!! AGATGATGGGACCTGTGATG+CGG + chr7.3:4357205-4357224 None:intergenic 50.0%
ACAGGTCCCATCATCTCCTC+CGG - chr7.3:4357209-4357228 MS.gene072553:intron 55.0%
CTCAGGAACGCTAGCATGTG+GGG - chr7.3:4356995-4357014 MS.gene072553:intron 55.0%
GAGGGATTCCTCAGAGGAAC+TGG - chr7.3:4357275-4357294 MS.gene072553:intron 55.0%
GCTCAGGAACGCTAGCATGT+GGG - chr7.3:4356994-4357013 MS.gene072553:intron 55.0%
GGCGAAATTGGCTACCCGTA+AGG - chr7.3:4357175-4357194 MS.gene072553:intron 55.0%
TCCCATCATCTCCTCCGGAT+GGG - chr7.3:4357214-4357233 MS.gene072553:intron 55.0%
TCCCATCCGGAGGAGATGAT+GGG + chr7.3:4357218-4357237 None:intergenic 55.0%
TTCATGGGGGAGCAAAGGCT+TGG - chr7.3:4357326-4357345 MS.gene072553:intron 55.0%
!! CTGTGATGCGGTTGGCCTTA+CGG + chr7.3:4357193-4357212 None:intergenic 55.0%
!! TGTGATGCGGTTGGCCTTAC+GGG + chr7.3:4357192-4357211 None:intergenic 55.0%
CGTAAGGCCAACCGCATCAC+AGG - chr7.3:4357191-4357210 MS.gene072553:intron 60.0%
GATGGGACCTGTGATGCGGT+TGG + chr7.3:4357201-4357220 None:intergenic 60.0%
GGCTCAGGAACGCTAGCATG+TGG - chr7.3:4356993-4357012 MS.gene072553:intron 60.0%
GTCCCATCATCTCCTCCGGA+TGG - chr7.3:4357213-4357232 MS.gene072553:intron 60.0%
GTCCCATCCGGAGGAGATGA+TGG + chr7.3:4357219-4357238 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 4356144 4357478 4356144 ID=MS.gene072553
chr7.3 mRNA 4356144 4357478 4356144 ID=MS.gene072553.t1;Parent=MS.gene072553
chr7.3 exon 4357409 4357478 4357409 ID=MS.gene072553.t1.exon1;Parent=MS.gene072553.t1
chr7.3 CDS 4357409 4357478 4357409 ID=cds.MS.gene072553.t1;Parent=MS.gene072553.t1
chr7.3 exon 4356144 4356709 4356144 ID=MS.gene072553.t1.exon2;Parent=MS.gene072553.t1
chr7.3 CDS 4356144 4356709 4356144 ID=cds.MS.gene072553.t1;Parent=MS.gene072553.t1
Gene Sequence

>MS.gene072553

ATGGATTTTCAGAAGAGGAGAGTTCGGTTTCTCCTATCTGTATCTGCCATCATTGTTCTTAGCATCACAGGTATCTATAGCACTTCCCATTAATACTATTTTTGAACCTTCACATCAGACTCAATTCAACACTCAATAATTGTCTTTATAACGTTTTGGGTTCAATTAACGGCAGTAATTTAGGGGAGTTTTCCTGATCAGGTTTATCTTATTCAATGCAAATCTCCAAATTATTGACCCCAAAAATTGGCATAGGAGACGAATCTTTCTTTTCTATTCTAGCATGGATTTTATGGATTGCTTTGAGTTTGTTGTGTGTAAATGTTTTAGACTATATTTTGATAAACAACTTTAGTGTTTATCATATGCTATAACAACTTTAGTGTATAAGCTATTTCTATAACAAATGATGAAAACAAATCAAATTGTTTTCTTATATACTATAAGTTGTTTTCATATAAGCTATCTTGAAGAGTTTGTGGAAATAAGCTGAAACAATGTATGAAAATGTCATAACATGTTTTCATAAGCTCTCTTAAATAGTCTCACAAATGTATAGTTTATACCATTAGATAGATAAATTCAAATAAGTCAATCCAAACAGACCTGTGTCGTGATGTGGTAATGAATTCTTAACTATTGTTCCTTACATCACTTTTTCAAAAAATTGTTCCTTACATCAAAGTGGTTTCCCTAGCTCTGGGTTCTCATATTTAACTATGGAAGCCATTTTCATTAATTATAATTTCTGCTTGTTTGCTCCGTTCAGCTGAAAAATGCAGGGAGCTTGTTGGCGAGAACGCGTCATCCAAAAGTGGGAAGTTTACATTATTAAATTGCTTTGATATGGGCTCAGGAACGCTAGCATGTGGGGTTAAAGAAAGTGTGAAGCTCTATGTTAATAATATTAGATCTGCTCATGTTGAAAAAGCAAGACATGATGCAATCCAATCTGCTCTTGTTGATGCTGCTTCACAGGGTATGTCGCCAATTGCTTCAGAAAAACATGCCCTGAAACAAGGTAAAAAAGCGGCGAAATTGGCTACCCGTAAGGCCAACCGCATCACAGGTCCCATCATCTCCTCCGGATGGGACTTTTTTGAAGCTGTTTACTATGGTGGTACCTTTACAGAGGGATTCCTCAGAGGAACTGGTACGTTGTTTGGAACTTACGGTGTAGGTTTCATGGGGGAGCAAAGGCTTGGAAGATTTGGTTATCTTGTTGGAAGTCAATTGGGAAGTTGGATTGGAAGTAGAATAGGGCTCATGGTATATGATGTGGCTAATGGAGTGCATTTCGTGTTGCAGTATGGAAGTAAAATTCAAGAAACATAA

Protein sequence

>MS.gene072553.t1

MDFQKRRVRFLLSVSAIIVLSITAEKCRELVGENASSKSGKFTLLNCFDMGSGTLACGVKESVKLYVNNIRSAHVEKARHDAIQSALVDAASQGMSPIASEKHALKQGKKAAKLATRKANRITGPIISSGWDFFEAVYYGGTFTEGFLRGTGTLFGTYGVGFMGEQRLGRFGYLVGSQLGSWIGSRIGLMVYDVANGVHFVLQYGSKIQET