Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072867.t1 | AES73603.1 | 95 | 383 | 15 | 2 | 1 | 379 | 1 | 383 | 5.20E-187 | 663.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072867.t1 | G7JAG4 | 95.0 | 383 | 15 | 2 | 1 | 379 | 1 | 383 | 3.7e-187 | 663.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052674 | MS.gene072867 | 0.804415 | 2.18E-49 | -1.69E-46 |
| MS.gene052675 | MS.gene072867 | 0.808831 | 2.55E-50 | -1.69E-46 |
| MS.gene052677 | MS.gene072867 | 0.805606 | 1.23E-49 | -1.69E-46 |
| MS.gene060802 | MS.gene072867 | 0.817274 | 3.59E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072867.t1 | MTR_3g106630 | 95.039 | 383 | 15 | 2 | 1 | 379 | 1 | 383 | 0.0 | 721 |
| MS.gene072867.t1 | MTR_4g115690 | 43.548 | 372 | 177 | 6 | 10 | 379 | 20 | 360 | 3.32e-91 | 278 |
| MS.gene072867.t1 | MTR_4g014150 | 34.496 | 258 | 120 | 6 | 36 | 290 | 36 | 247 | 4.59e-38 | 137 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 62 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACCAACATACGTTCTAAA+AGG | 0.193644 | 3.2:-87479819 | None:intergenic |
| GGAATGATTCCTAAGATTAT+TGG | 0.204241 | 3.2:+87480051 | MS.gene072867:CDS |
| TTTGCAATACAAGTGGAAAT+TGG | 0.242240 | 3.2:+87480303 | MS.gene072867:CDS |
| TGAGAGAGATTGTAAAGATT+TGG | 0.261084 | 3.2:-87479499 | None:intergenic |
| TTTGATGGGTCCAACGATTA+AGG | 0.295162 | 3.2:+87479933 | MS.gene072867:CDS |
| AGTCTGAATTTCGGATTCTA+AGG | 0.319480 | 3.2:-87479638 | None:intergenic |
| TTGCAATACAAGTGGAAATT+GGG | 0.326426 | 3.2:+87480304 | MS.gene072867:CDS |
| AACAATAAACCAATAATCTT+AGG | 0.326765 | 3.2:-87480060 | None:intergenic |
| GAGATTAGAGAAAAGAGGTT+AGG | 0.345793 | 3.2:+87480237 | MS.gene072867:CDS |
| AGCTATGGCTATAGCGGATT+TGG | 0.357030 | 3.2:+87479876 | MS.gene072867:CDS |
| GTCTAAATAAGTCTGAATTT+CGG | 0.358799 | 3.2:-87479647 | None:intergenic |
| AGATTAGAGAAAAGAGGTTA+GGG | 0.375202 | 3.2:+87480238 | MS.gene072867:CDS |
| GTGTCTTCGTGTTGCGGCTT+TGG | 0.376492 | 3.2:+87480095 | MS.gene072867:CDS |
| GGCTTCGGAGTGTTCTATAA+AGG | 0.379168 | 3.2:+87479969 | MS.gene072867:CDS |
| CGGTGCTTAGAAGGTTCATC+AGG | 0.395543 | 3.2:-87479322 | None:intergenic |
| AAATCTTGTATGAAATGATT+TGG | 0.410065 | 3.2:-87479600 | None:intergenic |
| GGCTTTGATAGCAATGGCTT+CGG | 0.418058 | 3.2:+87479954 | MS.gene072867:CDS |
| CTGTTAGTGCTGAGATATTG+TGG | 0.420831 | 3.2:+87480418 | MS.gene072867:CDS |
| GTAAGAACACGGTGCTTAGA+AGG | 0.430132 | 3.2:-87479331 | None:intergenic |
| GATAAAATTTCATCAACTAA+AGG | 0.439999 | 3.2:-87480024 | None:intergenic |
| TGTTAGTGCTGAGATATTGT+GGG | 0.445099 | 3.2:+87480419 | MS.gene072867:CDS |
| ATTAGATGAGAAGATAGTTA+TGG | 0.445440 | 3.2:-87479476 | None:intergenic |
| TAAAACCTTGATCGAAAACT+TGG | 0.445674 | 3.2:+87479537 | MS.gene072867:CDS |
| ACAGAACAACGAGGATGAAC+AGG | 0.447175 | 3.2:+87479708 | MS.gene072867:CDS |
| CAACGAGGATGAACAGGTTA+AGG | 0.466181 | 3.2:+87479714 | MS.gene072867:CDS |
| ATCTTTGGAAGCTTGTTTGA+GGG | 0.467224 | 3.2:-87479365 | None:intergenic |
| TTGAACCCTTTACTTCGAAA+AGG | 0.468750 | 3.2:+87479839 | MS.gene072867:CDS |
| GATCTTTGGAAGCTTGTTTG+AGG | 0.477620 | 3.2:-87479366 | None:intergenic |
| TAGAGAAAAGAGGTTAGGGG+TGG | 0.485970 | 3.2:+87480242 | MS.gene072867:CDS |
| ATGAAATGATTTGGCGTTGA+AGG | 0.487135 | 3.2:-87479591 | None:intergenic |
| AAGCTCTAAAAGAGCTTCAA+TGG | 0.491502 | 3.2:-87479443 | None:intergenic |
| TGAACCCTTTACTTCGAAAA+GGG | 0.497904 | 3.2:+87479840 | MS.gene072867:CDS |
| TGGAAGAGATTAGAGAAAAG+AGG | 0.504380 | 3.2:+87480232 | MS.gene072867:CDS |
| GTTTGAACAGAGGTTATTGT+CGG | 0.514496 | 3.2:+87479747 | MS.gene072867:CDS |
| AATGATTTGGCGTTGAAGGA+TGG | 0.515159 | 3.2:-87479587 | None:intergenic |
| TTCGAGATTTGCAATACAAG+TGG | 0.518226 | 3.2:+87480296 | MS.gene072867:CDS |
| AACCAACATACGTTCTAAAA+GGG | 0.519907 | 3.2:-87479818 | None:intergenic |
| GAAGAGAAAAGTGAAAGGGT+TGG | 0.521905 | 3.2:-87479401 | None:intergenic |
| GCTATCAAAGCCTTAATCGT+TGG | 0.522227 | 3.2:-87479943 | None:intergenic |
| AAAAGAGGATTTATGTGATG+TGG | 0.525491 | 3.2:+87480212 | MS.gene072867:CDS |
| TGATTTGTGTCTTCGTGTTG+CGG | 0.528822 | 3.2:+87480089 | MS.gene072867:CDS |
| AGCAATGTTACAGCAAGAGT+TGG | 0.539505 | 3.2:+87480164 | MS.gene072867:CDS |
| GAAATTGGAGATACTGAGAT+TGG | 0.551471 | 3.2:+87480359 | MS.gene072867:CDS |
| GTTCTTGCAGTGTCTTCACA+AGG | 0.552523 | 3.2:-87479687 | None:intergenic |
| GGAGATACTGAGATTGGTGA+AGG | 0.553572 | 3.2:+87480365 | MS.gene072867:CDS |
| TTTGAACAGAGGTTATTGTC+GGG | 0.555301 | 3.2:+87479748 | MS.gene072867:CDS |
| TGCAATACAAGTGGAAATTG+GGG | 0.567079 | 3.2:+87480305 | MS.gene072867:CDS |
| GATTAAGGCTTTGATAGCAA+TGG | 0.569015 | 3.2:+87479948 | MS.gene072867:CDS |
| CAAGGTTTATAATGATTACA+CGG | 0.569226 | 3.2:-87479669 | None:intergenic |
| CTTTGAAGAGAAAAGTGAAA+GGG | 0.585350 | 3.2:-87479405 | None:intergenic |
| AGAATCAGCTATGGCTATAG+CGG | 0.587380 | 3.2:+87479870 | MS.gene072867:CDS |
| ATACAAGTGGAAATTGGGGA+AGG | 0.606082 | 3.2:+87480309 | MS.gene072867:CDS |
| CTCTAAAAGAGCTTCAATGG+CGG | 0.608003 | 3.2:-87479440 | None:intergenic |
| GATACTGAGATTGGTGAAGG+AGG | 0.619602 | 3.2:+87480368 | MS.gene072867:CDS |
| GCTTTGAAGAGAAAAGTGAA+AGG | 0.623593 | 3.2:-87479406 | None:intergenic |
| GGTTCGAGAAGAATCAGCTA+TGG | 0.629018 | 3.2:+87479861 | MS.gene072867:CDS |
| GATTAGAGAAAAGAGGTTAG+GGG | 0.631517 | 3.2:+87480239 | MS.gene072867:CDS |
| GTTAGTGCTGAGATATTGTG+GGG | 0.668901 | 3.2:+87480420 | MS.gene072867:CDS |
| GGTGCTTAGAAGGTTCATCA+GGG | 0.684594 | 3.2:-87479321 | None:intergenic |
| AGTTGAGAAGAGTAAGAACA+CGG | 0.702245 | 3.2:-87479342 | None:intergenic |
| CTATTGTTGAGTTTGAACAG+AGG | 0.712522 | 3.2:+87479737 | MS.gene072867:CDS |
| ACTGCAAGAACAGAACAACG+AGG | 0.856159 | 3.2:+87479699 | MS.gene072867:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATCTTGTATGAAATGATT+TGG | - | chr3.2:87479603-87479622 | None:intergenic | 20.0% |
| !! | AACAATAAACCAATAATCTT+AGG | - | chr3.2:87480063-87480082 | None:intergenic | 20.0% |
| !! | GATAAAATTTCATCAACTAA+AGG | - | chr3.2:87480027-87480046 | None:intergenic | 20.0% |
| !!! | GTTGATGAAATTTTATCTAA+TGG | + | chr3.2:87480030-87480049 | MS.gene072867:CDS | 20.0% |
| !!! | GTTTTTGAACTTTGTTATAT+TGG | + | chr3.2:87480123-87480142 | MS.gene072867:CDS | 20.0% |
| !!! | TCAACAAAAATGTTTTTGTT+GGG | + | chr3.2:87479905-87479924 | MS.gene072867:CDS | 20.0% |
| !!! | TTCAACAAAAATGTTTTTGT+TGG | + | chr3.2:87479904-87479923 | MS.gene072867:CDS | 20.0% |
| ! | ATTAGATGAGAAGATAGTTA+TGG | - | chr3.2:87479479-87479498 | None:intergenic | 25.0% |
| ! | CAAGGTTTATAATGATTACA+CGG | - | chr3.2:87479672-87479691 | None:intergenic | 25.0% |
| ! | GAAAACTTGGAAAAATGTAA+AGG | + | chr3.2:87479550-87479569 | MS.gene072867:CDS | 25.0% |
| ! | GTCTAAATAAGTCTGAATTT+CGG | - | chr3.2:87479650-87479669 | None:intergenic | 25.0% |
| ! | TTGGAAAAATGTAAAGGTTA+TGG | + | chr3.2:87479556-87479575 | MS.gene072867:CDS | 25.0% |
| AAAAGAGGATTTATGTGATG+TGG | + | chr3.2:87480212-87480231 | MS.gene072867:CDS | 30.0% | |
| AGAAAAAAGAGAGGTGAAAT+TGG | + | chr3.2:87480344-87480363 | MS.gene072867:CDS | 30.0% | |
| AGATTAGAGAAAAGAGGTTA+GGG | + | chr3.2:87480238-87480257 | MS.gene072867:CDS | 30.0% | |
| GGAATGATTCCTAAGATTAT+TGG | + | chr3.2:87480051-87480070 | MS.gene072867:CDS | 30.0% | |
| TAAAACCTTGATCGAAAACT+TGG | + | chr3.2:87479537-87479556 | MS.gene072867:CDS | 30.0% | |
| TGAGAGAGATTGTAAAGATT+TGG | - | chr3.2:87479502-87479521 | None:intergenic | 30.0% | |
| TTGCAATACAAGTGGAAATT+GGG | + | chr3.2:87480304-87480323 | MS.gene072867:CDS | 30.0% | |
| TTTGCAATACAAGTGGAAAT+TGG | + | chr3.2:87480303-87480322 | MS.gene072867:CDS | 30.0% | |
| ! | AGTTTTCGATCAAGGTTTTA+AGG | - | chr3.2:87479537-87479556 | None:intergenic | 30.0% |
| ! | CTTTGAAGAGAAAAGTGAAA+GGG | - | chr3.2:87479408-87479427 | None:intergenic | 30.0% |
| ! | GATGATTTTGCAAAGAAAAG+AGG | + | chr3.2:87480197-87480216 | MS.gene072867:CDS | 30.0% |
| ! | TTTTTCCAAGTTTTCGATCA+AGG | - | chr3.2:87479545-87479564 | None:intergenic | 30.0% |
| !! | AACCAACATACGTTCTAAAA+GGG | - | chr3.2:87479821-87479840 | None:intergenic | 30.0% |
| !! | GTTTTCGATCAAGGTTTTAA+GGG | - | chr3.2:87479536-87479555 | None:intergenic | 30.0% |
| !!! | GGTTTTGATTTGAAGATCTT+TGG | - | chr3.2:87479383-87479402 | None:intergenic | 30.0% |
| !!! | TTTGTTGGGTTAGTTTTGAT+GGG | + | chr3.2:87479919-87479938 | MS.gene072867:CDS | 30.0% |
| !!! | TTTTGTTGGGTTAGTTTTGA+TGG | + | chr3.2:87479918-87479937 | MS.gene072867:CDS | 30.0% |
| AGTCTGAATTTCGGATTCTA+AGG | - | chr3.2:87479641-87479660 | None:intergenic | 35.0% | |
| AGTTGAGAAGAGTAAGAACA+CGG | - | chr3.2:87479345-87479364 | None:intergenic | 35.0% | |
| CTATTGTTGAGTTTGAACAG+AGG | + | chr3.2:87479737-87479756 | MS.gene072867:CDS | 35.0% | |
| GAAATTGGAGATACTGAGAT+TGG | + | chr3.2:87480359-87480378 | MS.gene072867:CDS | 35.0% | |
| GAGATTAGAGAAAAGAGGTT+AGG | + | chr3.2:87480237-87480256 | MS.gene072867:CDS | 35.0% | |
| GAGTAGTGAAGAAAAAAGAG+AGG | + | chr3.2:87480335-87480354 | MS.gene072867:CDS | 35.0% | |
| GATTAGAGAAAAGAGGTTAG+GGG | + | chr3.2:87480239-87480258 | MS.gene072867:CDS | 35.0% | |
| GTTTGAACAGAGGTTATTGT+CGG | + | chr3.2:87479747-87479766 | MS.gene072867:CDS | 35.0% | |
| TGAACCCTTTACTTCGAAAA+GGG | + | chr3.2:87479840-87479859 | MS.gene072867:CDS | 35.0% | |
| TGCAATACAAGTGGAAATTG+GGG | + | chr3.2:87480305-87480324 | MS.gene072867:CDS | 35.0% | |
| TGGAAGAGATTAGAGAAAAG+AGG | + | chr3.2:87480232-87480251 | MS.gene072867:CDS | 35.0% | |
| TTCGAGATTTGCAATACAAG+TGG | + | chr3.2:87480296-87480315 | MS.gene072867:CDS | 35.0% | |
| TTGAACCCTTTACTTCGAAA+AGG | + | chr3.2:87479839-87479858 | MS.gene072867:CDS | 35.0% | |
| TTTGAACAGAGGTTATTGTC+GGG | + | chr3.2:87479748-87479767 | MS.gene072867:CDS | 35.0% | |
| ! | ATCTTTGGAAGCTTGTTTGA+GGG | - | chr3.2:87479368-87479387 | None:intergenic | 35.0% |
| ! | ATGAAATGATTTGGCGTTGA+AGG | - | chr3.2:87479594-87479613 | None:intergenic | 35.0% |
| ! | CAACCAACATACGTTCTAAA+AGG | - | chr3.2:87479822-87479841 | None:intergenic | 35.0% |
| ! | GATTAAGGCTTTGATAGCAA+TGG | + | chr3.2:87479948-87479967 | MS.gene072867:CDS | 35.0% |
| ! | TGTTAGTGCTGAGATATTGT+GGG | + | chr3.2:87480419-87480438 | MS.gene072867:CDS | 35.0% |
| !! | AAGCTCTAAAAGAGCTTCAA+TGG | - | chr3.2:87479446-87479465 | None:intergenic | 35.0% |
| !! | GCTTTGAAGAGAAAAGTGAA+AGG | - | chr3.2:87479409-87479428 | None:intergenic | 35.0% |
| !!! | GTTATTGTCGGGTTTTGATT+TGG | + | chr3.2:87479759-87479778 | MS.gene072867:CDS | 35.0% |
| !!! | TACCCTTTTAGAACGTATGT+TGG | + | chr3.2:87479816-87479835 | MS.gene072867:CDS | 35.0% |
| AGAATCAGCTATGGCTATAG+CGG | + | chr3.2:87479870-87479889 | MS.gene072867:CDS | 40.0% | |
| AGCAATGTTACAGCAAGAGT+TGG | + | chr3.2:87480164-87480183 | MS.gene072867:CDS | 40.0% | |
| ATACAAGTGGAAATTGGGGA+AGG | + | chr3.2:87480309-87480328 | MS.gene072867:CDS | 40.0% | |
| GAAGAGAAAAGTGAAAGGGT+TGG | - | chr3.2:87479404-87479423 | None:intergenic | 40.0% | |
| GCTATCAAAGCCTTAATCGT+TGG | - | chr3.2:87479946-87479965 | None:intergenic | 40.0% | |
| ! | AATGATTTGGCGTTGAAGGA+TGG | - | chr3.2:87479590-87479609 | None:intergenic | 40.0% |
| ! | CGAACCCTTTTCGAAGTAAA+GGG | - | chr3.2:87479847-87479866 | None:intergenic | 40.0% |
| ! | CTCTAAAAGAGCTTCAATGG+CGG | - | chr3.2:87479443-87479462 | None:intergenic | 40.0% |
| ! | CTGTTAGTGCTGAGATATTG+TGG | + | chr3.2:87480418-87480437 | MS.gene072867:CDS | 40.0% |
| ! | GATCTTTGGAAGCTTGTTTG+AGG | - | chr3.2:87479369-87479388 | None:intergenic | 40.0% |
| ! | GTTAGTGCTGAGATATTGTG+GGG | + | chr3.2:87480420-87480439 | MS.gene072867:CDS | 40.0% |
| ! | TCGAACCCTTTTCGAAGTAA+AGG | - | chr3.2:87479848-87479867 | None:intergenic | 40.0% |
| ! | TGATTTGTGTCTTCGTGTTG+CGG | + | chr3.2:87480089-87480108 | MS.gene072867:CDS | 40.0% |
| ! | TTCAATGGCGGCTTTTGAAT+CGG | - | chr3.2:87479431-87479450 | None:intergenic | 40.0% |
| ! | TTTGATGGGTCCAACGATTA+AGG | + | chr3.2:87479933-87479952 | MS.gene072867:CDS | 40.0% |
| ACAGAACAACGAGGATGAAC+AGG | + | chr3.2:87479708-87479727 | MS.gene072867:CDS | 45.0% | |
| ACTGCAAGAACAGAACAACG+AGG | + | chr3.2:87479699-87479718 | MS.gene072867:CDS | 45.0% | |
| CAACGAGGATGAACAGGTTA+AGG | + | chr3.2:87479714-87479733 | MS.gene072867:CDS | 45.0% | |
| GATACTGAGATTGGTGAAGG+AGG | + | chr3.2:87480368-87480387 | MS.gene072867:CDS | 45.0% | |
| GGAGATACTGAGATTGGTGA+AGG | + | chr3.2:87480365-87480384 | MS.gene072867:CDS | 45.0% | |
| GGTGCTTAGAAGGTTCATCA+GGG | - | chr3.2:87479324-87479343 | None:intergenic | 45.0% | |
| GGTTCGAGAAGAATCAGCTA+TGG | + | chr3.2:87479861-87479880 | MS.gene072867:CDS | 45.0% | |
| GTAAGAACACGGTGCTTAGA+AGG | - | chr3.2:87479334-87479353 | None:intergenic | 45.0% | |
| GTTCTTGCAGTGTCTTCACA+AGG | - | chr3.2:87479690-87479709 | None:intergenic | 45.0% | |
| TAGAGAAAAGAGGTTAGGGG+TGG | + | chr3.2:87480242-87480261 | MS.gene072867:CDS | 45.0% | |
| ! | GGCTTCGGAGTGTTCTATAA+AGG | + | chr3.2:87479969-87479988 | MS.gene072867:CDS | 45.0% |
| ! | GGCTTTGATAGCAATGGCTT+CGG | + | chr3.2:87479954-87479973 | MS.gene072867:CDS | 45.0% |
| !! | AGCTATGGCTATAGCGGATT+TGG | + | chr3.2:87479876-87479895 | MS.gene072867:CDS | 45.0% |
| CGGTGCTTAGAAGGTTCATC+AGG | - | chr3.2:87479325-87479344 | None:intergenic | 50.0% | |
| GTGTCTTCGTGTTGCGGCTT+TGG | + | chr3.2:87480095-87480114 | MS.gene072867:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 87479316 | 87480455 | 87479316 | ID=MS.gene072867 |
| chr3.2 | mRNA | 87479316 | 87480455 | 87479316 | ID=MS.gene072867.t1;Parent=MS.gene072867 |
| chr3.2 | exon | 87479316 | 87480455 | 87479316 | ID=MS.gene072867.t1.exon1;Parent=MS.gene072867.t1 |
| chr3.2 | CDS | 87479316 | 87480455 | 87479316 | ID=cds.MS.gene072867.t1;Parent=MS.gene072867.t1 |
| Gene Sequence |
| Protein sequence |
