AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057458


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057458.t1 MTR_7g059070 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene057458.t1 MTR_5g029820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene057458.t1 MTR_5g029770 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene057458.t1 MTR_4g065990 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene057458.t1 MTR_4g088150 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene057458.t1 MTR_2g035230 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene057458.t1 MTR_8g092720 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene057458.t1 MTR_8g063500 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene057458.t1 MTR_8g103245 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene057458.t1 MTR_8g092820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene057458.t1 MTR_7g013610 100.000 136 0 0 1 136 100 235 1.10e-95 275
MS.gene057458.t1 MTR_4g097175 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene057458.t1 MTR_1g023630 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene057458.t1 MTR_8g061940 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene057458.t1 MTR_8g099430 96.324 136 5 0 1 136 1 136 1.38e-92 264
MS.gene057458.t1 MTR_4g097170 96.324 136 5 0 1 136 42 177 2.29e-91 262
MS.gene057458.t1 MTR_2g082310 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene057458.t1 MTR_2g082340 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene057458.t1 MTR_2g082370 85.616 146 11 1 1 136 1 146 1.74e-82 238
MS.gene057458.t1 MTR_2g082400 84.559 136 19 2 1 135 1 135 6.38e-75 219
MS.gene057458.t1 MTR_7g013600 86.607 112 15 0 25 136 2 113 1.01e-65 195
MS.gene057458.t1 MTR_7g023830 67.164 134 43 1 1 134 1 133 6.48e-56 171
MS.gene057458.t1 MTR_1g023630 73.504 117 15 3 1 111 1 107 3.81e-52 160
MS.gene057458.t1 MTR_5g024630 67.241 116 35 2 1 116 1 113 1.63e-45 144
MS.gene057458.t1 MTR_7g023880 58.647 133 41 3 3 134 18 137 5.46e-43 139
MS.gene057458.t1 MTR_5g024530 68.182 110 12 2 19 128 68 154 4.56e-42 137
MS.gene057458.t1 MTR_0197s0100 100.000 66 0 0 1 66 1 66 1.45e-40 132
MS.gene057458.t1 MTR_8g027840 55.102 98 43 1 37 133 28 125 4.83e-30 105
MS.gene057458.t1 MTR_8g105150 53.846 91 10 1 37 127 11 69 1.43e-25 92.4
MS.gene057458.t1 MTR_8g092740 97.368 38 1 0 99 136 118 155 4.60e-20 80.9
MS.gene057458.t1 MTR_5g089120 76.190 42 10 0 82 123 6 47 6.07e-18 73.6
MS.gene057458.t1 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.76e-15 66.2
MS.gene057458.t1 MTR_4g128040 75.676 37 9 0 79 115 20 56 3.38e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057458.t1 AT5G65360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene057458.t1 AT5G10390 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene057458.t1 AT5G10400 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene057458.t1 AT3G27360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene057458.t1 AT1G09200 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene057458.t1 AT5G10980 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene057458.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene057458.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene057458.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene057458.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene057458.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene057458.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene057458.t1 AT4G40030 97.059 136 4 0 1 136 29 164 6.76e-93 266
MS.gene057458.t1 AT5G65350 94.853 136 7 0 1 136 1 136 5.09e-91 260
MS.gene057458.t1 AT1G75600 93.382 136 9 0 1 136 1 136 1.20e-89 256
MS.gene057458.t1 AT1G13370 93.382 136 9 0 1 136 1 136 5.32e-89 254
MS.gene057458.t1 AT1G19890 89.781 137 13 1 1 136 1 137 1.03e-83 241
MS.gene057458.t1 AT5G12910 71.111 135 34 2 1 135 1 130 9.51e-67 198
MS.gene057458.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene057458.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene057458.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene057458.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110

Find 23 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGATTTCACGAACCAATCTT+TGG 0.247437 7.2:-83799272 None:intergenic
TTCTTCACTCCGCCGGTTGC+CGG 0.267479 7.2:-83799159 None:intergenic
TCACGAAGCGCGACTGTTCC+TGG 0.312619 7.2:-83799198 None:intergenic
AGAAAATCAGCACCGGCAAC+CGG 0.393533 7.2:+83799147 MS.gene057458:CDS
GCTGCTGAAGCGTATCTTGT+TGG 0.411498 7.2:+83799354 MS.gene057458:CDS
GAAGCTTCCATTCCAAAGAT+TGG 0.412914 7.2:+83799260 MS.gene057458:CDS
CTTGCAAGTTGAATATCCTT+AGG 0.413826 7.2:-83799432 None:intergenic
AGAGCACTGAGCTTCTCATT+AGG 0.447434 7.2:+83799238 MS.gene057458:CDS
GTGTGGCTTCTTCACTCCGC+CGG 0.496731 7.2:-83799166 None:intergenic
GAGAGTTACTATTATGCCTA+AGG 0.517685 7.2:+83799416 MS.gene057458:CDS
TCACGAACCAATCTTTGGAA+TGG 0.535409 7.2:-83799267 None:intergenic
ATTGGTTCGTGAAATCGCTC+AGG 0.553548 7.2:+83799278 MS.gene057458:CDS
AGCAGCAAGAAAATCAGCAC+CGG 0.565576 7.2:+83799140 MS.gene057458:CDS
CAGTGCTCTTCTGATACTTG+CGG 0.590815 7.2:-83799224 None:intergenic
CAAACAGCAAGAAAATCCAC+CGG 0.620184 7.2:+83799084 MS.gene057458:CDS
AAATCAGCACCGGCAACCGG+CGG 0.620731 7.2:+83799150 MS.gene057458:CDS
AAGCCACACAGATTCCGTCC+AGG 0.632383 7.2:+83799180 MS.gene057458:CDS
CCACCGGAGGAAAAGCTCCA+CGG 0.637025 7.2:+83799100 MS.gene057458:CDS
CAACTTGCAAGAAGAATCAG+AGG 0.660238 7.2:+83799444 MS.gene057458:CDS
TTGGTGGCGAGTTGTTTCCG+TGG 0.664524 7.2:-83799117 None:intergenic
GAAGCGCGACTGTTCCTGGA+CGG 0.668213 7.2:-83799194 None:intergenic
GTTCCTGGACGGAATCTGTG+TGG 0.677012 7.2:-83799183 None:intergenic
ACAGCAAGAAAATCCACCGG+AGG 0.684797 7.2:+83799087 MS.gene057458:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GAGAGTTACTATTATGCCTA+AGG + chr7.2:83799416-83799435 MS.gene057458:CDS 35.0%
! CTTGCAAGTTGAATATCCTT+AGG - chr7.2:83799435-83799454 None:intergenic 35.0%
!!! GGATTTTCTTGCTGTTTGTT+TGG - chr7.2:83799082-83799101 None:intergenic 35.0%
CAAACAGCAAGAAAATCCAC+CGG + chr7.2:83799084-83799103 MS.gene057458:CDS 40.0%
CAACTTGCAAGAAGAATCAG+AGG + chr7.2:83799444-83799463 MS.gene057458:CDS 40.0%
CGATTTCACGAACCAATCTT+TGG - chr7.2:83799275-83799294 None:intergenic 40.0%
GAAGCTTCCATTCCAAAGAT+TGG + chr7.2:83799260-83799279 MS.gene057458:CDS 40.0%
! TCACGAACCAATCTTTGGAA+TGG - chr7.2:83799270-83799289 None:intergenic 40.0%
!!! TGATTTTCTTGCTGCTTTGG+TGG - chr7.2:83799136-83799155 None:intergenic 40.0%
!!! TGCTGATTTTCTTGCTGCTT+TGG - chr7.2:83799139-83799158 None:intergenic 40.0%
AGCAGCAAGAAAATCAGCAC+CGG + chr7.2:83799140-83799159 MS.gene057458:CDS 45.0%
CAGTGCTCTTCTGATACTTG+CGG - chr7.2:83799227-83799246 None:intergenic 45.0%
!! AGAGCACTGAGCTTCTCATT+AGG + chr7.2:83799238-83799257 MS.gene057458:CDS 45.0%
!! ATTGGTTCGTGAAATCGCTC+AGG + chr7.2:83799278-83799297 MS.gene057458:CDS 45.0%
ACAGCAAGAAAATCCACCGG+AGG + chr7.2:83799087-83799106 MS.gene057458:CDS 50.0%
GCTGCTGAAGCGTATCTTGT+TGG + chr7.2:83799354-83799373 MS.gene057458:CDS 50.0%
! TTTCCGTGGAGCTTTTCCTC+CGG - chr7.2:83799106-83799125 None:intergenic 50.0%
!! AGAAAATCAGCACCGGCAAC+CGG + chr7.2:83799147-83799166 MS.gene057458:CDS 50.0%
AAGCCACACAGATTCCGTCC+AGG + chr7.2:83799180-83799199 MS.gene057458:CDS 55.0%
GTTCCTGGACGGAATCTGTG+TGG - chr7.2:83799186-83799205 None:intergenic 55.0%
!! TTGGTGGCGAGTTGTTTCCG+TGG - chr7.2:83799120-83799139 None:intergenic 55.0%
CCACCGGAGGAAAAGCTCCA+CGG + chr7.2:83799100-83799119 MS.gene057458:CDS 60.0%
GAAGCGCGACTGTTCCTGGA+CGG - chr7.2:83799197-83799216 None:intergenic 60.0%
GTGTGGCTTCTTCACTCCGC+CGG - chr7.2:83799169-83799188 None:intergenic 60.0%
TCACGAAGCGCGACTGTTCC+TGG - chr7.2:83799201-83799220 None:intergenic 60.0%
! TTCTTCACTCCGCCGGTTGC+CGG - chr7.2:83799162-83799181 None:intergenic 60.0%
!! AAATCAGCACCGGCAACCGG+CGG + chr7.2:83799150-83799169 MS.gene057458:CDS 60.0%
! CCGTGGAGCTTTTCCTCCGG+TGG - chr7.2:83799103-83799122 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 83799069 83799479 83799069 ID=MS.gene057458
chr7.2 mRNA 83799069 83799479 83799069 ID=MS.gene057458.t1;Parent=MS.gene057458
chr7.2 exon 83799069 83799479 83799069 ID=MS.gene057458.t1.exon1;Parent=MS.gene057458.t1
chr7.2 CDS 83799069 83799479 83799069 ID=cds.MS.gene057458.t1;Parent=MS.gene057458.t1
Gene Sequence

>MS.gene057458

ATGGCGCGTACCAAACAAACAGCAAGAAAATCCACCGGAGGAAAAGCTCCACGGAAACAACTCGCCACCAAAGCAGCAAGAAAATCAGCACCGGCAACCGGCGGAGTGAAGAAGCCACACAGATTCCGTCCAGGAACAGTCGCGCTTCGTGAGATCCGCAAGTATCAGAAGAGCACTGAGCTTCTCATTAGGAAGCTTCCATTCCAAAGATTGGTTCGTGAAATCGCTCAGGATTTCAAAACTGATCTTCGATTTCAGAGTAGTGCTGTTTCTGCTCTTCAAGAAGCTGCTGAAGCGTATCTTGTTGGTTTGTTTGAAGATACGAATCTTTGTGCTATTCATGCTAAGAGAGTTACTATTATGCCTAAGGATATTCAACTTGCAAGAAGAATCAGAGGCGAAAGAGCTTAG

Protein sequence

>MS.gene057458.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA