AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20706

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20706.t1 MTR_1g088885 96.990 598 18 0 8 605 24 621 0.0 1186
MS.gene20706.t1 MTR_7g108290 77.375 579 121 6 8 579 24 599 0.0 907
MS.gene20706.t1 MTR_4g022330 47.619 567 271 9 19 575 33 583 3.19e-178 518
MS.gene20706.t1 MTR_7g073980 44.833 600 296 9 15 601 28 605 4.74e-169 496
MS.gene20706.t1 MTR_5g016060 41.760 625 293 16 19 605 39 630 9.05e-153 454
MS.gene20706.t1 MTR_4g095430 38.825 613 320 14 19 605 12 595 4.66e-135 407
MS.gene20706.t1 MTR_3g062540 36.469 606 306 14 19 605 23 568 2.37e-121 371
MS.gene20706.t1 MTR_5g016060 39.526 549 261 16 95 605 1 516 3.09e-117 358
MS.gene20706.t1 MTR_3g062540 36.239 585 294 14 40 605 2 526 1.79e-114 352
MS.gene20706.t1 MTR_4g093850 33.385 647 353 13 18 605 42 669 3.45e-113 353
MS.gene20706.t1 MTR_5g075640 34.461 621 308 14 19 605 24 579 1.20e-110 343
MS.gene20706.t1 MTR_2g009340 32.695 627 348 10 18 584 42 654 5.98e-109 342
MS.gene20706.t1 MTR_8g078900 32.855 627 329 9 1 556 1 606 4.79e-108 339
MS.gene20706.t1 MTR_8g078900 33.613 595 303 9 33 556 3 576 7.98e-106 333
MS.gene20706.t1 MTR_5g075640 34.333 600 295 14 40 605 2 536 6.57e-104 325
MS.gene20706.t1 MTR_2g084595 34.286 595 328 12 18 572 21 592 2.54e-103 326
MS.gene20706.t1 MTR_8g090220 33.777 601 294 14 3 556 39 582 4.71e-103 326
MS.gene20706.t1 MTR_8g078900 34.799 546 284 8 62 556 42 566 9.41e-102 322
MS.gene20706.t1 MTR_1g064360 35.055 542 302 10 18 537 24 537 4.86e-101 318
MS.gene20706.t1 MTR_1g094850 34.965 572 326 12 22 571 28 575 1.06e-100 319
MS.gene20706.t1 MTR_8g090220 35.938 512 255 11 3 479 39 512 6.48e-100 313
MS.gene20706.t1 MTR_2g018750 35.000 580 323 14 18 571 24 575 4.99e-99 314
MS.gene20706.t1 MTR_5g015790 36.820 478 266 9 18 478 25 483 3.57e-91 294
MS.gene20706.t1 MTR_1g106430 34.727 550 318 12 3 526 15 549 5.30e-91 294
MS.gene20706.t1 MTR_5g032780 37.476 515 264 13 18 487 24 525 3.63e-90 292
MS.gene20706.t1 MTR_1g014070 35.270 482 288 8 9 477 17 487 1.87e-88 287
MS.gene20706.t1 MTR_2g048390 35.973 442 240 8 18 428 24 453 4.22e-82 265
MS.gene20706.t1 MTR_4g093850 34.177 474 245 9 18 438 42 501 9.82e-82 266
MS.gene20706.t1 MTR_8g069330 36.025 483 270 10 23 487 29 490 2.76e-80 265
MS.gene20706.t1 MTR_4g134210 31.588 554 310 13 18 547 24 532 6.80e-77 256
MS.gene20706.t1 MTR_8g078900 32.533 458 251 7 18 428 18 464 5.94e-76 249
MS.gene20706.t1 MTR_8g078900 34.518 394 220 6 62 428 42 424 3.81e-70 233
MS.gene20706.t1 MTR_2g084595 33.634 443 245 8 18 428 21 446 9.54e-69 230
MS.gene20706.t1 MTR_1g082690 28.958 480 284 10 21 489 59 492 1.45e-66 227
MS.gene20706.t1 MTR_2g027410 30.290 482 273 10 17 476 23 463 7.35e-65 223
MS.gene20706.t1 MTR_4g011530 33.499 403 220 12 185 572 33 402 1.96e-61 211
MS.gene20706.t1 MTR_2g102730 33.867 375 197 9 194 556 24 359 3.21e-57 198
MS.gene20706.t1 MTR_2g027410 30.093 432 239 10 67 476 1 391 2.07e-54 193
MS.gene20706.t1 MTR_4g045767 26.289 582 354 14 23 574 7 543 4.41e-47 174
MS.gene20706.t1 MTR_4g074190 27.129 505 281 12 21 479 4 467 2.10e-42 160
MS.gene20706.t1 MTR_1g035720 25.210 595 316 19 23 561 6 527 1.65e-41 158
MS.gene20706.t1 MTR_1g105870 40.625 192 101 3 27 213 38 221 5.18e-41 150
MS.gene20706.t1 MTR_4g074190 27.093 454 248 10 68 479 9 421 9.65e-40 152
MS.gene20706.t1 MTR_4g074190 25.934 455 250 12 21 429 4 417 1.08e-31 128
MS.gene20706.t1 MTR_2g010890 25.986 431 264 8 86 499 1 393 5.40e-30 123
MS.gene20706.t1 MTR_2g048375 46.491 114 51 3 444 556 1 105 4.89e-22 94.0
MS.gene20706.t1 MTR_4g126990 89.189 37 4 0 1 37 1 37 1.48e-16 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20706.t1 AT1G03010 69.444 612 162 7 13 605 28 633 0.0 828
MS.gene20706.t1 AT1G03010 69.394 611 162 7 14 605 3 607 0.0 828
MS.gene20706.t1 AT1G03010 68.821 619 168 7 6 605 22 634 0.0 827
MS.gene20706.t1 AT2G47860 58.937 621 222 9 8 605 25 635 0.0 709
MS.gene20706.t1 AT2G47860 56.832 644 222 10 8 605 25 658 0.0 696
MS.gene20706.t1 AT2G47860 58.588 524 187 8 105 605 1 517 0.0 582
MS.gene20706.t1 AT2G47860 58.588 524 187 8 105 605 1 517 0.0 582
MS.gene20706.t1 AT2G47860 59.302 430 156 5 8 428 25 444 2.04e-175 505
MS.gene20706.t1 AT5G48800 45.648 563 278 7 19 575 40 580 8.90e-171 499
MS.gene20706.t1 AT2G47860 56.291 453 156 6 8 428 25 467 5.62e-170 492
MS.gene20706.t1 AT3G08570 43.471 582 295 10 7 575 22 582 5.80e-152 451
MS.gene20706.t1 AT3G08660 42.553 564 276 13 15 573 29 549 3.17e-142 425
MS.gene20706.t1 AT5G67385 39.706 612 313 12 19 605 24 604 1.75e-140 421
MS.gene20706.t1 AT3G08660 42.105 532 260 13 47 573 1 489 1.11e-129 391
MS.gene20706.t1 AT5G67385 39.153 590 303 12 41 605 1 559 2.56e-129 391
MS.gene20706.t1 AT5G67385 39.153 590 303 12 41 605 1 559 2.56e-129 391
MS.gene20706.t1 AT5G67385 39.153 590 303 12 41 605 1 559 2.56e-129 391
MS.gene20706.t1 AT5G67385 39.153 590 303 12 41 605 1 559 2.56e-129 391
MS.gene20706.t1 AT1G30440 36.026 619 315 12 18 592 24 605 5.59e-117 363
MS.gene20706.t1 AT2G30520 34.236 628 342 13 1 605 14 593 2.01e-111 346
MS.gene20706.t1 AT5G03250 36.082 582 306 15 22 572 28 574 2.37e-109 340
MS.gene20706.t1 AT5G13600 37.456 566 278 16 23 556 29 550 1.83e-103 325
MS.gene20706.t1 AT2G30520 34.465 589 318 12 40 605 2 545 2.64e-103 323
MS.gene20706.t1 AT2G30520 34.465 589 318 12 40 605 2 545 2.64e-103 323
MS.gene20706.t1 AT3G49970 34.114 598 280 15 33 605 7 515 3.27e-100 314
MS.gene20706.t1 AT3G49970 34.114 598 280 15 33 605 7 515 3.27e-100 314
MS.gene20706.t1 AT2G14820 34.815 540 320 11 18 539 25 550 3.36e-97 310
MS.gene20706.t1 AT2G14820 34.815 540 320 11 18 539 25 550 3.36e-97 310
MS.gene20706.t1 AT5G66560 32.909 629 299 12 22 556 21 620 8.81e-96 307
MS.gene20706.t1 AT3G44820 31.980 591 322 11 18 556 21 583 2.75e-94 303
MS.gene20706.t1 AT3G50840 33.040 569 310 9 8 556 4 521 9.00e-94 299
MS.gene20706.t1 AT3G08660 40.000 425 219 10 15 434 29 422 1.67e-93 294
MS.gene20706.t1 AT5G66560 32.848 618 292 12 33 556 3 591 1.68e-93 301
MS.gene20706.t1 AT5G66560 34.679 545 277 9 62 556 66 581 6.67e-92 296
MS.gene20706.t1 AT5G10250 32.522 575 292 15 18 553 48 565 2.38e-91 295
MS.gene20706.t1 AT1G67900 36.149 509 276 8 18 489 24 520 1.16e-88 288
MS.gene20706.t1 AT1G67900 36.149 509 276 8 18 489 24 520 1.16e-88 288
MS.gene20706.t1 AT1G67900 36.149 509 276 8 18 489 24 520 1.16e-88 288
MS.gene20706.t1 AT1G67900 36.149 509 276 8 18 489 24 520 1.16e-88 288
MS.gene20706.t1 AT1G67900 36.149 509 276 8 18 489 24 520 1.16e-88 288
MS.gene20706.t1 AT1G67900 36.149 509 276 8 18 489 24 520 1.16e-88 288
MS.gene20706.t1 AT5G10250 32.451 567 288 15 18 553 48 550 1.46e-88 287
MS.gene20706.t1 AT4G31820 35.818 483 266 10 9 478 17 468 1.32e-87 283
MS.gene20706.t1 AT3G50840 32.836 536 289 9 41 556 1 485 3.08e-85 276
MS.gene20706.t1 AT3G50840 32.836 536 289 9 41 556 1 485 3.08e-85 276
MS.gene20706.t1 AT5G67440 35.430 477 267 11 18 478 25 476 4.49e-82 269
MS.gene20706.t1 AT5G67440 35.430 477 267 11 18 478 25 476 4.49e-82 269
MS.gene20706.t1 AT4G37590 34.151 489 258 13 18 485 25 470 1.01e-80 265
MS.gene20706.t1 AT5G67440 35.498 462 257 11 33 478 3 439 2.34e-78 258
MS.gene20706.t1 AT3G26490 35.141 498 272 10 18 478 24 507 3.43e-78 259
MS.gene20706.t1 AT3G26490 35.141 498 272 10 18 478 24 507 3.43e-78 259
MS.gene20706.t1 AT3G26490 35.141 498 272 10 18 478 24 507 3.43e-78 259
MS.gene20706.t1 AT5G03250 33.602 497 266 14 105 572 1 462 4.01e-78 256
MS.gene20706.t1 AT2G23050 34.255 470 248 11 29 485 36 457 9.78e-78 254
MS.gene20706.t1 AT5G47800 32.520 492 294 9 17 493 23 491 3.86e-74 247
MS.gene20706.t1 AT5G47800 32.520 492 294 9 17 493 23 491 3.86e-74 247
MS.gene20706.t1 AT5G47800 32.520 492 294 9 17 493 23 491 3.86e-74 247
MS.gene20706.t1 AT4G31820 34.607 445 248 9 47 478 1 415 1.55e-73 244
MS.gene20706.t1 AT5G47800 32.394 497 288 9 17 493 136 604 4.23e-73 248
MS.gene20706.t1 AT5G66560 32.225 481 231 10 22 428 21 480 3.62e-64 219
MS.gene20706.t1 AT1G52770 33.158 380 207 5 194 556 28 377 1.07e-59 206
MS.gene20706.t1 AT3G26490 33.260 457 246 11 18 433 24 462 6.45e-59 205
MS.gene20706.t1 AT5G64330 35.262 363 204 9 249 590 371 723 6.40e-56 202
MS.gene20706.t1 AT5G64330 34.137 249 128 2 18 235 50 293 2.63e-43 166
MS.gene20706.t1 AT2G23050 32.391 389 207 10 105 485 1 341 1.83e-52 184
MS.gene20706.t1 AT3G19850 27.195 467 277 6 44 477 28 464 2.45e-52 189
MS.gene20706.t1 AT5G64330 41.870 246 126 4 249 483 312 551 1.36e-50 185
MS.gene20706.t1 AT5G64330 32.479 234 122 2 33 235 6 234 2.76e-37 147
MS.gene20706.t1 AT3G15570 35.548 301 147 5 200 479 35 309 2.14e-50 181
MS.gene20706.t1 AT5G64330 41.870 246 126 4 249 483 371 610 2.73e-50 185
MS.gene20706.t1 AT5G64330 34.137 249 128 2 18 235 50 293 1.59e-43 166
MS.gene20706.t1 AT5G48130 28.838 482 283 12 18 483 37 474 4.59e-50 184
MS.gene20706.t1 AT1G50280 27.216 485 298 10 16 472 3 460 6.96e-46 170
MS.gene20706.t1 AT1G50280 27.397 438 271 8 58 472 14 427 4.54e-44 164
MS.gene20706.t1 AT3G22104 28.540 459 267 10 22 463 6 420 1.42e-41 157
MS.gene20706.t1 AT3G03510 27.778 414 236 11 74 481 8 364 1.67e-37 145
MS.gene20706.t1 AT5G17580 25.996 477 302 11 22 483 22 462 1.31e-34 138
MS.gene20706.t1 AT5G17580 25.996 477 302 11 22 483 7 447 1.95e-34 138
MS.gene20706.t1 AT3G22104 27.390 387 223 7 91 463 3 345 3.06e-30 124
MS.gene20706.t1 AT3G22104 27.390 387 223 7 91 463 3 345 3.06e-30 124
MS.gene20706.t1 AT3G22104 27.390 387 223 7 91 463 3 345 3.06e-30 124
MS.gene20706.t1 AT3G49900 35.664 143 82 4 23 160 68 205 1.49e-11 67.4
MS.gene20706.t1 AT3G49900 35.664 143 82 4 23 160 68 205 1.50e-11 67.4

Find 94 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTGTCATTTCTAGAAAA+TGG 0.095087 1.2:-66540502 None:intergenic
CACCATTCTGAAGGTGTTTC+AGG 0.141106 1.2:-66540761 None:intergenic
CAGCTAGGTTTGTGTATTAC+AGG 0.213135 1.2:+66539978 MS.gene20706:CDS
ATTAAGCTTACTTAATTTCT+TGG 0.230580 1.2:-66542145 None:intergenic
CGCTCTGACTCCTTTATGTT+TGG 0.234991 1.2:-66541730 None:intergenic
AAGATAATTGTCAAGCAATT+TGG 0.245136 1.2:-66541338 None:intergenic
AACTTTGATGGAAACAAGTT+TGG 0.246026 1.2:-66541379 None:intergenic
TACAGTTTCCTCTAGTTTCT+AGG 0.257189 1.2:+66540309 MS.gene20706:intron
GTTTCGGATGAATGGTATTA+AGG 0.258143 1.2:+66542173 MS.gene20706:CDS
TTTCTAGGAGTGGAAGAATT+AGG 0.260242 1.2:+66540324 MS.gene20706:CDS
TACTTCTTGTAGATCTGATT+TGG 0.278731 1.2:+66541069 MS.gene20706:CDS
TTAAGAATTGAGCTTTGTTT+TGG 0.278917 1.2:-66541310 None:intergenic
AGGCTAATCTCTTCAGAAAT+TGG 0.287496 1.2:-66540635 None:intergenic
ATTGTGTCCTCCTCCGTTCA+TGG 0.295779 1.2:-66541887 None:intergenic
ACTGAATGACGCCTTCTCTT+TGG 0.299205 1.2:-66542231 None:intergenic
TGCAAAGAGCAACTTACTTC+TGG 0.313546 1.2:+66540695 MS.gene20706:CDS
GCAAGTGAAATGAACTTTGA+TGG 0.347009 1.2:-66541391 None:intergenic
GAGAATGAGACTCACTGATT+TGG 0.349387 1.2:+66542050 MS.gene20706:CDS
ATCAGTTGATATCTTCCTTA+AGG 0.362006 1.2:+66541462 MS.gene20706:CDS
TTCTGTCATTTCTAGAAAAT+GGG 0.365097 1.2:-66540501 None:intergenic
AGTGATCTTAGCATCGAAAT+AGG 0.383984 1.2:+66540019 MS.gene20706:CDS
CTTACAATTCGCGCATGATC+AGG 0.393723 1.2:-66541424 None:intergenic
AAAACAAAGCTCAATTCTTA+AGG 0.416708 1.2:+66541312 MS.gene20706:CDS
TTTCAGTTTCATCTCTCATT+CGG 0.419572 1.2:-66541258 None:intergenic
TTAATCTTGATTTCTTCAAA+AGG 0.420944 1.2:+66540804 MS.gene20706:CDS
TCAGGCAGTGGTGCAGGAAG+TGG 0.423415 1.2:+66541952 MS.gene20706:CDS
CTAGCTGCAAAATTCTGTTA+TGG 0.424115 1.2:+66540428 MS.gene20706:CDS
GTAAGCTTAATGCATTGTTT+CGG 0.426324 1.2:+66542157 MS.gene20706:CDS
GGTCAATTCCCTCAACGTTC+AGG 0.427741 1.2:+66541934 MS.gene20706:CDS
ACTTGTACTGCCATTTGAAC+TGG 0.430941 1.2:-66541832 None:intergenic
AATGCATTGTTTCGGATGAA+TGG 0.434128 1.2:+66542165 MS.gene20706:CDS
CTACTCTTTGGTGGACTAAA+CGG 0.434895 1.2:+66541913 MS.gene20706:CDS
AAACTTGAGAGAAAAGCTAT+TGG 0.442825 1.2:-66541016 None:intergenic
GGACACAATCAACTACTCTT+TGG 0.443826 1.2:+66541901 MS.gene20706:CDS
TAAGGCGAATGGCGTTGGTT+CGG 0.445184 1.2:+66542191 MS.gene20706:CDS
AATGAGAGATGAAACTGAAA+TGG 0.445715 1.2:+66541261 MS.gene20706:CDS
CGTTCATGGCGTTGCGAAGA+CGG 0.447206 1.2:-66541873 None:intergenic
TCACTAGAGACATCAGATAT+AGG 0.453855 1.2:-66540001 None:intergenic
TAATCTTGATTTCTTCAAAA+GGG 0.457630 1.2:+66540805 MS.gene20706:CDS
TGACGCCTTCTCTTTGGAAA+AGG 0.458709 1.2:-66542225 None:intergenic
ACTTTGATGGAAACAAGTTT+GGG 0.459297 1.2:-66541378 None:intergenic
ATAGGTTCATCCAAACATAA+AGG 0.461835 1.2:+66541720 MS.gene20706:intron
TGAGAGACTACCAGTTCAAA+TGG 0.462996 1.2:+66541822 MS.gene20706:CDS
CCTAGCTGAATAATTGCATA+TGG 0.465183 1.2:-66539963 None:intergenic
CAACGTTCAGGCAGTGGTGC+AGG 0.480962 1.2:+66541946 MS.gene20706:CDS
TCTGATTTGGAGAGAAGGAT+TGG 0.486879 1.2:+66541082 MS.gene20706:CDS
TTCCCTCAACGTTCAGGCAG+TGG 0.490228 1.2:+66541940 MS.gene20706:CDS
TAACTTGGAGGAGGAGGATG+AGG 0.491065 1.2:+66541228 MS.gene20706:CDS
TTTATTTGCAATGAAATCAA+AGG 0.491368 1.2:+66540829 MS.gene20706:CDS
ACTGATGCATAATTATCTCT+TGG 0.494221 1.2:-66541985 None:intergenic
CACCACTGCCTGAACGTTGA+GGG 0.499411 1.2:-66541942 None:intergenic
GATTTAAAGAGCTTATTAGC+AGG 0.501395 1.2:-66542117 None:intergenic
GCATCTTAGCATAGCAACAT+TGG 0.504960 1.2:-66540474 None:intergenic
GGTATTAAGGCGAATGGCGT+TGG 0.505217 1.2:+66542186 MS.gene20706:CDS
TTATTTGCAATGAAATCAAA+GGG 0.505377 1.2:+66540830 MS.gene20706:CDS
CATTGATTAATCTGTTAACA+AGG 0.513704 1.2:-66540655 None:intergenic
AACTCAATCAAGGAAAAGTC+AGG 0.517070 1.2:+66540991 MS.gene20706:CDS
GTTTACTACCAACTCAATCA+AGG 0.523450 1.2:+66540981 MS.gene20706:CDS
CCATATGCAATTATTCAGCT+AGG 0.523686 1.2:+66539963 MS.gene20706:CDS
TCCACACCACCTGGTACACT+AGG 0.525740 1.2:-66540395 None:intergenic
ATAAGTTTCCCTAGTGTACC+AGG 0.527998 1.2:+66540386 MS.gene20706:CDS
ATGGCGTTGGTTCGGAGAGT+CGG 0.532835 1.2:+66542199 MS.gene20706:CDS
CCCTAGTGTACCAGGTGGTG+TGG 0.534693 1.2:+66540394 MS.gene20706:CDS
TTACTTCGAGTTTCAGCTCA+CGG 0.538154 1.2:-66542020 None:intergenic
GAATGTCTTCAAGAATTGCT+TGG 0.538738 1.2:-66541117 None:intergenic
TAAAGGAGTCAGAGCGATAC+CGG 0.540199 1.2:+66541737 MS.gene20706:CDS
GTAGATCTGATTTGGAGAGA+AGG 0.543336 1.2:+66541077 MS.gene20706:CDS
TTCATGCTTTGCACTTCACA+AGG 0.544906 1.2:+66540045 MS.gene20706:CDS
TGAAACACCTTCAGAATGGT+GGG 0.545838 1.2:+66540763 MS.gene20706:CDS
CACAATCAACTACTCTTTGG+TGG 0.547927 1.2:+66541904 MS.gene20706:CDS
TATCATAGATAGTACTGCTA+TGG 0.551886 1.2:-66541171 None:intergenic
TCAAATGCCTCCACACCACC+TGG 0.560746 1.2:-66540404 None:intergenic
TTTCCTCTAGTTTCTAGGAG+TGG 0.568812 1.2:+66540314 MS.gene20706:CDS
CGCAACGCCATGAACGGAGG+AGG 0.569820 1.2:+66541880 MS.gene20706:CDS
CTGAAACACCTTCAGAATGG+TGG 0.572260 1.2:+66540762 MS.gene20706:CDS
CGTCTTCGCAACGCCATGAA+CGG 0.573152 1.2:+66541874 MS.gene20706:CDS
TAGTGTACCAGGTGGTGTGG+AGG 0.580110 1.2:+66540397 MS.gene20706:CDS
AACCTGAAACACCTTCAGAA+TGG 0.581115 1.2:+66540759 MS.gene20706:CDS
TAATCTCTTCAGAAATTGGT+AGG 0.585593 1.2:-66540631 None:intergenic
CATGCGCGAATTGTAAGCGA+TGG 0.589747 1.2:+66541430 MS.gene20706:CDS
AATTCTCTTCAAGTAGTCAA+AGG 0.596034 1.2:+66540899 MS.gene20706:CDS
ATGAATGGTATTAAGGCGAA+TGG 0.597917 1.2:+66542180 MS.gene20706:CDS
GCACCACTGCCTGAACGTTG+AGG 0.599275 1.2:-66541943 None:intergenic
ATAATTGCATATGGTAGACA+TGG 0.603537 1.2:-66539954 None:intergenic
CTTCCACTCCTAGAAACTAG+AGG 0.616740 1.2:-66540317 None:intergenic
AGTTTCACAACGGTGAAGAA+CGG 0.627577 1.2:-66540606 None:intergenic
AACTAGTTATTGTTTCAACA+AGG 0.630313 1.2:-66540961 None:intergenic
CCACACCACCTGGTACACTA+GGG 0.639625 1.2:-66540394 None:intergenic
CTTCGCAACGCCATGAACGG+AGG 0.645261 1.2:+66541877 MS.gene20706:CDS
CGAATCATATCTAAAAGAGA+CGG 0.649883 1.2:+66540556 MS.gene20706:CDS
GAAACACCTTCAGAATGGTG+GGG 0.659150 1.2:+66540764 MS.gene20706:CDS
ATGATACAGACTCAATTGTG+AGG 0.686110 1.2:+66541188 MS.gene20706:CDS
TTGGTAGGAGAGTTTCACAA+CGG 0.732412 1.2:-66540616 None:intergenic
AGTTTCCCTAGTGTACCAGG+TGG 0.760455 1.2:+66540389 MS.gene20706:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCATAATTTTATCATTA+AGG - chr1.2:66540211-66540230 None:intergenic 10.0%
!!! TTATTTTGATATAAACTTGT+TGG - chr1.2:66541148-66541167 None:intergenic 15.0%
!! ATTAAGCTTACTTAATTTCT+TGG - chr1.2:66542148-66542167 None:intergenic 20.0%
!! TAATCTTGATTTCTTCAAAA+GGG + chr1.2:66540805-66540824 MS.gene20706:CDS 20.0%
!! TATGCAATTACATAAAAGAA+AGG - chr1.2:66540086-66540105 None:intergenic 20.0%
!! TTAATCTTGATTTCTTCAAA+AGG + chr1.2:66540804-66540823 MS.gene20706:CDS 20.0%
!! TTATTTGCAATGAAATCAAA+GGG + chr1.2:66540830-66540849 MS.gene20706:CDS 20.0%
!! TTTATTTGCAATGAAATCAA+AGG + chr1.2:66540829-66540848 MS.gene20706:CDS 20.0%
!!! AGAATTTTTGAAGAAACAAA+GGG + chr1.2:66540937-66540956 MS.gene20706:CDS 20.0%
!!! CTTCATGTTTTCTTTAAAAA+TGG + chr1.2:66540236-66540255 MS.gene20706:intron 20.0%
!!! CTTTGAAAAATAAAACTCTT+AGG + chr1.2:66540127-66540146 MS.gene20706:intron 20.0%
!!! TAGAATTTTTGAAGAAACAA+AGG + chr1.2:66540936-66540955 MS.gene20706:CDS 20.0%
!!! TGATTTTAGAAGGAAAATTA+TGG - chr1.2:66540736-66540755 None:intergenic 20.0%
!!! TTTTTCCATTTTTCTTAACT+TGG + chr1.2:66541213-66541232 MS.gene20706:CDS 20.0%
! AAAACAAAGCTCAATTCTTA+AGG + chr1.2:66541312-66541331 MS.gene20706:CDS 25.0%
! AACTAGTTATTGTTTCAACA+AGG - chr1.2:66540964-66540983 None:intergenic 25.0%
! AAGATAATTGTCAAGCAATT+TGG - chr1.2:66541341-66541360 None:intergenic 25.0%
!! CATTGATTAATCTGTTAACA+AGG - chr1.2:66540658-66540677 None:intergenic 25.0%
!! CTTTTAGATATCAAATTGTC+CGG - chr1.2:66541534-66541553 None:intergenic 25.0%
!! TTCTGTCATTTCTAGAAAAT+GGG - chr1.2:66540504-66540523 None:intergenic 25.0%
!!! TTAAGAATTGAGCTTTGTTT+TGG - chr1.2:66541313-66541332 None:intergenic 25.0%
AAACTTGAGAGAAAAGCTAT+TGG - chr1.2:66541019-66541038 None:intergenic 30.0%
AACTTTGATGGAAACAAGTT+TGG - chr1.2:66541382-66541401 None:intergenic 30.0%
AATGAGAGATGAAACTGAAA+TGG + chr1.2:66541261-66541280 MS.gene20706:CDS 30.0%
AATTCTCTTCAAGTAGTCAA+AGG + chr1.2:66540899-66540918 MS.gene20706:CDS 30.0%
ACTGATGCATAATTATCTCT+TGG - chr1.2:66541988-66542007 None:intergenic 30.0%
ACTTTGATGGAAACAAGTTT+GGG - chr1.2:66541381-66541400 None:intergenic 30.0%
ATAGGTTCATCCAAACATAA+AGG + chr1.2:66541720-66541739 MS.gene20706:intron 30.0%
ATCTTCACTTTATGATTGTC+TGG + chr1.2:66541491-66541510 MS.gene20706:intron 30.0%
CGAATCATATCTAAAAGAGA+CGG + chr1.2:66540556-66540575 MS.gene20706:CDS 30.0%
GATTTAAAGAGCTTATTAGC+AGG - chr1.2:66542120-66542139 None:intergenic 30.0%
GTAAGCTTAATGCATTGTTT+CGG + chr1.2:66542157-66542176 MS.gene20706:CDS 30.0%
TAATCTCTTCAGAAATTGGT+AGG - chr1.2:66540634-66540653 None:intergenic 30.0%
TACTTCTTGTAGATCTGATT+TGG + chr1.2:66541069-66541088 MS.gene20706:CDS 30.0%
TATCATAGATAGTACTGCTA+TGG - chr1.2:66541174-66541193 None:intergenic 30.0%
TTTCAGTTTCATCTCTCATT+CGG - chr1.2:66541261-66541280 None:intergenic 30.0%
! ATCAGTTGATATCTTCCTTA+AGG + chr1.2:66541462-66541481 MS.gene20706:CDS 30.0%
! ATGCTTCTTGTGATAGTTTT+TGG - chr1.2:66541783-66541802 None:intergenic 30.0%
! CGTCTCTTTTAGATATGATT+CGG - chr1.2:66540558-66540577 None:intergenic 30.0%
! CTTCTGTCATTTCTAGAAAA+TGG - chr1.2:66540505-66540524 None:intergenic 30.0%
! GATTTTGTACTTCTTGTCAT+AGG + chr1.2:66541702-66541721 MS.gene20706:intron 30.0%
! TCAGGTTCAATGATTTTAGA+AGG - chr1.2:66540746-66540765 None:intergenic 30.0%
!! TTCCATTTTTCTTAACTTGG+AGG + chr1.2:66541216-66541235 MS.gene20706:CDS 30.0%
!!! TGATAGTTTTTGGCAATCTA+TGG - chr1.2:66541773-66541792 None:intergenic 30.0%
AACTCAATCAAGGAAAAGTC+AGG + chr1.2:66540991-66541010 MS.gene20706:CDS 35.0%
AAGGTAATACTCATGCATGT+TGG - chr1.2:66540192-66540211 None:intergenic 35.0%
AATGCATTGTTTCGGATGAA+TGG + chr1.2:66542165-66542184 MS.gene20706:CDS 35.0%
ACAGATTCACAAGGACTAAA+TGG + chr1.2:66541616-66541635 MS.gene20706:intron 35.0%
ACCTAGTTAACAGATTCACA+AGG + chr1.2:66541607-66541626 MS.gene20706:intron 35.0%
AGGCTAATCTCTTCAGAAAT+TGG - chr1.2:66540638-66540657 None:intergenic 35.0%
AGTGAAGATTGCTTACCTTA+AGG - chr1.2:66541480-66541499 None:intergenic 35.0%
ATGAATGGTATTAAGGCGAA+TGG + chr1.2:66542180-66542199 MS.gene20706:CDS 35.0%
ATGATACAGACTCAATTGTG+AGG + chr1.2:66541188-66541207 MS.gene20706:CDS 35.0%
CCATATGCAATTATTCAGCT+AGG + chr1.2:66539963-66539982 MS.gene20706:CDS 35.0%
CCTAGCTGAATAATTGCATA+TGG - chr1.2:66539966-66539985 None:intergenic 35.0%
CTAGCTGCAAAATTCTGTTA+TGG + chr1.2:66540428-66540447 MS.gene20706:CDS 35.0%
CTCCTCCAAGTTAAGAAAAA+TGG - chr1.2:66541221-66541240 None:intergenic 35.0%
GAATGTCTTCAAGAATTGCT+TGG - chr1.2:66541120-66541139 None:intergenic 35.0%
GCAAGTGAAATGAACTTTGA+TGG - chr1.2:66541394-66541413 None:intergenic 35.0%
GTTTACTACCAACTCAATCA+AGG + chr1.2:66540981-66541000 MS.gene20706:CDS 35.0%
GTTTCGGATGAATGGTATTA+AGG + chr1.2:66542173-66542192 MS.gene20706:CDS 35.0%
TCACTAGAGACATCAGATAT+AGG - chr1.2:66540004-66540023 None:intergenic 35.0%
TCCTTGTGAATCTGTTAACT+AGG - chr1.2:66541611-66541630 None:intergenic 35.0%
! AGTGATCTTAGCATCGAAAT+AGG + chr1.2:66540019-66540038 MS.gene20706:CDS 35.0%
! TACAGTTTCCTCTAGTTTCT+AGG + chr1.2:66540309-66540328 MS.gene20706:intron 35.0%
! TGACTTTTCCTTGATTGAGT+TGG - chr1.2:66540992-66541011 None:intergenic 35.0%
! TTTCTAGGAGTGGAAGAATT+AGG + chr1.2:66540324-66540343 MS.gene20706:CDS 35.0%
!! AATCTATGGTTTTGCATAGC+CGG - chr1.2:66541759-66541778 None:intergenic 35.0%
!! CATTTTTCTTAACTTGGAGG+AGG + chr1.2:66541219-66541238 MS.gene20706:CDS 35.0%
!! GTTTATGATTTTGATAGCCC+AGG + chr1.2:66541283-66541302 MS.gene20706:CDS 35.0%
AACCTGAAACACCTTCAGAA+TGG + chr1.2:66540759-66540778 MS.gene20706:CDS 40.0%
AGTTTCACAACGGTGAAGAA+CGG - chr1.2:66540609-66540628 None:intergenic 40.0%
ATAAGTTTCCCTAGTGTACC+AGG + chr1.2:66540386-66540405 MS.gene20706:CDS 40.0%
CACAATCAACTACTCTTTGG+TGG + chr1.2:66541904-66541923 MS.gene20706:CDS 40.0%
CAGCTAGGTTTGTGTATTAC+AGG + chr1.2:66539978-66539997 MS.gene20706:CDS 40.0%
GCATCTTAGCATAGCAACAT+TGG - chr1.2:66540477-66540496 None:intergenic 40.0%
GGACACAATCAACTACTCTT+TGG + chr1.2:66541901-66541920 MS.gene20706:CDS 40.0%
TGAAACACCTTCAGAATGGT+GGG + chr1.2:66540763-66540782 MS.gene20706:CDS 40.0%
TGAGAGACTACCAGTTCAAA+TGG + chr1.2:66541822-66541841 MS.gene20706:CDS 40.0%
TGCAAAGAGCAACTTACTTC+TGG + chr1.2:66540695-66540714 MS.gene20706:CDS 40.0%
TTCATGCTTTGCACTTCACA+AGG + chr1.2:66540045-66540064 MS.gene20706:CDS 40.0%
TTGGTAGGAGAGTTTCACAA+CGG - chr1.2:66540619-66540638 None:intergenic 40.0%
! ACTTGTACTGCCATTTGAAC+TGG - chr1.2:66541835-66541854 None:intergenic 40.0%
! GAGAATGAGACTCACTGATT+TGG + chr1.2:66542050-66542069 MS.gene20706:CDS 40.0%
! GGTTTCCTTTTCCAAAGAGA+AGG + chr1.2:66542220-66542239 MS.gene20706:CDS 40.0%
! GTCCGGTTCTGATAATTTGA+GGG - chr1.2:66541517-66541536 None:intergenic 40.0%
! TCGATCACATTAGTCTCTGT+GGG + chr1.2:66541572-66541591 MS.gene20706:intron 40.0%
! TCGCGTCAACAAGTTTTTCT+CGG - chr1.2:66540534-66540553 None:intergenic 40.0%
! TGTCCGGTTCTGATAATTTG+AGG - chr1.2:66541518-66541537 None:intergenic 40.0%
! TTACTTCGAGTTTCAGCTCA+CGG - chr1.2:66542023-66542042 None:intergenic 40.0%
! TTTCCTCTAGTTTCTAGGAG+TGG + chr1.2:66540314-66540333 MS.gene20706:CDS 40.0%
!! CTACTCTTTGGTGGACTAAA+CGG + chr1.2:66541913-66541932 MS.gene20706:CDS 40.0%
!! GTAGATCTGATTTGGAGAGA+AGG + chr1.2:66541077-66541096 MS.gene20706:CDS 40.0%
!! TCTGATTTGGAGAGAAGGAT+TGG + chr1.2:66541082-66541101 MS.gene20706:CDS 40.0%
!! TTTTCTTAACTTGGAGGAGG+AGG + chr1.2:66541222-66541241 MS.gene20706:CDS 40.0%
!!! AGCTTTGTTTTGGAGATCCT+GGG - chr1.2:66541303-66541322 None:intergenic 40.0%
ACTGAATGACGCCTTCTCTT+TGG - chr1.2:66542234-66542253 None:intergenic 45.0%
CGCTCTGACTCCTTTATGTT+TGG - chr1.2:66541733-66541752 None:intergenic 45.0%
CTGAAACACCTTCAGAATGG+TGG + chr1.2:66540762-66540781 MS.gene20706:CDS 45.0%
CTTACAATTCGCGCATGATC+AGG - chr1.2:66541427-66541446 None:intergenic 45.0%
CTTCCACTCCTAGAAACTAG+AGG - chr1.2:66540320-66540339 None:intergenic 45.0%
GAAACACCTTCAGAATGGTG+GGG + chr1.2:66540764-66540783 MS.gene20706:CDS 45.0%
GGCCCTCAAATTATCAGAAC+CGG + chr1.2:66541512-66541531 MS.gene20706:intron 45.0%
TAAAGGAGTCAGAGCGATAC+CGG + chr1.2:66541737-66541756 MS.gene20706:CDS 45.0%
! CTCGATCACATTAGTCTCTG+TGG + chr1.2:66541571-66541590 MS.gene20706:intron 45.0%
! GATTTTCCCCACCATTCTGA+AGG - chr1.2:66540773-66540792 None:intergenic 45.0%
! GTCTCTCATTTTGTGCTGCA+TGG - chr1.2:66541810-66541829 None:intergenic 45.0%
! TGACGCCTTCTCTTTGGAAA+AGG - chr1.2:66542228-66542247 None:intergenic 45.0%
!! CACCATTCTGAAGGTGTTTC+AGG - chr1.2:66540764-66540783 None:intergenic 45.0%
!!! GAGCTTTGTTTTGGAGATCC+TGG - chr1.2:66541304-66541323 None:intergenic 45.0%
AGTTTCCCTAGTGTACCAGG+TGG + chr1.2:66540389-66540408 MS.gene20706:CDS 50.0%
ATTGTGTCCTCCTCCGTTCA+TGG - chr1.2:66541890-66541909 None:intergenic 50.0%
CATGCGCGAATTGTAAGCGA+TGG + chr1.2:66541430-66541449 MS.gene20706:CDS 50.0%
GGTATTAAGGCGAATGGCGT+TGG + chr1.2:66542186-66542205 MS.gene20706:CDS 50.0%
GGTCAATTCCCTCAACGTTC+AGG + chr1.2:66541934-66541953 MS.gene20706:CDS 50.0%
TAACTTGGAGGAGGAGGATG+AGG + chr1.2:66541228-66541247 MS.gene20706:CDS 50.0%
!! TAAGGCGAATGGCGTTGGTT+CGG + chr1.2:66542191-66542210 MS.gene20706:CDS 50.0%
CACCACTGCCTGAACGTTGA+GGG - chr1.2:66541945-66541964 None:intergenic 55.0%
CCACACCACCTGGTACACTA+GGG - chr1.2:66540397-66540416 None:intergenic 55.0%
CGTCTTCGCAACGCCATGAA+CGG + chr1.2:66541874-66541893 MS.gene20706:CDS 55.0%
CGTTCATGGCGTTGCGAAGA+CGG - chr1.2:66541876-66541895 None:intergenic 55.0%
TAGTGTACCAGGTGGTGTGG+AGG + chr1.2:66540397-66540416 MS.gene20706:CDS 55.0%
TCAAATGCCTCCACACCACC+TGG - chr1.2:66540407-66540426 None:intergenic 55.0%
TCCACACCACCTGGTACACT+AGG - chr1.2:66540398-66540417 None:intergenic 55.0%
TTCCCTCAACGTTCAGGCAG+TGG + chr1.2:66541940-66541959 MS.gene20706:CDS 55.0%
!! ATGGCGTTGGTTCGGAGAGT+CGG + chr1.2:66542199-66542218 MS.gene20706:CDS 55.0%
CAACGTTCAGGCAGTGGTGC+AGG + chr1.2:66541946-66541965 MS.gene20706:CDS 60.0%
CCCTAGTGTACCAGGTGGTG+TGG + chr1.2:66540394-66540413 MS.gene20706:CDS 60.0%
CTTCGCAACGCCATGAACGG+AGG + chr1.2:66541877-66541896 MS.gene20706:CDS 60.0%
GCACCACTGCCTGAACGTTG+AGG - chr1.2:66541946-66541965 None:intergenic 60.0%
TCAGGCAGTGGTGCAGGAAG+TGG + chr1.2:66541952-66541971 MS.gene20706:CDS 60.0%
CGCAACGCCATGAACGGAGG+AGG + chr1.2:66541880-66541899 MS.gene20706:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 66539956 66542260 66539956 ID=MS.gene20706
chr1.2 mRNA 66539956 66542260 66539956 ID=MS.gene20706.t1;Parent=MS.gene20706
chr1.2 exon 66539956 66540066 66539956 ID=MS.gene20706.t1.exon1;Parent=MS.gene20706.t1
chr1.2 CDS 66539956 66540066 66539956 ID=cds.MS.gene20706.t1;Parent=MS.gene20706.t1
chr1.2 exon 66540314 66541483 66540314 ID=MS.gene20706.t1.exon2;Parent=MS.gene20706.t1
chr1.2 CDS 66540314 66541483 66540314 ID=cds.MS.gene20706.t1;Parent=MS.gene20706.t1
chr1.2 exon 66541724 66542260 66541724 ID=MS.gene20706.t1.exon3;Parent=MS.gene20706.t1
chr1.2 CDS 66541724 66542260 66541724 ID=cds.MS.gene20706.t1;Parent=MS.gene20706.t1
Gene Sequence

>MS.gene20706

ATGTCTACCATATGCAATTATTCAGCTAGGTTTGTGTATTACAGGCCTATATCTGATGTCTCTAGTGATCTTAGCATCGAAATAGGAGCTTCATGCTTTGCACTTCACAAGGTAAAAAGAAAAGCTTCCTTTCTTTTATGTAATTGCATATTCAAATTTCAGCATAGTGTTCTTTGAAAAATAAAACTCTTAGGTACAAATATATAAATGACATTTTTTCTGTCTAGAATATGCCAACATGCATGAGTATTACCTTAATGATAAAATTATGAAAATATTGCTTCATGTTTTCTTTAAAAATGGAAAGAGAGACAATATTGAAGTACTACTAAGTTGTTTGCAACTGTTTTTTCTACAGTTTCCTCTAGTTTCTAGGAGTGGAAGAATTAGGAAACTATTGTTAGAATCAAAAGATTCTAAAGTTTCGCGCATAAGTTTCCCTAGTGTACCAGGTGGTGTGGAGGCATTTGAGCTAGCTGCAAAATTCTGTTATGGAATTAGCATTGAGTTTACTCTCTCCAATGTTGCTATGCTAAGATGCATAGCCCATTTTCTAGAAATGACAGAAGAATTTGCCGAGAAAAACTTGTTGACGCGAGCCGAATCATATCTAAAAGAGACGGTGCTTTCAAACACAGCAAACTCAATATCCGTTCTTCACCGTTGTGAAACTCTCCTACCAATTTCTGAAGAGATTAGCCTTGTTAACAGATTAATCAATGCAATTTCAAGTAATGTATGCAAAGAGCAACTTACTTCTGGTTTGCAAAAACTAGACCATAATTTTCCTTCTAAAATCATTGAACCTGAAACACCTTCAGAATGGTGGGGAAAATCACTTACTGTTCTTAATCTTGATTTCTTCAAAAGGGTTTTATTTGCAATGAAATCAAAGGGACTTAAACAAGATATTATCAGCAAAATTTTGATAAACTATGCACATAATTCTCTTCAAGTAGTCAAAGGAAATTTACTTGATTTAGAATTTTTGAAGAAACAAAGGGTCCTTGTTGAAACAATAACTAGTTTACTACCAACTCAATCAAGGAAAAGTCAGGTTCCAATAGCTTTTCTCTCAAGTTTGTTGAAATCTGCAATAGCAGCATCAGTATCTACTTCTTGTAGATCTGATTTGGAGAGAAGGATTGGTCTACAACTTGACCAAGCAATTCTTGAAGACATTCTGATTCCAACAAGTTTATATCAAAATAATAACCATAGCAGTACTATCTATGATACAGACTCAATTGTGAGGATTTTTTCCATTTTTCTTAACTTGGAGGAGGAGGATGAGGACGATAGCCGAATGAGAGATGAAACTGAAATGGTTTATGATTTTGATAGCCCAGGATCTCCAAAACAAAGCTCAATTCTTAAGGTATCCAAATTGCTTGACAATTATCTTGCTGAAGTAGCTTTAGACCCAAACTTGTTTCCATCAAAGTTCATTTCACTTGCTGAGTTACTTCCTGATCATGCGCGAATTGTAAGCGATGGACTCTATAGATCAGTTGATATCTTCCTTAAGGTAAGCAATCTTCACTTTATGATTGTCTGGCCCTCAAATTATCAGAACCGGACAATTTGATATCTAAAAGCGATGAAATTGAAAATTCTCGATCACATTAGTCTCTGTGGGACAATTTTATATACCTAGTTAACAGATTCACAAGGACTAAATGGTCGAGAATTTTTCAAATTCAATGACTATACTATTCTGAAATATTAACAATTTCACGATAAACTGATTTTGTACTTCTTGTCATAGGTTCATCCAAACATAAAGGAGTCAGAGCGATACCGGCTATGCAAAACCATAGATTGCCAAAAACTATCACAAGAAGCATGCAGCCATGCAGCACAAAATGAGAGACTACCAGTTCAAATGGCAGTACAAGTTCTATACTTTGAACAAATCCGTCTTCGCAACGCCATGAACGGAGGAGGACACAATCAACTACTCTTTGGTGGACTAAACGGTCAATTCCCTCAACGTTCAGGCAGTGGTGCAGGAAGTGGAGCAATGTCGCCAAGAGATAATTATGCATCAGTTAGAAGAGAAAACCGTGAGCTGAAACTCGAAGTAACAAGAATGAGAATGAGACTCACTGATTTGGAAAAAGATCATGTTTCTATGAAACAAGAGCTTGTTAAGTCTCATCCTGCTAATAAGCTCTTTAAATCATTCACCAAGAAATTAAGTAAGCTTAATGCATTGTTTCGGATGAATGGTATTAAGGCGAATGGCGTTGGTTCGGAGAGTCGGTTTCCTTTTCCAAAGAGAAGGCGTCATTCAGTTGCTTGA
Protein sequence

>MS.gene20706.t1

MSTICNYSARFVYYRPISDVSSDLSIEIGASCFALHKFPLVSRSGRIRKLLLESKDSKVSRISFPSVPGGVEAFELAAKFCYGISIEFTLSNVAMLRCIAHFLEMTEEFAEKNLLTRAESYLKETVLSNTANSISVLHRCETLLPISEEISLVNRLINAISSNVCKEQLTSGLQKLDHNFPSKIIEPETPSEWWGKSLTVLNLDFFKRVLFAMKSKGLKQDIISKILINYAHNSLQVVKGNLLDLEFLKKQRVLVETITSLLPTQSRKSQVPIAFLSSLLKSAIAASVSTSCRSDLERRIGLQLDQAILEDILIPTSLYQNNNHSSTIYDTDSIVRIFSIFLNLEEEDEDDSRMRDETEMVYDFDSPGSPKQSSILKVSKLLDNYLAEVALDPNLFPSKFISLAELLPDHARIVSDGLYRSVDIFLKVHPNIKESERYRLCKTIDCQKLSQEACSHAAQNERLPVQMAVQVLYFEQIRLRNAMNGGGHNQLLFGGLNGQFPQRSGSGAGSGAMSPRDNYASVRRENRELKLEVTRMRMRLTDLEKDHVSMKQELVKSHPANKLFKSFTKKLSKLNALFRMNGIKANGVGSESRFPFPKRRRHSVA