AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene51889

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51889.t1 MTR_7g085390 99.355 465 3 0 1 465 16 480 0.0 948
MS.gene51889.t1 MTR_6g032820 80.569 422 79 3 1 419 16 437 0.0 694
MS.gene51889.t1 MTR_4g112025 57.609 460 188 3 5 459 27 484 0.0 543
MS.gene51889.t1 MTR_1g053320 55.921 456 199 2 11 464 30 485 0.0 524
MS.gene51889.t1 MTR_8g092070 54.503 433 194 2 8 439 31 461 2.28e-173 499
MS.gene51889.t1 MTR_1g010120 55.799 457 193 5 8 459 23 475 5.96e-173 494
MS.gene51889.t1 MTR_3g108600 52.941 459 186 5 6 456 26 462 1.69e-158 457
MS.gene51889.t1 MTR_3g095050 50.980 459 219 6 8 464 31 485 3.31e-157 454
MS.gene51889.t1 MTR_4g124440 51.562 448 208 5 10 453 27 469 2.60e-154 447
MS.gene51889.t1 MTR_2g027560 50.345 435 208 5 9 440 26 455 3.84e-154 447
MS.gene51889.t1 MTR_4g134280 49.326 371 175 5 99 462 2 366 1.66e-122 362
MS.gene51889.t1 MTR_3g031770 44.598 435 233 7 9 442 25 452 4.95e-118 355
MS.gene51889.t1 MTR_3g116510 33.941 439 264 18 11 435 28 454 9.95e-62 208
MS.gene51889.t1 MTR_1g031900 32.648 438 266 16 11 435 24 445 6.08e-61 207
MS.gene51889.t1 MTR_1g031900 32.648 438 266 16 11 435 24 445 1.50e-60 206
MS.gene51889.t1 MTR_4g069940 32.886 447 262 18 11 435 29 459 4.77e-58 198
MS.gene51889.t1 MTR_7g026340 34.529 446 257 20 8 435 20 448 8.76e-58 197
MS.gene51889.t1 MTR_5g085580 31.897 464 255 18 11 435 25 466 5.37e-57 196
MS.gene51889.t1 MTR_4g076255 34.663 326 192 9 9 331 23 330 1.65e-55 188
MS.gene51889.t1 MTR_6g088885 31.011 445 271 17 5 427 21 451 2.63e-55 192
MS.gene51889.t1 MTR_4g088155 30.580 448 275 19 11 435 23 457 9.20e-55 189
MS.gene51889.t1 MTR_4g076470 34.451 328 198 8 1 327 13 324 2.13e-54 185
MS.gene51889.t1 MTR_1g077990 34.731 334 197 11 8 332 28 349 1.59e-53 185
MS.gene51889.t1 MTR_4g076490 35.077 325 193 9 11 333 97 405 2.39e-53 185
MS.gene51889.t1 MTR_1g056640 30.258 466 271 19 11 435 38 490 1.13e-52 185
MS.gene51889.t1 MTR_4g076430 33.033 333 206 8 1 332 13 329 1.24e-52 180
MS.gene51889.t1 MTR_3g065460 30.023 443 271 16 11 435 24 445 1.43e-52 184
MS.gene51889.t1 MTR_4g076440 32.132 333 209 8 1 332 13 329 4.34e-52 179
MS.gene51889.t1 MTR_2g034480 32.335 334 214 6 11 342 27 350 7.07e-52 179
MS.gene51889.t1 MTR_1g022160 30.022 463 274 17 11 435 24 474 8.75e-52 182
MS.gene51889.t1 MTR_1g052430 33.851 322 197 9 10 327 31 340 1.52e-51 179
MS.gene51889.t1 MTR_1g007810 35.736 333 186 14 8 325 13 332 1.76e-51 178
MS.gene51889.t1 MTR_1g022160 29.935 461 273 17 11 433 24 472 5.42e-51 180
MS.gene51889.t1 MTR_4g010200 34.940 332 195 12 12 333 34 354 5.50e-51 177
MS.gene51889.t1 MTR_4g083500 28.842 475 292 15 4 449 37 494 2.39e-50 179
MS.gene51889.t1 MTR_1g022160 29.890 455 269 17 11 427 24 466 5.57e-50 177
MS.gene51889.t1 MTR_2g034440 33.645 321 200 7 11 328 22 332 7.09e-50 173
MS.gene51889.t1 MTR_2g034470 33.131 329 204 7 11 335 30 346 1.35e-49 173
MS.gene51889.t1 MTR_7g081350 32.615 325 199 11 10 329 26 335 3.79e-49 171
MS.gene51889.t1 MTR_4g114850 32.530 332 203 11 12 333 27 347 5.11e-49 172
MS.gene51889.t1 MTR_4g114850 32.530 332 203 11 12 333 27 347 9.72e-49 171
MS.gene51889.t1 MTR_4g114850 32.530 332 203 11 12 333 27 347 9.82e-49 171
MS.gene51889.t1 MTR_2g034470 33.758 314 192 7 26 335 10 311 1.31e-48 169
MS.gene51889.t1 MTR_5g078200 29.452 438 280 14 11 435 23 444 1.67e-48 176
MS.gene51889.t1 MTR_3g080410 34.969 326 187 12 9 322 24 336 1.81e-48 170
MS.gene51889.t1 MTR_8g012400 29.705 441 281 17 12 435 30 458 3.57e-48 172
MS.gene51889.t1 MTR_4g132280 29.318 440 278 15 11 435 45 466 8.43e-48 172
MS.gene51889.t1 MTR_4g076570 32.121 330 207 9 9 335 20 335 1.54e-47 167
MS.gene51889.t1 MTR_8g085720 29.571 443 274 17 11 435 26 448 1.89e-47 171
MS.gene51889.t1 MTR_3g083580 29.519 437 276 17 11 433 26 444 2.94e-47 170
MS.gene51889.t1 MTR_7g081370 32.615 325 188 11 9 329 26 323 9.82e-47 164
MS.gene51889.t1 MTR_4g076500 33.025 324 198 11 9 327 76 385 1.08e-46 166
MS.gene51889.t1 MTR_4g084480 32.845 341 208 12 2 333 24 352 1.19e-46 166
MS.gene51889.t1 MTR_4g084480 32.845 341 208 12 2 333 24 352 1.36e-46 166
MS.gene51889.t1 MTR_4g084480 32.845 341 208 12 2 333 17 345 1.52e-46 166
MS.gene51889.t1 MTR_4g052970 31.373 357 221 15 10 355 28 371 2.14e-46 165
MS.gene51889.t1 MTR_5g078200 29.245 424 271 14 25 435 4 411 5.31e-46 169
MS.gene51889.t1 MTR_2g086530 31.832 333 205 13 12 335 30 349 3.03e-44 159
MS.gene51889.t1 MTR_2g086530 31.832 333 205 13 12 335 9 328 3.50e-44 159
MS.gene51889.t1 MTR_3g116510 34.954 329 192 15 120 435 1 320 8.01e-44 157
MS.gene51889.t1 MTR_1g022160 32.036 334 207 11 11 334 24 347 4.97e-43 157
MS.gene51889.t1 MTR_2g019345 32.812 320 194 11 12 322 25 332 2.71e-42 154
MS.gene51889.t1 MTR_8g102340 30.682 352 223 12 78 419 62 402 5.61e-42 155
MS.gene51889.t1 MTR_7g053500 27.088 443 293 18 12 435 27 458 3.61e-41 153
MS.gene51889.t1 MTR_8g012400 29.679 374 235 16 78 435 17 378 8.79e-37 139
MS.gene51889.t1 MTR_7g053500 28.954 373 236 17 81 435 20 381 5.48e-34 132
MS.gene51889.t1 MTR_5g081720 27.778 324 212 13 28 342 54 364 8.56e-26 108
MS.gene51889.t1 MTR_4g036680 31.132 106 68 3 226 330 75 176 1.65e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51889.t1 AT1G64760 75.909 440 105 1 1 440 15 453 0.0 689
MS.gene51889.t1 AT1G64760 75.909 440 105 1 1 440 15 453 0.0 689
MS.gene51889.t1 AT2G19440 76.443 433 101 1 8 440 18 449 0.0 687
MS.gene51889.t1 AT2G19440 76.443 433 101 1 8 440 18 449 0.0 687
MS.gene51889.t1 AT3G04010 68.151 449 141 2 8 455 30 477 0.0 630
MS.gene51889.t1 AT5G18220 68.043 460 135 5 8 462 27 479 0.0 626
MS.gene51889.t1 AT3G24330 53.947 456 207 3 11 464 39 493 1.96e-175 501
MS.gene51889.t1 AT5G64790 56.979 437 185 2 8 443 25 459 5.44e-174 497
MS.gene51889.t1 AT4G31140 54.839 465 197 6 8 464 24 483 8.12e-173 494
MS.gene51889.t1 AT5G58090 53.275 458 207 5 5 458 16 470 1.16e-167 481
MS.gene51889.t1 AT5G20870 52.489 442 195 6 8 439 27 463 3.97e-150 437
MS.gene51889.t1 AT4G17180 48.864 440 212 5 9 446 23 451 1.18e-146 427
MS.gene51889.t1 AT5G58480 44.295 447 237 7 8 451 23 460 2.25e-124 370
MS.gene51889.t1 AT5G55180 33.182 440 269 15 11 435 27 456 1.56e-66 221
MS.gene51889.t1 AT5G55180 33.803 426 257 15 11 421 27 442 2.58e-66 220
MS.gene51889.t1 AT2G16230 33.790 438 264 16 11 435 25 449 2.72e-65 218
MS.gene51889.t1 AT2G05790 33.333 462 253 21 11 435 25 468 4.83e-64 214
MS.gene51889.t1 AT3G55430 34.240 441 260 17 8 435 26 449 1.56e-63 213
MS.gene51889.t1 AT2G39640 33.105 438 257 17 8 433 27 440 2.83e-59 204
MS.gene51889.t1 AT4G26830 32.045 440 271 16 11 435 25 451 5.80e-59 201
MS.gene51889.t1 AT4G26830 32.273 440 270 17 11 435 29 455 7.76e-59 200
MS.gene51889.t1 AT2G01630 31.111 450 274 17 11 446 23 450 3.44e-58 199
MS.gene51889.t1 AT2G01630 30.716 433 269 16 11 429 23 438 7.47e-57 194
MS.gene51889.t1 AT3G13560 33.108 444 267 15 5 435 20 446 8.95e-57 196
MS.gene51889.t1 AT3G13560 33.108 444 267 15 5 435 20 446 8.95e-57 196
MS.gene51889.t1 AT3G13560 33.108 444 267 15 5 435 20 446 8.95e-57 196
MS.gene51889.t1 AT3G13560 33.108 444 267 15 5 435 20 446 8.95e-57 196
MS.gene51889.t1 AT3G13560 33.108 444 267 15 5 435 20 446 8.95e-57 196
MS.gene51889.t1 AT4G34480 33.030 439 263 17 11 435 26 447 1.62e-56 195
MS.gene51889.t1 AT3G07320 32.200 441 271 18 12 435 26 455 2.58e-56 194
MS.gene51889.t1 AT1G66250 30.543 442 269 15 11 435 32 452 6.65e-56 194
MS.gene51889.t1 AT5G24318 29.464 448 273 18 10 435 29 455 3.68e-54 188
MS.gene51889.t1 AT1G11820 30.023 443 271 16 11 435 44 465 1.01e-53 188
MS.gene51889.t1 AT4G14080 31.236 445 273 20 11 435 42 473 1.74e-53 186
MS.gene51889.t1 AT4G16260 32.722 327 206 7 9 333 22 336 1.89e-53 183
MS.gene51889.t1 AT5G24318 29.680 438 265 18 10 425 29 445 1.89e-53 186
MS.gene51889.t1 AT5G56590 29.075 454 281 17 5 436 20 454 1.14e-52 185
MS.gene51889.t1 AT1G11820 29.977 437 267 16 11 429 44 459 1.71e-52 184
MS.gene51889.t1 AT3G23770 31.696 448 270 21 11 435 37 471 2.48e-52 183
MS.gene51889.t1 AT4G16260 33.228 316 196 8 21 333 4 307 2.56e-52 179
MS.gene51889.t1 AT3G57270 33.951 324 200 9 11 331 27 339 3.53e-51 177
MS.gene51889.t1 AT2G26600 31.461 356 220 12 6 348 29 373 7.05e-50 175
MS.gene51889.t1 AT5G42720 35.514 321 189 11 11 322 26 337 2.63e-49 174
MS.gene51889.t1 AT1G30080 32.424 330 203 10 2 322 27 345 3.01e-49 173
MS.gene51889.t1 AT3G57260 33.333 324 196 9 11 331 32 338 4.12e-49 171
MS.gene51889.t1 AT3G57260 32.836 335 204 10 1 331 37 354 5.69e-49 171
MS.gene51889.t1 AT1G30080 32.424 330 203 10 2 322 27 345 5.80e-49 173
MS.gene51889.t1 AT3G15800 32.647 340 207 12 5 333 37 365 1.49e-48 171
MS.gene51889.t1 AT5G42100 35.119 336 195 13 8 331 24 348 7.23e-48 170
MS.gene51889.t1 AT5G42100 35.119 336 195 13 8 331 24 348 1.34e-47 169
MS.gene51889.t1 AT4G34480 36.533 323 182 14 11 322 26 336 1.96e-47 167
MS.gene51889.t1 AT1G32860 33.538 325 192 12 11 323 29 341 9.56e-47 167
MS.gene51889.t1 AT2G01630 31.138 334 209 11 11 333 23 346 5.80e-46 164
MS.gene51889.t1 AT5G24318 31.003 329 201 12 10 327 29 342 1.57e-44 160
MS.gene51889.t1 AT3G57240 31.707 328 201 11 4 327 28 336 2.09e-44 159
MS.gene51889.t1 AT4G29360 26.407 462 294 15 11 437 37 487 1.30e-43 161
MS.gene51889.t1 AT4G29360 26.407 462 294 15 11 437 27 477 1.59e-43 160
MS.gene51889.t1 AT4G18340 30.792 341 214 12 2 332 25 353 5.14e-43 156
MS.gene51889.t1 AT4G18340 31.024 332 207 12 2 323 25 344 9.20e-43 156
MS.gene51889.t1 AT1G11820 30.030 333 214 10 11 333 44 367 2.92e-42 154
MS.gene51889.t1 AT3G61810 28.869 336 207 12 11 332 55 372 6.43e-40 147
MS.gene51889.t1 AT2G27500 29.377 337 217 11 9 335 27 352 9.02e-40 147
MS.gene51889.t1 AT2G27500 28.933 356 230 13 9 353 27 370 1.59e-39 147
MS.gene51889.t1 AT4G34480 32.665 349 207 15 100 435 3 336 3.66e-39 146
MS.gene51889.t1 AT3G46570 30.275 327 205 14 11 327 27 340 3.28e-37 140
MS.gene51889.t1 AT2G26600 30.714 280 171 11 81 348 11 279 2.16e-34 130
MS.gene51889.t1 AT1G77780 29.412 323 208 9 12 329 25 332 2.63e-34 132
MS.gene51889.t1 AT5G24318 29.641 334 196 15 123 435 1 316 8.35e-34 129
MS.gene51889.t1 AT5G20390 28.529 333 216 11 4 329 26 343 1.13e-32 127
MS.gene51889.t1 AT5G20340 27.329 322 213 10 4 319 36 342 2.83e-30 120
MS.gene51889.t1 AT3G55780 26.000 350 229 12 11 342 31 368 7.02e-30 120
MS.gene51889.t1 AT5G20330 26.708 322 215 10 4 319 27 333 9.31e-30 119
MS.gene51889.t1 AT1G77790 28.939 311 198 11 25 327 44 339 1.14e-29 119
MS.gene51889.t1 AT1G33220 27.597 308 205 9 4 305 27 322 1.57e-29 118
MS.gene51889.t1 AT2G27500 29.123 285 181 11 61 335 1 274 4.75e-29 116
MS.gene51889.t1 AT5G20560 26.984 315 210 10 11 319 34 334 4.23e-28 114

Find 113 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATATAGACATCTGCGTTTA+AGG 0.135341 7.4:+21966722 None:intergenic
CATCAAAGACGTTGGTATAT+TGG 0.201622 7.4:+21966490 None:intergenic
ATTATAAACGTGCTCAATAT+TGG 0.203608 7.4:-21968355 MS.gene51889:CDS
AACATCTTCACAACAGTTTC+TGG 0.211606 7.4:+21968503 None:intergenic
TGGATTCCATTGTCAGTTAT+TGG 0.212683 7.4:+21966510 None:intergenic
ATTTCAGCAAGTTGATTATT+AGG 0.225555 7.4:+21968383 None:intergenic
CCTACTTCTCCTACCAAAAT+TGG 0.271582 7.4:+21966414 None:intergenic
TGCAACAACCTTGATGCTAA+TGG 0.283164 7.4:-21965991 MS.gene51889:CDS
TTTCGGCGACACGCCAATTT+TGG 0.288643 7.4:-21966427 MS.gene51889:CDS
AAGTTTCCGATGGATCTTTC+GGG 0.294935 7.4:-21966177 MS.gene51889:CDS
GCTCTCAAATCGATCGGTTT+CGG 0.295941 7.4:-21966444 MS.gene51889:CDS
TAAGGGTACTGTTGCCTTTA+CGG 0.296654 7.4:+21966740 None:intergenic
TGGAAGGGCTTGGTGTAAAC+TGG 0.297579 7.4:-21968553 MS.gene51889:CDS
ATTAACTTTGCTTGCACCTT+TGG 0.307246 7.4:-21966045 MS.gene51889:CDS
TTCATCGAAGTTTATCTGTT+TGG 0.307651 7.4:-21966282 MS.gene51889:CDS
TCTTATTGCATTGCCGGAAT+TGG 0.311120 7.4:+21966365 None:intergenic
GTAGAATCTTATTGCATTGC+CGG 0.319258 7.4:+21966359 None:intergenic
GGGTAAATGTTGACAGTAAA+TGG 0.333092 7.4:+21966600 None:intergenic
TTCCATCTGCGGGAATATTT+CGG 0.334659 7.4:-21966680 MS.gene51889:CDS
CAGATAAACTTCGATGAATC+CGG 0.350042 7.4:+21966287 None:intergenic
TTATAAACGTGCTCAATATT+GGG 0.352564 7.4:-21968354 MS.gene51889:CDS
GTGAAGATGTTGAAGGATAA+TGG 0.352574 7.4:-21968491 MS.gene51889:CDS
GTTACTCGTTATAACTTTGA+TGG 0.354819 7.4:-21968320 MS.gene51889:CDS
TGTTGTTATGTGGAAGGGCT+TGG 0.368756 7.4:-21968563 MS.gene51889:CDS
GGTGACAAGAATGCCAATTC+CGG 0.398140 7.4:-21966378 MS.gene51889:CDS
GAAGTTTCCGATGGATCTTT+CGG 0.401121 7.4:-21966178 MS.gene51889:CDS
TTGCTGAGATCTTTAGCATC+AGG 0.401772 7.4:+21966081 None:intergenic
TCGAAGAAGGCGAAGTTGAA+TGG 0.408365 7.4:+21966546 None:intergenic
AGTCATGAGACCATTGATAT+CGG 0.412963 7.4:+21966656 None:intergenic
TCAACTTCGCCTTCTTCGAC+GGG 0.422981 7.4:-21966542 MS.gene51889:CDS
TGAAGATGTTGAAGGATAAT+GGG 0.423140 7.4:-21968490 MS.gene51889:CDS
CGATGAATCCGGGGCGGAGA+GGG 0.424811 7.4:+21966298 None:intergenic
TTCAACTTCGCCTTCTTCGA+CGG 0.430662 7.4:-21966543 MS.gene51889:CDS
GACGATCCTGATTCTGCGTC+TGG 0.432976 7.4:+21965938 None:intergenic
GTACTACCAGACGCAGAATC+AGG 0.436349 7.4:-21965944 MS.gene51889:CDS
ACCAAGACCAGCTTCATTGA+TGG 0.438757 7.4:+21966767 None:intergenic
TTTATCTACCCCGTCGAAGA+AGG 0.451188 7.4:+21966533 None:intergenic
TCCGCAGATATGTAGCGGTT+GGG 0.453302 7.4:-21966866 MS.gene51889:intron
TACCCCTTCTTAAGTCTCTA+CGG 0.454310 7.4:-21966582 MS.gene51889:CDS
ATGCTGAAGAAGGAACTATC+TGG 0.458076 7.4:+21965842 None:intergenic
TGATACTACAATGACTGCTT+TGG 0.463045 7.4:-21968438 MS.gene51889:CDS
CTTCAAAACAAACTTCTAGT+AGG 0.464131 7.4:-21966150 MS.gene51889:CDS
GCATTCTTGTCACCTTCTGT+TGG 0.465889 7.4:+21966387 None:intergenic
AAGCATTTCCATTAGCATCA+AGG 0.472386 7.4:+21965983 None:intergenic
CGAAGAAGGCGAAGTTGAAT+GGG 0.474909 7.4:+21966547 None:intergenic
TTCCGCAGATATGTAGCGGT+TGG 0.484860 7.4:-21966867 MS.gene51889:intron
ACCCCTTCTTAAGTCTCTAC+GGG 0.484918 7.4:-21966581 MS.gene51889:CDS
CAGTGTCTCTCAAAGTTACC+TGG 0.486453 7.4:+21966228 None:intergenic
GATTGTACTGCACTTGGGTA+TGG 0.490793 7.4:-21966021 MS.gene51889:CDS
TGATGCAAATTTCGATACAT+TGG 0.491804 7.4:-21966472 MS.gene51889:CDS
AAACAACAAAGCAGCAAATG+AGG 0.493412 7.4:+21965807 None:intergenic
TGGGGAATATTTCGATATGA+TGG 0.495745 7.4:-21966207 MS.gene51889:CDS
TGTTCGCAGCGAGTCTAGAA+AGG 0.496041 7.4:+21966328 None:intergenic
GCCATCAATGAAGCTGGTCT+TGG 0.496624 7.4:-21966768 MS.gene51889:CDS
AACGTGCTCAATATTGGGTT+AGG 0.498245 7.4:-21968349 MS.gene51889:CDS
GTTGAAATGTTGTCTGTAGT+AGG 0.500081 7.4:+21965886 None:intergenic
ATGACTGCTTTGGCTGGTAG+TGG 0.501400 7.4:-21968428 MS.gene51889:CDS
TGGTAGTGGAATTGAAGTTA+TGG 0.502034 7.4:-21968414 MS.gene51889:CDS
TTTGGAGATTGTACTGCACT+TGG 0.515034 7.4:-21966027 MS.gene51889:CDS
GTCATGAGACCATTGATATC+GGG 0.521177 7.4:+21966657 None:intergenic
GTTTCTGCTCTCAAATCGAT+CGG 0.522943 7.4:-21966450 MS.gene51889:CDS
CAAAATGCCATCAATGAAGC+TGG 0.527897 7.4:-21966774 MS.gene51889:CDS
GGCCGAAATATTCCCGCAGA+TGG 0.529991 7.4:+21966678 None:intergenic
ATCAAAGAAGATGCTGAAGA+AGG 0.530104 7.4:+21965832 None:intergenic
AGATAAACTTCGATGAATCC+GGG 0.532252 7.4:+21966288 None:intergenic
TGCTGAGATCTTTAGCATCA+GGG 0.538134 7.4:+21966082 None:intergenic
CAAACTTCTAGTAGGTGCAC+AGG 0.538210 7.4:-21966142 MS.gene51889:CDS
ATATAGACATCTGCGTTTAA+GGG 0.538392 7.4:+21966723 None:intergenic
CAGGTAACTTTGAGAGACAC+TGG 0.539845 7.4:-21966227 MS.gene51889:CDS
GACAATCAAGTTCCATCTGC+GGG 0.543282 7.4:-21966690 MS.gene51889:CDS
GATGAATCCGGGGCGGAGAG+GGG 0.546532 7.4:+21966299 None:intergenic
GACGCGAAGAGCATTGCTCC+AGG 0.552654 7.4:-21966246 MS.gene51889:CDS
ACTACAATGACTGCTTTGGC+TGG 0.558501 7.4:-21968434 MS.gene51889:CDS
ATATTTCGGCCCGATATCAA+TGG 0.563489 7.4:-21966666 MS.gene51889:CDS
TCGATGAATCCGGGGCGGAG+AGG 0.565244 7.4:+21966297 None:intergenic
TTGGAGATTGTACTGCACTT+GGG 0.571116 7.4:-21966026 MS.gene51889:CDS
AACTGTTGTGAAGATGTTGA+AGG 0.573217 7.4:-21968498 MS.gene51889:CDS
GATAAACCAATAACTGACAA+TGG 0.575139 7.4:-21966516 MS.gene51889:CDS
TCTAGACTCGCTGCGAACAA+AGG 0.575607 7.4:-21966324 MS.gene51889:CDS
AAAGGAACCCCTCTCCGCCC+CGG 0.576993 7.4:-21966306 MS.gene51889:CDS
GAAAGATCCATCGGAAACTT+CGG 0.579080 7.4:+21966180 None:intergenic
GAAGTAGGATGGCCAACAGA+AGG 0.581640 7.4:-21966399 MS.gene51889:CDS
GGAAGGGCTTGGTGTAAACT+GGG 0.581663 7.4:-21968552 MS.gene51889:CDS
TGGTCTTGGTGACACCGTAA+AGG 0.581918 7.4:-21966754 MS.gene51889:CDS
AATGTTCTGCAGTGCAGGAA+AGG 0.584113 7.4:+21966797 None:intergenic
CAATGGTCTCATGACTCAGA+TGG 0.589085 7.4:-21966649 MS.gene51889:CDS
GAAATTTGCATCAAAGACGT+TGG 0.589177 7.4:+21966482 None:intergenic
AGGTAACTTTGAGAGACACT+GGG 0.591795 7.4:-21966226 MS.gene51889:CDS
GTTCCCGTAGAGACTTAAGA+AGG 0.603438 7.4:+21966578 None:intergenic
AACAACAAAGCAGCAAATGA+GGG 0.604408 7.4:+21965808 None:intergenic
AGATGTCTATATGTCTCCGC+AGG 0.611737 7.4:-21966712 MS.gene51889:CDS
ACTCGTTATAACTTTGATGG+CGG 0.617236 7.4:-21968317 MS.gene51889:CDS
GGGTTAAACATGCACCACAT+TGG 0.618943 7.4:+21966102 None:intergenic
TGGATGATAAAATTGCAAGT+AGG 0.623501 7.4:+21965862 None:intergenic
TTCCCGTAGAGACTTAAGAA+GGG 0.632332 7.4:+21966579 None:intergenic
GTACAATTTCTCAACAAGAA+CGG 0.637978 7.4:-21966627 MS.gene51889:CDS
ATCTTCACAACAGTTTCTGG+TGG 0.639053 7.4:+21968506 None:intergenic
AGTTGACCCGAAAGATCCAT+CGG 0.649595 7.4:+21966171 None:intergenic
TCCCGTAGAGACTTAAGAAG+GGG 0.652260 7.4:+21966580 None:intergenic
GGACAATCAAGTTCCATCTG+CGG 0.652978 7.4:-21966691 MS.gene51889:CDS
AAGTGCAGTACAATCTCCAA+AGG 0.655496 7.4:+21966029 None:intergenic
TGGACAACCGAAGTTTCCGA+TGG 0.662375 7.4:-21966187 MS.gene51889:CDS
AAACTTCTAGTAGGTGCACA+GGG 0.662815 7.4:-21966141 MS.gene51889:CDS
TCTGTTTGGACTCATCGATG+AGG 0.680701 7.4:-21966268 MS.gene51889:CDS
TCGCAGCGAGTCTAGAAAGG+AGG 0.683390 7.4:+21966331 None:intergenic
GATAAACTTCGATGAATCCG+GGG 0.689389 7.4:+21966289 None:intergenic
AATGCAATAAGATTCTACAA+TGG 0.690889 7.4:-21966354 MS.gene51889:CDS
TGAAATATCTTGATCCAATG+TGG 0.694528 7.4:-21966116 MS.gene51889:CDS
CAACTTCGCCTTCTTCGACG+GGG 0.696178 7.4:-21966541 MS.gene51889:CDS
GATGGAACTTGATTGTCCTG+CGG 0.701807 7.4:+21966696 None:intergenic
AAACTTCGATGAATCCGGGG+CGG 0.742113 7.4:+21966292 None:intergenic
GAAGGGCTTGGTGTAAACTG+GGG 0.765857 7.4:-21968551 MS.gene51889:CDS
GGTAACTTTGAGAGACACTG+GGG 0.786507 7.4:-21966225 MS.gene51889:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATATTTTTATTTTTGTCA+GGG - chr7.4:21966374-21966393 MS.gene51889:CDS 10.0%
!!! TTTATATTTTTATTTTTGTC+AGG - chr7.4:21966373-21966392 MS.gene51889:CDS 10.0%
!! AATAAAAGAATCTAATTCTG+AGG + chr7.4:21966583-21966602 None:intergenic 20.0%
!! AATCATTATTATGAACACAA+AGG + chr7.4:21966431-21966450 None:intergenic 20.0%
!! ATAAAAGAATCTAATTCTGA+GGG + chr7.4:21966582-21966601 None:intergenic 20.0%
!! ATCATTATTATGAACACAAA+GGG + chr7.4:21966430-21966449 None:intergenic 20.0%
!! TTATTGTAAGCTTTCAAAAA+TGG + chr7.4:21967518-21967537 None:intergenic 20.0%
!!! GATTTTATTGAACATTTTGA+AGG - chr7.4:21967112-21967131 MS.gene51889:intron 20.0%
!!! TCAGAATTAGATTCTTTTAT+TGG - chr7.4:21966582-21966601 MS.gene51889:CDS 20.0%
!!! TTTGTTAGATTTTTGATTCA+TGG - chr7.4:21966339-21966358 MS.gene51889:CDS 20.0%
! AATGCAATAAGATTCTACAA+TGG - chr7.4:21967998-21968017 MS.gene51889:intron 25.0%
! AGAACAAATACATTATACCT+CGG + chr7.4:21966650-21966669 None:intergenic 25.0%
! ATGTGCATATATCATATTCT+TGG - chr7.4:21966748-21966767 MS.gene51889:CDS 25.0%
! ATTATAAACGTGCTCAATAT+TGG - chr7.4:21965997-21966016 MS.gene51889:CDS 25.0%
! ATTCATCAAAGCTTAAAATG+TGG + chr7.4:21967163-21967182 None:intergenic 25.0%
! GCACAAATTCACATTAAAAT+CGG + chr7.4:21967450-21967469 None:intergenic 25.0%
! TGGTGTTTAATAATAAGAAG+CGG + chr7.4:21967143-21967162 None:intergenic 25.0%
! TTATAAACGTGCTCAATATT+GGG - chr7.4:21965998-21966017 MS.gene51889:CDS 25.0%
! TTGACAACACTAGATTTAAT+GGG - chr7.4:21967090-21967109 MS.gene51889:intron 25.0%
! TTTGACAACACTAGATTTAA+TGG - chr7.4:21967089-21967108 MS.gene51889:intron 25.0%
!! GTCTGTTCAAACAATTTTAA+AGG - chr7.4:21966946-21966965 MS.gene51889:intron 25.0%
!! TATTTTTCACAATCAGCAAT+TGG + chr7.4:21967034-21967053 None:intergenic 25.0%
!! TTAGATTCTTTTATTGGTGT+TGG - chr7.4:21966588-21966607 MS.gene51889:CDS 25.0%
!! TTTTCCTTCTGTATTATAGT+TGG - chr7.4:21966913-21966932 MS.gene51889:intron 25.0%
!!! AATTTTTCGAGATTTTGTGT+TGG - chr7.4:21966177-21966196 MS.gene51889:CDS 25.0%
!!! AGTTGTTCTTGTTTTAATCA+AGG - chr7.4:21966534-21966553 MS.gene51889:CDS 25.0%
!!! ATTTCAGCAAGTTGATTATT+AGG + chr7.4:21965972-21965991 None:intergenic 25.0%
!!! TAGATTTTTGATTCATGGTT+AGG - chr7.4:21966344-21966363 MS.gene51889:CDS 25.0%
!!! TGATTATGCATTTTGATTGA+TGG - chr7.4:21966452-21966471 MS.gene51889:CDS 25.0%
!!! TTCCTTGTAGTAACTTTTTT+CGG - chr7.4:21966622-21966641 MS.gene51889:CDS 25.0%
AAATCTAGTGTTGTCAAAAG+CGG + chr7.4:21967087-21967106 None:intergenic 30.0%
ATATAGACATCTGCGTTTAA+GGG + chr7.4:21967632-21967651 None:intergenic 30.0%
CAACCAGCATTACAAATTAT+TGG + chr7.4:21967274-21967293 None:intergenic 30.0%
CATTAAAATCGGTCGAAATT+CGG + chr7.4:21967439-21967458 None:intergenic 30.0%
CTTCAAAACAAACTTCTAGT+AGG - chr7.4:21968202-21968221 MS.gene51889:intron 30.0%
GATAAACCAATAACTGACAA+TGG - chr7.4:21967836-21967855 MS.gene51889:intron 30.0%
GCATATATCATATTCTTGGA+AGG - chr7.4:21966752-21966771 MS.gene51889:CDS 30.0%
GTACAATTTCTCAACAAGAA+CGG - chr7.4:21967725-21967744 MS.gene51889:intron 30.0%
GTTACTCGTTATAACTTTGA+TGG - chr7.4:21966032-21966051 MS.gene51889:CDS 30.0%
TCAACCAACTATAATACAGA+AGG + chr7.4:21966920-21966939 None:intergenic 30.0%
TGAAATATCTTGATCCAATG+TGG - chr7.4:21968236-21968255 MS.gene51889:intron 30.0%
TGAAGATGTTGAAGGATAAT+GGG - chr7.4:21965862-21965881 MS.gene51889:CDS 30.0%
TGATGCAAATTTCGATACAT+TGG - chr7.4:21967880-21967899 MS.gene51889:intron 30.0%
TGATTGAGAGTTCATATAAC+AGG - chr7.4:21966829-21966848 MS.gene51889:CDS 30.0%
TGGATGATAAAATTGCAAGT+AGG + chr7.4:21968493-21968512 None:intergenic 30.0%
TTCATCGAAGTTTATCTGTT+TGG - chr7.4:21968070-21968089 MS.gene51889:intron 30.0%
! AATCATTTTACCATTCTAGG+AGG - chr7.4:21966673-21966692 MS.gene51889:CDS 30.0%
! GAGAATCATTTTACCATTCT+AGG - chr7.4:21966670-21966689 MS.gene51889:CDS 30.0%
! GCTTGTAGAGATTTTATTGA+TGG - chr7.4:21966981-21967000 MS.gene51889:intron 30.0%
! TAACAGGAAATTTTGCATCA+AGG - chr7.4:21966845-21966864 MS.gene51889:CDS 30.0%
! TAGCCAATAATTTGTAATGC+TGG - chr7.4:21967268-21967287 MS.gene51889:intron 30.0%
!! AAAGATGGCATTGATGAATT+TGG + chr7.4:21967368-21967387 None:intergenic 30.0%
!! AAGATGGCATTGATGAATTT+GGG + chr7.4:21967367-21967386 None:intergenic 30.0%
!!! GTTTTTGTTGTTATGTGGAA+GGG - chr7.4:21965784-21965803 MS.gene51889:CDS 30.0%
!!! TGCTTTTTCTGTGATTCTAT+TGG - chr7.4:21968570-21968589 MS.gene51889:CDS 30.0%
!!! TGTTTTTGTTGTTATGTGGA+AGG - chr7.4:21965783-21965802 MS.gene51889:CDS 30.0%
AAACAACAAAGCAGCAAATG+AGG + chr7.4:21968548-21968567 None:intergenic 35.0%
AACAACAAAGCAGCAAATGA+GGG + chr7.4:21968547-21968566 None:intergenic 35.0%
AACATCTTCACAACAGTTTC+TGG + chr7.4:21965852-21965871 None:intergenic 35.0%
AACTGTTGTGAAGATGTTGA+AGG - chr7.4:21965854-21965873 MS.gene51889:CDS 35.0%
AAGCATTTCCATTAGCATCA+AGG + chr7.4:21968372-21968391 None:intergenic 35.0%
ACTCGTTATAACTTTGATGG+CGG - chr7.4:21966035-21966054 MS.gene51889:CDS 35.0%
AGATAAACTTCGATGAATCC+GGG + chr7.4:21968067-21968086 None:intergenic 35.0%
AGTCATGAGACCATTGATAT+CGG + chr7.4:21967699-21967718 None:intergenic 35.0%
ATCAAAGAAGATGCTGAAGA+AGG + chr7.4:21968523-21968542 None:intergenic 35.0%
ATTAACTTTGCTTGCACCTT+TGG - chr7.4:21968307-21968326 MS.gene51889:CDS 35.0%
CACCGAAAAAAGTTACTACA+AGG + chr7.4:21966627-21966646 None:intergenic 35.0%
CAGATAAACTTCGATGAATC+CGG + chr7.4:21968068-21968087 None:intergenic 35.0%
CATATAGACATCTGCGTTTA+AGG + chr7.4:21967633-21967652 None:intergenic 35.0%
CATCAAAGACGTTGGTATAT+TGG + chr7.4:21967865-21967884 None:intergenic 35.0%
CCAATCCAATGACAAAAACA+AGG + chr7.4:21967312-21967331 None:intergenic 35.0%
GAAATTTGCATCAAAGACGT+TGG + chr7.4:21967873-21967892 None:intergenic 35.0%
GGGTAAATGTTGACAGTAAA+TGG + chr7.4:21967755-21967774 None:intergenic 35.0%
GTAGAATCTTATTGCATTGC+CGG + chr7.4:21967996-21968015 None:intergenic 35.0%
GTATAATGTGCTTGCTTCAT+TGG - chr7.4:21966222-21966241 MS.gene51889:CDS 35.0%
GTGAAGATGTTGAAGGATAA+TGG - chr7.4:21965861-21965880 MS.gene51889:CDS 35.0%
GTTGAAATGTTGTCTGTAGT+AGG + chr7.4:21968469-21968488 None:intergenic 35.0%
TACATATCTGCGGAAAAAGA+TGG + chr7.4:21967480-21967499 None:intergenic 35.0%
TCATCAATGCCATCTTTAGT+TGG - chr7.4:21967371-21967390 MS.gene51889:intron 35.0%
TGAAGCAAGCACATTATACA+AGG + chr7.4:21966221-21966240 None:intergenic 35.0%
TGATACTACAATGACTGCTT+TGG - chr7.4:21965914-21965933 MS.gene51889:CDS 35.0%
TGGATTCCATTGTCAGTTAT+TGG + chr7.4:21967845-21967864 None:intergenic 35.0%
TGGGGAATATTTCGATATGA+TGG - chr7.4:21968145-21968164 MS.gene51889:intron 35.0%
TTGGTCAAACACTAAAATGG+TGG - chr7.4:21967328-21967347 MS.gene51889:intron 35.0%
! ATCAAGGAAGCTTTATGTAC+TGG - chr7.4:21966550-21966569 MS.gene51889:CDS 35.0%
! TCAAGGAAGCTTTATGTACT+GGG - chr7.4:21966551-21966570 MS.gene51889:CDS 35.0%
!! AGATGGCATTGATGAATTTG+GGG + chr7.4:21967366-21967385 None:intergenic 35.0%
!! GGATTGGTCAAACACTAAAA+TGG - chr7.4:21967325-21967344 MS.gene51889:intron 35.0%
!! TGGTAGTGGAATTGAAGTTA+TGG - chr7.4:21965938-21965957 MS.gene51889:CDS 35.0%
!!! CCTTGTTTTTGTCATTGGAT+TGG - chr7.4:21967309-21967328 MS.gene51889:intron 35.0%
!!! GGGTTGTTTTTGTTGTTATG+TGG - chr7.4:21965779-21965798 MS.gene51889:CDS 35.0%
!!! TTTGATTGATGGCGAGTTTT+GGG - chr7.4:21966463-21966482 MS.gene51889:CDS 35.0%
!!! TTTTGATTGATGGCGAGTTT+TGG - chr7.4:21966462-21966481 MS.gene51889:CDS 35.0%
AAACTTCTAGTAGGTGCACA+GGG - chr7.4:21968211-21968230 MS.gene51889:intron 40.0%
AACGTGCTCAATATTGGGTT+AGG - chr7.4:21966003-21966022 MS.gene51889:CDS 40.0%
AAGTGCAGTACAATCTCCAA+AGG + chr7.4:21968326-21968345 None:intergenic 40.0%
AAGTTTCCGATGGATCTTTC+GGG - chr7.4:21968175-21968194 MS.gene51889:intron 40.0%
ATATTTCGGCCCGATATCAA+TGG - chr7.4:21967686-21967705 MS.gene51889:intron 40.0%
ATGCTGAAGAAGGAACTATC+TGG + chr7.4:21968513-21968532 None:intergenic 40.0%
ATTCAATTGTAGCCCTGCTA+TGG - chr7.4:21967061-21967080 MS.gene51889:intron 40.0%
CAAAATGCCATCAATGAAGC+TGG - chr7.4:21967578-21967597 MS.gene51889:intron 40.0%
CCTACTTCTCCTACCAAAAT+TGG + chr7.4:21967941-21967960 None:intergenic 40.0%
CTACTGCTTCCAACTAAAGA+TGG + chr7.4:21967383-21967402 None:intergenic 40.0%
GAAAGATCCATCGGAAACTT+CGG + chr7.4:21968175-21968194 None:intergenic 40.0%
GAAGTTTCCGATGGATCTTT+CGG - chr7.4:21968174-21968193 MS.gene51889:intron 40.0%
GATAAACTTCGATGAATCCG+GGG + chr7.4:21968066-21968085 None:intergenic 40.0%
GTCATGAGACCATTGATATC+GGG + chr7.4:21967698-21967717 None:intergenic 40.0%
GTTTCTGCTCTCAAATCGAT+CGG - chr7.4:21967902-21967921 MS.gene51889:intron 40.0%
TACCCCTTCTTAAGTCTCTA+CGG - chr7.4:21967770-21967789 MS.gene51889:intron 40.0%
TCTTATTGCATTGCCGGAAT+TGG + chr7.4:21967990-21968009 None:intergenic 40.0%
TGCAACAACCTTGATGCTAA+TGG - chr7.4:21968361-21968380 MS.gene51889:CDS 40.0%
TGCTGAGATCTTTAGCATCA+GGG + chr7.4:21968273-21968292 None:intergenic 40.0%
TTCCATCTGCGGGAATATTT+CGG - chr7.4:21967672-21967691 MS.gene51889:intron 40.0%
TTCCCGTAGAGACTTAAGAA+GGG + chr7.4:21967776-21967795 None:intergenic 40.0%
TTGCTGAGATCTTTAGCATC+AGG + chr7.4:21968274-21968293 None:intergenic 40.0%
TTGGAGATTGTACTGCACTT+GGG - chr7.4:21968326-21968345 MS.gene51889:CDS 40.0%
TTTGGAGATTGTACTGCACT+TGG - chr7.4:21968325-21968344 MS.gene51889:CDS 40.0%
! AGGTAACTTTGAGAGACACT+GGG - chr7.4:21968126-21968145 MS.gene51889:intron 40.0%
! ATCTTCACAACAGTTTCTGG+TGG + chr7.4:21965849-21965868 None:intergenic 40.0%
! CGTCAAGCATGCAATTTTGA+AGG - chr7.4:21968433-21968452 MS.gene51889:CDS 40.0%
! CTTTTTCCGCAGATATGTAG+CGG - chr7.4:21967481-21967500 MS.gene51889:intron 40.0%
! TTTTGAATGTTCTGCAGTGC+AGG + chr7.4:21967563-21967582 None:intergenic 40.0%
! TTTTGCATGTGCGCGTATAT+GGG - chr7.4:21966791-21966810 MS.gene51889:CDS 40.0%
! TTTTGGTAGGAGAAGTAGGA+TGG - chr7.4:21967942-21967961 MS.gene51889:intron 40.0%
! TTTTTGCATGTGCGCGTATA+TGG - chr7.4:21966790-21966809 MS.gene51889:CDS 40.0%
!! CCAATTTTGGTAGGAGAAGT+AGG - chr7.4:21967938-21967957 MS.gene51889:intron 40.0%
!! TAAGGGTACTGTTGCCTTTA+CGG + chr7.4:21967615-21967634 None:intergenic 40.0%
!!! AGGAGGTGATTGCTGTTTTA+GGG - chr7.4:21966690-21966709 MS.gene51889:CDS 40.0%
!!! CTGTTTTAGGGGAAGAGAAA+TGG - chr7.4:21966702-21966721 MS.gene51889:CDS 40.0%
!!! TAGGAGGTGATTGCTGTTTT+AGG - chr7.4:21966689-21966708 MS.gene51889:CDS 40.0%
!!! TGTCGCCTTGTTTTTGTCAT+TGG - chr7.4:21967304-21967323 MS.gene51889:intron 40.0%
AAATCGGTCGAAATTCGGCA+AGG + chr7.4:21967434-21967453 None:intergenic 45.0%
AATGTTCTGCAGTGCAGGAA+AGG + chr7.4:21967558-21967577 None:intergenic 45.0%
ACAGCAATCACCTCCTAGAA+TGG + chr7.4:21966686-21966705 None:intergenic 45.0%
ACCAAGACCAGCTTCATTGA+TGG + chr7.4:21967588-21967607 None:intergenic 45.0%
ACCCCTTCTTAAGTCTCTAC+GGG - chr7.4:21967771-21967790 MS.gene51889:intron 45.0%
ACTACAATGACTGCTTTGGC+TGG - chr7.4:21965918-21965937 MS.gene51889:CDS 45.0%
AGATGTCTATATGTCTCCGC+AGG - chr7.4:21967640-21967659 MS.gene51889:intron 45.0%
AGTTGACCCGAAAGATCCAT+CGG + chr7.4:21968184-21968203 None:intergenic 45.0%
CAAACTTCTAGTAGGTGCAC+AGG - chr7.4:21968210-21968229 MS.gene51889:intron 45.0%
CAATGGTCTCATGACTCAGA+TGG - chr7.4:21967703-21967722 MS.gene51889:intron 45.0%
CAGTGTCTCTCAAAGTTACC+TGG + chr7.4:21968127-21968146 None:intergenic 45.0%
CGAAGAAGGCGAAGTTGAAT+GGG + chr7.4:21967808-21967827 None:intergenic 45.0%
GACAATCAAGTTCCATCTGC+GGG - chr7.4:21967662-21967681 MS.gene51889:intron 45.0%
GATGGAACTTGATTGTCCTG+CGG + chr7.4:21967659-21967678 None:intergenic 45.0%
GATTGTACTGCACTTGGGTA+TGG - chr7.4:21968331-21968350 MS.gene51889:CDS 45.0%
GCATTCTTGTCACCTTCTGT+TGG + chr7.4:21967968-21967987 None:intergenic 45.0%
GGACAATCAAGTTCCATCTG+CGG - chr7.4:21967661-21967680 MS.gene51889:intron 45.0%
GGGTTAAACATGCACCACAT+TGG + chr7.4:21968253-21968272 None:intergenic 45.0%
GGTGACAAGAATGCCAATTC+CGG - chr7.4:21967974-21967993 MS.gene51889:intron 45.0%
GTTCCCGTAGAGACTTAAGA+AGG + chr7.4:21967777-21967796 None:intergenic 45.0%
TCCCGTAGAGACTTAAGAAG+GGG + chr7.4:21967775-21967794 None:intergenic 45.0%
TCGAAGAAGGCGAAGTTGAA+TGG + chr7.4:21967809-21967828 None:intergenic 45.0%
TCTGTTTGGACTCATCGATG+AGG - chr7.4:21968084-21968103 MS.gene51889:intron 45.0%
TGTTGTTATGTGGAAGGGCT+TGG - chr7.4:21965789-21965808 MS.gene51889:CDS 45.0%
TTCAACTTCGCCTTCTTCGA+CGG - chr7.4:21967809-21967828 MS.gene51889:intron 45.0%
TTTATCTACCCCGTCGAAGA+AGG + chr7.4:21967822-21967841 None:intergenic 45.0%
! CAGGTAACTTTGAGAGACAC+TGG - chr7.4:21968125-21968144 MS.gene51889:intron 45.0%
! GCTCTCAAATCGATCGGTTT+CGG - chr7.4:21967908-21967927 MS.gene51889:intron 45.0%
! GGTAACTTTGAGAGACACTG+GGG - chr7.4:21968127-21968146 MS.gene51889:intron 45.0%
!!! AGTAACTTTTTTCGGTGCCG+AGG - chr7.4:21966630-21966649 MS.gene51889:CDS 45.0%
!!! GCGAGTTTTGGGATCAAAGA+AGG - chr7.4:21966474-21966493 MS.gene51889:CDS 45.0%
!!! GGAGGTGATTGCTGTTTTAG+GGG - chr7.4:21966691-21966710 MS.gene51889:CDS 45.0%
AAACTTCGATGAATCCGGGG+CGG + chr7.4:21968063-21968082 None:intergenic 50.0%
ATGACTGCTTTGGCTGGTAG+TGG - chr7.4:21965924-21965943 MS.gene51889:CDS 50.0%
GAAGTAGGATGGCCAACAGA+AGG - chr7.4:21967953-21967972 MS.gene51889:intron 50.0%
GTACTACCAGACGCAGAATC+AGG - chr7.4:21968408-21968427 MS.gene51889:CDS 50.0%
TCAACTTCGCCTTCTTCGAC+GGG - chr7.4:21967810-21967829 MS.gene51889:intron 50.0%
TCCCAACCGCTACATATCTG+CGG + chr7.4:21967490-21967509 None:intergenic 50.0%
TCCGCAGATATGTAGCGGTT+GGG - chr7.4:21967486-21967505 MS.gene51889:intron 50.0%
TCTAGACTCGCTGCGAACAA+AGG - chr7.4:21968028-21968047 MS.gene51889:intron 50.0%
TGGACAACCGAAGTTTCCGA+TGG - chr7.4:21968165-21968184 MS.gene51889:intron 50.0%
TGTCAAAAGCGGCCATAGCA+GGG + chr7.4:21967076-21967095 None:intergenic 50.0%
TGTTCGCAGCGAGTCTAGAA+AGG + chr7.4:21968027-21968046 None:intergenic 50.0%
TTCCGCAGATATGTAGCGGT+TGG - chr7.4:21967485-21967504 MS.gene51889:intron 50.0%
TTGTCAAAAGCGGCCATAGC+AGG + chr7.4:21967077-21967096 None:intergenic 50.0%
! GCCATCAATGAAGCTGGTCT+TGG - chr7.4:21967584-21967603 MS.gene51889:intron 50.0%
! TGGTCTTGGTGACACCGTAA+AGG - chr7.4:21967598-21967617 MS.gene51889:intron 50.0%
! TTTCGGCGACACGCCAATTT+TGG - chr7.4:21967925-21967944 MS.gene51889:intron 50.0%
!! GAAGGGCTTGGTGTAAACTG+GGG - chr7.4:21965801-21965820 MS.gene51889:CDS 50.0%
!! GGAAGGGCTTGGTGTAAACT+GGG - chr7.4:21965800-21965819 MS.gene51889:CDS 50.0%
!! TGGAAGGGCTTGGTGTAAAC+TGG - chr7.4:21965799-21965818 MS.gene51889:CDS 50.0%
CAACTTCGCCTTCTTCGACG+GGG - chr7.4:21967811-21967830 MS.gene51889:intron 55.0%
GACGATCCTGATTCTGCGTC+TGG + chr7.4:21968417-21968436 None:intergenic 55.0%
GGCCGAAATATTCCCGCAGA+TGG + chr7.4:21967677-21967696 None:intergenic 55.0%
TCGCAGCGAGTCTAGAAAGG+AGG + chr7.4:21968024-21968043 None:intergenic 55.0%
!! GGCGACACGCCAATTTTGGT+AGG - chr7.4:21967929-21967948 MS.gene51889:intron 55.0%
!! GACGCGAAGAGCATTGCTCC+AGG - chr7.4:21968106-21968125 MS.gene51889:intron 60.0%
AAAGGAACCCCTCTCCGCCC+CGG - chr7.4:21968046-21968065 MS.gene51889:intron 65.0%
CGATGAATCCGGGGCGGAGA+GGG + chr7.4:21968057-21968076 None:intergenic 65.0%
GATGAATCCGGGGCGGAGAG+GGG + chr7.4:21968056-21968075 None:intergenic 65.0%
TCGATGAATCCGGGGCGGAG+AGG + chr7.4:21968058-21968077 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 21965777 21968597 21965777 ID=MS.gene51889
chr7.4 mRNA 21965777 21968597 21965777 ID=MS.gene51889.t1;Parent=MS.gene51889
chr7.4 exon 21968305 21968597 21968305 ID=MS.gene51889.t1.exon1;Parent=MS.gene51889.t1
chr7.4 CDS 21968305 21968597 21968305 ID=cds.MS.gene51889.t1;Parent=MS.gene51889.t1
chr7.4 exon 21965777 21966881 21965777 ID=MS.gene51889.t1.exon2;Parent=MS.gene51889.t1
chr7.4 CDS 21965777 21966881 21965777 ID=cds.MS.gene51889.t1;Parent=MS.gene51889.t1
Gene Sequence

>MS.gene51889

ATGGGTTGTTTTTGTTGTTATGTGGAAGGGCTTGGTGTAAACTGGGGCACACAAGCAACACACAAGTTACCACCAGAAACTGTTGTGAAGATGTTGAAGGATAATGGGATTCAAAAAGTTAAGCTTTTTGATGCAGATGATACTACAATGACTGCTTTGGCTGGTAGTGGAATTGAAGTTATGGTTGCTATTCCTAATAATCAACTTGCTGAAATGAATGATTATAAACGTGCTCAATATTGGGTTAGGAAGAATGTTACTCGTTATAACTTTGATGGCGGTGTTAAGATCAAGTGAGTTTTTTATTTTTATTTTTTATAATTAGTTAATTGATTTGATCTGTTGATGCTATTGATATTGATCTGCATTTGATGTTTAAGTGTTTGATTATTTGTATTGAAATTTTTCGAGATTTTGTGTTGGATTTTTATCACTTTTAATCCTTGTATAATGTGCTTGCTTCATTGGTTTAGTGTCAGTTAATTGATTTTTTTTTTTAATGTTATTATTGAGTATTGATCTGCATTTGATGTTTAAGTCATTGATTGTCTATGTTGATTTTTTTGTTAGATTTTTGATTCATGGTTAGGTCTATTTTTATATTTTTATTTTTGTCAGGGTTTACTTTCATAAATGTTAAATTTTTTAAGCCCTTTGTGTTCATAATAATGATTATGATTATGCATTTTGATTGATGGCGAGTTTTGGGATCAAAGAAGGAAAAGTGTGAAAAGAATTTTTCAAAAGTGAGCTCTGAAGTTGTTCTTGTTTTAATCAAGGAAGCTTTATGTACTGGGTGATTCCCTCAGAATTAGATTCTTTTATTGGTGTTGGTGATTGATTGATTCCTTGTAGTAACTTTTTTCGGTGCCGAGGTATAATGTATTTGTTCTGAGAATCATTTTACCATTCTAGGAGGTGATTGCTGTTTTAGGGGAAGAGAAATGGTATCAGTCTCTATCTAATTTTGTATGTGCATATATCATATTCTTGGAAGGATTTTTTTTTTTTTTTTTTTGCATGTGCGCGTATATGGGAGAAAAATAAGTAATTGATTGAGAGTTCATATAACAGGAAATTTTGCATCAAGGAATAACAATACAATTAAAATTTGTGATGCATTCTTGTCTCTTCAGTTTTCCTTCTGTATTATAGTTGGTTGATGAGAAGTCTGTTCAAACAATTTTAAAGGAGTTAGATTGATGCTTGTAGAGATTTTATTGATGGTGTCTTTTCATAATTTCATCAGTGTTGCCAATTGCTGATTGTGAAAAATAGCAGTTTATTCAATTGTAGCCCTGCTATGGCCGCTTTTGACAACACTAGATTTAATGGGATTTTATTGAACATTTTGAAGGAAAAGCCGCTTCTTATTATTAAACACCACATTTTAAGCTTTGATGAATATATATTGAATATTGATTGTTTCAACTTCTCTTTAAATGCTAATTTTGTTAAGTGTTAATTCTTAAGCAAATTTGTAAATCATATTAGCCAATAATTTGTAATGCTGGTTGCGATGTTTATTGTCGCCTTGTTTTTGTCATTGGATTGGTCAAACACTAAAATGGTGGTATTGTAAAGCTCCCCAAATTCATCAATGCCATCTTTAGTTGGAAGCAGTAGTACTATCAATGAAATGTATAGTTTATTTCCTTGCCGAATTTCGACCGATTTTAATGTGAATTTGTGCATTTTGTCCATCTTTTTCCGCAGATATGTAGCGGTTGGGAATGAGCCATTTTTGAAAGCTTACAATAATTCATTCTTGAACATCACCTTTCCTGCACTGCAGAACATTCAAAATGCCATCAATGAAGCTGGTCTTGGTGACACCGTAAAGGCAACAGTACCCTTAAACGCAGATGTCTATATGTCTCCGCAGGACAATCAAGTTCCATCTGCGGGAATATTTCGGCCCGATATCAATGGTCTCATGACTCAGATGGTACAATTTCTCAACAAGAACGGAGCACCATTTACTGTCAACATTTACCCCTTCTTAAGTCTCTACGGGAACGACGATTTCCCATTCAACTTCGCCTTCTTCGACGGGGTAGATAAACCAATAACTGACAATGGAATCCAATATACCAACGTCTTTGATGCAAATTTCGATACATTGGTTTCTGCTCTCAAATCGATCGGTTTCGGCGACACGCCAATTTTGGTAGGAGAAGTAGGATGGCCAACAGAAGGTGACAAGAATGCCAATTCCGGCAATGCAATAAGATTCTACAATGGCCTCCTTTCTAGACTCGCTGCGAACAAAGGAACCCCTCTCCGCCCCGGATTCATCGAAGTTTATCTGTTTGGACTCATCGATGAGGACGCGAAGAGCATTGCTCCAGGTAACTTTGAGAGACACTGGGGAATATTTCGATATGATGGACAACCGAAGTTTCCGATGGATCTTTCGGGTCAACTTCAAAACAAACTTCTAGTAGGTGCACAGGGTGTGAAATATCTTGATCCAATGTGGTGCATGTTTAACCCTGATGCTAAAGATCTCAGCAAACTTGCTGATAACATTAACTTTGCTTGCACCTTTGGAGATTGTACTGCACTTGGGTATGGATCTTCTTGCAACAACCTTGATGCTAATGGAAATGCTTCTTATGCATTCAACATGTACTACCAGACGCAGAATCAGGATCGTCAAGCATGCAATTTTGAAGGTTTAGCTAAGCCTACTACAGACAACATTTCAACACCTACTTGCAATTTTATCATCCAGATAGTTCCTTCTTCAGCATCTTCTTTGATGCCCTCATTTGCTGCTTTGTTGTTTATTGCTTTTTCTGTGATTCTATTGGCTTAG
Protein sequence

>MS.gene51889.t1

MGCFCCYVEGLGVNWGTQATHKLPPETVVKMLKDNGIQKVKLFDADDTTMTALAGSGIEVMVAIPNNQLAEMNDYKRAQYWVRKNVTRYNFDGGVKIKYVAVGNEPFLKAYNNSFLNITFPALQNIQNAINEAGLGDTVKATVPLNADVYMSPQDNQVPSAGIFRPDINGLMTQMVQFLNKNGAPFTVNIYPFLSLYGNDDFPFNFAFFDGVDKPITDNGIQYTNVFDANFDTLVSALKSIGFGDTPILVGEVGWPTEGDKNANSGNAIRFYNGLLSRLAANKGTPLRPGFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLSGQLQNKLLVGAQGVKYLDPMWCMFNPDAKDLSKLADNINFACTFGDCTALGYGSSCNNLDANGNASYAFNMYYQTQNQDRQACNFEGLAKPTTDNISTPTCNFIIQIVPSSASSLMPSFAALLFIAFSVILLA