AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20311

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20311.t1 MTR_5g015210 99.335 451 3 0 1 451 1 451 0.0 909
MS.gene20311.t1 MTR_1g017770 62.899 407 145 4 51 451 28 434 4.92e-175 497
MS.gene20311.t1 MTR_8g092870 30.653 398 248 10 63 434 5 400 3.91e-39 146
MS.gene20311.t1 MTR_3g095730 29.383 405 254 8 58 434 1 401 2.96e-35 135
MS.gene20311.t1 MTR_4g085720 28.535 389 264 9 55 430 1 388 4.60e-35 135
MS.gene20311.t1 MTR_5g077510 29.925 401 226 11 67 430 35 417 2.68e-33 130
MS.gene20311.t1 MTR_6g071340 28.784 403 236 10 67 430 5 395 5.51e-33 129
MS.gene20311.t1 MTR_5g048050 29.529 403 233 11 67 430 25 415 3.32e-32 127
MS.gene20311.t1 MTR_7g106340 27.500 400 263 8 66 438 7 406 4.40e-31 124
MS.gene20311.t1 MTR_2g087350 28.389 391 258 9 66 438 5 391 3.99e-29 118
MS.gene20311.t1 MTR_4g063800 27.049 366 245 7 67 417 255 613 8.95e-29 119
MS.gene20311.t1 MTR_3g065080 26.615 387 256 7 67 430 36 417 1.08e-27 114
MS.gene20311.t1 MTR_1g094215 27.193 342 218 6 67 388 64 394 3.16e-27 113
MS.gene20311.t1 MTR_1g094215 27.193 342 218 6 67 388 247 577 1.94e-26 112
MS.gene20311.t1 MTR_7g059405 27.177 379 250 11 67 430 264 631 3.84e-26 112
MS.gene20311.t1 MTR_4g485520 27.195 353 228 11 67 399 105 448 8.00e-26 110
MS.gene20311.t1 MTR_4g485520 27.195 353 228 11 67 399 273 616 1.56e-25 110
MS.gene20311.t1 MTR_7g078330 25.916 382 240 8 67 425 274 635 1.71e-24 107
MS.gene20311.t1 MTR_3g096370 27.577 388 252 11 67 430 10 392 8.25e-24 103
MS.gene20311.t1 MTR_1g056910 26.667 405 233 14 66 429 5 386 3.07e-23 101
MS.gene20311.t1 MTR_1g056870 25.659 417 266 9 66 438 6 422 5.23e-23 101
MS.gene20311.t1 MTR_1g056880 25.659 417 266 9 66 438 6 422 1.95e-22 99.8
MS.gene20311.t1 MTR_1g056840 25.240 416 243 10 66 438 6 396 1.02e-21 97.1
MS.gene20311.t1 MTR_5g083030 25.000 356 227 6 67 389 293 641 6.24e-21 96.3
MS.gene20311.t1 MTR_4g051515 23.040 421 271 12 66 448 5 410 9.20e-21 94.4
MS.gene20311.t1 MTR_4g107010 27.273 363 232 12 52 388 264 620 3.32e-20 94.0
MS.gene20311.t1 MTR_7g116600 26.590 346 213 10 68 388 79 408 1.28e-19 91.3
MS.gene20311.t1 MTR_2g018010 25.887 282 186 5 67 327 292 571 1.26e-18 89.0
MS.gene20311.t1 MTR_7g053260 25.588 340 223 11 68 388 79 407 6.42e-18 86.3
MS.gene20311.t1 MTR_3g078160 26.316 342 226 8 68 388 276 612 1.23e-17 85.9
MS.gene20311.t1 MTR_3g078160 26.316 342 226 8 68 388 276 612 1.31e-17 85.9
MS.gene20311.t1 MTR_5g034440 27.893 337 211 10 38 351 255 582 1.59e-17 85.5
MS.gene20311.t1 MTR_2g011140 25.455 275 188 5 73 337 21 288 1.48e-16 81.6
MS.gene20311.t1 MTR_1g079450 26.766 269 177 8 67 320 63 326 7.72e-16 79.7
MS.gene20311.t1 MTR_3g085610 50.000 72 36 0 68 139 281 352 8.83e-16 80.5
MS.gene20311.t1 MTR_4g028960 25.292 257 158 7 60 287 274 525 4.09e-15 78.2
MS.gene20311.t1 MTR_1g069845 48.485 66 34 0 68 133 125 190 3.35e-14 75.1
MS.gene20311.t1 MTR_1g069845 48.485 66 34 0 68 133 246 311 5.17e-14 74.7
MS.gene20311.t1 MTR_1g069845 48.485 66 34 0 68 133 242 307 5.25e-14 74.7
MS.gene20311.t1 MTR_3g466220 44.578 83 46 0 51 133 217 299 6.13e-14 74.7
MS.gene20311.t1 MTR_3g466220 44.578 83 46 0 51 133 196 278 6.49e-14 74.7
MS.gene20311.t1 MTR_3g078160 26.809 235 156 5 68 286 276 510 6.49e-14 74.3
MS.gene20311.t1 MTR_4g091880 27.190 331 196 10 66 388 4 297 9.81e-14 72.8
MS.gene20311.t1 MTR_4g091880 32.847 137 79 4 66 197 4 132 2.67e-13 71.6
MS.gene20311.t1 MTR_8g077205 46.970 66 35 0 68 133 276 341 4.88e-13 71.6
MS.gene20311.t1 MTR_8g077205 46.970 66 35 0 68 133 164 229 5.25e-13 71.6
MS.gene20311.t1 MTR_1g093965 41.892 74 43 0 80 153 1 74 1.30e-12 67.0
MS.gene20311.t1 MTR_1g093995 41.892 74 43 0 80 153 1 74 1.53e-12 66.6
MS.gene20311.t1 MTR_8g080280 45.714 70 38 0 68 137 283 352 2.84e-12 69.3
MS.gene20311.t1 MTR_8g103227 47.761 67 34 1 68 133 396 462 3.05e-12 69.3
MS.gene20311.t1 MTR_3g115670 29.921 127 81 2 45 163 8 134 3.64e-12 68.9
MS.gene20311.t1 MTR_1g090320 41.237 97 54 2 68 162 514 609 4.09e-12 69.3
MS.gene20311.t1 MTR_1g090320 41.237 97 54 2 68 162 516 611 4.16e-12 68.9
MS.gene20311.t1 MTR_7g097020 44.776 67 37 0 67 133 241 307 7.03e-12 68.2
MS.gene20311.t1 MTR_7g005940 35.398 113 54 3 67 161 595 706 2.58e-11 66.6
MS.gene20311.t1 MTR_7g005940 35.398 113 54 3 67 161 595 706 2.86e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20311.t1 AT3G49810 74.058 451 114 1 1 451 1 448 0.0 669
MS.gene20311.t1 AT5G65920 71.397 451 122 1 1 451 1 444 0.0 649
MS.gene20311.t1 AT5G65920 71.397 451 122 1 1 451 1 444 0.0 649
MS.gene20311.t1 AT3G19380 30.105 382 242 11 57 416 3 381 5.91e-40 149
MS.gene20311.t1 AT1G49780 29.484 407 261 9 66 449 13 416 7.45e-37 140
MS.gene20311.t1 AT2G35930 26.368 402 269 9 63 443 8 403 3.32e-34 132
MS.gene20311.t1 AT5G64660 29.136 405 247 14 63 434 5 402 2.85e-33 130
MS.gene20311.t1 AT3G18710 28.244 393 255 10 67 436 12 400 1.30e-32 128
MS.gene20311.t1 AT3G54850 27.344 384 247 10 67 430 248 619 7.67e-31 125
MS.gene20311.t1 AT5G09800 26.794 418 259 13 60 447 4 404 9.45e-30 120
MS.gene20311.t1 AT1G23030 27.616 344 229 8 67 398 241 576 4.65e-29 120
MS.gene20311.t1 AT1G66160 26.753 385 260 8 60 430 27 403 1.31e-27 114
MS.gene20311.t1 AT1G71020 25.714 350 237 7 67 398 95 439 1.68e-25 108
MS.gene20311.t1 AT3G46510 25.195 385 257 7 67 432 256 628 2.38e-25 109
MS.gene20311.t1 AT3G52450 25.598 418 262 10 58 430 1 414 2.60e-25 108
MS.gene20311.t1 AT1G71020 25.714 350 237 7 67 398 243 587 2.87e-25 109
MS.gene20311.t1 AT5G42340 29.339 242 147 5 67 302 290 513 5.74e-25 108
MS.gene20311.t1 AT2G28830 25.588 340 229 6 68 388 185 519 1.51e-23 103
MS.gene20311.t1 AT5G37490 23.684 380 274 5 67 430 31 410 6.80e-20 92.0
MS.gene20311.t1 AT1G66160 24.675 385 252 9 60 430 27 387 7.32e-19 89.0
MS.gene20311.t1 AT1G29340 30.978 184 108 3 67 232 305 487 4.51e-18 87.4
MS.gene20311.t1 AT3G07360 29.004 231 138 8 55 261 57 285 1.60e-16 82.0
MS.gene20311.t1 AT5G01830 27.143 280 184 6 22 286 230 504 1.73e-16 82.4
MS.gene20311.t1 AT3G11840 53.247 77 34 2 58 133 1 76 8.34e-16 79.7
MS.gene20311.t1 AT4G21350 26.567 335 222 10 66 388 4 326 9.37e-16 79.0
MS.gene20311.t1 AT1G67530 51.429 70 34 0 68 137 273 342 9.88e-15 77.0
MS.gene20311.t1 AT1G67530 51.429 70 34 0 68 137 273 342 9.88e-15 77.0
MS.gene20311.t1 AT1G67530 51.429 70 34 0 68 137 273 342 9.88e-15 77.0
MS.gene20311.t1 AT1G67530 51.429 70 34 0 68 137 273 342 1.05e-14 76.6
MS.gene20311.t1 AT1G24330 50.725 69 34 0 68 136 276 344 4.36e-14 75.1
MS.gene20311.t1 AT1G24330 50.725 69 34 0 68 136 276 344 4.36e-14 75.1
MS.gene20311.t1 AT1G10560 24.679 312 200 8 71 355 292 595 4.91e-14 74.7
MS.gene20311.t1 AT1G27910 50.000 70 35 0 68 137 280 349 7.08e-14 74.3
MS.gene20311.t1 AT1G60190 23.754 341 230 10 73 388 284 619 1.57e-13 73.2
MS.gene20311.t1 AT5G67340 46.269 67 36 0 67 133 240 306 2.48e-12 69.7
MS.gene20311.t1 AT5G67340 46.269 67 36 0 67 133 240 306 2.48e-12 69.7
MS.gene20311.t1 AT3G54790 41.176 68 40 0 67 134 202 269 4.69e-12 68.6
MS.gene20311.t1 AT2G23140 46.032 63 34 0 71 133 237 299 4.88e-12 68.6
MS.gene20311.t1 AT2G23140 46.032 63 34 0 71 133 234 296 5.04e-12 68.6
MS.gene20311.t1 AT2G23140 46.032 63 34 0 71 133 234 296 5.04e-12 68.6
MS.gene20311.t1 AT2G23140 46.032 63 34 0 71 133 45 107 5.10e-12 68.6
MS.gene20311.t1 AT2G23140 46.032 63 34 0 71 133 45 107 5.10e-12 68.6
MS.gene20311.t1 AT3G54790 41.176 68 40 0 67 134 238 305 5.83e-12 68.6
MS.gene20311.t1 AT3G54790 41.176 68 40 0 67 134 238 305 5.83e-12 68.6
MS.gene20311.t1 AT5G65500 43.056 72 41 0 66 137 714 785 3.88e-11 65.9
MS.gene20311.t1 AT5G65500 43.056 72 41 0 66 137 722 793 4.07e-11 65.9

Find 98 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTCCAGCCATAGTTTAT+TGG 0.212618 5.1:-7828276 MS.gene20311:CDS
GCAGATTGTTGTTTCAATAT+TGG 0.233260 5.1:+7827837 None:intergenic
GGAATTAGCCCTTTGTGTTT+TGG 0.251271 5.1:-7828084 MS.gene20311:CDS
TGCATTGGTTCAAGAGAAAT+TGG 0.251419 5.1:+7828869 None:intergenic
CTCCAAACACAACACTTTAT+AGG 0.268814 5.1:-7828727 MS.gene20311:CDS
CTATGTATCAGCCTTCTAAA+AGG 0.293724 5.1:-7829084 MS.gene20311:CDS
TGTCCAGTGCAAAGAGTAAC+AGG 0.310547 5.1:+7828842 None:intergenic
TACAACTATGCAAGAGCTTT+GGG 0.339571 5.1:-7828762 MS.gene20311:CDS
CCAACAGCATGTGAAGTAAA+AGG 0.340191 5.1:+7828482 None:intergenic
CAGAACAGTGTGCTTCTATA+AGG 0.348100 5.1:-7828186 MS.gene20311:CDS
ACCTGCAAAGATTTCTTTGA+TGG 0.353869 5.1:-7828393 MS.gene20311:CDS
GAGAATGTTGTTCCTCTCAT+AGG 0.363619 5.1:+7828817 None:intergenic
TCAATCCAACCAATAAACTA+TGG 0.369818 5.1:+7828267 None:intergenic
GCTGTTGGTTCTGAAGTTAT+TGG 0.374377 5.1:-7828467 MS.gene20311:CDS
GGGTATGAGGGTGGTGGTGA+TGG 0.376806 5.1:-7829049 MS.gene20311:CDS
TCGTCGATTGCCGTTGATGC+AGG 0.377688 5.1:-7827903 MS.gene20311:CDS
GTTGATTGTGGTGGTATTGT+AGG 0.378694 5.1:-7828977 MS.gene20311:CDS
TTCAAAGCTACCCTGCCTTC+AGG 0.379046 5.1:+7828044 None:intergenic
AACCTATAAAGTGTTGTGTT+TGG 0.379505 5.1:+7828725 None:intergenic
ACCATCAAAGAAATCTTTGC+AGG 0.386948 5.1:+7828392 None:intergenic
AGCTGTGAAAGATGGGGTTT+TGG 0.389850 5.1:-7829005 MS.gene20311:CDS
CAAAGCTCTTGCATAGTTGT+AGG 0.392894 5.1:+7828764 None:intergenic
AGCTAGAGTTCATGCTTTGA+AGG 0.394200 5.1:-7828594 MS.gene20311:CDS
CAATTAGTTGAATTGTTGTC+TGG 0.396249 5.1:-7828125 MS.gene20311:CDS
TCAGCCTTCTAAAAGGGATA+TGG 0.401063 5.1:-7829077 MS.gene20311:CDS
CTACAACTATGCAAGAGCTT+TGG 0.413732 5.1:-7828763 MS.gene20311:CDS
ATTGTAGGAACTGGTGTTGT+TGG 0.418341 5.1:-7828962 MS.gene20311:CDS
TGAAGGCAGGGTAGCTTTGA+AGG 0.418617 5.1:-7828042 MS.gene20311:CDS
GGATATGGTTGTTGGGTATG+AGG 0.419887 5.1:-7829062 MS.gene20311:CDS
AGTTGAATTGTTGTCTGGTA+TGG 0.429978 5.1:-7828120 MS.gene20311:CDS
CCAGTGTCATTGGATGGTTC+TGG 0.433464 5.1:+7827749 None:intergenic
TCTAAAAGGGATATGGTTGT+TGG 0.433712 5.1:-7829070 MS.gene20311:CDS
GGAGACACTCAAGAAGGTTA+AGG 0.438920 5.1:-7828621 MS.gene20311:CDS
ATCAAAAGTCTCACCATGAT+AGG 0.446610 5.1:+7828002 None:intergenic
AGCGGCAAGCCCTGCATCAA+CGG 0.453178 5.1:+7827893 None:intergenic
TCCAACCAATAAACTATGGC+TGG 0.454386 5.1:+7828271 None:intergenic
TGGTATGGACCATGATTGTT+TGG 0.458739 5.1:-7828105 MS.gene20311:CDS
TGATGGTCAGATTCTAGATC+TGG 0.470143 5.1:-7829032 MS.gene20311:CDS
CTGGACACAGCTGTGAAAGA+TGG 0.472189 5.1:-7829013 MS.gene20311:CDS
CTAAAAGGGATATGGTTGTT+GGG 0.472206 5.1:-7829069 MS.gene20311:CDS
TGGATTGATGAGGCTTGTTA+AGG 0.477377 5.1:-7828252 MS.gene20311:CDS
CATTCTCAAATGGTTTAACA+TGG 0.477905 5.1:-7828801 MS.gene20311:CDS
AAGTGTTGTGTTTGGAGTGA+TGG 0.479659 5.1:+7828733 None:intergenic
CGTCGATTGCCGTTGATGCA+GGG 0.480126 5.1:-7827902 MS.gene20311:CDS
TGGACACAGCTGTGAAAGAT+GGG 0.480846 5.1:-7829012 MS.gene20311:CDS
TCCAGCCATAGTTTATTGGT+TGG 0.483049 5.1:-7828272 MS.gene20311:CDS
TTTATAGGTTAATATATACT+TGG 0.486014 5.1:-7828712 MS.gene20311:CDS
AGAGGAACAACATTCTCAAA+TGG 0.486614 5.1:-7828811 MS.gene20311:CDS
GCACGTAAGTCTGTGATTGA+TGG 0.491037 5.1:-7828539 MS.gene20311:CDS
TTGGGTGGTGTTGATTGTGG+TGG 0.491523 5.1:-7828986 MS.gene20311:CDS
TGTTAACACCATACCTATCA+TGG 0.494074 5.1:-7828015 MS.gene20311:CDS
CATAGCAATGGAATCTGTCC+TGG 0.495678 5.1:-7828221 MS.gene20311:CDS
GGCAATCGACGAACACTCCT+CGG 0.495695 5.1:+7827914 None:intergenic
AGGAAGAGCTTCTGAGCTTG+TGG 0.496986 5.1:-7828642 MS.gene20311:CDS
ATGGTTGTTGGGTATGAGGG+TGG 0.497015 5.1:-7829058 MS.gene20311:CDS
TGTGGTGGTATTGTAGGAAC+TGG 0.497597 5.1:-7828971 MS.gene20311:CDS
GTTGATTGAAACGTTAATTG+AGG 0.502694 5.1:-7828321 MS.gene20311:CDS
GAGACACTCAAGAAGGTTAA+GGG 0.509193 5.1:-7828620 MS.gene20311:CDS
GATATGGTTGTTGGGTATGA+GGG 0.510561 5.1:-7829061 MS.gene20311:CDS
TATGTATCAGCCTTCTAAAA+GGG 0.514271 5.1:-7829083 MS.gene20311:CDS
CTTACACTTGGAAATGAAGA+TGG 0.515200 5.1:+7827776 None:intergenic
GATGGTTGATATCTTGAATG+AGG 0.519499 5.1:-7828375 MS.gene20311:CDS
CTCCTTCTTGTGATTCAAAG+TGG 0.522084 5.1:-7827867 MS.gene20311:CDS
TATCGTCAATACCTGAAGGC+AGG 0.524187 5.1:-7828055 MS.gene20311:CDS
GACCCTGTTACTCTTTGCAC+TGG 0.524861 5.1:-7828845 MS.gene20311:CDS
GAAGGAGCTTCATCAAGTTG+TGG 0.525660 5.1:-7828576 MS.gene20311:CDS
AAGAGGTCTGAAGATGTTCA+AGG 0.532675 5.1:-7828662 MS.gene20311:CDS
GTTGTTGGGTATGAGGGTGG+TGG 0.533466 5.1:-7829055 MS.gene20311:CDS
TTTAATCCAGTGTCATTGGA+TGG 0.544882 5.1:+7827743 None:intergenic
GCAATCGACGAACACTCCTC+GGG 0.548555 5.1:+7827915 None:intergenic
GGGCTAATTCCAAACAATCA+TGG 0.556734 5.1:+7828096 None:intergenic
AGAGTAACAGGGTCTTGCAT+TGG 0.556765 5.1:+7828854 None:intergenic
TAACGAATCCAAAACACAAA+GGG 0.557474 5.1:+7828076 None:intergenic
AAGTCTCACCATGATAGGTA+TGG 0.557498 5.1:+7828007 None:intergenic
GTTTATTGGTTGGATTGATG+AGG 0.566504 5.1:-7828262 MS.gene20311:CDS
GGTTCTGGTAAGCTTACACT+TGG 0.573632 5.1:+7827764 None:intergenic
AGTACTTGTTGATGAAGAAG+AGG 0.579589 5.1:-7828679 MS.gene20311:CDS
TGTTTGCAAGCTCGCACCCG+AGG 0.590908 5.1:-7827931 MS.gene20311:CDS
AATCACAAGAAGGAGCTTAG+CGG 0.593465 5.1:+7827875 None:intergenic
ATGGTTGATATCTTGAATGA+GGG 0.598018 5.1:-7828374 MS.gene20311:CDS
TATAGAAGCACACTGTTCTG+AGG 0.600791 5.1:+7828189 None:intergenic
ATCGTCAATACCTGAAGGCA+GGG 0.601834 5.1:-7828054 MS.gene20311:CDS
AACCACTTTGAATCACAAGA+AGG 0.602714 5.1:+7827865 None:intergenic
GTTCTGAGGAGGCTTAATCC+AGG 0.612490 5.1:+7828203 None:intergenic
ATTCTCAAATGGTTTAACAT+GGG 0.615647 5.1:-7828800 MS.gene20311:CDS
AGAAGCACACTGTTCTGAGG+AGG 0.626107 5.1:+7828192 None:intergenic
ATTGGACAAATGAAAACACT+TGG 0.634832 5.1:+7828887 None:intergenic
TCGTTATCGTCAATACCTGA+AGG 0.636371 5.1:-7828059 MS.gene20311:CDS
ACTATGGCTGGAGATAATCT+CGG 0.640439 5.1:+7828283 None:intergenic
AAGTCTGTGATTGATGGAGG+TGG 0.645793 5.1:-7828533 MS.gene20311:CDS
CGTAAGTCTGTGATTGATGG+AGG 0.663245 5.1:-7828536 MS.gene20311:CDS
GCTTGTGGAGACACTCAAGA+AGG 0.663911 5.1:-7828627 MS.gene20311:CDS
AAGGATAAGAGACATAGCAA+TGG 0.668679 5.1:-7828233 MS.gene20311:CDS
GCACTGGACAAACCTATGAG+AGG 0.669601 5.1:-7828829 MS.gene20311:CDS
ATAACGAATCCAAAACACAA+AGG 0.671691 5.1:+7828075 None:intergenic
GGACACAGCTGTGAAAGATG+GGG 0.688951 5.1:-7829011 MS.gene20311:CDS
AGACTAAAATCAGTTGCACG+CGG 0.721441 5.1:-7828343 MS.gene20311:CDS
GTCCAGTGCAAAGAGTAACA+GGG 0.727176 5.1:+7828843 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATAGGTTAATATATACT+TGG - chr5.1:7828131-7828150 MS.gene20311:CDS 15.0%
! ATTCTCAAATGGTTTAACAT+GGG - chr5.1:7828043-7828062 MS.gene20311:CDS 25.0%
!! AAGATTTTGTTAGTTAGCAT+TGG - chr5.1:7828685-7828704 MS.gene20311:CDS 25.0%
ATAACGAATCCAAAACACAA+AGG + chr5.1:7828771-7828790 None:intergenic 30.0%
ATTGGACAAATGAAAACACT+TGG + chr5.1:7827959-7827978 None:intergenic 30.0%
CAATTAGTTGAATTGTTGTC+TGG - chr5.1:7828718-7828737 MS.gene20311:CDS 30.0%
CATTCTCAAATGGTTTAACA+TGG - chr5.1:7828042-7828061 MS.gene20311:CDS 30.0%
GTTGATTGAAACGTTAATTG+AGG - chr5.1:7828522-7828541 MS.gene20311:CDS 30.0%
TAACGAATCCAAAACACAAA+GGG + chr5.1:7828770-7828789 None:intergenic 30.0%
TCAATCCAACCAATAAACTA+TGG + chr5.1:7828579-7828598 None:intergenic 30.0%
! AGTATGCATTGTCAATTTTG+TGG - chr5.1:7828887-7828906 MS.gene20311:CDS 30.0%
! ATGGTTGATATCTTGAATGA+GGG - chr5.1:7828469-7828488 MS.gene20311:CDS 30.0%
! TATGTATCAGCCTTCTAAAA+GGG - chr5.1:7827760-7827779 MS.gene20311:CDS 30.0%
! TCTGTTTTGTCTTCTTTACT+TGG - chr5.1:7828337-7828356 MS.gene20311:CDS 30.0%
!! AACCTATAAAGTGTTGTGTT+TGG + chr5.1:7828121-7828140 None:intergenic 30.0%
!! GCAGATTGTTGTTTCAATAT+TGG + chr5.1:7829009-7829028 None:intergenic 30.0%
AACCACTTTGAATCACAAGA+AGG + chr5.1:7828981-7829000 None:intergenic 35.0%
AAGGATAAGAGACATAGCAA+TGG - chr5.1:7828610-7828629 MS.gene20311:CDS 35.0%
ACCATCAAAGAAATCTTTGC+AGG + chr5.1:7828454-7828473 None:intergenic 35.0%
ACCTGCAAAGATTTCTTTGA+TGG - chr5.1:7828450-7828469 MS.gene20311:CDS 35.0%
AGAGGAACAACATTCTCAAA+TGG - chr5.1:7828032-7828051 MS.gene20311:CDS 35.0%
AGTTGAATTGTTGTCTGGTA+TGG - chr5.1:7828723-7828742 MS.gene20311:CDS 35.0%
ATCAAAAGTCTCACCATGAT+AGG + chr5.1:7828844-7828863 None:intergenic 35.0%
CTAAAAGGGATATGGTTGTT+GGG - chr5.1:7827774-7827793 MS.gene20311:CDS 35.0%
CTATGTATCAGCCTTCTAAA+AGG - chr5.1:7827759-7827778 MS.gene20311:CDS 35.0%
CTCCAAACACAACACTTTAT+AGG - chr5.1:7828116-7828135 MS.gene20311:CDS 35.0%
CTTACACTTGGAAATGAAGA+TGG + chr5.1:7829070-7829089 None:intergenic 35.0%
TACAACTATGCAAGAGCTTT+GGG - chr5.1:7828081-7828100 MS.gene20311:CDS 35.0%
TATCTCCAGCCATAGTTTAT+TGG - chr5.1:7828567-7828586 MS.gene20311:CDS 35.0%
TCTAAAAGGGATATGGTTGT+TGG - chr5.1:7827773-7827792 MS.gene20311:CDS 35.0%
TGTTAACACCATACCTATCA+TGG - chr5.1:7828828-7828847 MS.gene20311:CDS 35.0%
! ACAACCATATCCCTTTTAGA+AGG + chr5.1:7827773-7827792 None:intergenic 35.0%
! AGTACTTGTTGATGAAGAAG+AGG - chr5.1:7828164-7828183 MS.gene20311:CDS 35.0%
! GATGGTTGATATCTTGAATG+AGG - chr5.1:7828468-7828487 MS.gene20311:CDS 35.0%
! GTTTATTGGTTGGATTGATG+AGG - chr5.1:7828581-7828600 MS.gene20311:CDS 35.0%
!! CTTTTAGAAGGCTGATACAT+AGG + chr5.1:7827761-7827780 None:intergenic 35.0%
!! TGCATTGGTTCAAGAGAAAT+TGG + chr5.1:7827977-7827996 None:intergenic 35.0%
AAGAGGTCTGAAGATGTTCA+AGG - chr5.1:7828181-7828200 MS.gene20311:CDS 40.0%
AAGTCTCACCATGATAGGTA+TGG + chr5.1:7828839-7828858 None:intergenic 40.0%
AATCACAAGAAGGAGCTTAG+CGG + chr5.1:7828971-7828990 None:intergenic 40.0%
ACTATGGCTGGAGATAATCT+CGG + chr5.1:7828563-7828582 None:intergenic 40.0%
AGACTAAAATCAGTTGCACG+CGG - chr5.1:7828500-7828519 MS.gene20311:CDS 40.0%
AGCTAGAGTTCATGCTTTGA+AGG - chr5.1:7828249-7828268 MS.gene20311:CDS 40.0%
CAAAGCTCTTGCATAGTTGT+AGG + chr5.1:7828082-7828101 None:intergenic 40.0%
CAGAACAGTGTGCTTCTATA+AGG - chr5.1:7828657-7828676 MS.gene20311:CDS 40.0%
CCAACAGCATGTGAAGTAAA+AGG + chr5.1:7828364-7828383 None:intergenic 40.0%
CTACAACTATGCAAGAGCTT+TGG - chr5.1:7828080-7828099 MS.gene20311:CDS 40.0%
CTCCTTCTTGTGATTCAAAG+TGG - chr5.1:7828976-7828995 MS.gene20311:CDS 40.0%
GAGAATGTTGTTCCTCTCAT+AGG + chr5.1:7828029-7828048 None:intergenic 40.0%
GAGACACTCAAGAAGGTTAA+GGG - chr5.1:7828223-7828242 MS.gene20311:CDS 40.0%
GATATGGTTGTTGGGTATGA+GGG - chr5.1:7827782-7827801 MS.gene20311:CDS 40.0%
GGGCTAATTCCAAACAATCA+TGG + chr5.1:7828750-7828769 None:intergenic 40.0%
GTTGATTGTGGTGGTATTGT+AGG - chr5.1:7827866-7827885 MS.gene20311:CDS 40.0%
TCCAACCAATAAACTATGGC+TGG + chr5.1:7828575-7828594 None:intergenic 40.0%
TCCAGCCATAGTTTATTGGT+TGG - chr5.1:7828571-7828590 MS.gene20311:CDS 40.0%
TCGTTATCGTCAATACCTGA+AGG - chr5.1:7828784-7828803 MS.gene20311:CDS 40.0%
TGATGGTCAGATTCTAGATC+TGG - chr5.1:7827811-7827830 MS.gene20311:CDS 40.0%
TGGTATGGACCATGATTGTT+TGG - chr5.1:7828738-7828757 MS.gene20311:CDS 40.0%
! AAGTGTTGTGTTTGGAGTGA+TGG + chr5.1:7828113-7828132 None:intergenic 40.0%
! CCTTTTACTTCACATGCTGT+TGG - chr5.1:7828361-7828380 MS.gene20311:CDS 40.0%
! GGAATTAGCCCTTTGTGTTT+TGG - chr5.1:7828759-7828778 MS.gene20311:CDS 40.0%
! TATAGAAGCACACTGTTCTG+AGG + chr5.1:7828657-7828676 None:intergenic 40.0%
! TCAGCCTTCTAAAAGGGATA+TGG - chr5.1:7827766-7827785 MS.gene20311:CDS 40.0%
! TCATGGTGAGACTTTTGATG+AGG - chr5.1:7828845-7828864 MS.gene20311:CDS 40.0%
!! ATTGTAGGAACTGGTGTTGT+TGG - chr5.1:7827881-7827900 MS.gene20311:CDS 40.0%
!! GCTGTTGGTTCTGAAGTTAT+TGG - chr5.1:7828376-7828395 MS.gene20311:CDS 40.0%
!! TGGATTGATGAGGCTTGTTA+AGG - chr5.1:7828591-7828610 MS.gene20311:CDS 40.0%
AAGTCTGTGATTGATGGAGG+TGG - chr5.1:7828310-7828329 MS.gene20311:CDS 45.0%
AGAGTAACAGGGTCTTGCAT+TGG + chr5.1:7827992-7828011 None:intergenic 45.0%
ATCGTCAATACCTGAAGGCA+GGG - chr5.1:7828789-7828808 MS.gene20311:CDS 45.0%
CATAGCAATGGAATCTGTCC+TGG - chr5.1:7828622-7828641 MS.gene20311:CDS 45.0%
CGTAAGTCTGTGATTGATGG+AGG - chr5.1:7828307-7828326 MS.gene20311:CDS 45.0%
GCACGTAAGTCTGTGATTGA+TGG - chr5.1:7828304-7828323 MS.gene20311:CDS 45.0%
GGAGACACTCAAGAAGGTTA+AGG - chr5.1:7828222-7828241 MS.gene20311:CDS 45.0%
GGATATGGTTGTTGGGTATG+AGG - chr5.1:7827781-7827800 MS.gene20311:CDS 45.0%
GTCCAGTGCAAAGAGTAACA+GGG + chr5.1:7828003-7828022 None:intergenic 45.0%
TATCGTCAATACCTGAAGGC+AGG - chr5.1:7828788-7828807 MS.gene20311:CDS 45.0%
TGGACACAGCTGTGAAAGAT+GGG - chr5.1:7827831-7827850 MS.gene20311:CDS 45.0%
TGTCCAGTGCAAAGAGTAAC+AGG + chr5.1:7828004-7828023 None:intergenic 45.0%
TGTGGTGGTATTGTAGGAAC+TGG - chr5.1:7827872-7827891 MS.gene20311:CDS 45.0%
! AGCTGTGAAAGATGGGGTTT+TGG - chr5.1:7827838-7827857 MS.gene20311:CDS 45.0%
! GAAGGAGCTTCATCAAGTTG+TGG - chr5.1:7828267-7828286 MS.gene20311:CDS 45.0%
! GCTGTGAAAGATGGGGTTTT+GGG - chr5.1:7827839-7827858 MS.gene20311:CDS 45.0%
! GGTTCTGGTAAGCTTACACT+TGG + chr5.1:7829082-7829101 None:intergenic 45.0%
!!! GTTTTGGGTGGTGTTGATTG+TGG - chr5.1:7827854-7827873 MS.gene20311:CDS 45.0%
AGGAAGAGCTTCTGAGCTTG+TGG - chr5.1:7828201-7828220 MS.gene20311:CDS 50.0%
ATGGTTGTTGGGTATGAGGG+TGG - chr5.1:7827785-7827804 MS.gene20311:CDS 50.0%
CTGGACACAGCTGTGAAAGA+TGG - chr5.1:7827830-7827849 MS.gene20311:CDS 50.0%
GACCCTGTTACTCTTTGCAC+TGG - chr5.1:7827998-7828017 MS.gene20311:CDS 50.0%
GCACTGGACAAACCTATGAG+AGG - chr5.1:7828014-7828033 MS.gene20311:CDS 50.0%
GCTTGTGGAGACACTCAAGA+AGG - chr5.1:7828216-7828235 MS.gene20311:CDS 50.0%
GGACACAGCTGTGAAAGATG+GGG - chr5.1:7827832-7827851 MS.gene20311:CDS 50.0%
TTCAAAGCTACCCTGCCTTC+AGG + chr5.1:7828802-7828821 None:intergenic 50.0%
!! AGAAGCACACTGTTCTGAGG+AGG + chr5.1:7828654-7828673 None:intergenic 50.0%
!! GTTCTGAGGAGGCTTAATCC+AGG + chr5.1:7828643-7828662 None:intergenic 50.0%
!! TGAAGGCAGGGTAGCTTTGA+AGG - chr5.1:7828801-7828820 MS.gene20311:CDS 50.0%
!! TTGGGTGGTGTTGATTGTGG+TGG - chr5.1:7827857-7827876 MS.gene20311:CDS 50.0%
!!! GTGAAAGATGGGGTTTTGGG+TGG - chr5.1:7827842-7827861 MS.gene20311:CDS 50.0%
GCAATCGACGAACACTCCTC+GGG + chr5.1:7828931-7828950 None:intergenic 55.0%
GGCAATCGACGAACACTCCT+CGG + chr5.1:7828932-7828951 None:intergenic 55.0%
GTTGTTGGGTATGAGGGTGG+TGG - chr5.1:7827788-7827807 MS.gene20311:CDS 55.0%
! CGTCGATTGCCGTTGATGCA+GGG - chr5.1:7828941-7828960 MS.gene20311:CDS 55.0%
! TCGTCGATTGCCGTTGATGC+AGG - chr5.1:7828940-7828959 MS.gene20311:CDS 55.0%
AGCGGCAAGCCCTGCATCAA+CGG + chr5.1:7828953-7828972 None:intergenic 60.0%
TGTTTGCAAGCTCGCACCCG+AGG - chr5.1:7828912-7828931 MS.gene20311:CDS 60.0%
! GGGTATGAGGGTGGTGGTGA+TGG - chr5.1:7827794-7827813 MS.gene20311:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 7827755 7829110 7827755 ID=MS.gene20311
chr5.1 mRNA 7827755 7829110 7827755 ID=MS.gene20311.t1;Parent=MS.gene20311
chr5.1 exon 7827755 7829110 7827755 ID=MS.gene20311.t1.exon1;Parent=MS.gene20311.t1
chr5.1 CDS 7827755 7829110 7827755 ID=cds.MS.gene20311.t1;Parent=MS.gene20311.t1
Gene Sequence

>MS.gene20311

ATGCCTATGTATCAGCCTTCTAAAAGGGATATGGTTGTTGGGTATGAGGGTGGTGGTGATGGTCAGATTCTAGATCTGGACACAGCTGTGAAAGATGGGGTTTTGGGTGGTGTTGATTGTGGTGGTATTGTAGGAACTGGTGTTGTTGGTGAGAAATTAGATCTGAGAAAAATGATTCAAGAGCTTGAGTTATGTGAAGTACCAAGTGTTTTCATTTGTCCAATTTCTCTTGAACCAATGCAAGACCCTGTTACTCTTTGCACTGGACAAACCTATGAGAGGAACAACATTCTCAAATGGTTTAACATGGGACACTTTACTTGTCCTACAACTATGCAAGAGCTTTGGGATGATTCCATCACTCCAAACACAACACTTTATAGGTTAATATATACTTGGTTTTCTCAGAAGTACTTGTTGATGAAGAAGAGGTCTGAAGATGTTCAAGGAAGAGCTTCTGAGCTTGTGGAGACACTCAAGAAGGTTAAGGGTCAAGCTAGAGTTCATGCTTTGAAGGAGCTTCATCAAGTTGTGGCTGTTCATGCTACTGCACGTAAGTCTGTGATTGATGGAGGTGGTGTTTCTGTTTTGTCTTCTTTACTTGGTCCTTTTACTTCACATGCTGTTGGTTCTGAAGTTATTGGTATTCTTGTTAGTTTGACACTTGATTCTGAATCTAAAAAGAATCTTATGCAACCTGCAAAGATTTCTTTGATGGTTGATATCTTGAATGAGGGTTCAATTGAGACTAAAATCAGTTGCACGCGGTTGATTGAAACGTTAATTGAGGAGAAAAATTTTCGTTCCGAGATTATCTCCAGCCATAGTTTATTGGTTGGATTGATGAGGCTTGTTAAGGATAAGAGACATAGCAATGGAATCTGTCCTGGATTAAGCCTCCTCAGAACAGTGTGCTTCTATAAGGAAGTTAAGATTTTGTTAGTTAGCATTGGTGCTGTTTCTCAATTAGTTGAATTGTTGTCTGGTATGGACCATGATTGTTTGGAATTAGCCCTTTGTGTTTTGGATTCGTTATCGTCAATACCTGAAGGCAGGGTAGCTTTGAAGGAATGTGTTAACACCATACCTATCATGGTGAGACTTTTGATGAGGATATCAGAAAGCTGTACTCAGTATGCATTGTCAATTTTGTGGTCTGTTTGCAAGCTCGCACCCGAGGAGTGTTCGTCGATTGCCGTTGATGCAGGGCTTGCCGCTAAGCTCCTTCTTGTGATTCAAAGTGGTTGCAATCCAATATTGAAACAACAATCTGCTGAGCTTTTGAAACTATGTAGTTTGAATTATTCAGATACCATCTTCATTTCCAAGTGTAAGCTTACCAGAACCATCCAATGA
Protein sequence

>MS.gene20311.t1

MPMYQPSKRDMVVGYEGGGDGQILDLDTAVKDGVLGGVDCGGIVGTGVVGEKLDLRKMIQELELCEVPSVFICPISLEPMQDPVTLCTGQTYERNNILKWFNMGHFTCPTTMQELWDDSITPNTTLYRLIYTWFSQKYLLMKKRSEDVQGRASELVETLKKVKGQARVHALKELHQVVAVHATARKSVIDGGGVSVLSSLLGPFTSHAVGSEVIGILVSLTLDSESKKNLMQPAKISLMVDILNEGSIETKISCTRLIETLIEEKNFRSEIISSHSLLVGLMRLVKDKRHSNGICPGLSLLRTVCFYKEVKILLVSIGAVSQLVELLSGMDHDCLELALCVLDSLSSIPEGRVALKECVNTIPIMVRLLMRISESCTQYALSILWSVCKLAPEECSSIAVDAGLAAKLLLVIQSGCNPILKQQSAELLKLCSLNYSDTIFISKCKLTRTIQ