Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09728.t1 | XP_003611369.1 | 96.7 | 548 | 16 | 2 | 1 | 546 | 1 | 548 | 8.80E-297 | 1028.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09728.t1 | O23522 | 55.4 | 531 | 197 | 10 | 25 | 546 | 18 | 517 | 5.3e-157 | 555.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09728.t1 | G7JXH0 | 96.7 | 548 | 16 | 2 | 1 | 546 | 1 | 548 | 6.3e-297 | 1028.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050238 | MS.gene09728 | 0.81354 | 2.43E-51 | -1.69E-46 |
| MS.gene051823 | MS.gene09728 | 0.808556 | 2.92E-50 | -1.69E-46 |
| MS.gene052016 | MS.gene09728 | 0.823868 | 1.10E-53 | -1.69E-46 |
| MS.gene060522 | MS.gene09728 | 0.80712 | 5.90E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09728.t1 | MTR_5g013230 | 97.628 | 548 | 11 | 2 | 1 | 546 | 1 | 548 | 0.0 | 1110 |
| MS.gene09728.t1 | MTR_4g099510 | 65.766 | 555 | 137 | 9 | 6 | 544 | 4 | 521 | 0.0 | 715 |
| MS.gene09728.t1 | MTR_7g081620 | 47.165 | 388 | 177 | 8 | 129 | 492 | 65 | 448 | 1.57e-120 | 362 |
| MS.gene09728.t1 | MTR_7g081645 | 45.553 | 371 | 167 | 9 | 133 | 492 | 75 | 421 | 8.94e-110 | 334 |
| MS.gene09728.t1 | MTR_4g077070 | 38.281 | 384 | 201 | 7 | 128 | 493 | 95 | 460 | 3.97e-84 | 271 |
| MS.gene09728.t1 | MTR_7g096120 | 39.737 | 380 | 194 | 11 | 133 | 493 | 74 | 437 | 1.12e-81 | 264 |
| MS.gene09728.t1 | MTR_7g096140 | 39.211 | 380 | 196 | 11 | 133 | 493 | 74 | 437 | 5.42e-81 | 261 |
| MS.gene09728.t1 | MTR_2g015690 | 35.156 | 384 | 192 | 9 | 133 | 493 | 20 | 369 | 1.28e-63 | 214 |
| MS.gene09728.t1 | MTR_7g006400 | 32.683 | 410 | 215 | 13 | 109 | 500 | 80 | 446 | 4.17e-57 | 199 |
| MS.gene09728.t1 | MTR_4g087810 | 33.075 | 387 | 195 | 11 | 133 | 495 | 13 | 359 | 6.13e-52 | 182 |
| MS.gene09728.t1 | MTR_4g087830 | 33.333 | 387 | 194 | 12 | 133 | 495 | 4 | 350 | 2.82e-51 | 181 |
| MS.gene09728.t1 | MTR_4g080100 | 29.353 | 402 | 214 | 9 | 129 | 493 | 24 | 392 | 1.01e-48 | 175 |
| MS.gene09728.t1 | MTR_1995s0010 | 40.928 | 237 | 114 | 7 | 251 | 475 | 1 | 223 | 5.20e-45 | 158 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09728.t1 | AT4G16820 | 55.367 | 531 | 197 | 10 | 25 | 546 | 18 | 517 | 0.0 | 562 |
| MS.gene09728.t1 | AT2G44810 | 50.136 | 369 | 170 | 7 | 133 | 494 | 86 | 447 | 5.25e-123 | 369 |
| MS.gene09728.t1 | AT2G44810 | 50.413 | 363 | 166 | 7 | 139 | 494 | 2 | 357 | 1.95e-120 | 359 |
| MS.gene09728.t1 | AT1G30370 | 38.015 | 413 | 219 | 10 | 124 | 517 | 105 | 499 | 6.99e-86 | 276 |
| MS.gene09728.t1 | AT1G51440 | 41.463 | 369 | 172 | 10 | 137 | 481 | 91 | 439 | 3.66e-83 | 268 |
| MS.gene09728.t1 | AT1G06800 | 39.633 | 381 | 187 | 10 | 137 | 493 | 89 | 450 | 5.88e-79 | 257 |
| MS.gene09728.t1 | AT2G30550 | 39.227 | 362 | 186 | 7 | 137 | 479 | 105 | 451 | 9.75e-79 | 255 |
| MS.gene09728.t1 | AT1G06800 | 40.385 | 364 | 174 | 10 | 137 | 476 | 89 | 433 | 1.11e-78 | 254 |
| MS.gene09728.t1 | AT2G30550 | 38.830 | 376 | 196 | 8 | 137 | 493 | 105 | 465 | 6.99e-78 | 255 |
| MS.gene09728.t1 | AT2G31690 | 35.989 | 364 | 182 | 11 | 133 | 475 | 88 | 421 | 2.66e-65 | 220 |
| MS.gene09728.t1 | AT1G05800 | 35.833 | 360 | 185 | 9 | 133 | 475 | 80 | 410 | 1.00e-63 | 216 |
| MS.gene09728.t1 | AT4G18550 | 33.012 | 415 | 210 | 11 | 133 | 515 | 19 | 397 | 1.85e-60 | 206 |
| MS.gene09728.t1 | AT4G18550 | 33.012 | 415 | 210 | 11 | 133 | 515 | 36 | 414 | 2.72e-60 | 206 |
| MS.gene09728.t1 | AT1G06250 | 31.959 | 388 | 200 | 10 | 133 | 493 | 5 | 355 | 6.52e-54 | 188 |
| MS.gene09728.t1 | AT2G42690 | 32.468 | 385 | 204 | 8 | 137 | 493 | 8 | 364 | 3.55e-51 | 181 |
| MS.gene09728.t1 | AT4G18550 | 36.806 | 288 | 151 | 8 | 133 | 397 | 36 | 315 | 4.56e-47 | 167 |
| MS.gene09728.t1 | AT1G06250 | 30.797 | 276 | 140 | 7 | 234 | 493 | 1 | 241 | 5.10e-32 | 125 |
Find 139 sgRNAs with CRISPR-Local
Find 154 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCAATTGGATTACCTAAA+TGG | 0.127660 | 5.3:+6144164 | MS.gene09728:CDS |
| CCTCGGGTAAATCGACTAAT+TGG | 0.206931 | 5.3:-6144357 | None:intergenic |
| GAAGACGCTGTAAGCGTTTC+AGG | 0.238955 | 5.3:-6143877 | None:intergenic |
| GTTACTTTATAAGATCTTTC+CGG | 0.250527 | 5.3:-6144121 | None:intergenic |
| TAACCTAGATGAGTTGTTAT+TGG | 0.251262 | 5.3:-6143666 | None:intergenic |
| CTCTTAGCATTAGCTCTAAA+TGG | 0.258178 | 5.3:-6144985 | None:intergenic |
| CGCCTTTGTAAAGTTCAATA+AGG | 0.276950 | 5.3:-6144510 | None:intergenic |
| AAAAGATGAAGGTTGTTTAA+TGG | 0.278707 | 5.3:-6143630 | None:intergenic |
| TTTCTCCCGATGATATTTCT+TGG | 0.282496 | 5.3:-6143923 | None:intergenic |
| AGAGGCTTATTTACATTTAG+TGG | 0.289753 | 5.3:+6144939 | MS.gene09728:CDS |
| ATGATGATTCCAAAGGCTTT+TGG | 0.306541 | 5.3:-6143721 | None:intergenic |
| GCCACCTCATCAACCCATTT+AGG | 0.326253 | 5.3:-6144178 | None:intergenic |
| GTTGTATTGGTTGTTGAAAC+TGG | 0.332759 | 5.3:-6143751 | None:intergenic |
| ACTCAACTTTGGGCTTTGAT+TGG | 0.339758 | 5.3:-6144408 | None:intergenic |
| ACAGCCACGGGTGGCACGTT+TGG | 0.344135 | 5.3:-6144616 | None:intergenic |
| TAATTTCCTCGGCCACCAAT+AGG | 0.366166 | 5.3:-6144582 | None:intergenic |
| CGGCGAGAGGTTGTTAGATA+TGG | 0.368122 | 5.3:+6144010 | MS.gene09728:CDS |
| CATAAATCCACACTCAACTT+TGG | 0.371336 | 5.3:-6144419 | None:intergenic |
| CTGTAAGCGTTTCAGGTGGC+GGG | 0.378709 | 5.3:-6143870 | None:intergenic |
| TCTTCAGCTGCTTTAATCTC+TGG | 0.381476 | 5.3:-6143839 | None:intergenic |
| TGGCGCGACACTAGCCCTAT+TGG | 0.385796 | 5.3:+6144567 | MS.gene09728:CDS |
| AACTTCCACTTGTCTTGAAT+GGG | 0.386150 | 5.3:+6144318 | MS.gene09728:CDS |
| GTTGGAAGAGAACACTCCTT+TGG | 0.394244 | 5.3:+6144825 | MS.gene09728:CDS |
| CGTGTTGGTAACAGAGCCTT+TGG | 0.394565 | 5.3:+6144658 | MS.gene09728:CDS |
| TAAAAGAGAGATTGCAAGAA+TGG | 0.395214 | 5.3:+6144264 | MS.gene09728:CDS |
| ATAAATCCACACTCAACTTT+GGG | 0.396883 | 5.3:-6144418 | None:intergenic |
| TCTTGATAGCCAAAAGCCTT+TGG | 0.405866 | 5.3:+6143712 | MS.gene09728:CDS |
| GGATGACCCAGCGTTCTAGC+TGG | 0.407435 | 5.3:+6144215 | MS.gene09728:CDS |
| ATCAATTGGATTACCTAAAT+GGG | 0.408997 | 5.3:+6144165 | MS.gene09728:CDS |
| GGTTGTTAGATATGGTGATT+TGG | 0.409569 | 5.3:+6144018 | MS.gene09728:CDS |
| GAACTTCCACTTGTCTTGAA+TGG | 0.412888 | 5.3:+6144317 | MS.gene09728:CDS |
| GACTTAACCTAGCAAAACTA+TGG | 0.417487 | 5.3:+6143816 | MS.gene09728:CDS |
| CTGTATGCCACATCATCAAT+TGG | 0.418121 | 5.3:+6144151 | MS.gene09728:CDS |
| TCATCAAGAGGTGTCGTTGC+CGG | 0.419070 | 5.3:+6144102 | MS.gene09728:CDS |
| AATTTCCTCGGCCACCAATA+GGG | 0.421437 | 5.3:-6144581 | None:intergenic |
| ATGAGCTTGTACAAGACAAA+AGG | 0.423174 | 5.3:+6144439 | MS.gene09728:CDS |
| TTGGAAGAGAACACTCCTTT+GGG | 0.425192 | 5.3:+6144826 | MS.gene09728:CDS |
| GAATTAAGCATAACCGTTAC+CGG | 0.425326 | 5.3:+6144535 | MS.gene09728:CDS |
| AAGAATTCCAAGGTTGTTAG+TGG | 0.426321 | 5.3:+6144790 | MS.gene09728:CDS |
| ACATTTAGTGGATGGATACA+TGG | 0.441165 | 5.3:+6144951 | MS.gene09728:CDS |
| AAACCAATAACAACTCATCT+AGG | 0.443716 | 5.3:+6143663 | MS.gene09728:CDS |
| AATATCATGGCAGCAACGAC+TGG | 0.446528 | 5.3:+6143954 | MS.gene09728:CDS |
| GATGAGGTGGCACCAGATCT+TGG | 0.447657 | 5.3:+6144190 | MS.gene09728:CDS |
| CCCGCACGTGCTAATTTCCT+CGG | 0.448005 | 5.3:-6144593 | None:intergenic |
| TAGACAGGGATCCATGTCTA+TGG | 0.450095 | 5.3:+6145125 | MS.gene09728:CDS |
| GTTGTTTAATGGAAGAAGAA+TGG | 0.450283 | 5.3:-6143619 | None:intergenic |
| CACTTCTTCAACAACGGACT+CGG | 0.454486 | 5.3:-6144482 | None:intergenic |
| GTGAAGGTTGTGAGCAGGTA+AGG | 0.457213 | 5.3:-6143561 | None:intergenic |
| GTAAGCGTTTCAGGTGGCGG+GGG | 0.458362 | 5.3:-6143868 | None:intergenic |
| CTTGTCTAATCTTAGTAGAC+AGG | 0.459042 | 5.3:+6145110 | MS.gene09728:CDS |
| CTAGTGTCGCGCCAAGGCTA+TGG | 0.467757 | 5.3:-6144558 | None:intergenic |
| GCTGTAAGCGTTTCAGGTGG+CGG | 0.475077 | 5.3:-6143871 | None:intergenic |
| GTCTGAGTCTGAGCGTCCTC+GGG | 0.477426 | 5.3:-6144373 | None:intergenic |
| TCGCCGGAGGTTGTCATCCA+AGG | 0.480124 | 5.3:-6143993 | None:intergenic |
| GAAGGGGATGTTAGATCCCT+TGG | 0.480349 | 5.3:+6143976 | MS.gene09728:CDS |
| TGGGTCATCCAACCAAGATC+TGG | 0.480411 | 5.3:-6144202 | None:intergenic |
| TGAAGAGAAGCAAAAGATGA+AGG | 0.480894 | 5.3:-6143641 | None:intergenic |
| GCTTCAGTTGTTGACATGGC+AGG | 0.485069 | 5.3:-6144073 | None:intergenic |
| TGGAGAATACTCCGCCGTCT+TGG | 0.486287 | 5.3:-6143903 | None:intergenic |
| TATTGGTTGTTGAAACTGGT+TGG | 0.487668 | 5.3:-6143747 | None:intergenic |
| AGTCTGAGTCTGAGCGTCCT+CGG | 0.490474 | 5.3:-6144374 | None:intergenic |
| AAAAGAGAGATTGCAAGAAT+GGG | 0.490721 | 5.3:+6144265 | MS.gene09728:CDS |
| ACACACAAGACGTGATTACA+AGG | 0.492272 | 5.3:+6144725 | MS.gene09728:CDS |
| CAACGACACCTCTTGATGAT+GGG | 0.496077 | 5.3:-6144098 | None:intergenic |
| CTCGGGTAAATCGACTAATT+GGG | 0.499666 | 5.3:-6144356 | None:intergenic |
| AACAACTCCACTAACAACCT+TGG | 0.499945 | 5.3:-6144797 | None:intergenic |
| GCAACGACACCTCTTGATGA+TGG | 0.502309 | 5.3:-6144099 | None:intergenic |
| TGAGCTTGTACAAGACAAAA+GGG | 0.503559 | 5.3:+6144440 | MS.gene09728:CDS |
| CCTTTGGGATATTCACATGT+TGG | 0.504000 | 5.3:+6144841 | MS.gene09728:CDS |
| GAAATATCATCGGGAGAAAG+TGG | 0.505201 | 5.3:+6143927 | MS.gene09728:CDS |
| CTTCAACAACGGACTCGGAT+AGG | 0.508893 | 5.3:-6144477 | None:intergenic |
| TCAGCATCGGGCTTCAAGTA+AGG | 0.510052 | 5.3:-6144895 | None:intergenic |
| CAACAAGCTATGTCAGCATC+GGG | 0.515189 | 5.3:-6144907 | None:intergenic |
| GCTTATTTACATTTAGTGGA+TGG | 0.516513 | 5.3:+6144943 | MS.gene09728:CDS |
| GATGACCCAGCGTTCTAGCT+GGG | 0.518537 | 5.3:+6144216 | MS.gene09728:CDS |
| ACCCAGCGTTCTAGCTGGGT+TGG | 0.519887 | 5.3:+6144220 | MS.gene09728:CDS |
| GAAGAATGGCATCTGAAACT+AGG | 0.523536 | 5.3:-6143605 | None:intergenic |
| TTGTATACTAGTAAGGCTAA+AGG | 0.526986 | 5.3:+6145054 | MS.gene09728:CDS |
| ATCCCTTGGATGACAACCTC+CGG | 0.528647 | 5.3:+6143990 | MS.gene09728:CDS |
| ATCCAACCCAGCTAGAACGC+TGG | 0.529696 | 5.3:-6144222 | None:intergenic |
| GACGTGATTACAAGGGTGCC+AGG | 0.535150 | 5.3:+6144733 | MS.gene09728:CDS |
| CGCGACACTAGCCCTATTGG+TGG | 0.536052 | 5.3:+6144570 | MS.gene09728:CDS |
| TAGTGGAGTTGTTGATATGT+TGG | 0.537325 | 5.3:+6144807 | MS.gene09728:CDS |
| TGTAAGCGTTTCAGGTGGCG+GGG | 0.538613 | 5.3:-6143869 | None:intergenic |
| ACCGTTACCGGCCATAGCCT+TGG | 0.539736 | 5.3:+6144547 | MS.gene09728:CDS |
| ATGGCAGCAACGACTGGAAG+GGG | 0.541567 | 5.3:+6143960 | MS.gene09728:CDS |
| ACCTAAATGGGTTGATGAGG+TGG | 0.548455 | 5.3:+6144177 | MS.gene09728:CDS |
| ACTAGCCCTATTGGTGGCCG+AGG | 0.548502 | 5.3:+6144576 | MS.gene09728:CDS |
| ATTCATGAAGATGATGGACT+AGG | 0.548753 | 5.3:-6145159 | None:intergenic |
| AGGTAATCCAATTGATGATG+TGG | 0.548802 | 5.3:-6144158 | None:intergenic |
| GATATTGTGATATCTCTTCG+TGG | 0.549518 | 5.3:+6144295 | MS.gene09728:CDS |
| TCCAACCCAGCTAGAACGCT+GGG | 0.550701 | 5.3:-6144221 | None:intergenic |
| TTCAGCCCATTCAAGACAAG+TGG | 0.553127 | 5.3:-6144323 | None:intergenic |
| GGACGCTCAGACTCAGACTC+AGG | 0.556991 | 5.3:+6144378 | MS.gene09728:CDS |
| CGCCGGAGGTTGTCATCCAA+GGG | 0.557417 | 5.3:-6143992 | None:intergenic |
| ACAACAAGCTATGTCAGCAT+CGG | 0.563181 | 5.3:-6144908 | None:intergenic |
| CGGGCCAAACGTGCCACCCG+TGG | 0.563469 | 5.3:+6144612 | MS.gene09728:CDS |
| TCATGGCAGCAACGACTGGA+AGG | 0.564182 | 5.3:+6143958 | MS.gene09728:CDS |
| CGCCTTATTGAACTTTACAA+AGG | 0.564214 | 5.3:+6144508 | MS.gene09728:CDS |
| ATTCTCCAAGAAATATCATC+GGG | 0.566867 | 5.3:+6143918 | MS.gene09728:CDS |
| AGCTTGTTGTCATGATCTAG+AGG | 0.571065 | 5.3:+6144921 | MS.gene09728:CDS |
| GCCGAGGAAATTAGCACGTG+CGG | 0.571449 | 5.3:+6144592 | MS.gene09728:CDS |
| TGATGTGGCATACAGACTCT+TGG | 0.573169 | 5.3:-6144143 | None:intergenic |
| TAAGCGTTTCAGGTGGCGGG+GGG | 0.576225 | 5.3:-6143867 | None:intergenic |
| GGGTGCTTCAGTTGTTGACA+TGG | 0.576279 | 5.3:-6144077 | None:intergenic |
| CCGAGGAAATTAGCACGTGC+GGG | 0.576932 | 5.3:+6144593 | MS.gene09728:CDS |
| TCATTTGGCGGACCTCGTGT+TGG | 0.577348 | 5.3:+6144643 | MS.gene09728:CDS |
| CATATCTAACAACCTCTCGC+CGG | 0.581786 | 5.3:-6144009 | None:intergenic |
| CATGGCAGCAACGACTGGAA+GGG | 0.583943 | 5.3:+6143959 | MS.gene09728:CDS |
| AATTTGTGAAGGTTGTGAGC+AGG | 0.587076 | 5.3:-6143566 | None:intergenic |
| GCCAAGGCTATGGCCGGTAA+CGG | 0.591069 | 5.3:-6144548 | None:intergenic |
| TATTCTCCAAGAAATATCAT+CGG | 0.595846 | 5.3:+6143917 | MS.gene09728:CDS |
| AACGACACCTCTTGATGATG+GGG | 0.596360 | 5.3:-6144097 | None:intergenic |
| AGAAAGTGGAGGGAATATCA+TGG | 0.601338 | 5.3:+6143941 | MS.gene09728:CDS |
| TTGGTTGATGATGATTCCAA+AGG | 0.613659 | 5.3:-6143728 | None:intergenic |
| AGGTGGCACCAGATCTTGGT+TGG | 0.618491 | 5.3:+6144194 | MS.gene09728:CDS |
| GGATGACAACCTCCGGCGAG+AGG | 0.618831 | 5.3:+6143997 | MS.gene09728:CDS |
| CAGACTCAGACTCAGGCACA+AGG | 0.619166 | 5.3:+6144385 | MS.gene09728:CDS |
| TTAGTGCTTGAAATTTGTGA+AGG | 0.626013 | 5.3:-6143577 | None:intergenic |
| TCAAAGCCCAAAGTTGAGTG+TGG | 0.627127 | 5.3:+6144412 | MS.gene09728:CDS |
| ACAACAAGCAGCCATAGACA+TGG | 0.628855 | 5.3:-6145136 | None:intergenic |
| GCGTCGCACTTCTTCAACAA+CGG | 0.630718 | 5.3:-6144488 | None:intergenic |
| TTGTCTAATCTTAGTAGACA+GGG | 0.632883 | 5.3:+6145111 | MS.gene09728:CDS |
| ATCTAACAACCTCTCGCCGG+AGG | 0.634684 | 5.3:-6144006 | None:intergenic |
| TGAAGCACCCCATCATCAAG+AGG | 0.635362 | 5.3:+6144090 | MS.gene09728:CDS |
| TGTCGCGCCAAGGCTATGGC+CGG | 0.642253 | 5.3:-6144554 | None:intergenic |
| ACAGCGTCTTCTTTCCAAGA+CGG | 0.643880 | 5.3:+6143889 | MS.gene09728:CDS |
| TCTGTTAATCTTAATAGACA+AGG | 0.646433 | 5.3:+6145081 | MS.gene09728:CDS |
| TATCATCGGGAGAAAGTGGA+GGG | 0.648802 | 5.3:+6143931 | MS.gene09728:CDS |
| ATTACCTAAATGGGTTGATG+AGG | 0.663925 | 5.3:+6144174 | MS.gene09728:CDS |
| ATATCATCGGGAGAAAGTGG+AGG | 0.666029 | 5.3:+6143930 | MS.gene09728:CDS |
| ATAGGGCTAGTGTCGCGCCA+AGG | 0.667674 | 5.3:-6144564 | None:intergenic |
| CCAATTAGTCGATTTACCCG+AGG | 0.667868 | 5.3:+6144357 | MS.gene09728:CDS |
| GACGCTGTAAGCGTTTCAGG+TGG | 0.674999 | 5.3:-6143874 | None:intergenic |
| GCGTCTTCTTTCCAAGACGG+CGG | 0.680773 | 5.3:+6143892 | MS.gene09728:CDS |
| CCAACATGTGAATATCCCAA+AGG | 0.681313 | 5.3:-6144841 | None:intergenic |
| GAGCTTGTACAAGACAAAAG+GGG | 0.708452 | 5.3:+6144441 | MS.gene09728:CDS |
| CACACAAGACGTGATTACAA+GGG | 0.729372 | 5.3:+6144726 | MS.gene09728:CDS |
| AAGGCTCTGTTACCAACACG+AGG | 0.743509 | 5.3:-6144655 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAGTTGTATACTAGTA+AGG | + | chr5.3:6145047-6145066 | MS.gene09728:CDS | 20.0% |
| !! | AGAAAAAATTAAGAATTCCA+AGG | + | chr5.3:6144780-6144799 | MS.gene09728:CDS | 20.0% |
| ! | AAAAGATGAAGGTTGTTTAA+TGG | - | chr5.3:6143633-6143652 | None:intergenic | 25.0% |
| ! | AAAGGAAAAAACTTTCTTGA+AGG | + | chr5.3:6143794-6143813 | MS.gene09728:CDS | 25.0% |
| ! | ATCAATTGGATTACCTAAAT+GGG | + | chr5.3:6144165-6144184 | MS.gene09728:CDS | 25.0% |
| ! | ATCCAAAAAAGTGACAAAAA+AGG | + | chr5.3:6143776-6143795 | MS.gene09728:CDS | 25.0% |
| ! | TATTCTCCAAGAAATATCAT+CGG | + | chr5.3:6143917-6143936 | MS.gene09728:CDS | 25.0% |
| ! | TCTGTTAATCTTAATAGACA+AGG | + | chr5.3:6145081-6145100 | MS.gene09728:CDS | 25.0% |
| !! | GTTACTTTATAAGATCTTTC+CGG | - | chr5.3:6144124-6144143 | None:intergenic | 25.0% |
| !!! | TTCCTTTTTTGTCACTTTTT+TGG | - | chr5.3:6143781-6143800 | None:intergenic | 25.0% |
| !!! | TTTTTGTCACTTTTTTGGAT+AGG | - | chr5.3:6143776-6143795 | None:intergenic | 25.0% |
| AAAAGAGAGATTGCAAGAAT+GGG | + | chr5.3:6144265-6144284 | MS.gene09728:CDS | 30.0% | |
| AAACCAATAACAACTCATCT+AGG | + | chr5.3:6143663-6143682 | MS.gene09728:CDS | 30.0% | |
| AGAGGCTTATTTACATTTAG+TGG | + | chr5.3:6144939-6144958 | MS.gene09728:CDS | 30.0% | |
| ATAAATCCACACTCAACTTT+GGG | - | chr5.3:6144421-6144440 | None:intergenic | 30.0% | |
| ATTCTCCAAGAAATATCATC+GGG | + | chr5.3:6143918-6143937 | MS.gene09728:CDS | 30.0% | |
| CATCAATTGGATTACCTAAA+TGG | + | chr5.3:6144164-6144183 | MS.gene09728:CDS | 30.0% | |
| GCTTATTTACATTTAGTGGA+TGG | + | chr5.3:6144943-6144962 | MS.gene09728:CDS | 30.0% | |
| GTTGTTTAATGGAAGAAGAA+TGG | - | chr5.3:6143622-6143641 | None:intergenic | 30.0% | |
| TAAAAGAGAGATTGCAAGAA+TGG | + | chr5.3:6144264-6144283 | MS.gene09728:CDS | 30.0% | |
| TAACCTAGATGAGTTGTTAT+TGG | - | chr5.3:6143669-6143688 | None:intergenic | 30.0% | |
| TTAGTGCTTGAAATTTGTGA+AGG | - | chr5.3:6143580-6143599 | None:intergenic | 30.0% | |
| TTGTATACTAGTAAGGCTAA+AGG | + | chr5.3:6145054-6145073 | MS.gene09728:CDS | 30.0% | |
| TTGTCTAATCTTAGTAGACA+GGG | + | chr5.3:6145111-6145130 | MS.gene09728:CDS | 30.0% | |
| !!! | CTTTTTTGGATAGGTTGTAT+TGG | - | chr5.3:6143767-6143786 | None:intergenic | 30.0% |
| AACTTCCACTTGTCTTGAAT+GGG | + | chr5.3:6144318-6144337 | MS.gene09728:CDS | 35.0% | |
| AAGAATTCCAAGGTTGTTAG+TGG | + | chr5.3:6144790-6144809 | MS.gene09728:CDS | 35.0% | |
| ATGAGCTTGTACAAGACAAA+AGG | + | chr5.3:6144439-6144458 | MS.gene09728:CDS | 35.0% | |
| CATAAATCCACACTCAACTT+TGG | - | chr5.3:6144422-6144441 | None:intergenic | 35.0% | |
| CGCCTTTGTAAAGTTCAATA+AGG | - | chr5.3:6144513-6144532 | None:intergenic | 35.0% | |
| CTTGTCTAATCTTAGTAGAC+AGG | + | chr5.3:6145110-6145129 | MS.gene09728:CDS | 35.0% | |
| GAATTAAGCATAACCGTTAC+CGG | + | chr5.3:6144535-6144554 | MS.gene09728:CDS | 35.0% | |
| GACTTAACCTAGCAAAACTA+TGG | + | chr5.3:6143816-6143835 | MS.gene09728:CDS | 35.0% | |
| GATATTGTGATATCTCTTCG+TGG | + | chr5.3:6144295-6144314 | MS.gene09728:CDS | 35.0% | |
| GGTTGTTAGATATGGTGATT+TGG | + | chr5.3:6144018-6144037 | MS.gene09728:CDS | 35.0% | |
| TAGTGGAGTTGTTGATATGT+TGG | + | chr5.3:6144807-6144826 | MS.gene09728:CDS | 35.0% | |
| TCCTCGCTTAAAAATATTCC+TGG | - | chr5.3:6144754-6144773 | None:intergenic | 35.0% | |
| TGAAGAGAAGCAAAAGATGA+AGG | - | chr5.3:6143644-6143663 | None:intergenic | 35.0% | |
| TGAGCTTGTACAAGACAAAA+GGG | + | chr5.3:6144440-6144459 | MS.gene09728:CDS | 35.0% | |
| TTTCTCCCGATGATATTTCT+TGG | - | chr5.3:6143926-6143945 | None:intergenic | 35.0% | |
| ! | ACATTTAGTGGATGGATACA+TGG | + | chr5.3:6144951-6144970 | MS.gene09728:CDS | 35.0% |
| ! | ATGATGATTCCAAAGGCTTT+TGG | - | chr5.3:6143724-6143743 | None:intergenic | 35.0% |
| ! | CGCCTTATTGAACTTTACAA+AGG | + | chr5.3:6144508-6144527 | MS.gene09728:CDS | 35.0% |
| ! | TATTGGTTGTTGAAACTGGT+TGG | - | chr5.3:6143750-6143769 | None:intergenic | 35.0% |
| !! | AGGTAATCCAATTGATGATG+TGG | - | chr5.3:6144161-6144180 | None:intergenic | 35.0% |
| !! | ATTACCTAAATGGGTTGATG+AGG | + | chr5.3:6144174-6144193 | MS.gene09728:CDS | 35.0% |
| !! | CTCTTAGCATTAGCTCTAAA+TGG | - | chr5.3:6144988-6145007 | None:intergenic | 35.0% |
| !! | CTTTTCAAGATGTTCTCCAA+AGG | - | chr5.3:6144677-6144696 | None:intergenic | 35.0% |
| !! | GTTGTATTGGTTGTTGAAAC+TGG | - | chr5.3:6143754-6143773 | None:intergenic | 35.0% |
| !! | TTGGTTGATGATGATTCCAA+AGG | - | chr5.3:6143731-6143750 | None:intergenic | 35.0% |
| AACAACTCCACTAACAACCT+TGG | - | chr5.3:6144800-6144819 | None:intergenic | 40.0% | |
| AATTTGTGAAGGTTGTGAGC+AGG | - | chr5.3:6143569-6143588 | None:intergenic | 40.0% | |
| ACAACAAGCTATGTCAGCAT+CGG | - | chr5.3:6144911-6144930 | None:intergenic | 40.0% | |
| ACACACAAGACGTGATTACA+AGG | + | chr5.3:6144725-6144744 | MS.gene09728:CDS | 40.0% | |
| AGAAAGTGGAGGGAATATCA+TGG | + | chr5.3:6143941-6143960 | MS.gene09728:CDS | 40.0% | |
| CACACAAGACGTGATTACAA+GGG | + | chr5.3:6144726-6144745 | MS.gene09728:CDS | 40.0% | |
| CCAAATGAAAAAACAGCCAC+GGG | - | chr5.3:6144631-6144650 | None:intergenic | 40.0% | |
| CCAACATGTGAATATCCCAA+AGG | - | chr5.3:6144844-6144863 | None:intergenic | 40.0% | |
| CTCGGGTAAATCGACTAATT+GGG | - | chr5.3:6144359-6144378 | None:intergenic | 40.0% | |
| CTGTATGCCACATCATCAAT+TGG | + | chr5.3:6144151-6144170 | MS.gene09728:CDS | 40.0% | |
| GAAATATCATCGGGAGAAAG+TGG | + | chr5.3:6143927-6143946 | MS.gene09728:CDS | 40.0% | |
| GAACTTCCACTTGTCTTGAA+TGG | + | chr5.3:6144317-6144336 | MS.gene09728:CDS | 40.0% | |
| GAGCTTGTACAAGACAAAAG+GGG | + | chr5.3:6144441-6144460 | MS.gene09728:CDS | 40.0% | |
| GCCAAATGAAAAAACAGCCA+CGG | - | chr5.3:6144632-6144651 | None:intergenic | 40.0% | |
| TTGGAAGAGAACACTCCTTT+GGG | + | chr5.3:6144826-6144845 | MS.gene09728:CDS | 40.0% | |
| ! | ACTCAACTTTGGGCTTTGAT+TGG | - | chr5.3:6144411-6144430 | None:intergenic | 40.0% |
| ! | GAAGAATGGCATCTGAAACT+AGG | - | chr5.3:6143608-6143627 | None:intergenic | 40.0% |
| ! | TCTTCAGCTGCTTTAATCTC+TGG | - | chr5.3:6143842-6143861 | None:intergenic | 40.0% |
| ! | TCTTGATAGCCAAAAGCCTT+TGG | + | chr5.3:6143712-6143731 | MS.gene09728:CDS | 40.0% |
| !! | AGCTTGTTGTCATGATCTAG+AGG | + | chr5.3:6144921-6144940 | MS.gene09728:CDS | 40.0% |
| !! | CCTTTGGGATATTCACATGT+TGG | + | chr5.3:6144841-6144860 | MS.gene09728:CDS | 40.0% |
| !! | GCCAGGAATATTTTTAAGCG+AGG | + | chr5.3:6144750-6144769 | MS.gene09728:CDS | 40.0% |
| !!! | GTGGCTGTTTTTTCATTTGG+CGG | + | chr5.3:6144631-6144650 | MS.gene09728:CDS | 40.0% |
| AATATCATGGCAGCAACGAC+TGG | + | chr5.3:6143954-6143973 | MS.gene09728:CDS | 45.0% | |
| AATGAAAAAACAGCCACGGG+TGG | - | chr5.3:6144628-6144647 | None:intergenic | 45.0% | |
| AATTTCCTCGGCCACCAATA+GGG | - | chr5.3:6144584-6144603 | None:intergenic | 45.0% | |
| ACAACAAGCAGCCATAGACA+TGG | - | chr5.3:6145139-6145158 | None:intergenic | 45.0% | |
| ACAGCGTCTTCTTTCCAAGA+CGG | + | chr5.3:6143889-6143908 | MS.gene09728:CDS | 45.0% | |
| ATATCATCGGGAGAAAGTGG+AGG | + | chr5.3:6143930-6143949 | MS.gene09728:CDS | 45.0% | |
| CAACAAGCTATGTCAGCATC+GGG | - | chr5.3:6144910-6144929 | None:intergenic | 45.0% | |
| CATATCTAACAACCTCTCGC+CGG | - | chr5.3:6144012-6144031 | None:intergenic | 45.0% | |
| CCTCGGGTAAATCGACTAAT+TGG | - | chr5.3:6144360-6144379 | None:intergenic | 45.0% | |
| GTTGGAAGAGAACACTCCTT+TGG | + | chr5.3:6144825-6144844 | MS.gene09728:CDS | 45.0% | |
| TAATTTCCTCGGCCACCAAT+AGG | - | chr5.3:6144585-6144604 | None:intergenic | 45.0% | |
| TAGACAGGGATCCATGTCTA+TGG | + | chr5.3:6145125-6145144 | MS.gene09728:CDS | 45.0% | |
| TATCATCGGGAGAAAGTGGA+GGG | + | chr5.3:6143931-6143950 | MS.gene09728:CDS | 45.0% | |
| TCAAAGCCCAAAGTTGAGTG+TGG | + | chr5.3:6144412-6144431 | MS.gene09728:CDS | 45.0% | |
| TGATGTGGCATACAGACTCT+TGG | - | chr5.3:6144146-6144165 | None:intergenic | 45.0% | |
| TTCAGCCCATTCAAGACAAG+TGG | - | chr5.3:6144326-6144345 | None:intergenic | 45.0% | |
| ! | CAACGACACCTCTTGATGAT+GGG | - | chr5.3:6144101-6144120 | None:intergenic | 45.0% |
| ! | CACTTCTTCAACAACGGACT+CGG | - | chr5.3:6144485-6144504 | None:intergenic | 45.0% |
| ! | CCAATTAGTCGATTTACCCG+AGG | + | chr5.3:6144357-6144376 | MS.gene09728:CDS | 45.0% |
| !! | AACGACACCTCTTGATGATG+GGG | - | chr5.3:6144100-6144119 | None:intergenic | 45.0% |
| !! | ACCTAAATGGGTTGATGAGG+TGG | + | chr5.3:6144177-6144196 | MS.gene09728:CDS | 45.0% |
| !! | TCTCTGGCCATAGTTTTGCT+AGG | - | chr5.3:6143826-6143845 | None:intergenic | 45.0% |
| !!! | CCCGTGGCTGTTTTTTCATT+TGG | + | chr5.3:6144628-6144647 | MS.gene09728:CDS | 45.0% |
| AAGGCTCTGTTACCAACACG+AGG | - | chr5.3:6144658-6144677 | None:intergenic | 50.0% | |
| ATCCCTTGGATGACAACCTC+CGG | + | chr5.3:6143990-6144009 | MS.gene09728:CDS | 50.0% | |
| CGGCGAGAGGTTGTTAGATA+TGG | + | chr5.3:6144010-6144029 | MS.gene09728:CDS | 50.0% | |
| GAAGACGCTGTAAGCGTTTC+AGG | - | chr5.3:6143880-6143899 | None:intergenic | 50.0% | |
| GAAGGGGATGTTAGATCCCT+TGG | + | chr5.3:6143976-6143995 | MS.gene09728:CDS | 50.0% | |
| GCAACGACACCTCTTGATGA+TGG | - | chr5.3:6144102-6144121 | None:intergenic | 50.0% | |
| GCCACCTCATCAACCCATTT+AGG | - | chr5.3:6144181-6144200 | None:intergenic | 50.0% | |
| GCGTCGCACTTCTTCAACAA+CGG | - | chr5.3:6144491-6144510 | None:intergenic | 50.0% | |
| GCTTCAGTTGTTGACATGGC+AGG | - | chr5.3:6144076-6144095 | None:intergenic | 50.0% | |
| GGGTGCTTCAGTTGTTGACA+TGG | - | chr5.3:6144080-6144099 | None:intergenic | 50.0% | |
| GTGAAGGTTGTGAGCAGGTA+AGG | - | chr5.3:6143564-6143583 | None:intergenic | 50.0% | |
| TGGGTCATCCAACCAAGATC+TGG | - | chr5.3:6144205-6144224 | None:intergenic | 50.0% | |
| ! | CTTCAACAACGGACTCGGAT+AGG | - | chr5.3:6144480-6144499 | None:intergenic | 50.0% |
| ! | TCAGCATCGGGCTTCAAGTA+AGG | - | chr5.3:6144898-6144917 | None:intergenic | 50.0% |
| ! | TGAAGCACCCCATCATCAAG+AGG | + | chr5.3:6144090-6144109 | MS.gene09728:CDS | 50.0% |
| !! | CGTGTTGGTAACAGAGCCTT+TGG | + | chr5.3:6144658-6144677 | MS.gene09728:CDS | 50.0% |
| !! | TCATCAAGAGGTGTCGTTGC+CGG | + | chr5.3:6144102-6144121 | MS.gene09728:CDS | 50.0% |
| AGGTGGCACCAGATCTTGGT+TGG | + | chr5.3:6144194-6144213 | MS.gene09728:CDS | 55.0% | |
| AGTCTGAGTCTGAGCGTCCT+CGG | - | chr5.3:6144377-6144396 | None:intergenic | 55.0% | |
| ATCCAACCCAGCTAGAACGC+TGG | - | chr5.3:6144225-6144244 | None:intergenic | 55.0% | |
| ATCTAACAACCTCTCGCCGG+AGG | - | chr5.3:6144009-6144028 | None:intergenic | 55.0% | |
| ATGGCAGCAACGACTGGAAG+GGG | + | chr5.3:6143960-6143979 | MS.gene09728:CDS | 55.0% | |
| CAGACTCAGACTCAGGCACA+AGG | + | chr5.3:6144385-6144404 | MS.gene09728:CDS | 55.0% | |
| CATGGCAGCAACGACTGGAA+GGG | + | chr5.3:6143959-6143978 | MS.gene09728:CDS | 55.0% | |
| CCCGCACGTGCTAATTTCCT+CGG | - | chr5.3:6144596-6144615 | None:intergenic | 55.0% | |
| CCGAGGAAATTAGCACGTGC+GGG | + | chr5.3:6144593-6144612 | MS.gene09728:CDS | 55.0% | |
| GACGCTGTAAGCGTTTCAGG+TGG | - | chr5.3:6143877-6143896 | None:intergenic | 55.0% | |
| GATGAGGTGGCACCAGATCT+TGG | + | chr5.3:6144190-6144209 | MS.gene09728:CDS | 55.0% | |
| GCCGAGGAAATTAGCACGTG+CGG | + | chr5.3:6144592-6144611 | MS.gene09728:CDS | 55.0% | |
| GCGTCTTCTTTCCAAGACGG+CGG | + | chr5.3:6143892-6143911 | MS.gene09728:CDS | 55.0% | |
| TCATGGCAGCAACGACTGGA+AGG | + | chr5.3:6143958-6143977 | MS.gene09728:CDS | 55.0% | |
| TCCAACCCAGCTAGAACGCT+GGG | - | chr5.3:6144224-6144243 | None:intergenic | 55.0% | |
| TGGAGAATACTCCGCCGTCT+TGG | - | chr5.3:6143906-6143925 | None:intergenic | 55.0% | |
| ! | CTGTAAGCGTTTCAGGTGGC+GGG | - | chr5.3:6143873-6143892 | None:intergenic | 55.0% |
| ! | GATGACCCAGCGTTCTAGCT+GGG | + | chr5.3:6144216-6144235 | MS.gene09728:CDS | 55.0% |
| ! | GCTGTAAGCGTTTCAGGTGG+CGG | - | chr5.3:6143874-6143893 | None:intergenic | 55.0% |
| ! | TCATTTGGCGGACCTCGTGT+TGG | + | chr5.3:6144643-6144662 | MS.gene09728:CDS | 55.0% |
| ! | TGTAAGCGTTTCAGGTGGCG+GGG | - | chr5.3:6143872-6143891 | None:intergenic | 55.0% |
| !! | GACGTGATTACAAGGGTGCC+AGG | + | chr5.3:6144733-6144752 | MS.gene09728:CDS | 55.0% |
| ACCGTTACCGGCCATAGCCT+TGG | + | chr5.3:6144547-6144566 | MS.gene09728:CDS | 60.0% | |
| CGCCGGAGGTTGTCATCCAA+GGG | - | chr5.3:6143995-6144014 | None:intergenic | 60.0% | |
| CGCGACACTAGCCCTATTGG+TGG | + | chr5.3:6144570-6144589 | MS.gene09728:CDS | 60.0% | |
| CTAGTGTCGCGCCAAGGCTA+TGG | - | chr5.3:6144561-6144580 | None:intergenic | 60.0% | |
| GCCAAGGCTATGGCCGGTAA+CGG | - | chr5.3:6144551-6144570 | None:intergenic | 60.0% | |
| GGACGCTCAGACTCAGACTC+AGG | + | chr5.3:6144378-6144397 | MS.gene09728:CDS | 60.0% | |
| GGATGACCCAGCGTTCTAGC+TGG | + | chr5.3:6144215-6144234 | MS.gene09728:CDS | 60.0% | |
| GTCTGAGTCTGAGCGTCCTC+GGG | - | chr5.3:6144376-6144395 | None:intergenic | 60.0% | |
| TCGCCGGAGGTTGTCATCCA+AGG | - | chr5.3:6143996-6144015 | None:intergenic | 60.0% | |
| TGGCGCGACACTAGCCCTAT+TGG | + | chr5.3:6144567-6144586 | MS.gene09728:CDS | 60.0% | |
| ! | ACCCAGCGTTCTAGCTGGGT+TGG | + | chr5.3:6144220-6144239 | MS.gene09728:CDS | 60.0% |
| ! | ACTAGCCCTATTGGTGGCCG+AGG | + | chr5.3:6144576-6144595 | MS.gene09728:CDS | 60.0% |
| ! | ATAGGGCTAGTGTCGCGCCA+AGG | - | chr5.3:6144567-6144586 | None:intergenic | 60.0% |
| ! | GTAAGCGTTTCAGGTGGCGG+GGG | - | chr5.3:6143871-6143890 | None:intergenic | 60.0% |
| !! | TAAGCGTTTCAGGTGGCGGG+GGG | - | chr5.3:6143870-6143889 | None:intergenic | 60.0% |
| ACAGCCACGGGTGGCACGTT+TGG | - | chr5.3:6144619-6144638 | None:intergenic | 65.0% | |
| GGATGACAACCTCCGGCGAG+AGG | + | chr5.3:6143997-6144016 | MS.gene09728:CDS | 65.0% | |
| TGTCGCGCCAAGGCTATGGC+CGG | - | chr5.3:6144557-6144576 | None:intergenic | 65.0% | |
| CGGGCCAAACGTGCCACCCG+TGG | + | chr5.3:6144612-6144631 | MS.gene09728:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 6143539 | 6145179 | 6143539 | ID=MS.gene09728 |
| chr5.3 | mRNA | 6143539 | 6145179 | 6143539 | ID=MS.gene09728.t1;Parent=MS.gene09728 |
| chr5.3 | exon | 6143539 | 6145179 | 6143539 | ID=MS.gene09728.t1.exon1;Parent=MS.gene09728.t1 |
| chr5.3 | CDS | 6143539 | 6145179 | 6143539 | ID=cds.MS.gene09728.t1;Parent=MS.gene09728.t1 |
| Gene Sequence |
| Protein sequence |
