AlfalfaGEDB | MS.gene21423

M. sativa cultivar XinJiangDaYe / MS.gene21423

KEGG: K11252
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21423.t1	XP_013469298.1	100	137	0	0	1	137	1	137	8.20E-42	179.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21423.t1	Q1S9I9	100.0	91	0	0	47	137	58	148	3.7e-43	175.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21423.t1	I3S6Z4	100.0	137	0	0	1	137	1	137	5.9e-42	179.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene053173	MS.gene21423	0.839389	1.63E-57	-1.69E-46
MS.gene054551	MS.gene21423	0.826976	2.02E-54	-1.69E-46
MS.gene054662	MS.gene21423	0.820466	6.76E-53	-1.69E-46
MS.gene05640	MS.gene21423	0.806084	9.77E-50	-1.69E-46
MS.gene057367	MS.gene21423	0.802416	5.67E-49	-1.69E-46
MS.gene057458	MS.gene21423	0.801253	9.83E-49	-1.69E-46
MS.gene058853	MS.gene21423	0.811771	5.93E-51	-1.69E-46
MS.gene06093	MS.gene21423	0.845467	3.99E-59	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21423.t1	MTR_1g492910	100.000	137	0	0	1	137	1	137	2.30e-95	271
MS.gene21423.t1	MTR_1g090780	99.270	137	1	0	1	137	1	137	1.25e-94	269
MS.gene21423.t1	MTR_4g063240	86.395	147	10	3	1	137	1	147	7.09e-81	234
MS.gene21423.t1	MTR_4g063220	87.162	148	8	3	1	137	1	148	3.56e-77	225
MS.gene21423.t1	MTR_4g063990	87.162	148	8	3	1	137	1	148	3.56e-77	225
MS.gene21423.t1	MTR_4g063200	87.162	148	8	3	1	137	1	148	3.56e-77	225
MS.gene21423.t1	MTR_4g064020	87.162	148	8	3	1	137	1	148	3.56e-77	225
MS.gene21423.t1	MTR_4g071180	87.162	148	8	3	1	137	1	148	3.12e-76	223
MS.gene21423.t1	MTR_4g070240	87.162	148	8	3	1	137	1	148	3.12e-76	223
MS.gene21423.t1	MTR_4g088025	89.510	143	9	3	1	137	1	143	6.36e-76	222
MS.gene21423.t1	MTR_4g070245	85.811	148	10	3	1	137	1	148	1.45e-74	219
MS.gene21423.t1	MTR_4g063275	84.459	148	12	3	1	137	1	148	6.43e-74	217
MS.gene21423.t1	MTR_4g064983	86.111	144	9	3	1	133	1	144	1.53e-72	217
MS.gene21423.t1	MTR_7g099960	95.413	109	4	1	29	137	31	138	2.52e-64	192
MS.gene21423.t1	MTR_1g068600	93.578	109	5	1	29	137	30	136	2.97e-63	189
MS.gene21423.t1	MTR_2g084480	61.798	89	34	0	48	136	136	224	1.85e-37	127
MS.gene21423.t1	MTR_8g069990	69.663	89	24	1	8	96	3	88	7.70e-37	121
MS.gene21423.t1	MTR_4g063270	90.196	51	5	0	75	125	1	51	5.24e-27	95.5
MS.gene21423.t1	MTR_7g114580	90.244	41	4	0	97	137	11	51	5.86e-20	77.8
MS.gene21423.t1	MTR_4g032777	36.364	99	58	1	28	121	12	110	3.88e-13	62.4
MS.gene21423.t1	MTR_2g083800	67.273	55	15	2	8	61	11	63	1.75e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21423.t1	AT2G37470	88.406	138	14	2	1	136	1	138	4.90e-75	219
MS.gene21423.t1	AT3G45980	83.333	150	12	3	1	137	1	150	2.60e-74	218
MS.gene21423.t1	AT2G28720	82.119	151	13	2	1	137	1	151	4.16e-74	218
MS.gene21423.t1	AT3G53650	86.331	139	16	3	1	137	1	138	1.55e-71	211
MS.gene21423.t1	AT1G07790	83.333	150	10	4	1	137	1	148	1.89e-71	211
MS.gene21423.t1	AT5G59910	87.313	134	15	2	6	137	17	150	6.51e-70	207
MS.gene21423.t1	AT3G46030	89.147	129	12	2	11	137	17	145	2.25e-69	206
MS.gene21423.t1	AT5G22880	80.282	142	14	2	10	137	4	145	1.94e-66	198
MS.gene21423.t1	AT5G02570	84.173	139	13	2	1	137	1	132	2.96e-66	197
MS.gene21423.t1	AT3G09480	79.562	137	17	2	1	137	1	126	2.00e-62	187
MS.gene21423.t1	AT1G08170	56.989	93	40	0	42	134	143	235	6.80e-38	129

Find 36 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTTCTCTTCTTCTTCTTAT+CGG	0.211855	1.4:-72043378	None:intergenic
AATTCAAGAGCTAGTGAATT+TGG	0.219657	1.4:-72043666	None:intergenic
GCCGTTCGTCTTGTTCTTCC+TGG	0.234822	1.4:+72043599	MS.gene21423:CDS
CTTGAAGATGTAGATCTTGT+AGG	0.279657	1.4:-72043417	None:intergenic
TCGGTTGAGGATGCTTCCTT+TGG	0.316023	1.4:-72043359	None:intergenic
GCGGGTTTCTTCTCTCCCTT+TGG	0.352007	1.4:-72043278	None:intergenic
TCAGCTGGTGCCTTCTCTGC+AGG	0.357925	1.4:-72043311	None:intergenic
TGGTATCTCAAGCAAGGCTA+TGG	0.409396	1.4:+72043466	MS.gene21423:CDS
CTTCTTCTTCTTATCGGTTG+AGG	0.416596	1.4:-72043372	None:intergenic
AGTGAATTTGGTGACAGCTT+TGG	0.420989	1.4:-72043654	None:intergenic
CTACAAGATCTACATCTTCA+AGG	0.431521	1.4:+72043418	MS.gene21423:CDS
CGGTTGAGGATGCTTCCTTT+GGG	0.432726	1.4:-72043358	None:intergenic
TCCCTCGGAGACAGCGTGCT+TGG	0.436021	1.4:-72043630	None:intergenic
CATCTTCAAGGTTCTCAAGC+AGG	0.470454	1.4:+72043430	MS.gene21423:CDS
TCTGCAGGCTTCTTCTCTGC+GGG	0.470993	1.4:-72043296	None:intergenic
AGAGAAGAAGCCTGCAGAGA+AGG	0.499836	1.4:+72043301	MS.gene21423:CDS
CTCTGCAGGCTTCTTCTCTG+CGG	0.503353	1.4:-72043297	None:intergenic
GGTGACAGCTTTGGTTCCCT+CGG	0.503972	1.4:-72043645	None:intergenic
CTGAATCTCCCGAGAGGTGA+TGG	0.509224	1.4:-72043573	None:intergenic
TCAATCAACAATGGCACCAA+AGG	0.515027	1.4:+72043262	None:intergenic
GGTATCTCAAGCAAGGCTAT+GGG	0.517327	1.4:+72043467	MS.gene21423:CDS
GCGTGCTTGGCAAGTTCACC+AGG	0.518071	1.4:-72043617	None:intergenic
CATCTTTGAGAAGCTCGCTC+AGG	0.523220	1.4:+72043514	MS.gene21423:CDS
TTGCTTGAGATACCAATGTC+AGG	0.545226	1.4:-72043458	None:intergenic
GAAACCTACCATCACCTCTC+GGG	0.549293	1.4:+72043565	MS.gene21423:CDS
AGAAACCTACCATCACCTCT+CGG	0.550335	1.4:+72043564	MS.gene21423:CDS
GCCAAGCACGCTGTCTCCGA+GGG	0.551418	1.4:+72043629	MS.gene21423:CDS
CAATCAACAATGGCACCAAA+GGG	0.556792	1.4:+72043263	None:intergenic
TGCCAAGCACGCTGTCTCCG+AGG	0.601193	1.4:+72043628	MS.gene21423:CDS
AAGCAGGTTCATCCTGACAT+TGG	0.605433	1.4:+72043446	MS.gene21423:CDS
TGACATTGGTATCTCAAGCA+AGG	0.620719	1.4:+72043460	MS.gene21423:CDS
ACCAGGAAGAACAAGACGAA+CGG	0.639588	1.4:-72043600	None:intergenic
GTATCTCAAGCAAGGCTATG+GGG	0.662407	1.4:+72043468	MS.gene21423:CDS
AGCAGAGAAGAAGATCCCAA+AGG	0.665852	1.4:+72043343	MS.gene21423:CDS
ATCTCCCGAGAGGTGATGGT+AGG	0.682571	1.4:-72043569	None:intergenic
GGCAGTCTGAATCTCCCGAG+AGG	0.728312	1.4:-72043579	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GTTTCTCTTCTTCTTCTTAT+CGG	-	chr1.4:72043381-72043400	None:intergenic	30.0%
	CTACAAGATCTACATCTTCA+AGG	+	chr1.4:72043418-72043437	MS.gene21423:CDS	35.0%
	CTTGAAGATGTAGATCTTGT+AGG	-	chr1.4:72043420-72043439	None:intergenic	35.0%
	TGACATTGGTATCTCAAGCA+AGG	+	chr1.4:72043460-72043479	MS.gene21423:CDS	40.0%
	TTGCTTGAGATACCAATGTC+AGG	-	chr1.4:72043461-72043480	None:intergenic	40.0%
!	AGTGAATTTGGTGACAGCTT+TGG	-	chr1.4:72043657-72043676	None:intergenic	40.0%
!	CTTCTTCTTCTTATCGGTTG+AGG	-	chr1.4:72043375-72043394	None:intergenic	40.0%
	AAGCAGGTTCATCCTGACAT+TGG	+	chr1.4:72043446-72043465	MS.gene21423:CDS	45.0%
	ACCAGGAAGAACAAGACGAA+CGG	-	chr1.4:72043603-72043622	None:intergenic	45.0%
	AGAAACCTACCATCACCTCT+CGG	+	chr1.4:72043564-72043583	MS.gene21423:CDS	45.0%
	AGCAGAGAAGAAGATCCCAA+AGG	+	chr1.4:72043343-72043362	MS.gene21423:CDS	45.0%
	CATCTTCAAGGTTCTCAAGC+AGG	+	chr1.4:72043430-72043449	MS.gene21423:CDS	45.0%
	GGTATCTCAAGCAAGGCTAT+GGG	+	chr1.4:72043467-72043486	MS.gene21423:CDS	45.0%
	GTATCTCAAGCAAGGCTATG+GGG	+	chr1.4:72043468-72043487	MS.gene21423:CDS	45.0%
	TGGTATCTCAAGCAAGGCTA+TGG	+	chr1.4:72043466-72043485	MS.gene21423:CDS	45.0%
!	TCTGCTTTCGTTTTCTCAGC+TGG	-	chr1.4:72043329-72043348	None:intergenic	45.0%
	AGAGAAGAAGCCTGCAGAGA+AGG	+	chr1.4:72043301-72043320	MS.gene21423:CDS	50.0%
	CGGTTGAGGATGCTTCCTTT+GGG	-	chr1.4:72043361-72043380	None:intergenic	50.0%
	GAAACCTACCATCACCTCTC+GGG	+	chr1.4:72043565-72043584	MS.gene21423:CDS	50.0%
	TCGGTTGAGGATGCTTCCTT+TGG	-	chr1.4:72043362-72043381	None:intergenic	50.0%
!	CATCTTTGAGAAGCTCGCTC+AGG	+	chr1.4:72043514-72043533	MS.gene21423:CDS	50.0%
	CTCTGCAGGCTTCTTCTCTG+CGG	-	chr1.4:72043300-72043319	None:intergenic	55.0%
	CTGAATCTCCCGAGAGGTGA+TGG	-	chr1.4:72043576-72043595	None:intergenic	55.0%
	GCGGGTTTCTTCTCTCCCTT+TGG	-	chr1.4:72043281-72043300	None:intergenic	55.0%
	TCTGCAGGCTTCTTCTCTGC+GGG	-	chr1.4:72043299-72043318	None:intergenic	55.0%
!	ATCTCCCGAGAGGTGATGGT+AGG	-	chr1.4:72043572-72043591	None:intergenic	55.0%
!	GCCGTTCGTCTTGTTCTTCC+TGG	+	chr1.4:72043599-72043618	MS.gene21423:CDS	55.0%
!	GGTGACAGCTTTGGTTCCCT+CGG	-	chr1.4:72043648-72043667	None:intergenic	55.0%
	GCGTGCTTGGCAAGTTCACC+AGG	-	chr1.4:72043620-72043639	None:intergenic	60.0%
	GGCAGTCTGAATCTCCCGAG+AGG	-	chr1.4:72043582-72043601	None:intergenic	60.0%
	TCAGCTGGTGCCTTCTCTGC+AGG	-	chr1.4:72043314-72043333	None:intergenic	60.0%
	GCCAAGCACGCTGTCTCCGA+GGG	+	chr1.4:72043629-72043648	MS.gene21423:CDS	65.0%
	TCCCTCGGAGACAGCGTGCT+TGG	-	chr1.4:72043633-72043652	None:intergenic	65.0%
	TGCCAAGCACGCTGTCTCCG+AGG	+	chr1.4:72043628-72043647	MS.gene21423:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	72043272	72043685	72043272	ID=MS.gene21423
chr1.4	mRNA	72043272	72043685	72043272	ID=MS.gene21423.t1;Parent=MS.gene21423
chr1.4	exon	72043272	72043685	72043272	ID=MS.gene21423.t1.exon1;Parent=MS.gene21423.t1
chr1.4	CDS	72043272	72043685	72043272	ID=cds.MS.gene21423.t1;Parent=MS.gene21423.t1

Gene Sequence

>MS.gene21423

ATGGCACCAAAGGGAGAGAAGAAACCCGCAGAGAAGAAGCCTGCAGAGAAGGCACCAGCTGAGAAAACGAAAGCAGAGAAGAAGATCCCAAAGGAAGCATCCTCAACCGATAAGAAGAAGAAGAGAAACAAGAAGAGCGTTGAAACCTACAAGATCTACATCTTCAAGGTTCTCAAGCAGGTTCATCCTGACATTGGTATCTCAAGCAAGGCTATGGGGATCATGAACAGCTTCATCAATGACATCTTTGAGAAGCTCGCTCAGGAATCATCTAGACTCGCTCGCTACAACAAGAAACCTACCATCACCTCTCGGGAGATTCAGACTGCCGTTCGTCTTGTTCTTCCTGGTGAACTTGCCAAGCACGCTGTCTCCGAGGGAACCAAAGCTGTCACCAAATTCACTAGCTCTTGA

Protein sequence

>MS.gene21423.t1

MAPKGEKKPAEKKPAEKAPAEKTKAEKKIPKEASSTDKKKKRNKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS