AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene055478

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055478.t1 MTR_4g129820 99.768 431 1 0 1 431 1 431 0.0 890
MS.gene055478.t1 MTR_4g024600 67.593 432 130 4 6 430 8 436 0.0 591
MS.gene055478.t1 MTR_0354s0040 67.849 423 122 4 13 430 4 417 0.0 587
MS.gene055478.t1 MTR_3g084090 65.187 428 135 5 13 427 5 431 0.0 553
MS.gene055478.t1 MTR_4g102270 62.238 429 150 3 4 426 2 424 0.0 551
MS.gene055478.t1 MTR_5g011940 69.972 363 107 1 73 433 66 428 0.0 529
MS.gene055478.t1 MTR_0034s0060 28.144 334 211 9 96 422 138 449 2.31e-23 103
MS.gene055478.t1 MTR_2g032100 26.629 353 223 12 89 421 65 401 3.68e-23 101
MS.gene055478.t1 MTR_2g073240 27.193 342 208 9 96 424 20 333 3.68e-22 99.8
MS.gene055478.t1 MTR_8g099535 28.022 364 211 15 96 431 5 345 5.81e-22 96.7
MS.gene055478.t1 MTR_1g009600 26.100 341 209 9 96 422 6 317 1.02e-21 98.2
MS.gene055478.t1 MTR_5g009170 27.401 354 222 13 96 429 48 386 1.54e-20 93.2
MS.gene055478.t1 MTR_2g032100 30.612 196 132 3 89 282 65 258 3.55e-20 90.9
MS.gene055478.t1 MTR_1g088480 27.195 353 202 15 84 419 98 412 5.51e-20 92.0
MS.gene055478.t1 MTR_8g089180 25.691 362 230 13 90 429 66 410 1.74e-19 90.5
MS.gene055478.t1 MTR_8g089180 25.691 362 230 13 90 429 66 410 1.74e-19 90.5
MS.gene055478.t1 MTR_4g063600 26.807 332 203 13 92 415 29 328 3.49e-19 88.6
MS.gene055478.t1 MTR_2g096660 26.567 335 200 13 92 415 35 334 1.00e-18 87.4
MS.gene055478.t1 MTR_5g009160 26.536 358 220 14 96 429 5 343 1.62e-18 86.7
MS.gene055478.t1 MTR_4g063600 27.108 332 199 14 92 415 29 325 1.28e-17 84.0
MS.gene055478.t1 MTR_4g120030 24.096 415 247 16 27 419 37 405 3.20e-17 83.6
MS.gene055478.t1 MTR_8g096880 25.862 348 218 12 97 421 8 338 5.99e-17 82.0
MS.gene055478.t1 MTR_8g096880 26.727 333 204 12 97 406 8 323 1.81e-16 80.5
MS.gene055478.t1 MTR_4g134490 24.661 369 230 12 79 421 11 357 2.35e-16 80.5
MS.gene055478.t1 MTR_8g096880 34.356 163 101 4 97 256 8 167 1.03e-15 77.8
MS.gene055478.t1 MTR_4g134490 24.928 349 224 11 79 406 11 342 1.47e-15 78.2
MS.gene055478.t1 MTR_7g115080 24.189 339 213 12 95 421 63 369 7.70e-13 70.1
MS.gene055478.t1 MTR_7g115080 23.599 339 215 11 95 421 29 335 8.36e-13 70.1
MS.gene055478.t1 MTR_7g115080 23.599 339 215 11 95 421 42 348 9.57e-13 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055478.t1 AT4G30440 85.579 423 57 1 6 428 3 421 0.0 749
MS.gene055478.t1 AT1G02000 69.231 429 124 5 5 429 2 426 0.0 600
MS.gene055478.t1 AT4G00110 68.650 437 119 5 1 430 1 426 0.0 597
MS.gene055478.t1 AT2G45310 67.051 434 124 5 5 426 2 428 0.0 575
MS.gene055478.t1 AT3G23820 72.521 353 97 0 75 427 92 444 0.0 547
MS.gene055478.t1 AT4G12250 65.429 431 135 4 5 426 2 427 0.0 539
MS.gene055478.t1 AT4G23920 29.178 353 200 13 96 421 4 333 8.35e-25 105
MS.gene055478.t1 AT3G14790 27.246 334 214 9 96 422 8 319 8.66e-23 101
MS.gene055478.t1 AT3G14790 27.246 334 214 9 96 422 11 322 9.89e-23 101
MS.gene055478.t1 AT1G78570 27.844 334 212 10 96 422 8 319 5.91e-22 99.0
MS.gene055478.t1 AT4G10960 28.691 359 209 16 96 428 5 342 4.10e-21 94.4
MS.gene055478.t1 AT1G53500 27.246 334 214 9 96 422 10 321 4.52e-21 96.3
MS.gene055478.t1 AT1G30620 25.691 362 234 12 90 431 66 412 8.73e-21 94.4
MS.gene055478.t1 AT1G30620 25.691 362 234 12 90 431 66 412 8.73e-21 94.4
MS.gene055478.t1 AT1G30620 25.691 362 234 12 90 431 66 412 8.73e-21 94.4
MS.gene055478.t1 AT1G30620 25.691 362 234 12 90 431 66 412 8.73e-21 94.4
MS.gene055478.t1 AT3G62830 26.765 340 208 12 91 422 115 421 8.79e-21 94.7
MS.gene055478.t1 AT3G62830 26.765 340 208 12 91 422 115 421 8.79e-21 94.7
MS.gene055478.t1 AT1G30620 25.691 362 234 12 90 431 65 411 9.78e-21 94.4
MS.gene055478.t1 AT2G47650 26.844 339 197 13 95 420 121 421 6.50e-20 92.0
MS.gene055478.t1 AT2G28760 26.866 335 199 13 92 415 28 327 9.96e-20 90.5
MS.gene055478.t1 AT2G28760 26.866 335 199 13 92 415 28 327 9.96e-20 90.5
MS.gene055478.t1 AT2G28760 26.866 335 199 13 92 415 28 327 9.96e-20 90.5
MS.gene055478.t1 AT2G28760 26.866 335 199 13 92 415 59 358 1.62e-19 90.1
MS.gene055478.t1 AT3G53520 25.444 338 201 12 95 419 120 419 1.99e-19 90.5
MS.gene055478.t1 AT1G12780 26.857 350 214 13 96 421 8 339 2.11e-19 89.4
MS.gene055478.t1 AT1G64440 26.099 364 222 12 96 433 4 346 5.16e-19 88.2
MS.gene055478.t1 AT5G44480 25.356 351 226 12 91 421 67 401 8.25e-19 88.6
MS.gene055478.t1 AT5G44480 25.356 351 226 12 91 421 67 401 8.25e-19 88.6
MS.gene055478.t1 AT5G44480 25.356 351 226 12 91 421 67 401 8.25e-19 88.6
MS.gene055478.t1 AT5G44480 25.356 351 226 12 91 421 91 425 1.36e-18 87.8
MS.gene055478.t1 AT1G30620 29.744 195 133 3 90 282 66 258 1.71e-18 85.9
MS.gene055478.t1 AT4G20460 25.620 363 231 14 91 431 66 411 2.56e-18 87.0
MS.gene055478.t1 AT2G47650 26.239 343 200 14 95 420 121 427 4.72e-18 86.3
MS.gene055478.t1 AT1G63180 27.089 347 217 13 96 421 8 339 8.59e-18 84.7
MS.gene055478.t1 AT3G53520 25.148 338 200 13 95 419 120 417 4.65e-17 83.2
MS.gene055478.t1 AT1G63180 28.231 294 180 11 96 370 8 289 4.98e-16 79.0
MS.gene055478.t1 AT3G53520 24.286 350 213 13 95 419 120 442 9.97e-16 79.3
MS.gene055478.t1 AT3G53520 27.731 238 148 8 95 325 120 340 6.03e-15 76.3
MS.gene055478.t1 AT5G28840 22.832 346 210 12 95 421 28 335 1.57e-11 66.2
MS.gene055478.t1 AT5G28840 22.832 346 210 12 95 421 28 335 1.57e-11 66.2

Find 113 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGTGGTATCTTTCTTATTA+TGG 0.181735 8.3:-1729709 MS.gene055478:CDS
GCTTCTTCAAGTTCGGTTTA+TGG 0.210656 8.3:-1730312 MS.gene055478:CDS
ATCCTCAACCGTCGATTGTT+TGG 0.264214 8.3:-1730335 MS.gene055478:CDS
CACACTTATAATCACATTTA+CGG 0.269492 8.3:-1730195 MS.gene055478:CDS
TATCTTTCTTATTATGGTTA+TGG 0.271440 8.3:-1729703 MS.gene055478:CDS
TCCTCAACCGTCGATTGTTT+GGG 0.276207 8.3:-1730334 MS.gene055478:CDS
GCCGGTATCTCTCCGCTTCA+TGG 0.283731 8.3:-1730767 MS.gene055478:CDS
ACCGGCGCAGCAGGTTTCAT+TGG 0.292862 8.3:-1730669 MS.gene055478:CDS
CGTCAGGTTCACCGTTGTTT+CGG 0.306919 8.3:-1730879 MS.gene055478:CDS
AATGCCATCATCATCATCTT+TGG 0.333111 8.3:-1730967 MS.gene055478:CDS
TTTCAAGGCGTCGTTGATTC+CGG 0.335632 8.3:-1730792 MS.gene055478:CDS
CGGTTTATCGGGGTAAGAAT+CGG 0.338698 8.3:-1730065 MS.gene055478:CDS
GTCTTTCAATCACTGGTTTA+CGG 0.353235 8.3:-1730173 MS.gene055478:CDS
ACCGTATCGGATCTTTAATC+TGG 0.354061 8.3:-1729929 MS.gene055478:CDS
TTCAAGCACCATGTTCTTGT+GGG 0.364536 8.3:-1730853 MS.gene055478:CDS
TTCACTAGAAATATGCTTCA+AGG 0.375977 8.3:-1730099 MS.gene055478:CDS
ACTTCGGCTAGAAGAGAATT+CGG 0.384585 8.3:-1729778 MS.gene055478:CDS
GCTTCAAGGAAAACCGATTA+CGG 0.396931 8.3:-1730085 MS.gene055478:CDS
CCCACTACTGATATTCAAAC+CGG 0.398293 8.3:-1729748 MS.gene055478:CDS
TTACCCCGATAAACCGTAAT+CGG 0.399655 8.3:+1730072 None:intergenic
GTTCTTGTCACCGGCGCAGC+AGG 0.407852 8.3:-1730678 MS.gene055478:CDS
CCGGCGCAGCAGGTTTCATT+GGG 0.408918 8.3:-1730668 MS.gene055478:CDS
GTTGGATCATTGGATACTTC+TGG 0.415498 8.3:-1729991 MS.gene055478:CDS
GAAAACCGATTACGGTTTAT+CGG 0.416082 8.3:-1730077 MS.gene055478:CDS
TGTTTGGGCTTCTTCAAGTT+CGG 0.422515 8.3:-1730319 MS.gene055478:CDS
CCGGTATCTCTCCGCTTCAT+GGG 0.427134 8.3:-1730766 MS.gene055478:CDS
GGTTTATCGGGGTAAGAATC+GGG 0.437179 8.3:-1730064 MS.gene055478:CDS
CTTCAAGCACCATGTTCTTG+TGG 0.437354 8.3:-1730854 MS.gene055478:CDS
GAAGGGATGTGTTGGATCAT+TGG 0.440266 8.3:-1730001 MS.gene055478:CDS
CCCAATGAAACCTGCTGCGC+CGG 0.445644 8.3:+1730668 None:intergenic
AAAACCGATTACGGTTTATC+GGG 0.446835 8.3:-1730076 MS.gene055478:CDS
CTTCATGGGGTGGAATTCAA+TGG 0.449305 8.3:-1730752 MS.gene055478:CDS
TATACTTGAGCGACTCTTGA+AGG 0.450751 8.3:-1729869 MS.gene055478:CDS
CCCATGAAGCGGAGAGATAC+CGG 0.457700 8.3:+1730766 None:intergenic
CGGTTGATGCGATAAGCAAA+AGG 0.460035 8.3:+1730829 None:intergenic
ATCGGATCGGACCGATCAAC+CGG 0.460933 8.3:-1730262 MS.gene055478:CDS
GAACTGACATGCCGCCTTGA+CGG 0.461870 8.3:+1730697 None:intergenic
AAGAATATTGTAGACATGCC+TGG 0.472731 8.3:-1729835 MS.gene055478:CDS
GATACTTCTGGTAAGAGTAC+TGG 0.473936 8.3:-1729979 MS.gene055478:CDS
GTCCGATCCGATTCAGAAAA+CGG 0.482746 8.3:+1730273 None:intergenic
ATTTACGGTCTTTCAATCAC+TGG 0.485594 8.3:-1730180 MS.gene055478:CDS
AAGGGAACATCACCATTTCC+AGG 0.495063 8.3:+1729817 None:intergenic
CTTCGGCTAGAAGAGAATTC+GGG 0.501557 8.3:-1729777 MS.gene055478:CDS
TTTCACTGTTTATGGACCAT+GGG 0.501706 8.3:-1730148 MS.gene055478:CDS
ATTGTAGACATGCCTGGAAA+TGG 0.502297 8.3:-1729829 MS.gene055478:CDS
ATATAAACTCGCCGGTTGAT+CGG 0.504741 8.3:+1730251 None:intergenic
TTCATGGGGTGGAATTCAAT+GGG 0.507931 8.3:-1730751 MS.gene055478:CDS
TCTCTTGCTTTGAAAAGAAG+AGG 0.508211 8.3:-1730636 MS.gene055478:CDS
CCGTATCGGATCTTTAATCT+GGG 0.514097 8.3:-1729928 MS.gene055478:CDS
AACGTGGAGCAGCACCGTAT+CGG 0.516784 8.3:-1729942 MS.gene055478:CDS
ACAAGTGTCGGCACCGTCAC+CGG 0.517543 8.3:+1729895 None:intergenic
AGCGGAGAGATACCGGCTAC+CGG 0.518013 8.3:+1730773 None:intergenic
GGGGAATACTTCGCCGGTGA+CGG 0.520167 8.3:-1729908 MS.gene055478:CDS
CTTCACTATATCGTCAATGT+AGG 0.520270 8.3:+1730020 None:intergenic
AGAAGAGGTGATGGTGTCGT+TGG 0.521236 8.3:-1730621 MS.gene055478:CDS
CTGGAGTGGATGGGTAGAGT+TGG 0.525095 8.3:+1730940 None:intergenic
TAATCTGGGGAATACTTCGC+CGG 0.527767 8.3:-1729914 MS.gene055478:CDS
TCTTCTTGAAGCTTGCAAAA+CGG 0.530117 8.3:-1730361 MS.gene055478:CDS
TTGCTGTTAACATATGACAT+AGG 0.539948 8.3:+1730402 None:intergenic
CAACTCTACCCATCCACTCC+AGG 0.542396 8.3:-1730939 MS.gene055478:CDS
TTCAAGAAGAGTGACTAAAC+CGG 0.543596 8.3:+1730374 None:intergenic
TATCTCTCCGCTTCATGGGG+TGG 0.546092 8.3:-1730762 MS.gene055478:CDS
TTCTGGTAAGAGTACTGGAT+CGG 0.547457 8.3:-1729974 MS.gene055478:CDS
GCCCAAACAATCGACGGTTG+AGG 0.547804 8.3:+1730333 None:intergenic
GCGTCGTTGATTCCGGTAGC+CGG 0.549486 8.3:-1730785 MS.gene055478:CDS
GGTTGATGCGATAAGCAAAA+GGG 0.551156 8.3:+1730830 None:intergenic
GTGATATTCGCATGAGTGAA+GGG 0.552540 8.3:+1729799 None:intergenic
GATATAGTGAAGGGATGTGT+TGG 0.556067 8.3:-1730009 MS.gene055478:CDS
TTTCAAAGCAAGAGAAACAT+GGG 0.556424 8.3:+1730644 None:intergenic
CCACGGTCTAATGAACCGTC+AGG 0.559363 8.3:-1730895 MS.gene055478:CDS
CCGGTTTGAATATCAGTAGT+GGG 0.560234 8.3:+1729748 None:intergenic
GCCATGTCTGGTCTTCCCCA+TGG 0.560372 8.3:+1730132 None:intergenic
CCCGGTTTGAATATCAGTAG+TGG 0.562993 8.3:+1729747 None:intergenic
GGTTTATGGTTTAAACGAGA+AGG 0.563313 8.3:-1730298 MS.gene055478:CDS
TCTGGTAAGAGTACTGGATC+GGG 0.564479 8.3:-1729973 MS.gene055478:CDS
AGCTGGTGTGAGATACGCTA+TGG 0.564884 8.3:-1730430 MS.gene055478:CDS
ATGCATCTCGCAGCTCAAGC+TGG 0.567280 8.3:-1730447 MS.gene055478:CDS
GGCATGTCAGTTCTTGTCAC+CGG 0.570857 8.3:-1730687 MS.gene055478:CDS
TATGCAGCGACGAAGAAAGC+AGG 0.574218 8.3:-1730231 MS.gene055478:CDS
CAAAAGGGCCCACAAGAACA+TGG 0.581018 8.3:+1730845 None:intergenic
TTGAAAAGTGACGTAAGAAA+CGG 0.582579 8.3:+1730809 None:intergenic
GTCGCTGCATATAAACTCGC+CGG 0.586523 8.3:+1730243 None:intergenic
TTGACGGTGGATTTGAGCGG+AGG 0.587662 8.3:+1730713 None:intergenic
GGTAAGAGTACTGGATCGGG+TGG 0.590345 8.3:-1729970 MS.gene055478:CDS
CACTCATGCGAATATCACTT+CGG 0.595164 8.3:-1729794 MS.gene055478:CDS
CCCAGATTAAAGATCCGATA+CGG 0.595228 8.3:+1729928 None:intergenic
CGAAACAACGGTGAACCTGA+CGG 0.595789 8.3:+1730880 None:intergenic
TATGTTAACAGCAACATCGC+CGG 0.597339 8.3:-1730393 MS.gene055478:CDS
CGGTATCTCTCCGCTTCATG+GGG 0.598560 8.3:-1730765 MS.gene055478:CDS
AGTGATATTCGCATGAGTGA+AGG 0.599504 8.3:+1729798 None:intergenic
TTGAATTCCACCCCATGAAG+CGG 0.604390 8.3:+1730755 None:intergenic
CTACATTGACGATATAGTGA+AGG 0.605438 8.3:-1730019 MS.gene055478:CDS
TCCGCTCAAATCCACCGTCA+AGG 0.609000 8.3:-1730711 MS.gene055478:CDS
TGAGCGACTCTTGAAGGTGA+AGG 0.622782 8.3:-1729863 MS.gene055478:CDS
CCACTACTGATATTCAAACC+GGG 0.625227 8.3:-1729747 MS.gene055478:CDS
TACATTGACGATATAGTGAA+GGG 0.627843 8.3:-1730018 MS.gene055478:CDS
CATGCACGATGAAAACGCCC+CGG 0.632042 8.3:+1730532 None:intergenic
TCAAGTATACTCACAAGTGT+CGG 0.632370 8.3:+1729883 None:intergenic
GCCTTGACGGTGGATTTGAG+CGG 0.637649 8.3:+1730710 None:intergenic
CTGACATGCCGCCTTGACGG+TGG 0.641878 8.3:+1730700 None:intergenic
GCTTTGAAAAGAAGAGGTGA+TGG 0.645226 8.3:-1730630 MS.gene055478:CDS
GGATCGGGTGGGAAGAAACG+TGG 0.648196 8.3:-1729958 MS.gene055478:CDS
TTCAAGATCGAAAGAAACCA+CGG 0.649323 8.3:-1730912 MS.gene055478:CDS
ACCATGGGGAAGACCAGACA+TGG 0.649667 8.3:-1730133 MS.gene055478:CDS
TTCACTGTTTATGGACCATG+GGG 0.649938 8.3:-1730147 MS.gene055478:CDS
TCTTCCAAAGATGATGATGA+TGG 0.654769 8.3:+1730963 None:intergenic
TGCTTGAAGAACCGAAACAA+CGG 0.657956 8.3:+1730868 None:intergenic
GAAGAAGCCCAAACAATCGA+CGG 0.665669 8.3:+1730327 None:intergenic
GTAAGAGTACTGGATCGGGT+GGG 0.675997 8.3:-1729969 MS.gene055478:CDS
AAACCGATTACGGTTTATCG+GGG 0.681675 8.3:-1730075 MS.gene055478:CDS
CGTATCGGATCTTTAATCTG+GGG 0.681906 8.3:-1729927 MS.gene055478:CDS
CCTGACGGTTCATTAGACCG+TGG 0.701311 8.3:+1730895 None:intergenic
GCTCAAATCCACCGTCAAGG+CGG 0.724590 8.3:-1730708 MS.gene055478:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCTTTCTTATTATGGTTA+TGG - chr8.3:1730950-1730969 MS.gene055478:CDS 20.0%
! AAGTGGTATCTTTCTTATTA+TGG - chr8.3:1730944-1730963 MS.gene055478:CDS 25.0%
! CACACTTATAATCACATTTA+CGG - chr8.3:1730458-1730477 MS.gene055478:CDS 25.0%
AAAGTAAAAATAGCCATGTC+TGG + chr8.3:1730536-1730555 None:intergenic 30.0%
TACATTGACGATATAGTGAA+GGG - chr8.3:1730635-1730654 MS.gene055478:CDS 30.0%
TTCACTAGAAATATGCTTCA+AGG - chr8.3:1730554-1730573 MS.gene055478:CDS 30.0%
TTGAAAAGTGACGTAAGAAA+CGG + chr8.3:1729847-1729866 None:intergenic 30.0%
TTTCAAAGCAAGAGAAACAT+GGG + chr8.3:1730012-1730031 None:intergenic 30.0%
! TTACGGTTTTTCACTGTTTA+TGG - chr8.3:1730497-1730516 MS.gene055478:CDS 30.0%
! TTGCTGTTAACATATGACAT+AGG + chr8.3:1730254-1730273 None:intergenic 30.0%
! TTTTCAAAGCAAGAGAAACA+TGG + chr8.3:1730013-1730032 None:intergenic 30.0%
!!! TTTCGAGCTTTTTTCAATGA+AGG + chr8.3:1730086-1730105 None:intergenic 30.0%
AAAACCGATTACGGTTTATC+GGG - chr8.3:1730577-1730596 MS.gene055478:CDS 35.0%
AAGAATATTGTAGACATGCC+TGG - chr8.3:1730818-1730837 MS.gene055478:CDS 35.0%
ATTTACGGTCTTTCAATCAC+TGG - chr8.3:1730473-1730492 MS.gene055478:CDS 35.0%
CTACATTGACGATATAGTGA+AGG - chr8.3:1730634-1730653 MS.gene055478:CDS 35.0%
CTTCACTATATCGTCAATGT+AGG + chr8.3:1730636-1730655 None:intergenic 35.0%
GAAAACCGATTACGGTTTAT+CGG - chr8.3:1730576-1730595 MS.gene055478:CDS 35.0%
GGTTGAAAAAATTCGTCAAG+TGG - chr8.3:1730927-1730946 MS.gene055478:CDS 35.0%
GGTTTATGGTTTAAACGAGA+AGG - chr8.3:1730355-1730374 MS.gene055478:CDS 35.0%
TCAAGTATACTCACAAGTGT+CGG + chr8.3:1730773-1730792 None:intergenic 35.0%
TCTTCTTGAAGCTTGCAAAA+CGG - chr8.3:1730292-1730311 MS.gene055478:CDS 35.0%
TTCAAGAAGAGTGACTAAAC+CGG + chr8.3:1730282-1730301 None:intergenic 35.0%
TTCAAGATCGAAAGAAACCA+CGG - chr8.3:1729741-1729760 MS.gene055478:CDS 35.0%
TTTCACTGTTTATGGACCAT+GGG - chr8.3:1730505-1730524 MS.gene055478:CDS 35.0%
! AAAAGCTCTTTTATCAAGCC+GGG - chr8.3:1730103-1730122 MS.gene055478:CDS 35.0%
! CTTTCGATCTTGAATTTTCC+TGG + chr8.3:1729735-1729754 None:intergenic 35.0%
! GAAAAGCTCTTTTATCAAGC+CGG - chr8.3:1730102-1730121 MS.gene055478:CDS 35.0%
! TCTTCCAAAGATGATGATGA+TGG + chr8.3:1729693-1729712 None:intergenic 35.0%
! TTTTCACTGTTTATGGACCA+TGG - chr8.3:1730504-1730523 MS.gene055478:CDS 35.0%
!! GTCTTTCAATCACTGGTTTA+CGG - chr8.3:1730480-1730499 MS.gene055478:CDS 35.0%
!! TCTCTTGCTTTGAAAAGAAG+AGG - chr8.3:1730017-1730036 MS.gene055478:CDS 35.0%
!!! CTTGACGAATTTTTTCAACC+CGG + chr8.3:1730927-1730946 None:intergenic 35.0%
!!! GTTTCTTACGTCACTTTTCA+AGG - chr8.3:1729846-1729865 MS.gene055478:CDS 35.0%
AAACCGATTACGGTTTATCG+GGG - chr8.3:1730578-1730597 MS.gene055478:CDS 40.0%
ACCGTATCGGATCTTTAATC+TGG - chr8.3:1730724-1730743 MS.gene055478:CDS 40.0%
ACTTCGGCTAGAAGAGAATT+CGG - chr8.3:1730875-1730894 MS.gene055478:CDS 40.0%
AGTGATATTCGCATGAGTGA+AGG + chr8.3:1730858-1730877 None:intergenic 40.0%
ATTGTAGACATGCCTGGAAA+TGG - chr8.3:1730824-1730843 MS.gene055478:CDS 40.0%
CACTCATGCGAATATCACTT+CGG - chr8.3:1730859-1730878 MS.gene055478:CDS 40.0%
CCACTACTGATATTCAAACC+GGG - chr8.3:1730906-1730925 MS.gene055478:CDS 40.0%
CCCACTACTGATATTCAAAC+CGG - chr8.3:1730905-1730924 MS.gene055478:CDS 40.0%
CCCAGATTAAAGATCCGATA+CGG + chr8.3:1730728-1730747 None:intergenic 40.0%
CCGTATCGGATCTTTAATCT+GGG - chr8.3:1730725-1730744 MS.gene055478:CDS 40.0%
CGTATCGGATCTTTAATCTG+GGG - chr8.3:1730726-1730745 MS.gene055478:CDS 40.0%
GATATAGTGAAGGGATGTGT+TGG - chr8.3:1730644-1730663 MS.gene055478:CDS 40.0%
GCTTCAAGGAAAACCGATTA+CGG - chr8.3:1730568-1730587 MS.gene055478:CDS 40.0%
GTGATATTCGCATGAGTGAA+GGG + chr8.3:1730857-1730876 None:intergenic 40.0%
GTTGGATCATTGGATACTTC+TGG - chr8.3:1730662-1730681 MS.gene055478:CDS 40.0%
TATACTTGAGCGACTCTTGA+AGG - chr8.3:1730784-1730803 MS.gene055478:CDS 40.0%
TATGTTAACAGCAACATCGC+CGG - chr8.3:1730260-1730279 MS.gene055478:CDS 40.0%
TGCTTGAAGAACCGAAACAA+CGG + chr8.3:1729788-1729807 None:intergenic 40.0%
TTACCCCGATAAACCGTAAT+CGG + chr8.3:1730584-1730603 None:intergenic 40.0%
TTCAAGCACCATGTTCTTGT+GGG - chr8.3:1729800-1729819 MS.gene055478:CDS 40.0%
TTCACTGTTTATGGACCATG+GGG - chr8.3:1730506-1730525 MS.gene055478:CDS 40.0%
TTCATGGGGTGGAATTCAAT+GGG - chr8.3:1729902-1729921 MS.gene055478:CDS 40.0%
! AAAGCTCTTTTATCAAGCCG+GGG - chr8.3:1730104-1730123 MS.gene055478:CDS 40.0%
! AGCAAAGCTTTTCGATGTTG+TGG - chr8.3:1730169-1730188 MS.gene055478:CDS 40.0%
! ATATAAACTCGCCGGTTGAT+CGG + chr8.3:1730405-1730424 None:intergenic 40.0%
! CCGGTTTGAATATCAGTAGT+GGG + chr8.3:1730908-1730927 None:intergenic 40.0%
! GAAGGTTCCGTTTTCTGAAT+CGG - chr8.3:1730373-1730392 MS.gene055478:CDS 40.0%
! GATACTTCTGGTAAGAGTAC+TGG - chr8.3:1730674-1730693 MS.gene055478:CDS 40.0%
! GATCTTGAATTTTCCTGGAG+TGG + chr8.3:1729730-1729749 None:intergenic 40.0%
! GCTTCTTCAAGTTCGGTTTA+TGG - chr8.3:1730341-1730360 MS.gene055478:CDS 40.0%
! GGTTGATGCGATAAGCAAAA+GGG + chr8.3:1729826-1729845 None:intergenic 40.0%
! TGAATTTTCCTGGAGTGGAT+GGG + chr8.3:1729725-1729744 None:intergenic 40.0%
! TGTTTGGGCTTCTTCAAGTT+CGG - chr8.3:1730334-1730353 MS.gene055478:CDS 40.0%
! TTCCGTTTTCTGAATCGGAT+CGG - chr8.3:1730378-1730397 MS.gene055478:CDS 40.0%
! TTGAATTTTCCTGGAGTGGA+TGG + chr8.3:1729726-1729745 None:intergenic 40.0%
!! GCTTTGAAAAGAAGAGGTGA+TGG - chr8.3:1730023-1730042 MS.gene055478:CDS 40.0%
!! TTCTGGTAAGAGTACTGGAT+CGG - chr8.3:1730679-1730698 MS.gene055478:CDS 40.0%
AAGGGAACATCACCATTTCC+AGG + chr8.3:1730839-1730858 None:intergenic 45.0%
CGGTTTATCGGGGTAAGAAT+CGG - chr8.3:1730588-1730607 MS.gene055478:CDS 45.0%
CTTCAAGCACCATGTTCTTG+TGG - chr8.3:1729799-1729818 MS.gene055478:CDS 45.0%
CTTCATGGGGTGGAATTCAA+TGG - chr8.3:1729901-1729920 MS.gene055478:CDS 45.0%
CTTCGGCTAGAAGAGAATTC+GGG - chr8.3:1730876-1730895 MS.gene055478:CDS 45.0%
GAAGAAGCCCAAACAATCGA+CGG + chr8.3:1730329-1730348 None:intergenic 45.0%
GAAGGGATGTGTTGGATCAT+TGG - chr8.3:1730652-1730671 MS.gene055478:CDS 45.0%
GGTTTATCGGGGTAAGAATC+GGG - chr8.3:1730589-1730608 MS.gene055478:CDS 45.0%
GTCCGATCCGATTCAGAAAA+CGG + chr8.3:1730383-1730402 None:intergenic 45.0%
TAATCTGGGGAATACTTCGC+CGG - chr8.3:1730739-1730758 MS.gene055478:CDS 45.0%
TTGAATTCCACCCCATGAAG+CGG + chr8.3:1729901-1729920 None:intergenic 45.0%
! ATCCTCAACCGTCGATTGTT+TGG - chr8.3:1730318-1730337 MS.gene055478:CDS 45.0%
! CCCGGTTTGAATATCAGTAG+TGG + chr8.3:1730909-1730928 None:intergenic 45.0%
! CGGTTGATGCGATAAGCAAA+AGG + chr8.3:1729827-1729846 None:intergenic 45.0%
! TCCTCAACCGTCGATTGTTT+GGG - chr8.3:1730319-1730338 MS.gene055478:CDS 45.0%
!! TCTGGTAAGAGTACTGGATC+GGG - chr8.3:1730680-1730699 MS.gene055478:CDS 45.0%
!! TTTCAAGGCGTCGTTGATTC+CGG - chr8.3:1729861-1729880 MS.gene055478:CDS 45.0%
AGCTGGTGTGAGATACGCTA+TGG - chr8.3:1730223-1730242 MS.gene055478:CDS 50.0%
CAAAAGGGCCCACAAGAACA+TGG + chr8.3:1729811-1729830 None:intergenic 50.0%
CGAAACAACGGTGAACCTGA+CGG + chr8.3:1729776-1729795 None:intergenic 50.0%
CGTCAGGTTCACCGTTGTTT+CGG - chr8.3:1729774-1729793 MS.gene055478:CDS 50.0%
GGCATGTCAGTTCTTGTCAC+CGG - chr8.3:1729966-1729985 MS.gene055478:CDS 50.0%
GTCGCTGCATATAAACTCGC+CGG + chr8.3:1730413-1730432 None:intergenic 50.0%
TATGCAGCGACGAAGAAAGC+AGG - chr8.3:1730422-1730441 MS.gene055478:CDS 50.0%
TGAGCGACTCTTGAAGGTGA+AGG - chr8.3:1730790-1730809 MS.gene055478:CDS 50.0%
!! AGAAGAGGTGATGGTGTCGT+TGG - chr8.3:1730032-1730051 MS.gene055478:CDS 50.0%
!! GTAAGAGTACTGGATCGGGT+GGG - chr8.3:1730684-1730703 MS.gene055478:CDS 50.0%
AACGTGGAGCAGCACCGTAT+CGG - chr8.3:1730711-1730730 MS.gene055478:CDS 55.0%
ACCATGGGGAAGACCAGACA+TGG - chr8.3:1730520-1730539 MS.gene055478:CDS 55.0%
ATCGGATCGGACCGATCAAC+CGG - chr8.3:1730391-1730410 MS.gene055478:CDS 55.0%
ATGCATCTCGCAGCTCAAGC+TGG - chr8.3:1730206-1730225 MS.gene055478:CDS 55.0%
CAACTCTACCCATCCACTCC+AGG - chr8.3:1729714-1729733 MS.gene055478:CDS 55.0%
CATGCACGATGAAAACGCCC+CGG + chr8.3:1730124-1730143 None:intergenic 55.0%
CCACGGTCTAATGAACCGTC+AGG - chr8.3:1729758-1729777 MS.gene055478:CDS 55.0%
CCCATGAAGCGGAGAGATAC+CGG + chr8.3:1729890-1729909 None:intergenic 55.0%
CCGGTATCTCTCCGCTTCAT+GGG - chr8.3:1729887-1729906 MS.gene055478:CDS 55.0%
CCTGACGGTTCATTAGACCG+TGG + chr8.3:1729761-1729780 None:intergenic 55.0%
CGGTATCTCTCCGCTTCATG+GGG - chr8.3:1729888-1729907 MS.gene055478:CDS 55.0%
CTGGAGTGGATGGGTAGAGT+TGG + chr8.3:1729716-1729735 None:intergenic 55.0%
GAACTGACATGCCGCCTTGA+CGG + chr8.3:1729959-1729978 None:intergenic 55.0%
GCCCAAACAATCGACGGTTG+AGG + chr8.3:1730323-1730342 None:intergenic 55.0%
GCTCAAATCCACCGTCAAGG+CGG - chr8.3:1729945-1729964 MS.gene055478:CDS 55.0%
TATCTCTCCGCTTCATGGGG+TGG - chr8.3:1729891-1729910 MS.gene055478:CDS 55.0%
TCCGCTCAAATCCACCGTCA+AGG - chr8.3:1729942-1729961 MS.gene055478:CDS 55.0%
! GCCTTGACGGTGGATTTGAG+CGG + chr8.3:1729946-1729965 None:intergenic 55.0%
! TTGACGGTGGATTTGAGCGG+AGG + chr8.3:1729943-1729962 None:intergenic 55.0%
!! GGTAAGAGTACTGGATCGGG+TGG - chr8.3:1730683-1730702 MS.gene055478:CDS 55.0%
ACCGGCGCAGCAGGTTTCAT+TGG - chr8.3:1729984-1730003 MS.gene055478:CDS 60.0%
CCCAATGAAACCTGCTGCGC+CGG + chr8.3:1729988-1730007 None:intergenic 60.0%
CCGGCGCAGCAGGTTTCATT+GGG - chr8.3:1729985-1730004 MS.gene055478:CDS 60.0%
GCCGGTATCTCTCCGCTTCA+TGG - chr8.3:1729886-1729905 MS.gene055478:CDS 60.0%
GGATCGGGTGGGAAGAAACG+TGG - chr8.3:1730695-1730714 MS.gene055478:CDS 60.0%
GGGGAATACTTCGCCGGTGA+CGG - chr8.3:1730745-1730764 MS.gene055478:CDS 60.0%
! AGCGGAGAGATACCGGCTAC+CGG + chr8.3:1729883-1729902 None:intergenic 60.0%
! GCCATGTCTGGTCTTCCCCA+TGG + chr8.3:1730524-1730543 None:intergenic 60.0%
!! ACAAGTGTCGGCACCGTCAC+CGG + chr8.3:1730761-1730780 None:intergenic 60.0%
!! CGGGGCGTTTTCATCGTGCA+TGG - chr8.3:1730122-1730141 MS.gene055478:CDS 60.0%
!! GCGTCGTTGATTCCGGTAGC+CGG - chr8.3:1729868-1729887 MS.gene055478:CDS 60.0%
CTGACATGCCGCCTTGACGG+TGG + chr8.3:1729956-1729975 None:intergenic 65.0%
GTTCTTGTCACCGGCGCAGC+AGG - chr8.3:1729975-1729994 MS.gene055478:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 1729687 1730988 1729687 ID=MS.gene055478
chr8.3 mRNA 1729687 1730988 1729687 ID=MS.gene055478.t1;Parent=MS.gene055478
chr8.3 exon 1729687 1730988 1729687 ID=MS.gene055478.t1.exon1;Parent=MS.gene055478.t1
chr8.3 CDS 1729687 1730988 1729687 ID=cds.MS.gene055478.t1;Parent=MS.gene055478.t1
Gene Sequence

>MS.gene055478

ATGCCATCATCATCATCTTTGGAAGACCAACTCTACCCATCCACTCCAGGAAAATTCAAGATCGAAAGAAACCACGGTCTAATGAACCGTCAGGTTCACCGTTGTTTCGGTTCTTCAAGCACCATGTTCTTGTGGGCCCTTTTGCTTATCGCATCAACCGTTTCTTACGTCACTTTTCAAGGCGTCGTTGATTCCGGTAGCCGGTATCTCTCCGCTTCATGGGGTGGAATTCAATGGGAAAAACAAGTCAGAGCCTCCGCTCAAATCCACCGTCAAGGCGGCATGTCAGTTCTTGTCACCGGCGCAGCAGGTTTCATTGGGTCCCATGTTTCTCTTGCTTTGAAAAGAAGAGGTGATGGTGTCGTTGGACTTGATAACTTCAACGATTACTATGATCCTTCATTGAAAAAAGCTCGAAAAGCTCTTTTATCAAGCCGGGGCGTTTTCATCGTGCATGGTGATTTAAACGACGCGAAGCTATTAGCAAAGCTTTTCGATGTTGTGGCTTTTACTCATGTGATGCATCTCGCAGCTCAAGCTGGTGTGAGATACGCTATGGAGAATCCTATGTCATATGTTAACAGCAACATCGCCGGTTTAGTCACTCTTCTTGAAGCTTGCAAAACGGCGAATCCTCAACCGTCGATTGTTTGGGCTTCTTCAAGTTCGGTTTATGGTTTAAACGAGAAGGTTCCGTTTTCTGAATCGGATCGGACCGATCAACCGGCGAGTTTATATGCAGCGACGAAGAAAGCAGGTGAAGAAATCACTCACACTTATAATCACATTTACGGTCTTTCAATCACTGGTTTACGGTTTTTCACTGTTTATGGACCATGGGGAAGACCAGACATGGCTATTTTTACTTTCACTAGAAATATGCTTCAAGGAAAACCGATTACGGTTTATCGGGGTAAGAATCGGGTTGATCTGTCACGTGACTTCACCTACATTGACGATATAGTGAAGGGATGTGTTGGATCATTGGATACTTCTGGTAAGAGTACTGGATCGGGTGGGAAGAAACGTGGAGCAGCACCGTATCGGATCTTTAATCTGGGGAATACTTCGCCGGTGACGGTGCCGACACTTGTGAGTATACTTGAGCGACTCTTGAAGGTGAAGGCAAAGAAGAATATTGTAGACATGCCTGGAAATGGTGATGTTCCCTTCACTCATGCGAATATCACTTCGGCTAGAAGAGAATTCGGGTATAAACCCACTACTGATATTCAAACCGGGTTGAAAAAATTCGTCAAGTGGTATCTTTCTTATTATGGTTATGGAAAAACTCTAAATTAG
Protein sequence

>MS.gene055478.t1

MPSSSSLEDQLYPSTPGKFKIERNHGLMNRQVHRCFGSSSTMFLWALLLIASTVSYVTFQGVVDSGSRYLSASWGGIQWEKQVRASAQIHRQGGMSVLVTGAAGFIGSHVSLALKRRGDGVVGLDNFNDYYDPSLKKARKALLSSRGVFIVHGDLNDAKLLAKLFDVVAFTHVMHLAAQAGVRYAMENPMSYVNSNIAGLVTLLEACKTANPQPSIVWASSSSVYGLNEKVPFSESDRTDQPASLYAATKKAGEEITHTYNHIYGLSITGLRFFTVYGPWGRPDMAIFTFTRNMLQGKPITVYRGKNRVDLSRDFTYIDDIVKGCVGSLDTSGKSTGSGGKKRGAAPYRIFNLGNTSPVTVPTLVSILERLLKVKAKKNIVDMPGNGDVPFTHANITSARREFGYKPTTDIQTGLKKFVKWYLSYYGYGKTLN