Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058492.t1 | RHN42672.1 | 97.2 | 466 | 13 | 0 | 1 | 466 | 14 | 479 | 1.00E-248 | 869 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058492.t1 | F4KGE8 | 29.1 | 406 | 252 | 7 | 79 | 458 | 160 | 555 | 1.5e-32 | 142.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058492.t1 | G7L787 | 97.2 | 466 | 13 | 0 | 1 | 466 | 1 | 466 | 7.2e-249 | 869.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049350 | MS.gene058492 | 0.808169 | 3.53E-50 | -1.69E-46 |
| MS.gene049711 | MS.gene058492 | 0.801496 | 8.76E-49 | -1.69E-46 |
| MS.gene049807 | MS.gene058492 | 0.850485 | 1.65E-60 | -1.69E-46 |
| MS.gene051234 | MS.gene058492 | 0.811858 | 5.67E-51 | -1.69E-46 |
| MS.gene052335 | MS.gene058492 | 0.80047 | 1.42E-48 | -1.69E-46 |
| MS.gene053143 | MS.gene058492 | 0.816295 | 5.95E-52 | -1.69E-46 |
| MS.gene053144 | MS.gene058492 | 0.804749 | 1.86E-49 | -1.69E-46 |
| MS.gene053171 | MS.gene058492 | 0.823906 | 1.08E-53 | -1.69E-46 |
| MS.gene054554 | MS.gene058492 | 0.818041 | 2.41E-52 | -1.69E-46 |
| MS.gene054584 | MS.gene058492 | 0.801545 | 8.56E-49 | -1.69E-46 |
| MS.gene055469 | MS.gene058492 | 0.803676 | 3.11E-49 | -1.69E-46 |
| MS.gene055537 | MS.gene058492 | 0.822484 | 2.31E-53 | -1.69E-46 |
| MS.gene057102 | MS.gene058492 | 0.811149 | 8.09E-51 | -1.69E-46 |
| MS.gene058492 | MS.gene058852 | 0.806209 | 9.19E-50 | -1.69E-46 |
| MS.gene058492 | MS.gene059778 | 0.805039 | 1.62E-49 | -1.69E-46 |
| MS.gene058492 | MS.gene061100 | 0.828109 | 1.08E-54 | -1.69E-46 |
| MS.gene058492 | MS.gene061564 | 0.812097 | 5.03E-51 | -1.69E-46 |
| MS.gene058492 | MS.gene061707 | 0.838054 | 3.61E-57 | -1.69E-46 |
| MS.gene058492 | MS.gene06258 | 0.848971 | 4.36E-60 | -1.69E-46 |
| MS.gene058492 | MS.gene063667 | 0.801788 | 7.63E-49 | -1.69E-46 |
| MS.gene058492 | MS.gene064243 | 0.818388 | 2.01E-52 | -1.69E-46 |
| MS.gene058492 | MS.gene065055 | 0.839975 | 1.15E-57 | -1.69E-46 |
| MS.gene058492 | MS.gene066196 | 0.84357 | 1.29E-58 | -1.69E-46 |
| MS.gene058492 | MS.gene068022 | 0.801537 | 8.59E-49 | -1.69E-46 |
| MS.gene058492 | MS.gene068925 | 0.8041 | 2.54E-49 | -1.69E-46 |
| MS.gene058492 | MS.gene070339 | 0.813822 | 2.11E-51 | -1.69E-46 |
| MS.gene058492 | MS.gene071974 | 0.801394 | 9.19E-49 | -1.69E-46 |
| MS.gene058492 | MS.gene072116 | 0.813688 | 2.26E-51 | -1.69E-46 |
| MS.gene058492 | MS.gene073262 | 0.814823 | 1.27E-51 | -1.69E-46 |
| MS.gene058492 | MS.gene07374 | 0.801878 | 7.31E-49 | -1.69E-46 |
| MS.gene058492 | MS.gene074317 | 0.803096 | 4.10E-49 | -1.69E-46 |
| MS.gene058492 | MS.gene074519 | 0.83313 | 6.36E-56 | -1.69E-46 |
| MS.gene058492 | MS.gene08758 | 0.802489 | 5.48E-49 | -1.69E-46 |
| MS.gene058492 | MS.gene08826 | 0.808951 | 2.41E-50 | -1.69E-46 |
| MS.gene058492 | MS.gene08980 | 0.808934 | 2.43E-50 | -1.69E-46 |
| MS.gene058492 | MS.gene09934 | 0.812333 | 4.47E-51 | -1.69E-46 |
| MS.gene058492 | MS.gene20061 | 0.812125 | 4.96E-51 | -1.69E-46 |
| MS.gene058492 | MS.gene20457 | 0.840282 | 9.55E-58 | -1.69E-46 |
| MS.gene058492 | MS.gene20557 | 0.808866 | 2.51E-50 | -1.69E-46 |
| MS.gene058492 | MS.gene22553 | 0.817084 | 3.96E-52 | -1.69E-46 |
| MS.gene058492 | MS.gene24076 | 0.839545 | 1.49E-57 | -1.69E-46 |
| MS.gene058492 | MS.gene24078 | 0.810063 | 1.39E-50 | -1.69E-46 |
| MS.gene058492 | MS.gene24330 | 0.80027 | 1.56E-48 | -1.69E-46 |
| MS.gene058492 | MS.gene25981 | -0.823991 | 1.03E-53 | -1.69E-46 |
| MS.gene058492 | MS.gene26380 | 0.826858 | 2.15E-54 | -1.69E-46 |
| MS.gene058492 | MS.gene26387 | 0.80463 | 1.97E-49 | -1.69E-46 |
| MS.gene058492 | MS.gene26659 | 0.812024 | 5.22E-51 | -1.69E-46 |
| MS.gene058492 | MS.gene26851 | 0.807999 | 3.84E-50 | -1.69E-46 |
| MS.gene058492 | MS.gene26945 | 0.813705 | 2.24E-51 | -1.69E-46 |
| MS.gene058492 | MS.gene27646 | -0.83532 | 1.80E-56 | -1.69E-46 |
| MS.gene058492 | MS.gene27831 | 0.996975 | 5.69E-235 | -1.69E-46 |
| MS.gene058492 | MS.gene28611 | 0.827522 | 1.49E-54 | -1.69E-46 |
| MS.gene058492 | MS.gene28813 | 0.825178 | 5.40E-54 | -1.69E-46 |
| MS.gene058492 | MS.gene28979 | 0.844295 | 8.26E-59 | -1.69E-46 |
| MS.gene058492 | MS.gene29355 | 0.822815 | 1.94E-53 | -1.69E-46 |
| MS.gene058492 | MS.gene31336 | 0.805209 | 1.49E-49 | -1.69E-46 |
| MS.gene058492 | MS.gene31571 | 0.803632 | 3.18E-49 | -1.69E-46 |
| MS.gene058492 | MS.gene32653 | 0.818084 | 2.36E-52 | -1.69E-46 |
| MS.gene058492 | MS.gene32688 | 0.816601 | 5.09E-52 | -1.69E-46 |
| MS.gene058492 | MS.gene34164 | 0.816245 | 6.11E-52 | -1.69E-46 |
| MS.gene058492 | MS.gene34266 | 0.818663 | 1.74E-52 | -1.69E-46 |
| MS.gene058492 | MS.gene34950 | 0.814548 | 1.46E-51 | -1.69E-46 |
| MS.gene058492 | MS.gene36285 | 0.817811 | 2.72E-52 | -1.69E-46 |
| MS.gene058492 | MS.gene37106 | 0.839977 | 1.15E-57 | -1.69E-46 |
| MS.gene058492 | MS.gene38303 | 0.806469 | 8.10E-50 | -1.69E-46 |
| MS.gene058492 | MS.gene39046 | 0.823219 | 1.56E-53 | -1.69E-46 |
| MS.gene058492 | MS.gene39052 | 0.813324 | 2.71E-51 | -1.69E-46 |
| MS.gene058492 | MS.gene40787 | 0.804549 | 2.05E-49 | -1.69E-46 |
| MS.gene058492 | MS.gene41651 | 0.851065 | 1.13E-60 | -1.69E-46 |
| MS.gene058492 | MS.gene42377 | 0.802459 | 5.55E-49 | -1.69E-46 |
| MS.gene058492 | MS.gene42653 | 0.818912 | 1.53E-52 | -1.69E-46 |
| MS.gene058492 | MS.gene45281 | 0.826426 | 2.73E-54 | -1.69E-46 |
| MS.gene058492 | MS.gene45425 | 0.823 | 1.75E-53 | -1.69E-46 |
| MS.gene058492 | MS.gene45857 | 0.810698 | 1.01E-50 | -1.69E-46 |
| MS.gene058492 | MS.gene46048 | 0.80037 | 1.49E-48 | -1.69E-46 |
| MS.gene058492 | MS.gene46127 | 0.807359 | 5.25E-50 | -1.69E-46 |
| MS.gene058492 | MS.gene47768 | 0.810616 | 1.06E-50 | -1.69E-46 |
| MS.gene058492 | MS.gene50004 | 0.810768 | 9.78E-51 | -1.69E-46 |
| MS.gene058492 | MS.gene50013 | 0.807842 | 4.15E-50 | -1.69E-46 |
| MS.gene058492 | MS.gene51365 | 0.808271 | 3.36E-50 | -1.69E-46 |
| MS.gene058492 | MS.gene51384 | 0.808446 | 3.08E-50 | -1.69E-46 |
| MS.gene058492 | MS.gene53933 | 0.809472 | 1.86E-50 | -1.69E-46 |
| MS.gene058492 | MS.gene55101 | 0.801884 | 7.29E-49 | -1.69E-46 |
| MS.gene058492 | MS.gene55623 | 0.82506 | 5.76E-54 | -1.69E-46 |
| MS.gene058492 | MS.gene55904 | 0.849801 | 2.56E-60 | -1.69E-46 |
| MS.gene058492 | MS.gene56169 | 0.818971 | 1.48E-52 | -1.69E-46 |
| MS.gene058492 | MS.gene56476 | 0.814382 | 1.59E-51 | -1.69E-46 |
| MS.gene058492 | MS.gene56510 | 0.834343 | 3.17E-56 | -1.69E-46 |
| MS.gene058492 | MS.gene56565 | 0.838369 | 3.00E-57 | -1.69E-46 |
| MS.gene058492 | MS.gene57576 | 0.820391 | 7.03E-53 | -1.69E-46 |
| MS.gene058492 | MS.gene58122 | 0.819304 | 1.25E-52 | -1.69E-46 |
| MS.gene058492 | MS.gene58719 | 0.812027 | 5.21E-51 | -1.69E-46 |
| MS.gene058492 | MS.gene58836 | 0.800735 | 1.25E-48 | -1.69E-46 |
| MS.gene058492 | MS.gene59241 | 0.870914 | 1.02E-66 | -1.69E-46 |
| MS.gene058492 | MS.gene59242 | 0.869401 | 3.18E-66 | -1.69E-46 |
| MS.gene058492 | MS.gene59775 | 0.806307 | 8.77E-50 | -1.69E-46 |
| MS.gene058492 | MS.gene59860 | 0.80534 | 1.40E-49 | -1.69E-46 |
| MS.gene058492 | MS.gene61739 | 0.800314 | 1.53E-48 | -1.69E-46 |
| MS.gene058492 | MS.gene62423 | 0.853848 | 1.82E-61 | -1.69E-46 |
| MS.gene058492 | MS.gene62916 | 0.801699 | 7.96E-49 | -1.69E-46 |
| MS.gene058492 | MS.gene65433 | 0.826083 | 3.29E-54 | -1.69E-46 |
| MS.gene058492 | MS.gene66156 | 0.811766 | 5.94E-51 | -1.69E-46 |
| MS.gene058492 | MS.gene66409 | 0.800322 | 1.52E-48 | -1.69E-46 |
| MS.gene058492 | MS.gene66496 | 0.82009 | 8.24E-53 | -1.69E-46 |
| MS.gene058492 | MS.gene66786 | 0.831681 | 1.45E-55 | -1.69E-46 |
| MS.gene058492 | MS.gene66801 | 0.800617 | 1.33E-48 | -1.69E-46 |
| MS.gene058492 | MS.gene66845 | 0.854926 | 8.89E-62 | -1.69E-46 |
| MS.gene058492 | MS.gene67881 | 0.83585 | 1.32E-56 | -1.69E-46 |
| MS.gene058492 | MS.gene68106 | 0.827854 | 1.24E-54 | -1.69E-46 |
| MS.gene058492 | MS.gene68418 | 0.815877 | 7.38E-52 | -1.69E-46 |
| MS.gene058492 | MS.gene68420 | 0.820034 | 8.49E-53 | -1.69E-46 |
| MS.gene058492 | MS.gene68663 | 0.80448 | 2.12E-49 | -1.69E-46 |
| MS.gene058492 | MS.gene69313 | 0.8297 | 4.45E-55 | -1.69E-46 |
| MS.gene058492 | MS.gene69823 | 0.8019 | 7.24E-49 | -1.69E-46 |
| MS.gene058492 | MS.gene72170 | 0.810515 | 1.11E-50 | -1.69E-46 |
| MS.gene058492 | MS.gene72487 | 0.822081 | 2.87E-53 | -1.69E-46 |
| MS.gene058492 | MS.gene74606 | 0.855587 | 5.71E-62 | -1.69E-46 |
| MS.gene058492 | MS.gene74972 | 0.831025 | 2.11E-55 | -1.69E-46 |
| MS.gene058492 | MS.gene75719 | 0.816953 | 4.24E-52 | -1.69E-46 |
| MS.gene058492 | MS.gene76227 | 0.835107 | 2.03E-56 | -1.69E-46 |
| MS.gene058492 | MS.gene76314 | 0.857512 | 1.55E-62 | -1.69E-46 |
| MS.gene058492 | MS.gene78213 | 0.803222 | 3.86E-49 | -1.69E-46 |
| MS.gene058492 | MS.gene79607 | 0.80082 | 1.20E-48 | -1.69E-46 |
| MS.gene058492 | MS.gene80201 | 0.817102 | 3.92E-52 | -1.69E-46 |
| MS.gene058492 | MS.gene82912 | 0.809689 | 1.67E-50 | -1.69E-46 |
| MS.gene058492 | MS.gene82993 | 0.807354 | 5.27E-50 | -1.69E-46 |
| MS.gene058492 | MS.gene83122 | 0.805955 | 1.04E-49 | -1.69E-46 |
| MS.gene058492 | MS.gene84298 | 0.805263 | 1.45E-49 | -1.69E-46 |
| MS.gene058492 | MS.gene88592 | 0.811327 | 7.40E-51 | -1.69E-46 |
| MS.gene058492 | MS.gene88927 | 0.826174 | 3.13E-54 | -1.69E-46 |
| MS.gene058492 | MS.gene89100 | 0.824661 | 7.15E-54 | -1.69E-46 |
| MS.gene058492 | MS.gene89248 | 0.81976 | 9.81E-53 | -1.69E-46 |
| MS.gene058492 | MS.gene89812 | 0.811369 | 7.25E-51 | -1.69E-46 |
| MS.gene058492 | MS.gene89985 | 0.80967 | 1.69E-50 | -1.69E-46 |
| MS.gene058492 | MS.gene90381 | 0.818608 | 1.79E-52 | -1.69E-46 |
| MS.gene058492 | MS.gene90880 | 0.841756 | 3.92E-58 | -1.69E-46 |
| MS.gene058492 | MS.gene91114 | 0.813731 | 2.21E-51 | -1.69E-46 |
| MS.gene058492 | MS.gene92342 | 0.806845 | 6.75E-50 | -1.69E-46 |
| MS.gene058492 | MS.gene96190 | 0.813575 | 2.39E-51 | -1.69E-46 |
| MS.gene058492 | MS.gene99949 | 0.815182 | 1.05E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene59941 | MS.gene058492 | PPI |
| MS.gene032321 | MS.gene058492 | PPI |
| MS.gene058492 | MS.gene48940 | PPI |
| MS.gene32238 | MS.gene058492 | PPI |
| MS.gene060213 | MS.gene058492 | PPI |
| MS.gene48223 | MS.gene058492 | PPI |
| MS.gene38998 | MS.gene058492 | PPI |
| MS.gene45832 | MS.gene058492 | PPI |
| MS.gene058492 | MS.gene63724 | PPI |
| MS.gene007857 | MS.gene058492 | PPI |
| MS.gene059205 | MS.gene058492 | PPI |
| MS.gene026867 | MS.gene058492 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058492.t1 | MTR_8g087400 | 97.639 | 466 | 11 | 0 | 1 | 466 | 1 | 466 | 0.0 | 942 |
| MS.gene058492.t1 | MTR_2g075910 | 44.372 | 462 | 244 | 6 | 1 | 458 | 1 | 453 | 1.42e-123 | 369 |
| MS.gene058492.t1 | MTR_8g059485 | 43.656 | 465 | 228 | 9 | 1 | 459 | 1 | 437 | 2.46e-119 | 357 |
| MS.gene058492.t1 | MTR_8g059485 | 43.656 | 465 | 228 | 9 | 1 | 459 | 10 | 446 | 2.75e-119 | 357 |
| MS.gene058492.t1 | MTR_4g025420 | 38.750 | 400 | 215 | 5 | 74 | 459 | 30 | 413 | 5.99e-94 | 290 |
| MS.gene058492.t1 | MTR_0178s0030 | 33.414 | 413 | 246 | 6 | 62 | 459 | 125 | 523 | 3.34e-63 | 214 |
| MS.gene058492.t1 | MTR_3g462150 | 28.715 | 397 | 256 | 5 | 79 | 458 | 91 | 477 | 7.01e-50 | 177 |
| MS.gene058492.t1 | MTR_5g075180 | 30.316 | 475 | 280 | 12 | 4 | 465 | 10 | 446 | 3.05e-49 | 175 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058492.t1 | AT1G29300 | 54.054 | 481 | 184 | 9 | 1 | 466 | 1 | 459 | 2.30e-169 | 484 |
| MS.gene058492.t1 | AT3G14870 | 45.064 | 466 | 245 | 7 | 1 | 459 | 5 | 466 | 4.80e-132 | 390 |
| MS.gene058492.t1 | AT3G14870 | 45.064 | 466 | 245 | 7 | 1 | 459 | 1 | 462 | 5.02e-132 | 390 |
| MS.gene058492.t1 | AT3G14870 | 45.064 | 466 | 245 | 7 | 1 | 459 | 1 | 462 | 5.02e-132 | 390 |
| MS.gene058492.t1 | AT3G14870 | 45.064 | 466 | 245 | 7 | 1 | 459 | 1 | 462 | 5.02e-132 | 390 |
| MS.gene058492.t1 | AT3G14870 | 45.064 | 466 | 245 | 7 | 1 | 459 | 2 | 463 | 5.24e-132 | 390 |
| MS.gene058492.t1 | AT1G53380 | 44.017 | 468 | 238 | 5 | 1 | 460 | 1 | 452 | 3.15e-130 | 385 |
| MS.gene058492.t1 | AT1G53380 | 44.017 | 468 | 238 | 5 | 1 | 460 | 1 | 452 | 3.15e-130 | 385 |
| MS.gene058492.t1 | AT1G53380 | 44.017 | 468 | 238 | 5 | 1 | 460 | 1 | 452 | 3.15e-130 | 385 |
| MS.gene058492.t1 | AT1G53380 | 44.017 | 468 | 238 | 5 | 1 | 460 | 1 | 452 | 3.15e-130 | 385 |
| MS.gene058492.t1 | AT1G53380 | 44.017 | 468 | 238 | 5 | 1 | 460 | 1 | 452 | 3.15e-130 | 385 |
| MS.gene058492.t1 | AT2G45260 | 40.100 | 399 | 220 | 4 | 74 | 461 | 31 | 421 | 9.76e-97 | 298 |
| MS.gene058492.t1 | AT3G60680 | 34.367 | 387 | 234 | 5 | 81 | 459 | 120 | 494 | 4.10e-72 | 236 |
| MS.gene058492.t1 | AT2G30380 | 32.663 | 398 | 217 | 8 | 67 | 459 | 60 | 411 | 1.12e-55 | 191 |
| MS.gene058492.t1 | AT4G34080 | 36.364 | 275 | 164 | 3 | 74 | 337 | 7 | 281 | 1.59e-53 | 181 |
| MS.gene058492.t1 | AT4G33320 | 35.448 | 268 | 161 | 4 | 74 | 329 | 7 | 274 | 3.21e-48 | 167 |
| MS.gene058492.t1 | AT5G58960 | 29.557 | 406 | 250 | 7 | 79 | 458 | 85 | 480 | 5.69e-44 | 161 |
| MS.gene058492.t1 | AT5G58960 | 29.557 | 406 | 250 | 7 | 79 | 458 | 85 | 480 | 5.69e-44 | 161 |
| MS.gene058492.t1 | AT5G58960 | 29.557 | 406 | 250 | 7 | 79 | 458 | 160 | 555 | 1.75e-43 | 161 |
| MS.gene058492.t1 | AT2G32130 | 57.724 | 123 | 52 | 0 | 80 | 202 | 34 | 156 | 6.62e-43 | 149 |
| MS.gene058492.t1 | AT4G36100 | 43.636 | 110 | 57 | 2 | 74 | 178 | 7 | 116 | 7.08e-21 | 91.7 |
Find 58 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTTGTTCATTGCTGATTT+TGG | 0.079747 | 4.1:-13308502 | None:intergenic |
| TTCTAATTCCGGCGAGTTTC+GGG | 0.120775 | 4.1:+13309773 | MS.gene058492:CDS |
| ATGGACAACTTGATGATATT+TGG | 0.163563 | 4.1:-13309493 | None:intergenic |
| ATTCTAATTCCGGCGAGTTT+CGG | 0.208130 | 4.1:+13309772 | MS.gene058492:CDS |
| TCATTTGAAACAGTGAAATT+TGG | 0.218352 | 4.1:-13309351 | None:intergenic |
| ATTTGTCAAAATAGTGATTT+TGG | 0.234573 | 4.1:-13309392 | None:intergenic |
| GATTCTATTGATTCTAATTC+CGG | 0.300156 | 4.1:+13309762 | MS.gene058492:CDS |
| CAGAGGAAGTTAGTGAATTC+TGG | 0.306974 | 4.1:+13309552 | MS.gene058492:CDS |
| TTCTCCTTGAAGCTTCTTGA+TGG | 0.321429 | 4.1:-13308977 | None:intergenic |
| CTTCTTGATGGTGATTTCAT+AGG | 0.327494 | 4.1:-13308965 | None:intergenic |
| TTCTTATTGATCTCAAAGTA+TGG | 0.329293 | 4.1:-13309167 | None:intergenic |
| GAGTCAGGTTTATCTGTCTT+TGG | 0.330222 | 4.1:+13309845 | MS.gene058492:CDS |
| CTGAGATGGCAAAGCGTGTT+TGG | 0.331303 | 4.1:+13309610 | MS.gene058492:CDS |
| TCAAGTTGTCCATGCTAAAA+TGG | 0.340049 | 4.1:+13309503 | MS.gene058492:CDS |
| GAAACTTATGATTGAAGAAA+TGG | 0.349019 | 4.1:+13309197 | MS.gene058492:CDS |
| TCAGTTTCAACTTCATGTTC+AGG | 0.353607 | 4.1:+13309738 | MS.gene058492:CDS |
| AAAATGGAGTGTTCTTTGTT+TGG | 0.368125 | 4.1:+13309519 | MS.gene058492:CDS |
| AAGAAATGGAATCAGCAAAC+TGG | 0.369176 | 4.1:+13309211 | MS.gene058492:CDS |
| GATTCAACATTGAAAGTTCA+AGG | 0.402308 | 4.1:-13309119 | None:intergenic |
| AACTTGGCAAAAGATGAGTT+TGG | 0.414833 | 4.1:-13309462 | None:intergenic |
| TTCTAACCTTGTTCTTCTGT+TGG | 0.416097 | 4.1:-13308687 | None:intergenic |
| GGTTAGAAACAAAGCAGTTA+TGG | 0.418035 | 4.1:+13308702 | MS.gene058492:CDS |
| CAGAATTCACTAACTTCCTC+TGG | 0.439916 | 4.1:-13309551 | None:intergenic |
| TGTTTGGACACTGCATTACT+TGG | 0.442751 | 4.1:+13309626 | MS.gene058492:CDS |
| AAAACAACGATGACTTGGTT+TGG | 0.448235 | 4.1:-13309280 | None:intergenic |
| ATCATATGCAGAACTTCAAA+TGG | 0.451722 | 4.1:+13308771 | MS.gene058492:CDS |
| TAGGTTCTCTGAGGTTTACA+TGG | 0.451972 | 4.1:+13309698 | MS.gene058492:CDS |
| ATCACCATCAAGAAGCTTCA+AGG | 0.452355 | 4.1:+13308973 | MS.gene058492:CDS |
| CAGTTTCAACTTCATGTTCA+GGG | 0.460825 | 4.1:+13309739 | MS.gene058492:CDS |
| TGTATTGAATCATTGTTGTA+AGG | 0.470099 | 4.1:-13308802 | None:intergenic |
| TAAGACAGTGATACAGAGTC+AGG | 0.472174 | 4.1:+13309830 | MS.gene058492:CDS |
| TCAGGTTTATCTGTCTTTGG+TGG | 0.472612 | 4.1:+13309848 | MS.gene058492:CDS |
| TTCAGGTCAAGAAGAATACT+AGG | 0.473719 | 4.1:+13309679 | MS.gene058492:CDS |
| GGTTTGGTGAAAACTGCGTT+TGG | 0.476936 | 4.1:-13309264 | None:intergenic |
| CCATCTCAGAATTGAAAAGA+AGG | 0.510329 | 4.1:+13308860 | MS.gene058492:CDS |
| AAAGCAAAACAACGATGACT+TGG | 0.515541 | 4.1:-13309285 | None:intergenic |
| AGAAATGGAATCAGCAAACT+GGG | 0.539408 | 4.1:+13309212 | MS.gene058492:CDS |
| TTTGGTGAATATTGTGAAGA+GGG | 0.541008 | 4.1:-13309374 | None:intergenic |
| CAAAATCAGCAATGAACAAG+AGG | 0.553083 | 4.1:+13308503 | MS.gene058492:CDS |
| TGGTGAAAACTGCGTTTGGA+TGG | 0.556904 | 4.1:-13309260 | None:intergenic |
| GTGGTGCCTGGTTTCAACAT+TGG | 0.564074 | 4.1:+13309807 | MS.gene058492:CDS |
| TAATTCCGGCGAGTTTCGGG+TGG | 0.572386 | 4.1:+13309776 | MS.gene058492:CDS |
| AGCAGCTGATCAAGCAGTGG+TGG | 0.584305 | 4.1:+13308825 | MS.gene058492:CDS |
| GAAAACCACCCGAAACTCGC+CGG | 0.587240 | 4.1:-13309781 | None:intergenic |
| ATTTGATGGTGATCTGTGAA+AGG | 0.594388 | 4.1:-13308646 | None:intergenic |
| AGTTTCAACTTCATGTTCAG+GGG | 0.594405 | 4.1:+13309740 | MS.gene058492:CDS |
| CAACTCACATCTCAAAGTGA+AGG | 0.600233 | 4.1:+13308612 | MS.gene058492:CDS |
| TGTTTGGAAATTTGAACCAG+AGG | 0.621071 | 4.1:+13309535 | MS.gene058492:CDS |
| ATTTGCAAAGACATTCCAGA+AGG | 0.624146 | 4.1:+13308534 | MS.gene058492:CDS |
| TTATGGAGGCTCTAATAGCA+AGG | 0.629498 | 4.1:+13308719 | MS.gene058492:CDS |
| AGGAAGTTAGTGAATTCTGG+AGG | 0.632480 | 4.1:+13309555 | MS.gene058492:CDS |
| GCTAGCATTATAGTAACTTG+AGG | 0.633614 | 4.1:-13308910 | None:intergenic |
| GTCTTACCAATGTTGAAACC+AGG | 0.633731 | 4.1:-13309813 | None:intergenic |
| TAGAAACAAAGCAGTTATGG+AGG | 0.646650 | 4.1:+13308705 | MS.gene058492:CDS |
| GGAAGTTAGTGAATTCTGGA+GGG | 0.667121 | 4.1:+13309556 | MS.gene058492:CDS |
| GAAGAATACTAGGTTCTCTG+AGG | 0.672182 | 4.1:+13309689 | MS.gene058492:CDS |
| ACAAGCAGCTGATCAAGCAG+TGG | 0.679340 | 4.1:+13308822 | MS.gene058492:CDS |
| TAAGCACCAACAGAAGAACA+AGG | 0.710444 | 4.1:+13308681 | MS.gene058492:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAAATTGCTTCAAATAA+TGG | + | chr4.1:13308577-13308596 | MS.gene058492:CDS | 20.0% |
| !!! | ATATTTGGATTTTAGAAACT+TGG | - | chr4.1:13309481-13309500 | None:intergenic | 20.0% |
| !!! | ATTTGTCAAAATAGTGATTT+TGG | - | chr4.1:13309395-13309414 | None:intergenic | 20.0% |
| !!! | TACTATTAGTTTTCATTTGA+TGG | - | chr4.1:13308663-13308682 | None:intergenic | 20.0% |
| ! | GAAACTTATGATTGAAGAAA+TGG | + | chr4.1:13309197-13309216 | MS.gene058492:CDS | 25.0% |
| ! | TGTATTGAATCATTGTTGTA+AGG | - | chr4.1:13308805-13308824 | None:intergenic | 25.0% |
| ! | TTCTTATTGATCTCAAAGTA+TGG | - | chr4.1:13309170-13309189 | None:intergenic | 25.0% |
| !! | TCATTTGAAACAGTGAAATT+TGG | - | chr4.1:13309354-13309373 | None:intergenic | 25.0% |
| !!! | ACTATTTTGACAAATTCAAG+AGG | + | chr4.1:13309400-13309419 | MS.gene058492:CDS | 25.0% |
| !!! | GATTCTATTGATTCTAATTC+CGG | + | chr4.1:13309762-13309781 | MS.gene058492:CDS | 25.0% |
| !!! | TTGTTTTCTAAGTACTGTTT+TGG | - | chr4.1:13309441-13309460 | None:intergenic | 25.0% |
| ATCATATGCAGAACTTCAAA+TGG | + | chr4.1:13308771-13308790 | MS.gene058492:CDS | 30.0% | |
| GATTCAACATTGAAAGTTCA+AGG | - | chr4.1:13309122-13309141 | None:intergenic | 30.0% | |
| TTTGGTGAATATTGTGAAGA+GGG | - | chr4.1:13309377-13309396 | None:intergenic | 30.0% | |
| ! | AAAATGGAGTGTTCTTTGTT+TGG | + | chr4.1:13309519-13309538 | MS.gene058492:CDS | 30.0% |
| ! | AGAAGATGTTAGCATTTTTC+AGG | + | chr4.1:13309662-13309681 | MS.gene058492:CDS | 30.0% |
| ! | ATGGACAACTTGATGATATT+TGG | - | chr4.1:13309496-13309515 | None:intergenic | 30.0% |
| ! | TTCTGCATATGATGCTTTTA+TGG | - | chr4.1:13308764-13308783 | None:intergenic | 30.0% |
| ! | TTTTGGTGAATATTGTGAAG+AGG | - | chr4.1:13309378-13309397 | None:intergenic | 30.0% |
| !!! | ACTCATCAAGTTTTTTTCTG+AGG | - | chr4.1:13309032-13309051 | None:intergenic | 30.0% |
| !!! | CTCATCAAGTTTTTTTCTGA+GGG | - | chr4.1:13309031-13309050 | None:intergenic | 30.0% |
| AAAGCAAAACAACGATGACT+TGG | - | chr4.1:13309288-13309307 | None:intergenic | 35.0% | |
| AACTTGGCAAAAGATGAGTT+TGG | - | chr4.1:13309465-13309484 | None:intergenic | 35.0% | |
| AAGAAATGGAATCAGCAAAC+TGG | + | chr4.1:13309211-13309230 | MS.gene058492:CDS | 35.0% | |
| AGAAATGGAATCAGCAAACT+GGG | + | chr4.1:13309212-13309231 | MS.gene058492:CDS | 35.0% | |
| AGTTTCAACTTCATGTTCAG+GGG | + | chr4.1:13309740-13309759 | MS.gene058492:CDS | 35.0% | |
| ATTTGCAAAGACATTCCAGA+AGG | + | chr4.1:13308534-13308553 | MS.gene058492:CDS | 35.0% | |
| CAAAATCAGCAATGAACAAG+AGG | + | chr4.1:13308503-13308522 | MS.gene058492:CDS | 35.0% | |
| CAGTTTCAACTTCATGTTCA+GGG | + | chr4.1:13309739-13309758 | MS.gene058492:CDS | 35.0% | |
| CCATCTCAGAATTGAAAAGA+AGG | + | chr4.1:13308860-13308879 | MS.gene058492:CDS | 35.0% | |
| GCTAAGAAAAAAGCTGAATC+TGG | - | chr4.1:13309585-13309604 | None:intergenic | 35.0% | |
| GCTAGCATTATAGTAACTTG+AGG | - | chr4.1:13308913-13308932 | None:intergenic | 35.0% | |
| GGTTAGAAACAAAGCAGTTA+TGG | + | chr4.1:13308702-13308721 | MS.gene058492:CDS | 35.0% | |
| TAGAAACAAAGCAGTTATGG+AGG | + | chr4.1:13308705-13308724 | MS.gene058492:CDS | 35.0% | |
| TCAGTTTCAACTTCATGTTC+AGG | + | chr4.1:13309738-13309757 | MS.gene058492:CDS | 35.0% | |
| TGTTTGGAAATTTGAACCAG+AGG | + | chr4.1:13309535-13309554 | MS.gene058492:CDS | 35.0% | |
| TTCAGGTCAAGAAGAATACT+AGG | + | chr4.1:13309679-13309698 | MS.gene058492:CDS | 35.0% | |
| TTCTAACCTTGTTCTTCTGT+TGG | - | chr4.1:13308690-13308709 | None:intergenic | 35.0% | |
| ! | TCAAGTTGTCCATGCTAAAA+TGG | + | chr4.1:13309503-13309522 | MS.gene058492:CDS | 35.0% |
| !! | AAAACAACGATGACTTGGTT+TGG | - | chr4.1:13309283-13309302 | None:intergenic | 35.0% |
| !! | AAGAACACTCCATTTTAGCA+TGG | - | chr4.1:13309515-13309534 | None:intergenic | 35.0% |
| !! | ATTTGATGGTGATCTGTGAA+AGG | - | chr4.1:13308649-13308668 | None:intergenic | 35.0% |
| !! | CCTTCTTTTCAATTCTGAGA+TGG | - | chr4.1:13308863-13308882 | None:intergenic | 35.0% |
| !! | CTTCTTGATGGTGATTTCAT+AGG | - | chr4.1:13308968-13308987 | None:intergenic | 35.0% |
| !! | TTTCTTAGCTTTTGCTGAGA+TGG | + | chr4.1:13309596-13309615 | MS.gene058492:CDS | 35.0% |
| !!! | CTCTTGTTCATTGCTGATTT+TGG | - | chr4.1:13308505-13308524 | None:intergenic | 35.0% |
| !!! | TTTTGAGACTAATCACCTTC+TGG | - | chr4.1:13308552-13308571 | None:intergenic | 35.0% |
| AGGAAGTTAGTGAATTCTGG+AGG | + | chr4.1:13309555-13309574 | MS.gene058492:CDS | 40.0% | |
| ATCACCATCAAGAAGCTTCA+AGG | + | chr4.1:13308973-13308992 | MS.gene058492:CDS | 40.0% | |
| ATTCTAATTCCGGCGAGTTT+CGG | + | chr4.1:13309772-13309791 | MS.gene058492:CDS | 40.0% | |
| CAACTCACATCTCAAAGTGA+AGG | + | chr4.1:13308612-13308631 | MS.gene058492:CDS | 40.0% | |
| CAGAATTCACTAACTTCCTC+TGG | - | chr4.1:13309554-13309573 | None:intergenic | 40.0% | |
| CAGAGGAAGTTAGTGAATTC+TGG | + | chr4.1:13309552-13309571 | MS.gene058492:CDS | 40.0% | |
| GAAGAATACTAGGTTCTCTG+AGG | + | chr4.1:13309689-13309708 | MS.gene058492:CDS | 40.0% | |
| GAGTCAGGTTTATCTGTCTT+TGG | + | chr4.1:13309845-13309864 | MS.gene058492:CDS | 40.0% | |
| GGAAGTTAGTGAATTCTGGA+GGG | + | chr4.1:13309556-13309575 | MS.gene058492:CDS | 40.0% | |
| GTCTTACCAATGTTGAAACC+AGG | - | chr4.1:13309816-13309835 | None:intergenic | 40.0% | |
| TAAGACAGTGATACAGAGTC+AGG | + | chr4.1:13309830-13309849 | MS.gene058492:CDS | 40.0% | |
| TAAGCACCAACAGAAGAACA+AGG | + | chr4.1:13308681-13308700 | MS.gene058492:CDS | 40.0% | |
| TCAGGTTTATCTGTCTTTGG+TGG | + | chr4.1:13309848-13309867 | MS.gene058492:CDS | 40.0% | |
| TGTTTGGACACTGCATTACT+TGG | + | chr4.1:13309626-13309645 | MS.gene058492:CDS | 40.0% | |
| TTCTCCTTGAAGCTTCTTGA+TGG | - | chr4.1:13308980-13308999 | None:intergenic | 40.0% | |
| ! | CTAATAGCAAGGCTTTTTGC+TGG | + | chr4.1:13308730-13308749 | MS.gene058492:CDS | 40.0% |
| ! | TTATGGAGGCTCTAATAGCA+AGG | + | chr4.1:13308719-13308738 | MS.gene058492:CDS | 40.0% |
| !! | TAGGTTCTCTGAGGTTTACA+TGG | + | chr4.1:13309698-13309717 | MS.gene058492:CDS | 40.0% |
| TGGTGAAAACTGCGTTTGGA+TGG | - | chr4.1:13309263-13309282 | None:intergenic | 45.0% | |
| TTCTAATTCCGGCGAGTTTC+GGG | + | chr4.1:13309773-13309792 | MS.gene058492:CDS | 45.0% | |
| !! | GGTTTGGTGAAAACTGCGTT+TGG | - | chr4.1:13309267-13309286 | None:intergenic | 45.0% |
| ACAAGCAGCTGATCAAGCAG+TGG | + | chr4.1:13308822-13308841 | MS.gene058492:CDS | 50.0% | |
| CTGAGATGGCAAAGCGTGTT+TGG | + | chr4.1:13309610-13309629 | MS.gene058492:CDS | 50.0% | |
| GTGGTGCCTGGTTTCAACAT+TGG | + | chr4.1:13309807-13309826 | MS.gene058492:CDS | 50.0% | |
| !! | CGAGTTTCGGGTGGTTTTCA+CGG | + | chr4.1:13309785-13309804 | MS.gene058492:CDS | 50.0% |
| AGCAGCTGATCAAGCAGTGG+TGG | + | chr4.1:13308825-13308844 | MS.gene058492:CDS | 55.0% | |
| GAAAACCACCCGAAACTCGC+CGG | - | chr4.1:13309784-13309803 | None:intergenic | 55.0% | |
| TAATTCCGGCGAGTTTCGGG+TGG | + | chr4.1:13309776-13309795 | MS.gene058492:CDS | 55.0% | |
| !! | GTTTCGGGTGGTTTTCACGG+TGG | + | chr4.1:13309788-13309807 | MS.gene058492:CDS | 55.0% |
| ! | GTGGTTTTCACGGTGGTGCC+TGG | + | chr4.1:13309795-13309814 | MS.gene058492:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 13308487 | 13309890 | 13308487 | ID=MS.gene058492 |
| chr4.1 | mRNA | 13308487 | 13309890 | 13308487 | ID=MS.gene058492.t1;Parent=MS.gene058492 |
| chr4.1 | exon | 13308487 | 13309890 | 13308487 | ID=MS.gene058492.t1.exon1;Parent=MS.gene058492.t1 |
| chr4.1 | CDS | 13308487 | 13309890 | 13308487 | ID=cds.MS.gene058492.t1;Parent=MS.gene058492.t1 |
| Gene Sequence |
| Protein sequence |
