Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073278.t1 | PNY04326.1 | 97.5 | 79 | 2 | 0 | 1 | 79 | 198 | 276 | 1.30E-27 | 132.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073278.t1 | A0A2K3PAF8 | 97.5 | 79 | 2 | 0 | 1 | 79 | 219 | 297 | 9.3e-28 | 132.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049821 | MS.gene073278 | 0.814679 | 1.36E-51 | -1.69E-46 |
| MS.gene050807 | MS.gene073278 | 0.802783 | 4.76E-49 | -1.69E-46 |
| MS.gene051700 | MS.gene073278 | 0.809153 | 2.18E-50 | -1.69E-46 |
| MS.gene051719 | MS.gene073278 | 0.825788 | 3.87E-54 | -1.69E-46 |
| MS.gene052133 | MS.gene073278 | 0.8044 | 2.20E-49 | -1.69E-46 |
| MS.gene052673 | MS.gene073278 | -0.800833 | 1.20E-48 | -1.69E-46 |
| MS.gene058852 | MS.gene073278 | 0.808417 | 3.13E-50 | -1.69E-46 |
| MS.gene060587 | MS.gene073278 | 0.820025 | 8.53E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073278.t1 | MTR_4g127970 | 98.765 | 81 | 1 | 0 | 1 | 81 | 293 | 373 | 3.39e-47 | 154 |
| MS.gene073278.t1 | MTR_4g127970 | 100.000 | 80 | 0 | 0 | 1 | 80 | 293 | 372 | 4.68e-47 | 154 |
| MS.gene073278.t1 | MTR_4g127970 | 100.000 | 72 | 0 | 0 | 1 | 72 | 293 | 364 | 9.33e-41 | 138 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073278.t1 | AT2G18876 | 80.519 | 77 | 15 | 0 | 1 | 77 | 198 | 274 | 1.41e-34 | 119 |
| MS.gene073278.t1 | AT2G18876 | 80.519 | 77 | 15 | 0 | 1 | 77 | 296 | 372 | 9.32e-34 | 119 |
| MS.gene073278.t1 | AT5G57410 | 80.952 | 84 | 13 | 1 | 1 | 81 | 290 | 373 | 2.85e-33 | 118 |
| MS.gene073278.t1 | AT5G57410 | 79.762 | 84 | 14 | 1 | 1 | 81 | 250 | 333 | 4.71e-33 | 116 |
| MS.gene073278.t1 | AT5G57410 | 79.762 | 84 | 14 | 1 | 1 | 81 | 290 | 373 | 7.29e-33 | 117 |
Find 14 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTCTGAGCATCTTGCAAT+TGG | 0.281888 | 8.4:-6596644 | None:intergenic |
| AGCAAAGATGGCTTCTATAA+AGG | 0.358500 | 8.4:+6596509 | MS.gene073278:CDS |
| TAAGAAACCCAAACTCAGAT+AGG | 0.410405 | 8.4:-6597525 | None:intergenic |
| AAGGAGCATTATACAAGAGC+AGG | 0.468589 | 8.4:+6596731 | MS.gene073278:CDS |
| AACATCTTGCAAAGTCTGAC+AGG | 0.478571 | 8.4:+6597199 | MS.gene073278:CDS |
| CAGGGAATCGGCACCGGCTG+AGG | 0.485878 | 8.4:+6597306 | MS.gene073278:intron |
| AGAGAGTCTAAGAGCAAAGA+TGG | 0.492833 | 8.4:+6596497 | MS.gene073278:CDS |
| ATTGCAAGATGCTCAGAAAG+AGG | 0.551306 | 8.4:+6596647 | MS.gene073278:CDS |
| AAGCTCAACTTGTTGAAGCA+AGG | 0.554611 | 8.4:+6596712 | MS.gene073278:CDS |
| CATAAAATACATACCTCAGC+CGG | 0.561677 | 8.4:-6597319 | None:intergenic |
| TTGCAAAGTCTGACAGGCCA+AGG | 0.565306 | 8.4:+6597205 | MS.gene073278:CDS |
| TTGAAATATGAAGGAGCGCA+TGG | 0.571327 | 8.4:+6596620 | MS.gene073278:intron |
| GAAAGAGGCAGAAGTCACTT+CGG | 0.590207 | 8.4:+6596662 | MS.gene073278:CDS |
| CCTCAAACAAAATTGTTGCA+TGG | 0.605571 | 8.4:+6597497 | MS.gene073278:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTATTTTGTAATGATTA+GGG | + | chr8.4:6597474-6597493 | MS.gene073278:intron | 10.0% |
| !!! | TATTTTATTTTGTAATGATT+AGG | + | chr8.4:6597473-6597492 | MS.gene073278:intron | 10.0% |
| !!! | ATTTACTTTTTGAATTTTAC+AGG | + | chr8.4:6597287-6597306 | MS.gene073278:intron | 15.0% |
| !!! | TTTACTTTTTGAATTTTACA+GGG | + | chr8.4:6597288-6597307 | MS.gene073278:intron | 15.0% |
| !! | ATATATGAATTGTTTGTGTT+TGG | + | chr8.4:6596579-6596598 | MS.gene073278:intron | 20.0% |
| !! | CAAATTCACACTATAAAATT+AGG | - | chr8.4:6597404-6597423 | None:intergenic | 20.0% |
| !! | GAAAATAAGATACATGTTTA+GGG | - | chr8.4:6597011-6597030 | None:intergenic | 20.0% |
| !! | TGAAAATAAGATACATGTTT+AGG | - | chr8.4:6597012-6597031 | None:intergenic | 20.0% |
| !!! | AAACTAAATTGCTATTGAAT+TGG | + | chr8.4:6596923-6596942 | MS.gene073278:intron | 20.0% |
| !!! | ATTTTATAGTGTGAATTTGT+TGG | + | chr8.4:6597405-6597424 | MS.gene073278:intron | 20.0% |
| ! | GTGATAATATGTTATATTGC+AGG | + | chr8.4:6597161-6597180 | MS.gene073278:intron | 25.0% |
| ! | TATATGCAAGTACTTAATGA+TGG | - | chr8.4:6596564-6596583 | None:intergenic | 25.0% |
| !! | ATTTTGCATCATAGTAAAAC+AGG | - | chr8.4:6596810-6596829 | None:intergenic | 25.0% |
| !! | CATAAAGAACTAATTTGCAT+TGG | - | chr8.4:6596893-6596912 | None:intergenic | 25.0% |
| !! | TTTTGAATTTTACAGGGAAT+CGG | + | chr8.4:6597294-6597313 | MS.gene073278:intron | 25.0% |
| !!! | ATTGAATTGGTGTTTCTTTT+AGG | + | chr8.4:6596936-6596955 | MS.gene073278:intron | 25.0% |
| AGAAAACACTAAGAATACCT+TGG | - | chr8.4:6597225-6597244 | None:intergenic | 30.0% | |
| GCTTGAAAGTTGAAATATGA+AGG | + | chr8.4:6596611-6596630 | MS.gene073278:intron | 30.0% | |
| TGTTTGTGTTTGGTTGTTAA+CGG | + | chr8.4:6596589-6596608 | MS.gene073278:intron | 30.0% | |
| ! | ACTTATAAGTCCATGTCTTT+TGG | + | chr8.4:6596976-6596995 | MS.gene073278:intron | 30.0% |
| ! | ATCTGATGTTTTATGGTGTT+TGG | + | chr8.4:6596867-6596886 | MS.gene073278:intron | 30.0% |
| ! | GCTATTCATCTGATGTTTTA+TGG | + | chr8.4:6596860-6596879 | MS.gene073278:intron | 30.0% |
| ! | GTTTGTCTGTTTCATGTTTT+GGG | + | chr8.4:6597347-6597366 | MS.gene073278:intron | 30.0% |
| ! | TGTTTGTCTGTTTCATGTTT+TGG | + | chr8.4:6597346-6597365 | MS.gene073278:intron | 30.0% |
| !! | TTATAGTGTGAATTTGTTGG+TGG | + | chr8.4:6597408-6597427 | MS.gene073278:intron | 30.0% |
| AAACAGGATTCTAAATGCCT+TGG | - | chr8.4:6596794-6596813 | None:intergenic | 35.0% | |
| AAGAAAGAAGAAAAAAGCCG+TGG | - | chr8.4:6597058-6597077 | None:intergenic | 35.0% | |
| AGCAAAGATGGCTTCTATAA+AGG | + | chr8.4:6596509-6596528 | MS.gene073278:CDS | 35.0% | |
| CATAAAATACATACCTCAGC+CGG | - | chr8.4:6597322-6597341 | None:intergenic | 35.0% | |
| CCTCAAACAAAATTGTTGCA+TGG | + | chr8.4:6597497-6597516 | MS.gene073278:CDS | 35.0% | |
| TCTCTCCTTTAACAAATCCA+CGG | + | chr8.4:6597038-6597057 | MS.gene073278:intron | 35.0% | |
| TTACTGCAGCAGTACATATT+AGG | - | chr8.4:6597263-6597282 | None:intergenic | 35.0% | |
| ! | ATAAGTCCATGTCTTTTGGT+GGG | + | chr8.4:6596980-6596999 | MS.gene073278:intron | 35.0% |
| ! | TATAAGTCCATGTCTTTTGG+TGG | + | chr8.4:6596979-6596998 | MS.gene073278:intron | 35.0% |
| !! | AAAAGCCGTGGATTTGTTAA+AGG | - | chr8.4:6597046-6597065 | None:intergenic | 35.0% |
| !! | CCATGCAACAATTTTGTTTG+AGG | - | chr8.4:6597500-6597519 | None:intergenic | 35.0% |
| !!! | TCTGTTTCATGTTTTGGGTT+GGG | + | chr8.4:6597352-6597371 | MS.gene073278:intron | 35.0% |
| AACATCTTGCAAAGTCTGAC+AGG | + | chr8.4:6597199-6597218 | MS.gene073278:CDS | 40.0% | |
| AGAGAGTCTAAGAGCAAAGA+TGG | + | chr8.4:6596497-6596516 | MS.gene073278:CDS | 40.0% | |
| ATTGCAACAAGTTAGGTCCA+AGG | + | chr8.4:6596774-6596793 | MS.gene073278:intron | 40.0% | |
| ATTGCAAGATGCTCAGAAAG+AGG | + | chr8.4:6596647-6596666 | MS.gene073278:CDS | 40.0% | |
| CTTTCTGAGCATCTTGCAAT+TGG | - | chr8.4:6596647-6596666 | None:intergenic | 40.0% | |
| TCTCGCAATTGCAACAAGTT+AGG | + | chr8.4:6596767-6596786 | MS.gene073278:intron | 40.0% | |
| ! | AGTGCTGGATCATGCTAATT+TGG | + | chr8.4:6597110-6597129 | MS.gene073278:intron | 40.0% |
| ! | TTGAAATATGAAGGAGCGCA+TGG | + | chr8.4:6596620-6596639 | MS.gene073278:intron | 40.0% |
| !! | AAGCTCAACTTGTTGAAGCA+AGG | + | chr8.4:6596712-6596731 | MS.gene073278:CDS | 40.0% |
| !! | AAGGAGCATTATACAAGAGC+AGG | + | chr8.4:6596731-6596750 | MS.gene073278:CDS | 40.0% |
| !! | AGTGTGAATTTGTTGGTGGT+TGG | + | chr8.4:6597412-6597431 | MS.gene073278:intron | 40.0% |
| !! | GGTTGGTGGAAAAGTATCTT+TGG | + | chr8.4:6597429-6597448 | MS.gene073278:intron | 40.0% |
| !! | GTCTGTTTCATGTTTTGGGT+TGG | + | chr8.4:6597351-6597370 | MS.gene073278:intron | 40.0% |
| GAAAGAGGCAGAAGTCACTT+CGG | + | chr8.4:6596662-6596681 | MS.gene073278:CDS | 45.0% | |
| ! | ATTTTACAGGGAATCGGCAC+CGG | + | chr8.4:6597300-6597319 | MS.gene073278:intron | 45.0% |
| !! | GTGAATTTGTTGGTGGTTGG+TGG | + | chr8.4:6597415-6597434 | MS.gene073278:intron | 45.0% |
| GACAGTACTTTGCTCAGTGC+TGG | + | chr8.4:6597095-6597114 | MS.gene073278:intron | 50.0% | |
| TTGCAAAGTCTGACAGGCCA+AGG | + | chr8.4:6597205-6597224 | MS.gene073278:CDS | 50.0% | |
| !! | GCACTGAGCAAAGTACTGTC+AGG | - | chr8.4:6597095-6597114 | None:intergenic | 50.0% |
| GCTGCACCCACCAAAAGACA+TGG | - | chr8.4:6596989-6597008 | None:intergenic | 55.0% | |
| !! | CAGGGAATCGGCACCGGCTG+AGG | + | chr8.4:6597306-6597325 | MS.gene073278:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 6596477 | 6597533 | 6596477 | ID=MS.gene073278 |
| chr8.4 | mRNA | 6596477 | 6597533 | 6596477 | ID=MS.gene073278.t1;Parent=MS.gene073278 |
| chr8.4 | exon | 6596477 | 6596530 | 6596477 | ID=MS.gene073278.t1.exon1;Parent=MS.gene073278.t1 |
| chr8.4 | CDS | 6596477 | 6596530 | 6596477 | ID=cds.MS.gene073278.t1;Parent=MS.gene073278.t1 |
| chr8.4 | exon | 6596633 | 6596752 | 6596633 | ID=MS.gene073278.t1.exon2;Parent=MS.gene073278.t1 |
| chr8.4 | CDS | 6596633 | 6596752 | 6596633 | ID=cds.MS.gene073278.t1;Parent=MS.gene073278.t1 |
| chr8.4 | exon | 6597183 | 6597226 | 6597183 | ID=MS.gene073278.t1.exon3;Parent=MS.gene073278.t1 |
| chr8.4 | CDS | 6597183 | 6597226 | 6597183 | ID=cds.MS.gene073278.t1;Parent=MS.gene073278.t1 |
| chr8.4 | exon | 6597309 | 6597327 | 6597309 | ID=MS.gene073278.t1.exon4;Parent=MS.gene073278.t1 |
| chr8.4 | CDS | 6597309 | 6597327 | 6597309 | ID=cds.MS.gene073278.t1;Parent=MS.gene073278.t1 |
| chr8.4 | exon | 6597495 | 6597533 | 6597495 | ID=MS.gene073278.t1.exon5;Parent=MS.gene073278.t1 |
| chr8.4 | CDS | 6597495 | 6597533 | 6597495 | ID=cds.MS.gene073278.t1;Parent=MS.gene073278.t1 |
| Gene Sequence |
| Protein sequence |
