AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08758

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08758.t1 MTR_8g085980 100.000 337 0 0 35 371 113 449 0.0 703
MS.gene08758.t1 MTR_7g085800 95.719 327 14 0 35 361 113 439 0.0 632
MS.gene08758.t1 MTR_1g106005 92.308 338 25 1 35 371 113 450 0.0 629
MS.gene08758.t1 MTR_3g110720 94.479 326 18 0 35 360 113 438 0.0 626
MS.gene08758.t1 MTR_4g097830 93.884 327 20 0 35 361 113 439 0.0 625
MS.gene08758.t1 MTR_6g029190 87.537 337 40 1 35 371 114 448 0.0 595
MS.gene08758.t1 MTR_2g041670 74.455 321 82 0 36 356 109 429 1.58e-170 484
MS.gene08758.t1 MTR_2g041400 74.367 316 81 0 36 351 109 424 8.75e-167 473
MS.gene08758.t1 MTR_3g063410 90.260 154 15 0 174 327 24 177 5.18e-106 310
MS.gene08758.t1 MTR_3g063410 89.855 138 14 0 190 327 1 138 3.04e-92 273
MS.gene08758.t1 MTR_4g094535 41.304 322 181 1 35 356 111 424 8.97e-87 270
MS.gene08758.t1 MTR_2g031310 40.994 322 182 1 35 356 111 424 3.95e-86 268
MS.gene08758.t1 MTR_8g107250 39.763 337 193 2 35 371 112 438 6.96e-86 267
MS.gene08758.t1 MTR_4g017630 40.994 322 182 1 35 356 111 424 8.17e-86 267
MS.gene08758.t1 MTR_1g101130 40.683 322 183 1 35 356 111 424 1.27e-85 266
MS.gene08758.t1 MTR_4g019090 40.683 322 183 1 35 356 111 424 2.85e-85 266
MS.gene08758.t1 MTR_3g113970 40.373 322 184 1 35 356 113 426 2.90e-85 266
MS.gene08758.t1 MTR_7g089120 40.683 322 183 2 35 356 111 424 9.21e-85 265
MS.gene08758.t1 MTR_4g019110 40.994 322 182 1 35 356 111 424 2.73e-84 263
MS.gene08758.t1 MTR_8g098360 39.130 322 188 1 35 356 111 424 3.48e-84 263
MS.gene08758.t1 MTR_7g117050 28.528 326 217 5 41 355 119 439 2.04e-47 167
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08758.t1 AT5G19780 98.521 338 4 1 35 371 113 450 0.0 692
MS.gene08758.t1 AT5G19770 98.521 338 4 1 35 371 113 450 0.0 692
MS.gene08758.t1 AT4G14960 93.808 323 20 0 35 357 113 435 0.0 618
MS.gene08758.t1 AT1G04820 93.808 323 20 0 35 357 113 435 0.0 617
MS.gene08758.t1 AT1G50010 93.808 323 20 0 35 357 113 435 0.0 617
MS.gene08758.t1 AT1G64740 94.118 323 19 0 35 357 113 435 0.0 615
MS.gene08758.t1 AT4G14960 87.458 295 30 1 35 329 113 400 0.0 516
MS.gene08758.t1 AT1G75780 40.683 322 183 1 35 356 112 425 8.16e-87 270
MS.gene08758.t1 AT1G20010 40.683 322 183 1 35 356 112 425 4.07e-86 268
MS.gene08758.t1 AT5G62700 40.683 322 183 1 35 356 111 424 9.49e-86 267
MS.gene08758.t1 AT5G62690 40.683 322 183 1 35 356 111 424 9.49e-86 267
MS.gene08758.t1 AT5G23860 40.683 322 183 1 35 356 111 424 1.82e-85 266
MS.gene08758.t1 AT5G23860 40.683 322 183 1 35 356 111 424 1.82e-85 266
MS.gene08758.t1 AT2G29550 40.373 322 184 1 35 356 111 424 1.94e-85 266
MS.gene08758.t1 AT4G20890 40.683 322 183 2 35 356 111 424 3.70e-85 265
MS.gene08758.t1 AT5G12250 40.683 322 183 2 35 356 111 424 8.57e-85 265
MS.gene08758.t1 AT5G44340 40.683 322 183 2 35 356 111 424 5.11e-84 262
MS.gene08758.t1 AT5G05620 29.141 326 215 5 41 355 119 439 4.95e-48 169
MS.gene08758.t1 AT3G61650 28.528 326 217 5 41 355 119 439 9.04e-47 166

Find 106 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACGTCTTTCTGTTGATTA+TGG 0.176344 4.2:+12608383 MS.gene08758:CDS
AGGTTTGGGTTCACTTCTTT+TGG 0.254943 4.2:+12608361 MS.gene08758:CDS
GGTGGAACCGGTTCAGGTTT+GGG 0.262975 4.2:+12608347 MS.gene08758:CDS
CAGCCTCCTTCAGTTGTTCC+TGG 0.319747 4.2:+12609437 MS.gene08758:CDS
TTGCCGGAGATGAGTTGTTC+AGG 0.320827 4.2:-12608102 None:intergenic
AACACAGCATTGGTAATTTC+CGG 0.327913 4.2:-12608932 None:intergenic
TGGTGGAACCGGTTCAGGTT+TGG 0.328516 4.2:+12608346 MS.gene08758:CDS
TTTGGATCGTGTTAGGAAAT+TGG 0.329849 4.2:+12608274 MS.gene08758:CDS
GTTGGTGGTGGAACCGGTTC+AGG 0.343909 4.2:+12608341 MS.gene08758:CDS
ACCACCTCGTTGAGGTTTGA+TGG 0.345949 4.2:+12608782 MS.gene08758:CDS
ATCTGTGTTTGGATCGTGTT+AGG 0.350514 4.2:+12608267 MS.gene08758:CDS
CACTGATGTGGCGGTGCTCT+TGG 0.354267 4.2:+12608658 MS.gene08758:CDS
AACGCTGTTGGTGGTGGAAC+CGG 0.370166 4.2:+12608335 MS.gene08758:CDS
GTAGATGGTGAATCCAAGTT+TGG 0.394713 4.2:-12608414 None:intergenic
ACAGCAGCATTGACATCCTT+TGG 0.397105 4.2:-12609040 None:intergenic
GGAAATTGTAGATCTGTGTT+TGG 0.411361 4.2:+12608256 MS.gene08758:intron
GCCTTAGCAGCAGAGATAAC+AGG 0.413078 4.2:-12608887 None:intergenic
CCAGTGGACAAAGGCTCTCT+TGG 0.425867 4.2:-12609564 None:intergenic
AGGCGATCTATGACATTTGT+AGG 0.426144 4.2:+12608687 MS.gene08758:CDS
GTTAGCGGCGTCTTCTTTGC+CGG 0.427769 4.2:-12608118 None:intergenic
GCCATATATTTCCCATGCCT+TGG 0.429047 4.2:-12608986 None:intergenic
CTAGGTTTCAACTGCTGTTG+TGG 0.430726 4.2:+12608592 MS.gene08758:intron
CAACTTATACCAACTTGAAC+AGG 0.431265 4.2:+12608732 MS.gene08758:CDS
TCCAAGGCATGGGAAATATA+TGG 0.435927 4.2:+12608985 MS.gene08758:CDS
ATGTATGACAGGTGCCCAAC+TGG 0.444943 4.2:+12609392 MS.gene08758:intron
GATAACTGTACCGGTCTTCA+AGG 0.448969 4.2:+12608299 MS.gene08758:CDS
GTCGGTGAGGGCATGGAAGA+AGG 0.451862 4.2:+12609590 MS.gene08758:CDS
AAGTGAACCCAAACCTGAAC+CGG 0.452878 4.2:-12608354 None:intergenic
TACCTGAGGGGAAGGGTAGA+TGG 0.468178 4.2:-12608429 None:intergenic
TGCCGGAGATGAGTTGTTCA+GGG 0.468722 4.2:-12608101 None:intergenic
CGAGAGCAGCAAGATCTTCA+CGG 0.470136 4.2:-12609628 None:intergenic
TCGGTGAGGGCATGGAAGAA+GGG 0.478798 4.2:+12609591 MS.gene08758:CDS
CCAAGAGAGCCTTTGTCCAC+TGG 0.491892 4.2:+12609564 MS.gene08758:CDS
ACGAGGATACGGCACAAGAT+TGG 0.495414 4.2:-12608837 None:intergenic
ACCTGTTATCTCTGCTGCTA+AGG 0.495705 4.2:+12608886 MS.gene08758:CDS
CACCATCTACCCTTCCCCTC+AGG 0.496887 4.2:+12608427 MS.gene08758:CDS
GTCATCGATGAAGTTCGTTC+CGG 0.499563 4.2:+12608057 None:intergenic
CTGGTATGTCGGTGAGGGCA+TGG 0.501551 4.2:+12609583 MS.gene08758:CDS
GGAGCAGAAGATGATGAGGA+AGG 0.503402 4.2:+12609680 MS.gene08758:CDS
CTGTTCAAGTTGGTATAAGT+TGG 0.510056 4.2:-12608731 None:intergenic
CACCCTGAACAACTCATCTC+CGG 0.515494 4.2:+12608099 MS.gene08758:CDS
TACCACATTTGAAACCAGTT+GGG 0.518530 4.2:-12609406 None:intergenic
ATCGAACTTGTGATCAATGC+GGG 0.523345 4.2:-12609531 None:intergenic
GAGAAAGATTATGAAGAAGT+TGG 0.523429 4.2:+12609650 MS.gene08758:CDS
GTTCAGGGTGGAAGAGTTGT+CGG 0.523884 4.2:-12608086 None:intergenic
GGACTGTTCAGTTTGTTGAC+TGG 0.529278 4.2:+12609083 MS.gene08758:CDS
TCCTGGCGGTGACCTTGCTA+AGG 0.530128 4.2:+12609454 MS.gene08758:CDS
GAAGAGTTGTCGGTAGGTAC+CGG 0.530553 4.2:-12608076 None:intergenic
AGAAGATGATGAGGAAGGAG+AGG 0.532591 4.2:+12609685 MS.gene08758:CDS
TTATGAAGAAGTTGGTGCTG+AGG 0.534554 4.2:+12609658 MS.gene08758:CDS
GAATGTTGTACCTGAGGGGA+AGG 0.534693 4.2:-12608437 None:intergenic
CAGTAACAACACTGCTGTTG+CGG 0.536971 4.2:+12609499 MS.gene08758:CDS
GTCCACTGGTATGTCGGTGA+GGG 0.540123 4.2:+12609578 MS.gene08758:CDS
CACTTAGCCATCATACTAGC+AGG 0.541426 4.2:-12608959 None:intergenic
GATACGGCACAAGATTGGTC+TGG 0.542354 4.2:-12608832 None:intergenic
TCATGAGCAGCTATCTGTGC+CGG 0.543119 4.2:+12608913 MS.gene08758:CDS
TATGGAAAGAAATCCAAACT+TGG 0.546100 4.2:+12608401 MS.gene08758:CDS
TATGAAGAAGTTGGTGCTGA+GGG 0.548545 4.2:+12609659 MS.gene08758:CDS
ATACCACATTTGAAACCAGT+TGG 0.549643 4.2:-12609407 None:intergenic
CGGTGAGGGCATGGAAGAAG+GGG 0.558712 4.2:+12609592 MS.gene08758:CDS
TCTTCTTGAGCACACTGATG+TGG 0.559183 4.2:+12608646 MS.gene08758:CDS
GAGAGCAGCAAGATCTTCAC+GGG 0.561278 4.2:-12609627 None:intergenic
ACCTTAGCAAGGTCACCGCC+AGG 0.564664 4.2:-12609455 None:intergenic
GCCTTTGTCCACTGGTATGT+CGG 0.567211 4.2:+12609572 MS.gene08758:CDS
ATGAAATGAATACGAGGATA+CGG 0.568430 4.2:-12608848 None:intergenic
TCCTCTAGCGAAATTGTTAG+CGG 0.568659 4.2:-12608133 None:intergenic
AAACCTCAACGAGGTGGTGA+GGG 0.569782 4.2:-12608777 None:intergenic
GTCGATAGAACACTGTTGTA+TGG 0.573851 4.2:-12608617 None:intergenic
GCTAAGTGTGATCCAAGGCA+TGG 0.576706 4.2:+12608974 MS.gene08758:CDS
TGATATCAACCTGTTCAAGT+TGG 0.577755 4.2:-12608741 None:intergenic
GTTTGAGCCTGCTAGTATGA+TGG 0.579221 4.2:+12608952 MS.gene08758:CDS
TGAGGGAGCAGAAGATGATG+AGG 0.580692 4.2:+12609676 MS.gene08758:CDS
CAGCTCTTTGCACCTTAGCA+AGG 0.580894 4.2:-12609466 None:intergenic
ATAACTGTACCGGTCTTCAA+GGG 0.586323 4.2:+12608300 MS.gene08758:CDS
AAATTGGCTGATAACTGTAC+CGG 0.586586 4.2:+12608290 MS.gene08758:CDS
TGCCCAACTGGTTTCAAATG+TGG 0.589673 4.2:+12609404 MS.gene08758:CDS
AGCAGGCTCAAACACAGCAT+TGG 0.589885 4.2:-12608942 None:intergenic
CAAACCTCAACGAGGTGGTG+AGG 0.590642 4.2:-12608778 None:intergenic
TGTCCACTGGTATGTCGGTG+AGG 0.595519 4.2:+12609577 MS.gene08758:CDS
GGCGGTGCTCTTGGATAATG+AGG 0.598449 4.2:+12608667 MS.gene08758:CDS
TCACCGCCAGGAACAACTGA+AGG 0.606748 4.2:-12609443 None:intergenic
GGAGATCTCTTGATATTGAG+AGG 0.607664 4.2:+12608708 MS.gene08758:CDS
CCGTGGTGATGTTGTTCCAA+AGG 0.608496 4.2:+12609024 MS.gene08758:CDS
AATGTTGTACCTGAGGGGAA+GGG 0.614483 4.2:-12608436 None:intergenic
GCCGCTAACAATTTCGCTAG+AGG 0.616891 4.2:+12608132 MS.gene08758:CDS
CTAAGTGTGATCCAAGGCAT+GGG 0.617321 4.2:+12608975 MS.gene08758:CDS
CATCCCTCACCACCTCGTTG+AGG 0.618692 4.2:+12608774 MS.gene08758:CDS
TGCCCTCACCGACATACCAG+TGG 0.624103 4.2:-12609580 None:intergenic
TAACAACACTGCTGTTGCGG+AGG 0.625191 4.2:+12609502 MS.gene08758:CDS
TTCGCTAGAGGACACTACAC+TGG 0.625494 4.2:+12608144 MS.gene08758:CDS
AAGGATGTCAATGCTGCTGT+TGG 0.630667 4.2:+12609043 MS.gene08758:CDS
TCTTGAGCACACTGATGTGG+CGG 0.645120 4.2:+12608649 MS.gene08758:CDS
AGGGTGGAAGAGTTGTCGGT+AGG 0.660011 4.2:-12608082 None:intergenic
TGATGGCTAAGTGTGATCCA+AGG 0.660907 4.2:+12608969 MS.gene08758:CDS
CCTCCTTCAGTTGTTCCTGG+CGG 0.661195 4.2:+12609440 MS.gene08758:CDS
CCGCCAGGAACAACTGAAGG+AGG 0.665389 4.2:-12609440 None:intergenic
TTGGCACCATCAAAACAAAG+AGG 0.672011 4.2:+12609062 MS.gene08758:CDS
ACCGACATACCAGTGGACAA+AGG 0.672853 4.2:-12609573 None:intergenic
ACCATCAAACCTCAACGAGG+TGG 0.684702 4.2:-12608783 None:intergenic
CGGAGATGAGTTGTTCAGGG+TGG 0.684993 4.2:-12608098 None:intergenic
GCTTGCTGTTTGATGTACCG+TGG 0.703831 4.2:+12609007 MS.gene08758:CDS
TCTTGAATGTTGTACCTGAG+GGG 0.708772 4.2:-12608441 None:intergenic
GATCGAACTTGTGATCAATG+CGG 0.718228 4.2:-12609532 None:intergenic
GAAAGCATGAAATGAATACG+AGG 0.732987 4.2:-12608854 None:intergenic
CCTTTGGAACAACATCACCA+CGG 0.768666 4.2:-12609024 None:intergenic
AGCACCATCAAACCTCAACG+AGG 0.774284 4.2:-12608786 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTGTTCTTTTTTTTG+TGG + chr4.2:12608221-12608240 MS.gene08758:intron 15.0%
!! AATAACTATTTGTAGAATGA+CGG + chr4.2:12609119-12609138 MS.gene08758:intron 20.0%
!! AATCATGTTGATGTAAATAT+TGG + chr4.2:12608497-12608516 MS.gene08758:intron 20.0%
!! TTCATATTATAATGTATGAC+AGG + chr4.2:12609381-12609400 MS.gene08758:intron 20.0%
!!! TAATGGTGAATTGTTATTTT+TGG + chr4.2:12608567-12608586 MS.gene08758:intron 20.0%
!!! TTTTATAAGGGTAGATAATT+TGG + chr4.2:12609195-12609214 MS.gene08758:intron 20.0%
!!! ATTGGTTTTTCTGTTTGTAA+TGG + chr4.2:12608550-12608569 MS.gene08758:intron 25.0%
!!! GAATTGTTATTTTTGGATCT+AGG + chr4.2:12608574-12608593 MS.gene08758:intron 25.0%
AAATCTCGAAATTAGGGTTA+GGG - chr4.2:12608187-12608206 None:intergenic 30.0%
AAGCAAGAAATCTCGAAATT+AGG - chr4.2:12608194-12608213 None:intergenic 30.0%
AGCAAGAAATCTCGAAATTA+GGG - chr4.2:12608193-12608212 None:intergenic 30.0%
ATGAAATGAATACGAGGATA+CGG - chr4.2:12608851-12608870 None:intergenic 30.0%
CCTTATAAAATATGCAGCTA+CGG - chr4.2:12609185-12609204 None:intergenic 30.0%
GAGAAAGATTATGAAGAAGT+TGG + chr4.2:12609650-12609669 MS.gene08758:CDS 30.0%
GATTGATGTATAGATCATAG+TGG + chr4.2:12608524-12608543 MS.gene08758:intron 30.0%
TATGGAAAGAAATCCAAACT+TGG + chr4.2:12608401-12608420 MS.gene08758:CDS 30.0%
! CGTAGCTGCATATTTTATAA+GGG + chr4.2:12609183-12609202 MS.gene08758:intron 30.0%
!! ACAATTCACAACTTGTTACT+AGG + chr4.2:12609302-12609321 MS.gene08758:intron 30.0%
!!! TCTTTTTTTTGTGGTTCAGT+TGG + chr4.2:12608230-12608249 MS.gene08758:intron 30.0%
AAATTGGCTGATAACTGTAC+CGG + chr4.2:12608290-12608309 MS.gene08758:CDS 35.0%
ACTAGGACTTAATGTGATCT+GGG + chr4.2:12609319-12609338 MS.gene08758:intron 35.0%
ATCTTGAATGTTGTACCTGA+GGG - chr4.2:12608445-12608464 None:intergenic 35.0%
CAACTTATACCAACTTGAAC+AGG + chr4.2:12608732-12608751 MS.gene08758:CDS 35.0%
GAAATCTCGAAATTAGGGTT+AGG - chr4.2:12608188-12608207 None:intergenic 35.0%
GGAAATTGTAGATCTGTGTT+TGG + chr4.2:12608256-12608275 MS.gene08758:intron 35.0%
TACTAGGACTTAATGTGATC+TGG + chr4.2:12609318-12609337 MS.gene08758:intron 35.0%
TGATATCAACCTGTTCAAGT+TGG - chr4.2:12608744-12608763 None:intergenic 35.0%
TGATGTATAGATCATAGTGG+TGG + chr4.2:12608527-12608546 MS.gene08758:intron 35.0%
TGGGCTACTAATTTCTCTTA+TGG + chr4.2:12609338-12609357 MS.gene08758:intron 35.0%
TTTGGATCGTGTTAGGAAAT+TGG + chr4.2:12608274-12608293 MS.gene08758:CDS 35.0%
! ATACCACATTTGAAACCAGT+TGG - chr4.2:12609410-12609429 None:intergenic 35.0%
! CCGTAGCTGCATATTTTATA+AGG + chr4.2:12609182-12609201 MS.gene08758:intron 35.0%
! GAAAGCATGAAATGAATACG+AGG - chr4.2:12608857-12608876 None:intergenic 35.0%
! GAACGTCTTTCTGTTGATTA+TGG + chr4.2:12608383-12608402 MS.gene08758:CDS 35.0%
! TACCACATTTGAAACCAGTT+GGG - chr4.2:12609409-12609428 None:intergenic 35.0%
!! AACACAGCATTGGTAATTTC+CGG - chr4.2:12608935-12608954 None:intergenic 35.0%
!! CTGTTCAAGTTGGTATAAGT+TGG - chr4.2:12608734-12608753 None:intergenic 35.0%
!! TATAGATCATAGTGGTGGAT+TGG + chr4.2:12608532-12608551 MS.gene08758:intron 35.0%
!!! AACAGTCCTCTTTGTTTTGA+TGG - chr4.2:12609071-12609090 None:intergenic 35.0%
!!! TTTTTGGTTTTCAACGCTGT+TGG + chr4.2:12608323-12608342 MS.gene08758:CDS 35.0%
AACCAAAAACCCTTGAAGAC+CGG - chr4.2:12608312-12608331 None:intergenic 40.0%
AGGCGATCTATGACATTTGT+AGG + chr4.2:12608687-12608706 MS.gene08758:CDS 40.0%
ATCGAACTTGTGATCAATGC+GGG - chr4.2:12609534-12609553 None:intergenic 40.0%
ATCTGTGTTTGGATCGTGTT+AGG + chr4.2:12608267-12608286 MS.gene08758:CDS 40.0%
GATCGAACTTGTGATCAATG+CGG - chr4.2:12609535-12609554 None:intergenic 40.0%
GATCTTGAATGTTGTACCTG+AGG - chr4.2:12608446-12608465 None:intergenic 40.0%
GGAGATCTCTTGATATTGAG+AGG + chr4.2:12608708-12608727 MS.gene08758:CDS 40.0%
GTAGATGGTGAATCCAAGTT+TGG - chr4.2:12608417-12608436 None:intergenic 40.0%
GTCGATAGAACACTGTTGTA+TGG - chr4.2:12608620-12608639 None:intergenic 40.0%
TCCAAGGCATGGGAAATATA+TGG + chr4.2:12608985-12609004 MS.gene08758:CDS 40.0%
TCCTCTAGCGAAATTGTTAG+CGG - chr4.2:12608136-12608155 None:intergenic 40.0%
TCTTGAATGTTGTACCTGAG+GGG - chr4.2:12608444-12608463 None:intergenic 40.0%
TTGGCACCATCAAAACAAAG+AGG + chr4.2:12609062-12609081 MS.gene08758:CDS 40.0%
! ATAACTGTACCGGTCTTCAA+GGG + chr4.2:12608300-12608319 MS.gene08758:CDS 40.0%
! TTTTTGTGGTTCAGTTGGCA+AGG + chr4.2:12608235-12608254 MS.gene08758:intron 40.0%
!! AGGTTTGGGTTCACTTCTTT+TGG + chr4.2:12608361-12608380 MS.gene08758:CDS 40.0%
!! TATGAAGAAGTTGGTGCTGA+GGG + chr4.2:12609659-12609678 MS.gene08758:CDS 40.0%
!! TTATGAAGAAGTTGGTGCTG+AGG + chr4.2:12609658-12609677 MS.gene08758:CDS 40.0%
AAGGATGTCAATGCTGCTGT+TGG + chr4.2:12609043-12609062 MS.gene08758:CDS 45.0%
AAGTGAACCCAAACCTGAAC+CGG - chr4.2:12608357-12608376 None:intergenic 45.0%
AATGTTGTACCTGAGGGGAA+GGG - chr4.2:12608439-12608458 None:intergenic 45.0%
ACCTGTTATCTCTGCTGCTA+AGG + chr4.2:12608886-12608905 MS.gene08758:CDS 45.0%
AGAAGATGATGAGGAAGGAG+AGG + chr4.2:12609685-12609704 MS.gene08758:CDS 45.0%
CACTTAGCCATCATACTAGC+AGG - chr4.2:12608962-12608981 None:intergenic 45.0%
CAGTAACAACACTGCTGTTG+CGG + chr4.2:12609499-12609518 MS.gene08758:CDS 45.0%
CTAAGTGTGATCCAAGGCAT+GGG + chr4.2:12608975-12608994 MS.gene08758:CDS 45.0%
CTAGGTTTCAACTGCTGTTG+TGG + chr4.2:12608592-12608611 MS.gene08758:intron 45.0%
GCCATATATTTCCCATGCCT+TGG - chr4.2:12608989-12609008 None:intergenic 45.0%
GTTTGAGCCTGCTAGTATGA+TGG + chr4.2:12608952-12608971 MS.gene08758:CDS 45.0%
TCTTCTTGAGCACACTGATG+TGG + chr4.2:12608646-12608665 MS.gene08758:CDS 45.0%
TGATGGCTAAGTGTGATCCA+AGG + chr4.2:12608969-12608988 MS.gene08758:CDS 45.0%
! GATAACTGTACCGGTCTTCA+AGG + chr4.2:12608299-12608318 MS.gene08758:CDS 45.0%
! TACCGGTCTTCAAGGGTTTT+TGG + chr4.2:12608307-12608326 MS.gene08758:CDS 45.0%
! TGCCCAACTGGTTTCAAATG+TGG + chr4.2:12609404-12609423 MS.gene08758:CDS 45.0%
!! ACAGCAGCATTGACATCCTT+TGG - chr4.2:12609043-12609062 None:intergenic 45.0%
!! CCTTTGGAACAACATCACCA+CGG - chr4.2:12609027-12609046 None:intergenic 45.0%
!! GGACTGTTCAGTTTGTTGAC+TGG + chr4.2:12609083-12609102 MS.gene08758:CDS 45.0%
!! TTGGTTTTCAACGCTGTTGG+TGG + chr4.2:12608326-12608345 MS.gene08758:CDS 45.0%
AAACCTCAACGAGGTGGTGA+GGG - chr4.2:12608780-12608799 None:intergenic 50.0%
ACCACCTCGTTGAGGTTTGA+TGG + chr4.2:12608782-12608801 MS.gene08758:CDS 50.0%
ACCATCAAACCTCAACGAGG+TGG - chr4.2:12608786-12608805 None:intergenic 50.0%
ACCGACATACCAGTGGACAA+AGG - chr4.2:12609576-12609595 None:intergenic 50.0%
ACGAGGATACGGCACAAGAT+TGG - chr4.2:12608840-12608859 None:intergenic 50.0%
AGCACCATCAAACCTCAACG+AGG - chr4.2:12608789-12608808 None:intergenic 50.0%
AGCAGGCTCAAACACAGCAT+TGG - chr4.2:12608945-12608964 None:intergenic 50.0%
ATGTATGACAGGTGCCCAAC+TGG + chr4.2:12609392-12609411 MS.gene08758:intron 50.0%
CACCCTGAACAACTCATCTC+CGG + chr4.2:12608099-12608118 MS.gene08758:CDS 50.0%
CAGCTCTTTGCACCTTAGCA+AGG - chr4.2:12609469-12609488 None:intergenic 50.0%
CGAGAGCAGCAAGATCTTCA+CGG - chr4.2:12609631-12609650 None:intergenic 50.0%
GAAGAGTTGTCGGTAGGTAC+CGG - chr4.2:12608079-12608098 None:intergenic 50.0%
GAATGTTGTACCTGAGGGGA+AGG - chr4.2:12608440-12608459 None:intergenic 50.0%
GAGAGCAGCAAGATCTTCAC+GGG - chr4.2:12609630-12609649 None:intergenic 50.0%
GCCGCTAACAATTTCGCTAG+AGG + chr4.2:12608132-12608151 MS.gene08758:CDS 50.0%
GCCTTAGCAGCAGAGATAAC+AGG - chr4.2:12608890-12608909 None:intergenic 50.0%
GCCTTTGTCCACTGGTATGT+CGG + chr4.2:12609572-12609591 MS.gene08758:CDS 50.0%
GCTAAGTGTGATCCAAGGCA+TGG + chr4.2:12608974-12608993 MS.gene08758:CDS 50.0%
GGAGCAGAAGATGATGAGGA+AGG + chr4.2:12609680-12609699 MS.gene08758:CDS 50.0%
GTTCAGGGTGGAAGAGTTGT+CGG - chr4.2:12608089-12608108 None:intergenic 50.0%
TAACAACACTGCTGTTGCGG+AGG + chr4.2:12609502-12609521 MS.gene08758:CDS 50.0%
TCATGAGCAGCTATCTGTGC+CGG + chr4.2:12608913-12608932 MS.gene08758:CDS 50.0%
TCTTGAGCACACTGATGTGG+CGG + chr4.2:12608649-12608668 MS.gene08758:CDS 50.0%
TGAGGGAGCAGAAGATGATG+AGG + chr4.2:12609676-12609695 MS.gene08758:CDS 50.0%
TGCCGGAGATGAGTTGTTCA+GGG - chr4.2:12608104-12608123 None:intergenic 50.0%
TTCGCTAGAGGACACTACAC+TGG + chr4.2:12608144-12608163 MS.gene08758:CDS 50.0%
TTGCCGGAGATGAGTTGTTC+AGG - chr4.2:12608105-12608124 None:intergenic 50.0%
! CCGTGGTGATGTTGTTCCAA+AGG + chr4.2:12609024-12609043 MS.gene08758:CDS 50.0%
! GATACGGCACAAGATTGGTC+TGG - chr4.2:12608835-12608854 None:intergenic 50.0%
!! GCTTGCTGTTTGATGTACCG+TGG + chr4.2:12609007-12609026 MS.gene08758:CDS 50.0%
!! GTTTTCAACGCTGTTGGTGG+TGG + chr4.2:12608329-12608348 MS.gene08758:CDS 50.0%
AGGGTGGAAGAGTTGTCGGT+AGG - chr4.2:12608085-12608104 None:intergenic 55.0%
CAAACCTCAACGAGGTGGTG+AGG - chr4.2:12608781-12608800 None:intergenic 55.0%
CAGCCTCCTTCAGTTGTTCC+TGG + chr4.2:12609437-12609456 MS.gene08758:CDS 55.0%
CCAAGAGAGCCTTTGTCCAC+TGG + chr4.2:12609564-12609583 MS.gene08758:CDS 55.0%
CCAGTGGACAAAGGCTCTCT+TGG - chr4.2:12609567-12609586 None:intergenic 55.0%
CCTCCTTCAGTTGTTCCTGG+CGG + chr4.2:12609440-12609459 MS.gene08758:CDS 55.0%
CGGAGATGAGTTGTTCAGGG+TGG - chr4.2:12608101-12608120 None:intergenic 55.0%
GGCGGTGCTCTTGGATAATG+AGG + chr4.2:12608667-12608686 MS.gene08758:CDS 55.0%
GTCCACTGGTATGTCGGTGA+GGG + chr4.2:12609578-12609597 MS.gene08758:CDS 55.0%
GTTAGCGGCGTCTTCTTTGC+CGG - chr4.2:12608121-12608140 None:intergenic 55.0%
TACCTGAGGGGAAGGGTAGA+TGG - chr4.2:12608432-12608451 None:intergenic 55.0%
TCACCGCCAGGAACAACTGA+AGG - chr4.2:12609446-12609465 None:intergenic 55.0%
TGTCCACTGGTATGTCGGTG+AGG + chr4.2:12609577-12609596 MS.gene08758:CDS 55.0%
! GGTGGAACCGGTTCAGGTTT+GGG + chr4.2:12608347-12608366 MS.gene08758:CDS 55.0%
! TCGGTGAGGGCATGGAAGAA+GGG + chr4.2:12609591-12609610 MS.gene08758:CDS 55.0%
! TGGTGGAACCGGTTCAGGTT+TGG + chr4.2:12608346-12608365 MS.gene08758:CDS 55.0%
!! AACGCTGTTGGTGGTGGAAC+CGG + chr4.2:12608335-12608354 MS.gene08758:CDS 55.0%
ACCTTAGCAAGGTCACCGCC+AGG - chr4.2:12609458-12609477 None:intergenic 60.0%
CACCATCTACCCTTCCCCTC+AGG + chr4.2:12608427-12608446 MS.gene08758:CDS 60.0%
CACTGATGTGGCGGTGCTCT+TGG + chr4.2:12608658-12608677 MS.gene08758:CDS 60.0%
CATCCCTCACCACCTCGTTG+AGG + chr4.2:12608774-12608793 MS.gene08758:CDS 60.0%
CCGCCAGGAACAACTGAAGG+AGG - chr4.2:12609443-12609462 None:intergenic 60.0%
CTGGTATGTCGGTGAGGGCA+TGG + chr4.2:12609583-12609602 MS.gene08758:CDS 60.0%
TCCTGGCGGTGACCTTGCTA+AGG + chr4.2:12609454-12609473 MS.gene08758:CDS 60.0%
TGCCCTCACCGACATACCAG+TGG - chr4.2:12609583-12609602 None:intergenic 60.0%
! CGGTGAGGGCATGGAAGAAG+GGG + chr4.2:12609592-12609611 MS.gene08758:CDS 60.0%
! GTCGGTGAGGGCATGGAAGA+AGG + chr4.2:12609590-12609609 MS.gene08758:CDS 60.0%
!! GTTGGTGGTGGAACCGGTTC+AGG + chr4.2:12608341-12608360 MS.gene08758:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 12608064 12609715 12608064 ID=MS.gene08758
chr4.2 mRNA 12608064 12609715 12608064 ID=MS.gene08758.t1;Parent=MS.gene08758
chr4.2 exon 12608064 12608165 12608064 ID=MS.gene08758.t1.exon1;Parent=MS.gene08758.t1
chr4.2 CDS 12608064 12608165 12608064 ID=cds.MS.gene08758.t1;Parent=MS.gene08758.t1
chr4.2 exon 12608257 12608448 12608257 ID=MS.gene08758.t1.exon2;Parent=MS.gene08758.t1
chr4.2 CDS 12608257 12608448 12608257 ID=cds.MS.gene08758.t1;Parent=MS.gene08758.t1
chr4.2 exon 12608596 12609104 12608596 ID=MS.gene08758.t1.exon3;Parent=MS.gene08758.t1
chr4.2 CDS 12608596 12609104 12608596 ID=cds.MS.gene08758.t1;Parent=MS.gene08758.t1
chr4.2 exon 12609403 12609715 12609403 ID=MS.gene08758.t1.exon4;Parent=MS.gene08758.t1
chr4.2 CDS 12609403 12609715 12609403 ID=cds.MS.gene08758.t1;Parent=MS.gene08758.t1
Gene Sequence

>MS.gene08758

ATGAAGTTCGTTCCGGTACCTACCGACAACTCTTCCACCCTGAACAACTCATCTCCGGCAAAGAAGACGCCGCTAACAATTTCGCTAGAGGACACTACACTGGTCAGTTTCTTTTTCAAACCCTAACCCTAATTTCGAGATTTCTTGCTTTTTATTGATTTTTTGTTCTTTTTTTTGTGGTTCAGTTGGCAAGGAAATTGTAGATCTGTGTTTGGATCGTGTTAGGAAATTGGCTGATAACTGTACCGGTCTTCAAGGGTTTTTGGTTTTCAACGCTGTTGGTGGTGGAACCGGTTCAGGTTTGGGTTCACTTCTTTTGGAACGTCTTTCTGTTGATTATGGAAAGAAATCCAAACTTGGATTCACCATCTACCCTTCCCCTCAGGTACAACATTCAAGATCTTTAATACTGTTATTGTTATTGCAATGTTTAAATCATGTTGATGTAAATATTGGTGTTGATTGATGTATAGATCATAGTGGTGGATTGGTTTTTCTGTTTGTAATGGTGAATTGTTATTTTTGGATCTAGGTTTCAACTGCTGTTGTGGAGCCATACAACAGTGTTCTATCGACACATTCTCTTCTTGAGCACACTGATGTGGCGGTGCTCTTGGATAATGAGGCGATCTATGACATTTGTAGGAGATCTCTTGATATTGAGAGGCCAACTTATACCAACTTGAACAGGTTGATATCACAGATCATATCATCCCTCACCACCTCGTTGAGGTTTGATGGTGCTATCAATGTTGATATTACTGAGTTCCAGACCAATCTTGTGCCGTATCCTCGTATTCATTTCATGCTTTCGTCATATGCACCTGTTATCTCTGCTGCTAAGGCTTATCATGAGCAGCTATCTGTGCCGGAAATTACCAATGCTGTGTTTGAGCCTGCTAGTATGATGGCTAAGTGTGATCCAAGGCATGGGAAATATATGGCTTGCTGTTTGATGTACCGTGGTGATGTTGTTCCAAAGGATGTCAATGCTGCTGTTGGCACCATCAAAACAAAGAGGACTGTTCAGTTTGTTGACTGGTAACGACTCTTTCAATAACTATTTGTAGAATGACGGTGTTGTCTCGCATTGCATGTTCTGATCTGTTTAGAATTATCCGTAGCTGCATATTTTATAAGGGTAGATAATTTGGAAGATAGCATAATAATTAAGAGCTTGAAAAACTATAGCAACTAATAATACTGATTTGAAATAATCAAATTTTTTTTCTTTAGCAACAATTCACAACTTGTTACTAGGACTTAATGTGATCTGGGCTACTAATTTCTCTTATGGTGATATTCTCTATAACATTGTTCATATTATAATGTATGACAGGTGCCCAACTGGTTTCAAATGTGGTATCAACTATCAGCCTCCTTCAGTTGTTCCTGGCGGTGACCTTGCTAAGGTGCAAAGAGCTGTGTGTATGATCAGTAACAACACTGCTGTTGCGGAGGTCTTCTCCCGCATTGATCACAAGTTCGATCTGATGTATGCCAAGAGAGCCTTTGTCCACTGGTATGTCGGTGAGGGCATGGAAGAAGGGGAGTTCTCAGAAGCCCGTGAAGATCTTGCTGCTCTCGAGAAAGATTATGAAGAAGTTGGTGCTGAGGGAGCAGAAGATGATGAGGAAGGAGAGGATTATTGA
Protein sequence

>MS.gene08758.t1

MKFVPVPTDNSSTLNNSSPAKKTPLTISLEDTTLEIVDLCLDRVRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTIYPSPQVSTAVVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPTYTNLNRLISQIISSLTTSLRFDGAINVDITEFQTNLVPYPRIHFMLSSYAPVISAAKAYHEQLSVPEITNAVFEPASMMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVGTIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNNTAVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEGAEDDEEGEDY