AlfalfaGEDB | MS.gene08865

M. sativa cultivar XinJiangDaYe / MS.gene08865

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08865.t1	RHN42498.1	95.3	127	6	0	1	127	82	208	7.30E-48	199.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08865.t1	G7LH97	96.0	126	5	0	2	127	1	126	5.3e-48	199.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050463	MS.gene08865	0.876756	1.08E-68	-1.69E-46
MS.gene050528	MS.gene08865	0.873514	1.39E-67	-1.69E-46
MS.gene051946	MS.gene08865	0.829277	5.63E-55	-1.69E-46
MS.gene053175	MS.gene08865	0.811103	8.28E-51	-1.69E-46
MS.gene054549	MS.gene08865	0.807988	3.86E-50	-1.69E-46
MS.gene057014	MS.gene08865	0.817895	2.60E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08865.t1	MTR_8g097040	94.574	129	7	0	2	130	1	129	1.02e-84	243
MS.gene08865.t1	MTR_3g084990	55.147	136	47	4	1	130	1	128	2.77e-42	136
MS.gene08865.t1	MTR_5g078400	54.054	74	27	2	17	90	5	71	1.23e-16	69.7
MS.gene08865.t1	MTR_7g028805	42.308	130	60	3	1	117	1	128	1.30e-16	71.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08865.t1	AT1G27290	51.825	137	57	4	1	130	1	135	8.83e-38	125
MS.gene08865.t1	AT1G27290	51.825	137	57	4	1	130	1	135	8.83e-38	125

Find 34 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGATCGGTTACTTTCGTGAT+TGG	0.305883	4.2:+13791186	MS.gene08865:CDS
ATGTTGATGCCGTGGGTTAT+TGG	0.333475	4.2:+13791213	MS.gene08865:CDS
CATAGATTATGATTTCTTAC+CGG	0.352525	4.2:-13791331	None:intergenic
GTGGGTTATTGGATTGGTTA+TGG	0.372418	4.2:+13791224	MS.gene08865:CDS
GGATTTGCGTCGTTTGTTCT+CGG	0.413462	4.2:+13791138	MS.gene08865:CDS
GATTTGCGTCGTTTGTTCTC+GGG	0.415303	4.2:+13791139	MS.gene08865:CDS
GTCGTTTGTTCTCGGGATTT+CGG	0.429794	4.2:+13791146	MS.gene08865:CDS
GATCGGTTACTTTCGTGATT+GGG	0.431361	4.2:+13791187	MS.gene08865:CDS
TTGAGACGTTCGTCGGCGGC+AGG	0.478548	4.2:+13791099	MS.gene08865:CDS
GGCTTTGATGCTGTGTGGAT+CGG	0.501281	4.2:+13791170	MS.gene08865:CDS
GATGCCGTGGGTTATTGGAT+TGG	0.512161	4.2:+13791218	MS.gene08865:CDS
CTCAACACCGACGGATTCAC+CGG	0.514030	4.2:-13791081	None:intergenic
GACGGATTCACCGGCACGAT+CGG	0.516430	4.2:-13791072	None:intergenic
GATCGTGCCGGTGAATCCGT+CGG	0.526152	4.2:+13791074	MS.gene08865:CDS
TGGCGAAGCAAAGCAAAGAA+TGG	0.536106	4.2:-13791295	None:intergenic
ATAGATTATGATTTCTTACC+GGG	0.542281	4.2:-13791330	None:intergenic
GTCGGTGTTGAGACGTTCGT+CGG	0.552778	4.2:+13791092	MS.gene08865:CDS
CGGCACGATCGGAGATCGAT+CGG	0.564859	4.2:-13791061	None:intergenic
CGATCGATCTCCGATCGTGC+CGG	0.583698	4.2:+13791062	MS.gene08865:CDS
TGGGTTTATGTTGATGCCGT+GGG	0.591957	4.2:+13791206	MS.gene08865:CDS
GATCGATCGGAATAAGATAA+CGG	0.597785	4.2:-13791048	None:intergenic
TTGGGTTTATGTTGATGCCG+TGG	0.602091	4.2:+13791205	MS.gene08865:CDS
GTTTGTTCTCGGGATTTCGG+TGG	0.621102	4.2:+13791149	MS.gene08865:CDS
GCAGGTCAGATAACTCCGGC+GGG	0.623444	4.2:+13791117	MS.gene08865:CDS
GACGAACGTCTCAACACCGA+CGG	0.630029	4.2:-13791090	None:intergenic
GGTGTTGAGACGTTCGTCGG+CGG	0.630502	4.2:+13791095	MS.gene08865:CDS
TCGCCACGTAAAGATTCTCC+CGG	0.632756	4.2:+13791312	MS.gene08865:CDS
TCGGTGGCTTTGATGCTGTG+TGG	0.645493	4.2:+13791165	MS.gene08865:CDS
GGCAGGTCAGATAACTCCGG+CGG	0.647696	4.2:+13791116	MS.gene08865:CDS
GGCGGCAGGTCAGATAACTC+CGG	0.648034	4.2:+13791113	MS.gene08865:CDS
TCGGAATAAGATAACGGCGT+CGG	0.648078	4.2:-13791042	None:intergenic
ACAAACGACGCAAATCCCGC+CGG	0.670719	4.2:-13791132	None:intergenic
ATAACCAATCCAATAACCCA+CGG	0.672738	4.2:-13791222	None:intergenic
TTACCGGGAGAATCTTTACG+TGG	0.685734	4.2:-13791315	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GTTTCTTTTCTTTCTGTTTT+GGG	+	chr4.2:13791267-13791286	MS.gene08865:CDS	25.0%
!!	TGTTTCTTTTCTTTCTGTTT+TGG	+	chr4.2:13791266-13791285	MS.gene08865:CDS	25.0%
	ATAACCAATCCAATAACCCA+CGG	-	chr4.2:13791225-13791244	None:intergenic	35.0%
	GATCGATCGGAATAAGATAA+CGG	-	chr4.2:13791051-13791070	None:intergenic	35.0%
!	GTTATGGTGTTTTATGTTGC+TGG	+	chr4.2:13791240-13791259	MS.gene08865:CDS	35.0%
!	GATCGGTTACTTTCGTGATT+GGG	+	chr4.2:13791187-13791206	MS.gene08865:CDS	40.0%
!!	GTGGGTTATTGGATTGGTTA+TGG	+	chr4.2:13791224-13791243	MS.gene08865:CDS	40.0%
	GATTTGCGTCGTTTGTTCTC+GGG	+	chr4.2:13791139-13791158	MS.gene08865:CDS	45.0%
	GGATTTGCGTCGTTTGTTCT+CGG	+	chr4.2:13791138-13791157	MS.gene08865:CDS	45.0%
	GTCGTTTGTTCTCGGGATTT+CGG	+	chr4.2:13791146-13791165	MS.gene08865:CDS	45.0%
	TGGCGAAGCAAAGCAAAGAA+TGG	-	chr4.2:13791298-13791317	None:intergenic	45.0%
	TGGGTTTATGTTGATGCCGT+GGG	+	chr4.2:13791206-13791225	MS.gene08865:CDS	45.0%
	TTACCGGGAGAATCTTTACG+TGG	-	chr4.2:13791318-13791337	None:intergenic	45.0%
	TTGGGTTTATGTTGATGCCG+TGG	+	chr4.2:13791205-13791224	MS.gene08865:CDS	45.0%
!	ATGTTGATGCCGTGGGTTAT+TGG	+	chr4.2:13791213-13791232	MS.gene08865:CDS	45.0%
!	GGATCGGTTACTTTCGTGAT+TGG	+	chr4.2:13791186-13791205	MS.gene08865:CDS	45.0%
!	TCGGAATAAGATAACGGCGT+CGG	-	chr4.2:13791045-13791064	None:intergenic	45.0%
	GTTTGTTCTCGGGATTTCGG+TGG	+	chr4.2:13791149-13791168	MS.gene08865:CDS	50.0%
	TCGCCACGTAAAGATTCTCC+CGG	+	chr4.2:13791312-13791331	MS.gene08865:CDS	50.0%
!	GATGCCGTGGGTTATTGGAT+TGG	+	chr4.2:13791218-13791237	MS.gene08865:CDS	50.0%
!	GGCTTTGATGCTGTGTGGAT+CGG	+	chr4.2:13791170-13791189	MS.gene08865:CDS	50.0%
	ACAAACGACGCAAATCCCGC+CGG	-	chr4.2:13791135-13791154	None:intergenic	55.0%
	GACGAACGTCTCAACACCGA+CGG	-	chr4.2:13791093-13791112	None:intergenic	55.0%
!	CTCAACACCGACGGATTCAC+CGG	-	chr4.2:13791084-13791103	None:intergenic	55.0%
!!	GTCGGTGTTGAGACGTTCGT+CGG	+	chr4.2:13791092-13791111	MS.gene08865:CDS	55.0%
!!	TCGGTGGCTTTGATGCTGTG+TGG	+	chr4.2:13791165-13791184	MS.gene08865:CDS	55.0%
	CGATCGATCTCCGATCGTGC+CGG	+	chr4.2:13791062-13791081	MS.gene08865:CDS	60.0%
	CGGCACGATCGGAGATCGAT+CGG	-	chr4.2:13791064-13791083	None:intergenic	60.0%
	GCAGGTCAGATAACTCCGGC+GGG	+	chr4.2:13791117-13791136	MS.gene08865:CDS	60.0%
	GGCAGGTCAGATAACTCCGG+CGG	+	chr4.2:13791116-13791135	MS.gene08865:CDS	60.0%
	GGCGGCAGGTCAGATAACTC+CGG	+	chr4.2:13791113-13791132	MS.gene08865:CDS	60.0%
!	GACGGATTCACCGGCACGAT+CGG	-	chr4.2:13791075-13791094	None:intergenic	60.0%
!	GATCGTGCCGGTGAATCCGT+CGG	+	chr4.2:13791074-13791093	MS.gene08865:CDS	60.0%
!	GGTGTTGAGACGTTCGTCGG+CGG	+	chr4.2:13791095-13791114	MS.gene08865:CDS	60.0%
	TTGAGACGTTCGTCGGCGGC+AGG	+	chr4.2:13791099-13791118	MS.gene08865:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	13790952	13791347	13790952	ID=MS.gene08865
chr4.2	mRNA	13790952	13791347	13790952	ID=MS.gene08865.t1;Parent=MS.gene08865
chr4.2	exon	13790952	13791347	13790952	ID=MS.gene08865.t1.exon1;Parent=MS.gene08865.t1
chr4.2	CDS	13790952	13791347	13790952	ID=cds.MS.gene08865.t1;Parent=MS.gene08865.t1

Gene Sequence

>MS.gene08865

ATGATGAGAAGAAATCAAGATCAAGAACAACAATCAAAGATGATTTGCGAATTATCATCTCTACTTTTCAATCTTCTTCTACAATCTTCACCGACGCCGTTATCTTATTCCGATCGATCTCCGATCGTGCCGGTGAATCCGTCGGTGTTGAGACGTTCGTCGGCGGCAGGTCAGATAACTCCGGCGGGATTTGCGTCGTTTGTTCTCGGGATTTCGGTGGCTTTGATGCTGTGTGGATCGGTTACTTTCGTGATTGGGTTTATGTTGATGCCGTGGGTTATTGGATTGGTTATGGTGTTTTATGTTGCTGGTGTTGTTTCTTTTCTTTCTGTTTTGGGTCGCTCCATTCTTTGCTTTGCTTCGCCACGTAAAGATTCTCCCGGTAAGAAATCATAA

Protein sequence

>MS.gene08865.t1

MMRRNQDQEQQSKMICELSSLLFNLLLQSSPTPLSYSDRSPIVPVNPSVLRRSSAAGQITPAGFASFVLGISVALMLCGSVTFVIGFMLMPWVIGLVMVFYVAGVVSFLSVLGRSILCFASPRKDSPGKKS