AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058146


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058146.t1 MTR_5g008340 91.398 93 8 0 1 93 1 93 8.10e-59 175
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058146.t1 AT2G39725 66.304 92 28 1 1 92 1 89 7.89e-38 122
MS.gene058146.t1 AT2G39725 66.304 92 28 1 1 92 1 89 7.89e-38 122

Find 13 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAGAGTGCAAAGGCTTTC+TGG 0.170483 5.4:+3331342 MS.gene058146:CDS
GCGGCGTTCTTCGTCGTGTT+TGG 0.337693 5.4:-3331418 None:intergenic
CTTAACAACCCTGGTACCAC+TGG 0.414023 5.4:+3331555 MS.gene058146:CDS
GAAGATAACCCAGTGGTACC+AGG 0.475476 5.4:-3331564 None:intergenic
TCCAGAAAGCCTTTGCACTC+TGG 0.484965 5.4:-3331343 None:intergenic
ATTGAATATTTACTTCGTCG+TGG 0.613668 5.4:+3331513 MS.gene058146:CDS
AAGATAACCCAGTGGTACCA+GGG 0.621203 5.4:-3331563 None:intergenic
ATACTAAAGATTCTATGTTG+CGG 0.622444 5.4:-3331437 None:intergenic
TTAACAACCCTGGTACCACT+GGG 0.624488 5.4:+3331556 MS.gene058146:CDS
CAAGTGCTTAGCTTATACAG+AGG 0.632036 5.4:+3331375 MS.gene058146:CDS
TGGGAGGAACCAGAGTGCAA+AGG 0.649416 5.4:+3331334 MS.gene058146:CDS
CTTGAACAGCTTAACAACCC+TGG 0.674609 5.4:+3331546 MS.gene058146:CDS
TTGCAATGAAGATAACCCAG+TGG 0.758644 5.4:-3331571 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATCAATTTCTTTTGAATTA+CGG - chr5.4:3331476-3331495 None:intergenic 20.0%
! ATACTAAAGATTCTATGTTG+CGG - chr5.4:3331440-3331459 None:intergenic 25.0%
ATTGAATATTTACTTCGTCG+TGG + chr5.4:3331513-3331532 MS.gene058146:CDS 30.0%
CAAGTGCTTAGCTTATACAG+AGG + chr5.4:3331375-3331394 MS.gene058146:CDS 40.0%
TTGCAATGAAGATAACCCAG+TGG - chr5.4:3331574-3331593 None:intergenic 40.0%
AAGATAACCCAGTGGTACCA+GGG - chr5.4:3331566-3331585 None:intergenic 45.0%
CTTGAACAGCTTAACAACCC+TGG + chr5.4:3331546-3331565 MS.gene058146:CDS 45.0%
TTAACAACCCTGGTACCACT+GGG + chr5.4:3331556-3331575 MS.gene058146:CDS 45.0%
ACCAGAGTGCAAAGGCTTTC+TGG + chr5.4:3331342-3331361 MS.gene058146:CDS 50.0%
CTTAACAACCCTGGTACCAC+TGG + chr5.4:3331555-3331574 MS.gene058146:CDS 50.0%
GAAGATAACCCAGTGGTACC+AGG - chr5.4:3331567-3331586 None:intergenic 50.0%
TCCAGAAAGCCTTTGCACTC+TGG - chr5.4:3331346-3331365 None:intergenic 50.0%
TGGGAGGAACCAGAGTGCAA+AGG + chr5.4:3331334-3331353 MS.gene058146:CDS 55.0%
GCGGCGTTCTTCGTCGTGTT+TGG - chr5.4:3331421-3331440 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 3331333 3331614 3331333 ID=MS.gene058146
chr5.4 mRNA 3331333 3331614 3331333 ID=MS.gene058146.t1;Parent=MS.gene058146
chr5.4 exon 3331333 3331614 3331333 ID=MS.gene058146.t1.exon1;Parent=MS.gene058146.t1
chr5.4 CDS 3331333 3331614 3331333 ID=cds.MS.gene058146.t1;Parent=MS.gene058146.t1
Gene Sequence

>MS.gene058146

ATGGGAGGAACCAGAGTGCAAAGGCTTTCTGGAATGCAAAAGCAAGTGCTTAGCTTATACAGAGGATTTCTACGCGCAGCACGTTCCAAACACGACGAAGAACGCCGCAACATAGAATCTTTAGTATCTCAAGAATTTCGCCGTAATTCAAAAGAAATTGATCGAAAGAATTTTCAATACATTGAATATTTACTTCGTCGTGGTCATAAACAGCTTGAACAGCTTAACAACCCTGGTACCACTGGGTTATCTTCATTGCAACTTCATTTATCTAAAGATTAA

Protein sequence

>MS.gene058146.t1

MGGTRVQRLSGMQKQVLSLYRGFLRAARSKHDEERRNIESLVSQEFRRNSKEIDRKNFQYIEYLLRRGHKQLEQLNNPGTTGLSSLQLHLSKD