Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054549.t1 | XP_013454547.1 | 94 | 530 | 22 | 4 | 1 | 525 | 1 | 525 | 3.50E-266 | 927.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054549.t1 | A0A072UHH6 | 94.0 | 530 | 22 | 4 | 1 | 525 | 1 | 525 | 2.5e-266 | 927.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053175 | MS.gene054549 | 0.968097 | 3.40E-128 | -1.69E-46 |
| MS.gene054549 | MS.gene063742 | 0.809192 | 2.14E-50 | -1.69E-46 |
| MS.gene054549 | MS.gene063743 | 0.805273 | 1.45E-49 | -1.69E-46 |
| MS.gene054549 | MS.gene063746 | 0.81825 | 2.16E-52 | -1.69E-46 |
| MS.gene054549 | MS.gene063747 | 0.821924 | 3.12E-53 | -1.69E-46 |
| MS.gene054549 | MS.gene063748 | 0.809666 | 1.69E-50 | -1.69E-46 |
| MS.gene054549 | MS.gene08865 | 0.807988 | 3.86E-50 | -1.69E-46 |
| MS.gene054549 | MS.gene65429 | 0.964417 | 2.67E-123 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054549.t1 | MTR_4g007710 | 95.472 | 530 | 14 | 4 | 1 | 525 | 1 | 525 | 0.0 | 1025 |
| MS.gene054549.t1 | MTR_4g130310 | 49.682 | 471 | 200 | 10 | 60 | 519 | 51 | 495 | 7.16e-136 | 403 |
| MS.gene054549.t1 | MTR_4g130310 | 49.786 | 468 | 205 | 9 | 60 | 519 | 51 | 496 | 9.48e-136 | 402 |
| MS.gene054549.t1 | MTR_4g130340 | 48.879 | 223 | 98 | 3 | 58 | 267 | 13 | 232 | 6.58e-58 | 194 |
| MS.gene054549.t1 | MTR_1g084010 | 52.778 | 144 | 68 | 0 | 110 | 253 | 21 | 164 | 9.32e-45 | 160 |
| MS.gene054549.t1 | MTR_6g013040 | 38.049 | 205 | 109 | 5 | 60 | 262 | 1 | 189 | 5.13e-34 | 128 |
| MS.gene054549.t1 | MTR_6g013260 | 36.585 | 205 | 112 | 5 | 60 | 262 | 1 | 189 | 2.49e-30 | 118 |
| MS.gene054549.t1 | MTR_4g113870 | 71.111 | 45 | 11 | 1 | 186 | 228 | 15 | 59 | 7.34e-17 | 75.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054549.t1 | AT2G24100 | 44.906 | 530 | 216 | 13 | 1 | 519 | 1 | 465 | 6.42e-130 | 387 |
| MS.gene054549.t1 | AT4G30780 | 50.398 | 377 | 150 | 7 | 17 | 370 | 26 | 388 | 3.60e-112 | 345 |
| MS.gene054549.t1 | AT4G30780 | 35.429 | 175 | 92 | 7 | 354 | 525 | 433 | 589 | 1.61e-13 | 73.6 |
| MS.gene054549.t1 | AT3G05770 | 40.000 | 215 | 119 | 4 | 60 | 265 | 20 | 233 | 9.45e-37 | 140 |
| MS.gene054549.t1 | AT3G05770 | 40.000 | 215 | 119 | 4 | 60 | 265 | 20 | 233 | 1.33e-36 | 140 |
| MS.gene054549.t1 | AT1G54300 | 42.500 | 160 | 82 | 4 | 115 | 265 | 2 | 160 | 3.37e-29 | 117 |
Find 121 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCAGAGTTGAAATCGAATT+TGG | 0.132685 | 4.1:-87667454 | None:intergenic |
| ACCCTTCTGATCTTTCTTCT+TGG | 0.228703 | 4.1:-87667860 | None:intergenic |
| CAAGAGTAAAATGTTTGATT+TGG | 0.243000 | 4.1:+87674144 | MS.gene054549:CDS |
| CTGAGAAACTTATCAGATTC+AGG | 0.259989 | 4.1:-87673779 | None:intergenic |
| CAGAAGTGAACAAAATCTTA+TGG | 0.288359 | 4.1:+87669644 | MS.gene054549:CDS |
| CCCATGCAATCAAAGGTTTC+AGG | 0.301138 | 4.1:+87673747 | MS.gene054549:CDS |
| TGTTTGGAACAGTGTGATTC+AGG | 0.311820 | 4.1:+87667715 | MS.gene054549:intron |
| CAATGCCCTCAAGGTCTGTT+AGG | 0.323271 | 4.1:+87669405 | MS.gene054549:CDS |
| AAGAGTAAAATGTTTGATTT+GGG | 0.335503 | 4.1:+87674145 | MS.gene054549:CDS |
| TTCGATGTCGATGGATGATT+TGG | 0.335720 | 4.1:+87673838 | MS.gene054549:CDS |
| GGACTGCATGATGTGGAATC+AGG | 0.352036 | 4.1:+87669597 | MS.gene054549:CDS |
| AGCTAGGTGAGGTAATTTCT+TGG | 0.354560 | 4.1:-87669689 | None:intergenic |
| AGAAGAAGTGAAGGGAAAGC+TGG | 0.361169 | 4.1:+87669567 | MS.gene054549:CDS |
| CATTGAATTGAGTGATGGTT+TGG | 0.363177 | 4.1:+87669542 | MS.gene054549:CDS |
| ACAATAACTTCCAATGTTCC+TGG | 0.363801 | 4.1:-87668473 | None:intergenic |
| TCCGATTTCATACAATCCTT+TGG | 0.368995 | 4.1:+87667750 | MS.gene054549:CDS |
| TTGCGAAGCATAAGCTTGTA+TGG | 0.377568 | 4.1:+87668363 | MS.gene054549:CDS |
| GTCTAGATATGAAGGAGATT+TGG | 0.382647 | 4.1:+87668325 | MS.gene054549:CDS |
| TGCCACAAGGTATGCTTCCT+TGG | 0.393612 | 4.1:-87669055 | None:intergenic |
| TCCTGAAACCTTTGATTGCA+TGG | 0.393638 | 4.1:-87673748 | None:intergenic |
| TCCAAAGGATTGTATGAAAT+CGG | 0.396620 | 4.1:-87667751 | None:intergenic |
| TTTCTGAAGAAGGCAGTATA+AGG | 0.405521 | 4.1:-87674023 | None:intergenic |
| CAATCAACAACAGACTTTAC+TGG | 0.408931 | 4.1:+87669076 | MS.gene054549:CDS |
| GGAGAATCCAACACAAATTC+CGG | 0.411628 | 4.1:-87669486 | None:intergenic |
| CCACATCGGACACTGCATTT+CGG | 0.412120 | 4.1:+87673865 | MS.gene054549:CDS |
| GCAGTTCCAGTCTCAAAGTA+TGG | 0.416139 | 4.1:-87669507 | None:intergenic |
| CAAGATGATGATGATGCTTC+TGG | 0.418720 | 4.1:+87674175 | MS.gene054549:CDS |
| TCAGAGTTGAAATCGAATTT+GGG | 0.419622 | 4.1:-87667453 | None:intergenic |
| CTATCAGAATCCTCGGACAT+AGG | 0.423015 | 4.1:-87674295 | None:intergenic |
| CCTGAAACCTTTGATTGCAT+GGG | 0.423667 | 4.1:-87673747 | None:intergenic |
| TGTGATTCAGGTGATGATTC+TGG | 0.430308 | 4.1:+87667727 | MS.gene054549:CDS |
| CTTGTATGGGAAGTTCTTGA+TGG | 0.433138 | 4.1:+87668377 | MS.gene054549:CDS |
| AATGTTCCTGGAGCATCCTC+AGG | 0.433779 | 4.1:-87668461 | None:intergenic |
| CCAACACAAATTCCGGTTGT+TGG | 0.433853 | 4.1:-87669479 | None:intergenic |
| GGTAGCCAGCAGCAAGGAAA+TGG | 0.434094 | 4.1:+87674196 | MS.gene054549:CDS |
| AAGGTATGCTTCCTTGGTTG+TGG | 0.434236 | 4.1:-87669049 | None:intergenic |
| AGCTCTGTCATTAGTGAAGC+TGG | 0.450625 | 4.1:-87673909 | None:intergenic |
| AGAAGTGAACAAAATCTTAT+GGG | 0.452591 | 4.1:+87669645 | MS.gene054549:CDS |
| TTATTTATACCTGTGTATGC+TGG | 0.455133 | 4.1:-87669107 | None:intergenic |
| CAACTTTCTGAGCCAACAAC+CGG | 0.458040 | 4.1:+87669467 | MS.gene054549:CDS |
| CTGAAACCTTTGATTGCATG+GGG | 0.466395 | 4.1:-87673746 | None:intergenic |
| GATTCTCCATACTTTGAGAC+TGG | 0.468589 | 4.1:+87669501 | MS.gene054549:CDS |
| GAAGAAGTGAAGGGAAAGCT+GGG | 0.473769 | 4.1:+87669568 | MS.gene054549:CDS |
| CACTTTGTGCAATGCCCTCA+AGG | 0.479139 | 4.1:+87669396 | MS.gene054549:CDS |
| ACAGACCATTTCCTTGCTGC+TGG | 0.483280 | 4.1:-87674201 | None:intergenic |
| CACTAATGACAGAGCTGTGT+TGG | 0.483298 | 4.1:+87673916 | MS.gene054549:CDS |
| GAAGAAGAGTCCTTCACTGT+TGG | 0.484620 | 4.1:+87667801 | MS.gene054549:CDS |
| AATTCCATGGTCTGATATCA+TGG | 0.485625 | 4.1:+87668421 | MS.gene054549:CDS |
| CCAATCTTCAAAACAGTAGC+AGG | 0.487934 | 4.1:-87667940 | None:intergenic |
| TTTCAACTCTGATTCTCCTA+AGG | 0.489774 | 4.1:+87667464 | MS.gene054549:CDS |
| CCAACAACCGGAATTTGTGT+TGG | 0.491090 | 4.1:+87669479 | MS.gene054549:CDS |
| TTGAGTCAGATTAAGTTGCC+TGG | 0.498526 | 4.1:+87673806 | MS.gene054549:CDS |
| AGACGCAAACCAAGTGGACT+TGG | 0.503986 | 4.1:-87667778 | None:intergenic |
| TCTTCACCCTTCGATGTCGA+TGG | 0.504190 | 4.1:+87673829 | MS.gene054549:CDS |
| CAGTATAAGTCTAGATATGA+AGG | 0.517526 | 4.1:+87668317 | MS.gene054549:intron |
| ATTTGCTTCTGAATCTTCCA+AGG | 0.519721 | 4.1:+87674245 | MS.gene054549:CDS |
| CACTCAATTCAATGTGGTCA+TGG | 0.523533 | 4.1:-87669533 | None:intergenic |
| TGTGGCAGAGAAGCAATCCT+TGG | 0.523882 | 4.1:-87674262 | None:intergenic |
| GAAATTACCTCACCTAGCTC+AGG | 0.524612 | 4.1:+87669693 | MS.gene054549:CDS |
| TGAGGATGCTCCAGGAACAT+TGG | 0.526671 | 4.1:+87668463 | MS.gene054549:CDS |
| GTCTGATATCATGGCTCTCA+AGG | 0.526777 | 4.1:+87668430 | MS.gene054549:CDS |
| TAATGACAGAGCTGTGTTGG+AGG | 0.526990 | 4.1:+87673919 | MS.gene054549:CDS |
| CATGATGTGGAATCAGGGTC+TGG | 0.530089 | 4.1:+87669603 | MS.gene054549:CDS |
| AGAATAAGATTGAAATTCCA+TGG | 0.535046 | 4.1:+87668408 | MS.gene054549:CDS |
| TTGGATGAACCAAGTCCACT+TGG | 0.535980 | 4.1:+87667769 | MS.gene054549:CDS |
| TTCTGTTCATCATCAGAGAC+AGG | 0.536856 | 4.1:-87673980 | None:intergenic |
| GACATCGAAGGGTGAAGACC+AGG | 0.537780 | 4.1:-87673824 | None:intergenic |
| TGCTGCTGATAGCAAGCTCA+AGG | 0.539666 | 4.1:+87667906 | MS.gene054549:CDS |
| TTGGCTCAGAAAGTTGAGAC+GGG | 0.539829 | 4.1:-87669460 | None:intergenic |
| CTGTCTAGGAAAGAATCAGC+TGG | 0.542082 | 4.1:+87674220 | MS.gene054549:CDS |
| AACTACCCTGAGGATGCTCC+AGG | 0.542158 | 4.1:+87668455 | MS.gene054549:CDS |
| GCCTCCCTAACAGACCTTGA+GGG | 0.542641 | 4.1:-87669410 | None:intergenic |
| ACACTGCATTTCGGAACAGA+TGG | 0.543363 | 4.1:+87673874 | MS.gene054549:CDS |
| AATGCCCTCAAGGTCTGTTA+GGG | 0.546969 | 4.1:+87669406 | MS.gene054549:CDS |
| TGTATTTGTTGTAGCTGGCA+AGG | 0.547557 | 4.1:+87668999 | MS.gene054549:intron |
| TGCGAAGCATAAGCTTGTAT+GGG | 0.558386 | 4.1:+87668364 | MS.gene054549:CDS |
| TTCCAAGAAGAAAGATCAGA+AGG | 0.560722 | 4.1:+87667858 | MS.gene054549:CDS |
| GCCCTCAAGGTCTGTTAGGG+AGG | 0.562789 | 4.1:+87669409 | MS.gene054549:CDS |
| GGTTTGGATAGAAGAAGTGA+AGG | 0.566531 | 4.1:+87669558 | MS.gene054549:CDS |
| GAGAAGGGTCTTTCTGAAGA+AGG | 0.568373 | 4.1:-87674033 | None:intergenic |
| AACATGACTATCAGAATCCT+CGG | 0.577952 | 4.1:-87674302 | None:intergenic |
| CACTGCATTTCGGAACAGAT+GGG | 0.578013 | 4.1:+87673875 | MS.gene054549:CDS |
| TAGATATGAAGGAGATTTGG+TGG | 0.584423 | 4.1:+87668328 | MS.gene054549:CDS |
| TGCCTCCCTAACAGACCTTG+AGG | 0.584503 | 4.1:-87669411 | None:intergenic |
| AGCAAGGAAATGGTCTGTCT+AGG | 0.585074 | 4.1:+87674206 | MS.gene054549:CDS |
| AAATCATCCATCGACATCGA+AGG | 0.585590 | 4.1:-87673836 | None:intergenic |
| AGTGGACTTGGTTCATCCAA+AGG | 0.586408 | 4.1:-87667766 | None:intergenic |
| CCATAGGAATTACCTGAGCT+AGG | 0.591916 | 4.1:-87669705 | None:intergenic |
| TTCTTCAGACGCAAACCAAG+TGG | 0.595315 | 4.1:-87667784 | None:intergenic |
| ATCAAAGGTTTCAGGAGCAG+AGG | 0.596879 | 4.1:+87673755 | MS.gene054549:CDS |
| GACTGCATGATGTGGAATCA+GGG | 0.597635 | 4.1:+87669598 | MS.gene054549:CDS |
| GACATAGGAAACAGAAAATG+TGG | 0.600386 | 4.1:-87674280 | None:intergenic |
| TGAATCAAATCCAACAGTGA+AGG | 0.602378 | 4.1:-87667811 | None:intergenic |
| GTTGGCTCAGAAAGTTGAGA+CGG | 0.604139 | 4.1:-87669461 | None:intergenic |
| GAAGATAGAAAAGTTGATGA+GGG | 0.606665 | 4.1:+87674106 | MS.gene054549:CDS |
| TTTGAAGATTGGTACTTGGG+AGG | 0.609526 | 4.1:+87667951 | MS.gene054549:CDS |
| GACCACATTGAATTGAGTGA+TGG | 0.611625 | 4.1:+87669537 | MS.gene054549:CDS |
| TGAAGATAGAAAAGTTGATG+AGG | 0.612436 | 4.1:+87674105 | MS.gene054549:CDS |
| AATCATCCATCGACATCGAA+GGG | 0.616354 | 4.1:-87673835 | None:intergenic |
| GTCTGTTGTTGATTGCCACA+AGG | 0.622596 | 4.1:-87669068 | None:intergenic |
| CTTGAGGGCATTGCACAAAG+TGG | 0.635048 | 4.1:-87669395 | None:intergenic |
| TCAACAACAGACTTTACTGG+TGG | 0.640598 | 4.1:+87669079 | MS.gene054549:CDS |
| ATGTTCCTGGAGCATCCTCA+GGG | 0.646838 | 4.1:-87668460 | None:intergenic |
| GTTTGGATAGAAGAAGTGAA+GGG | 0.651890 | 4.1:+87669559 | MS.gene054549:CDS |
| TCCAAGAAGAAAGATCAGAA+GGG | 0.651999 | 4.1:+87667859 | MS.gene054549:CDS |
| GTGGACAAGCCAGCATACAC+AGG | 0.655527 | 4.1:+87669098 | MS.gene054549:CDS |
| TGTTCATCATCAGAGACAGG+TGG | 0.655949 | 4.1:-87673977 | None:intergenic |
| ATTTGTTGTAGCTGGCAAGG+AGG | 0.658694 | 4.1:+87669002 | MS.gene054549:intron |
| GGGAGATCAATCCACAACCA+AGG | 0.673080 | 4.1:+87669038 | MS.gene054549:CDS |
| GCTTCTGGTAGCCAGCAGCA+AGG | 0.673116 | 4.1:+87674190 | MS.gene054549:CDS |
| ATGAACAGAATGTCATGACA+AGG | 0.678824 | 4.1:+87673993 | MS.gene054549:CDS |
| GGAATTACCTGAGCTAGGTG+AGG | 0.680636 | 4.1:-87669700 | None:intergenic |
| TGGCTCAGAAAGTTGAGACG+GGG | 0.681167 | 4.1:-87669459 | None:intergenic |
| GATGATTTGGTTAACCACAT+CGG | 0.685792 | 4.1:+87673851 | MS.gene054549:CDS |
| AGAGCCATGATATCAGACCA+TGG | 0.692062 | 4.1:-87668425 | None:intergenic |
| TGAAACCTTTGATTGCATGG+GGG | 0.696180 | 4.1:-87673745 | None:intergenic |
| CCGAAATGCAGTGTCCGATG+TGG | 0.700782 | 4.1:-87673865 | None:intergenic |
| AACCATCACTCAATTCAATG+TGG | 0.718712 | 4.1:-87669539 | None:intergenic |
| TCTCAAGGCAAACTACCCTG+AGG | 0.745164 | 4.1:+87668445 | MS.gene054549:CDS |
| TGAACAGAATGTCATGACAA+GGG | 0.758366 | 4.1:+87673994 | MS.gene054549:CDS |
| AACCAAGGAAGCATACCTTG+TGG | 0.758682 | 4.1:+87669053 | MS.gene054549:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 87667351 | 87674330 | 87667351 | ID=MS.gene054549 |
| chr4.1 | mRNA | 87667351 | 87674330 | 87667351 | ID=MS.gene054549.t1;Parent=MS.gene054549 |
| chr4.1 | exon | 87667351 | 87667485 | 87667351 | ID=MS.gene054549.t1.exon1;Parent=MS.gene054549.t1 |
| chr4.1 | CDS | 87667351 | 87667485 | 87667351 | ID=cds.MS.gene054549.t1;Parent=MS.gene054549.t1 |
| chr4.1 | exon | 87667727 | 87667972 | 87667727 | ID=MS.gene054549.t1.exon2;Parent=MS.gene054549.t1 |
| chr4.1 | CDS | 87667727 | 87667972 | 87667727 | ID=cds.MS.gene054549.t1;Parent=MS.gene054549.t1 |
| chr4.1 | exon | 87668320 | 87668493 | 87668320 | ID=MS.gene054549.t1.exon3;Parent=MS.gene054549.t1 |
| chr4.1 | CDS | 87668320 | 87668493 | 87668320 | ID=cds.MS.gene054549.t1;Parent=MS.gene054549.t1 |
| chr4.1 | exon | 87669013 | 87669119 | 87669013 | ID=MS.gene054549.t1.exon4;Parent=MS.gene054549.t1 |
| chr4.1 | CDS | 87669013 | 87669119 | 87669013 | ID=cds.MS.gene054549.t1;Parent=MS.gene054549.t1 |
| chr4.1 | exon | 87669392 | 87669714 | 87669392 | ID=MS.gene054549.t1.exon5;Parent=MS.gene054549.t1 |
| chr4.1 | CDS | 87669392 | 87669714 | 87669392 | ID=cds.MS.gene054549.t1;Parent=MS.gene054549.t1 |
| chr4.1 | exon | 87673738 | 87674330 | 87673738 | ID=MS.gene054549.t1.exon6;Parent=MS.gene054549.t1 |
| chr4.1 | CDS | 87673738 | 87674330 | 87673738 | ID=cds.MS.gene054549.t1;Parent=MS.gene054549.t1 |
| Gene Sequence |
| Protein sequence |
