AlfalfaGEDB | MS.gene02572

M. sativa cultivar XinJiangDaYe / MS.gene02572

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF13857.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02572.t1	XP_003602050.1	96.6	58	2	0	1	58	1	58	3.60E-22	114.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02572.t1	G7LGL0	96.6	58	2	0	1	58	1	58	2.6e-22	114.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene22276	MS.gene02572	PPI
MS.gene02572	MS.gene025830	PPI
MS.gene28384	MS.gene02572	PPI
MS.gene02572	MS.gene21936	PPI
MS.gene02572	MS.gene034249	PPI
MS.gene064420	MS.gene02572	PPI
MS.gene02807	MS.gene02572	PPI
MS.gene57391	MS.gene02572	PPI
MS.gene09780	MS.gene02572	PPI
MS.gene024713	MS.gene02572	PPI
MS.gene09762	MS.gene02572	PPI
MS.gene80684	MS.gene02572	PPI
MS.gene80813	MS.gene02572	PPI
MS.gene02572	MS.gene053224	PPI
MS.gene015411	MS.gene02572	PPI
MS.gene030614	MS.gene02572	PPI
MS.gene21692	MS.gene02572	PPI
MS.gene65180	MS.gene02572	PPI
MS.gene29628	MS.gene02572	PPI
MS.gene02572	MS.gene000610	PPI
MS.gene064850	MS.gene02572	PPI
MS.gene70215	MS.gene02572	PPI
MS.gene02572	MS.gene070008	PPI
MS.gene02572	MS.gene014333	PPI
MS.gene02572	MS.gene69319	PPI
MS.gene55165	MS.gene02572	PPI
MS.gene02572	MS.gene09779	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02572.t1	MTR_3g088410	98.052	154	3	0	1	154	1	154	1.90e-109	308
MS.gene02572.t1	MTR_8g056020	37.209	129	81	0	19	147	228	356	1.50e-16	75.5
MS.gene02572.t1	MTR_5g026800	34.146	123	80	1	27	148	18	140	2.78e-15	71.2
MS.gene02572.t1	MTR_5g043640	40.000	90	54	0	38	127	108	197	2.21e-13	65.5
MS.gene02572.t1	MTR_3g491870	33.696	92	61	0	21	112	238	329	1.41e-12	64.3
MS.gene02572.t1	MTR_1g089180	36.735	98	62	0	22	119	342	439	4.07e-12	63.2
MS.gene02572.t1	MTR_1g086410	60.000	45	18	0	47	91	11	55	7.40e-12	57.4
MS.gene02572.t1	MTR_6g027840	34.831	89	58	0	24	112	368	456	2.27e-11	60.8
MS.gene02572.t1	MTR_3g009280	32.456	114	76	1	12	124	215	328	4.98e-11	59.7
MS.gene02572.t1	MTR_3g107720	33.333	114	74	1	27	140	543	654	7.11e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02572.t1	AT5G12320	63.433	134	49	0	14	147	5	138	1.36e-59	181
MS.gene02572.t1	AT2G03430	38.947	95	58	0	46	140	76	170	8.39e-17	74.7
MS.gene02572.t1	AT2G03430	35.955	89	57	0	39	127	102	190	6.49e-11	58.9
MS.gene02572.t1	AT4G35450	36.364	110	70	0	26	135	186	295	3.95e-15	71.2
MS.gene02572.t1	AT4G27780	32.381	105	71	0	20	124	233	337	4.96e-15	71.2
MS.gene02572.t1	AT4G35450	36.364	110	70	0	26	135	224	333	6.50e-15	70.9
MS.gene02572.t1	AT4G35450	36.364	110	70	0	26	135	224	333	6.50e-15	70.9
MS.gene02572.t1	AT4G35450	36.364	110	70	0	26	135	224	333	6.50e-15	70.9
MS.gene02572.t1	AT4G35450	36.364	110	70	0	26	135	232	341	6.95e-15	70.9
MS.gene02572.t1	AT5G53470	31.429	105	72	0	20	124	218	322	1.33e-14	70.1
MS.gene02572.t1	AT4G19150	35.398	113	72	1	24	135	20	132	1.48e-14	68.9
MS.gene02572.t1	AT2G14255	33.036	112	72	2	6	116	12	121	9.17e-14	67.8
MS.gene02572.t1	AT3G09890	33.333	126	72	3	6	122	60	182	2.50e-12	62.4
MS.gene02572.t1	AT2G17390	32.727	110	74	0	26	135	226	335	5.90e-12	62.4
MS.gene02572.t1	AT4G19150	35.484	93	55	2	50	142	59	146	8.67e-11	58.2

Find 38 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTCATTGGGCGTGCCTCAA+TGG	0.274476	3.2:-75328913	MS.gene02572:CDS
TGGTGCTCTGGTTTCTTCAT+AGG	0.336548	3.2:-75328737	None:intergenic
TTCATTGGGCGTGCCTCAAT+GGG	0.368210	3.2:-75328912	MS.gene02572:CDS
GTGAAGAAATTAATCATTGC+TGG	0.368237	3.2:-75328877	MS.gene02572:CDS
AAAAGAATACACCTCTTCAT+TGG	0.393239	3.2:-75328927	MS.gene02572:CDS
CTTCATATGGCTGCAGCAAA+TGG	0.395615	3.2:-75329012	MS.gene02572:CDS
TGATCATCCTTGGAATTAAG+AGG	0.420728	3.2:+75329048	None:intergenic
GACAGAGAATATTGAAGCCT+TGG	0.429399	3.2:-75329130	MS.gene02572:CDS
AGTTCTTCCTTGATCATCCT+TGG	0.441829	3.2:+75329038	None:intergenic
TTCTCTGTCATCGTCTCAGT+TGG	0.444663	3.2:+75329144	None:intergenic
TCATCGTCTCAGTTGGGTCC+TGG	0.448050	3.2:+75329151	None:intergenic
AGTTGAACTTCGTGGTGCTC+TGG	0.450551	3.2:-75328749	MS.gene02572:CDS
AATGGATGAAGCTGTGACTA+GGG	0.455886	3.2:-75328815	MS.gene02572:CDS
AGGAAGAACTGCTCTTCATA+TGG	0.461895	3.2:-75329025	MS.gene02572:CDS
CAATGGATGAAGCTGTGACT+AGG	0.469105	3.2:-75328816	MS.gene02572:CDS
AAAGAATACACCTCTTCATT+GGG	0.493944	3.2:-75328926	MS.gene02572:CDS
CTAGTCACAGCTTCATCCAT+TGG	0.498266	3.2:+75328817	None:intergenic
GTGGAGTATTTAATCAGTAA+AGG	0.499037	3.2:-75328976	MS.gene02572:CDS
CGAAGATAACATCAATATCC+AGG	0.509750	3.2:-75329169	MS.gene02572:CDS
CTTAATTCCAAGGATGATCA+AGG	0.510692	3.2:-75329045	MS.gene02572:CDS
GAATATTGAAGCCTTGGTTG+AGG	0.517668	3.2:-75329124	MS.gene02572:CDS
TGGTGTTCCTCTTAATTCCA+AGG	0.535842	3.2:-75329055	MS.gene02572:CDS
TCTCTGTCATCGTCTCAGTT+GGG	0.542518	3.2:+75329145	None:intergenic
TAGGGGCAAACTTGAAGTGA+TGG	0.585162	3.2:-75328797	MS.gene02572:CDS
GGAATTAAGAGGAACACCAT+TGG	0.586019	3.2:+75329059	None:intergenic
GAGGCACTAGTTGAACTTCG+TGG	0.586185	3.2:-75328757	MS.gene02572:CDS
TTGAGGCACGCCCAATGAAG+AGG	0.592105	3.2:+75328916	None:intergenic
AAATGGACATATTAATATTG+TGG	0.599091	3.2:-75328995	MS.gene02572:CDS
CAACCTCAACATGCCCATTG+AGG	0.600476	3.2:+75328899	None:intergenic
CAGTTATGAGAGGACTCCAA+TGG	0.604606	3.2:-75328833	MS.gene02572:CDS
GTGCCTCAATGGGCATGTTG+AGG	0.622233	3.2:-75328902	MS.gene02572:CDS
GGATCTTAATTCAGAAAATG+TGG	0.626131	3.2:-75328950	MS.gene02572:CDS
GGATGCAATTAATGAAGCAG+AGG	0.634709	3.2:-75328776	MS.gene02572:CDS
ATATCTTGCTGCCTCAACCA+AGG	0.648617	3.2:+75329113	None:intergenic
ATGGATGAAGCTGTGACTAG+GGG	0.659988	3.2:-75328814	MS.gene02572:CDS
GTATTTAATCAGTAAAGGAG+CGG	0.669015	3.2:-75328971	MS.gene02572:CDS
GTTGTGAGCTTAACATCCAA+TGG	0.676653	3.2:-75329075	MS.gene02572:CDS
GGCAGCAAGATATGATGACA+TGG	0.679883	3.2:-75329103	MS.gene02572:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATGGACATATTAATATTG+TGG	-	chr3.2:75328923-75328942	MS.gene02572:CDS	20.0%
	AAAAGAATACACCTCTTCAT+TGG	-	chr3.2:75328991-75329010	MS.gene02572:CDS	30.0%
	AAAGAATACACCTCTTCATT+GGG	-	chr3.2:75328992-75329011	MS.gene02572:CDS	30.0%
	GGATCTTAATTCAGAAAATG+TGG	-	chr3.2:75328968-75328987	MS.gene02572:CDS	30.0%
	GTATTTAATCAGTAAAGGAG+CGG	-	chr3.2:75328947-75328966	MS.gene02572:CDS	30.0%
	GTGAAGAAATTAATCATTGC+TGG	-	chr3.2:75329041-75329060	MS.gene02572:CDS	30.0%
!	GTGGAGTATTTAATCAGTAA+AGG	-	chr3.2:75328942-75328961	MS.gene02572:CDS	30.0%
!!	GTGTTTTAAACAGTTATGAG+AGG	-	chr3.2:75329075-75329094	MS.gene02572:CDS	30.0%
	CGAAGATAACATCAATATCC+AGG	-	chr3.2:75328749-75328768	MS.gene02572:CDS	35.0%
	CTTAATTCCAAGGATGATCA+AGG	-	chr3.2:75328873-75328892	MS.gene02572:CDS	35.0%
	TGATCATCCTTGGAATTAAG+AGG	+	chr3.2:75328873-75328892	None:intergenic	35.0%
	AATGGATGAAGCTGTGACTA+GGG	-	chr3.2:75329103-75329122	MS.gene02572:CDS	40.0%
	AGGAAGAACTGCTCTTCATA+TGG	-	chr3.2:75328893-75328912	MS.gene02572:CDS	40.0%
	AGTTCTTCCTTGATCATCCT+TGG	+	chr3.2:75328883-75328902	None:intergenic	40.0%
	GAATATTGAAGCCTTGGTTG+AGG	-	chr3.2:75328794-75328813	MS.gene02572:CDS	40.0%
	GACAGAGAATATTGAAGCCT+TGG	-	chr3.2:75328788-75328807	MS.gene02572:CDS	40.0%
	GGAATTAAGAGGAACACCAT+TGG	+	chr3.2:75328862-75328881	None:intergenic	40.0%
	GGATGCAATTAATGAAGCAG+AGG	-	chr3.2:75329142-75329161	MS.gene02572:CDS	40.0%
	GTTGTGAGCTTAACATCCAA+TGG	-	chr3.2:75328843-75328862	MS.gene02572:CDS	40.0%
	TGGTGTTCCTCTTAATTCCA+AGG	-	chr3.2:75328863-75328882	MS.gene02572:CDS	40.0%
	ATATCTTGCTGCCTCAACCA+AGG	+	chr3.2:75328808-75328827	None:intergenic	45.0%
	ATGGATGAAGCTGTGACTAG+GGG	-	chr3.2:75329104-75329123	MS.gene02572:CDS	45.0%
	CAATGGATGAAGCTGTGACT+AGG	-	chr3.2:75329102-75329121	MS.gene02572:CDS	45.0%
	CAGTTATGAGAGGACTCCAA+TGG	-	chr3.2:75329085-75329104	MS.gene02572:CDS	45.0%
	CTAGTCACAGCTTCATCCAT+TGG	+	chr3.2:75329104-75329123	None:intergenic	45.0%
	CTTCATATGGCTGCAGCAAA+TGG	-	chr3.2:75328906-75328925	MS.gene02572:CDS	45.0%
	GGCAGCAAGATATGATGACA+TGG	-	chr3.2:75328815-75328834	MS.gene02572:CDS	45.0%
	TAGGGGCAAACTTGAAGTGA+TGG	-	chr3.2:75329121-75329140	MS.gene02572:CDS	45.0%
	TCTCTGTCATCGTCTCAGTT+GGG	+	chr3.2:75328776-75328795	None:intergenic	45.0%
	TTCTCTGTCATCGTCTCAGT+TGG	+	chr3.2:75328777-75328796	None:intergenic	45.0%
	AGTTGAACTTCGTGGTGCTC+TGG	-	chr3.2:75329169-75329188	MS.gene02572:CDS	50.0%
	CAACCTCAACATGCCCATTG+AGG	+	chr3.2:75329022-75329041	None:intergenic	50.0%
	TTCATTGGGCGTGCCTCAAT+GGG	-	chr3.2:75329006-75329025	MS.gene02572:CDS	50.0%
!	GAGGCACTAGTTGAACTTCG+TGG	-	chr3.2:75329161-75329180	MS.gene02572:CDS	50.0%
	CTTCATTGGGCGTGCCTCAA+TGG	-	chr3.2:75329005-75329024	MS.gene02572:CDS	55.0%
	GTGCCTCAATGGGCATGTTG+AGG	-	chr3.2:75329016-75329035	MS.gene02572:CDS	55.0%
	TCATCGTCTCAGTTGGGTCC+TGG	+	chr3.2:75328770-75328789	None:intergenic	55.0%
	TTGAGGCACGCCCAATGAAG+AGG	+	chr3.2:75329005-75329024	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	75328738	75329202	75328738	ID=MS.gene02572
chr3.2	mRNA	75328738	75329202	75328738	ID=MS.gene02572.t1;Parent=MS.gene02572
chr3.2	exon	75328738	75329202	75328738	ID=MS.gene02572.t1.exon1;Parent=MS.gene02572.t1
chr3.2	CDS	75328738	75329202	75328738	ID=cds.MS.gene02572.t1;Parent=MS.gene02572.t1

Gene Sequence

>MS.gene02572

ATGGGAGCAGTCGAAGATAACATCAATATCCAGGACCCAACTGAGACGATGACAGAGAATATTGAAGCCTTGGTTGAGGCAGCAAGATATGATGACATGGATGATGTTGTGAGCTTAACATCCAATGGTGTTCCTCTTAATTCCAAGGATGATCAAGGAAGAACTGCTCTTCATATGGCTGCAGCAAATGGACATATTAATATTGTGGAGTATTTAATCAGTAAAGGAGCGGATCTTAATTCAGAAAATGTGGAAAAGAATACACCTCTTCATTGGGCGTGCCTCAATGGGCATGTTGAGGTTGTGAAGAAATTAATCATTGCTGGAGCTAATGTTAGTGTTTTAAACAGTTATGAGAGGACTCCAATGGATGAAGCTGTGACTAGGGGCAAACTTGAAGTGATGGATGCAATTAATGAAGCAGAGGCACTAGTTGAACTTCGTGGTGCTCTGGTTTCTTCATAG

Protein sequence

>MS.gene02572.t1

MGAVEDNINIQDPTETMTENIEALVEAARYDDMDDVVSLTSNGVPLNSKDDQGRTALHMAAANGHINIVEYLISKGADLNSENVEKNTPLHWACLNGHVEVVKKLIIAGANVSVLNSYERTPMDEAVTRGKLEVMDAINEAEALVELRGALVSS