Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074107.t1 | XP_003618818.1 | 99.5 | 425 | 2 | 0 | 1 | 425 | 1 | 425 | 3.60E-237 | 830.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074107.t1 | Q8W575 | 61.1 | 427 | 162 | 4 | 1 | 425 | 5 | 429 | 1.9e-138 | 493.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074107.t1 | G7KNL3 | 99.5 | 425 | 2 | 0 | 1 | 425 | 1 | 425 | 2.6e-237 | 830.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene013607 | MS.gene074107 | PPI |
| MS.gene06729 | MS.gene074107 | PPI |
| MS.gene06946 | MS.gene074107 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074107.t1 | MTR_6g023350 | 99.529 | 425 | 2 | 0 | 1 | 425 | 1 | 425 | 0.0 | 877 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074107.t1 | AT5G14250 | 61.124 | 427 | 162 | 4 | 1 | 425 | 5 | 429 | 0.0 | 522 |
| MS.gene074107.t1 | AT5G14250 | 58.235 | 340 | 140 | 2 | 1 | 340 | 5 | 342 | 1.36e-136 | 395 |
Find 89 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTTCTTATGCTTTGCTTGT+TGG | 0.295138 | 6.1:-6923342 | MS.gene074107:CDS |
| TCTTAAATGTTGAATCTTGT+TGG | 0.298235 | 6.1:+6917966 | None:intergenic |
| ATCTCACCATTCCATCCTTC+TGG | 0.329223 | 6.1:+6918942 | None:intergenic |
| CTATATCTCTACTGTTATTA+CGG | 0.329893 | 6.1:-6923251 | MS.gene074107:CDS |
| TCAGAACCCTTGCATTGAAT+TGG | 0.340785 | 6.1:-6919491 | MS.gene074107:intron |
| CAAAACTGGTCTATCCATAT+TGG | 0.367815 | 6.1:-6923309 | MS.gene074107:CDS |
| GGATGGAATGGTGAGATTCT+TGG | 0.373161 | 6.1:-6918936 | MS.gene074107:CDS |
| CACATGCATTTCAGCTTCCT+TGG | 0.394724 | 6.1:+6919091 | None:intergenic |
| AGCGAGGGGAGAAGCATACT+TGG | 0.421227 | 6.1:+6920363 | None:intergenic |
| TATATCTCTACTGTTATTAC+GGG | 0.421454 | 6.1:-6923250 | MS.gene074107:intron |
| CGTAATAACAGTAGAGATAT+AGG | 0.426761 | 6.1:+6923252 | None:intergenic |
| TTGGCAAAATGCTACAAAAC+TGG | 0.432018 | 6.1:-6923323 | MS.gene074107:CDS |
| CTCCAATGTTCACTGCTCTT+CGG | 0.438093 | 6.1:-6923409 | MS.gene074107:CDS |
| CCTTGGATTTGTGCGATCAA+TGG | 0.442532 | 6.1:+6926681 | None:intergenic |
| TTACTTAATGTTCAGAATGA+TGG | 0.450319 | 6.1:-6918712 | MS.gene074107:intron |
| ATTGGAGCCACCCCACGTAT+AGG | 0.459403 | 6.1:+6923425 | None:intergenic |
| GGATCAATGAGTTGAAGAAT+AGG | 0.461517 | 6.1:+6926567 | None:intergenic |
| CTGAATATTCCGCTTATACA+TGG | 0.466470 | 6.1:+6919181 | None:intergenic |
| CAACCTTGGACTGGCTAAGC+AGG | 0.473859 | 6.1:-6919213 | MS.gene074107:CDS |
| CGGAGGAGCTTGATAGACCT+TGG | 0.473958 | 6.1:+6926664 | None:intergenic |
| TTCTCTCATTCGTCATGGAC+AGG | 0.474183 | 6.1:-6920629 | MS.gene074107:intron |
| GCATAAGAAACTCTGCATGC+AGG | 0.480835 | 6.1:+6923354 | None:intergenic |
| AGGATAGAGACGAGATGAAT+CGG | 0.484867 | 6.1:+6926587 | None:intergenic |
| GAGAAAACCGAGAGAATGAA+TGG | 0.484905 | 6.1:+6926542 | None:intergenic |
| GATCCTTCCATTCATTCTCT+CGG | 0.488403 | 6.1:-6926549 | MS.gene074107:CDS |
| GAGCTTCCAGCAGCGTTACT+AGG | 0.489593 | 6.1:+6923447 | None:intergenic |
| TAGCTATGTCTTCGAGAGAA+AGG | 0.490027 | 6.1:+6919135 | None:intergenic |
| ATCTGTTTGCAAGAGGTTGA+AGG | 0.493333 | 6.1:-6923471 | MS.gene074107:CDS |
| AGAAGCATACTTGGGAAGAC+TGG | 0.496098 | 6.1:+6920372 | None:intergenic |
| CATAAGAAACTCTGCATGCA+GGG | 0.503844 | 6.1:+6923355 | None:intergenic |
| TATAGCATTCATCATAGTCA+TGG | 0.508844 | 6.1:+6920684 | None:intergenic |
| CAATGGTTCGAGAGGATCCA+TGG | 0.510795 | 6.1:+6926698 | None:intergenic |
| TGCTGGAAGCTCCTATACGT+GGG | 0.519506 | 6.1:-6923436 | MS.gene074107:CDS |
| AATATGCTCAATCATTTCGC+AGG | 0.519981 | 6.1:+6918892 | None:intergenic |
| CCGAATCTGTTCCACACTGC+AGG | 0.522288 | 6.1:+6924129 | None:intergenic |
| CAGTAGAGATATAGGTCTCT+CGG | 0.522706 | 6.1:+6923260 | None:intergenic |
| AAATCAGCAGCAAGCTGAAG+AGG | 0.523221 | 6.1:-6924182 | MS.gene074107:CDS |
| TTGATAAATCTTGCTATGAT+CGG | 0.524405 | 6.1:+6924154 | None:intergenic |
| GATTACTTACATTATGAAGA+AGG | 0.524623 | 6.1:+6920823 | None:intergenic |
| AAGACGAGCGATGTCGGCGG+AGG | 0.525728 | 6.1:+6926647 | None:intergenic |
| TTGGTTTCTCTCATTCGTCA+TGG | 0.527954 | 6.1:-6920634 | MS.gene074107:CDS |
| TACAGAGGTATGATATGCAT+TGG | 0.528372 | 6.1:-6920873 | MS.gene074107:intron |
| TTAGGATAACAACCTTGGAC+TGG | 0.529794 | 6.1:-6919222 | MS.gene074107:intron |
| TTCCGAAGAGCAGTGAACAT+TGG | 0.535930 | 6.1:+6923407 | None:intergenic |
| AGCTATGTCTTCGAGAGAAA+GGG | 0.537463 | 6.1:+6919136 | None:intergenic |
| CCTGCAGTGTGGAACAGATT+CGG | 0.542212 | 6.1:-6924129 | MS.gene074107:CDS |
| GAAGCGCTTTCAGAAAGCAT+TGG | 0.543315 | 6.1:-6920847 | MS.gene074107:CDS |
| GTCTGGGCCAATTCAATGCA+AGG | 0.543928 | 6.1:+6919484 | None:intergenic |
| TTTCAGATTTCTTTGAGCGA+GGG | 0.544158 | 6.1:+6920348 | None:intergenic |
| TTCATCGGAGTGTTTCAAGA+TGG | 0.544245 | 6.1:+6926620 | None:intergenic |
| GGTGTGAAGACGAGCGATGT+CGG | 0.548178 | 6.1:+6926641 | None:intergenic |
| GCAACAATCAACCAGAAGGA+TGG | 0.554867 | 6.1:-6918953 | MS.gene074107:CDS |
| GCATTTGTCAATGCAAATGC+AGG | 0.557429 | 6.1:-6919430 | MS.gene074107:CDS |
| TAGTTGTATCTGTTTGCAAG+AGG | 0.567352 | 6.1:-6923478 | MS.gene074107:CDS |
| TGAAATGCATGTGCTACAAA+TGG | 0.568753 | 6.1:-6919081 | MS.gene074107:intron |
| GTTTCAGATTTCTTTGAGCG+AGG | 0.575012 | 6.1:+6920347 | None:intergenic |
| CTGCTGGAAGCTCCTATACG+TGG | 0.576435 | 6.1:-6923437 | MS.gene074107:CDS |
| TTCAGATTTCTTTGAGCGAG+GGG | 0.580162 | 6.1:+6920349 | None:intergenic |
| GAAGGACCTAGTAACGCTGC+TGG | 0.584159 | 6.1:-6923453 | MS.gene074107:CDS |
| AAACCGAGAGAATGAATGGA+AGG | 0.587778 | 6.1:+6926546 | None:intergenic |
| AATCAACCAGAAGGATGGAA+TGG | 0.588048 | 6.1:-6918948 | MS.gene074107:CDS |
| TGGAAAAGTTGCAGAAGTAG+AGG | 0.590824 | 6.1:-6919452 | MS.gene074107:CDS |
| ATAGCATTCATCATAGTCAT+GGG | 0.596693 | 6.1:+6920685 | None:intergenic |
| TGGAATGGTGAGATTCTTGG+AGG | 0.596939 | 6.1:-6918933 | MS.gene074107:CDS |
| ATCGGAACGGAGAGTTTCAT+CGG | 0.598675 | 6.1:+6926605 | None:intergenic |
| AGTTCATTTAAATAGTGCCA+AGG | 0.609254 | 6.1:-6919108 | MS.gene074107:CDS |
| GGAAGCTCCTATACGTGGGG+TGG | 0.610466 | 6.1:-6923432 | MS.gene074107:CDS |
| CAACCTGCTTAGCCAGTCCA+AGG | 0.613925 | 6.1:+6919210 | None:intergenic |
| GTCAAAGAAACTAACTGCAA+CGG | 0.614831 | 6.1:-6918685 | MS.gene074107:CDS |
| CCATTGATCGCACAAATCCA+AGG | 0.619652 | 6.1:-6926681 | MS.gene074107:CDS |
| TTGTCAATGCAAATGCAGGA+AGG | 0.627878 | 6.1:-6919426 | MS.gene074107:CDS |
| CAGTGTGGAACAGATTCGGT+TGG | 0.628406 | 6.1:-6924125 | MS.gene074107:CDS |
| ATATGCAACAATCAACCAGA+AGG | 0.635180 | 6.1:-6918957 | MS.gene074107:CDS |
| TTGTATCATCCATGTATAAG+CGG | 0.635665 | 6.1:-6919190 | MS.gene074107:CDS |
| TGTGCGATCAATGGTTCGAG+AGG | 0.637370 | 6.1:+6926690 | None:intergenic |
| GCGAGGGGAGAAGCATACTT+GGG | 0.641248 | 6.1:+6920364 | None:intergenic |
| TCTGATCTACAGATTCAGGA+TGG | 0.643273 | 6.1:-6918986 | MS.gene074107:intron |
| TTGGCCCAGACTTATAACAA+TGG | 0.648340 | 6.1:-6919472 | MS.gene074107:CDS |
| ATAAGAAACTCTGCATGCAG+GGG | 0.649754 | 6.1:+6923356 | None:intergenic |
| TGATCAGTTGTACTTGTCCA+AGG | 0.651932 | 6.1:-6918646 | MS.gene074107:intron |
| GCTGGAAGCTCCTATACGTG+GGG | 0.660249 | 6.1:-6923435 | MS.gene074107:CDS |
| TCTGGGCCAATTCAATGCAA+GGG | 0.662554 | 6.1:+6919485 | None:intergenic |
| AGAGATATAGGTCTCTCGGA+TGG | 0.677401 | 6.1:+6923264 | None:intergenic |
| GTGAAGACGAGCGATGTCGG+CGG | 0.678484 | 6.1:+6926644 | None:intergenic |
| ATTTATCAATGCCTGCAGTG+TGG | 0.685420 | 6.1:-6924140 | MS.gene074107:CDS |
| CAGGGGAGTCAAATGCTCAG+TGG | 0.685717 | 6.1:+6923373 | None:intergenic |
| ATATTGATTCATCAATCCAG+AGG | 0.706848 | 6.1:-6918874 | MS.gene074107:intron |
| AGAGACGAGATGAATCGGAA+CGG | 0.707548 | 6.1:+6926592 | None:intergenic |
| AAATGCAGGAAGGTTCGAAG+CGG | 0.729443 | 6.1:-6919416 | MS.gene074107:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 6917968 | 6926718 | 6917968 | ID=MS.gene074107 |
| chr6.1 | mRNA | 6917968 | 6926718 | 6917968 | ID=MS.gene074107.t1;Parent=MS.gene074107 |
| chr6.1 | exon | 6926534 | 6926718 | 6926534 | ID=MS.gene074107.t1.exon1;Parent=MS.gene074107.t1 |
| chr6.1 | CDS | 6926534 | 6926718 | 6926534 | ID=cds.MS.gene074107.t1;Parent=MS.gene074107.t1 |
| chr6.1 | exon | 6924113 | 6924231 | 6924113 | ID=MS.gene074107.t1.exon2;Parent=MS.gene074107.t1 |
| chr6.1 | CDS | 6924113 | 6924231 | 6924113 | ID=cds.MS.gene074107.t1;Parent=MS.gene074107.t1 |
| chr6.1 | exon | 6923251 | 6923497 | 6923251 | ID=MS.gene074107.t1.exon3;Parent=MS.gene074107.t1 |
| chr6.1 | CDS | 6923251 | 6923497 | 6923251 | ID=cds.MS.gene074107.t1;Parent=MS.gene074107.t1 |
| chr6.1 | exon | 6920833 | 6920890 | 6920833 | ID=MS.gene074107.t1.exon4;Parent=MS.gene074107.t1 |
| chr6.1 | CDS | 6920833 | 6920890 | 6920833 | ID=cds.MS.gene074107.t1;Parent=MS.gene074107.t1 |
| chr6.1 | exon | 6920630 | 6920719 | 6920630 | ID=MS.gene074107.t1.exon5;Parent=MS.gene074107.t1 |
| chr6.1 | CDS | 6920630 | 6920719 | 6920630 | ID=cds.MS.gene074107.t1;Parent=MS.gene074107.t1 |
| chr6.1 | exon | 6920336 | 6920401 | 6920336 | ID=MS.gene074107.t1.exon6;Parent=MS.gene074107.t1 |
| chr6.1 | CDS | 6920336 | 6920401 | 6920336 | ID=cds.MS.gene074107.t1;Parent=MS.gene074107.t1 |
| chr6.1 | exon | 6919417 | 6919509 | 6919417 | ID=MS.gene074107.t1.exon7;Parent=MS.gene074107.t1 |
| chr6.1 | CDS | 6919417 | 6919509 | 6919417 | ID=cds.MS.gene074107.t1;Parent=MS.gene074107.t1 |
| chr6.1 | exon | 6919082 | 6919240 | 6919082 | ID=MS.gene074107.t1.exon8;Parent=MS.gene074107.t1 |
| chr6.1 | CDS | 6919082 | 6919240 | 6919082 | ID=cds.MS.gene074107.t1;Parent=MS.gene074107.t1 |
| chr6.1 | exon | 6918875 | 6918996 | 6918875 | ID=MS.gene074107.t1.exon9;Parent=MS.gene074107.t1 |
| chr6.1 | CDS | 6918875 | 6918996 | 6918875 | ID=cds.MS.gene074107.t1;Parent=MS.gene074107.t1 |
| chr6.1 | exon | 6918647 | 6918719 | 6918647 | ID=MS.gene074107.t1.exon10;Parent=MS.gene074107.t1 |
| chr6.1 | CDS | 6918647 | 6918719 | 6918647 | ID=cds.MS.gene074107.t1;Parent=MS.gene074107.t1 |
| chr6.1 | exon | 6917968 | 6918033 | 6917968 | ID=MS.gene074107.t1.exon11;Parent=MS.gene074107.t1 |
| chr6.1 | CDS | 6917968 | 6918033 | 6917968 | ID=cds.MS.gene074107.t1;Parent=MS.gene074107.t1 |
| Gene Sequence |
| Protein sequence |
