AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21692

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21692.t1 MTR_5g008790 92.727 165 9 2 1 165 1 162 1.54e-103 293
MS.gene21692.t1 MTR_1g014860 43.810 105 53 2 5 105 8 110 1.93e-24 94.7
MS.gene21692.t1 MTR_1g014820 44.118 102 54 2 5 105 13 112 1.47e-23 93.6
MS.gene21692.t1 MTR_3g102100 40.800 125 63 3 17 134 43 163 2.46e-23 90.9
MS.gene21692.t1 MTR_1g014800 45.833 96 46 2 17 108 29 122 4.34e-23 91.3
MS.gene21692.t1 MTR_5g016750 46.392 97 45 3 17 110 23 115 6.67e-23 89.7
MS.gene21692.t1 MTR_1g019110 47.475 99 45 2 17 110 42 138 1.36e-22 89.0
MS.gene21692.t1 MTR_2g101340 52.874 87 36 2 12 98 34 115 1.75e-22 89.4
MS.gene21692.t1 MTR_3g105510 44.792 96 50 2 11 105 29 122 2.16e-22 90.1
MS.gene21692.t1 MTR_1g014780 40.952 105 56 2 5 105 24 126 2.34e-22 89.7
MS.gene21692.t1 MTR_3g110205 39.496 119 66 3 12 129 36 149 2.45e-21 85.9
MS.gene21692.t1 MTR_8g102800 52.326 86 36 2 13 98 31 111 4.17e-21 86.3
MS.gene21692.t1 MTR_3g072610 53.684 95 39 2 13 105 19 110 5.44e-21 85.9
MS.gene21692.t1 MTR_5g012910 46.739 92 44 2 7 98 16 102 2.26e-20 83.2
MS.gene21692.t1 MTR_5g058470 49.000 100 50 1 1 100 78 176 3.53e-20 85.1
MS.gene21692.t1 MTR_4g100650 48.276 87 40 2 12 98 67 148 5.97e-20 84.0
MS.gene21692.t1 MTR_6g088405 57.500 80 29 3 19 98 44 118 1.16e-19 82.4
MS.gene21692.t1 MTR_8g027465 41.935 93 52 1 6 98 26 116 1.94e-19 80.9
MS.gene21692.t1 MTR_3g105480 45.349 86 44 2 21 105 33 116 2.78e-19 81.6
MS.gene21692.t1 MTR_7g117690 56.667 60 24 1 19 78 28 85 1.28e-18 78.6
MS.gene21692.t1 MTR_8g013860 44.211 95 48 2 15 109 43 132 1.00e-17 77.0
MS.gene21692.t1 MTR_2g043050 38.542 96 54 2 10 105 10 100 1.20e-17 77.0
MS.gene21692.t1 MTR_7g028925 49.315 73 32 2 5 77 15 82 1.91e-17 73.2
MS.gene21692.t1 MTR_7g046260 45.977 87 42 2 12 98 54 135 2.21e-17 76.6
MS.gene21692.t1 MTR_1g090170 46.250 80 39 2 2 79 133 210 4.00e-17 77.0
MS.gene21692.t1 MTR_1g090170 53.968 63 27 1 17 79 237 297 6.32e-17 77.0
MS.gene21692.t1 MTR_8g006410 47.297 74 37 1 7 80 40 111 6.77e-17 74.7
MS.gene21692.t1 MTR_5g083340 48.649 74 36 1 14 87 80 151 7.06e-17 77.0
MS.gene21692.t1 MTR_4g035430 42.000 100 53 2 6 105 19 113 4.39e-16 72.8
MS.gene21692.t1 MTR_5g062700 36.893 103 59 2 17 119 171 267 8.11e-16 73.9
MS.gene21692.t1 MTR_2g043030 50.847 59 27 1 19 77 19 75 9.32e-16 71.6
MS.gene21692.t1 MTR_3g090760 41.176 102 52 2 14 115 108 201 1.24e-15 73.2
MS.gene21692.t1 MTR_8g090350 52.381 63 28 1 17 79 111 171 1.27e-15 72.8
MS.gene21692.t1 MTR_2g043040 50.847 59 27 1 19 77 19 75 2.14e-15 71.2
MS.gene21692.t1 MTR_3g074130 52.381 63 28 1 17 79 148 208 2.52e-15 72.4
MS.gene21692.t1 MTR_7g020980 55.000 60 25 1 19 78 51 108 7.81e-15 68.6
MS.gene21692.t1 MTR_7g021010 50.725 69 30 2 12 78 42 108 9.24e-15 68.6
MS.gene21692.t1 MTR_7g021010 53.333 60 26 1 19 78 12 69 1.66e-14 67.0
MS.gene21692.t1 MTR_2g085015 42.697 89 44 4 19 105 47 130 2.05e-14 68.6
MS.gene21692.t1 MTR_5g008470 45.205 73 38 1 8 80 64 134 2.22e-14 67.4
MS.gene21692.t1 MTR_2g014300 40.244 82 45 2 6 85 2 81 3.42e-14 67.0
MS.gene21692.t1 MTR_2g090235 48.438 64 31 1 17 80 216 277 3.90e-14 69.3
MS.gene21692.t1 MTR_2g043020 40.580 69 39 1 9 77 9 75 4.16e-14 67.4
MS.gene21692.t1 MTR_5g008590 57.377 61 24 1 19 79 11 69 5.36e-14 66.6
MS.gene21692.t1 MTR_7g081815 49.254 67 31 2 17 82 59 123 7.58e-14 66.6
MS.gene21692.t1 MTR_1g006660 46.739 92 34 3 12 98 40 121 7.78e-14 67.4
MS.gene21692.t1 MTR_2g089170 46.269 67 34 1 13 79 59 123 8.63e-14 66.2
MS.gene21692.t1 MTR_7g081805 49.254 67 31 2 17 82 59 123 1.21e-13 66.2
MS.gene21692.t1 MTR_4g086165 50.000 64 30 1 16 79 79 140 1.24e-13 66.2
MS.gene21692.t1 MTR_4g010640 52.459 61 27 1 17 77 5 63 1.74e-13 65.9
MS.gene21692.t1 MTR_8g092460 49.254 67 32 1 12 78 138 202 1.84e-13 67.0
MS.gene21692.t1 MTR_5g008540 50.000 60 28 1 21 80 26 83 1.84e-13 63.2
MS.gene21692.t1 MTR_4g114570 51.613 62 28 1 16 77 4 63 1.98e-13 65.5
MS.gene21692.t1 MTR_2g103700 50.794 63 29 1 15 77 3 63 2.19e-13 64.7
MS.gene21692.t1 MTR_6g088425 51.724 87 37 3 19 105 49 130 2.33e-13 65.9
MS.gene21692.t1 MTR_7g092190 48.485 66 32 1 14 79 25 88 2.44e-13 66.2
MS.gene21692.t1 MTR_1g101600 53.448 58 25 1 19 76 41 96 2.53e-13 65.1
MS.gene21692.t1 MTR_5g009620 42.857 70 40 0 17 86 110 179 2.98e-13 66.2
MS.gene21692.t1 MTR_5g010940 54.688 64 21 2 19 76 54 115 3.09e-13 65.5
MS.gene21692.t1 MTR_0939s0010 43.284 67 36 1 13 79 59 123 3.51e-13 64.7
MS.gene21692.t1 MTR_4g078710 45.946 74 35 2 11 81 17 88 3.80e-13 65.1
MS.gene21692.t1 MTR_6g465420 56.250 48 21 0 19 66 54 101 4.64e-13 65.5
MS.gene21692.t1 MTR_6g465450 50.000 54 27 0 13 66 48 101 4.71e-13 65.1
MS.gene21692.t1 MTR_1g028560 57.778 45 19 0 17 61 113 157 5.25e-13 65.9
MS.gene21692.t1 MTR_5g010910 54.688 64 21 2 19 76 49 110 5.76e-13 64.7
MS.gene21692.t1 MTR_5g008550 53.226 62 27 1 19 80 21 80 8.37e-13 63.5
MS.gene21692.t1 MTR_5g010930 53.125 64 22 2 19 76 49 110 8.98e-13 63.9
MS.gene21692.t1 MTR_1g074230 42.308 78 42 2 1 77 1 76 9.12e-13 62.4
MS.gene21692.t1 MTR_4g102670 47.143 70 34 2 16 85 12 78 1.04e-12 62.8
MS.gene21692.t1 MTR_4g086220 49.180 61 29 1 19 79 96 154 1.20e-12 63.9
MS.gene21692.t1 MTR_2g078680 36.538 104 53 3 2 94 6 107 1.72e-12 63.2
MS.gene21692.t1 MTR_4g008860 47.541 61 30 1 17 77 6 64 1.73e-12 62.8
MS.gene21692.t1 MTR_4g086160 43.421 76 41 1 2 77 79 152 1.91e-12 63.2
MS.gene21692.t1 MTR_2g019750 46.667 60 32 0 18 77 49 108 2.02e-12 63.9
MS.gene21692.t1 MTR_4g088005 44.776 67 34 2 17 82 55 119 2.06e-12 60.8
MS.gene21692.t1 MTR_5g083330 48.387 62 30 1 18 79 34 93 2.36e-12 63.5
MS.gene21692.t1 MTR_5g082950 54.717 53 24 0 13 65 60 112 2.36e-12 63.9
MS.gene21692.t1 MTR_1g074310 52.459 61 26 2 18 77 20 78 2.66e-12 61.2
MS.gene21692.t1 MTR_2g014340 49.153 59 28 1 19 77 19 75 3.45e-12 62.0
MS.gene21692.t1 MTR_4g119270 45.070 71 33 2 18 82 52 122 3.79e-12 63.2
MS.gene21692.t1 MTR_4g086190 49.180 61 29 1 19 79 96 154 4.21e-12 62.4
MS.gene21692.t1 MTR_3g106290 49.206 63 26 2 17 77 6 64 7.26e-12 60.8
MS.gene21692.t1 MTR_3g053690 40.741 81 41 2 2 77 5 83 8.35e-12 61.2
MS.gene21692.t1 MTR_6g465430 54.167 48 22 0 19 66 54 101 1.02e-11 61.6
MS.gene21692.t1 MTR_6g466130 40.230 87 47 2 19 105 66 147 1.06e-11 61.2
MS.gene21692.t1 MTR_8g069360 42.623 61 35 0 18 78 43 103 1.51e-11 61.6
MS.gene21692.t1 MTR_2g015050 31.690 142 76 5 17 143 132 267 1.83e-11 60.8
MS.gene21692.t1 MTR_1g097190 55.814 43 19 0 19 61 23 65 2.54e-11 58.5
MS.gene21692.t1 MTR_6g465460 54.167 48 22 0 19 66 54 101 2.58e-11 60.5
MS.gene21692.t1 MTR_5g032820 54.545 44 20 0 18 61 45 88 3.32e-11 60.8
MS.gene21692.t1 MTR_6g465530 42.105 76 37 1 19 87 33 108 4.18e-11 58.9
MS.gene21692.t1 MTR_8g099215 57.778 45 19 0 17 61 105 149 4.49e-11 60.1
MS.gene21692.t1 MTR_2g435590 46.667 60 30 1 19 78 96 153 7.51e-11 59.3
MS.gene21692.t1 MTR_6g465850 54.167 48 22 0 19 66 63 110 8.91e-11 59.3
MS.gene21692.t1 MTR_7g084370 56.818 44 19 0 18 61 41 84 9.88e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21692.t1 AT1G22810 44.516 155 64 2 11 165 6 138 3.15e-42 138
MS.gene21692.t1 AT1G71520 45.223 157 56 4 13 165 8 138 4.24e-42 137
MS.gene21692.t1 AT5G67190 47.525 101 49 2 16 116 18 114 7.69e-26 97.4
MS.gene21692.t1 AT3G50260 51.064 94 40 3 17 110 19 106 2.65e-25 95.1
MS.gene21692.t1 AT2G23340 47.423 97 46 2 17 110 26 120 2.93e-25 95.9
MS.gene21692.t1 AT1G21910 45.794 107 52 2 19 121 50 154 1.37e-24 95.5
MS.gene21692.t1 AT4G36900 42.991 107 52 2 16 115 27 131 1.18e-22 89.7
MS.gene21692.t1 AT1G46768 44.144 111 48 4 17 120 29 132 3.61e-22 87.0
MS.gene21692.t1 AT1G19210 62.295 61 21 1 18 78 11 69 3.64e-22 88.2
MS.gene21692.t1 AT1G46768 43.363 113 50 4 15 120 27 132 4.84e-22 87.0
MS.gene21692.t1 AT1G46768 43.363 113 50 4 15 120 27 132 4.84e-22 87.0
MS.gene21692.t1 AT4G06746 45.455 99 50 2 14 110 31 127 1.16e-21 85.9
MS.gene21692.t1 AT4G31060 37.302 126 75 2 5 130 13 134 1.93e-21 86.3
MS.gene21692.t1 AT5G25810 45.745 94 46 2 12 105 29 117 2.35e-21 86.7
MS.gene21692.t1 AT1G74930 46.591 88 42 2 18 105 20 102 3.27e-21 85.9
MS.gene21692.t1 AT1G71450 57.812 64 25 1 19 82 24 85 6.07e-21 84.7
MS.gene21692.t1 AT5G11590 46.465 99 48 2 7 105 39 132 6.43e-21 85.9
MS.gene21692.t1 AT5G21960 37.037 108 63 2 18 125 6 108 1.08e-19 82.4
MS.gene21692.t1 AT4G32800 44.860 107 51 4 6 109 4 105 1.36e-19 82.0
MS.gene21692.t1 AT1G01250 43.333 90 46 2 9 98 35 119 4.36e-19 80.1
MS.gene21692.t1 AT4G16750 60.656 61 22 1 19 79 40 98 6.19e-19 79.7
MS.gene21692.t1 AT3G60490 48.913 92 41 3 8 98 59 145 1.08e-18 80.5
MS.gene21692.t1 AT1G77640 49.474 95 43 2 14 105 38 130 1.36e-18 80.1
MS.gene21692.t1 AT2G35700 37.500 128 69 4 14 135 40 162 6.58e-18 77.0
MS.gene21692.t1 AT2G44940 43.878 98 50 2 10 107 91 183 7.11e-18 79.0
MS.gene21692.t1 AT1G44830 53.409 88 36 2 17 100 33 119 3.40e-17 75.9
MS.gene21692.t1 AT1G22190 50.000 72 34 1 8 79 72 141 6.01e-17 75.9
MS.gene21692.t1 AT2G22200 50.000 74 33 2 7 80 61 130 8.18e-17 75.5
MS.gene21692.t1 AT4G39780 52.381 63 28 1 17 79 91 151 9.67e-17 75.5
MS.gene21692.t1 AT3G16280 48.750 80 36 2 19 98 61 135 1.13e-16 74.7
MS.gene21692.t1 AT3G16280 48.750 80 36 2 19 98 79 153 1.40e-16 74.7
MS.gene21692.t1 AT5G25390 45.455 88 44 2 15 100 3 88 5.30e-16 72.0
MS.gene21692.t1 AT4G27950 38.679 106 60 3 15 120 114 214 5.33e-16 74.3
MS.gene21692.t1 AT2G25820 55.738 61 25 1 19 79 26 84 5.87e-16 72.8
MS.gene21692.t1 AT4G13620 50.794 63 29 1 17 79 230 290 1.19e-15 73.6
MS.gene21692.t1 AT1G33760 46.739 92 44 2 15 106 16 102 1.68e-15 70.5
MS.gene21692.t1 AT2G36450 50.649 77 33 3 9 82 3 77 1.76e-15 70.5
MS.gene21692.t1 AT1G75490 52.113 71 32 1 13 83 36 104 1.78e-15 70.9
MS.gene21692.t1 AT5G61890 43.023 86 42 2 17 97 88 171 2.38e-15 71.2
MS.gene21692.t1 AT5G65130 41.860 86 45 2 17 102 109 189 3.21e-15 71.6
MS.gene21692.t1 AT1G78080 52.381 63 28 1 17 79 150 210 3.47e-15 72.0
MS.gene21692.t1 AT5G65130 41.860 86 45 2 17 102 111 191 3.66e-15 71.2
MS.gene21692.t1 AT3G11020 50.000 70 33 1 13 82 72 139 3.94e-15 71.6
MS.gene21692.t1 AT5G13330 50.746 67 31 1 12 78 32 96 5.44e-15 69.7
MS.gene21692.t1 AT1G36060 52.381 63 28 1 17 79 141 201 5.78e-15 71.2
MS.gene21692.t1 AT2G44840 51.515 66 29 2 18 82 91 154 7.45e-15 69.7
MS.gene21692.t1 AT1G22985 46.053 76 38 1 15 87 71 146 1.40e-14 67.8
MS.gene21692.t1 AT5G05410 30.636 173 96 6 13 164 73 242 1.78e-14 69.7
MS.gene21692.t1 AT2G20880 50.000 64 30 1 17 80 185 246 2.05e-14 69.7
MS.gene21692.t1 AT4G28140 53.226 62 27 1 17 78 141 200 2.85e-14 68.9
MS.gene21692.t1 AT5G05410 30.636 173 96 6 13 164 73 242 3.38e-14 69.3
MS.gene21692.t1 AT3G61630 41.489 94 44 2 17 99 103 196 5.53e-14 68.6
MS.gene21692.t1 AT5G51990 43.182 88 45 2 19 106 54 136 6.18e-14 67.4
MS.gene21692.t1 AT4G11140 50.820 61 28 1 17 77 85 143 6.37e-14 68.2
MS.gene21692.t1 AT4G23750 50.000 64 32 0 8 71 111 174 8.17e-14 68.2
MS.gene21692.t1 AT4G23750 50.000 64 32 0 8 71 111 174 8.17e-14 68.2
MS.gene21692.t1 AT1G77200 51.190 84 36 3 15 98 39 117 1.06e-13 67.0
MS.gene21692.t1 AT5G53290 54.545 55 25 0 17 71 123 177 1.10e-13 67.8
MS.gene21692.t1 AT2G33710 41.892 74 41 1 7 80 58 129 1.45e-13 66.2
MS.gene21692.t1 AT2G33710 41.892 74 41 1 7 80 58 129 1.61e-13 66.2
MS.gene21692.t1 AT1G12610 36.893 103 60 2 19 121 30 127 2.50e-13 65.5
MS.gene21692.t1 AT5G25390 44.318 88 42 3 15 100 3 85 3.71e-13 64.3
MS.gene21692.t1 AT1G15360 50.820 61 28 1 17 77 5 63 3.95e-13 64.7
MS.gene21692.t1 AT1G15360 50.820 61 28 1 17 77 5 63 3.95e-13 64.7
MS.gene21692.t1 AT2G40340 49.180 61 29 1 19 79 14 72 5.59e-13 65.5
MS.gene21692.t1 AT2G40340 49.180 61 29 1 19 79 14 72 5.59e-13 65.5
MS.gene21692.t1 AT2G40340 49.180 61 29 1 19 79 14 72 5.59e-13 65.5
MS.gene21692.t1 AT3G23230 50.000 66 30 2 13 77 14 77 6.40e-13 62.8
MS.gene21692.t1 AT5G13910 47.692 65 32 1 12 76 13 75 8.48e-13 63.9
MS.gene21692.t1 AT5G25190 49.180 61 29 1 17 77 6 64 8.52e-13 63.5
MS.gene21692.t1 AT4G25470 38.542 96 42 3 19 108 51 135 1.16e-12 63.5
MS.gene21692.t1 AT4G25480 53.448 58 25 1 19 76 51 106 1.25e-12 63.5
MS.gene21692.t1 AT2G40340 49.180 61 29 1 19 79 72 130 1.28e-12 64.3
MS.gene21692.t1 AT2G40340 49.180 61 29 1 19 79 72 130 1.32e-12 64.7
MS.gene21692.t1 AT1G12890 61.364 44 17 0 18 61 20 63 1.41e-12 63.5
MS.gene21692.t1 AT2G40340 49.180 61 29 1 19 79 79 137 1.42e-12 64.7
MS.gene21692.t1 AT3G16770 47.541 61 30 1 19 79 79 137 1.67e-12 63.5
MS.gene21692.t1 AT2G47520 42.667 75 36 2 8 77 34 106 1.67e-12 62.4
MS.gene21692.t1 AT3G57600 50.000 62 29 1 18 79 27 86 2.23e-12 63.5
MS.gene21692.t1 AT3G15210 39.759 83 45 2 1 80 4 84 2.49e-12 62.8
MS.gene21692.t1 AT1G63030 32.759 116 73 2 13 128 24 134 2.75e-12 62.0
MS.gene21692.t1 AT1G63030 32.759 116 73 2 13 128 24 134 2.75e-12 62.0
MS.gene21692.t1 AT4G25490 53.448 58 25 1 19 76 48 103 2.83e-12 62.4
MS.gene21692.t1 AT1G28360 47.619 63 31 1 19 81 12 72 3.62e-12 62.0
MS.gene21692.t1 AT1G71130 44.928 69 33 1 14 77 74 142 4.61e-12 61.2
MS.gene21692.t1 AT2G46310 56.818 44 19 0 18 61 98 141 8.03e-12 62.4
MS.gene21692.t1 AT1G72360 33.019 106 63 2 4 109 10 107 8.17e-12 61.2
MS.gene21692.t1 AT1G72360 33.019 106 63 2 4 109 59 156 9.76e-12 61.6
MS.gene21692.t1 AT1G72360 33.333 105 62 2 5 109 62 158 1.34e-11 61.2
MS.gene21692.t1 AT5G43410 46.875 64 31 2 16 78 12 73 1.35e-11 59.3
MS.gene21692.t1 AT1G28160 59.091 44 18 0 18 61 38 81 1.80e-11 60.8
MS.gene21692.t1 AT5G11190 42.222 90 46 2 15 100 3 90 1.81e-11 60.1
MS.gene21692.t1 AT5G44210 47.458 59 29 1 19 77 33 89 1.95e-11 60.1
MS.gene21692.t1 AT1G28370 46.939 49 26 0 11 59 12 60 2.66e-11 59.3
MS.gene21692.t1 AT1G28370 46.939 49 26 0 11 59 50 98 2.79e-11 59.7
MS.gene21692.t1 AT1G12980 36.986 73 46 0 5 77 43 115 3.96e-11 60.5
MS.gene21692.t1 AT5G47220 46.774 62 30 2 17 77 115 174 4.15e-11 59.7
MS.gene21692.t1 AT5G18560 47.458 59 29 1 18 76 54 110 4.30e-11 60.5
MS.gene21692.t1 AT2G40350 44.615 65 34 1 13 77 68 130 6.75e-11 58.2
MS.gene21692.t1 AT2G40350 44.615 65 34 1 13 77 61 123 8.00e-11 57.8
MS.gene21692.t1 AT5G18450 55.102 49 22 0 13 61 28 76 8.34e-11 59.3
MS.gene21692.t1 AT1G43160 42.105 76 42 1 17 92 59 132 8.53e-11 58.2

Find 29 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGGAACTCAAGAACGTTTA+TGG 0.132390 5.2:+2610516 MS.gene21692:CDS
TTGTCCATATGATTCCTTTC+TGG 0.191604 5.2:-2610767 None:intergenic
TAACAACAACAAGAAGTTTA+AGG 0.235066 5.2:+2610445 MS.gene21692:CDS
TGTGCCACTGCAGCAGCTTC+TGG 0.288160 5.2:-2610557 None:intergenic
TTTAAAGAGACCTTCTACTT+TGG 0.299862 5.2:+2610598 MS.gene21692:CDS
ATTACACATTGCAGATGAAT+TGG 0.357936 5.2:+2610393 None:intergenic
GGTATCAGAGATTCGTGTTC+CGG 0.375282 5.2:+2610496 MS.gene21692:CDS
TGTTCAAATCCTCTTGTTGT+TGG 0.378910 5.2:-2610859 None:intergenic
ACAAGAAGTTTAAGGGAGTT+AGG 0.389298 5.2:+2610453 MS.gene21692:CDS
GTATCAGAGATTCGTGTTCC+GGG 0.419275 5.2:+2610497 MS.gene21692:CDS
AACAACAACAAGAAGTTTAA+GGG 0.428175 5.2:+2610446 MS.gene21692:CDS
TTGAGTTTGTCCAAAGTAGA+AGG 0.430828 5.2:-2610608 None:intergenic
AGGGAGTTAGGCGTAGAAAG+TGG 0.439329 5.2:+2610465 MS.gene21692:CDS
GTCTCCTAGGTCTGTTCAAA+AGG 0.440851 5.2:+2610682 MS.gene21692:CDS
CATAAACGTTCTTGAGTTCC+CGG 0.450969 5.2:-2610515 None:intergenic
GGGAGTTAGGCGTAGAAAGT+GGG 0.457108 5.2:+2610466 MS.gene21692:CDS
AACTCAAGAACGTTTATGGT+TGG 0.469500 5.2:+2610520 MS.gene21692:CDS
GAGATAATTTGATGTCTCCT+AGG 0.471023 5.2:+2610669 MS.gene21692:CDS
TTTGCCAGAAAGGAATCATA+TGG 0.472058 5.2:+2610763 MS.gene21692:CDS
AAGCAACAACTTTGCCAGAA+AGG 0.482224 5.2:+2610753 MS.gene21692:CDS
ACTCAAGAACGTTTATGGTT+GGG 0.482883 5.2:+2610521 MS.gene21692:CDS
GGAGTTAGGCGTAGAAAGTG+GGG 0.510663 5.2:+2610467 MS.gene21692:CDS
AAATTATAGATAATCTTCAA+TGG 0.511121 5.2:-2610885 None:intergenic
CAATAAGAAGACAAAATCTC+AGG 0.516423 5.2:-2610635 None:intergenic
GAAGGTTTGGGTGATGATCA+AGG 0.545874 5.2:+2610818 MS.gene21692:CDS
AGTTTCTAGCCAACAACAAG+AGG 0.563778 5.2:+2610850 MS.gene21692:CDS
GGTTGCTTCAGATGTTGCTA+TGG 0.573939 5.2:+2610703 MS.gene21692:CDS
TACACCAGAAGCTGCTGCAG+TGG 0.594266 5.2:+2610553 MS.gene21692:CDS
GAGTTAGGCGTAGAAAGTGG+GGG 0.654241 5.2:+2610468 MS.gene21692:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AACAACAACAAGAAGTTTAA+GGG + chr5.2:2610446-2610465 MS.gene21692:CDS 25.0%
! TAACAACAACAAGAAGTTTA+AGG + chr5.2:2610445-2610464 MS.gene21692:CDS 25.0%
CAATAAGAAGACAAAATCTC+AGG - chr5.2:2610638-2610657 None:intergenic 30.0%
TTTAAAGAGACCTTCTACTT+TGG + chr5.2:2610598-2610617 MS.gene21692:CDS 30.0%
!!! TTGTTGCTGATGATGTTTTT+TGG + chr5.2:2610792-2610811 MS.gene21692:CDS 30.0%
AACTCAAGAACGTTTATGGT+TGG + chr5.2:2610520-2610539 MS.gene21692:CDS 35.0%
ACAAGAAGTTTAAGGGAGTT+AGG + chr5.2:2610453-2610472 MS.gene21692:CDS 35.0%
ACTCAAGAACGTTTATGGTT+GGG + chr5.2:2610521-2610540 MS.gene21692:CDS 35.0%
GAGATAATTTGATGTCTCCT+AGG + chr5.2:2610669-2610688 MS.gene21692:CDS 35.0%
TTGAGTTTGTCCAAAGTAGA+AGG - chr5.2:2610611-2610630 None:intergenic 35.0%
TTGTCCATATGATTCCTTTC+TGG - chr5.2:2610770-2610789 None:intergenic 35.0%
TTTGCCAGAAAGGAATCATA+TGG + chr5.2:2610763-2610782 MS.gene21692:CDS 35.0%
! TGTTCAAATCCTCTTGTTGT+TGG - chr5.2:2610862-2610881 None:intergenic 35.0%
!!! TGCTGATGATGTTTTTTGGT+GGG + chr5.2:2610796-2610815 MS.gene21692:CDS 35.0%
!!! TTGCTGATGATGTTTTTTGG+TGG + chr5.2:2610795-2610814 MS.gene21692:CDS 35.0%
AAGCAACAACTTTGCCAGAA+AGG + chr5.2:2610753-2610772 MS.gene21692:CDS 40.0%
CATAAACGTTCTTGAGTTCC+CGG - chr5.2:2610518-2610537 None:intergenic 40.0%
!! AGTTTCTAGCCAACAACAAG+AGG + chr5.2:2610850-2610869 MS.gene21692:CDS 40.0%
!!! GATGATGTTTTTTGGTGGGA+AGG + chr5.2:2610800-2610819 MS.gene21692:CDS 40.0%
!!! GTTTTTTGGTGGGAAGGTTT+GGG + chr5.2:2610806-2610825 MS.gene21692:CDS 40.0%
!!! TGTTTTTTGGTGGGAAGGTT+TGG + chr5.2:2610805-2610824 MS.gene21692:CDS 40.0%
CGGGAACTCAAGAACGTTTA+TGG + chr5.2:2610516-2610535 MS.gene21692:CDS 45.0%
GAAGGTTTGGGTGATGATCA+AGG + chr5.2:2610818-2610837 MS.gene21692:CDS 45.0%
GGTATCAGAGATTCGTGTTC+CGG + chr5.2:2610496-2610515 MS.gene21692:CDS 45.0%
GGTTGCTTCAGATGTTGCTA+TGG + chr5.2:2610703-2610722 MS.gene21692:CDS 45.0%
GTATCAGAGATTCGTGTTCC+GGG + chr5.2:2610497-2610516 MS.gene21692:CDS 45.0%
GTCTCCTAGGTCTGTTCAAA+AGG + chr5.2:2610682-2610701 MS.gene21692:CDS 45.0%
! GCAACCTTTTGAACAGACCT+AGG - chr5.2:2610689-2610708 None:intergenic 45.0%
AGGGAGTTAGGCGTAGAAAG+TGG + chr5.2:2610465-2610484 MS.gene21692:CDS 50.0%
GAGTTAGGCGTAGAAAGTGG+GGG + chr5.2:2610468-2610487 MS.gene21692:CDS 50.0%
GCGTAGAAAGTGGGGGAAAT+GGG + chr5.2:2610475-2610494 MS.gene21692:CDS 50.0%
GGAGTTAGGCGTAGAAAGTG+GGG + chr5.2:2610467-2610486 MS.gene21692:CDS 50.0%
GGGAGTTAGGCGTAGAAAGT+GGG + chr5.2:2610466-2610485 MS.gene21692:CDS 50.0%
GGCGTAGAAAGTGGGGGAAA+TGG + chr5.2:2610474-2610493 MS.gene21692:CDS 55.0%
TACACCAGAAGCTGCTGCAG+TGG + chr5.2:2610553-2610572 MS.gene21692:CDS 55.0%
! TGTGCCACTGCAGCAGCTTC+TGG - chr5.2:2610560-2610579 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 2610407 2610904 2610407 ID=MS.gene21692
chr5.2 mRNA 2610407 2610904 2610407 ID=MS.gene21692.t1;Parent=MS.gene21692
chr5.2 exon 2610407 2610904 2610407 ID=MS.gene21692.t1.exon1;Parent=MS.gene21692.t1
chr5.2 CDS 2610407 2610904 2610407 ID=cds.MS.gene21692.t1;Parent=MS.gene21692.t1
Gene Sequence

>MS.gene21692

ATGAATTGGTATACTTCAACAACAAGCACAAGTGATGATAACAACAACAAGAAGTTTAAGGGAGTTAGGCGTAGAAAGTGGGGGAAATGGGTATCAGAGATTCGTGTTCCGGGAACTCAAGAACGTTTATGGTTGGGAACTTATGCTACACCAGAAGCTGCTGCAGTGGCACACGATGTAGCTTTTTATTGTTTAAAGAGACCTTCTACTTTGGACAAACTCAACTTTCCTGAGATTTTGTCTTCTTATTGTATTCAACAAAGAGATAATTTGATGTCTCCTAGGTCTGTTCAAAAGGTTGCTTCAGATGTTGCTATGGATGTTGATGCACGAAACATTGCAAGTCAAGCAACAACTTTGCCAGAAAGGAATCATATGGACAATGTTGTTGCTGATGATGTTTTTTGGTGGGAAGGTTTGGGTGATGATCAAGGAGTTACTCAAGTTTCTAGCCAACAACAAGAGGATTTGAACATTTCCATTGAAGATTATCTATAA
Protein sequence

>MS.gene21692.t1

MNWYTSTTSTSDDNNNKKFKGVRRRKWGKWVSEIRVPGTQERLWLGTYATPEAAAVAHDVAFYCLKRPSTLDKLNFPEILSSYCIQQRDNLMSPRSVQKVASDVAMDVDARNIASQATTLPERNHMDNVVADDVFWWEGLGDDQGVTQVSSQQQEDLNISIEDYL