AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08651

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08651.t1 MTR_4g058930 98.854 698 7 1 1 697 1 698 0.0 1428
MS.gene08651.t1 MTR_4g058930 98.810 672 7 1 1 671 1 672 0.0 1373
MS.gene08651.t1 MTR_2g094570 71.034 725 156 20 1 697 57 755 0.0 979
MS.gene08651.t1 MTR_2g094570 71.034 725 156 20 1 697 1 699 0.0 978
MS.gene08651.t1 MTR_2g094570 70.667 675 152 19 1 651 1 653 0.0 909
MS.gene08651.t1 MTR_1g064430 58.874 693 219 14 9 664 37 700 0.0 764
MS.gene08651.t1 MTR_1g064430 58.874 693 219 14 9 664 24 687 0.0 763
MS.gene08651.t1 MTR_1g094960 58.957 575 204 14 1 554 8 571 0.0 596
MS.gene08651.t1 MTR_7g101280 55.453 541 214 13 9 536 28 554 0.0 570
MS.gene08651.t1 MTR_0041s0110 55.453 541 214 13 9 536 28 554 0.0 570
MS.gene08651.t1 MTR_3g073420 41.786 560 295 13 9 558 16 554 3.29e-122 377
MS.gene08651.t1 MTR_7g101275 51.491 369 126 7 21 385 1 320 6.01e-119 362
MS.gene08651.t1 MTR_0041s0120 50.400 375 133 8 21 385 1 332 4.34e-117 357
MS.gene08651.t1 MTR_5g061220 39.920 501 233 16 12 493 19 470 2.36e-97 310
MS.gene08651.t1 MTR_2g024430 40.876 411 158 11 9 402 17 359 9.31e-89 287
MS.gene08651.t1 MTR_5g040880 37.372 487 215 13 4 464 3 425 7.20e-86 280
MS.gene08651.t1 MTR_5g082140 38.174 482 210 14 1 463 1 413 4.07e-84 273
MS.gene08651.t1 MTR_2g018690 38.926 447 205 10 2 436 13 403 4.72e-83 281
MS.gene08651.t1 MTR_8g079492 39.013 446 205 10 2 436 13 402 1.53e-82 280
MS.gene08651.t1 MTR_8g079492 38.702 447 206 10 2 436 13 403 1.06e-81 278
MS.gene08651.t1 MTR_5g076270 39.186 393 186 7 5 388 17 365 2.21e-79 270
MS.gene08651.t1 MTR_2g006270 35.463 454 211 11 3 447 4 384 5.11e-77 254
MS.gene08651.t1 MTR_8g100050 38.847 399 185 7 12 399 59 409 6.94e-77 261
MS.gene08651.t1 MTR_4g088210 40.104 384 171 9 12 381 38 376 8.32e-77 260
MS.gene08651.t1 MTR_4g088210 40.104 384 171 9 12 381 38 376 1.02e-76 260
MS.gene08651.t1 MTR_8g100050 38.847 399 185 7 12 399 59 409 1.84e-76 262
MS.gene08651.t1 MTR_3g064050 37.740 416 204 8 6 412 20 389 4.64e-76 261
MS.gene08651.t1 MTR_1g024025 40.415 386 174 8 6 381 37 376 5.47e-76 266
MS.gene08651.t1 MTR_2g093740 38.278 418 192 9 12 410 56 426 4.31e-74 253
MS.gene08651.t1 MTR_2g093740 38.278 418 192 9 12 410 56 426 1.41e-73 253
MS.gene08651.t1 MTR_2g093740 38.278 418 192 9 12 410 56 426 3.52e-73 253
MS.gene08651.t1 MTR_1g058210 36.946 406 186 10 9 401 9 357 6.71e-73 243
MS.gene08651.t1 MTR_8g027440 30.769 715 364 22 3 670 16 646 8.27e-73 249
MS.gene08651.t1 MTR_4g124900 35.354 495 249 14 3 484 14 450 2.50e-72 254
MS.gene08651.t1 MTR_4g124900 35.354 495 249 14 3 484 14 450 4.69e-72 254
MS.gene08651.t1 MTR_5g040740 37.563 394 170 8 9 391 9 337 8.08e-72 239
MS.gene08651.t1 MTR_2g043250 38.010 392 184 7 3 383 14 357 2.96e-71 251
MS.gene08651.t1 MTR_4g060460 37.956 411 187 6 12 402 54 416 5.50e-71 246
MS.gene08651.t1 MTR_4g060460 37.956 411 187 6 12 402 54 416 6.06e-71 246
MS.gene08651.t1 MTR_2g093740 37.123 431 192 10 12 410 56 439 1.71e-70 243
MS.gene08651.t1 MTR_2g005240 36.989 465 203 14 12 440 23 433 2.59e-70 242
MS.gene08651.t1 MTR_2g093740 37.123 431 192 10 12 410 56 439 1.09e-69 242
MS.gene08651.t1 MTR_2g093740 37.123 431 192 10 12 410 56 439 1.46e-69 243
MS.gene08651.t1 MTR_7g062540 37.028 424 199 14 1 409 3 373 2.87e-67 234
MS.gene08651.t1 MTR_8g101360 36.735 392 195 7 2 384 21 368 2.95e-63 228
MS.gene08651.t1 MTR_8g079492 37.534 373 175 10 67 436 3 320 1.13e-57 210
MS.gene08651.t1 MTR_2g014770 48.826 213 100 2 6 209 47 259 1.18e-57 206
MS.gene08651.t1 MTR_2g014770 37.963 108 66 1 274 381 281 387 2.56e-18 89.7
MS.gene08651.t1 MTR_2g006380 31.799 478 223 17 7 467 15 406 2.78e-57 201
MS.gene08651.t1 MTR_2g014770 48.826 213 100 2 6 209 47 259 4.05e-57 206
MS.gene08651.t1 MTR_2g014770 31.183 186 114 5 274 446 281 465 1.49e-18 90.5
MS.gene08651.t1 MTR_5g061890 36.683 398 167 15 9 387 9 340 2.68e-56 197
MS.gene08651.t1 MTR_5g060780 35.969 392 169 14 9 382 9 336 1.02e-55 196
MS.gene08651.t1 MTR_2g093740 40.373 322 142 7 91 410 23 296 6.72e-55 199
MS.gene08651.t1 MTR_5g460920 35.369 393 170 14 9 382 9 336 8.79e-55 193
MS.gene08651.t1 MTR_2g093740 40.373 322 142 7 91 410 23 296 1.02e-54 199
MS.gene08651.t1 MTR_4g021580 49.528 212 99 4 7 211 14 224 3.13e-53 194
MS.gene08651.t1 MTR_4g021580 49.528 212 99 4 7 211 14 224 4.94e-53 194
MS.gene08651.t1 MTR_2g093740 38.806 335 142 8 91 410 23 309 2.32e-51 189
MS.gene08651.t1 MTR_2g093740 37.910 335 145 7 91 410 23 309 3.08e-51 189
MS.gene08651.t1 MTR_8g446900 31.608 367 191 8 56 422 12 318 3.83e-50 180
MS.gene08651.t1 MTR_5g060630 31.628 430 202 14 9 398 9 386 3.60e-48 178
MS.gene08651.t1 MTR_5g460740 31.628 430 202 14 9 398 9 386 3.60e-48 178
MS.gene08651.t1 MTR_5g062970 43.367 196 93 3 12 196 38 226 6.59e-47 166
MS.gene08651.t1 MTR_5g062960 37.569 181 85 5 275 451 1 157 9.84e-25 102
MS.gene08651.t1 MTR_3g064050 39.231 130 75 3 284 412 26 152 4.36e-17 85.9
MS.gene08651.t1 MTR_5g032260 39.048 105 62 2 275 378 49 152 8.94e-14 74.3
MS.gene08651.t1 MTR_5g060770 29.670 182 108 6 55 227 6 176 1.43e-13 73.2
MS.gene08651.t1 MTR_1g058350 32.432 148 83 4 103 238 71 213 4.76e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08651.t1 AT2G28350 58.108 740 217 21 3 697 2 693 0.0 753
MS.gene08651.t1 AT4G30080 55.925 692 244 15 4 669 12 668 0.0 717
MS.gene08651.t1 AT2G28350 57.644 713 209 21 3 670 2 666 0.0 714
MS.gene08651.t1 AT1G77850 43.450 458 221 11 9 451 16 450 1.31e-115 360
MS.gene08651.t1 AT1G77850 39.161 572 294 16 9 559 16 554 1.31e-115 360
MS.gene08651.t1 AT1G30330 40.097 414 193 8 3 407 14 381 6.18e-81 276
MS.gene08651.t1 AT1G30330 40.049 412 192 8 5 407 18 383 2.46e-80 275
MS.gene08651.t1 AT1G30330 40.049 412 192 8 5 407 18 383 2.46e-80 275
MS.gene08651.t1 AT5G37020 38.817 389 185 7 9 388 19 363 3.43e-79 268
MS.gene08651.t1 AT5G37020 38.817 389 185 7 9 388 19 363 1.15e-78 268
MS.gene08651.t1 AT1G59750 31.241 685 351 19 12 662 22 620 5.25e-78 263
MS.gene08651.t1 AT2G33860 38.259 379 181 6 12 381 54 388 9.00e-76 255
MS.gene08651.t1 AT5G60450 38.670 406 184 9 12 400 41 398 1.90e-75 258
MS.gene08651.t1 AT5G60450 38.670 406 184 9 12 400 65 422 3.28e-75 258
MS.gene08651.t1 AT5G62000 39.409 406 185 10 9 400 52 410 6.01e-74 256
MS.gene08651.t1 AT5G62000 39.409 406 185 10 9 400 52 410 6.01e-74 256
MS.gene08651.t1 AT5G62000 39.409 406 185 10 9 400 52 410 6.01e-74 256
MS.gene08651.t1 AT5G62000 39.409 406 185 10 9 400 52 410 6.01e-74 256
MS.gene08651.t1 AT5G62000 39.409 406 185 10 9 400 52 410 7.99e-74 255
MS.gene08651.t1 AT1G59750 30.904 686 350 22 12 662 22 618 2.39e-71 245
MS.gene08651.t1 AT1G59750 31.050 686 354 22 12 662 22 623 7.87e-71 243
MS.gene08651.t1 AT1G59750 31.050 686 354 22 12 662 22 623 7.87e-71 243
MS.gene08651.t1 AT2G46530 39.005 382 169 12 12 381 21 350 4.51e-67 232
MS.gene08651.t1 AT2G46530 39.005 382 169 12 12 381 42 371 9.75e-67 231
MS.gene08651.t1 AT1G19850 37.755 392 181 9 4 381 46 388 2.54e-66 235
MS.gene08651.t1 AT1G19850 37.755 392 181 9 4 381 35 377 3.03e-66 235
MS.gene08651.t1 AT3G61830 36.131 429 206 13 4 418 14 388 3.07e-65 227
MS.gene08651.t1 AT1G35520 29.750 679 343 24 6 660 21 589 5.70e-61 215
MS.gene08651.t1 AT1G34390 28.152 682 362 22 6 667 21 594 6.51e-59 209
MS.gene08651.t1 AT4G23980 35.279 394 176 12 8 381 8 342 5.06e-58 207
MS.gene08651.t1 AT4G23980 35.279 394 176 12 8 381 8 342 5.07e-58 208
MS.gene08651.t1 AT1G35240 36.364 396 183 14 6 387 21 361 1.33e-57 206
MS.gene08651.t1 AT1G34310 35.294 408 197 13 6 402 21 372 1.63e-57 205
MS.gene08651.t1 AT1G19220 48.869 221 96 4 3 211 14 229 2.63e-57 211
MS.gene08651.t1 AT1G19220 40.000 130 67 4 286 412 261 382 2.24e-17 87.4
MS.gene08651.t1 AT5G37020 38.699 292 134 5 98 388 21 268 9.03e-57 206
MS.gene08651.t1 AT1G35540 34.663 401 188 13 6 395 21 358 1.84e-56 203
MS.gene08651.t1 AT1G34410 35.401 387 184 12 6 381 21 352 2.65e-56 202
MS.gene08651.t1 AT5G20730 47.059 221 100 4 3 211 15 230 1.03e-55 206
MS.gene08651.t1 AT5G20730 42.105 114 64 2 286 398 262 374 5.86e-17 85.9
MS.gene08651.t1 AT5G20730 47.059 221 100 4 3 211 15 230 1.11e-55 206
MS.gene08651.t1 AT5G20730 42.478 113 64 1 286 398 262 373 2.96e-18 90.1
MS.gene08651.t1 AT5G20730 47.059 221 100 4 3 211 15 230 1.13e-55 206
MS.gene08651.t1 AT5G20730 42.105 114 64 2 286 398 262 374 4.63e-17 86.3
MS.gene08651.t1 AT2G33860 39.636 275 120 5 108 381 2 231 4.66e-54 192
MS.gene08651.t1 AT3G61830 35.946 370 180 10 52 418 22 337 1.44e-52 191
MS.gene08651.t1 AT1G34170 33.075 387 195 12 6 381 19 352 4.36e-52 188
MS.gene08651.t1 AT2G46530 38.406 276 121 6 107 381 36 263 3.71e-46 172
MS.gene08651.t1 AT1G34170 38.636 220 118 5 6 214 19 232 7.35e-42 159
MS.gene08651.t1 AT1G34170 38.636 220 118 5 6 214 19 232 2.73e-41 159
MS.gene08651.t1 AT1G43950 39.336 211 110 6 6 207 21 222 9.15e-38 140
MS.gene08651.t1 AT2G36080 37.838 111 63 3 103 208 33 142 1.76e-11 63.9
MS.gene08651.t1 AT2G36080 37.838 111 63 3 103 208 33 142 1.76e-11 63.9
MS.gene08651.t1 AT2G36080 37.838 111 63 3 103 208 33 142 3.80e-11 64.3
MS.gene08651.t1 AT2G36080 37.838 111 63 3 103 208 33 142 3.99e-11 64.3

Find 162 sgRNAs with CRISPR-Local

Find 245 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTAGGATTAGTTGGTTTA+TGG 0.143329 4.1:-39400963 MS.gene08651:CDS
TTGAAACTGAAGACTCTTCT+AGG 0.222931 4.1:-39400979 MS.gene08651:CDS
GGAAAGTGAGAAAGGGTATT+TGG 0.265159 4.1:-39401947 MS.gene08651:CDS
TTGACAATTCTGACAGATTC+AGG 0.268164 4.1:-39399034 MS.gene08651:CDS
ACCCTTGACTTATCACTTCT+TGG 0.269886 4.1:-39399541 MS.gene08651:CDS
TTCTCCGTGGCGCCTTCTTC+AGG 0.276881 4.1:-39400894 MS.gene08651:intron
TCATCTTGTGGATGGACTTC+AGG 0.278431 4.1:-39401295 MS.gene08651:CDS
CTAATACAAACATTGATCTA+AGG 0.281084 4.1:-39401828 MS.gene08651:CDS
AACAATGTAAATTGTGGAAT+TGG 0.287100 4.1:-39401262 MS.gene08651:CDS
CAGGCATGATAAGCTTTCTT+TGG 0.290621 4.1:+39399852 None:intergenic
TGGAATTCGTAGGGCGAAAA+AGG 0.294025 4.1:-39401341 MS.gene08651:CDS
GGGCCAAACAGCCCCTTCTT+TGG 0.299415 4.1:-39400063 MS.gene08651:CDS
GGAGCCAGGCATGCTCAATT+TGG 0.301173 4.1:-39400009 MS.gene08651:CDS
TGTGGATGGACTTCAGGTTC+TGG 0.309717 4.1:-39401289 MS.gene08651:CDS
AGAAGGCGCCACGGAGAATT+AGG 0.313389 4.1:+39400899 None:intergenic
GGGAACAATTGCTTCTGTTC+AGG 0.316761 4.1:-39400942 MS.gene08651:CDS
CACCAGCAACAAGCTTCTTC+TGG 0.317024 4.1:+39401407 None:intergenic
ATGAGGATTCAATGGTGTTC+TGG 0.321716 4.1:-39401022 MS.gene08651:CDS
ACTGAAGTTTATTGTTGTTA+AGG 0.329691 4.1:+39399962 None:intergenic
TCTAGGATTAGTTGGTTTAT+GGG 0.340586 4.1:-39400962 MS.gene08651:CDS
CGCCGGCACTTGCTTACAAC+AGG 0.344728 4.1:-39401448 MS.gene08651:CDS
AATTGTGGAATTGGACCTTT+TGG 0.346313 4.1:-39401253 MS.gene08651:CDS
TGGCAACTTGGATTGGATAT+TGG 0.355146 4.1:-39399604 MS.gene08651:CDS
TAATGAAGCTTCTTCCTTTG+AGG 0.360496 4.1:-39401723 MS.gene08651:CDS
AACAACAATAAACTTCAGTT+AGG 0.369261 4.1:-39399958 MS.gene08651:CDS
TTCACCACTGATTCTTTGTA+AGG 0.370999 4.1:-39401797 MS.gene08651:CDS
TTCTTCCTTGGTGGAGTGAA+AGG 0.377163 4.1:+39400162 None:intergenic
GGTTGTTGGTGTTAAATTCA+TGG 0.380615 4.1:-39401776 MS.gene08651:CDS
TGTTATGGAAGCTGTGAAAT+TGG 0.385027 4.1:-39401134 MS.gene08651:CDS
AGTGAGGGCTGAAGATGTTA+TGG 0.385830 4.1:-39401149 MS.gene08651:CDS
AAACCAAAGAAGGGGCTGTT+TGG 0.394320 4.1:+39400060 None:intergenic
AGACAAAGAGAAGTGGTTCT+TGG 0.394810 4.1:-39399671 MS.gene08651:CDS
CAATGCTCCTGCATGTATAC+AGG 0.395010 4.1:-39400031 MS.gene08651:CDS
AGGATGTGCACGGTGAGCTT+TGG 0.396378 4.1:-39401501 MS.gene08651:CDS
AAGACTCTTCTAGGATTAGT+TGG 0.399400 4.1:-39400970 MS.gene08651:CDS
ACAGGTGCTGTTAAGCAAAT+TGG 0.408215 4.1:-39399430 MS.gene08651:CDS
GAGAATAAGACATTGAGAAA+TGG 0.411485 4.1:-39401205 MS.gene08651:CDS
CACTCCCGAGTTCTGTGTTA+AGG 0.419777 4.1:-39401065 MS.gene08651:CDS
AGGTGGTGGTGGTGATGGTT+TGG 0.423416 4.1:-39401677 MS.gene08651:CDS
TTCGCCACCACCGTCTGAAC+AGG 0.425252 4.1:+39401523 None:intergenic
CTGATTCTTTGTAAGGTTGT+TGG 0.426252 4.1:-39401790 MS.gene08651:CDS
CTAATGCTGCTGAGTTTAGT+TGG 0.428567 4.1:-39399624 MS.gene08651:CDS
GCCTACTACTACAGGTTACA+TGG 0.430758 4.1:-39400272 MS.gene08651:intron
GGATAGTAAACAACTTCAAA+TGG 0.432142 4.1:+39401097 None:intergenic
AGCACCTTGATGTGCATACT+AGG 0.434168 4.1:-39399915 MS.gene08651:CDS
ACGGTGAGCTTTGGAAGTTT+AGG 0.437969 4.1:-39401492 MS.gene08651:CDS
TGAGGATTCAATGGTGTTCT+GGG 0.446254 4.1:-39401021 MS.gene08651:CDS
TTCAATGGTGTTCTGGGATG+AGG 0.448575 4.1:-39401015 MS.gene08651:CDS
TCCTCCAAATTGAGCATGCC+TGG 0.451469 4.1:+39400005 None:intergenic
GATGAAGGTGGTGGTGGTGA+TGG 0.454860 4.1:-39401682 MS.gene08651:CDS
GGAAACCGCAGCTTCTTCCT+TGG 0.455567 4.1:+39400150 None:intergenic
CATTATGCAAATATGCTTAG+AGG 0.456170 4.1:+39398830 None:intergenic
CAGCTATGGCATGCTTGTGC+TGG 0.456859 4.1:-39401919 MS.gene08651:CDS
AACCGCAGCTTCTTCCTTGG+TGG 0.459037 4.1:+39400153 None:intergenic
TATCATGCCTGTTGAGTATC+GGG 0.463632 4.1:-39399840 MS.gene08651:CDS
TCAAGGTGCTGAATGTTAGT+AGG 0.470920 4.1:+39399928 None:intergenic
AAGTTTAGGCATATTTATAG+AGG 0.471597 4.1:-39401478 MS.gene08651:CDS
AACCAGAAGAAGCTTGTTGC+TGG 0.473424 4.1:-39401409 MS.gene08651:CDS
GCTGAGTTTAGTTGGCAACT+TGG 0.473727 4.1:-39399616 MS.gene08651:CDS
AACTCGGGAGTGCTAGCTCT+TGG 0.477183 4.1:+39401076 None:intergenic
TCTTCCTTGGTGGAGTGAAA+GGG 0.480887 4.1:+39400163 None:intergenic
TCAGAGCTTGGTTATGATGA+AGG 0.480927 4.1:-39401697 MS.gene08651:CDS
TTGGATGCATCAAACAAAAC+AGG 0.481900 4.1:-39398854 MS.gene08651:CDS
TCAATGAAGGAAAGTGAGAA+AGG 0.482138 4.1:-39401955 MS.gene08651:CDS
ATTGTCAGATAAACCAAAGA+AGG 0.484820 4.1:+39400050 None:intergenic
GGTTATGATGAAGGTGGTGG+TGG 0.486406 4.1:-39401688 MS.gene08651:CDS
TGAGTGGAAGAGTGAGAGTG+AGG 0.488015 4.1:-39401165 MS.gene08651:CDS
TCACAAAGGTGAACATGGAT+TGG 0.488178 4.1:-39398873 MS.gene08651:CDS
GTTTAGTTGGCAACTTGGAT+TGG 0.492268 4.1:-39399611 MS.gene08651:CDS
AGGGGCTGTTTGGCCCGAGC+TGG 0.496697 4.1:+39400070 None:intergenic
GGTTCTAACAATGTAAATTG+TGG 0.497055 4.1:-39401268 MS.gene08651:CDS
TTATCATGCCTGTTGAGTAT+CGG 0.500678 4.1:-39399841 MS.gene08651:CDS
TGTTCAGACGGTGGTGGCGA+AGG 0.501569 4.1:-39401521 MS.gene08651:CDS
AGAAGAGTCTTCAGTTTCAA+AGG 0.502379 4.1:+39400982 None:intergenic
ACAACAATAAACTTCAGTTA+GGG 0.502883 4.1:-39399957 MS.gene08651:CDS
GGAGAATTAGGCCAGCGAAT+AGG 0.504468 4.1:+39400911 None:intergenic
TTGATGGTTCATCTTGTGGA+TGG 0.504684 4.1:-39401303 MS.gene08651:CDS
AGGTTGTTGATCCTATTCGC+TGG 0.509848 4.1:-39400922 MS.gene08651:CDS
AATCTGATGCAAACAATGGT+GGG 0.510020 4.1:-39401612 MS.gene08651:CDS
CTATGGCATGCTTGTGCTGG+TGG 0.512929 4.1:-39401916 MS.gene08651:CDS
GCATGCTTGTGCTGGTGGAA+TGG 0.513876 4.1:-39401911 MS.gene08651:CDS
AGTAAACAACTTCAAATGGT+TGG 0.519885 4.1:+39401101 None:intergenic
TTGTGGAAAGTAGAAAACTT+TGG 0.520773 4.1:+39401867 None:intergenic
TGTTAAGGCTTCAGCTGTGA+AGG 0.522163 4.1:-39401050 MS.gene08651:CDS
CCTTTGAGGAATTCAGAGCT+TGG 0.522861 4.1:-39401709 MS.gene08651:CDS
GGCTGAGCCGCCTGTTCAGA+CGG 0.523312 4.1:-39401533 MS.gene08651:CDS
CTGCATGTATACAGGGAGCC+AGG 0.523472 4.1:-39400023 MS.gene08651:CDS
GAAGAGTCTTCAGTTTCAAA+GGG 0.524779 4.1:+39400983 None:intergenic
ATTTGTTGCTCAGTGAGTAT+TGG 0.526915 4.1:+39399736 None:intergenic
TAATACAAACATTGATCTAA+GGG 0.528805 4.1:-39401827 MS.gene08651:CDS
CTTTCCAAGGCTTGATTACG+CGG 0.534891 4.1:-39401554 MS.gene08651:CDS
TGAAGCCTTAACACAGAACT+CGG 0.535150 4.1:+39401060 None:intergenic
AAATTGGTGAAGAACCCTTC+AGG 0.536171 4.1:-39399414 MS.gene08651:intron
CAATGAAGGAAAGTGAGAAA+GGG 0.542351 4.1:-39401954 MS.gene08651:CDS
AACCAAGAAGTGATAAGTCA+AGG 0.543232 4.1:+39399539 None:intergenic
ACCCTGTTGTAAGCAAGTGC+CGG 0.547262 4.1:+39401446 None:intergenic
GCATGTTCAGCATGTCCTTG+TGG 0.548555 4.1:+39401850 None:intergenic
CAGCTGTGAAGGCTGCTATG+AGG 0.549739 4.1:-39401039 MS.gene08651:CDS
CTGGCTCCCTGTATACATGC+AGG 0.552767 4.1:+39400024 None:intergenic
GATACTAATTCGACCAACCA+CGG 0.553761 4.1:+39400210 None:intergenic
AATTGTGTGTTGGAATTCGT+AGG 0.556594 4.1:-39401351 MS.gene08651:CDS
ATCGTCTCAGCACAGTACCT+TGG 0.557673 4.1:+39401577 None:intergenic
TCTGATGCAAACAATGGTGG+GGG 0.561128 4.1:-39401610 MS.gene08651:CDS
GCCGCCTGTTCAGACGGTGG+TGG 0.561486 4.1:-39401527 MS.gene08651:CDS
ATACTAATTCGACCAACCAC+GGG 0.564828 4.1:+39400211 None:intergenic
GCCAGGCATGCTCAATTTGG+AGG 0.565841 4.1:-39400006 MS.gene08651:CDS
ATTGGATATTGGTCATTGCA+AGG 0.566046 4.1:-39399593 MS.gene08651:CDS
AAATCCTAGTATGCACATCA+AGG 0.567436 4.1:+39399911 None:intergenic
GGAACTCACAAAGGTGAACA+TGG 0.567844 4.1:-39398878 MS.gene08651:CDS
GAAGCGTGTTAGCCCGTGGT+TGG 0.567936 4.1:-39400223 MS.gene08651:CDS
TTGTCAGATAAACCAAAGAA+GGG 0.569032 4.1:+39400051 None:intergenic
CAATCTGATGCAAACAATGG+TGG 0.570087 4.1:-39401613 MS.gene08651:CDS
AGTTTAGGCATATTTATAGA+GGG 0.570327 4.1:-39401477 MS.gene08651:CDS
GCTTGTTGCTGGTGATTCTG+TGG 0.572232 4.1:-39401398 MS.gene08651:CDS
ACCAAGAAGTGATAAGTCAA+GGG 0.572563 4.1:+39399540 None:intergenic
AAAACTTTGGAGTTCACTTG+AGG 0.572950 4.1:+39401880 None:intergenic
ATCTGATGCAAACAATGGTG+GGG 0.580572 4.1:-39401611 MS.gene08651:CDS
AAAGTCTTAGCAAAAGATGC+AGG 0.580849 4.1:+39401640 None:intergenic
CCAAGCTCTGAATTCCTCAA+AGG 0.581047 4.1:+39401709 None:intergenic
GAGAATGGTGAATTGTGTGT+TGG 0.591957 4.1:-39401361 MS.gene08651:CDS
GTGCTGAGACGATCTTTCCA+AGG 0.594168 4.1:-39401567 MS.gene08651:CDS
GAGTGGAAGAGTGAGAGTGA+GGG 0.596583 4.1:-39401164 MS.gene08651:CDS
TTTATAGAGGGACACCGCGC+CGG 0.597722 4.1:-39401465 MS.gene08651:CDS
TTGCCTGAAAACGTCGGTGA+CGG 0.603464 4.1:+39400093 None:intergenic
TGGAGTCAGAAGATGTTGGA+AGG 0.603517 4.1:-39399564 MS.gene08651:CDS
AGGCTGCTATGAGGATTCAA+TGG 0.603915 4.1:-39401030 MS.gene08651:CDS
CTCCACCAAGGAAGAAGCTG+CGG 0.604549 4.1:-39400155 MS.gene08651:CDS
GGTATTTGGATCCACAGCTA+TGG 0.606132 4.1:-39401933 MS.gene08651:CDS
ATAGTAAAGACAAAGAGAAG+TGG 0.610021 4.1:-39399678 MS.gene08651:CDS
ACACAAAAGCAATACCTGAA+GGG 0.611431 4.1:+39399400 None:intergenic
GCCACCACCGTCTGAACAGG+CGG 0.614156 4.1:+39401526 None:intergenic
TTCTTGGAGTCAGAAGATGT+TGG 0.615581 4.1:-39399568 MS.gene08651:CDS
AAAGGGCTGAAATGAATGAC+TGG 0.619094 4.1:+39400180 None:intergenic
CCTACTACTACAGGTTACAT+GGG 0.623681 4.1:-39400271 MS.gene08651:intron
TGACGGAAACTGAACATCGA+GGG 0.624449 4.1:+39400110 None:intergenic
CTTGGTTATGATGAAGGTGG+TGG 0.625908 4.1:-39401691 MS.gene08651:CDS
TCAGCCGCGTAATCAAGCCT+TGG 0.630202 4.1:+39401550 None:intergenic
GCACAAGCATGCCATAGCTG+TGG 0.631502 4.1:+39401922 None:intergenic
ATTGTGTGTTGGAATTCGTA+GGG 0.641411 4.1:-39401350 MS.gene08651:CDS
GACGGAAACTGAACATCGAG+GGG 0.641637 4.1:+39400111 None:intergenic
AATGCTCCTGCATGTATACA+GGG 0.643769 4.1:-39400030 MS.gene08651:CDS
GGCTGACCCTGAAACTGATG+AGG 0.645568 4.1:-39401755 MS.gene08651:CDS
ACGTGAAGCGTGTTAGCCCG+TGG 0.646883 4.1:-39400227 MS.gene08651:CDS
AATACCTGAAGAAGGCGCCA+CGG 0.652216 4.1:+39400890 None:intergenic
TAACAGCACCTGTTGCATCA+CGG 0.662294 4.1:+39399440 None:intergenic
GTTGTAAGCAAGTGCCGGCG+CGG 0.664793 4.1:+39401451 None:intergenic
TGAGCCGCCTGTTCAGACGG+TGG 0.665475 4.1:-39401530 MS.gene08651:CDS
GGCACTTGCTTACAACAGGG+TGG 0.673448 4.1:-39401444 MS.gene08651:CDS
GAAGCCTTAACACAGAACTC+GGG 0.673594 4.1:+39401061 None:intergenic
TGGATAACAGGAACTCACAA+AGG 0.673838 4.1:-39398887 MS.gene08651:CDS
TGTCAGATAAACCAAAGAAG+GGG 0.674937 4.1:+39400052 None:intergenic
AGCTGGTTGCCTGAAAACGT+CGG 0.679058 4.1:+39400087 None:intergenic
ACAACCTTACAAAGAATCAG+TGG 0.680398 4.1:+39401793 None:intergenic
TTCGCTGGCCTAATTCTCCG+TGG 0.685289 4.1:-39400907 MS.gene08651:CDS
CTGATGCAAACAATGGTGGG+GGG 0.689649 4.1:-39401609 MS.gene08651:CDS
GCCGGCACTTGCTTACAACA+GGG 0.691948 4.1:-39401447 MS.gene08651:CDS
TCAGCCCTTTCACTCCACCA+AGG 0.700439 4.1:-39400167 MS.gene08651:CDS
GAGCTTGGTTATGATGAAGG+TGG 0.703479 4.1:-39401694 MS.gene08651:CDS
ACTCAATCTGATGCAAACAA+TGG 0.706199 4.1:-39401616 MS.gene08651:CDS
GTGACGGAAACTGAACATCG+AGG 0.721853 4.1:+39400109 None:intergenic
GTGGTGGCGAAGGATGTGCA+CGG 0.739739 4.1:-39401511 MS.gene08651:CDS
ACGGAAACTGAACATCGAGG+GGG 0.755057 4.1:+39400112 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTGTTTTTTAGTTACAATT+TGG - chr4.1:39399914-39399933 MS.gene08651:CDS 15.0%
!!! TTTGTTATTGTTCATTTTTA+TGG - chr4.1:39400007-39400026 MS.gene08651:CDS 15.0%
!! AATGAAACAATTAGTAGTAT+AGG + chr4.1:39400124-39400143 None:intergenic 20.0%
!! GAAATGTCTTATATAATAAG+AGG - chr4.1:39401852-39401871 MS.gene08651:CDS 20.0%
!! TAATACAAACATTGATCTAA+GGG - chr4.1:39398957-39398976 MS.gene08651:intron 20.0%
! AAAAAAGAAAATGCTCCAAA+AGG + chr4.1:39399549-39399568 None:intergenic 25.0%
! AACAACAATAAACTTCAGTT+AGG - chr4.1:39400826-39400845 MS.gene08651:intron 25.0%
! AACAATGTAAATTGTGGAAT+TGG - chr4.1:39399522-39399541 MS.gene08651:CDS 25.0%
! AAGTTTAGGCATATTTATAG+AGG - chr4.1:39399306-39399325 MS.gene08651:intron 25.0%
! ACAACAATAAACTTCAGTTA+GGG - chr4.1:39400827-39400846 MS.gene08651:intron 25.0%
! AGTTTAGGCATATTTATAGA+GGG - chr4.1:39399307-39399326 MS.gene08651:intron 25.0%
! CTAATACAAACATTGATCTA+AGG - chr4.1:39398956-39398975 MS.gene08651:intron 25.0%
! TAAAAAACAATACCTGAAGA+AGG + chr4.1:39399905-39399924 None:intergenic 25.0%
! TAAGTTTCACAATACATTCT+AGG - chr4.1:39401562-39401581 MS.gene08651:CDS 25.0%
! TTGAACACATCTTTACATAA+GGG - chr4.1:39399947-39399966 MS.gene08651:CDS 25.0%
!! AAAATAGAGTGAAGTCATTT+TGG + chr4.1:39401620-39401639 None:intergenic 25.0%
!! ACTCAATTTTTATGCTTCTT+TGG - chr4.1:39399970-39399989 MS.gene08651:CDS 25.0%
!! ACTGAAGTTTATTGTTGTTA+AGG + chr4.1:39400825-39400844 None:intergenic 25.0%
!! ATTTTGCTACTGTACAATAA+TGG + chr4.1:39401660-39401679 None:intergenic 25.0%
!! TTTTGCTACTGTACAATAAT+GGG + chr4.1:39401659-39401678 None:intergenic 25.0%
!!! AAACATCAGTTTTGTCTTTT+TGG - chr4.1:39401021-39401040 MS.gene08651:CDS 25.0%
!!! TGAACTTTTTTTCAAACTCT+TGG - chr4.1:39400330-39400349 MS.gene08651:intron 25.0%
AGTAAACAACTTCAAATGGT+TGG + chr4.1:39399686-39399705 None:intergenic 30.0%
AGTACTACTCTTGGATTAAT+GGG - chr4.1:39401680-39401699 MS.gene08651:CDS 30.0%
ATAGTAAAGACAAAGAGAAG+TGG - chr4.1:39401106-39401125 MS.gene08651:CDS 30.0%
ATTGTCAGATAAACCAAAGA+AGG + chr4.1:39400737-39400756 None:intergenic 30.0%
GAAACAATTAGTAGTATAGG+AGG + chr4.1:39400121-39400140 None:intergenic 30.0%
GAAATAACCTGTGTTAAGTA+GGG - chr4.1:39400385-39400404 MS.gene08651:intron 30.0%
GAGAATAAGACATTGAGAAA+TGG - chr4.1:39399579-39399598 MS.gene08651:CDS 30.0%
GGATAGTAAACAACTTCAAA+TGG + chr4.1:39399690-39399709 None:intergenic 30.0%
GTTGAACACATCTTTACATA+AGG - chr4.1:39399946-39399965 MS.gene08651:CDS 30.0%
TAGCAAAATAGTACTACTCT+TGG - chr4.1:39401671-39401690 MS.gene08651:CDS 30.0%
TAGTACTACTCTTGGATTAA+TGG - chr4.1:39401679-39401698 MS.gene08651:CDS 30.0%
TGAAATAACCTGTGTTAAGT+AGG - chr4.1:39400384-39400403 MS.gene08651:intron 30.0%
TTGTAGACTAAACTAACAAC+CGG + chr4.1:39401526-39401545 None:intergenic 30.0%
TTGTCAGATAAACCAAAGAA+GGG + chr4.1:39400736-39400755 None:intergenic 30.0%
TTGTGGAAAGTAGAAAACTT+TGG + chr4.1:39398920-39398939 None:intergenic 30.0%
! AAAGTTTTCTACTTTCCACA+AGG - chr4.1:39398919-39398938 MS.gene08651:intron 30.0%
! GGTTCTAACAATGTAAATTG+TGG - chr4.1:39399516-39399535 MS.gene08651:CDS 30.0%
! TTCACGTTTTGTAGTAAATC+AGG + chr4.1:39400544-39400563 None:intergenic 30.0%
! TTTTCACTCTTGTGATTATG+AGG - chr4.1:39400360-39400379 MS.gene08651:intron 30.0%
!! AGGATCTTGATTGTTTTTCA+GGG - chr4.1:39401872-39401891 MS.gene08651:CDS 30.0%
!! TCTAGGATTAGTTGGTTTAT+GGG - chr4.1:39399822-39399841 MS.gene08651:CDS 30.0%
!! TTCATTTTTATGGTCATCTC+TGG - chr4.1:39400017-39400036 MS.gene08651:CDS 30.0%
!! TTCTAGGATTAGTTGGTTTA+TGG - chr4.1:39399821-39399840 MS.gene08651:CDS 30.0%
!!! TGTTTTGATGGTAATTTGAG+TGG - chr4.1:39399603-39399622 MS.gene08651:CDS 30.0%
!!! TTGAGAAATGGTTGTTTTGA+TGG - chr4.1:39399591-39399610 MS.gene08651:CDS 30.0%
AAAGTCTTAGCAAAAGATGC+AGG + chr4.1:39399147-39399166 None:intergenic 35.0%
AAATCCTAGTATGCACATCA+AGG + chr4.1:39400876-39400895 None:intergenic 35.0%
AACACAAAAGCAATACCTGA+AGG + chr4.1:39401388-39401407 None:intergenic 35.0%
AACCAAGAAGTGATAAGTCA+AGG + chr4.1:39401248-39401267 None:intergenic 35.0%
AATCTGATGCAAACAATGGT+GGG - chr4.1:39399172-39399191 MS.gene08651:intron 35.0%
AATTGTGTGTTGGAATTCGT+AGG - chr4.1:39399433-39399452 MS.gene08651:CDS 35.0%
ACAACCTTACAAAGAATCAG+TGG + chr4.1:39398994-39399013 None:intergenic 35.0%
ACACAAAAGCAATACCTGAA+GGG + chr4.1:39401387-39401406 None:intergenic 35.0%
ACCAAGAAGTGATAAGTCAA+GGG + chr4.1:39401247-39401266 None:intergenic 35.0%
ACTCAATCTGATGCAAACAA+TGG - chr4.1:39399168-39399187 MS.gene08651:intron 35.0%
AGAAGAGTCTTCAGTTTCAA+AGG + chr4.1:39399805-39399824 None:intergenic 35.0%
ATTGGATATTGGTCATTGCA+AGG - chr4.1:39401191-39401210 MS.gene08651:CDS 35.0%
ATTGTGTGTTGGAATTCGTA+GGG - chr4.1:39399434-39399453 MS.gene08651:CDS 35.0%
ATTGTTATGCCTACTACTAC+AGG - chr4.1:39400504-39400523 MS.gene08651:intron 35.0%
CAAAAACCTCATCAGTTTCA+GGG + chr4.1:39399038-39399057 None:intergenic 35.0%
CAATCTGGGCAATAATATCA+AGG + chr4.1:39400305-39400324 None:intergenic 35.0%
CTGATTCTTTGTAAGGTTGT+TGG - chr4.1:39398994-39399013 MS.gene08651:intron 35.0%
GAAGAGTCTTCAGTTTCAAA+GGG + chr4.1:39399804-39399823 None:intergenic 35.0%
GTACTACTCTTGGATTAATG+GGG - chr4.1:39401681-39401700 MS.gene08651:CDS 35.0%
GTGATATTGTGGATAATCAC+AGG - chr4.1:39400232-39400251 MS.gene08651:CDS 35.0%
TAATGAAGCTTCTTCCTTTG+AGG - chr4.1:39399061-39399080 MS.gene08651:CDS 35.0%
TACTACTCTTGGATTAATGG+GGG - chr4.1:39401682-39401701 MS.gene08651:CDS 35.0%
TGATATTATTGCCCAGATTG+CGG - chr4.1:39400305-39400324 MS.gene08651:intron 35.0%
TGTCAGATAAACCAAAGAAG+GGG + chr4.1:39400735-39400754 None:intergenic 35.0%
TGTTATGGAAGCTGTGAAAT+TGG - chr4.1:39399650-39399669 MS.gene08651:CDS 35.0%
TTATCATGCCTGTTGAGTAT+CGG - chr4.1:39400943-39400962 MS.gene08651:CDS 35.0%
TTGAAACTGAAGACTCTTCT+AGG - chr4.1:39399805-39399824 MS.gene08651:CDS 35.0%
TTGACAATTCTGACAGATTC+AGG - chr4.1:39401750-39401769 MS.gene08651:CDS 35.0%
TTGGATGCATCAAACAAAAC+AGG - chr4.1:39401930-39401949 MS.gene08651:CDS 35.0%
! AATTGTGGAATTGGACCTTT+TGG - chr4.1:39399531-39399550 MS.gene08651:CDS 35.0%
! ATTTGTTGCTCAGTGAGTAT+TGG + chr4.1:39401051-39401070 None:intergenic 35.0%
! GTCGATTTAGCTGAAATCAT+AGG - chr4.1:39400078-39400097 MS.gene08651:CDS 35.0%
!! AAAACTTTGGAGTTCACTTG+AGG + chr4.1:39398907-39398926 None:intergenic 35.0%
!! AAGACTCTTCTAGGATTAGT+TGG - chr4.1:39399814-39399833 MS.gene08651:CDS 35.0%
!! GAGGATCTTGATTGTTTTTC+AGG - chr4.1:39401871-39401890 MS.gene08651:CDS 35.0%
!! GGTTGTTGGTGTTAAATTCA+TGG - chr4.1:39399008-39399027 MS.gene08651:intron 35.0%
!! TGATTCTGTGGTGTTTTTGA+GGG - chr4.1:39399398-39399417 MS.gene08651:intron 35.0%
!! TTCACCACTGATTCTTTGTA+AGG - chr4.1:39398987-39399006 MS.gene08651:intron 35.0%
!! TTTTAAGTAAACAAGCGCCT+TGG - chr4.1:39401406-39401425 MS.gene08651:CDS 35.0%
AAAGGGCTGAAATGAATGAC+TGG + chr4.1:39400607-39400626 None:intergenic 40.0%
AAATTGGTGAAGAACCCTTC+AGG - chr4.1:39401370-39401389 MS.gene08651:CDS 40.0%
AACATCTCCCTACTTAACAC+AGG + chr4.1:39400395-39400414 None:intergenic 40.0%
AATGCTCCTGCATGTATACA+GGG - chr4.1:39400754-39400773 MS.gene08651:intron 40.0%
ACCCTTGACTTATCACTTCT+TGG - chr4.1:39401243-39401262 MS.gene08651:CDS 40.0%
ACTACTCTTGGATTAATGGG+GGG - chr4.1:39401683-39401702 MS.gene08651:CDS 40.0%
AGACAAAGAGAAGTGGTTCT+TGG - chr4.1:39401113-39401132 MS.gene08651:CDS 40.0%
ATACTAATTCGACCAACCAC+GGG + chr4.1:39400576-39400595 None:intergenic 40.0%
ATATTATTGCCCAGATTGCG+GGG - chr4.1:39400307-39400326 MS.gene08651:intron 40.0%
ATCTGATGCAAACAATGGTG+GGG - chr4.1:39399173-39399192 MS.gene08651:intron 40.0%
ATGAGGATTCAATGGTGTTC+TGG - chr4.1:39399762-39399781 MS.gene08651:CDS 40.0%
CAATCTGATGCAAACAATGG+TGG - chr4.1:39399171-39399190 MS.gene08651:intron 40.0%
CAGGCATGATAAGCTTTCTT+TGG + chr4.1:39400935-39400954 None:intergenic 40.0%
CATTGCTAGTCGTGATATTG+TGG - chr4.1:39400221-39400240 MS.gene08651:CDS 40.0%
CCTACTACTACAGGTTACAT+GGG - chr4.1:39400513-39400532 MS.gene08651:intron 40.0%
CTAATGCTGCTGAGTTTAGT+TGG - chr4.1:39401160-39401179 MS.gene08651:CDS 40.0%
CTCTGGTGTTTGTGGTTATA+TGG - chr4.1:39400034-39400053 MS.gene08651:CDS 40.0%
GAGAATGGTGAATTGTGTGT+TGG - chr4.1:39399423-39399442 MS.gene08651:CDS 40.0%
GATACTAATTCGACCAACCA+CGG + chr4.1:39400577-39400596 None:intergenic 40.0%
GATATTATTGCCCAGATTGC+GGG - chr4.1:39400306-39400325 MS.gene08651:intron 40.0%
GCAAAAACCTCATCAGTTTC+AGG + chr4.1:39399039-39399058 None:intergenic 40.0%
GTTTAGTTGGCAACTTGGAT+TGG - chr4.1:39401173-39401192 MS.gene08651:CDS 40.0%
TATCATGCCTGTTGAGTATC+GGG - chr4.1:39400944-39400963 MS.gene08651:CDS 40.0%
TCACAAAGGTGAACATGGAT+TGG - chr4.1:39401911-39401930 MS.gene08651:CDS 40.0%
TCAGAGCTTGGTTATGATGA+AGG - chr4.1:39399087-39399106 MS.gene08651:intron 40.0%
TCTGACATATTCAAGGACCA+AGG + chr4.1:39401426-39401445 None:intergenic 40.0%
TGAAGCCTTAACACAGAACT+CGG + chr4.1:39399727-39399746 None:intergenic 40.0%
TGAGGATTCAATGGTGTTCT+GGG - chr4.1:39399763-39399782 MS.gene08651:CDS 40.0%
TGGATAACAGGAACTCACAA+AGG - chr4.1:39401897-39401916 MS.gene08651:CDS 40.0%
TGGTCCTTGAATATGTCAGA+CGG - chr4.1:39401426-39401445 MS.gene08651:CDS 40.0%
TGGTCTTTAAACGTGTGTGT+CGG + chr4.1:39401600-39401619 None:intergenic 40.0%
TTCTTGGAGTCAGAAGATGT+TGG - chr4.1:39401216-39401235 MS.gene08651:CDS 40.0%
! ATCTTTTCCCGATACTCAAC+AGG + chr4.1:39400954-39400973 None:intergenic 40.0%
! TCTTGGATGCATTTTCAGCT+GGG - chr4.1:39401130-39401149 MS.gene08651:CDS 40.0%
! TGGCAACTTGGATTGGATAT+TGG - chr4.1:39401180-39401199 MS.gene08651:CDS 40.0%
! TGGTCATTGCAAGGTTTTCT+TGG - chr4.1:39401200-39401219 MS.gene08651:CDS 40.0%
! TTCTTGGATGCATTTTCAGC+TGG - chr4.1:39401129-39401148 MS.gene08651:CDS 40.0%
! TTGATGGTTCATCTTGTGGA+TGG - chr4.1:39399481-39399500 MS.gene08651:CDS 40.0%
!! ACAGGTGCTGTTAAGCAAAT+TGG - chr4.1:39401354-39401373 MS.gene08651:CDS 40.0%
!! GGAAAGTGAGAAAGGGTATT+TGG - chr4.1:39398837-39398856 MS.gene08651:CDS 40.0%
!! GGTTTTGATGGTTCATCTTG+TGG - chr4.1:39399477-39399496 MS.gene08651:CDS 40.0%
!! GTGATTCTGTGGTGTTTTTG+AGG - chr4.1:39399397-39399416 MS.gene08651:intron 40.0%
!! GTGTCTTTTGCTAGTCTGTT+TGG - chr4.1:39401499-39401518 MS.gene08651:CDS 40.0%
!! TATCTTTGAGATCGCAACTG+AGG + chr4.1:39400275-39400294 None:intergenic 40.0%
!! TCAAGGTGCTGAATGTTAGT+AGG + chr4.1:39400859-39400878 None:intergenic 40.0%
!! TTTTGCTAGTCTGTTTGGTC+CGG - chr4.1:39401504-39401523 MS.gene08651:CDS 40.0%
!!! CTTGATTGTTTTTCAGGGCA+TGG - chr4.1:39401877-39401896 MS.gene08651:CDS 40.0%
!!! GTTTTTTACCGTGATGCAAC+AGG - chr4.1:39401336-39401355 MS.gene08651:CDS 40.0%
!!! TTTTTCAGGGCATGGATAAC+AGG - chr4.1:39401885-39401904 MS.gene08651:CDS 40.0%
AAAAAGTTCACCCCGCAATC+TGG + chr4.1:39400320-39400339 None:intergenic 45.0%
AAAAGTTCACCCCGCAATCT+GGG + chr4.1:39400319-39400338 None:intergenic 45.0%
AAACCAAAGAAGGGGCTGTT+TGG + chr4.1:39400727-39400746 None:intergenic 45.0%
ACCACCGTCTGACATATTCA+AGG + chr4.1:39401433-39401452 None:intergenic 45.0%
AGCACCTTGATGTGCATACT+AGG - chr4.1:39400869-39400888 MS.gene08651:intron 45.0%
AGCTCAATTGTCATACTGCG+AGG - chr4.1:39400182-39400201 MS.gene08651:CDS 45.0%
AGGCTGCTATGAGGATTCAA+TGG - chr4.1:39399754-39399773 MS.gene08651:CDS 45.0%
AGGTTGTTGATCCTATTCGC+TGG - chr4.1:39399862-39399881 MS.gene08651:CDS 45.0%
AGTGAGGGCTGAAGATGTTA+TGG - chr4.1:39399635-39399654 MS.gene08651:CDS 45.0%
ATGGTCATCTCTGGTGTTTG+TGG - chr4.1:39400026-39400045 MS.gene08651:CDS 45.0%
CAATGCTCCTGCATGTATAC+AGG - chr4.1:39400753-39400772 MS.gene08651:intron 45.0%
CAGGCAGCAAAAATGTGAGA+GGG - chr4.1:39401769-39401788 MS.gene08651:CDS 45.0%
CCCATGTAACCTGTAGTAGT+AGG + chr4.1:39400516-39400535 None:intergenic 45.0%
CTTGGTTATGATGAAGGTGG+TGG - chr4.1:39399093-39399112 MS.gene08651:intron 45.0%
CTTTCCAAGGCTTGATTACG+CGG - chr4.1:39399230-39399249 MS.gene08651:intron 45.0%
GAAGCCTTAACACAGAACTC+GGG + chr4.1:39399726-39399745 None:intergenic 45.0%
GAGCTTGGTTATGATGAAGG+TGG - chr4.1:39399090-39399109 MS.gene08651:intron 45.0%
GCCTACTACTACAGGTTACA+TGG - chr4.1:39400512-39400531 MS.gene08651:intron 45.0%
GCTGAGTTTAGTTGGCAACT+TGG - chr4.1:39401168-39401187 MS.gene08651:CDS 45.0%
GGAACTCACAAAGGTGAACA+TGG - chr4.1:39401906-39401925 MS.gene08651:CDS 45.0%
GGAATTCGTAGGGCGAAAAA+GGG - chr4.1:39399444-39399463 MS.gene08651:CDS 45.0%
GGGAACAATTGCTTCTGTTC+AGG - chr4.1:39399842-39399861 MS.gene08651:CDS 45.0%
GGTATTTGGATCCACAGCTA+TGG - chr4.1:39398851-39398870 MS.gene08651:CDS 45.0%
TAACAGCACCTGTTGCATCA+CGG + chr4.1:39401347-39401366 None:intergenic 45.0%
TCAGGCAGCAAAAATGTGAG+AGG - chr4.1:39401768-39401787 MS.gene08651:CDS 45.0%
TCCTTGAATATGTCAGACGG+TGG - chr4.1:39401429-39401448 MS.gene08651:CDS 45.0%
TCTGATGCAAACAATGGTGG+GGG - chr4.1:39399174-39399193 MS.gene08651:intron 45.0%
TCTTCCTTGGTGGAGTGAAA+GGG + chr4.1:39400624-39400643 None:intergenic 45.0%
TGACGGAAACTGAACATCGA+GGG + chr4.1:39400677-39400696 None:intergenic 45.0%
TGGAATTCGTAGGGCGAAAA+AGG - chr4.1:39399443-39399462 MS.gene08651:CDS 45.0%
TGGAGTCAGAAGATGTTGGA+AGG - chr4.1:39401220-39401239 MS.gene08651:CDS 45.0%
TGTTAAGGCTTCAGCTGTGA+AGG - chr4.1:39399734-39399753 MS.gene08651:CDS 45.0%
TTCTTCCTTGGTGGAGTGAA+AGG + chr4.1:39400625-39400644 None:intergenic 45.0%
! CCTTTGAGGAATTCAGAGCT+TGG - chr4.1:39399075-39399094 MS.gene08651:intron 45.0%
! GATGCATTTTCAGCTGGGAA+AGG - chr4.1:39401135-39401154 MS.gene08651:CDS 45.0%
! TCATCTTGTGGATGGACTTC+AGG - chr4.1:39399489-39399508 MS.gene08651:CDS 45.0%
!! AACCAGAAGAAGCTTGTTGC+TGG - chr4.1:39399375-39399394 MS.gene08651:intron 45.0%
!! ACGGTGAGCTTTGGAAGTTT+AGG - chr4.1:39399292-39399311 MS.gene08651:intron 45.0%
!! CCAAGCTCTGAATTCCTCAA+AGG + chr4.1:39399078-39399097 None:intergenic 45.0%
!! GTGTTTTTGAGGGCTGAGAA+TGG - chr4.1:39399408-39399427 MS.gene08651:intron 45.0%
!! TTCAATGGTGTTCTGGGATG+AGG - chr4.1:39399769-39399788 MS.gene08651:CDS 45.0%
ACCCTGTTGTAAGCAAGTGC+CGG + chr4.1:39399341-39399360 None:intergenic 50.0%
ACGGAAACTGAACATCGAGG+GGG + chr4.1:39400675-39400694 None:intergenic 50.0%
AGCTGGTTGCCTGAAAACGT+CGG + chr4.1:39400700-39400719 None:intergenic 50.0%
ATCGTCTCAGCACAGTACCT+TGG + chr4.1:39399210-39399229 None:intergenic 50.0%
CACCAGCAACAAGCTTCTTC+TGG + chr4.1:39399380-39399399 None:intergenic 50.0%
CACTCCCGAGTTCTGTGTTA+AGG - chr4.1:39399719-39399738 MS.gene08651:CDS 50.0%
CGTAGGGCGAAAAAGGGAAT+CGG - chr4.1:39399450-39399469 MS.gene08651:CDS 50.0%
GACGGAAACTGAACATCGAG+GGG + chr4.1:39400676-39400695 None:intergenic 50.0%
GAGTGGAAGAGTGAGAGTGA+GGG - chr4.1:39399620-39399639 MS.gene08651:CDS 50.0%
GCATGTTCAGCATGTCCTTG+TGG + chr4.1:39398937-39398956 None:intergenic 50.0%
GGAGAATTAGGCCAGCGAAT+AGG + chr4.1:39399876-39399895 None:intergenic 50.0%
GTGCTGAGACGATCTTTCCA+AGG - chr4.1:39399217-39399236 MS.gene08651:intron 50.0%
TCCTCCAAATTGAGCATGCC+TGG + chr4.1:39400782-39400801 None:intergenic 50.0%
TGAGTGGAAGAGTGAGAGTG+AGG - chr4.1:39399619-39399638 MS.gene08651:CDS 50.0%
TTGCCTGAAAACGTCGGTGA+CGG + chr4.1:39400694-39400713 None:intergenic 50.0%
! CTGATGCAAACAATGGTGGG+GGG - chr4.1:39399175-39399194 MS.gene08651:intron 50.0%
! GCTTGTTGCTGGTGATTCTG+TGG - chr4.1:39399386-39399405 MS.gene08651:intron 50.0%
! GGTTATGATGAAGGTGGTGG+TGG - chr4.1:39399096-39399115 MS.gene08651:intron 50.0%
! GTGACGGAAACTGAACATCG+AGG + chr4.1:39400678-39400697 None:intergenic 50.0%
! TGTGGATGGACTTCAGGTTC+TGG - chr4.1:39399495-39399514 MS.gene08651:CDS 50.0%
! TTTCCGTCACCGACGTTTTC+AGG - chr4.1:39400688-39400707 MS.gene08651:intron 50.0%
!! AATACCTGAAGAAGGCGCCA+CGG + chr4.1:39399897-39399916 None:intergenic 50.0%
!! ACGTTTTCAGGCAACCAGCT+CGG - chr4.1:39400700-39400719 MS.gene08651:intron 50.0%
!!! GGAATCGGTGGTGGTTTTGA+TGG - chr4.1:39399465-39399484 MS.gene08651:CDS 50.0%
AACCGCAGCTTCTTCCTTGG+TGG + chr4.1:39400634-39400653 None:intergenic 55.0%
AGGATGTGCACGGTGAGCTT+TGG - chr4.1:39399283-39399302 MS.gene08651:intron 55.0%
AGGGCGAAAAAGGGAATCGG+TGG - chr4.1:39399453-39399472 MS.gene08651:CDS 55.0%
CAGCTATGGCATGCTTGTGC+TGG - chr4.1:39398865-39398884 MS.gene08651:CDS 55.0%
CAGCTGTGAAGGCTGCTATG+AGG - chr4.1:39399745-39399764 MS.gene08651:CDS 55.0%
CTATGGCATGCTTGTGCTGG+TGG - chr4.1:39398868-39398887 MS.gene08651:CDS 55.0%
CTCCACCAAGGAAGAAGCTG+CGG - chr4.1:39400629-39400648 MS.gene08651:intron 55.0%
CTGCATGTATACAGGGAGCC+AGG - chr4.1:39400761-39400780 MS.gene08651:intron 55.0%
CTGGCTCCCTGTATACATGC+AGG + chr4.1:39400763-39400782 None:intergenic 55.0%
GCACAAGCATGCCATAGCTG+TGG + chr4.1:39398865-39398884 None:intergenic 55.0%
GCCAGGCATGCTCAATTTGG+AGG - chr4.1:39400778-39400797 MS.gene08651:intron 55.0%
GCCGGCACTTGCTTACAACA+GGG - chr4.1:39399337-39399356 MS.gene08651:intron 55.0%
GCGAAAAAGGGAATCGGTGG+TGG - chr4.1:39399456-39399475 MS.gene08651:CDS 55.0%
GGAAACCGCAGCTTCTTCCT+TGG + chr4.1:39400637-39400656 None:intergenic 55.0%
GGAGCCAGGCATGCTCAATT+TGG - chr4.1:39400775-39400794 MS.gene08651:intron 55.0%
GGCACTTGCTTACAACAGGG+TGG - chr4.1:39399340-39399359 MS.gene08651:intron 55.0%
GGCTGACCCTGAAACTGATG+AGG - chr4.1:39399029-39399048 MS.gene08651:CDS 55.0%
TCAGCCCTTTCACTCCACCA+AGG - chr4.1:39400617-39400636 MS.gene08651:intron 55.0%
TCAGCCGCGTAATCAAGCCT+TGG + chr4.1:39399237-39399256 None:intergenic 55.0%
TTTATAGAGGGACACCGCGC+CGG - chr4.1:39399319-39399338 MS.gene08651:intron 55.0%
! GCATGCTTGTGCTGGTGGAA+TGG - chr4.1:39398873-39398892 MS.gene08651:CDS 55.0%
! GTGGGGGGTTTTCTGTTCCA+AGG - chr4.1:39399190-39399209 MS.gene08651:intron 55.0%
! TTCGCTGGCCTAATTCTCCG+TGG - chr4.1:39399877-39399896 MS.gene08651:CDS 55.0%
!! AACTCGGGAGTGCTAGCTCT+TGG + chr4.1:39399711-39399730 None:intergenic 55.0%
!! AGAAGGCGCCACGGAGAATT+AGG + chr4.1:39399888-39399907 None:intergenic 55.0%
!! AGGTGGTGGTGGTGATGGTT+TGG - chr4.1:39399107-39399126 MS.gene08651:intron 55.0%
!! CGTTTTCAGGCAACCAGCTC+GGG - chr4.1:39400701-39400720 MS.gene08651:intron 55.0%
!! GATGAAGGTGGTGGTGGTGA+TGG - chr4.1:39399102-39399121 MS.gene08651:intron 55.0%
ACGTGAAGCGTGTTAGCCCG+TGG - chr4.1:39400557-39400576 MS.gene08651:intron 60.0%
AGGGGGAAGTCGAGGTTTGG+TGG + chr4.1:39400658-39400677 None:intergenic 60.0%
CGCCGGCACTTGCTTACAAC+AGG - chr4.1:39399336-39399355 MS.gene08651:intron 60.0%
GAACATCGAGGGGGAAGTCG+AGG + chr4.1:39400666-39400685 None:intergenic 60.0%
GAAGCGTGTTAGCCCGTGGT+TGG - chr4.1:39400561-39400580 MS.gene08651:intron 60.0%
GGGCCAAACAGCCCCTTCTT+TGG - chr4.1:39400721-39400740 MS.gene08651:intron 60.0%
TCGAGGGGGAAGTCGAGGTT+TGG + chr4.1:39400661-39400680 None:intergenic 60.0%
TTCGCCACCACCGTCTGAAC+AGG + chr4.1:39399264-39399283 None:intergenic 60.0%
TTCTCCGTGGCGCCTTCTTC+AGG - chr4.1:39399890-39399909 MS.gene08651:CDS 60.0%
! GTGGTGGCGAAGGATGTGCA+CGG - chr4.1:39399273-39399292 MS.gene08651:intron 60.0%
! TGTTCAGACGGTGGTGGCGA+AGG - chr4.1:39399263-39399282 MS.gene08651:intron 60.0%
!! GTTGTAAGCAAGTGCCGGCG+CGG + chr4.1:39399336-39399355 None:intergenic 60.0%
GCCACCACCGTCTGAACAGG+CGG + chr4.1:39399261-39399280 None:intergenic 65.0%
GGCTGAGCCGCCTGTTCAGA+CGG - chr4.1:39399251-39399270 MS.gene08651:intron 65.0%
TGAGCCGCCTGTTCAGACGG+TGG - chr4.1:39399254-39399273 MS.gene08651:intron 65.0%
AGGGGCTGTTTGGCCCGAGC+TGG + chr4.1:39400717-39400736 None:intergenic 70.0%
GCCGCCTGTTCAGACGGTGG+TGG - chr4.1:39399257-39399276 MS.gene08651:intron 70.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 39398832 39401974 39398832 ID=MS.gene08651
chr4.1 mRNA 39398832 39401974 39398832 ID=MS.gene08651.t1;Parent=MS.gene08651
chr4.1 exon 39400895 39401974 39400895 ID=MS.gene08651.t1.exon1;Parent=MS.gene08651.t1
chr4.1 CDS 39400895 39401974 39400895 ID=cds.MS.gene08651.t1;Parent=MS.gene08651.t1
chr4.1 exon 39399415 39400280 39399415 ID=MS.gene08651.t1.exon2;Parent=MS.gene08651.t1
chr4.1 CDS 39399415 39400280 39399415 ID=cds.MS.gene08651.t1;Parent=MS.gene08651.t1
chr4.1 exon 39399016 39399081 39399016 ID=MS.gene08651.t1.exon3;Parent=MS.gene08651.t1
chr4.1 CDS 39399016 39399081 39399016 ID=cds.MS.gene08651.t1;Parent=MS.gene08651.t1
chr4.1 exon 39398832 39398913 39398832 ID=MS.gene08651.t1.exon4;Parent=MS.gene08651.t1
chr4.1 CDS 39398832 39398913 39398832 ID=cds.MS.gene08651.t1;Parent=MS.gene08651.t1
Gene Sequence

>MS.gene08651

ATGAAGGAAAGTGAGAAAGGGTATTTGGATCCACAGCTATGGCATGCTTGTGCTGGTGGAATGGTTCAGATACCTCAAGTGAACTCCAAAGTTTTCTACTTTCCACAAGGACATGCTGAACATGCTAATACAAACATTGATCTAAGGGTTTCAGTTTCACCACTGATTCTTTGTAAGGTTGTTGGTGTTAAATTCATGGCTGACCCTGAAACTGATGAGGTTTTTGCTGTAATGAAGCTTCTTCCTTTGAGGAATTCAGAGCTTGGTTATGATGAAGGTGGTGGTGGTGATGGTTTGGAAAATTCAGAAAAGCCTGCATCTTTTGCTAAGACTTTGACTCAATCTGATGCAAACAATGGTGGGGGGTTTTCTGTTCCAAGGTACTGTGCTGAGACGATCTTTCCAAGGCTTGATTACGCGGCTGAGCCGCCTGTTCAGACGGTGGTGGCGAAGGATGTGCACGGTGAGCTTTGGAAGTTTAGGCATATTTATAGAGGGACACCGCGCCGGCACTTGCTTACAACAGGGTGGAGTAGTTTTGTGAACCAGAAGAAGCTTGTTGCTGGTGATTCTGTGGTGTTTTTGAGGGCTGAGAATGGTGAATTGTGTGTTGGAATTCGTAGGGCGAAAAAGGGAATCGGTGGTGGTTTTGATGGTTCATCTTGTGGATGGACTTCAGGTTCTGGTTCTAACAATGTAAATTGTGGAATTGGACCTTTTGGAGCATTTTCTTTTTTCTTGAAAGAAGAGAATAAGACATTGAGAAATGGTTGTTTTGATGGTAATTTGAGTGGAAGAGTGAGAGTGAGGGCTGAAGATGTTATGGAAGCTGTGAAATTGGCTGCTAATAACCAACCATTTGAAGTTGTTTACTATCCAAGAGCTAGCACTCCCGAGTTCTGTGTTAAGGCTTCAGCTGTGAAGGCTGCTATGAGGATTCAATGGTGTTCTGGGATGAGGTTCAAGATGCCCTTTGAAACTGAAGACTCTTCTAGGATTAGTTGGTTTATGGGAACAATTGCTTCTGTTCAGGTTGTTGATCCTATTCGCTGGCCTAATTCTCCGTGGCGCCTTCTTCAGGTATTGTTTTTTAGTTACAATTTGGAGAAAATATGTTGAACACATCTTTACATAAGGGACTCAATTTTTATGCTTCTTTGGATATCTATCTGTGCTTTGTTATTGTTCATTTTTATGGTCATCTCTGGTGTTTGTGGTTATATGGTGTAAATATCTGTAAAAACAAGTCGATTTAGCTGAAATCATAGGTATAATTTTTCAATCAACCTCCTATACTACTAATTGTTTCATTTATTCGTGCACTAATTCAAGTGAAATTCTTGAAAAAATAGCTCAATTGTCATACTGCGAGGTTTTAAATTGCAGTCGCATTGCTAGTCGTGATATTGTGGATAATCACAGGCAAATGTATCTATTACGCCTCAGTTGCGATCTCAAAGATATCTAAATCCTTGATATTATTGCCCAGATTGCGGGGTGAACTTTTTTTCAAACTCTTGGTTTTTTTTTTTCACTCTTGTGATTATGAGGATGAAATAACCTGTGTTAAGTAGGGAGATGTTTTTGCATTCTTGATTGCATTCACATCTTAGTTCAAAAATCAATTTAGTTTTGCACAGAATCATATCACACATTCTTGATTATCGATCTCATTGTTATGCCTACTACTACAGGTTACATGGGATGAGCCTGATTTACTACAAAACGTGAAGCGTGTTAGCCCGTGGTTGGTCGAATTAGTATCAAATGTACCAGTCATTCATTTCAGCCCTTTCACTCCACCAAGGAAGAAGCTGCGGTTTCCACCAAACCTCGACTTCCCCCTCGATGTTCAGTTTCCGTCACCGACGTTTTCAGGCAACCAGCTCGGGCCAAACAGCCCCTTCTTTGGTTTATCTGACAATGCTCCTGCATGTATACAGGGAGCCAGGCATGCTCAATTTGGAGGAATATCTCTTTCAGATCTCAACCTTAACAACAATAAACTTCAGTTAGGGTTGTTTCCTACTAACATTCAGCACCTTGATGTGCATACTAGGATTTCAAATAGCAACAACATGATGAACAACAATGATAAGACCAAAGAAAGCTTATCATGCCTGTTGAGTATCGGGAAAAGATATGATAAGAGTTCAGAGAAATCTCAATCTGATGACAATGTTGTTAAGAAACATCAGTTTTGTCTTTTTGGTCAGCCAATACTCACTGAGCAACAAATTTCTAGAAACTGTTCTGAAAAAAATTCAATAGATGATAGTAAAGACAAAGAGAAGTGGTTCTTGGATGCATTTTCAGCTGGGAAAGGTTCTAATGCTGCTGAGTTTAGTTGGCAACTTGGATTGGATATTGGTCATTGCAAGGTTTTCTTGGAGTCAGAAGATGTTGGAAGGACCCTTGACTTATCACTTCTTGGTTCTTATGAAGAGCTCTACAAGAAACTTGCTAACATGTTTGATTTAGAAAAGTCTGAGATGTTGAATCATGTTTTTTACCGTGATGCAACAGGTGCTGTTAAGCAAATTGGTGAAGAACCCTTCAGGTATTGCTTTTGTGTTTTAAGTAAACAAGCGCCTTGGTCCTTGAATATGTCAGACGGTGGTACTATAGTCTCTGAATGTATCAAAATTATAAAAAACATATCTAAATGTGTCTTTTGCTAGTCTGTTTGGTCCGGTTGTTAGTTTAGTCTACAAAATTACTATGTTTTTTTAAGTTTCACAATACATTCTAGGACTATGATGACACCGACACACACGTTTAAAGACCAAAATGACTTCACTCTATTTTTTTTCTTTTGAATTTTCCCATTATTGTACAGTAGCAAAATAGTACTACTCTTGGATTAATGGGGGGTTTTGTGATCTCATTGCAGTGAATTCATGAAGACAGCAAAAAGATTGACAATTCTGACAGATTCAGGCAGCAAAAATGTGAGAGGGTAAGAGAGAAAATGAGTTCATCAGTTTTGTTTTATTCATCTGTGTAAATGATTATGTGTTGAAATGTCTTATATAATAAGAGGATCTTGATTGTTTTTCAGGGCATGGATAACAGGAACTCACAAAGGTGAACATGGATTGGATGCATCAAACAAAACAGGACCTCTAAGCATATTTGCATAA
Protein sequence

>MS.gene08651.t1

MKESEKGYLDPQLWHACAGGMVQIPQVNSKVFYFPQGHAEHANTNIDLRVSVSPLILCKVVGVKFMADPETDEVFAVMKLLPLRNSELGYDEGGGGDGLENSEKPASFAKTLTQSDANNGGGFSVPRYCAETIFPRLDYAAEPPVQTVVAKDVHGELWKFRHIYRGTPRRHLLTTGWSSFVNQKKLVAGDSVVFLRAENGELCVGIRRAKKGIGGGFDGSSCGWTSGSGSNNVNCGIGPFGAFSFFLKEENKTLRNGCFDGNLSGRVRVRAEDVMEAVKLAANNQPFEVVYYPRASTPEFCVKASAVKAAMRIQWCSGMRFKMPFETEDSSRISWFMGTIASVQVVDPIRWPNSPWRLLQVTWDEPDLLQNVKRVSPWLVELVSNVPVIHFSPFTPPRKKLRFPPNLDFPLDVQFPSPTFSGNQLGPNSPFFGLSDNAPACIQGARHAQFGGISLSDLNLNNNKLQLGLFPTNIQHLDVHTRISNSNNMMNNNDKTKESLSCLLSIGKRYDKSSEKSQSDDNVVKKHQFCLFGQPILTEQQISRNCSEKNSIDDSKDKEKWFLDAFSAGKGSNAAEFSWQLGLDIGHCKVFLESEDVGRTLDLSLLGSYEELYKKLANMFDLEKSEMLNHVFYRDATGAVKQIGEEPFSEFMKTAKRLTILTDSGSKNVRGAWITGTHKGEHGLDASNKTGPLSIFA