Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013607.t1 | XP_003602225.1 | 96.3 | 508 | 19 | 0 | 1 | 508 | 1 | 508 | 1.30E-273 | 951.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013607.t1 | Q9FG01 | 72.1 | 509 | 136 | 2 | 1 | 508 | 1 | 504 | 3.9e-215 | 748.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013607.t1 | G7J6U4 | 96.3 | 508 | 19 | 0 | 1 | 508 | 1 | 508 | 9.2e-274 | 951.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013607.t1 | MTR_3g091250 | 96.260 | 508 | 19 | 0 | 1 | 508 | 1 | 508 | 0.0 | 1020 |
| MS.gene013607.t1 | MTR_3g091250 | 96.222 | 450 | 17 | 0 | 59 | 508 | 7 | 456 | 0.0 | 867 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013607.t1 | AT5G06160 | 73.870 | 509 | 127 | 2 | 1 | 508 | 1 | 504 | 0.0 | 746 |
Find 117 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTACCTCCTTTAACCTTTC+AGG | 0.130390 | 3.4:-83531484 | None:intergenic |
| ATCACACAGTTTATTCATTT+TGG | 0.141003 | 3.4:-83533179 | None:intergenic |
| TTTAAAAGAAATTGCATTAA+TGG | 0.158382 | 3.4:+83533153 | MS.gene013607:CDS |
| CTTCGATTTCCTCGTATGTT+AGG | 0.196582 | 3.4:-83533388 | None:intergenic |
| CAACTGAGTTCGATGAAAAT+TGG | 0.228110 | 3.4:+83531341 | MS.gene013607:CDS |
| TCGTTGAAGTTCTTTGTATT+TGG | 0.231113 | 3.4:-83535446 | None:intergenic |
| TGTTGGAAGTTGGTTCATTC+TGG | 0.242281 | 3.4:-83528837 | None:intergenic |
| TATGCGGGAACTATAGTTAT+TGG | 0.255362 | 3.4:+83535354 | MS.gene013607:CDS |
| TCATACTCTTCCTCTAAATC+TGG | 0.274594 | 3.4:-83536379 | None:intergenic |
| ATCTAGGCACTTGCTGCATT+GGG | 0.294288 | 3.4:+83532308 | MS.gene013607:intron |
| CAGCACAGTTGAAGAGCTTA+TGG | 0.302002 | 3.4:+83531453 | MS.gene013607:CDS |
| CAGTATGTCAATTCTAAGTT+TGG | 0.342388 | 3.4:+83529924 | MS.gene013607:CDS |
| ATGCGGGAACTATAGTTATT+GGG | 0.353408 | 3.4:+83535355 | MS.gene013607:CDS |
| TGGGCTACTGGAAGGGTATT+GGG | 0.359820 | 3.4:+83531361 | MS.gene013607:CDS |
| TTGGGCTACTGGAAGGGTAT+TGG | 0.366406 | 3.4:+83531360 | MS.gene013607:CDS |
| CATCTAGGCACTTGCTGCAT+TGG | 0.370534 | 3.4:+83532307 | MS.gene013607:intron |
| ATGGGAAGCCTATACCTTAC+TGG | 0.385559 | 3.4:+83534954 | MS.gene013607:CDS |
| CATTGACTCCAGTAGCAGTT+TGG | 0.391236 | 3.4:-83529064 | None:intergenic |
| GCTGTATAAGCTACATGGTC+TGG | 0.393993 | 3.4:+83534976 | MS.gene013607:CDS |
| AGTGGGTCCTGAAAGGTTAA+AGG | 0.394686 | 3.4:+83531477 | MS.gene013607:CDS |
| GGTGTACCTGAATAGTCTTT+TGG | 0.402619 | 3.4:+83530139 | MS.gene013607:CDS |
| CAGCCCCTGGCGTTGTAAAT+CGG | 0.404084 | 3.4:-83536440 | None:intergenic |
| TGCAGGAAGCAAAAGAGCTT+TGG | 0.404374 | 3.4:+83536317 | MS.gene013607:intron |
| GGGATGGGAGAATGTCATTC+AGG | 0.407351 | 3.4:+83531381 | MS.gene013607:CDS |
| GATACTCCAAAAGACTATTC+AGG | 0.407884 | 3.4:-83530145 | None:intergenic |
| TTCAATTCTACAGGCAGTAC+AGG | 0.408760 | 3.4:+83530117 | MS.gene013607:intron |
| TGTATCAGAGTCACCGTGTT+CGG | 0.411672 | 3.4:+83528869 | MS.gene013607:CDS |
| CTCTTTGAGTCTATCATAGA+AGG | 0.414347 | 3.4:-83529096 | None:intergenic |
| CTGATACAAACGGTCCTTGT+TGG | 0.416952 | 3.4:-83528854 | None:intergenic |
| TTCGATTTCCTCGTATGTTA+GGG | 0.417088 | 3.4:-83533387 | None:intergenic |
| AACTGAGTTCGATGAAAATT+GGG | 0.421192 | 3.4:+83531342 | MS.gene013607:CDS |
| TTCGATGAAAATTGGGCTAC+TGG | 0.424132 | 3.4:+83531349 | MS.gene013607:CDS |
| TTATACAGCCAGTAAGGTAT+AGG | 0.426162 | 3.4:-83534962 | None:intergenic |
| CAAGTGGCGCCCAGATTTAG+AGG | 0.442350 | 3.4:+83536369 | MS.gene013607:CDS |
| GCGTCAACAACACGTGCAGC+TGG | 0.446491 | 3.4:-83529391 | None:intergenic |
| CTTGGTGGCCAAACTGCTAC+TGG | 0.455551 | 3.4:+83529056 | MS.gene013607:CDS |
| TGTAGATACGAGAGTATCAT+AGG | 0.463065 | 3.4:+83529362 | MS.gene013607:intron |
| TCAATTGCAGTGTGCTGAGC+AGG | 0.463086 | 3.4:-83531418 | None:intergenic |
| AGATTCTATCAAGATCTTGC+AGG | 0.470792 | 3.4:-83530196 | None:intergenic |
| TACTGGAAGGGTATTGGGAT+GGG | 0.483346 | 3.4:+83531366 | MS.gene013607:CDS |
| CAACGAAATTACGTCAATTG+AGG | 0.486863 | 3.4:+83535463 | MS.gene013607:CDS |
| CTGTATAAGCTACATGGTCT+GGG | 0.495612 | 3.4:+83534977 | MS.gene013607:CDS |
| GCACACACCTCTTGAAATGC+TGG | 0.495845 | 3.4:+83533057 | MS.gene013607:intron |
| ATGAAAATTGGGCTACTGGA+AGG | 0.496968 | 3.4:+83531353 | MS.gene013607:CDS |
| TAATCCCTTAAAATTACCAA+TGG | 0.498141 | 3.4:+83534925 | MS.gene013607:CDS |
| GCTGCATTGGGACTGAAGAC+TGG | 0.498905 | 3.4:+83532320 | MS.gene013607:CDS |
| AATTTAAGTGCGAGATATGC+GGG | 0.502127 | 3.4:+83535339 | MS.gene013607:CDS |
| TAAGCTACATGGTCTGGGTC+AGG | 0.506287 | 3.4:+83534982 | MS.gene013607:CDS |
| GATTCTATCAAGATCTTGCA+GGG | 0.507050 | 3.4:-83530195 | None:intergenic |
| AATCCCTTAAAATTACCAAT+GGG | 0.508135 | 3.4:+83534926 | MS.gene013607:CDS |
| TCGAGCAGCACACGAGGAAG+TGG | 0.508472 | 3.4:+83528783 | MS.gene013607:CDS |
| AGTTGAAGAGCTTATGGAAG+TGG | 0.508524 | 3.4:+83531459 | MS.gene013607:CDS |
| CTTAAAATTACCAATGGGAT+GGG | 0.511486 | 3.4:+83534931 | MS.gene013607:CDS |
| CAACATGGGATGCGATGCCT+TGG | 0.512114 | 3.4:+83535419 | MS.gene013607:CDS |
| GCTACTATCACTTCCACCAC+TGG | 0.515323 | 3.4:+83528901 | MS.gene013607:CDS |
| AGGTATGCAGAATACTCAAT+TGG | 0.515419 | 3.4:-83529951 | None:intergenic |
| AAATTACCAATGGGATGGGA+TGG | 0.518712 | 3.4:+83534935 | MS.gene013607:CDS |
| CATACTCTTCCTCTAAATCT+GGG | 0.521234 | 3.4:-83536378 | None:intergenic |
| AAACGGTCCTTGTTGGAAGT+TGG | 0.522332 | 3.4:-83528847 | None:intergenic |
| CCTTAAAATTACCAATGGGA+TGG | 0.523259 | 3.4:+83534930 | MS.gene013607:CDS |
| TGTAGCTTATACAGCCAGTA+AGG | 0.523342 | 3.4:-83534968 | None:intergenic |
| ATATACCGATTTACAACGCC+AGG | 0.529015 | 3.4:+83536435 | MS.gene013607:CDS |
| GAGAATGTCATTCAGGAGAA+TGG | 0.537200 | 3.4:+83531388 | MS.gene013607:CDS |
| ACACGAGGAAGTGGAGCGAT+TGG | 0.537478 | 3.4:+83528792 | MS.gene013607:CDS |
| TGATAAAGATAATGCAAGGA+AGG | 0.542251 | 3.4:+83529019 | MS.gene013607:CDS |
| AGGAAGTGGAGCGATTGGAG+AGG | 0.543007 | 3.4:+83528797 | MS.gene013607:CDS |
| CTTGCAGGGGTTCTGTCCGA+TGG | 0.546665 | 3.4:-83530181 | None:intergenic |
| GATGAGATTGCTGCACTTGG+TGG | 0.547147 | 3.4:+83529041 | MS.gene013607:CDS |
| GAAGAGTATGAAGACAAAGA+AGG | 0.547972 | 3.4:+83536391 | MS.gene013607:CDS |
| TCTCCGCTAAACTCAATTCG+AGG | 0.550547 | 3.4:-83529448 | None:intergenic |
| GGAAGTGGAGCGATTGGAGA+GGG | 0.550888 | 3.4:+83528798 | MS.gene013607:CDS |
| AAAGAATGGCGTCATCAACA+TGG | 0.552884 | 3.4:+83535404 | MS.gene013607:CDS |
| AAAAGAAATTGCATTAATGG+AGG | 0.560517 | 3.4:+83533156 | MS.gene013607:CDS |
| CTTCTATGATAGACTCAAAG+AGG | 0.564031 | 3.4:+83529097 | MS.gene013607:CDS |
| TGAAAATTGGGCTACTGGAA+GGG | 0.568639 | 3.4:+83531354 | MS.gene013607:CDS |
| TGCGGGAACTATAGTTATTG+GGG | 0.570663 | 3.4:+83535356 | MS.gene013607:CDS |
| GGCTTCCCATCCCATCCCAT+TGG | 0.570952 | 3.4:-83534941 | None:intergenic |
| CACGGTGACTCTGATACAAA+CGG | 0.571196 | 3.4:-83528864 | None:intergenic |
| AATTACCAATGGGATGGGAT+GGG | 0.575961 | 3.4:+83534936 | MS.gene013607:CDS |
| ACTTAGAATTGACATACTGC+TGG | 0.576248 | 3.4:-83529920 | None:intergenic |
| AAGGATGAGATTGCTGCACT+TGG | 0.576506 | 3.4:+83529038 | MS.gene013607:CDS |
| TTATGGAAGTGGGTCCTGAA+AGG | 0.577854 | 3.4:+83531470 | MS.gene013607:CDS |
| GAATTTAAGTGCGAGATATG+CGG | 0.584834 | 3.4:+83535338 | MS.gene013607:CDS |
| CGTCAACAACACGTGCAGCT+GGG | 0.591543 | 3.4:-83529390 | None:intergenic |
| GAACCTCGAATTGAGTTTAG+CGG | 0.593969 | 3.4:+83529445 | MS.gene013607:CDS |
| CGCGCAAAATGAAAACAACC+AGG | 0.595239 | 3.4:+83530003 | MS.gene013607:CDS |
| CTACTGGAAGGGTATTGGGA+TGG | 0.595365 | 3.4:+83531365 | MS.gene013607:CDS |
| TCTTACAAGTCGTCCAGTGG+TGG | 0.600104 | 3.4:-83528914 | None:intergenic |
| TACTGGCTGTATAAGCTACA+TGG | 0.603636 | 3.4:+83534971 | MS.gene013607:CDS |
| TTCTTGTCCAGCATTTCAAG+AGG | 0.604903 | 3.4:-83533064 | None:intergenic |
| TCTTTGTATTTGGTATACCA+AGG | 0.611780 | 3.4:-83535436 | None:intergenic |
| AGAGACAAGGAGTGAACAAG+TGG | 0.612327 | 3.4:+83536353 | MS.gene013607:CDS |
| ATGATGATAAAGATAATGCA+AGG | 0.615572 | 3.4:+83529015 | MS.gene013607:CDS |
| AGCAACTCGAGCAGCACACG+AGG | 0.617294 | 3.4:+83528777 | MS.gene013607:CDS |
| TCAATTCTACAGGCAGTACA+GGG | 0.619788 | 3.4:+83530118 | MS.gene013607:intron |
| ATTGGAGAGGGTAATAGTGA+AGG | 0.622961 | 3.4:+83528810 | MS.gene013607:CDS |
| AAGAATGGCGTCATCAACAT+GGG | 0.625608 | 3.4:+83535405 | MS.gene013607:CDS |
| AATCGAAGCAGAACGTGAGG+AGG | 0.626396 | 3.4:+83533403 | MS.gene013607:CDS |
| GTTGAAGAGCTTATGGAAGT+GGG | 0.627195 | 3.4:+83531460 | MS.gene013607:CDS |
| AAGAAGATACAGCAGAGACA+AGG | 0.630262 | 3.4:+83536340 | MS.gene013607:CDS |
| GTAGCTGCTGCTCCACAACA+TGG | 0.632223 | 3.4:+83533127 | MS.gene013607:CDS |
| GGAAATCGAAGCAGAACGTG+AGG | 0.636554 | 3.4:+83533400 | MS.gene013607:CDS |
| GAATGAACCAACTTCCAACA+AGG | 0.648324 | 3.4:+83528840 | MS.gene013607:CDS |
| GAATCTTACAAGTCGTCCAG+TGG | 0.655081 | 3.4:-83528917 | None:intergenic |
| ACTGTGTGATTTGTTAGACG+AGG | 0.661277 | 3.4:+83533192 | MS.gene013607:CDS |
| TCGAATTGAGTTTAGCGGAG+AGG | 0.670960 | 3.4:+83529450 | MS.gene013607:CDS |
| GCATTGGGACTGAAGACTGG+TGG | 0.671657 | 3.4:+83532323 | MS.gene013607:CDS |
| AGAGAGGCTCTTCCTCACAA+AGG | 0.683965 | 3.4:+83532364 | MS.gene013607:CDS |
| CTGTTCAGCAACGTGCAGAG+AGG | 0.688046 | 3.4:+83532348 | MS.gene013607:CDS |
| TAGCAATCATATTCCGAACA+CGG | 0.688533 | 3.4:-83528882 | None:intergenic |
| ATTCTATCAAGATCTTGCAG+GGG | 0.691153 | 3.4:-83530194 | None:intergenic |
| GGGTCCTGAAAGGTTAAAGG+AGG | 0.691274 | 3.4:+83531480 | MS.gene013607:CDS |
| ATTGCAGGAAGAGACTCAAG+AGG | 0.696019 | 3.4:+83534868 | MS.gene013607:intron |
| TATACCGATTTACAACGCCA+GGG | 0.704758 | 3.4:+83536436 | MS.gene013607:CDS |
| GCGGGAACTATAGTTATTGG+GGG | 0.717346 | 3.4:+83535357 | MS.gene013607:CDS |
| GAAGCAAGCCCTAACATACG+AGG | 0.718372 | 3.4:+83533379 | MS.gene013607:CDS |
| ATACCGATTTACAACGCCAG+GGG | 0.730189 | 3.4:+83536437 | MS.gene013607:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 83528757 | 83536468 | 83528757 | ID=MS.gene013607 |
| chr3.4 | mRNA | 83528757 | 83536468 | 83528757 | ID=MS.gene013607.t1;Parent=MS.gene013607 |
| chr3.4 | exon | 83528757 | 83528930 | 83528757 | ID=MS.gene013607.t1.exon1;Parent=MS.gene013607.t1 |
| chr3.4 | CDS | 83528757 | 83528930 | 83528757 | ID=cds.MS.gene013607.t1;Parent=MS.gene013607.t1 |
| chr3.4 | exon | 83529005 | 83529118 | 83529005 | ID=MS.gene013607.t1.exon2;Parent=MS.gene013607.t1 |
| chr3.4 | CDS | 83529005 | 83529118 | 83529005 | ID=cds.MS.gene013607.t1;Parent=MS.gene013607.t1 |
| chr3.4 | exon | 83529367 | 83529471 | 83529367 | ID=MS.gene013607.t1.exon3;Parent=MS.gene013607.t1 |
| chr3.4 | CDS | 83529367 | 83529471 | 83529367 | ID=cds.MS.gene013607.t1;Parent=MS.gene013607.t1 |
| chr3.4 | exon | 83529882 | 83530024 | 83529882 | ID=MS.gene013607.t1.exon4;Parent=MS.gene013607.t1 |
| chr3.4 | CDS | 83529882 | 83530024 | 83529882 | ID=cds.MS.gene013607.t1;Parent=MS.gene013607.t1 |
| chr3.4 | exon | 83530130 | 83530226 | 83530130 | ID=MS.gene013607.t1.exon5;Parent=MS.gene013607.t1 |
| chr3.4 | CDS | 83530130 | 83530226 | 83530130 | ID=cds.MS.gene013607.t1;Parent=MS.gene013607.t1 |
| chr3.4 | exon | 83531337 | 83531501 | 83531337 | ID=MS.gene013607.t1.exon6;Parent=MS.gene013607.t1 |
| chr3.4 | CDS | 83531337 | 83531501 | 83531337 | ID=cds.MS.gene013607.t1;Parent=MS.gene013607.t1 |
| chr3.4 | exon | 83532314 | 83532385 | 83532314 | ID=MS.gene013607.t1.exon7;Parent=MS.gene013607.t1 |
| chr3.4 | CDS | 83532314 | 83532385 | 83532314 | ID=cds.MS.gene013607.t1;Parent=MS.gene013607.t1 |
| chr3.4 | exon | 83533058 | 83533213 | 83533058 | ID=MS.gene013607.t1.exon8;Parent=MS.gene013607.t1 |
| chr3.4 | CDS | 83533058 | 83533213 | 83533058 | ID=cds.MS.gene013607.t1;Parent=MS.gene013607.t1 |
| chr3.4 | exon | 83533347 | 83533424 | 83533347 | ID=MS.gene013607.t1.exon9;Parent=MS.gene013607.t1 |
| chr3.4 | CDS | 83533347 | 83533424 | 83533347 | ID=cds.MS.gene013607.t1;Parent=MS.gene013607.t1 |
| chr3.4 | exon | 83534875 | 83535003 | 83534875 | ID=MS.gene013607.t1.exon10;Parent=MS.gene013607.t1 |
| chr3.4 | CDS | 83534875 | 83535003 | 83534875 | ID=cds.MS.gene013607.t1;Parent=MS.gene013607.t1 |
| chr3.4 | exon | 83535338 | 83535484 | 83535338 | ID=MS.gene013607.t1.exon11;Parent=MS.gene013607.t1 |
| chr3.4 | CDS | 83535338 | 83535484 | 83535338 | ID=cds.MS.gene013607.t1;Parent=MS.gene013607.t1 |
| chr3.4 | exon | 83536322 | 83536468 | 83536322 | ID=MS.gene013607.t1.exon12;Parent=MS.gene013607.t1 |
| chr3.4 | CDS | 83536322 | 83536468 | 83536322 | ID=cds.MS.gene013607.t1;Parent=MS.gene013607.t1 |
| Gene Sequence |
| Protein sequence |
