Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46264.t1 | XP_003602050.1 | 98.3 | 58 | 1 | 0 | 1 | 58 | 1 | 58 | 1.20E-22 | 116.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46264.t1 | G7LGL0 | 98.3 | 58 | 1 | 0 | 1 | 58 | 1 | 58 | 9.0e-23 | 116.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene46264 | MS.gene09779 | PPI |
| MS.gene46264 | MS.gene65180 | PPI |
| MS.gene46264 | MS.gene025830 | PPI |
| MS.gene46264 | MS.gene21936 | PPI |
| MS.gene46264 | MS.gene034249 | PPI |
| MS.gene064420 | MS.gene46264 | PPI |
| MS.gene55165 | MS.gene46264 | PPI |
| MS.gene46264 | MS.gene015411 | PPI |
| MS.gene024713 | MS.gene46264 | PPI |
| MS.gene46264 | MS.gene70215 | PPI |
| MS.gene02807 | MS.gene46264 | PPI |
| MS.gene80684 | MS.gene46264 | PPI |
| MS.gene57391 | MS.gene46264 | PPI |
| MS.gene46264 | MS.gene000610 | PPI |
| MS.gene09780 | MS.gene46264 | PPI |
| MS.gene030614 | MS.gene46264 | PPI |
| MS.gene46264 | MS.gene053224 | PPI |
| MS.gene46264 | MS.gene28384 | PPI |
| MS.gene09762 | MS.gene46264 | PPI |
| MS.gene21692 | MS.gene46264 | PPI |
| MS.gene80813 | MS.gene46264 | PPI |
| MS.gene46264 | MS.gene064850 | PPI |
| MS.gene46264 | MS.gene070008 | PPI |
| MS.gene46264 | MS.gene014333 | PPI |
| MS.gene29628 | MS.gene46264 | PPI |
| MS.gene46264 | MS.gene69319 | PPI |
| MS.gene22276 | MS.gene46264 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46264.t1 | MTR_3g088410 | 98.701 | 154 | 2 | 0 | 1 | 154 | 1 | 154 | 5.88e-110 | 309 |
| MS.gene46264.t1 | MTR_8g056020 | 37.984 | 129 | 80 | 0 | 19 | 147 | 228 | 356 | 5.76e-17 | 76.6 |
| MS.gene46264.t1 | MTR_5g026800 | 34.127 | 126 | 82 | 1 | 24 | 148 | 15 | 140 | 2.96e-15 | 70.9 |
| MS.gene46264.t1 | MTR_5g043640 | 40.000 | 90 | 54 | 0 | 38 | 127 | 108 | 197 | 2.28e-13 | 65.5 |
| MS.gene46264.t1 | MTR_3g491870 | 33.696 | 92 | 61 | 0 | 21 | 112 | 238 | 329 | 1.35e-12 | 64.3 |
| MS.gene46264.t1 | MTR_1g089180 | 37.755 | 98 | 61 | 0 | 22 | 119 | 342 | 439 | 1.45e-12 | 64.3 |
| MS.gene46264.t1 | MTR_1g086410 | 60.000 | 45 | 18 | 0 | 47 | 91 | 11 | 55 | 7.56e-12 | 57.4 |
| MS.gene46264.t1 | MTR_6g027840 | 34.831 | 89 | 58 | 0 | 24 | 112 | 368 | 456 | 2.50e-11 | 60.8 |
| MS.gene46264.t1 | MTR_3g107720 | 33.333 | 114 | 74 | 1 | 27 | 140 | 543 | 654 | 7.31e-11 | 59.7 |
| MS.gene46264.t1 | MTR_3g009280 | 32.174 | 115 | 75 | 1 | 13 | 124 | 214 | 328 | 9.26e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46264.t1 | AT5G12320 | 64.179 | 134 | 48 | 0 | 14 | 147 | 5 | 138 | 5.84e-60 | 182 |
| MS.gene46264.t1 | AT2G03430 | 34.921 | 126 | 78 | 1 | 19 | 140 | 45 | 170 | 7.72e-17 | 75.1 |
| MS.gene46264.t1 | AT2G03430 | 35.955 | 89 | 57 | 0 | 39 | 127 | 102 | 190 | 6.96e-11 | 58.9 |
| MS.gene46264.t1 | AT4G27780 | 32.381 | 105 | 71 | 0 | 20 | 124 | 233 | 337 | 3.61e-15 | 71.6 |
| MS.gene46264.t1 | AT4G35450 | 36.364 | 110 | 70 | 0 | 26 | 135 | 186 | 295 | 3.87e-15 | 71.2 |
| MS.gene46264.t1 | AT4G35450 | 36.364 | 110 | 70 | 0 | 26 | 135 | 224 | 333 | 5.83e-15 | 70.9 |
| MS.gene46264.t1 | AT4G35450 | 36.364 | 110 | 70 | 0 | 26 | 135 | 224 | 333 | 5.83e-15 | 70.9 |
| MS.gene46264.t1 | AT4G35450 | 36.364 | 110 | 70 | 0 | 26 | 135 | 224 | 333 | 5.83e-15 | 70.9 |
| MS.gene46264.t1 | AT4G35450 | 36.364 | 110 | 70 | 0 | 26 | 135 | 232 | 341 | 6.55e-15 | 70.9 |
| MS.gene46264.t1 | AT5G53470 | 31.429 | 105 | 72 | 0 | 20 | 124 | 218 | 322 | 1.10e-14 | 70.1 |
| MS.gene46264.t1 | AT4G19150 | 35.398 | 113 | 72 | 1 | 24 | 135 | 20 | 132 | 1.20e-14 | 68.9 |
| MS.gene46264.t1 | AT2G14255 | 33.036 | 112 | 72 | 2 | 6 | 116 | 12 | 121 | 6.00e-14 | 68.6 |
| MS.gene46264.t1 | AT3G09890 | 33.333 | 126 | 72 | 3 | 6 | 122 | 60 | 182 | 2.58e-12 | 62.4 |
| MS.gene46264.t1 | AT2G17390 | 32.727 | 110 | 74 | 0 | 26 | 135 | 226 | 335 | 6.14e-12 | 62.4 |
| MS.gene46264.t1 | AT4G19150 | 35.484 | 93 | 55 | 2 | 50 | 142 | 59 | 146 | 8.85e-11 | 58.2 |
Find 37 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCATTGGGCGTGCCTCAA+TGG | 0.274476 | 3.1:-71278157 | MS.gene46264:CDS |
| TGGTGCTCTGGTTTCTTCAT+AGG | 0.336548 | 3.1:-71277981 | None:intergenic |
| TTCATTGGGCGTGCCTCAAT+GGG | 0.368210 | 3.1:-71278156 | MS.gene46264:CDS |
| GTGAAGAAATTAATCATTGC+TGG | 0.368237 | 3.1:-71278121 | MS.gene46264:CDS |
| AAAAGAATACACCTCTTCAT+TGG | 0.393239 | 3.1:-71278171 | MS.gene46264:CDS |
| CTTCATATGGCTGCAGCAAA+TGG | 0.395615 | 3.1:-71278256 | MS.gene46264:CDS |
| TGATCATCCTTGGAATTAAG+AGG | 0.420728 | 3.1:+71278292 | None:intergenic |
| AGTTCTTCCTTGATCATCCT+TGG | 0.441829 | 3.1:+71278282 | None:intergenic |
| TTCTCTGTCATCGTCTCAGT+TGG | 0.444663 | 3.1:+71278388 | None:intergenic |
| TCATCGTCTCAGTTGGGTCC+TGG | 0.448050 | 3.1:+71278395 | None:intergenic |
| AGTTGAACTTCGTGGTGCTC+TGG | 0.450551 | 3.1:-71277993 | MS.gene46264:CDS |
| AATGGATGAAGCTGTGACTA+GGG | 0.455886 | 3.1:-71278059 | MS.gene46264:CDS |
| AGGAAGAACTGCTCTTCATA+TGG | 0.461895 | 3.1:-71278269 | MS.gene46264:CDS |
| CAATGGATGAAGCTGTGACT+AGG | 0.469105 | 3.1:-71278060 | MS.gene46264:CDS |
| AAAGAATACACCTCTTCATT+GGG | 0.493944 | 3.1:-71278170 | MS.gene46264:CDS |
| CTAGTCACAGCTTCATCCAT+TGG | 0.498266 | 3.1:+71278061 | None:intergenic |
| GTGGAGTATTTAATCAGTAA+AGG | 0.499037 | 3.1:-71278220 | MS.gene46264:CDS |
| CTTAATTCCAAGGATGATCA+AGG | 0.510692 | 3.1:-71278289 | MS.gene46264:CDS |
| TGAAGATAACATCAATATCC+AGG | 0.516924 | 3.1:-71278413 | MS.gene46264:CDS |
| TGGTGTTCCTCTTAATTCCA+AGG | 0.535842 | 3.1:-71278299 | MS.gene46264:CDS |
| TCTCTGTCATCGTCTCAGTT+GGG | 0.542518 | 3.1:+71278389 | None:intergenic |
| TAGGGGCAAACTTGAAGTGA+TGG | 0.585162 | 3.1:-71278041 | MS.gene46264:CDS |
| GGAATTAAGAGGAACACCAT+TGG | 0.586019 | 3.1:+71278303 | None:intergenic |
| GAGGCACTAGTTGAACTTCG+TGG | 0.586185 | 3.1:-71278001 | MS.gene46264:CDS |
| TTGAGGCACGCCCAATGAAG+AGG | 0.592105 | 3.1:+71278160 | None:intergenic |
| GAATATTGAAGCCTTGCTTG+AGG | 0.596172 | 3.1:-71278368 | MS.gene46264:CDS |
| AAATGGACATATTAATATTG+TGG | 0.599091 | 3.1:-71278239 | MS.gene46264:CDS |
| CAACCTCAACATGCCCATTG+AGG | 0.600476 | 3.1:+71278143 | None:intergenic |
| CAGTTATGAGAGGACTCCAA+TGG | 0.604606 | 3.1:-71278077 | MS.gene46264:CDS |
| ATATCTTGCTGCCTCAAGCA+AGG | 0.614678 | 3.1:+71278357 | None:intergenic |
| GTGCCTCAATGGGCATGTTG+AGG | 0.622233 | 3.1:-71278146 | MS.gene46264:CDS |
| GGATCTTAATTCAGAAAATG+TGG | 0.626131 | 3.1:-71278194 | MS.gene46264:CDS |
| GGATGCAATTAATGAAGCAG+AGG | 0.634709 | 3.1:-71278020 | MS.gene46264:CDS |
| ATGGATGAAGCTGTGACTAG+GGG | 0.659988 | 3.1:-71278058 | MS.gene46264:CDS |
| GTATTTAATCAGTAAAGGAG+CGG | 0.669015 | 3.1:-71278215 | MS.gene46264:CDS |
| GTTGTGAGCTTAACATCCAA+TGG | 0.676653 | 3.1:-71278319 | MS.gene46264:CDS |
| GGCAGCAAGATATGATGACA+TGG | 0.679883 | 3.1:-71278347 | MS.gene46264:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATGGACATATTAATATTG+TGG | - | chr3.1:71278167-71278186 | MS.gene46264:CDS | 20.0% |
| AAAAGAATACACCTCTTCAT+TGG | - | chr3.1:71278235-71278254 | MS.gene46264:CDS | 30.0% | |
| AAAGAATACACCTCTTCATT+GGG | - | chr3.1:71278236-71278255 | MS.gene46264:CDS | 30.0% | |
| GGATCTTAATTCAGAAAATG+TGG | - | chr3.1:71278212-71278231 | MS.gene46264:CDS | 30.0% | |
| GTATTTAATCAGTAAAGGAG+CGG | - | chr3.1:71278191-71278210 | MS.gene46264:CDS | 30.0% | |
| GTGAAGAAATTAATCATTGC+TGG | - | chr3.1:71278285-71278304 | MS.gene46264:CDS | 30.0% | |
| TGAAGATAACATCAATATCC+AGG | - | chr3.1:71277993-71278012 | MS.gene46264:CDS | 30.0% | |
| ! | GTGGAGTATTTAATCAGTAA+AGG | - | chr3.1:71278186-71278205 | MS.gene46264:CDS | 30.0% |
| !! | GTGTTTTAAACAGTTATGAG+AGG | - | chr3.1:71278319-71278338 | MS.gene46264:CDS | 30.0% |
| CTTAATTCCAAGGATGATCA+AGG | - | chr3.1:71278117-71278136 | MS.gene46264:CDS | 35.0% | |
| TGATCATCCTTGGAATTAAG+AGG | + | chr3.1:71278117-71278136 | None:intergenic | 35.0% | |
| AATGGATGAAGCTGTGACTA+GGG | - | chr3.1:71278347-71278366 | MS.gene46264:CDS | 40.0% | |
| AGGAAGAACTGCTCTTCATA+TGG | - | chr3.1:71278137-71278156 | MS.gene46264:CDS | 40.0% | |
| AGTTCTTCCTTGATCATCCT+TGG | + | chr3.1:71278127-71278146 | None:intergenic | 40.0% | |
| GAATATTGAAGCCTTGCTTG+AGG | - | chr3.1:71278038-71278057 | MS.gene46264:CDS | 40.0% | |
| GGAATTAAGAGGAACACCAT+TGG | + | chr3.1:71278106-71278125 | None:intergenic | 40.0% | |
| GGATGCAATTAATGAAGCAG+AGG | - | chr3.1:71278386-71278405 | MS.gene46264:CDS | 40.0% | |
| GTTGTGAGCTTAACATCCAA+TGG | - | chr3.1:71278087-71278106 | MS.gene46264:CDS | 40.0% | |
| TGGTGTTCCTCTTAATTCCA+AGG | - | chr3.1:71278107-71278126 | MS.gene46264:CDS | 40.0% | |
| ATATCTTGCTGCCTCAAGCA+AGG | + | chr3.1:71278052-71278071 | None:intergenic | 45.0% | |
| ATGGATGAAGCTGTGACTAG+GGG | - | chr3.1:71278348-71278367 | MS.gene46264:CDS | 45.0% | |
| CAATGGATGAAGCTGTGACT+AGG | - | chr3.1:71278346-71278365 | MS.gene46264:CDS | 45.0% | |
| CAGTTATGAGAGGACTCCAA+TGG | - | chr3.1:71278329-71278348 | MS.gene46264:CDS | 45.0% | |
| CTAGTCACAGCTTCATCCAT+TGG | + | chr3.1:71278348-71278367 | None:intergenic | 45.0% | |
| CTTCATATGGCTGCAGCAAA+TGG | - | chr3.1:71278150-71278169 | MS.gene46264:CDS | 45.0% | |
| GGCAGCAAGATATGATGACA+TGG | - | chr3.1:71278059-71278078 | MS.gene46264:CDS | 45.0% | |
| TAGGGGCAAACTTGAAGTGA+TGG | - | chr3.1:71278365-71278384 | MS.gene46264:CDS | 45.0% | |
| TCTCTGTCATCGTCTCAGTT+GGG | + | chr3.1:71278020-71278039 | None:intergenic | 45.0% | |
| TTCTCTGTCATCGTCTCAGT+TGG | + | chr3.1:71278021-71278040 | None:intergenic | 45.0% | |
| AGTTGAACTTCGTGGTGCTC+TGG | - | chr3.1:71278413-71278432 | MS.gene46264:CDS | 50.0% | |
| CAACCTCAACATGCCCATTG+AGG | + | chr3.1:71278266-71278285 | None:intergenic | 50.0% | |
| TTCATTGGGCGTGCCTCAAT+GGG | - | chr3.1:71278250-71278269 | MS.gene46264:CDS | 50.0% | |
| ! | GAGGCACTAGTTGAACTTCG+TGG | - | chr3.1:71278405-71278424 | MS.gene46264:CDS | 50.0% |
| CTTCATTGGGCGTGCCTCAA+TGG | - | chr3.1:71278249-71278268 | MS.gene46264:CDS | 55.0% | |
| GTGCCTCAATGGGCATGTTG+AGG | - | chr3.1:71278260-71278279 | MS.gene46264:CDS | 55.0% | |
| TCATCGTCTCAGTTGGGTCC+TGG | + | chr3.1:71278014-71278033 | None:intergenic | 55.0% | |
| TTGAGGCACGCCCAATGAAG+AGG | + | chr3.1:71278249-71278268 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 71277982 | 71278446 | 71277982 | ID=MS.gene46264 |
| chr3.1 | mRNA | 71277982 | 71278446 | 71277982 | ID=MS.gene46264.t1;Parent=MS.gene46264 |
| chr3.1 | exon | 71277982 | 71278446 | 71277982 | ID=MS.gene46264.t1.exon1;Parent=MS.gene46264.t1 |
| chr3.1 | CDS | 71277982 | 71278446 | 71277982 | ID=cds.MS.gene46264.t1;Parent=MS.gene46264.t1 |
| Gene Sequence |
| Protein sequence |
