AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08225

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08225.t1 MTR_7g085390 98.925 465 5 0 1 465 16 480 0.0 944
MS.gene08225.t1 MTR_6g032820 80.332 422 80 3 1 419 16 437 0.0 693
MS.gene08225.t1 MTR_4g112025 57.609 460 188 3 5 459 27 484 0.0 544
MS.gene08225.t1 MTR_1g053320 55.921 456 199 2 11 464 30 485 0.0 524
MS.gene08225.t1 MTR_8g092070 54.756 431 192 2 10 439 33 461 5.86e-174 501
MS.gene08225.t1 MTR_1g010120 55.824 455 192 5 10 459 25 475 7.74e-173 494
MS.gene08225.t1 MTR_3g108600 52.723 459 187 5 6 456 26 462 6.30e-158 456
MS.gene08225.t1 MTR_3g095050 50.985 457 218 6 10 464 33 485 3.76e-156 452
MS.gene08225.t1 MTR_2g027560 50.577 433 206 5 11 440 28 455 1.07e-154 448
MS.gene08225.t1 MTR_4g124440 51.562 448 208 5 10 453 27 469 2.84e-154 447
MS.gene08225.t1 MTR_4g134280 49.326 371 175 5 99 462 2 366 5.73e-123 363
MS.gene08225.t1 MTR_3g031770 44.495 436 234 7 8 442 24 452 3.15e-118 355
MS.gene08225.t1 MTR_3g116510 33.713 439 265 18 11 435 28 454 3.82e-61 206
MS.gene08225.t1 MTR_1g031900 32.648 438 266 16 11 435 24 445 6.61e-61 207
MS.gene08225.t1 MTR_1g031900 32.648 438 266 16 11 435 24 445 1.66e-60 206
MS.gene08225.t1 MTR_4g069940 32.886 447 262 18 11 435 29 459 5.58e-58 198
MS.gene08225.t1 MTR_7g026340 34.537 443 255 20 11 435 23 448 3.53e-57 196
MS.gene08225.t1 MTR_5g085580 31.906 467 257 18 8 435 22 466 4.14e-57 196
MS.gene08225.t1 MTR_4g076255 34.862 327 192 9 8 331 22 330 3.91e-56 189
MS.gene08225.t1 MTR_6g088885 31.011 445 271 17 5 427 21 451 7.56e-56 194
MS.gene08225.t1 MTR_4g088155 30.580 448 275 19 11 435 23 457 7.71e-55 190
MS.gene08225.t1 MTR_4g076470 34.146 328 199 8 1 327 13 324 1.05e-53 183
MS.gene08225.t1 MTR_1g077990 34.731 334 197 11 8 332 28 349 1.49e-53 185
MS.gene08225.t1 MTR_4g076490 35.077 325 193 9 11 333 97 405 2.70e-53 184
MS.gene08225.t1 MTR_1g056640 30.277 469 273 19 8 435 35 490 7.65e-53 185
MS.gene08225.t1 MTR_3g065460 30.023 443 271 16 11 435 24 445 1.31e-52 184
MS.gene08225.t1 MTR_4g076430 32.733 333 207 8 1 332 13 329 5.76e-52 178
MS.gene08225.t1 MTR_2g034480 32.335 334 214 6 11 342 27 350 7.45e-52 179
MS.gene08225.t1 MTR_1g052430 33.851 322 197 9 10 327 31 340 1.58e-51 179
MS.gene08225.t1 MTR_4g076440 31.832 333 210 8 1 332 13 329 2.26e-51 177
MS.gene08225.t1 MTR_4g010200 34.940 332 195 12 12 333 34 354 5.38e-51 177
MS.gene08225.t1 MTR_1g022160 29.806 463 275 17 11 435 24 474 5.79e-51 180
MS.gene08225.t1 MTR_1g007810 35.435 333 187 14 8 325 13 332 5.82e-51 177
MS.gene08225.t1 MTR_4g083500 28.842 475 292 15 4 449 37 494 2.07e-50 179
MS.gene08225.t1 MTR_1g022160 29.718 461 274 17 11 433 24 472 2.92e-50 178
MS.gene08225.t1 MTR_2g034440 33.645 321 200 7 11 328 22 332 6.80e-50 173
MS.gene08225.t1 MTR_2g034470 33.131 329 204 7 11 335 30 346 1.42e-49 173
MS.gene08225.t1 MTR_1g022160 29.670 455 270 17 11 427 24 466 3.21e-49 175
MS.gene08225.t1 MTR_7g081350 32.416 327 201 11 8 329 24 335 3.24e-49 171
MS.gene08225.t1 MTR_4g114850 32.530 332 203 11 12 333 27 347 5.56e-49 172
MS.gene08225.t1 MTR_4g114850 32.530 332 203 11 12 333 27 347 9.41e-49 171
MS.gene08225.t1 MTR_4g114850 32.530 332 203 11 12 333 27 347 9.52e-49 171
MS.gene08225.t1 MTR_2g034470 33.758 314 192 7 26 335 10 311 1.29e-48 169
MS.gene08225.t1 MTR_3g080410 34.969 326 187 12 9 322 24 336 1.70e-48 170
MS.gene08225.t1 MTR_5g078200 29.452 438 280 14 11 435 23 444 1.99e-48 176
MS.gene08225.t1 MTR_8g012400 29.705 441 281 17 12 435 30 458 3.35e-48 172
MS.gene08225.t1 MTR_4g076570 32.326 331 207 9 8 335 19 335 3.66e-48 169
MS.gene08225.t1 MTR_4g132280 29.318 440 278 15 11 435 45 466 9.33e-48 172
MS.gene08225.t1 MTR_8g085720 29.571 443 274 17 11 435 26 448 2.14e-47 171
MS.gene08225.t1 MTR_4g076500 33.231 325 198 11 8 327 75 385 2.58e-47 168
MS.gene08225.t1 MTR_3g083580 29.519 437 276 17 11 433 26 444 3.87e-47 170
MS.gene08225.t1 MTR_7g081370 32.615 325 188 11 9 329 26 323 9.13e-47 164
MS.gene08225.t1 MTR_4g084480 32.845 341 208 12 2 333 24 352 1.34e-46 166
MS.gene08225.t1 MTR_4g084480 32.845 341 208 12 2 333 24 352 1.43e-46 166
MS.gene08225.t1 MTR_4g084480 32.845 341 208 12 2 333 17 345 1.74e-46 166
MS.gene08225.t1 MTR_4g052970 31.373 357 221 15 10 355 28 371 2.65e-46 165
MS.gene08225.t1 MTR_5g078200 29.245 424 271 14 25 435 4 411 5.31e-46 169
MS.gene08225.t1 MTR_2g086530 31.563 339 210 13 6 335 24 349 8.34e-45 161
MS.gene08225.t1 MTR_2g086530 31.563 339 210 13 6 335 3 328 9.72e-45 160
MS.gene08225.t1 MTR_3g116510 34.650 329 193 15 120 435 1 320 3.60e-43 155
MS.gene08225.t1 MTR_1g022160 32.036 334 207 11 11 334 24 347 4.54e-43 157
MS.gene08225.t1 MTR_2g019345 32.812 320 194 11 12 322 25 332 2.89e-42 154
MS.gene08225.t1 MTR_8g102340 30.682 352 223 12 78 419 62 402 4.10e-42 156
MS.gene08225.t1 MTR_7g053500 27.088 443 293 18 12 435 27 458 2.69e-41 153
MS.gene08225.t1 MTR_8g012400 29.679 374 235 16 78 435 17 378 7.11e-37 139
MS.gene08225.t1 MTR_7g053500 28.954 373 236 17 81 435 20 381 4.67e-34 132
MS.gene08225.t1 MTR_5g081720 27.778 324 212 13 28 342 54 364 1.01e-25 108
MS.gene08225.t1 MTR_4g036680 31.132 106 68 3 226 330 75 176 1.83e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08225.t1 AT1G64760 75.682 440 106 1 1 440 15 453 0.0 688
MS.gene08225.t1 AT1G64760 75.682 440 106 1 1 440 15 453 0.0 688
MS.gene08225.t1 AT2G19440 76.212 433 102 1 8 440 18 449 0.0 686
MS.gene08225.t1 AT2G19440 76.212 433 102 1 8 440 18 449 0.0 686
MS.gene08225.t1 AT3G04010 68.080 448 141 2 9 455 31 477 0.0 629
MS.gene08225.t1 AT5G18220 67.974 459 135 5 9 462 28 479 0.0 625
MS.gene08225.t1 AT3G24330 54.167 456 206 3 11 464 39 493 1.65e-176 504
MS.gene08225.t1 AT5G64790 57.011 435 184 2 10 443 27 459 4.06e-174 498
MS.gene08225.t1 AT4G31140 54.741 464 197 6 9 464 25 483 2.19e-172 493
MS.gene08225.t1 AT5G58090 53.493 458 206 5 5 458 16 470 1.04e-168 483
MS.gene08225.t1 AT5G20870 52.381 441 195 6 9 439 28 463 1.22e-149 436
MS.gene08225.t1 AT4G17180 49.091 440 211 5 9 446 23 451 1.63e-147 429
MS.gene08225.t1 AT5G58480 44.170 446 237 7 9 451 24 460 7.01e-124 369
MS.gene08225.t1 AT5G55180 33.182 440 269 15 11 435 27 456 1.42e-66 221
MS.gene08225.t1 AT5G55180 33.803 426 257 15 11 421 27 442 2.45e-66 220
MS.gene08225.t1 AT2G16230 33.790 438 264 16 11 435 25 449 3.47e-65 218
MS.gene08225.t1 AT2G05790 33.333 465 255 21 8 435 22 468 3.87e-64 214
MS.gene08225.t1 AT3G55430 34.318 440 259 17 9 435 27 449 9.28e-63 210
MS.gene08225.t1 AT4G26830 32.045 440 271 16 11 435 25 451 6.24e-59 201
MS.gene08225.t1 AT2G39640 33.181 437 256 17 9 433 28 440 7.14e-59 202
MS.gene08225.t1 AT4G26830 32.273 440 270 17 11 435 29 455 7.59e-59 200
MS.gene08225.t1 AT2G01630 31.111 450 274 17 11 446 23 450 2.85e-58 200
MS.gene08225.t1 AT3G13560 33.333 444 266 15 5 435 20 446 2.65e-57 197
MS.gene08225.t1 AT3G13560 33.333 444 266 15 5 435 20 446 2.65e-57 197
MS.gene08225.t1 AT3G13560 33.333 444 266 15 5 435 20 446 2.65e-57 197
MS.gene08225.t1 AT3G13560 33.333 444 266 15 5 435 20 446 2.65e-57 197
MS.gene08225.t1 AT3G13560 33.333 444 266 15 5 435 20 446 2.65e-57 197
MS.gene08225.t1 AT2G01630 30.716 433 269 16 11 429 23 438 6.58e-57 195
MS.gene08225.t1 AT4G34480 33.030 439 263 17 11 435 26 447 1.55e-56 195
MS.gene08225.t1 AT3G07320 32.200 441 271 18 12 435 26 455 1.68e-56 194
MS.gene08225.t1 AT1G66250 30.543 442 269 15 11 435 32 452 5.07e-56 194
MS.gene08225.t1 AT4G14080 31.250 448 275 20 8 435 39 473 2.61e-54 189
MS.gene08225.t1 AT1G11820 30.023 443 271 16 11 435 44 465 1.07e-53 188
MS.gene08225.t1 AT4G16260 32.722 327 206 7 9 333 22 336 1.48e-53 183
MS.gene08225.t1 AT5G24318 29.241 448 274 18 10 435 29 455 2.60e-53 186
MS.gene08225.t1 AT5G56590 29.075 454 281 17 5 436 20 454 3.09e-53 186
MS.gene08225.t1 AT3G23770 31.707 451 272 21 8 435 34 471 4.44e-53 186
MS.gene08225.t1 AT5G24318 29.452 438 266 18 10 425 29 445 8.29e-53 184
MS.gene08225.t1 AT1G11820 29.977 437 267 16 11 429 44 459 1.46e-52 184
MS.gene08225.t1 AT4G16260 33.228 316 196 8 21 333 4 307 2.26e-52 179
MS.gene08225.t1 AT3G57270 33.951 324 200 9 11 331 27 339 4.04e-51 177
MS.gene08225.t1 AT2G26600 31.714 350 215 12 12 348 35 373 1.48e-49 174
MS.gene08225.t1 AT5G42720 35.514 321 189 11 11 322 26 337 2.50e-49 174
MS.gene08225.t1 AT3G57260 33.233 331 201 9 4 331 25 338 2.89e-49 172
MS.gene08225.t1 AT3G57260 33.233 331 201 9 4 331 41 354 3.79e-49 172
MS.gene08225.t1 AT1G30080 32.121 330 204 10 2 322 27 345 1.25e-48 171
MS.gene08225.t1 AT1G30080 32.121 330 204 10 2 322 27 345 2.48e-48 171
MS.gene08225.t1 AT3G15800 33.333 333 200 12 12 333 44 365 2.66e-48 171
MS.gene08225.t1 AT4G34480 36.533 323 182 14 11 322 26 336 2.17e-47 167
MS.gene08225.t1 AT5G42100 34.821 336 196 13 8 331 24 348 2.86e-47 169
MS.gene08225.t1 AT5G42100 34.821 336 196 13 8 331 24 348 4.89e-47 168
MS.gene08225.t1 AT1G32860 33.538 325 192 12 11 323 29 341 8.44e-47 167
MS.gene08225.t1 AT2G01630 31.138 334 209 11 11 333 23 346 5.62e-46 164
MS.gene08225.t1 AT5G24318 30.409 342 208 13 1 327 16 342 1.13e-44 160
MS.gene08225.t1 AT3G57240 31.776 321 196 11 11 327 35 336 3.14e-44 158
MS.gene08225.t1 AT4G29360 26.452 465 296 15 8 437 34 487 4.17e-44 162
MS.gene08225.t1 AT4G29360 26.452 465 296 15 8 437 24 477 4.41e-44 162
MS.gene08225.t1 AT4G18340 30.931 333 208 12 10 332 33 353 1.92e-42 155
MS.gene08225.t1 AT4G18340 30.723 332 208 12 2 323 25 344 2.76e-42 154
MS.gene08225.t1 AT1G11820 30.030 333 214 10 11 333 44 367 3.14e-42 154
MS.gene08225.t1 AT3G61810 28.869 336 207 12 11 332 55 372 6.11e-40 147
MS.gene08225.t1 AT2G27500 29.377 337 217 11 9 335 27 352 8.22e-40 147
MS.gene08225.t1 AT2G27500 28.933 356 230 13 9 353 27 370 1.56e-39 147
MS.gene08225.t1 AT4G34480 32.665 349 207 15 100 435 3 336 4.17e-39 146
MS.gene08225.t1 AT3G46570 30.303 330 207 14 8 327 24 340 2.47e-37 140
MS.gene08225.t1 AT2G26600 30.714 280 171 11 81 348 11 279 2.01e-34 130
MS.gene08225.t1 AT1G77780 29.412 323 208 9 12 329 25 332 2.31e-34 132
MS.gene08225.t1 AT5G20390 28.829 333 215 11 4 329 26 343 2.37e-33 129
MS.gene08225.t1 AT5G24318 29.341 334 197 15 123 435 1 316 4.99e-33 127
MS.gene08225.t1 AT5G20340 27.640 322 212 10 4 319 36 342 6.83e-31 122
MS.gene08225.t1 AT5G20330 27.019 322 214 10 4 319 27 333 2.08e-30 121
MS.gene08225.t1 AT1G33220 27.922 308 204 9 4 305 27 322 3.30e-30 120
MS.gene08225.t1 AT3G55780 26.000 350 229 12 11 342 31 368 7.83e-30 120
MS.gene08225.t1 AT1G77790 28.939 311 198 11 25 327 44 339 1.39e-29 119
MS.gene08225.t1 AT2G27500 28.618 304 194 13 61 353 1 292 4.30e-29 116
MS.gene08225.t1 AT5G20560 27.044 318 212 10 8 319 31 334 2.41e-28 115

Find 114 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATATAGACATCTGCGTTTA+AGG 0.135341 7.3:+23150770 None:intergenic
CATCAAAGACGTTGGTATAT+TGG 0.201622 7.3:+23150538 None:intergenic
ATTATAAACGTGCTCAATAT+TGG 0.203608 7.3:-23152414 MS.gene08225:CDS
AACATCTTCACAACAGTTTC+TGG 0.211606 7.3:+23152562 None:intergenic
TGGATTCCATTGTCAGTTAT+TGG 0.212683 7.3:+23150558 None:intergenic
ATTTCAGCAAGTTGATTATT+AGG 0.225555 7.3:+23152442 None:intergenic
CCTACTTCTCCTACCAAAAT+TGG 0.271582 7.3:+23150462 None:intergenic
CGGAAGGGCTTGGTGTGAAC+TGG 0.274756 7.3:-23152612 MS.gene08225:CDS
TGCAACAACCTTGATGCTAA+TGG 0.283164 7.3:-23150039 MS.gene08225:CDS
TTTCGGCGACACGCCAATTT+TGG 0.288643 7.3:-23150475 MS.gene08225:CDS
AAGTTTCCGATGGATCTTTC+GGG 0.294935 7.3:-23150225 MS.gene08225:CDS
GCTCTCAAATCGATCGGTTT+CGG 0.295941 7.3:-23150492 MS.gene08225:CDS
TAAGGGTACTGTTGCCTTTA+CGG 0.296654 7.3:+23150788 None:intergenic
ATTAACTTTGCTTGCACCTT+TGG 0.307246 7.3:-23150093 MS.gene08225:CDS
TTTATCGAAGTTTATCTGTT+TGG 0.328813 7.3:-23150330 MS.gene08225:CDS
GGGTAAATGTTGACAGTAAA+TGG 0.333092 7.3:+23150648 None:intergenic
TTCCATCTGCGGGAATATTT+CGG 0.334659 7.3:-23150728 MS.gene08225:CDS
TCTTATGGCATTGCCGGAAT+TGG 0.344742 7.3:+23150413 None:intergenic
TTATAAACGTGCTCAATATT+GGG 0.352564 7.3:-23152413 MS.gene08225:CDS
GTGAAGATGTTGAAGGATAA+TGG 0.352574 7.3:-23152550 MS.gene08225:CDS
GTTACTCGTTATAACTTTGA+TGG 0.354819 7.3:-23152379 MS.gene08225:CDS
GTAGAATCTTATGGCATTGC+CGG 0.361755 7.3:+23150407 None:intergenic
CAGATAAACTTCGATAAATC+CGG 0.367845 7.3:+23150335 None:intergenic
GGTGACAAGAATGCCAATTC+CGG 0.398140 7.3:-23150426 MS.gene08225:CDS
GAAGTTTCCGATGGATCTTT+CGG 0.401121 7.3:-23150226 MS.gene08225:CDS
TTGCTGAGATCTTTAGCATC+AGG 0.401772 7.3:+23150129 None:intergenic
CGATAAATCCGGGGCGGAGA+GGG 0.404972 7.3:+23150346 None:intergenic
TCGAAGAAGGCGAAGTTGAA+TGG 0.408365 7.3:+23150594 None:intergenic
AGTCATGAGACCATTGATAT+CGG 0.412963 7.3:+23150704 None:intergenic
GACGATCCTGATTCTGCATC+TGG 0.421771 7.3:+23149986 None:intergenic
TCAACTTCGCCTTCTTCGAC+GGG 0.422981 7.3:-23150590 MS.gene08225:CDS
TGAAGATGTTGAAGGATAAT+GGG 0.423140 7.3:-23152549 MS.gene08225:CDS
GAGGCCATTGTAGAATCTTA+TGG 0.428508 7.3:+23150398 None:intergenic
TTCAACTTCGCCTTCTTCGA+CGG 0.430662 7.3:-23150591 MS.gene08225:CDS
TGTTGTTATGCGGAAGGGCT+TGG 0.438672 7.3:-23152622 MS.gene08225:CDS
ACCAAGACCAGCTTCATTGA+TGG 0.438757 7.3:+23150815 None:intergenic
TTTATCTACCCCGTCGAAGA+AGG 0.451188 7.3:+23150581 None:intergenic
TCCGCAGATATGTAGCGGTT+GGG 0.453302 7.3:-23150914 MS.gene08225:intron
TACCCCTTCTTAAGTCTCTA+CGG 0.454310 7.3:-23150630 MS.gene08225:CDS
ATGCTGAAGAAGGAACTATC+TGG 0.458076 7.3:+23149890 None:intergenic
GTACTACCAGATGCAGAATC+AGG 0.461536 7.3:-23149992 MS.gene08225:CDS
TGATACTACAATGACTGCTT+TGG 0.463045 7.3:-23152497 MS.gene08225:CDS
CTTCAAAACAAACTTCTAGT+AGG 0.464131 7.3:-23150198 MS.gene08225:CDS
GCATTCTTGTCACCTTCTGT+TGG 0.465889 7.3:+23150435 None:intergenic
AAGCATTTCCATTAGCATCA+AGG 0.472386 7.3:+23150031 None:intergenic
CGAAGAAGGCGAAGTTGAAT+GGG 0.474909 7.3:+23150595 None:intergenic
TTCCGCAGATATGTAGCGGT+TGG 0.484860 7.3:-23150915 MS.gene08225:intron
ACCCCTTCTTAAGTCTCTAC+GGG 0.484918 7.3:-23150629 MS.gene08225:CDS
GGAAGGGCTTGGTGTGAACT+GGG 0.486132 7.3:-23152611 MS.gene08225:CDS
CAGTGTCTCTCAAAGTTACC+TGG 0.486453 7.3:+23150276 None:intergenic
GATTGTACTGCACTTGGGTA+TGG 0.490793 7.3:-23150069 MS.gene08225:CDS
TGATGCAAATTTCGATACAT+TGG 0.491804 7.3:-23150520 MS.gene08225:CDS
AAACAACAAAGCAGCAAATG+AGG 0.493412 7.3:+23149855 None:intergenic
TGGGGAATATTTCGATATGA+TGG 0.495745 7.3:-23150255 MS.gene08225:CDS
TGTTCGCAGCGAGTCTAGAA+AGG 0.496041 7.3:+23150376 None:intergenic
GCCATCAATGAAGCTGGTCT+TGG 0.496624 7.3:-23150816 MS.gene08225:CDS
AACGTGCTCAATATTGGGTT+AGG 0.498245 7.3:-23152408 MS.gene08225:CDS
GTTGAAATGTTGTCTGTAGT+AGG 0.500081 7.3:+23149934 None:intergenic
ATGACTGCTTTGGCTGGTAG+TGG 0.501400 7.3:-23152487 MS.gene08225:CDS
TGGTAGTGGAATTGAAGTTA+TGG 0.502034 7.3:-23152473 MS.gene08225:CDS
TTTGGAGATTGTACTGCACT+TGG 0.515034 7.3:-23150075 MS.gene08225:CDS
GTCATGAGACCATTGATATC+GGG 0.521177 7.3:+23150705 None:intergenic
GTTTCTGCTCTCAAATCGAT+CGG 0.522943 7.3:-23150498 MS.gene08225:CDS
CAAAATGCCATCAATGAAGC+TGG 0.527897 7.3:-23150822 MS.gene08225:CDS
GGCCGAAATATTCCCGCAGA+TGG 0.529991 7.3:+23150726 None:intergenic
ATCAAAGAAGATGCTGAAGA+AGG 0.530104 7.3:+23149880 None:intergenic
AGATAAACTTCGATAAATCC+GGG 0.532252 7.3:+23150336 None:intergenic
TGCTGAGATCTTTAGCATCA+GGG 0.538134 7.3:+23150130 None:intergenic
CAAACTTCTAGTAGGTGCAC+AGG 0.538210 7.3:-23150190 MS.gene08225:CDS
ATATAGACATCTGCGTTTAA+GGG 0.538392 7.3:+23150771 None:intergenic
CAGGTAACTTTGAGAGACAC+TGG 0.539845 7.3:-23150275 MS.gene08225:CDS
GACAATCAAGTTCCATCTGC+GGG 0.543282 7.3:-23150738 MS.gene08225:CDS
TCGATAAATCCGGGGCGGAG+AGG 0.551129 7.3:+23150345 None:intergenic
GACGCGAAGAGCATTGCTCC+AGG 0.552654 7.3:-23150294 MS.gene08225:CDS
ACTACAATGACTGCTTTGGC+TGG 0.558501 7.3:-23152493 MS.gene08225:CDS
ATATTTCGGCCCGATATCAA+TGG 0.563489 7.3:-23150714 MS.gene08225:CDS
TTGGAGATTGTACTGCACTT+GGG 0.571116 7.3:-23150074 MS.gene08225:CDS
AACTGTTGTGAAGATGTTGA+AGG 0.573217 7.3:-23152557 MS.gene08225:CDS
GATAAACCAATAACTGACAA+TGG 0.575139 7.3:-23150564 MS.gene08225:CDS
TCTAGACTCGCTGCGAACAA+AGG 0.575607 7.3:-23150372 MS.gene08225:CDS
AAAGGAACCCCTCTCCGCCC+CGG 0.576993 7.3:-23150354 MS.gene08225:CDS
GAAAGATCCATCGGAAACTT+CGG 0.579080 7.3:+23150228 None:intergenic
GAAGTAGGATGGCCAACAGA+AGG 0.581640 7.3:-23150447 MS.gene08225:CDS
TGGTCTTGGTGACACCGTAA+AGG 0.581918 7.3:-23150802 MS.gene08225:CDS
AATGTTCTGCAGTGCAGGAA+AGG 0.584113 7.3:+23150845 None:intergenic
CAATGGTCTCATGACTCAGA+TGG 0.589085 7.3:-23150697 MS.gene08225:CDS
GAAATTTGCATCAAAGACGT+TGG 0.589177 7.3:+23150530 None:intergenic
AGGTAACTTTGAGAGACACT+GGG 0.591795 7.3:-23150274 MS.gene08225:CDS
GATAAATCCGGGGCGGAGAG+GGG 0.601391 7.3:+23150347 None:intergenic
GTTCCCGTAGAGACTTAAGA+AGG 0.603438 7.3:+23150626 None:intergenic
AACAACAAAGCAGCAAATGA+GGG 0.604408 7.3:+23149856 None:intergenic
AGATGTCTATATGTCTCCGC+AGG 0.611737 7.3:-23150760 MS.gene08225:CDS
ACTCGTTATAACTTTGATGG+CGG 0.617236 7.3:-23152376 MS.gene08225:CDS
GGGTTAAACATGCACCACAT+TGG 0.618943 7.3:+23150150 None:intergenic
TGGATGATAAAATTGCAAGT+AGG 0.623501 7.3:+23149910 None:intergenic
TTCCCGTAGAGACTTAAGAA+GGG 0.632332 7.3:+23150627 None:intergenic
GTACAATTTCTCAACAAGAA+CGG 0.637978 7.3:-23150675 MS.gene08225:CDS
ATCTTCACAACAGTTTCTGG+TGG 0.639053 7.3:+23152565 None:intergenic
AGTTGACCCGAAAGATCCAT+CGG 0.649595 7.3:+23150219 None:intergenic
TCCCGTAGAGACTTAAGAAG+GGG 0.652260 7.3:+23150628 None:intergenic
GGACAATCAAGTTCCATCTG+CGG 0.652978 7.3:-23150739 MS.gene08225:CDS
GAAGGGCTTGGTGTGAACTG+GGG 0.653748 7.3:-23152610 MS.gene08225:CDS
AAGTGCAGTACAATCTCCAA+AGG 0.655496 7.3:+23150077 None:intergenic
TGGACAACCGAAGTTTCCGA+TGG 0.662375 7.3:-23150235 MS.gene08225:CDS
AAACTTCTAGTAGGTGCACA+GGG 0.662815 7.3:-23150189 MS.gene08225:CDS
TCTGTTTGGACTCATCGATG+AGG 0.680701 7.3:-23150316 MS.gene08225:CDS
TCGCAGCGAGTCTAGAAAGG+AGG 0.683390 7.3:+23150379 None:intergenic
AATGCCATAAGATTCTACAA+TGG 0.690889 7.3:-23150402 MS.gene08225:CDS
TGAAATATCTTGATCCAATG+TGG 0.694528 7.3:-23150164 MS.gene08225:CDS
CAACTTCGCCTTCTTCGACG+GGG 0.696178 7.3:-23150589 MS.gene08225:CDS
GATGGAACTTGATTGTCCTG+CGG 0.701807 7.3:+23150744 None:intergenic
GATAAACTTCGATAAATCCG+GGG 0.709966 7.3:+23150337 None:intergenic
AAACTTCGATAAATCCGGGG+CGG 0.736359 7.3:+23150340 None:intergenic
GGTAACTTTGAGAGACACTG+GGG 0.786507 7.3:-23150273 MS.gene08225:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTTTTATTTTTGTCA+GGG - chr7.3:23150422-23150441 MS.gene08225:CDS 10.0%
!!! TTTTTATTTTTATTTTTGTC+AGG - chr7.3:23150421-23150440 MS.gene08225:CDS 10.0%
!! AATAAAAGAATCTAATTCTG+AGG + chr7.3:23150631-23150650 None:intergenic 20.0%
!! AATCATTATTATGAACACAA+AGG + chr7.3:23150479-23150498 None:intergenic 20.0%
!! ATAAAAGAATCTAATTCTGA+GGG + chr7.3:23150630-23150649 None:intergenic 20.0%
!! ATCATTATTATGAACACAAA+GGG + chr7.3:23150478-23150497 None:intergenic 20.0%
!! TTATTGTAAGCTTTCAAAAA+TGG + chr7.3:23151577-23151596 None:intergenic 20.0%
!!! GATTTTATTGAACATTTTGA+AGG - chr7.3:23151171-23151190 MS.gene08225:intron 20.0%
!!! TCAGAATTAGATTCTTTTAT+TGG - chr7.3:23150630-23150649 MS.gene08225:CDS 20.0%
!!! TTTGTTAGATTTTTGATTCA+TGG - chr7.3:23150387-23150406 MS.gene08225:CDS 20.0%
! AGAACAAATACATTATACCT+CGG + chr7.3:23150698-23150717 None:intergenic 25.0%
! ATGTGCATATATCATATTCT+TGG - chr7.3:23150796-23150815 MS.gene08225:CDS 25.0%
! ATTATAAACGTGCTCAATAT+TGG - chr7.3:23150045-23150064 MS.gene08225:CDS 25.0%
! ATTCATCAAAGCTTAAAATG+TGG + chr7.3:23151222-23151241 None:intergenic 25.0%
! CATATATCATATTCTTGGAA+TGG - chr7.3:23150801-23150820 MS.gene08225:CDS 25.0%
! GCACAAATTCACATTAAAAT+CGG + chr7.3:23151509-23151528 None:intergenic 25.0%
! TGGTGTTTAATAATAAGAAG+CGG + chr7.3:23151202-23151221 None:intergenic 25.0%
! TTATAAACGTGCTCAATATT+GGG - chr7.3:23150046-23150065 MS.gene08225:CDS 25.0%
! TTGACAACACTAGATTTAAT+GGG - chr7.3:23151149-23151168 MS.gene08225:intron 25.0%
! TTTATCGAAGTTTATCTGTT+TGG - chr7.3:23152129-23152148 MS.gene08225:intron 25.0%
! TTTGACAACACTAGATTTAA+TGG - chr7.3:23151148-23151167 MS.gene08225:intron 25.0%
!! GTCTGTTCAAACAATTTTAA+AGG - chr7.3:23150995-23151014 MS.gene08225:intron 25.0%
!! TATTTTTCACAATCAGCAAT+TGG + chr7.3:23151083-23151102 None:intergenic 25.0%
!! TTAGATTCTTTTATTGGTGT+TGG - chr7.3:23150636-23150655 MS.gene08225:CDS 25.0%
!! TTTTCCTTCTGTATTATAGT+TGG - chr7.3:23150962-23150981 MS.gene08225:intron 25.0%
!!! AATTTTTCGAGATTTTGTGT+TGG - chr7.3:23150225-23150244 MS.gene08225:CDS 25.0%
!!! AGTTGTTCTTGTTTTAATCA+AGG - chr7.3:23150582-23150601 MS.gene08225:CDS 25.0%
!!! ATTTCAGCAAGTTGATTATT+AGG + chr7.3:23150020-23150039 None:intergenic 25.0%
!!! CAATTTTAAAGGAGTTAGAT+TGG - chr7.3:23151006-23151025 MS.gene08225:intron 25.0%
!!! TAGATTTTTGATTCATGGTT+AGG - chr7.3:23150392-23150411 MS.gene08225:CDS 25.0%
!!! TGATTATGCATTTTGATTGA+TGG - chr7.3:23150500-23150519 MS.gene08225:CDS 25.0%
AAATCTAGTGTTGTCAAAAG+CGG + chr7.3:23151146-23151165 None:intergenic 30.0%
AATGCCATAAGATTCTACAA+TGG - chr7.3:23152057-23152076 MS.gene08225:intron 30.0%
AGATAAACTTCGATAAATCC+GGG + chr7.3:23152126-23152145 None:intergenic 30.0%
ATATAGACATCTGCGTTTAA+GGG + chr7.3:23151691-23151710 None:intergenic 30.0%
CAGATAAACTTCGATAAATC+CGG + chr7.3:23152127-23152146 None:intergenic 30.0%
CATTAAAATCGGTCGAAATT+CGG + chr7.3:23151498-23151517 None:intergenic 30.0%
CTTCAAAACAAACTTCTAGT+AGG - chr7.3:23152261-23152280 MS.gene08225:intron 30.0%
GATAAACCAATAACTGACAA+TGG - chr7.3:23151895-23151914 MS.gene08225:intron 30.0%
GTACAATTTCTCAACAAGAA+CGG - chr7.3:23151784-23151803 MS.gene08225:intron 30.0%
GTTACTCGTTATAACTTTGA+TGG - chr7.3:23150080-23150099 MS.gene08225:CDS 30.0%
TATCATATTCTTGGAATGGA+AGG - chr7.3:23150805-23150824 MS.gene08225:CDS 30.0%
TCAACCAACTATAATACAGA+AGG + chr7.3:23150969-23150988 None:intergenic 30.0%
TGAAATATCTTGATCCAATG+TGG - chr7.3:23152295-23152314 MS.gene08225:intron 30.0%
TGAAGATGTTGAAGGATAAT+GGG - chr7.3:23149910-23149929 MS.gene08225:CDS 30.0%
TGATGCAAATTTCGATACAT+TGG - chr7.3:23151939-23151958 MS.gene08225:intron 30.0%
TGATTGAGAGTTCATATAAC+AGG - chr7.3:23150878-23150897 MS.gene08225:CDS 30.0%
TGGATGATAAAATTGCAAGT+AGG + chr7.3:23152552-23152571 None:intergenic 30.0%
! AATCATTTTACCATTCTAGG+AGG - chr7.3:23150721-23150740 MS.gene08225:CDS 30.0%
! ATCAAGGAAGCTTTATGTAT+TGG - chr7.3:23150598-23150617 MS.gene08225:CDS 30.0%
! GAGAATCATTTTACCATTCT+AGG - chr7.3:23150718-23150737 MS.gene08225:CDS 30.0%
! GCTTGTAGAGATTTTATTGA+TGG - chr7.3:23151030-23151049 MS.gene08225:intron 30.0%
! TAACAGGAAATTTTGCATCA+AGG - chr7.3:23150894-23150913 MS.gene08225:CDS 30.0%
! TAGCGAATAATTTGTAATGC+TGG - chr7.3:23151327-23151346 MS.gene08225:intron 30.0%
! TCAAGGAAGCTTTATGTATT+GGG - chr7.3:23150599-23150618 MS.gene08225:CDS 30.0%
!! AAAGATGGCATTGATGAATT+TGG + chr7.3:23151427-23151446 None:intergenic 30.0%
!! AAGATGGCATTGATGAATTT+GGG + chr7.3:23151426-23151445 None:intergenic 30.0%
!!! TGCTTTTTCTGTGATTCTAT+TGG - chr7.3:23152629-23152648 MS.gene08225:CDS 30.0%
!!! TTTGATTGATGGTGAGTTTT+GGG - chr7.3:23150511-23150530 MS.gene08225:CDS 30.0%
!!! TTTTGATTGATGGTGAGTTT+TGG - chr7.3:23150510-23150529 MS.gene08225:CDS 30.0%
AAACAACAAAGCAGCAAATG+AGG + chr7.3:23152607-23152626 None:intergenic 35.0%
AACAACAAAGCAGCAAATGA+GGG + chr7.3:23152606-23152625 None:intergenic 35.0%
AACATCTTCACAACAGTTTC+TGG + chr7.3:23149900-23149919 None:intergenic 35.0%
AACTGTTGTGAAGATGTTGA+AGG - chr7.3:23149902-23149921 MS.gene08225:CDS 35.0%
AAGCATTTCCATTAGCATCA+AGG + chr7.3:23152431-23152450 None:intergenic 35.0%
ACTCGTTATAACTTTGATGG+CGG - chr7.3:23150083-23150102 MS.gene08225:CDS 35.0%
AGTCATGAGACCATTGATAT+CGG + chr7.3:23151758-23151777 None:intergenic 35.0%
ATCAAAGAAGATGCTGAAGA+AGG + chr7.3:23152582-23152601 None:intergenic 35.0%
ATTAACTTTGCTTGCACCTT+TGG - chr7.3:23152366-23152385 MS.gene08225:CDS 35.0%
CACAGAAAAAAGTTGCTACA+AGG + chr7.3:23150675-23150694 None:intergenic 35.0%
CATATAGACATCTGCGTTTA+AGG + chr7.3:23151692-23151711 None:intergenic 35.0%
CATCAAAGACGTTGGTATAT+TGG + chr7.3:23151924-23151943 None:intergenic 35.0%
CCAATCCAATGACAAAAACA+AGG + chr7.3:23151371-23151390 None:intergenic 35.0%
GAAATTTGCATCAAAGACGT+TGG + chr7.3:23151932-23151951 None:intergenic 35.0%
GATAAACTTCGATAAATCCG+GGG + chr7.3:23152125-23152144 None:intergenic 35.0%
GGGTAAATGTTGACAGTAAA+TGG + chr7.3:23151814-23151833 None:intergenic 35.0%
GTATAATGTGCTTGCTTCAT+TGG - chr7.3:23150270-23150289 MS.gene08225:CDS 35.0%
GTGAAGATGTTGAAGGATAA+TGG - chr7.3:23149909-23149928 MS.gene08225:CDS 35.0%
GTTGAAATGTTGTCTGTAGT+AGG + chr7.3:23152528-23152547 None:intergenic 35.0%
TACATATCTGCGGAAAAAGA+TGG + chr7.3:23151539-23151558 None:intergenic 35.0%
TCATCAATGCCATCTTTAGT+TGG - chr7.3:23151430-23151449 MS.gene08225:intron 35.0%
TGAAGCAAGCACATTATACA+AGG + chr7.3:23150269-23150288 None:intergenic 35.0%
TGATACTACAATGACTGCTT+TGG - chr7.3:23149962-23149981 MS.gene08225:CDS 35.0%
TGGATTCCATTGTCAGTTAT+TGG + chr7.3:23151904-23151923 None:intergenic 35.0%
TGGGGAATATTTCGATATGA+TGG - chr7.3:23152204-23152223 MS.gene08225:intron 35.0%
TTGGTCAAACACTAAAATGG+TGG - chr7.3:23151387-23151406 MS.gene08225:intron 35.0%
!! AGATGGCATTGATGAATTTG+GGG + chr7.3:23151425-23151444 None:intergenic 35.0%
!! GGATTGGTCAAACACTAAAA+TGG - chr7.3:23151384-23151403 MS.gene08225:intron 35.0%
!! TGGTAGTGGAATTGAAGTTA+TGG - chr7.3:23149986-23150005 MS.gene08225:CDS 35.0%
!!! CAGTTTTAGGGGAAAAGAAA+TGG - chr7.3:23150750-23150769 MS.gene08225:CDS 35.0%
!!! CCTTGTTTTTGTCATTGGAT+TGG - chr7.3:23151368-23151387 MS.gene08225:intron 35.0%
!!! GGGTTGTTTTTGTTGTTATG+CGG - chr7.3:23149827-23149846 MS.gene08225:CDS 35.0%
!!! GTTTTTGTTGTTATGCGGAA+GGG - chr7.3:23149832-23149851 MS.gene08225:CDS 35.0%
!!! TGTTTTTGTTGTTATGCGGA+AGG - chr7.3:23149831-23149850 MS.gene08225:CDS 35.0%
AAACTTCTAGTAGGTGCACA+GGG - chr7.3:23152270-23152289 MS.gene08225:intron 40.0%
AACGTGCTCAATATTGGGTT+AGG - chr7.3:23150051-23150070 MS.gene08225:CDS 40.0%
AAGTGCAGTACAATCTCCAA+AGG + chr7.3:23152385-23152404 None:intergenic 40.0%
AAGTTTCCGATGGATCTTTC+GGG - chr7.3:23152234-23152253 MS.gene08225:intron 40.0%
ATATTTCGGCCCGATATCAA+TGG - chr7.3:23151745-23151764 MS.gene08225:intron 40.0%
ATGCTGAAGAAGGAACTATC+TGG + chr7.3:23152572-23152591 None:intergenic 40.0%
CAAAATGCCATCAATGAAGC+TGG - chr7.3:23151637-23151656 MS.gene08225:intron 40.0%
CCTACTTCTCCTACCAAAAT+TGG + chr7.3:23152000-23152019 None:intergenic 40.0%
CTACTGCTTCCAACTAAAGA+TGG + chr7.3:23151442-23151461 None:intergenic 40.0%
GAAAGATCCATCGGAAACTT+CGG + chr7.3:23152234-23152253 None:intergenic 40.0%
GAAGTTTCCGATGGATCTTT+CGG - chr7.3:23152233-23152252 MS.gene08225:intron 40.0%
GAGGCCATTGTAGAATCTTA+TGG + chr7.3:23152064-23152083 None:intergenic 40.0%
GTAGAATCTTATGGCATTGC+CGG + chr7.3:23152055-23152074 None:intergenic 40.0%
GTCATGAGACCATTGATATC+GGG + chr7.3:23151757-23151776 None:intergenic 40.0%
GTTTCTGCTCTCAAATCGAT+CGG - chr7.3:23151961-23151980 MS.gene08225:intron 40.0%
TACCCCTTCTTAAGTCTCTA+CGG - chr7.3:23151829-23151848 MS.gene08225:intron 40.0%
TGCAACAACCTTGATGCTAA+TGG - chr7.3:23152420-23152439 MS.gene08225:CDS 40.0%
TGCTGAGATCTTTAGCATCA+GGG + chr7.3:23152332-23152351 None:intergenic 40.0%
TTCCATCTGCGGGAATATTT+CGG - chr7.3:23151731-23151750 MS.gene08225:intron 40.0%
TTCCCGTAGAGACTTAAGAA+GGG + chr7.3:23151835-23151854 None:intergenic 40.0%
TTGCTGAGATCTTTAGCATC+AGG + chr7.3:23152333-23152352 None:intergenic 40.0%
TTGGAGATTGTACTGCACTT+GGG - chr7.3:23152385-23152404 MS.gene08225:CDS 40.0%
TTTGGAGATTGTACTGCACT+TGG - chr7.3:23152384-23152403 MS.gene08225:CDS 40.0%
! AGGAGGTGATTGCAGTTTTA+GGG - chr7.3:23150738-23150757 MS.gene08225:CDS 40.0%
! AGGTAACTTTGAGAGACACT+GGG - chr7.3:23152185-23152204 MS.gene08225:intron 40.0%
! ATCTTCACAACAGTTTCTGG+TGG + chr7.3:23149897-23149916 None:intergenic 40.0%
! CGTCAAGCATGCAATTTTGA+AGG - chr7.3:23152492-23152511 MS.gene08225:CDS 40.0%
! CTTTTTCCGCAGATATGTAG+CGG - chr7.3:23151540-23151559 MS.gene08225:intron 40.0%
! TAGGAGGTGATTGCAGTTTT+AGG - chr7.3:23150737-23150756 MS.gene08225:CDS 40.0%
! TTTTGAATGTTCTGCAGTGC+AGG + chr7.3:23151622-23151641 None:intergenic 40.0%
! TTTTGCATGTGCGCGTATAT+GGG - chr7.3:23150840-23150859 MS.gene08225:CDS 40.0%
! TTTTGGTAGGAGAAGTAGGA+TGG - chr7.3:23152001-23152020 MS.gene08225:intron 40.0%
! TTTTTGCATGTGCGCGTATA+TGG - chr7.3:23150839-23150858 MS.gene08225:CDS 40.0%
!! CCAATTTTGGTAGGAGAAGT+AGG - chr7.3:23151997-23152016 MS.gene08225:intron 40.0%
!! TAAGGGTACTGTTGCCTTTA+CGG + chr7.3:23151674-23151693 None:intergenic 40.0%
!!! GTGAGTTTTGGGATCAAAGA+AGG - chr7.3:23150522-23150541 MS.gene08225:CDS 40.0%
!!! TGTCGCCTTGTTTTTGTCAT+TGG - chr7.3:23151363-23151382 MS.gene08225:intron 40.0%
AAACTTCGATAAATCCGGGG+CGG + chr7.3:23152122-23152141 None:intergenic 45.0%
AAATCGGTCGAAATTCGGCA+AGG + chr7.3:23151493-23151512 None:intergenic 45.0%
AATGTTCTGCAGTGCAGGAA+AGG + chr7.3:23151617-23151636 None:intergenic 45.0%
ACCAAGACCAGCTTCATTGA+TGG + chr7.3:23151647-23151666 None:intergenic 45.0%
ACCCCTTCTTAAGTCTCTAC+GGG - chr7.3:23151830-23151849 MS.gene08225:intron 45.0%
ACTACAATGACTGCTTTGGC+TGG - chr7.3:23149966-23149985 MS.gene08225:CDS 45.0%
ACTATGTTGTAGCCCTGCTA+TGG - chr7.3:23151120-23151139 MS.gene08225:intron 45.0%
ACTGCAATCACCTCCTAGAA+TGG + chr7.3:23150734-23150753 None:intergenic 45.0%
AGATGTCTATATGTCTCCGC+AGG - chr7.3:23151699-23151718 MS.gene08225:intron 45.0%
AGTTGACCCGAAAGATCCAT+CGG + chr7.3:23152243-23152262 None:intergenic 45.0%
ATAGCAGGGCTACAACATAG+TGG + chr7.3:23151121-23151140 None:intergenic 45.0%
CAAACTTCTAGTAGGTGCAC+AGG - chr7.3:23152269-23152288 MS.gene08225:intron 45.0%
CAATGGTCTCATGACTCAGA+TGG - chr7.3:23151762-23151781 MS.gene08225:intron 45.0%
CAGTGTCTCTCAAAGTTACC+TGG + chr7.3:23152186-23152205 None:intergenic 45.0%
CGAAGAAGGCGAAGTTGAAT+GGG + chr7.3:23151867-23151886 None:intergenic 45.0%
GACAATCAAGTTCCATCTGC+GGG - chr7.3:23151721-23151740 MS.gene08225:intron 45.0%
GATGGAACTTGATTGTCCTG+CGG + chr7.3:23151718-23151737 None:intergenic 45.0%
GATTGTACTGCACTTGGGTA+TGG - chr7.3:23152390-23152409 MS.gene08225:CDS 45.0%
GCATTCTTGTCACCTTCTGT+TGG + chr7.3:23152027-23152046 None:intergenic 45.0%
GGACAATCAAGTTCCATCTG+CGG - chr7.3:23151720-23151739 MS.gene08225:intron 45.0%
GGGTTAAACATGCACCACAT+TGG + chr7.3:23152312-23152331 None:intergenic 45.0%
GGTGACAAGAATGCCAATTC+CGG - chr7.3:23152033-23152052 MS.gene08225:intron 45.0%
GTACTACCAGATGCAGAATC+AGG - chr7.3:23152467-23152486 MS.gene08225:CDS 45.0%
GTTCCCGTAGAGACTTAAGA+AGG + chr7.3:23151836-23151855 None:intergenic 45.0%
TCCCGTAGAGACTTAAGAAG+GGG + chr7.3:23151834-23151853 None:intergenic 45.0%
TCGAAGAAGGCGAAGTTGAA+TGG + chr7.3:23151868-23151887 None:intergenic 45.0%
TCTGTTTGGACTCATCGATG+AGG - chr7.3:23152143-23152162 MS.gene08225:intron 45.0%
TCTTATGGCATTGCCGGAAT+TGG + chr7.3:23152049-23152068 None:intergenic 45.0%
TTCAACTTCGCCTTCTTCGA+CGG - chr7.3:23151868-23151887 MS.gene08225:intron 45.0%
TTTATCTACCCCGTCGAAGA+AGG + chr7.3:23151881-23151900 None:intergenic 45.0%
! CAGGTAACTTTGAGAGACAC+TGG - chr7.3:23152184-23152203 MS.gene08225:intron 45.0%
! GCTCTCAAATCGATCGGTTT+CGG - chr7.3:23151967-23151986 MS.gene08225:intron 45.0%
! GGTAACTTTGAGAGACACTG+GGG - chr7.3:23152186-23152205 MS.gene08225:intron 45.0%
!! GGAGGTGATTGCAGTTTTAG+GGG - chr7.3:23150739-23150758 MS.gene08225:CDS 45.0%
!!! AGCAACTTTTTTCTGTGCCG+AGG - chr7.3:23150678-23150697 MS.gene08225:CDS 45.0%
ATGACTGCTTTGGCTGGTAG+TGG - chr7.3:23149972-23149991 MS.gene08225:CDS 50.0%
GAAGTAGGATGGCCAACAGA+AGG - chr7.3:23152012-23152031 MS.gene08225:intron 50.0%
GACGATCCTGATTCTGCATC+TGG + chr7.3:23152476-23152495 None:intergenic 50.0%
TCAACTTCGCCTTCTTCGAC+GGG - chr7.3:23151869-23151888 MS.gene08225:intron 50.0%
TCCCAACCGCTACATATCTG+CGG + chr7.3:23151549-23151568 None:intergenic 50.0%
TCCGCAGATATGTAGCGGTT+GGG - chr7.3:23151545-23151564 MS.gene08225:intron 50.0%
TCTAGACTCGCTGCGAACAA+AGG - chr7.3:23152087-23152106 MS.gene08225:intron 50.0%
TGGACAACCGAAGTTTCCGA+TGG - chr7.3:23152224-23152243 MS.gene08225:intron 50.0%
TGTCAAAAGCGGCCATAGCA+GGG + chr7.3:23151135-23151154 None:intergenic 50.0%
TGTTCGCAGCGAGTCTAGAA+AGG + chr7.3:23152086-23152105 None:intergenic 50.0%
TGTTGTTATGCGGAAGGGCT+TGG - chr7.3:23149837-23149856 MS.gene08225:CDS 50.0%
TTCCGCAGATATGTAGCGGT+TGG - chr7.3:23151544-23151563 MS.gene08225:intron 50.0%
TTGTCAAAAGCGGCCATAGC+AGG + chr7.3:23151136-23151155 None:intergenic 50.0%
! GCCATCAATGAAGCTGGTCT+TGG - chr7.3:23151643-23151662 MS.gene08225:intron 50.0%
! TGGTCTTGGTGACACCGTAA+AGG - chr7.3:23151657-23151676 MS.gene08225:intron 50.0%
! TTTCGGCGACACGCCAATTT+TGG - chr7.3:23151984-23152003 MS.gene08225:intron 50.0%
CAACTTCGCCTTCTTCGACG+GGG - chr7.3:23151870-23151889 MS.gene08225:intron 55.0%
GGCCGAAATATTCCCGCAGA+TGG + chr7.3:23151736-23151755 None:intergenic 55.0%
TCGCAGCGAGTCTAGAAAGG+AGG + chr7.3:23152083-23152102 None:intergenic 55.0%
!! GAAGGGCTTGGTGTGAACTG+GGG - chr7.3:23149849-23149868 MS.gene08225:CDS 55.0%
!! GGAAGGGCTTGGTGTGAACT+GGG - chr7.3:23149848-23149867 MS.gene08225:CDS 55.0%
!! GGCGACACGCCAATTTTGGT+AGG - chr7.3:23151988-23152007 MS.gene08225:intron 55.0%
CGATAAATCCGGGGCGGAGA+GGG + chr7.3:23152116-23152135 None:intergenic 60.0%
GATAAATCCGGGGCGGAGAG+GGG + chr7.3:23152115-23152134 None:intergenic 60.0%
TCGATAAATCCGGGGCGGAG+AGG + chr7.3:23152117-23152136 None:intergenic 60.0%
!! CGGAAGGGCTTGGTGTGAAC+TGG - chr7.3:23149847-23149866 MS.gene08225:CDS 60.0%
!! GACGCGAAGAGCATTGCTCC+AGG - chr7.3:23152165-23152184 MS.gene08225:intron 60.0%
AAAGGAACCCCTCTCCGCCC+CGG - chr7.3:23152105-23152124 MS.gene08225:intron 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 23149825 23152656 23149825 ID=MS.gene08225
chr7.3 mRNA 23149825 23152656 23149825 ID=MS.gene08225.t1;Parent=MS.gene08225
chr7.3 exon 23152364 23152656 23152364 ID=MS.gene08225.t1.exon1;Parent=MS.gene08225.t1
chr7.3 CDS 23152364 23152656 23152364 ID=cds.MS.gene08225.t1;Parent=MS.gene08225.t1
chr7.3 exon 23149825 23150929 23149825 ID=MS.gene08225.t1.exon2;Parent=MS.gene08225.t1
chr7.3 CDS 23149825 23150929 23149825 ID=cds.MS.gene08225.t1;Parent=MS.gene08225.t1
Gene Sequence

>MS.gene08225

ATGGGTTGTTTTTGTTGTTATGCGGAAGGGCTTGGTGTGAACTGGGGTACACAAGCAACACACAAGTTACCACCAGAAACTGTTGTGAAGATGTTGAAGGATAATGGGATTCAAAAAGTTAAGCTTTTTGATGCAGATGATACTACAATGACTGCTTTGGCTGGTAGTGGAATTGAAGTTATGGTTGCTATTCCTAATAATCAACTTGCTGAAATGAATGATTATAAACGTGCTCAATATTGGGTTAGGAAGAATGTTACTCGTTATAACTTTGATGGCGGTGTTAAGATCAAGTGAGTTTTTTATTTTTATTTTTTATAATTAGTTAATTGATTTGATCTGTTGATGCTATTGATATTGATCTGCATTTGATGTTTAAGTGTTTGATTATTTGTATTGAAATTTTTCGAGATTTTGTGTTGGATTTTTATTACTTTTAATCCTTGTATAATGTGCTTGCTTCATTGGTTTAGTGTCAGTTAATTGATTTTTTTTTTTAATGTTATTATTGAGTATTGATCTGCATTTGATGTTTAAGTCATTGATTGTCTATGTTGATTTTTTTGTTAGATTTTTGATTCATGGTTAGGTCTATTTTTTTATTTTTATTTTTGTCAGGGTTTACTTTCATAAATGTTAAATTTTTTAAGCCCTTTGTGTTCATAATAATGATTATGATTATGCATTTTGATTGATGGTGAGTTTTGGGATCAAAGAAGGAAAAGTGTGAAAAGAATTTTTCAAAAGTGAGCTCTGAAGTTGTTCTTGTTTTAATCAAGGAAGCTTTATGTATTGGGTGATTCCCTCAGAATTAGATTCTTTTATTGGTGTTGGTGCTTGATTGATTCCTTGTAGCAACTTTTTTCTGTGCCGAGGTATAATGTATTTGTTCTGAGAATCATTTTACCATTCTAGGAGGTGATTGCAGTTTTAGGGGAAAAGAAATGGTATCAGTCTCTATCTGATTTTGTATGTGCATATATCATATTCTTGGAATGGAAGGATTTTTTTTTTTTTTTGCATGTGCGCGTATATGGGAGAAAAATAAGTAATTGATTGAGAGTTCATATAACAGGAAATTTTGCATCAAGGAATAACAATACAATTAAAATTTGTGATGCATTCTTGTCTCTTCAGTTTTCCTTCTGTATTATAGTTGGTTGATGAGAAGTCTGTTCAAACAATTTTAAAGGAGTTAGATTGGTGCTTGTAGAGATTTTATTGATGGTGTCTTTTCATAATTTCATCAGTGTTGCCAATTGCTGATTGTGAAAAATAGCAGTTTATTCAATTCCACTATGTTGTAGCCCTGCTATGGCCGCTTTTGACAACACTAGATTTAATGGGATTTTATTGAACATTTTGAAGGAAAAGCCGCTTCTTATTATTAAACACCACATTTTAAGCTTTGATGAATATATATTGAATATTGATTGTTTCAACTTCTCTTTAAATGCTAATTTTGTTAAGTGTTAATTCTTAAGCAAACTTGTAAATCATATTAGCGAATAATTTGTAATGCTGGTTGCGATGTTTATTGTCGCCTTGTTTTTGTCATTGGATTGGTCAAACACTAAAATGGTGGTATTGTAATGCTCCCCAAATTCATCAATGCCATCTTTAGTTGGAAGCAGTAGTACTATCAATGAAATGTATAGTTTATTTCCTTGCCGAATTTCGACCGATTTTAATGTGAATTTGTGCATTTTGTCCATCTTTTTCCGCAGATATGTAGCGGTTGGGAATGAGCCATTTTTGAAAGCTTACAATAATTCATTCTTGAACATCACCTTTCCTGCACTGCAGAACATTCAAAATGCCATCAATGAAGCTGGTCTTGGTGACACCGTAAAGGCAACAGTACCCTTAAACGCAGATGTCTATATGTCTCCGCAGGACAATCAAGTTCCATCTGCGGGAATATTTCGGCCCGATATCAATGGTCTCATGACTCAGATGGTACAATTTCTCAACAAGAACGGAGCACCATTTACTGTCAACATTTACCCCTTCTTAAGTCTCTACGGGAACGACGATTTCCCATTCAACTTCGCCTTCTTCGACGGGGTAGATAAACCAATAACTGACAATGGAATCCAATATACCAACGTCTTTGATGCAAATTTCGATACATTGGTTTCTGCTCTCAAATCGATCGGTTTCGGCGACACGCCAATTTTGGTAGGAGAAGTAGGATGGCCAACAGAAGGTGACAAGAATGCCAATTCCGGCAATGCCATAAGATTCTACAATGGCCTCCTTTCTAGACTCGCTGCGAACAAAGGAACCCCTCTCCGCCCCGGATTTATCGAAGTTTATCTGTTTGGACTCATCGATGAGGACGCGAAGAGCATTGCTCCAGGTAACTTTGAGAGACACTGGGGAATATTTCGATATGATGGACAACCGAAGTTTCCGATGGATCTTTCGGGTCAACTTCAAAACAAACTTCTAGTAGGTGCACAGGGTGTGAAATATCTTGATCCAATGTGGTGCATGTTTAACCCTGATGCTAAAGATCTCAGCAAACTTGCTGATAACATTAACTTTGCTTGCACCTTTGGAGATTGTACTGCACTTGGGTATGGATCTTCTTGCAACAACCTTGATGCTAATGGAAATGCTTCTTATGCATTCAACATGTACTACCAGATGCAGAATCAGGATCGTCAAGCATGCAATTTTGAAGGTTTAGCTAAGCCTACTACAGACAACATTTCAACACCTACTTGCAATTTTATCATCCAGATAGTTCCTTCTTCAGCATCTTCTTTGATGCCCTCATTTGCTGCTTTGTTGTTTATTGCTTTTTCTGTGATTCTATTGGCTTAG
Protein sequence

>MS.gene08225.t1

MGCFCCYAEGLGVNWGTQATHKLPPETVVKMLKDNGIQKVKLFDADDTTMTALAGSGIEVMVAIPNNQLAEMNDYKRAQYWVRKNVTRYNFDGGVKIKYVAVGNEPFLKAYNNSFLNITFPALQNIQNAINEAGLGDTVKATVPLNADVYMSPQDNQVPSAGIFRPDINGLMTQMVQFLNKNGAPFTVNIYPFLSLYGNDDFPFNFAFFDGVDKPITDNGIQYTNVFDANFDTLVSALKSIGFGDTPILVGEVGWPTEGDKNANSGNAIRFYNGLLSRLAANKGTPLRPGFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLSGQLQNKLLVGAQGVKYLDPMWCMFNPDAKDLSKLADNINFACTFGDCTALGYGSSCNNLDANGNASYAFNMYYQMQNQDRQACNFEGLAKPTTDNISTPTCNFIIQIVPSSASSLMPSFAALLFIAFSVILLA