AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene074172

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene074172.t1 MTR_6g021950 99.010 404 4 0 1 404 1 404 0.0 832
MS.gene074172.t1 MTR_4g104320 80.366 382 74 1 24 404 26 407 0.0 633
MS.gene074172.t1 MTR_3g075320 75.434 403 99 0 2 404 4 406 0.0 615
MS.gene074172.t1 MTR_3g086310 74.936 391 95 2 14 404 16 403 0.0 583
MS.gene074172.t1 MTR_1g047370 67.624 383 124 0 22 404 68 450 0.0 562
MS.gene074172.t1 MTR_7g059290 66.062 386 131 0 19 404 59 444 0.0 550
MS.gene074172.t1 MTR_3g086320 66.093 407 134 4 1 404 1 406 0.0 532
MS.gene074172.t1 MTR_1g111590 65.416 373 126 1 27 396 35 407 0.0 520
MS.gene074172.t1 MTR_1g062930 62.760 384 136 1 26 402 34 417 0.0 519
MS.gene074172.t1 MTR_7g102940 61.042 403 155 2 1 402 11 412 3.14e-179 507
MS.gene074172.t1 MTR_4g107870 57.487 374 154 3 34 404 63 434 3.75e-160 457
MS.gene074172.t1 MTR_3g075320 74.296 284 73 0 2 285 4 287 2.92e-155 439
MS.gene074172.t1 MTR_5g038510 56.250 368 160 1 38 404 82 449 2.04e-153 441
MS.gene074172.t1 MTR_8g468980 53.367 401 171 4 20 404 55 455 7.47e-151 435
MS.gene074172.t1 MTR_4g132460 60.571 350 134 3 56 404 39 385 1.44e-150 431
MS.gene074172.t1 MTR_5g038490 59.040 354 144 1 52 404 96 449 1.11e-149 432
MS.gene074172.t1 MTR_6g011420 53.299 394 171 3 24 404 60 453 4.59e-146 422
MS.gene074172.t1 MTR_2g023990 58.857 350 140 3 56 404 63 409 1.16e-145 420
MS.gene074172.t1 MTR_7g009390 58.192 354 147 1 52 404 98 451 7.16e-143 414
MS.gene074172.t1 MTR_8g078390 45.480 354 185 6 56 404 42 392 2.65e-98 298
MS.gene074172.t1 MTR_8g078310 43.968 373 199 7 37 404 25 392 3.97e-97 295
MS.gene074172.t1 MTR_3g030900 45.763 354 184 7 56 404 23 373 5.82e-97 294
MS.gene074172.t1 MTR_8g078350 43.968 373 199 7 37 404 25 392 2.69e-91 280
MS.gene074172.t1 MTR_3g070740 65.854 205 57 2 201 404 134 326 3.14e-91 278
MS.gene074172.t1 MTR_8g078280 44.068 354 190 7 56 404 42 392 3.02e-89 275
MS.gene074172.t1 MTR_4g064420 44.253 348 173 7 56 400 42 371 6.06e-89 275
MS.gene074172.t1 MTR_5g019060 42.373 354 196 6 56 404 41 391 7.44e-84 261
MS.gene074172.t1 MTR_5g035840 42.373 354 196 6 56 404 41 391 2.09e-83 260
MS.gene074172.t1 MTR_5g019160 42.090 354 197 6 56 404 41 391 4.75e-83 259
MS.gene074172.t1 MTR_5g019170 42.609 345 190 6 56 395 41 382 1.18e-80 255
MS.gene074172.t1 MTR_5g019090 42.090 354 197 6 56 404 41 391 1.64e-80 253
MS.gene074172.t1 MTR_2g023980 50.584 257 93 6 131 385 1 225 5.49e-73 231
MS.gene074172.t1 MTR_1g069950 43.657 268 147 3 131 396 1 266 2.74e-68 217
MS.gene074172.t1 MTR_6g077900 43.253 289 141 7 89 374 6 274 1.65e-58 192
MS.gene074172.t1 MTR_1g115550 38.356 292 154 7 69 359 9 275 2.41e-49 170
MS.gene074172.t1 MTR_1g067450 36.140 285 158 4 75 359 64 324 1.99e-39 145
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene074172.t1 AT5G63180 72.772 404 107 2 4 404 29 432 0.0 613
MS.gene074172.t1 AT3G27400 72.457 403 105 2 7 404 15 416 0.0 612
MS.gene074172.t1 AT3G27400 72.457 403 105 2 7 404 11 412 0.0 612
MS.gene074172.t1 AT1G67750 73.522 389 101 1 16 404 22 408 0.0 603
MS.gene074172.t1 AT4G24780 72.864 398 106 2 8 404 12 408 0.0 591
MS.gene074172.t1 AT4G24780 72.864 398 106 2 8 404 12 408 0.0 591
MS.gene074172.t1 AT4G13710 66.412 393 128 1 16 404 78 470 0.0 587
MS.gene074172.t1 AT4G13710 66.412 393 128 1 16 404 59 451 0.0 584
MS.gene074172.t1 AT3G07010 68.069 404 122 1 8 404 13 416 0.0 570
MS.gene074172.t1 AT3G07010 70.822 377 110 0 28 404 7 383 0.0 566
MS.gene074172.t1 AT1G04680 67.358 386 122 1 23 404 44 429 0.0 553
MS.gene074172.t1 AT3G24670 68.325 382 121 0 23 404 59 440 0.0 548
MS.gene074172.t1 AT3G24230 65.026 386 130 2 23 404 67 451 0.0 543
MS.gene074172.t1 AT5G48900 64.423 416 134 2 3 404 2 417 0.0 533
MS.gene074172.t1 AT4G13210 68.407 383 120 1 23 404 56 438 0.0 525
MS.gene074172.t1 AT4G13210 68.407 383 120 1 23 404 19 401 0.0 523
MS.gene074172.t1 AT3G53190 62.469 405 136 3 7 396 11 414 0.0 512
MS.gene074172.t1 AT5G04310 62.402 383 131 1 27 396 53 435 2.97e-171 487
MS.gene074172.t1 AT5G04310 62.402 383 131 1 27 396 53 435 6.71e-170 486
MS.gene074172.t1 AT5G04310 62.402 383 131 1 27 396 53 435 6.71e-170 486
MS.gene074172.t1 AT3G54920 59.740 385 131 4 27 396 37 412 2.69e-168 481
MS.gene074172.t1 AT5G55720 56.608 401 159 4 5 404 6 392 1.88e-167 474
MS.gene074172.t1 AT1G14420 54.211 380 166 2 33 404 80 459 4.65e-151 436
MS.gene074172.t1 AT1G11920 58.000 350 144 3 56 404 37 384 3.77e-147 423
MS.gene074172.t1 AT5G15110 55.211 355 157 2 52 404 118 472 2.57e-145 421
MS.gene074172.t1 AT4G22090 57.429 350 145 3 56 404 47 393 4.92e-145 418
MS.gene074172.t1 AT3G01270 56.056 355 154 2 52 404 121 475 5.39e-145 421
MS.gene074172.t1 AT4G22080 56.857 350 147 3 56 404 47 393 2.87e-142 410
MS.gene074172.t1 AT2G02720 53.521 355 159 1 56 404 101 455 5.51e-140 407
MS.gene074172.t1 AT1G30350 51.621 401 161 5 4 404 1 368 1.44e-139 403
MS.gene074172.t1 AT5G09280 47.984 248 127 2 89 335 6 252 2.51e-77 241
MS.gene074172.t1 AT3G55140 39.510 286 147 7 75 359 16 276 1.13e-49 171
MS.gene074172.t1 AT3G09540 36.111 288 158 5 73 359 61 323 4.35e-43 155
MS.gene074172.t1 AT3G55140 39.044 251 132 6 110 359 22 252 9.12e-42 149
MS.gene074172.t1 AT3G09540 35.764 288 155 6 73 359 61 319 4.67e-41 149
MS.gene074172.t1 AT3G09540 35.510 245 134 4 73 317 61 281 1.57e-35 132

Find 86 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTTAATTCACAACATATT+TGG 0.148343 6.1:-5527501 MS.gene074172:CDS
CACCAGAACATAGTGGATTT+TGG 0.165374 6.1:-5527552 MS.gene074172:CDS
TGTCGGTTGTTCTCCCAATT+TGG 0.183698 6.1:+5527928 None:intergenic
GAAGAGGATGAAACAAAATT+TGG 0.190078 6.1:+5528940 None:intergenic
ATGCATACCTTGGCATTCTT+TGG 0.240400 6.1:+5527284 None:intergenic
ATCTACATTGTCACTGATTC+AGG 0.262745 6.1:-5527844 MS.gene074172:CDS
TCAATTAAAACAAGAGCTTT+TGG 0.287323 6.1:-5527725 MS.gene074172:CDS
TCATGACAAAGTTATGCTTT+TGG 0.300199 6.1:-5527383 MS.gene074172:CDS
GCTGATTGTGCAATTGGATT+TGG 0.305034 6.1:-5527898 MS.gene074172:CDS
GTCGGTTGTTCTCCCAATTT+GGG 0.308400 6.1:+5527929 None:intergenic
CAATTCAAGATGAACCATTA+TGG 0.310393 6.1:-5527774 MS.gene074172:CDS
TCCTTGCTAAATCTATTATC+TGG 0.317797 6.1:+5526390 None:intergenic
TACCTTAATGTTCCTGGCTT+AGG 0.318773 6.1:+5527802 None:intergenic
GTTGGAGATGTGACCCAAAT+TGG 0.323275 6.1:-5527942 MS.gene074172:CDS
CAACAGGGAACACAAATATA+AGG 0.331390 6.1:-5527579 MS.gene074172:CDS
GGTTCCATCACTACTACTTC+TGG 0.332285 6.1:-5526201 MS.gene074172:CDS
GCACCATACCTTAATGTTCC+TGG 0.337883 6.1:+5527796 None:intergenic
TTCCCTTGGCTGTTGATTGT+AGG 0.338196 6.1:+5526426 None:intergenic
ACCTTAATGTTCCTGGCTTA+GGG 0.354026 6.1:+5527803 None:intergenic
ACATCTTGTACAACAAGTTC+AGG 0.356255 6.1:+5528907 None:intergenic
AGACTAGCTGATTGTGCAAT+TGG 0.358822 6.1:-5527904 MS.gene074172:CDS
ACCCTAAGCCAGGAACATTA+AGG 0.369613 6.1:-5527804 MS.gene074172:CDS
GTGAATATAATAGCTGGAAT+TGG 0.370547 6.1:-5526338 MS.gene074172:CDS
CACAATCAGCTAGTCTTTGT+CGG 0.381751 6.1:+5527911 None:intergenic
GCAAGACCAGCCTCTCTTGT+TGG 0.384223 6.1:-5526222 MS.gene074172:CDS
GTTCATGATTGTGTTCCAAC+AGG 0.393072 6.1:-5527595 MS.gene074172:CDS
GAGGGTGATTTGTTCTTGAA+TGG 0.394829 6.1:-5526309 MS.gene074172:CDS
AAGAGGATGAAACAAAATTT+GGG 0.396495 6.1:+5528941 None:intergenic
GGCTATTTATCATGTGGAAC+TGG 0.405816 6.1:-5527982 MS.gene074172:CDS
CTTATATTTGTGTTCCCTGT+TGG 0.418044 6.1:+5527580 None:intergenic
GGAGCATTCTTTAGACAAAC+TGG 0.421504 6.1:-5526288 MS.gene074172:CDS
TCCCTTGGCTGTTGATTGTA+GGG 0.426471 6.1:+5526427 None:intergenic
AATTCATACAAGACAATTGA+TGG 0.427271 6.1:-5527700 MS.gene074172:CDS
GATCATATTCAAAAGAGATA+TGG 0.439191 6.1:-5527752 MS.gene074172:CDS
GCAATCCAATTGATGATTGT+TGG 0.439325 6.1:-5527960 MS.gene074172:CDS
CATCTCCAACAATCATCAAT+TGG 0.440002 6.1:+5527955 None:intergenic
GTTAATAATGTTATAATACA+TGG 0.461906 6.1:-5527625 MS.gene074172:CDS
CAGAAAGTGAATATAATAGC+TGG 0.465031 6.1:-5526344 MS.gene074172:CDS
GTCCAAAATCCACTATGTTC+TGG 0.466293 6.1:+5527550 None:intergenic
CTCTAGGTCTAGGAGAAGCT+TGG 0.466344 6.1:-5528004 MS.gene074172:CDS
AACAGGGAACACAAATATAA+GGG 0.474310 6.1:-5527578 MS.gene074172:CDS
GTGCAAGAAATCTGTTCCCT+TGG 0.476596 6.1:+5526412 None:intergenic
GAACAATGATTACACACATT+GGG 0.488170 6.1:-5526478 MS.gene074172:CDS
GCATCAATGTCTCTAGGTCT+AGG 0.489694 6.1:-5528014 MS.gene074172:CDS
AAGAATGAGAAAGTATGAAA+GGG 0.490487 6.1:+5528978 None:intergenic
TTGGAGATGTGACCCAAATT+GGG 0.502512 6.1:-5527941 MS.gene074172:CDS
AAAGAATGAGAAAGTATGAA+AGG 0.502722 6.1:+5528977 None:intergenic
TCTAGGTCTAGGAGAAGCTT+GGG 0.521729 6.1:-5528003 MS.gene074172:CDS
TGGTGTGCTTACTTGCAAGA+AGG 0.524331 6.1:-5526181 MS.gene074172:CDS
AGCTGGAATTGGAGATCAGA+GGG 0.527960 6.1:-5526327 MS.gene074172:CDS
TCTTTGTCTAATTGTCGTGA+TGG 0.533384 6.1:-5527466 MS.gene074172:CDS
ATGGAACCAACAAGAGAGGC+TGG 0.542918 6.1:+5526216 None:intergenic
GTAGGAGCATCAATGTCTCT+AGG 0.545125 6.1:-5528020 MS.gene074172:intron
GGTCTTATTGATGTTATACA+TGG 0.549548 6.1:-5527445 MS.gene074172:CDS
CATTGGGAGATGTATGCAAT+TGG 0.551298 6.1:-5526462 MS.gene074172:CDS
GTGACCTGCATGTCCTTGTC+CGG 0.551806 6.1:+5527340 None:intergenic
TACACCGGACAAGGACATGC+AGG 0.561475 6.1:-5527344 MS.gene074172:CDS
TGGATTTGGAAAAGATGCAA+TGG 0.564287 6.1:-5527884 MS.gene074172:CDS
TACAAGACAATTGATGGAAG+AGG 0.566366 6.1:-5527694 MS.gene074172:CDS
GGTGTGCTTACTTGCAAGAA+GGG 0.567542 6.1:-5526180 MS.gene074172:CDS
ACCAGATAATAGATTTAGCA+AGG 0.568377 6.1:-5526391 MS.gene074172:CDS
TGAACAATGATTACACACAT+TGG 0.569725 6.1:-5526479 MS.gene074172:CDS
AATTCCATCACCATCAGAAA+CGG 0.571243 6.1:+5527528 None:intergenic
AGCTTGGGCTATTTATCATG+TGG 0.571490 6.1:-5527988 MS.gene074172:CDS
AACCCTACAATCAACAGCCA+AGG 0.572050 6.1:-5526429 MS.gene074172:CDS
GGATTTGGAAAAGATGCAAT+GGG 0.573388 6.1:-5527883 MS.gene074172:CDS
AAGCCAGGAACATTAAGGTA+TGG 0.578760 6.1:-5527799 MS.gene074172:CDS
TTCAGGGTCTTGAACTGAAG+AGG 0.579107 6.1:+5528924 None:intergenic
GCTAGTGTTCACATTGCAAA+TGG 0.586396 6.1:-5527670 MS.gene074172:CDS
ACATAGTGATTCTTATACAC+CGG 0.587740 6.1:-5527359 MS.gene074172:CDS
CACACCAGAAGTAGTAGTGA+TGG 0.594623 6.1:+5526197 None:intergenic
AGGGATTCACCAGAACATAG+TGG 0.595458 6.1:-5527559 MS.gene074172:CDS
TGGGAGATGTATGCAATTGG+TGG 0.599619 6.1:-5526459 MS.gene074172:CDS
CATCTTGTACAACAAGTTCA+GGG 0.601679 6.1:+5528908 None:intergenic
TGGACCGTTTCTGATGGTGA+TGG 0.601944 6.1:-5527532 MS.gene074172:CDS
GATGCAGTAAACCCTAAGCC+AGG 0.603794 6.1:-5527814 MS.gene074172:CDS
TTCATGATTGTGTTCCAACA+GGG 0.604224 6.1:-5527594 MS.gene074172:CDS
TGATTCTTATACACCGGACA+AGG 0.630256 6.1:-5527353 MS.gene074172:CDS
ACCCTACAATCAACAGCCAA+GGG 0.631197 6.1:-5526428 MS.gene074172:CDS
AGTGTTCACATTGCAAATGG+TGG 0.646316 6.1:-5527667 MS.gene074172:CDS
TAGCTGGAATTGGAGATCAG+AGG 0.658304 6.1:-5526328 MS.gene074172:CDS
GGGAGATGTATGCAATTGGT+GGG 0.665370 6.1:-5526458 MS.gene074172:CDS
AGTGATGGAACCAACAAGAG+AGG 0.669990 6.1:+5526212 None:intergenic
TTGTTGTACAAGATGTACAA+AGG 0.691137 6.1:-5528900 MS.gene074172:intron
GGCTTGTCCAAAGAATGCCA+AGG 0.703891 6.1:-5527291 MS.gene074172:intron
TTTGGAAAAGATGCAATGGG+TGG 0.760333 6.1:-5527880 MS.gene074172:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGCTAAAAAAAATATTAA+AGG + chr6.1:5527924-5527943 None:intergenic 10.0%
!! CTGAAAACAATAATTATTTA+TGG + chr6.1:5528627-5528646 None:intergenic 15.0%
!! GTTAATAATGTTATAATACA+TGG - chr6.1:5527527-5527546 MS.gene074172:CDS 15.0%
!! TAACCCAAATTAAAAAAATT+GGG - chr6.1:5528014-5528033 MS.gene074172:CDS 15.0%
!! TTCATAATATGTAATTAACT+TGG + chr6.1:5527028-5527047 None:intergenic 15.0%
!!! GTTCTTGTTTTTTTTTTTAA+AGG - chr6.1:5526814-5526833 MS.gene074172:intron 15.0%
!!! TAATATTTTTTTTAGCATAG+TGG - chr6.1:5527925-5527944 MS.gene074172:CDS 15.0%
!! AAATTCAAAAAGTAGGATAA+AGG - chr6.1:5528516-5528535 MS.gene074172:intron 20.0%
!! ATAAATGTTTGAAGAGAAAA+GGG + chr6.1:5526884-5526903 None:intergenic 20.0%
!! ATGATACTACTAAGAAATTA+GGG + chr6.1:5528412-5528431 None:intergenic 20.0%
!! CAATCACAAATGAAATTATT+TGG + chr6.1:5528147-5528166 None:intergenic 20.0%
!! CCTTAAAAAATTCAAAAAGT+AGG - chr6.1:5528509-5528528 MS.gene074172:intron 20.0%
!! CTAACCCAAATTAAAAAAAT+TGG - chr6.1:5528013-5528032 MS.gene074172:CDS 20.0%
!! CTATTTAGAAAGGAAAAAAA+AGG - chr6.1:5526766-5526785 MS.gene074172:intron 20.0%
!! GTTTAATTACTATACATTGA+CGG - chr6.1:5526942-5526961 MS.gene074172:intron 20.0%
!! TATAAATGTTTGAAGAGAAA+AGG + chr6.1:5526885-5526904 None:intergenic 20.0%
!! TCATATAAACCTATTTAGAA+AGG - chr6.1:5526756-5526775 MS.gene074172:intron 20.0%
!! TGTTTAATTCACAACATATT+TGG - chr6.1:5527651-5527670 MS.gene074172:CDS 20.0%
!!! ACTATTGCTTTTAATCATTT+TGG - chr6.1:5527833-5527852 MS.gene074172:CDS 20.0%
!!! ATGCCCAATTTTTTTAATTT+GGG + chr6.1:5528020-5528039 None:intergenic 20.0%
!!! ATTTTAATTTCTCTTCTTGT+AGG - chr6.1:5527114-5527133 MS.gene074172:intron 20.0%
!!! CCTACTTTTTGAATTTTTTA+AGG + chr6.1:5528512-5528531 None:intergenic 20.0%
!!! CTTTTTTTTCCTTTCTAAAT+AGG + chr6.1:5526768-5526787 None:intergenic 20.0%
!!! TTGAATTTTTTAAGGTAGTT+AGG + chr6.1:5528504-5528523 None:intergenic 20.0%
!!! TTTGTTTTTAAAAGAGAAAC+TGG - chr6.1:5528054-5528073 MS.gene074172:intron 20.0%
! AAAGAAAATCTCTCTATAGT+TGG + chr6.1:5528252-5528271 None:intergenic 25.0%
! AAGAAAATCTCTCTATAGTT+GGG + chr6.1:5528251-5528270 None:intergenic 25.0%
! AAGAGGATGAAACAAAATTT+GGG + chr6.1:5526214-5526233 None:intergenic 25.0%
! AATAGGTTTATATGACAATG+TGG + chr6.1:5526751-5526770 None:intergenic 25.0%
! AATTCATACAAGACAATTGA+TGG - chr6.1:5527452-5527471 MS.gene074172:CDS 25.0%
! AATTTCTTAGTAGTATCATG+AGG - chr6.1:5528413-5528432 MS.gene074172:intron 25.0%
! CATGATACTACTAAGAAATT+AGG + chr6.1:5528413-5528432 None:intergenic 25.0%
! GATCATATTCAAAAGAGATA+TGG - chr6.1:5527400-5527419 MS.gene074172:CDS 25.0%
!! AAAGAATGAGAAAGTATGAA+AGG + chr6.1:5526178-5526197 None:intergenic 25.0%
!! AAGAATGAGAAAGTATGAAA+GGG + chr6.1:5526177-5526196 None:intergenic 25.0%
!! CACATTTCTTTGAATTTGTT+AGG + chr6.1:5528467-5528486 None:intergenic 25.0%
!! CTTTTGAATATGATCCATAA+TGG + chr6.1:5527395-5527414 None:intergenic 25.0%
!! GTTAGCAAAAATGTGTTTTT+GGG - chr6.1:5527969-5527988 MS.gene074172:CDS 25.0%
!! TCAATTAAAACAAGAGCTTT+TGG - chr6.1:5527427-5527446 MS.gene074172:CDS 25.0%
!! TGTTAGCAAAAATGTGTTTT+TGG - chr6.1:5527968-5527987 MS.gene074172:CDS 25.0%
!!! CATGCCCAATTTTTTTAATT+TGG + chr6.1:5528021-5528040 None:intergenic 25.0%
!!! CTTTTAATCATTTTGGTGAA+GGG - chr6.1:5527840-5527859 MS.gene074172:CDS 25.0%
!!! GTCAAGTTCTTGTTTTTAAT+TGG - chr6.1:5526836-5526855 MS.gene074172:intron 25.0%
!!! TTACTTTGTTTTTGGTTCAA+TGG + chr6.1:5528441-5528460 None:intergenic 25.0%
!!! TTAGGTCTTTACTTTGTTTT+TGG + chr6.1:5528449-5528468 None:intergenic 25.0%
AACAGGGAACACAAATATAA+GGG - chr6.1:5527574-5527593 MS.gene074172:CDS 30.0%
ACATAGTGATTCTTATACAC+CGG - chr6.1:5527793-5527812 MS.gene074172:CDS 30.0%
ACCAGATAATAGATTTAGCA+AGG - chr6.1:5528761-5528780 MS.gene074172:intron 30.0%
AGAAAGGAAAAAAAAGGCTT+TGG - chr6.1:5526772-5526791 MS.gene074172:intron 30.0%
CAATTCAAGATGAACCATTA+TGG - chr6.1:5527378-5527397 MS.gene074172:CDS 30.0%
CAGAAAGTGAATATAATAGC+TGG - chr6.1:5528808-5528827 MS.gene074172:intron 30.0%
GAACAATGATTACACACATT+GGG - chr6.1:5528674-5528693 MS.gene074172:intron 30.0%
GAAGAGGATGAAACAAAATT+TGG + chr6.1:5526215-5526234 None:intergenic 30.0%
GTGAATATAATAGCTGGAAT+TGG - chr6.1:5528814-5528833 MS.gene074172:intron 30.0%
TCCTTGCTAAATCTATTATC+TGG + chr6.1:5528765-5528784 None:intergenic 30.0%
TGAACAATGATTACACACAT+TGG - chr6.1:5528673-5528692 MS.gene074172:intron 30.0%
TGTCATATATATGCATACCT+TGG + chr6.1:5527881-5527900 None:intergenic 30.0%
TGTGTAAGATCAACTTCTTT+AGG - chr6.1:5528171-5528190 MS.gene074172:intron 30.0%
TTGTTGTACAAGATGTACAA+AGG - chr6.1:5526252-5526271 MS.gene074172:CDS 30.0%
! ATTGTTTTCAGATGTAGACA+TGG - chr6.1:5528636-5528655 MS.gene074172:intron 30.0%
! CATGACAAAGTTATGCTTTT+GGG - chr6.1:5527770-5527789 MS.gene074172:CDS 30.0%
! GCTATTATGAGAGATTTTGA+AGG + chr6.1:5528369-5528388 None:intergenic 30.0%
! GTTTAATAGAAGTGCATTGT+TGG - chr6.1:5526687-5526706 MS.gene074172:intron 30.0%
! TCATGACAAAGTTATGCTTT+TGG - chr6.1:5527769-5527788 MS.gene074172:CDS 30.0%
!! GCTTTTAATCATTTTGGTGA+AGG - chr6.1:5527839-5527858 MS.gene074172:CDS 30.0%
!! GGTCTTATTGATGTTATACA+TGG - chr6.1:5527707-5527726 MS.gene074172:CDS 30.0%
AATTCCATCACCATCAGAAA+CGG + chr6.1:5527627-5527646 None:intergenic 35.0%
ACAATGTGGTGAATTATCGT+TGG + chr6.1:5526737-5526756 None:intergenic 35.0%
ACATCTTGTACAACAAGTTC+AGG + chr6.1:5526248-5526267 None:intergenic 35.0%
CAACAGGGAACACAAATATA+AGG - chr6.1:5527573-5527592 MS.gene074172:CDS 35.0%
CATCTCCAACAATCATCAAT+TGG + chr6.1:5527200-5527219 None:intergenic 35.0%
CATCTTGTACAACAAGTTCA+GGG + chr6.1:5526247-5526266 None:intergenic 35.0%
CTTATATTTGTGTTCCCTGT+TGG + chr6.1:5527575-5527594 None:intergenic 35.0%
GGATTTGGAAAAGATGCAAT+GGG - chr6.1:5527269-5527288 MS.gene074172:intron 35.0%
GGGAGTAGAAATATTTGCTT+AGG - chr6.1:5527989-5528008 MS.gene074172:CDS 35.0%
TACAAGACAATTGATGGAAG+AGG - chr6.1:5527458-5527477 MS.gene074172:CDS 35.0%
TCTTTGTCTAATTGTCGTGA+TGG - chr6.1:5527686-5527705 MS.gene074172:CDS 35.0%
TGGATTTGGAAAAGATGCAA+TGG - chr6.1:5527268-5527287 MS.gene074172:intron 35.0%
TTCATGATTGTGTTCCAACA+GGG - chr6.1:5527558-5527577 MS.gene074172:CDS 35.0%
! GCAATCCAATTGATGATTGT+TGG - chr6.1:5527192-5527211 MS.gene074172:intron 35.0%
! GGAGAGAGAATTTTCTATCA+CGG - chr6.1:5528192-5528211 MS.gene074172:intron 35.0%
! TGTCACTAGATGCATGTTTT+TGG + chr6.1:5526411-5526430 None:intergenic 35.0%
!! ATCTACATTGTCACTGATTC+AGG - chr6.1:5527308-5527327 MS.gene074172:CDS 35.0%
!! TAGACATGGCTATTTTCATG+TGG - chr6.1:5528650-5528669 MS.gene074172:intron 35.0%
AAGCCAGGAACATTAAGGTA+TGG - chr6.1:5527353-5527372 MS.gene074172:CDS 40.0%
ACCTTAATGTTCCTGGCTTA+GGG + chr6.1:5527352-5527371 None:intergenic 40.0%
AGCTTGGGCTATTTATCATG+TGG - chr6.1:5527164-5527183 MS.gene074172:intron 40.0%
AGTGTTCACATTGCAAATGG+TGG - chr6.1:5527485-5527504 MS.gene074172:CDS 40.0%
ATGCATACCTTGGCATTCTT+TGG + chr6.1:5527871-5527890 None:intergenic 40.0%
ATGTAGATAACCCCACAAGT+GGG - chr6.1:5528286-5528305 MS.gene074172:intron 40.0%
ATTTATGGGTTCCCACTTGT+GGG + chr6.1:5528300-5528319 None:intergenic 40.0%
CATTGGGAGATGTATGCAAT+TGG - chr6.1:5528690-5528709 MS.gene074172:intron 40.0%
GATGCAATGGGTGGTAAAAA+TGG - chr6.1:5527281-5527300 MS.gene074172:intron 40.0%
GCTAGTGTTCACATTGCAAA+TGG - chr6.1:5527482-5527501 MS.gene074172:CDS 40.0%
GCTGATTGTGCAATTGGATT+TGG - chr6.1:5527254-5527273 MS.gene074172:intron 40.0%
GGCTATTTATCATGTGGAAC+TGG - chr6.1:5527170-5527189 MS.gene074172:intron 40.0%
GGGGTTATCTACATAGAGAT+GGG + chr6.1:5528280-5528299 None:intergenic 40.0%
GGTGAATTATCGTTGGATGT+TGG + chr6.1:5526730-5526749 None:intergenic 40.0%
GTCCAAAATCCACTATGTTC+TGG + chr6.1:5527605-5527624 None:intergenic 40.0%
GTTCATGATTGTGTTCCAAC+AGG - chr6.1:5527557-5527576 MS.gene074172:CDS 40.0%
TACCTTAATGTTCCTGGCTT+AGG + chr6.1:5527353-5527372 None:intergenic 40.0%
TATGTAGATAACCCCACAAG+TGG - chr6.1:5528285-5528304 MS.gene074172:intron 40.0%
TATTTATGGGTTCCCACTTG+TGG + chr6.1:5528301-5528320 None:intergenic 40.0%
TCTCTCTATAGTTGGGAGTA+TGG + chr6.1:5528244-5528263 None:intergenic 40.0%
TGATTCTTATACACCGGACA+AGG - chr6.1:5527799-5527818 MS.gene074172:CDS 40.0%
TGGGGTTATCTACATAGAGA+TGG + chr6.1:5528281-5528300 None:intergenic 40.0%
TTGGAGATGTGACCCAAATT+GGG - chr6.1:5527211-5527230 MS.gene074172:intron 40.0%
TTTGGAAAAGATGCAATGGG+TGG - chr6.1:5527272-5527291 MS.gene074172:intron 40.0%
! AGACTAGCTGATTGTGCAAT+TGG - chr6.1:5527248-5527267 MS.gene074172:intron 40.0%
! CTCTCTATAGTTGGGAGTAT+GGG + chr6.1:5528243-5528262 None:intergenic 40.0%
! GGAGCATTCTTTAGACAAAC+TGG - chr6.1:5528864-5528883 MS.gene074172:intron 40.0%
! TCTCTATAGTTGGGAGTATG+GGG + chr6.1:5528242-5528261 None:intergenic 40.0%
!! CACAATCAGCTAGTCTTTGT+CGG + chr6.1:5527244-5527263 None:intergenic 40.0%
!! GAGGGTGATTTGTTCTTGAA+TGG - chr6.1:5528843-5528862 MS.gene074172:intron 40.0%
!!! CACCAGAACATAGTGGATTT+TGG - chr6.1:5527600-5527619 MS.gene074172:CDS 40.0%
AACCCTACAATCAACAGCCA+AGG - chr6.1:5528723-5528742 MS.gene074172:intron 45.0%
ACCCTAAGCCAGGAACATTA+AGG - chr6.1:5527348-5527367 MS.gene074172:CDS 45.0%
ACCCTACAATCAACAGCCAA+GGG - chr6.1:5528724-5528743 MS.gene074172:intron 45.0%
AGCTGGAATTGGAGATCAGA+GGG - chr6.1:5528825-5528844 MS.gene074172:intron 45.0%
AGGGATTCACCAGAACATAG+TGG - chr6.1:5527593-5527612 MS.gene074172:CDS 45.0%
CACACCAGAAGTAGTAGTGA+TGG + chr6.1:5528958-5528977 None:intergenic 45.0%
CGTTGGATGTTGGTGTAAGA+TGG + chr6.1:5526720-5526739 None:intergenic 45.0%
GCACCATACCTTAATGTTCC+TGG + chr6.1:5527359-5527378 None:intergenic 45.0%
GCATCAATGTCTCTAGGTCT+AGG - chr6.1:5527138-5527157 MS.gene074172:intron 45.0%
GGGAGATGTATGCAATTGGT+GGG - chr6.1:5528694-5528713 MS.gene074172:intron 45.0%
GGTGTGCTTACTTGCAAGAA+GGG - chr6.1:5528972-5528991 MS.gene074172:CDS 45.0%
GGTTCCATCACTACTACTTC+TGG - chr6.1:5528951-5528970 MS.gene074172:CDS 45.0%
GTCGGTTGTTCTCCCAATTT+GGG + chr6.1:5527226-5527245 None:intergenic 45.0%
GTGCAAGAAATCTGTTCCCT+TGG + chr6.1:5528743-5528762 None:intergenic 45.0%
GTTGGAGATGTGACCCAAAT+TGG - chr6.1:5527210-5527229 MS.gene074172:intron 45.0%
TAGCTGGAATTGGAGATCAG+AGG - chr6.1:5528824-5528843 MS.gene074172:intron 45.0%
TCTAGGTCTAGGAGAAGCTT+GGG - chr6.1:5527149-5527168 MS.gene074172:intron 45.0%
TGGGAGATGTATGCAATTGG+TGG - chr6.1:5528693-5528712 MS.gene074172:intron 45.0%
TGGTGTGCTTACTTGCAAGA+AGG - chr6.1:5528971-5528990 MS.gene074172:CDS 45.0%
TTCAGGGTCTTGAACTGAAG+AGG + chr6.1:5526231-5526250 None:intergenic 45.0%
TTTATGGGTTCCCACTTGTG+GGG + chr6.1:5528299-5528318 None:intergenic 45.0%
! AGTGATGGAACCAACAAGAG+AGG + chr6.1:5528943-5528962 None:intergenic 45.0%
! GGATTTTGGACCGTTTCTGA+TGG - chr6.1:5527614-5527633 MS.gene074172:CDS 45.0%
! TCCCTTGGCTGTTGATTGTA+GGG + chr6.1:5528728-5528747 None:intergenic 45.0%
! TGTCGGTTGTTCTCCCAATT+TGG + chr6.1:5527227-5527246 None:intergenic 45.0%
! TTCCCTTGGCTGTTGATTGT+AGG + chr6.1:5528729-5528748 None:intergenic 45.0%
!! GTAGGAGCATCAATGTCTCT+AGG - chr6.1:5527132-5527151 MS.gene074172:intron 45.0%
!!! ACATTTTTTTTTATATTTAT+GGG + chr6.1:5528314-5528333 None:intergenic 5.0%
!!! TACATTTTTTTTTATATTTA+TGG + chr6.1:5528315-5528334 None:intergenic 5.0%
ATGGAACCAACAAGAGAGGC+TGG + chr6.1:5528939-5528958 None:intergenic 50.0%
CTCTAGGTCTAGGAGAAGCT+TGG - chr6.1:5527148-5527167 MS.gene074172:intron 50.0%
GATGCAGTAAACCCTAAGCC+AGG - chr6.1:5527338-5527357 MS.gene074172:CDS 50.0%
GGCTTGTCCAAAGAATGCCA+AGG - chr6.1:5527861-5527880 MS.gene074172:CDS 50.0%
! TGGACCGTTTCTGATGGTGA+TGG - chr6.1:5527620-5527639 MS.gene074172:CDS 50.0%
GCAAGACCAGCCTCTCTTGT+TGG - chr6.1:5528930-5528949 MS.gene074172:CDS 55.0%
GTGACCTGCATGTCCTTGTC+CGG + chr6.1:5527815-5527834 None:intergenic 55.0%
TACACCGGACAAGGACATGC+AGG - chr6.1:5527808-5527827 MS.gene074172:CDS 55.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 5526167 5529007 5526167 ID=MS.gene074172
chr6.1 mRNA 5526167 5529007 5526167 ID=MS.gene074172.t1;Parent=MS.gene074172
chr6.1 exon 5528901 5529007 5528901 ID=MS.gene074172.t1.exon1;Parent=MS.gene074172.t1
chr6.1 CDS 5528901 5529007 5528901 ID=cds.MS.gene074172.t1;Parent=MS.gene074172.t1
chr6.1 exon 5527292 5528038 5527292 ID=MS.gene074172.t1.exon2;Parent=MS.gene074172.t1
chr6.1 CDS 5527292 5528038 5527292 ID=cds.MS.gene074172.t1;Parent=MS.gene074172.t1
chr6.1 exon 5526167 5526527 5526167 ID=MS.gene074172.t1.exon3;Parent=MS.gene074172.t1
chr6.1 CDS 5526167 5526527 5526167 ID=cds.MS.gene074172.t1;Parent=MS.gene074172.t1
Gene Sequence

>MS.gene074172

ATGACTTCCCTTTCATACTTTCTCATTCTTTTTGCTCTTCTAATCCCAAATTTTGTTTCATCCTCTTCAGTTCAAGACCCTGAACTTGTTGTACAAGATGTACAAAGGTAATAACATTTCTTTATTTCTTAATTATGTTGTGTTATGTGTACTTACTCTAGCTACATTATTCATTTTATGAGTATTTCGATCAACAATTTCATCAAGTTTATTGCATAAAAGTTTGATTCATATGTAATGTCCAAAAACATGCATCTAGTGACATATGATAAAATAAATATTTTGTGCATCTAACATAACAAAGTGATATCATAGAGTAGTTTGTTTTGAATGCATGTGTACTTTTTTTTTACACTATAAATTCATAAAAATTAATCTCAAAGTTTTTTTTTTTTTTTTGAGAGAATTAATCTCAAAGCTCATATGTGTTATTTCTAACATTTGATTATAGAATCAAAATATCTCAAAATCAGTTAATGAAAATGTTGTGAGTTATTTTTATAAACTCTTCTAAACACGTGTTTAATAGAAGTGCATTGTTGGTGTAAGACCATCTTACACCAACATCCAACGATAATTCACCACATTGTCATATAAACCTATTTAGAAAGGAAAAAAAAGGCTTTGGCTTGTTAAAAAAAAGTCAAGTTCTTGTTTTTTTTTTTAAAGGTCAAGTTCTTGTTTTTAATTGGTTGTTAATGTAAAACTAGTTATCCCTTTTCTCTTCAAACATTTATATGTAAATACTTATGTCATACTATAATAACTTCAAAGAGTTTAATTACTATACATTGACGGTGTAAAGTTATTTTACATATCAATGTGTTGTTACATTAATTAATGTCATCAACTTCAACGCCAAGTTAATTACATATTATGAAGTATAAATATTATGAAAACAAAATAAATTTTAATATAATAATTGTTAGATTTGAATTAATTAATTAATTTTAATTTCTCTTCTTGTAGGAGCATCAATGTCTCTAGGTCTAGGAGAAGCTTGGGCTATTTATCATGTGGAACTGGCAATCCAATTGATGATTGTTGGAGATGTGACCCAAATTGGGAGAACAACCGACAAAGACTAGCTGATTGTGCAATTGGATTTGGAAAAGATGCAATGGGTGGTAAAAATGGTAAAATCTACATTGTCACTGATTCAGGTGATGATGATGCAGTAAACCCTAAGCCAGGAACATTAAGGTATGGTGCAATTCAAGATGAACCATTATGGATCATATTCAAAAGAGATATGGTAATTCAATTAAAACAAGAGCTTTTGGTAAATTCATACAAGACAATTGATGGAAGAGGTGCTAGTGTTCACATTGCAAATGGTGGATGTATTACAATACATTATGTTAATAATGTTATAATACATGGTATTCATGTTCATGATTGTGTTCCAACAGGGAACACAAATATAAGGGATTCACCAGAACATAGTGGATTTTGGACCGTTTCTGATGGTGATGGAATTTCAGTGTTTAATTCACAACATATTTGGATTGATCATTGTTCTTTGTCTAATTGTCGTGATGGTCTTATTGATGTTATACATGGATCTAATGCTATTACTATTTCAAATAACTATATGACTCATCATGACAAAGTTATGCTTTTGGGACATAGTGATTCTTATACACCGGACAAGGACATGCAGGTCACTATTGCTTTTAATCATTTTGGTGAAGGGCTTGTCCAAAGAATGCCAAGGTATGCATATATATGACACTAAAATTTCACTTTTTTTTCCTTTAATATTTTTTTTAGCATAGTGGTAAAAAAAAAAGTCATGCATTGTTAGCAAAAATGTGTTTTTGGGAGTAGAAATATTTGCTTAGGACTAACCCAAATTAAAAAAATTGGGCATGCTCACACATATATTTTGTTTTTAAAAGAGAAACTGGTTTAATGATGTTGAATAACGTGACTAATTTATTTATCTATTAATTTATTTTATTATTATGTGTGTGTCCAAATAATTTCATTTGTGATTGCGTTTGTGTAAGATCAACTTCTTTAGGAGAGAGAATTTTCTATCACGGCATCTCAAAATGTCGATCGATATGCCCCATACTCCCAACTATAGAGAGATTTTCTTTTATGTCCCATCTCTATGTAGATAACCCCACAAGTGGGAACCCATAAATATAAAAAAAAATGTAAATTTTATTATTGAAAATTGTCATTTTCTAACCTTCAAAATCTCTCATAATAGCAAATCTCATATTAATGTCAACCCTAATTTCTTAGTAGTATCATGAGGCTCCATTGAACCAAAAACAAAGTAAAGACCTAACAAATTCAAAGAAATGTGCATATATACTCATACCTAACTACCTTAAAAAATTCAAAAAGTAGGATAAAGGTTAGTTGTGAATTTTTTAGAGTTATCTATTGCTTATTATTTGTTCAAACACTCACATGAATATATATAAGCTTGAAAATATATAACCATAAATAATTATTGTTTTCAGATGTAGACATGGCTATTTTCATGTGGTGAACAATGATTACACACATTGGGAGATGTATGCAATTGGTGGGAGTGCTAACCCTACAATCAACAGCCAAGGGAACAGATTTCTTGCACCAGATAATAGATTTAGCAAGGAAGTAACAAAGCATGAAGATGCATCAGAAAGTGAATATAATAGCTGGAATTGGAGATCAGAGGGTGATTTGTTCTTGAATGGAGCATTCTTTAGACAAACTGGTGCTGAATCTTCTTCAATCTATGCTAGAGCTTCAAGTCTTAGTGCAAGACCAGCCTCTCTTGTTGGTTCCATCACTACTACTTCTGGTGTGCTTACTTGCAAGAAGGGCAACCGTTGCTAA
Protein sequence

>MS.gene074172.t1

MTSLSYFLILFALLIPNFVSSSSVQDPELVVQDVQRSINVSRSRRSLGYLSCGTGNPIDDCWRCDPNWENNRQRLADCAIGFGKDAMGGKNGKIYIVTDSGDDDAVNPKPGTLRYGAIQDEPLWIIFKRDMVIQLKQELLVNSYKTIDGRGASVHIANGGCITIHYVNNVIIHGIHVHDCVPTGNTNIRDSPEHSGFWTVSDGDGISVFNSQHIWIDHCSLSNCRDGLIDVIHGSNAITISNNYMTHHDKVMLLGHSDSYTPDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFLAPDNRFSKEVTKHEDASESEYNSWNWRSEGDLFLNGAFFRQTGAESSSIYARASSLSARPASLVGSITTTSGVLTCKKGNRC