Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20325.t1 | XP_013469267.1 | 94.3 | 209 | 12 | 0 | 1 | 209 | 1 | 209 | 2.10E-97 | 365.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20325.t1 | A0A072VZ22 | 94.3 | 209 | 12 | 0 | 1 | 209 | 1 | 209 | 1.5e-97 | 365.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053074 | MS.gene20325 | 0.833929 | 4.02E-56 | -1.69E-46 |
| MS.gene054596 | MS.gene20325 | 0.810547 | 1.09E-50 | -1.69E-46 |
| MS.gene059006 | MS.gene20325 | 0.806603 | 7.59E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20325.t1 | MTR_1g090680 | 96.172 | 209 | 8 | 0 | 1 | 209 | 1 | 209 | 5.52e-129 | 396 |
| MS.gene20325.t1 | MTR_1g090673 | 84.135 | 208 | 32 | 1 | 3 | 209 | 2 | 209 | 4.92e-109 | 341 |
| MS.gene20325.t1 | MTR_1g090690 | 64.356 | 202 | 68 | 3 | 10 | 209 | 15 | 214 | 1.46e-74 | 244 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 52 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTGAAGAGTATGCGGTTT+TGG | 0.209747 | 1.3:+63410636 | MS.gene20325:CDS |
| AATCTCAATCACCTTATTAC+TGG | 0.315640 | 1.3:-63411085 | None:intergenic |
| AAATTTAATAACCAGTAATA+AGG | 0.404155 | 1.3:+63411074 | MS.gene20325:CDS |
| TACTTCTACGATGGTGGAAC+AGG | 0.418439 | 1.3:-63411016 | None:intergenic |
| TGGAGAAGCTTGATCAAATT+CGG | 0.423345 | 1.3:+63411043 | MS.gene20325:CDS |
| ATCAGTGGTTGCAAAATTCC+TGG | 0.432163 | 1.3:+63410615 | MS.gene20325:CDS |
| GCAGCGCGGGGATGGAGAAC+AGG | 0.438660 | 1.3:+63410732 | MS.gene20325:CDS |
| ATCAGTGAAGAGGAGGAGGT+GGG | 0.452432 | 1.3:+63410837 | MS.gene20325:CDS |
| GAAAATAATGAATTTAAACT+TGG | 0.466373 | 1.3:+63410939 | MS.gene20325:CDS |
| CAGGTTGAGCAGCGCGGGGA+TGG | 0.466619 | 1.3:+63410724 | MS.gene20325:CDS |
| AATCAGTGAAGAGGAGGAGG+TGG | 0.476471 | 1.3:+63410836 | MS.gene20325:CDS |
| TAGAACAGGTTGAGCAGCGC+GGG | 0.477218 | 1.3:+63410719 | MS.gene20325:CDS |
| GTTGAAACTCCCGTTGTAAG+AGG | 0.479721 | 1.3:+63410976 | MS.gene20325:CDS |
| ATACAGCGAGGCACGTAGAA+TGG | 0.482831 | 1.3:+63410662 | MS.gene20325:CDS |
| GGTGCTTCTCCTCTTACAAC+GGG | 0.485948 | 1.3:-63410985 | None:intergenic |
| TCAGTGAAGAGGAGGAGGTG+GGG | 0.485983 | 1.3:+63410838 | MS.gene20325:CDS |
| GAAAGAGCTGAGGTCAATGC+GGG | 0.505992 | 1.3:+63410690 | MS.gene20325:CDS |
| CGGTGCTTCTCCTCTTACAA+CGG | 0.507363 | 1.3:-63410986 | None:intergenic |
| TAATAAGGTGATTGAGATTG+TGG | 0.511886 | 1.3:+63411089 | MS.gene20325:CDS |
| AATGCGGGGATTGTTAGAAC+AGG | 0.523410 | 1.3:+63410705 | MS.gene20325:CDS |
| CACCATCGTAGAAGTAGCTA+TGG | 0.525095 | 1.3:+63411023 | MS.gene20325:CDS |
| TAAAATCAGTGAAGAGGAGG+AGG | 0.533266 | 1.3:+63410833 | MS.gene20325:CDS |
| AGAAGAGTTCACATGGCTGC+TGG | 0.542293 | 1.3:+63410571 | None:intergenic |
| AGGAACAAGAGTTGAAAGAA+TGG | 0.543642 | 1.3:+63410752 | MS.gene20325:CDS |
| ATACAAGGTTGGTAAAGAGC+TGG | 0.545349 | 1.3:+63410882 | MS.gene20325:CDS |
| AGAAACTGTGTGGTTGGAAG+AGG | 0.550252 | 1.3:+63411179 | MS.gene20325:CDS |
| TGAAAGAGAAACTGTGTGGT+TGG | 0.552834 | 1.3:+63411173 | MS.gene20325:CDS |
| CTCCATAGCTACTTCTACGA+TGG | 0.553727 | 1.3:-63411025 | None:intergenic |
| GCATTGGTATGACAAATACA+AGG | 0.562770 | 1.3:+63410867 | MS.gene20325:CDS |
| GGAACAAGAGTTGAAAGAAT+GGG | 0.566887 | 1.3:+63410753 | MS.gene20325:CDS |
| CATAGCTACTTCTACGATGG+TGG | 0.579160 | 1.3:-63411022 | None:intergenic |
| AGCTGGAGGAGATCAGAAAG+AGG | 0.581622 | 1.3:+63410899 | MS.gene20325:CDS |
| AGAATGGGCGGAGAAGTTGA+AGG | 0.587022 | 1.3:+63410768 | MS.gene20325:CDS |
| TTGTGGAAGAAGTAAAAGAC+CGG | 0.596672 | 1.3:+63411106 | MS.gene20325:CDS |
| TCGTGATAAAATCAGTGAAG+AGG | 0.605485 | 1.3:+63410827 | MS.gene20325:CDS |
| ATTCCTGGTTGAAGAGTATG+CGG | 0.609971 | 1.3:+63410630 | MS.gene20325:CDS |
| TGATAAAATCAGTGAAGAGG+AGG | 0.614245 | 1.3:+63410830 | MS.gene20325:CDS |
| CGTTGTAAGAGGAGAAGCAC+CGG | 0.615629 | 1.3:+63410987 | MS.gene20325:CDS |
| TGGTATGACAAATACAAGGT+TGG | 0.619950 | 1.3:+63410871 | MS.gene20325:CDS |
| CAAGGTTGGTAAAGAGCTGG+AGG | 0.637417 | 1.3:+63410885 | MS.gene20325:CDS |
| ATGGTGGAACAGGAAGGTGC+CGG | 0.647720 | 1.3:-63411006 | None:intergenic |
| TGGCGTTGCAGTCGATGTTG+TGG | 0.648979 | 1.3:+63410591 | MS.gene20325:CDS |
| TCTACGATGGTGGAACAGGA+AGG | 0.656682 | 1.3:-63411012 | None:intergenic |
| AAACCGCATACTCTTCAACC+AGG | 0.662234 | 1.3:-63410633 | None:intergenic |
| AGAAAGAGCTGAGGTCAATG+CGG | 0.667050 | 1.3:+63410689 | MS.gene20325:CDS |
| AGAACAGGTTGAGCAGCGCG+GGG | 0.671879 | 1.3:+63410720 | MS.gene20325:CDS |
| AGTCGATGTTGTGGTATCAG+TGG | 0.681138 | 1.3:+63410600 | MS.gene20325:CDS |
| ACAAGAGTTGAAAGAATGGG+CGG | 0.683061 | 1.3:+63410756 | MS.gene20325:CDS |
| AATGGATTGAGAAAGAGCTG+AGG | 0.698893 | 1.3:+63410680 | MS.gene20325:CDS |
| TTAGAACAGGTTGAGCAGCG+CGG | 0.707331 | 1.3:+63410718 | MS.gene20325:CDS |
| AAAGAGCTGAGGTCAATGCG+GGG | 0.721739 | 1.3:+63410691 | MS.gene20325:CDS |
| CAAATGAAAGAGAAACTGTG+TGG | 0.768788 | 1.3:+63411169 | MS.gene20325:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTTAATAACCAGTAATA+AGG | + | chr1.3:63411074-63411093 | MS.gene20325:CDS | 15.0% |
| !! | GAAAATAATGAATTTAAACT+TGG | + | chr1.3:63410939-63410958 | MS.gene20325:CDS | 15.0% |
| ! | CATATCCTTCATAAAGATAA+AGG | - | chr1.3:63411296-63411315 | None:intergenic | 25.0% |
| ! | GAAAAATGGTTCAAAATGTA+TGG | + | chr1.3:63411258-63411277 | MS.gene20325:intron | 25.0% |
| !!! | ATTTAGACACAATTTTTTGC+AGG | - | chr1.3:63411140-63411159 | None:intergenic | 25.0% |
| AAGGTCCTTTATCTTTATGA+AGG | + | chr1.3:63411288-63411307 | MS.gene20325:intron | 30.0% | |
| AATCTCAATCACCTTATTAC+TGG | - | chr1.3:63411088-63411107 | None:intergenic | 30.0% | |
| AATGGTTCAAAATGTATGGT+TGG | + | chr1.3:63411262-63411281 | MS.gene20325:intron | 30.0% | |
| AATTACTCGGAAAAAGTTGT+TGG | + | chr1.3:63411216-63411235 | MS.gene20325:intron | 30.0% | |
| GAACGAAGTTTGTAATTACT+CGG | + | chr1.3:63411203-63411222 | MS.gene20325:intron | 30.0% | |
| TAATAAGGTGATTGAGATTG+TGG | + | chr1.3:63411089-63411108 | MS.gene20325:CDS | 30.0% | |
| TCATTGCAAGAAGAGAAAAA+TGG | + | chr1.3:63411244-63411263 | MS.gene20325:intron | 30.0% | |
| AGGAACAAGAGTTGAAAGAA+TGG | + | chr1.3:63410752-63410771 | MS.gene20325:CDS | 35.0% | |
| CAAAATGTATGGTTGGAAGA+AGG | + | chr1.3:63411269-63411288 | MS.gene20325:intron | 35.0% | |
| CAAATGAAAGAGAAACTGTG+TGG | + | chr1.3:63411169-63411188 | MS.gene20325:CDS | 35.0% | |
| GGAACAAGAGTTGAAAGAAT+GGG | + | chr1.3:63410753-63410772 | MS.gene20325:CDS | 35.0% | |
| TCGTGATAAAATCAGTGAAG+AGG | + | chr1.3:63410827-63410846 | MS.gene20325:CDS | 35.0% | |
| TGATAAAATCAGTGAAGAGG+AGG | + | chr1.3:63410830-63410849 | MS.gene20325:CDS | 35.0% | |
| TGGAGAAGCTTGATCAAATT+CGG | + | chr1.3:63411043-63411062 | MS.gene20325:CDS | 35.0% | |
| TGGTATGACAAATACAAGGT+TGG | + | chr1.3:63410871-63410890 | MS.gene20325:CDS | 35.0% | |
| TTGTGGAAGAAGTAAAAGAC+CGG | + | chr1.3:63411106-63411125 | MS.gene20325:CDS | 35.0% | |
| ! | GCATTGGTATGACAAATACA+AGG | + | chr1.3:63410867-63410886 | MS.gene20325:CDS | 35.0% |
| ACAAGAGTTGAAAGAATGGG+CGG | + | chr1.3:63410756-63410775 | MS.gene20325:CDS | 40.0% | |
| ATCAGTGGTTGCAAAATTCC+TGG | + | chr1.3:63410615-63410634 | MS.gene20325:CDS | 40.0% | |
| TAAAATCAGTGAAGAGGAGG+AGG | + | chr1.3:63410833-63410852 | MS.gene20325:CDS | 40.0% | |
| TGAAAGAGAAACTGTGTGGT+TGG | + | chr1.3:63411173-63411192 | MS.gene20325:CDS | 40.0% | |
| ! | ATTCCTGGTTGAAGAGTATG+CGG | + | chr1.3:63410630-63410649 | MS.gene20325:CDS | 40.0% |
| !! | AATGGATTGAGAAAGAGCTG+AGG | + | chr1.3:63410680-63410699 | MS.gene20325:CDS | 40.0% |
| !! | ATACAAGGTTGGTAAAGAGC+TGG | + | chr1.3:63410882-63410901 | MS.gene20325:CDS | 40.0% |
| !! | GTTGAAGAGTATGCGGTTTT+GGG | + | chr1.3:63410637-63410656 | MS.gene20325:CDS | 40.0% |
| !! | TTGAAGAGTATGCGGTTTTG+GGG | + | chr1.3:63410638-63410657 | MS.gene20325:CDS | 40.0% |
| !!! | TTTTTTGCAGGTCTTTGAGC+CGG | - | chr1.3:63411128-63411147 | None:intergenic | 40.0% |
| AAACCGCATACTCTTCAACC+AGG | - | chr1.3:63410636-63410655 | None:intergenic | 45.0% | |
| AATGCGGGGATTGTTAGAAC+AGG | + | chr1.3:63410705-63410724 | MS.gene20325:CDS | 45.0% | |
| AGAAACTGTGTGGTTGGAAG+AGG | + | chr1.3:63411179-63411198 | MS.gene20325:CDS | 45.0% | |
| AGAAAGAGCTGAGGTCAATG+CGG | + | chr1.3:63410689-63410708 | MS.gene20325:CDS | 45.0% | |
| AGTCGATGTTGTGGTATCAG+TGG | + | chr1.3:63410600-63410619 | MS.gene20325:CDS | 45.0% | |
| CACCATCGTAGAAGTAGCTA+TGG | + | chr1.3:63411023-63411042 | MS.gene20325:CDS | 45.0% | |
| CATAGCTACTTCTACGATGG+TGG | - | chr1.3:63411025-63411044 | None:intergenic | 45.0% | |
| CTCCATAGCTACTTCTACGA+TGG | - | chr1.3:63411028-63411047 | None:intergenic | 45.0% | |
| GTTGAAACTCCCGTTGTAAG+AGG | + | chr1.3:63410976-63410995 | MS.gene20325:CDS | 45.0% | |
| !! | GGTTGAAGAGTATGCGGTTT+TGG | + | chr1.3:63410636-63410655 | MS.gene20325:CDS | 45.0% |
| !! | TACTTCTACGATGGTGGAAC+AGG | - | chr1.3:63411019-63411038 | None:intergenic | 45.0% |
| !! | TGAAGAGTATGCGGTTTTGG+GGG | + | chr1.3:63410639-63410658 | MS.gene20325:CDS | 45.0% |
| AAAGAGCTGAGGTCAATGCG+GGG | + | chr1.3:63410691-63410710 | MS.gene20325:CDS | 50.0% | |
| AATCAGTGAAGAGGAGGAGG+TGG | + | chr1.3:63410836-63410855 | MS.gene20325:CDS | 50.0% | |
| AGAATGGGCGGAGAAGTTGA+AGG | + | chr1.3:63410768-63410787 | MS.gene20325:CDS | 50.0% | |
| AGCTGGAGGAGATCAGAAAG+AGG | + | chr1.3:63410899-63410918 | MS.gene20325:CDS | 50.0% | |
| ATACAGCGAGGCACGTAGAA+TGG | + | chr1.3:63410662-63410681 | MS.gene20325:CDS | 50.0% | |
| ATCAGTGAAGAGGAGGAGGT+GGG | + | chr1.3:63410837-63410856 | MS.gene20325:CDS | 50.0% | |
| CGGTGCTTCTCCTCTTACAA+CGG | - | chr1.3:63410989-63411008 | None:intergenic | 50.0% | |
| GAAAGAGCTGAGGTCAATGC+GGG | + | chr1.3:63410690-63410709 | MS.gene20325:CDS | 50.0% | |
| GGTGCTTCTCCTCTTACAAC+GGG | - | chr1.3:63410988-63411007 | None:intergenic | 50.0% | |
| ! | CGTTGTAAGAGGAGAAGCAC+CGG | + | chr1.3:63410987-63411006 | MS.gene20325:CDS | 50.0% |
| ! | TTAGAACAGGTTGAGCAGCG+CGG | + | chr1.3:63410718-63410737 | MS.gene20325:CDS | 50.0% |
| !! | CAAGGTTGGTAAAGAGCTGG+AGG | + | chr1.3:63410885-63410904 | MS.gene20325:CDS | 50.0% |
| !! | TCTACGATGGTGGAACAGGA+AGG | - | chr1.3:63411015-63411034 | None:intergenic | 50.0% |
| TCAGTGAAGAGGAGGAGGTG+GGG | + | chr1.3:63410838-63410857 | MS.gene20325:CDS | 55.0% | |
| TGGCGTTGCAGTCGATGTTG+TGG | + | chr1.3:63410591-63410610 | MS.gene20325:CDS | 55.0% | |
| ! | ATGGTGGAACAGGAAGGTGC+CGG | - | chr1.3:63411009-63411028 | None:intergenic | 55.0% |
| ! | GGAGGTGGGGAGTTTTGCAT+TGG | + | chr1.3:63410851-63410870 | MS.gene20325:CDS | 55.0% |
| ! | TAGAACAGGTTGAGCAGCGC+GGG | + | chr1.3:63410719-63410738 | MS.gene20325:CDS | 55.0% |
| ! | AGAACAGGTTGAGCAGCGCG+GGG | + | chr1.3:63410720-63410739 | MS.gene20325:CDS | 60.0% |
| !!! | CGGTTTTGGGGGATACAGCG+AGG | + | chr1.3:63410650-63410669 | MS.gene20325:CDS | 60.0% |
| GCAGCGCGGGGATGGAGAAC+AGG | + | chr1.3:63410732-63410751 | MS.gene20325:CDS | 70.0% | |
| ! | CAGGTTGAGCAGCGCGGGGA+TGG | + | chr1.3:63410724-63410743 | MS.gene20325:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 63410583 | 63411321 | 63410583 | ID=MS.gene20325 |
| chr1.3 | mRNA | 63410583 | 63411321 | 63410583 | ID=MS.gene20325.t1;Parent=MS.gene20325 |
| chr1.3 | exon | 63410583 | 63411209 | 63410583 | ID=MS.gene20325.t1.exon1;Parent=MS.gene20325.t1 |
| chr1.3 | CDS | 63410583 | 63411209 | 63410583 | ID=cds.MS.gene20325.t1;Parent=MS.gene20325.t1 |
| chr1.3 | exon | 63411310 | 63411321 | 63411310 | ID=MS.gene20325.t1.exon2;Parent=MS.gene20325.t1 |
| chr1.3 | CDS | 63411310 | 63411321 | 63411310 | ID=cds.MS.gene20325.t1;Parent=MS.gene20325.t1 |
| Gene Sequence |
| Protein sequence |
