AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene074408


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene074408.t1 MTR_3g078800 91.270 252 17 1 1 247 1 252 5.88e-151 421
MS.gene074408.t1 MTR_5g087760 63.281 256 85 3 1 248 1 255 8.65e-119 339
MS.gene074408.t1 MTR_2g012550 49.615 260 109 5 1 241 1 257 1.03e-79 241
MS.gene074408.t1 MTR_3g078810 68.493 146 44 2 104 247 1 146 1.21e-68 208
MS.gene074408.t1 MTR_3g078820 56.784 199 84 2 43 241 4 200 5.01e-65 201
MS.gene074408.t1 MTR_1g077560 32.669 251 158 5 3 249 7 250 2.15e-34 124
MS.gene074408.t1 MTR_5g076170 31.818 264 162 6 1 247 1 263 1.41e-33 122
MS.gene074408.t1 MTR_3g063310 36.782 174 99 3 81 249 85 252 4.83e-30 112
MS.gene074408.t1 MTR_3g074150 27.876 226 142 7 13 230 16 228 2.11e-26 103
MS.gene074408.t1 MTR_8g090320 28.755 233 142 8 24 248 43 259 4.59e-23 94.7
MS.gene074408.t1 MTR_3g104460 28.358 201 131 6 57 247 56 253 1.92e-19 85.1
MS.gene074408.t1 MTR_4g119428 30.918 207 119 6 38 227 17 216 5.03e-19 83.6
MS.gene074408.t1 MTR_2g100060 27.473 182 120 4 77 248 77 256 1.18e-17 80.1
MS.gene074408.t1 MTR_2g090225 26.699 206 147 3 44 247 17 220 3.35e-16 75.5
MS.gene074408.t1 MTR_7g106000 25.463 216 148 7 36 245 18 226 4.12e-16 75.1
MS.gene074408.t1 MTR_2g011350 27.027 148 106 2 76 223 35 180 1.13e-14 70.9
MS.gene074408.t1 MTR_7g105970 27.717 184 120 7 51 227 29 206 9.19e-14 68.9
MS.gene074408.t1 MTR_1g057270 25.568 176 120 4 82 248 74 247 1.13e-13 68.9
MS.gene074408.t1 MTR_8g016370 25.397 252 143 9 5 248 7 221 1.39e-13 68.2
MS.gene074408.t1 MTR_7g106030 28.108 185 118 8 52 227 29 207 9.94e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene074408.t1 AT2G27080 52.107 261 111 2 1 248 1 260 5.33e-97 285
MS.gene074408.t1 AT2G27080 52.107 261 111 2 1 248 1 260 5.33e-97 285
MS.gene074408.t1 AT5G21130 45.852 229 123 1 19 247 53 280 6.34e-71 219
MS.gene074408.t1 AT5G36970 33.205 259 150 9 1 247 1 248 6.23e-33 120
MS.gene074408.t1 AT1G65690 32.245 245 159 4 7 249 13 252 4.74e-29 110
MS.gene074408.t1 AT1G54540 32.540 252 152 7 1 247 1 239 2.95e-28 108
MS.gene074408.t1 AT2G35980 32.461 191 121 6 36 224 16 200 9.77e-24 95.9
MS.gene074408.t1 AT2G22180 27.542 236 141 8 19 245 75 289 1.21e-20 89.0
MS.gene074408.t1 AT3G11650 27.228 202 129 5 36 224 17 213 4.47e-17 78.2
MS.gene074408.t1 AT5G22870 25.405 185 126 4 62 241 26 203 1.44e-16 76.3
MS.gene074408.t1 AT5G06320 30.000 170 113 5 61 227 44 210 3.76e-16 75.5
MS.gene074408.t1 AT5G11890 28.866 194 129 4 39 224 60 252 1.24e-11 63.5
MS.gene074408.t1 AT1G17620 29.258 229 152 6 3 224 4 229 1.28e-11 63.5
MS.gene074408.t1 AT4G09590 24.468 188 138 3 60 247 20 203 2.53e-11 61.6
MS.gene074408.t1 AT3G52470 24.444 180 133 2 68 247 24 200 6.20e-11 60.8
MS.gene074408.t1 AT5G53730 26.351 148 98 5 82 223 44 186 7.60e-11 60.5

Find 67 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGCTGTTGCTGTCTTTGC+TGG 0.303551 3.4:+75327052 MS.gene074408:CDS
ATGCGGTAGGTTTGATCCTT+GGG 0.329031 3.4:-75326967 None:intergenic
GTAGTTATGAATTTGATCTT+TGG 0.333495 3.4:+75327604 MS.gene074408:CDS
ATTGTGTATTTCGGTGCTTC+CGG 0.342584 3.4:-75327147 None:intergenic
TTGGAAGGTGGAGAGGCGTT+AGG 0.343094 3.4:-75326901 None:intergenic
GACGTGGAAGGTTAGAGTTA+AGG 0.387882 3.4:+75327521 MS.gene074408:CDS
AGGTTTGATCCTTGGGGAGC+TGG 0.388163 3.4:-75326960 None:intergenic
GTGCTCATGGCCATCGCTGC+CGG 0.415051 3.4:+75327102 MS.gene074408:CDS
CGGCGGCGATGGAGCGGTGT+TGG 0.430905 3.4:-75326920 None:intergenic
GATGCGGTAGGTTTGATCCT+TGG 0.434091 3.4:-75326968 None:intergenic
CGGCATTGAACTTGCGAGAT+CGG 0.437793 3.4:+75327401 MS.gene074408:CDS
GCGTAACGTTGTGCATTCTC+TGG 0.446865 3.4:-75326997 None:intergenic
ACCGCTCCATCGCCGCCGTC+CGG 0.448630 3.4:+75326925 MS.gene074408:CDS
TGTTAGGTTCATTCCCTTGA+CGG 0.454596 3.4:-75327181 None:intergenic
ACACGATCGATTGTGTATTT+CGG 0.457527 3.4:-75327156 None:intergenic
TGCTGTCTTTGCTGGTTCAT+CGG 0.470949 3.4:+75327060 MS.gene074408:CDS
AAATAGTAGATAAAGATGTT+AGG 0.480050 3.4:-75327197 None:intergenic
CGTTGTGCATTCTCTGGTGG+TGG 0.481192 3.4:-75326991 None:intergenic
TGGAAGGTGGAGAGGCGTTA+GGG 0.484091 3.4:-75326900 None:intergenic
GTTTCTGTTAAAGCTGATAA+CGG 0.484250 3.4:+75327234 MS.gene074408:CDS
GGCGATGGAGCGGTGTTGGA+AGG 0.486020 3.4:-75326916 None:intergenic
ATTTACTCTGATCGTGCTCA+TGG 0.493417 3.4:+75327089 MS.gene074408:CDS
TCTCTGTAACGGCGTCCTGC+CGG 0.493799 3.4:+75327323 MS.gene074408:CDS
CTCTGGTGGTGGGATGCGGT+AGG 0.506358 3.4:-75326980 None:intergenic
AGCGTTGGTGAATGCGGTTA+CGG 0.509259 3.4:+75327431 MS.gene074408:CDS
GAGCTGGATGACATATGTTC+CGG 0.509609 3.4:-75326944 None:intergenic
GCTCTCAGCTTCAACGTTAA+CGG 0.515780 3.4:-75327465 None:intergenic
CCTCCAACTTTAATCTTCAC+CGG 0.526185 3.4:-75327489 None:intergenic
CATTCTCTGGTGGTGGGATG+CGG 0.527360 3.4:-75326984 None:intergenic
AATCGATCGTGTCTCCGTCA+AGG 0.528158 3.4:+75327167 MS.gene074408:CDS
ATCGATCGTGTCTCCGTCAA+GGG 0.528651 3.4:+75327168 MS.gene074408:CDS
ACGGCTGATAAAACGCCGGC+AGG 0.534301 3.4:-75327338 None:intergenic
TATGTTCCGGACGGCGGCGA+TGG 0.535007 3.4:-75326931 None:intergenic
TCTAACGGTGGATCAGTTAA+CGG 0.540813 3.4:+75327554 MS.gene074408:CDS
TCCGGACGGCGGCGATGGAG+CGG 0.543871 3.4:-75326926 None:intergenic
GCACCGGTGAAGATTAAAGT+TGG 0.544024 3.4:+75327486 MS.gene074408:CDS
GGGTGAACACGATCAGCCAT+TGG 0.548503 3.4:-75326880 None:intergenic
GACAGCAACAGCATCCGCAC+CGG 0.556268 3.4:-75327044 None:intergenic
CAGACGGTGTTGAAAGGTAA+CGG 0.567575 3.4:+75327381 MS.gene074408:CDS
GTGTTTCAGACGGTGTTGAA+AGG 0.576347 3.4:+75327375 MS.gene074408:CDS
GGTGGATCAGTTAACGGCGC+AGG 0.585762 3.4:+75327560 MS.gene074408:CDS
TCAGAGAGCGTTGGTGAATG+CGG 0.587887 3.4:+75327425 MS.gene074408:CDS
AACGTTGAAGCTGAGAGCAC+CGG 0.589685 3.4:+75327470 MS.gene074408:CDS
GATGGAGCGGTGTTGGAAGG+TGG 0.592952 3.4:-75326913 None:intergenic
CCGGTGAAGATTAAAGTTGG+AGG 0.596076 3.4:+75327489 MS.gene074408:CDS
AGCGGTGTTGGAAGGTGGAG+AGG 0.599059 3.4:-75326908 None:intergenic
CGCCGCAAAAGTCGCCGGTG+CGG 0.603490 3.4:+75327030 MS.gene074408:CDS
ACACTCGCCGCAAAAGTCGC+CGG 0.610216 3.4:+75327025 MS.gene074408:CDS
TAACGTGACGGTGTTTCAGA+CGG 0.610689 3.4:+75327365 MS.gene074408:CDS
TAAGGTTGACTGTGATCTAA+CGG 0.613562 3.4:+75327539 MS.gene074408:CDS
TTTAACAGAAACATCAAACT+CGG 0.615677 3.4:-75327223 None:intergenic
TGCGGTAGGTTTGATCCTTG+GGG 0.618357 3.4:-75326966 None:intergenic
GCAGGCGAAGATTGTTGACA+AGG 0.624423 3.4:+75327578 MS.gene074408:CDS
AACACCGTCACGTTATTCGA+CGG 0.636233 3.4:-75327357 None:intergenic
GTTGTGCATTCTCTGGTGGT+GGG 0.643760 3.4:-75326990 None:intergenic
TAACGTTGTGCATTCTCTGG+TGG 0.648494 3.4:-75326994 None:intergenic
TTGGAGGATCTGTGAAGACG+TGG 0.649777 3.4:+75327505 MS.gene074408:CDS
TTAACAGAAACATCAAACTC+GGG 0.650957 3.4:-75327222 None:intergenic
GAGATCGGATCAGAGAGCGT+TGG 0.672799 3.4:+75327416 MS.gene074408:CDS
AGAGGCGTTAGTCTCTGTAA+CGG 0.674629 3.4:+75327312 MS.gene074408:CDS
AGGATCTGTGAAGACGTGGA+AGG 0.675912 3.4:+75327509 MS.gene074408:CDS
ATATGTCATCCAGCTCCCCA+AGG 0.678653 3.4:+75326951 MS.gene074408:CDS
ATGACATATGTTCCGGACGG+CGG 0.681432 3.4:-75326937 None:intergenic
TGGATGACATATGTTCCGGA+CGG 0.702336 3.4:-75326940 None:intergenic
TTCGACGGCTGATAAAACGC+CGG 0.712718 3.4:-75327342 None:intergenic
GGTTGACTGTGATCTAACGG+TGG 0.726582 3.4:+75327542 MS.gene074408:CDS
TCAGCCGTCGAATAACGTGA+CGG 0.740743 3.4:+75327353 MS.gene074408:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAGTAGATAAAGATGTT+AGG - chr3.4:75327200-75327219 None:intergenic 20.0%
! GTAGTTATGAATTTGATCTT+TGG + chr3.4:75327604-75327623 MS.gene074408:CDS 25.0%
! TTTAACAGAAACATCAAACT+CGG - chr3.4:75327226-75327245 None:intergenic 25.0%
AACGATCAAGTAAAAAACAC+CGG - chr3.4:75327124-75327143 None:intergenic 30.0%
GTTTCTGTTAAAGCTGATAA+CGG + chr3.4:75327234-75327253 MS.gene074408:CDS 30.0%
TTAACAGAAACATCAAACTC+GGG - chr3.4:75327225-75327244 None:intergenic 30.0%
! GATAACGGTAACAAAAAGAT+CGG + chr3.4:75327249-75327268 MS.gene074408:CDS 30.0%
ACACGATCGATTGTGTATTT+CGG - chr3.4:75327159-75327178 None:intergenic 35.0%
TAAGGTTGACTGTGATCTAA+CGG + chr3.4:75327539-75327558 MS.gene074408:CDS 35.0%
! TTTTTACTTGATCGTTCGTC+CGG + chr3.4:75327128-75327147 MS.gene074408:CDS 35.0%
!! AGCGTTGAAATGTTTTACAG+AGG + chr3.4:75327294-75327313 MS.gene074408:CDS 35.0%
ATTTACTCTGATCGTGCTCA+TGG + chr3.4:75327089-75327108 MS.gene074408:CDS 40.0%
CCTCCAACTTTAATCTTCAC+CGG - chr3.4:75327492-75327511 None:intergenic 40.0%
TCTAACGGTGGATCAGTTAA+CGG + chr3.4:75327554-75327573 MS.gene074408:CDS 40.0%
TGTTAGGTTCATTCCCTTGA+CGG - chr3.4:75327184-75327203 None:intergenic 40.0%
!! ATTGTGTATTTCGGTGCTTC+CGG - chr3.4:75327150-75327169 None:intergenic 40.0%
AACACCGTCACGTTATTCGA+CGG - chr3.4:75327360-75327379 None:intergenic 45.0%
ATGCGGTAGGTTTGATCCTT+GGG - chr3.4:75326970-75326989 None:intergenic 45.0%
CCGGTGAAGATTAAAGTTGG+AGG + chr3.4:75327489-75327508 MS.gene074408:CDS 45.0%
GACGTGGAAGGTTAGAGTTA+AGG + chr3.4:75327521-75327540 MS.gene074408:CDS 45.0%
GAGCTGGATGACATATGTTC+CGG - chr3.4:75326947-75326966 None:intergenic 45.0%
GCTCTCAGCTTCAACGTTAA+CGG - chr3.4:75327468-75327487 None:intergenic 45.0%
TAACGTGACGGTGTTTCAGA+CGG + chr3.4:75327365-75327384 MS.gene074408:CDS 45.0%
TAACGTTGTGCATTCTCTGG+TGG - chr3.4:75326997-75327016 None:intergenic 45.0%
TGGATGACATATGTTCCGGA+CGG - chr3.4:75326943-75326962 None:intergenic 45.0%
! CAGACGGTGTTGAAAGGTAA+CGG + chr3.4:75327381-75327400 MS.gene074408:CDS 45.0%
! GCACCGGTGAAGATTAAAGT+TGG + chr3.4:75327486-75327505 MS.gene074408:CDS 45.0%
! GTGTTTCAGACGGTGTTGAA+AGG + chr3.4:75327375-75327394 MS.gene074408:CDS 45.0%
!! AGAGGCGTTAGTCTCTGTAA+CGG + chr3.4:75327312-75327331 MS.gene074408:CDS 45.0%
!! TGCTGTCTTTGCTGGTTCAT+CGG + chr3.4:75327060-75327079 MS.gene074408:CDS 45.0%
AATCGATCGTGTCTCCGTCA+AGG + chr3.4:75327167-75327186 MS.gene074408:CDS 50.0%
AGGATCTGTGAAGACGTGGA+AGG + chr3.4:75327509-75327528 MS.gene074408:CDS 50.0%
ATATGTCATCCAGCTCCCCA+AGG + chr3.4:75326951-75326970 MS.gene074408:CDS 50.0%
ATCGATCGTGTCTCCGTCAA+GGG + chr3.4:75327168-75327187 MS.gene074408:CDS 50.0%
ATGACATATGTTCCGGACGG+CGG - chr3.4:75326940-75326959 None:intergenic 50.0%
GATGCGGTAGGTTTGATCCT+TGG - chr3.4:75326971-75326990 None:intergenic 50.0%
GATGCTGTTGCTGTCTTTGC+TGG + chr3.4:75327052-75327071 MS.gene074408:CDS 50.0%
GCAGGCGAAGATTGTTGACA+AGG + chr3.4:75327578-75327597 MS.gene074408:CDS 50.0%
GCGTAACGTTGTGCATTCTC+TGG - chr3.4:75327000-75327019 None:intergenic 50.0%
GGTTGACTGTGATCTAACGG+TGG + chr3.4:75327542-75327561 MS.gene074408:CDS 50.0%
GTAAAAAACACCGGCAGCGA+TGG - chr3.4:75327115-75327134 None:intergenic 50.0%
GTTGTGCATTCTCTGGTGGT+GGG - chr3.4:75326993-75327012 None:intergenic 50.0%
TCAGCCGTCGAATAACGTGA+CGG + chr3.4:75327353-75327372 MS.gene074408:CDS 50.0%
TGCGGTAGGTTTGATCCTTG+GGG - chr3.4:75326969-75326988 None:intergenic 50.0%
TTCGACGGCTGATAAAACGC+CGG - chr3.4:75327345-75327364 None:intergenic 50.0%
TTGGAGGATCTGTGAAGACG+TGG + chr3.4:75327505-75327524 MS.gene074408:CDS 50.0%
! AACGTTGAAGCTGAGAGCAC+CGG + chr3.4:75327470-75327489 MS.gene074408:CDS 50.0%
! ACTTTTGCGGCGAGTGTAGT+TGG - chr3.4:75327022-75327041 None:intergenic 50.0%
! CGGCATTGAACTTGCGAGAT+CGG + chr3.4:75327401-75327420 MS.gene074408:CDS 50.0%
!! AGCGTTGGTGAATGCGGTTA+CGG + chr3.4:75327431-75327450 MS.gene074408:CDS 50.0%
!! TCAGAGAGCGTTGGTGAATG+CGG + chr3.4:75327425-75327444 MS.gene074408:CDS 50.0%
AGGTTTGATCCTTGGGGAGC+TGG - chr3.4:75326963-75326982 None:intergenic 55.0%
CGTTGTGCATTCTCTGGTGG+TGG - chr3.4:75326994-75327013 None:intergenic 55.0%
! GAGATCGGATCAGAGAGCGT+TGG + chr3.4:75327416-75327435 MS.gene074408:CDS 55.0%
!! CATTCTCTGGTGGTGGGATG+CGG - chr3.4:75326987-75327006 None:intergenic 55.0%
!! TGGAAGGTGGAGAGGCGTTA+GGG - chr3.4:75326903-75326922 None:intergenic 55.0%
!! TTGGAAGGTGGAGAGGCGTT+AGG - chr3.4:75326904-75326923 None:intergenic 55.0%
ACACTCGCCGCAAAAGTCGC+CGG + chr3.4:75327025-75327044 MS.gene074408:CDS 60.0%
ACGGCTGATAAAACGCCGGC+AGG - chr3.4:75327341-75327360 None:intergenic 60.0%
GACAGCAACAGCATCCGCAC+CGG - chr3.4:75327047-75327066 None:intergenic 60.0%
GGTGGATCAGTTAACGGCGC+AGG + chr3.4:75327560-75327579 MS.gene074408:CDS 60.0%
TCTCTGTAACGGCGTCCTGC+CGG + chr3.4:75327323-75327342 MS.gene074408:CDS 60.0%
! AGCGGTGTTGGAAGGTGGAG+AGG - chr3.4:75326911-75326930 None:intergenic 60.0%
! GATGGAGCGGTGTTGGAAGG+TGG - chr3.4:75326916-75326935 None:intergenic 60.0%
!! ATCCGCACCGGCGACTTTTG+CGG - chr3.4:75327035-75327054 None:intergenic 60.0%
GTGCTCATGGCCATCGCTGC+CGG + chr3.4:75327102-75327121 MS.gene074408:CDS 65.0%
TATGTTCCGGACGGCGGCGA+TGG - chr3.4:75326934-75326953 None:intergenic 65.0%
! GGCGATGGAGCGGTGTTGGA+AGG - chr3.4:75326919-75326938 None:intergenic 65.0%
!! CTCTGGTGGTGGGATGCGGT+AGG - chr3.4:75326983-75327002 None:intergenic 65.0%
CGCCGCAAAAGTCGCCGGTG+CGG + chr3.4:75327030-75327049 MS.gene074408:CDS 70.0%
ACCGCTCCATCGCCGCCGTC+CGG + chr3.4:75326925-75326944 MS.gene074408:CDS 75.0%
CGGCGGCGATGGAGCGGTGT+TGG - chr3.4:75326923-75326942 None:intergenic 75.0%
TCCGGACGGCGGCGATGGAG+CGG - chr3.4:75326929-75326948 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 75326883 75327632 75326883 ID=MS.gene074408
chr3.4 mRNA 75326883 75327632 75326883 ID=MS.gene074408.t1;Parent=MS.gene074408
chr3.4 exon 75326883 75327632 75326883 ID=MS.gene074408.t1.exon1;Parent=MS.gene074408.t1
chr3.4 CDS 75326883 75327632 75326883 ID=cds.MS.gene074408.t1;Parent=MS.gene074408.t1
Gene Sequence

>MS.gene074408

ATGGCTGATCGTGTTCACCCTAACGCCTCTCCACCTTCCAACACCGCTCCATCGCCGCCGTCCGGAACATATGTCATCCAGCTCCCCAAGGATCAAACCTACCGCATCCCACCACCAGAGAATGCACAACGTTACGCCAACTACACTCGCCGCAAAAGTCGCCGGTGCGGATGCTGTTGCTGTCTTTGCTGGTTCATCGGTATTTTATTTACTCTGATCGTGCTCATGGCCATCGCTGCCGGTGTTTTTTACTTGATCGTTCGTCCGGAAGCACCGAAATACACAATCGATCGTGTCTCCGTCAAGGGAATGAACCTAACATCTTTATCTACTATTTCACCCGAGTTTGATGTTTCTGTTAAAGCTGATAACGGTAACAAAAAGATCGGAATCTACTACGAAAGCGATAGCAGCGTTGAAATGTTTTACAGAGGCGTTAGTCTCTGTAACGGCGTCCTGCCGGCGTTTTATCAGCCGTCGAATAACGTGACGGTGTTTCAGACGGTGTTGAAAGGTAACGGCATTGAACTTGCGAGATCGGATCAGAGAGCGTTGGTGAATGCGGTTACGGAACGAAGCGTGCCGTTAACGTTGAAGCTGAGAGCACCGGTGAAGATTAAAGTTGGAGGATCTGTGAAGACGTGGAAGGTTAGAGTTAAGGTTGACTGTGATCTAACGGTGGATCAGTTAACGGCGCAGGCGAAGATTGTTGACAAGGATTGTAGTTATGAATTTGATCTTTGGTTATGA

Protein sequence

>MS.gene074408.t1

MADRVHPNASPPSNTAPSPPSGTYVIQLPKDQTYRIPPPENAQRYANYTRRKSRRCGCCCCLCWFIGILFTLIVLMAIAAGVFYLIVRPEAPKYTIDRVSVKGMNLTSLSTISPEFDVSVKADNGNKKIGIYYESDSSVEMFYRGVSLCNGVLPAFYQPSNNVTVFQTVLKGNGIELARSDQRALVNAVTERSVPLTLKLRAPVKIKVGGSVKTWKVRVKVDCDLTVDQLTAQAKIVDKDCSYEFDLWL