Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03201.t1 | XP_003602050.1 | 98.3 | 58 | 1 | 0 | 1 | 58 | 1 | 58 | 1.20E-22 | 116.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03201.t1 | G7LGL0 | 98.3 | 58 | 1 | 0 | 1 | 58 | 1 | 58 | 9.0e-23 | 116.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene03201 | MS.gene024713 | PPI |
| MS.gene03201 | MS.gene09762 | PPI |
| MS.gene03201 | MS.gene053224 | PPI |
| MS.gene03201 | MS.gene28384 | PPI |
| MS.gene03201 | MS.gene70215 | PPI |
| MS.gene03201 | MS.gene000610 | PPI |
| MS.gene03201 | MS.gene064850 | PPI |
| MS.gene57391 | MS.gene03201 | PPI |
| MS.gene29628 | MS.gene03201 | PPI |
| MS.gene03201 | MS.gene070008 | PPI |
| MS.gene22276 | MS.gene03201 | PPI |
| MS.gene03201 | MS.gene014333 | PPI |
| MS.gene03201 | MS.gene69319 | PPI |
| MS.gene03201 | MS.gene09779 | PPI |
| MS.gene03201 | MS.gene65180 | PPI |
| MS.gene03201 | MS.gene025830 | PPI |
| MS.gene064420 | MS.gene03201 | PPI |
| MS.gene02807 | MS.gene03201 | PPI |
| MS.gene03201 | MS.gene21936 | PPI |
| MS.gene03201 | MS.gene034249 | PPI |
| MS.gene03201 | MS.gene015411 | PPI |
| MS.gene03201 | MS.gene55165 | PPI |
| MS.gene80684 | MS.gene03201 | PPI |
| MS.gene80813 | MS.gene03201 | PPI |
| MS.gene030614 | MS.gene03201 | PPI |
| MS.gene03201 | MS.gene09780 | PPI |
| MS.gene21692 | MS.gene03201 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03201.t1 | MTR_3g088410 | 98.052 | 154 | 3 | 0 | 1 | 154 | 1 | 154 | 9.52e-110 | 308 |
| MS.gene03201.t1 | MTR_8g056020 | 37.984 | 129 | 80 | 0 | 19 | 147 | 228 | 356 | 3.83e-17 | 77.0 |
| MS.gene03201.t1 | MTR_5g026800 | 34.127 | 126 | 82 | 1 | 24 | 148 | 15 | 140 | 1.91e-15 | 71.6 |
| MS.gene03201.t1 | MTR_5g043640 | 38.889 | 90 | 55 | 0 | 38 | 127 | 108 | 197 | 3.57e-13 | 65.1 |
| MS.gene03201.t1 | MTR_5g043640 | 32.432 | 111 | 75 | 0 | 36 | 146 | 73 | 183 | 7.49e-11 | 58.5 |
| MS.gene03201.t1 | MTR_3g491870 | 34.783 | 92 | 60 | 0 | 21 | 112 | 238 | 329 | 7.52e-13 | 65.1 |
| MS.gene03201.t1 | MTR_1g089180 | 37.755 | 98 | 61 | 0 | 22 | 119 | 342 | 439 | 1.60e-12 | 64.3 |
| MS.gene03201.t1 | MTR_1g086410 | 60.000 | 45 | 18 | 0 | 47 | 91 | 11 | 55 | 7.40e-12 | 57.4 |
| MS.gene03201.t1 | MTR_6g027840 | 33.708 | 89 | 59 | 0 | 24 | 112 | 368 | 456 | 3.67e-11 | 60.5 |
| MS.gene03201.t1 | MTR_3g107720 | 34.211 | 114 | 73 | 1 | 27 | 140 | 543 | 654 | 4.48e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03201.t1 | AT5G12320 | 64.179 | 134 | 48 | 0 | 14 | 147 | 5 | 138 | 4.35e-60 | 182 |
| MS.gene03201.t1 | AT2G03430 | 35.714 | 126 | 77 | 1 | 19 | 140 | 45 | 170 | 4.41e-17 | 75.5 |
| MS.gene03201.t1 | AT2G03430 | 37.079 | 89 | 56 | 0 | 39 | 127 | 102 | 190 | 4.08e-11 | 59.3 |
| MS.gene03201.t1 | AT4G27780 | 32.381 | 105 | 71 | 0 | 20 | 124 | 233 | 337 | 4.19e-15 | 71.2 |
| MS.gene03201.t1 | AT4G35450 | 35.455 | 110 | 71 | 0 | 26 | 135 | 186 | 295 | 5.67e-15 | 70.9 |
| MS.gene03201.t1 | AT4G35450 | 35.455 | 110 | 71 | 0 | 26 | 135 | 224 | 333 | 8.50e-15 | 70.5 |
| MS.gene03201.t1 | AT4G35450 | 35.455 | 110 | 71 | 0 | 26 | 135 | 224 | 333 | 8.50e-15 | 70.5 |
| MS.gene03201.t1 | AT4G35450 | 35.455 | 110 | 71 | 0 | 26 | 135 | 224 | 333 | 8.50e-15 | 70.5 |
| MS.gene03201.t1 | AT4G35450 | 35.455 | 110 | 71 | 0 | 26 | 135 | 232 | 341 | 9.45e-15 | 70.5 |
| MS.gene03201.t1 | AT5G53470 | 31.429 | 105 | 72 | 0 | 20 | 124 | 218 | 322 | 1.48e-14 | 69.7 |
| MS.gene03201.t1 | AT4G19150 | 34.513 | 113 | 73 | 1 | 24 | 135 | 20 | 132 | 1.64e-14 | 68.6 |
| MS.gene03201.t1 | AT2G14255 | 33.929 | 112 | 71 | 2 | 6 | 116 | 12 | 121 | 3.67e-14 | 68.9 |
| MS.gene03201.t1 | AT3G09890 | 33.333 | 126 | 72 | 3 | 6 | 122 | 60 | 182 | 2.89e-12 | 62.0 |
| MS.gene03201.t1 | AT2G17390 | 31.818 | 110 | 75 | 0 | 26 | 135 | 226 | 335 | 8.55e-12 | 62.0 |
Find 37 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCATTGGGCGTGCCTCAA+TGG | 0.274476 | 3.3:-73804360 | MS.gene03201:CDS |
| TGGTGCTCTGGTTTCTTCAT+AGG | 0.336548 | 3.3:-73804184 | None:intergenic |
| TTCATTGGGCGTGCCTCAAT+GGG | 0.368210 | 3.3:-73804359 | MS.gene03201:CDS |
| GTGAAGAAATTAATCATTGC+TGG | 0.368237 | 3.3:-73804324 | MS.gene03201:CDS |
| AAAAGAATACACCTCTTCAT+TGG | 0.393239 | 3.3:-73804374 | MS.gene03201:CDS |
| CTTCATATGGCTGCAGCAAA+TGG | 0.395615 | 3.3:-73804459 | MS.gene03201:CDS |
| TGATCATCCTTGGAATTAAG+AGG | 0.420728 | 3.3:+73804495 | None:intergenic |
| AGTTCTTCCTTGATCATCCT+TGG | 0.441829 | 3.3:+73804485 | None:intergenic |
| TTCTCTGTCATCGTCTCAGT+TGG | 0.444663 | 3.3:+73804591 | None:intergenic |
| TCATCGTCTCAGTTGGGTCC+TGG | 0.448050 | 3.3:+73804598 | None:intergenic |
| AGTTGAACTTCGTGGTGCTC+TGG | 0.450551 | 3.3:-73804196 | MS.gene03201:CDS |
| AATGGATGAAGCTGTGACTA+GGG | 0.455886 | 3.3:-73804262 | MS.gene03201:CDS |
| AGGAAGAACTGCTCTTCATA+TGG | 0.461895 | 3.3:-73804472 | MS.gene03201:CDS |
| CAATGGATGAAGCTGTGACT+AGG | 0.469105 | 3.3:-73804263 | MS.gene03201:CDS |
| AAAGAATACACCTCTTCATT+GGG | 0.493944 | 3.3:-73804373 | MS.gene03201:CDS |
| CTAGTCACAGCTTCATCCAT+TGG | 0.498266 | 3.3:+73804264 | None:intergenic |
| GTGGAGTATTTAATCAGTAA+AGG | 0.499037 | 3.3:-73804423 | MS.gene03201:CDS |
| CGAAGATAACATCAATATCC+AGG | 0.509750 | 3.3:-73804616 | MS.gene03201:CDS |
| CTTAATTCCAAGGATGATCA+AGG | 0.510692 | 3.3:-73804492 | MS.gene03201:CDS |
| TGGTGTTCCTCTTAATTCCA+AGG | 0.535842 | 3.3:-73804502 | MS.gene03201:CDS |
| TCTCTGTCATCGTCTCAGTT+GGG | 0.542518 | 3.3:+73804592 | None:intergenic |
| TAGGGGCAAACTTGAAGTGA+TGG | 0.585162 | 3.3:-73804244 | MS.gene03201:CDS |
| GGAATTAAGAGGAACACCAT+TGG | 0.586019 | 3.3:+73804506 | None:intergenic |
| GAGGCACTAGTTGAACTTCG+TGG | 0.586185 | 3.3:-73804204 | MS.gene03201:CDS |
| TTGAGGCACGCCCAATGAAG+AGG | 0.592105 | 3.3:+73804363 | None:intergenic |
| GAATATTGAAGCCTTGCTTG+AGG | 0.596172 | 3.3:-73804571 | MS.gene03201:CDS |
| AAATGGACATATTAATATTG+TGG | 0.599091 | 3.3:-73804442 | MS.gene03201:CDS |
| CAACCTCAACATGCCCATTG+AGG | 0.600476 | 3.3:+73804346 | None:intergenic |
| CAGTTATGAGAGGACTCCAA+TGG | 0.604606 | 3.3:-73804280 | MS.gene03201:CDS |
| ATATCTTGCTGCCTCAAGCA+AGG | 0.614678 | 3.3:+73804560 | None:intergenic |
| GTGCCTCAATGGGCATGTTG+AGG | 0.622233 | 3.3:-73804349 | MS.gene03201:CDS |
| GGATCTTAATTCAGAAAATG+TGG | 0.626131 | 3.3:-73804397 | MS.gene03201:CDS |
| GGATGCAATTAATGAAGCAG+AGG | 0.634709 | 3.3:-73804223 | MS.gene03201:CDS |
| ATGGATGAAGCTGTGACTAG+GGG | 0.659988 | 3.3:-73804261 | MS.gene03201:CDS |
| GTATTTAATCAGTAAAGGAG+CGG | 0.669015 | 3.3:-73804418 | MS.gene03201:CDS |
| GTTGTGAGCTTAACATCCAA+TGG | 0.676653 | 3.3:-73804522 | MS.gene03201:CDS |
| GGCAGCAAGATATGATGACA+TGG | 0.679883 | 3.3:-73804550 | MS.gene03201:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATGGACATATTAATATTG+TGG | - | chr3.3:73804370-73804389 | MS.gene03201:CDS | 20.0% |
| AAAAGAATACACCTCTTCAT+TGG | - | chr3.3:73804438-73804457 | MS.gene03201:CDS | 30.0% | |
| AAAGAATACACCTCTTCATT+GGG | - | chr3.3:73804439-73804458 | MS.gene03201:CDS | 30.0% | |
| GGATCTTAATTCAGAAAATG+TGG | - | chr3.3:73804415-73804434 | MS.gene03201:CDS | 30.0% | |
| GTATTTAATCAGTAAAGGAG+CGG | - | chr3.3:73804394-73804413 | MS.gene03201:CDS | 30.0% | |
| GTGAAGAAATTAATCATTGC+TGG | - | chr3.3:73804488-73804507 | MS.gene03201:CDS | 30.0% | |
| ! | GTGGAGTATTTAATCAGTAA+AGG | - | chr3.3:73804389-73804408 | MS.gene03201:CDS | 30.0% |
| !! | GTGTTTTAAACAGTTATGAG+AGG | - | chr3.3:73804522-73804541 | MS.gene03201:CDS | 30.0% |
| CGAAGATAACATCAATATCC+AGG | - | chr3.3:73804196-73804215 | MS.gene03201:CDS | 35.0% | |
| CTTAATTCCAAGGATGATCA+AGG | - | chr3.3:73804320-73804339 | MS.gene03201:CDS | 35.0% | |
| TGATCATCCTTGGAATTAAG+AGG | + | chr3.3:73804320-73804339 | None:intergenic | 35.0% | |
| AATGGATGAAGCTGTGACTA+GGG | - | chr3.3:73804550-73804569 | MS.gene03201:CDS | 40.0% | |
| AGGAAGAACTGCTCTTCATA+TGG | - | chr3.3:73804340-73804359 | MS.gene03201:CDS | 40.0% | |
| AGTTCTTCCTTGATCATCCT+TGG | + | chr3.3:73804330-73804349 | None:intergenic | 40.0% | |
| GAATATTGAAGCCTTGCTTG+AGG | - | chr3.3:73804241-73804260 | MS.gene03201:CDS | 40.0% | |
| GGAATTAAGAGGAACACCAT+TGG | + | chr3.3:73804309-73804328 | None:intergenic | 40.0% | |
| GGATGCAATTAATGAAGCAG+AGG | - | chr3.3:73804589-73804608 | MS.gene03201:CDS | 40.0% | |
| GTTGTGAGCTTAACATCCAA+TGG | - | chr3.3:73804290-73804309 | MS.gene03201:CDS | 40.0% | |
| TGGTGTTCCTCTTAATTCCA+AGG | - | chr3.3:73804310-73804329 | MS.gene03201:CDS | 40.0% | |
| ATATCTTGCTGCCTCAAGCA+AGG | + | chr3.3:73804255-73804274 | None:intergenic | 45.0% | |
| ATGGATGAAGCTGTGACTAG+GGG | - | chr3.3:73804551-73804570 | MS.gene03201:CDS | 45.0% | |
| CAATGGATGAAGCTGTGACT+AGG | - | chr3.3:73804549-73804568 | MS.gene03201:CDS | 45.0% | |
| CAGTTATGAGAGGACTCCAA+TGG | - | chr3.3:73804532-73804551 | MS.gene03201:CDS | 45.0% | |
| CTAGTCACAGCTTCATCCAT+TGG | + | chr3.3:73804551-73804570 | None:intergenic | 45.0% | |
| CTTCATATGGCTGCAGCAAA+TGG | - | chr3.3:73804353-73804372 | MS.gene03201:CDS | 45.0% | |
| GGCAGCAAGATATGATGACA+TGG | - | chr3.3:73804262-73804281 | MS.gene03201:CDS | 45.0% | |
| TAGGGGCAAACTTGAAGTGA+TGG | - | chr3.3:73804568-73804587 | MS.gene03201:CDS | 45.0% | |
| TCTCTGTCATCGTCTCAGTT+GGG | + | chr3.3:73804223-73804242 | None:intergenic | 45.0% | |
| TTCTCTGTCATCGTCTCAGT+TGG | + | chr3.3:73804224-73804243 | None:intergenic | 45.0% | |
| AGTTGAACTTCGTGGTGCTC+TGG | - | chr3.3:73804616-73804635 | MS.gene03201:CDS | 50.0% | |
| CAACCTCAACATGCCCATTG+AGG | + | chr3.3:73804469-73804488 | None:intergenic | 50.0% | |
| TTCATTGGGCGTGCCTCAAT+GGG | - | chr3.3:73804453-73804472 | MS.gene03201:CDS | 50.0% | |
| ! | GAGGCACTAGTTGAACTTCG+TGG | - | chr3.3:73804608-73804627 | MS.gene03201:CDS | 50.0% |
| CTTCATTGGGCGTGCCTCAA+TGG | - | chr3.3:73804452-73804471 | MS.gene03201:CDS | 55.0% | |
| GTGCCTCAATGGGCATGTTG+AGG | - | chr3.3:73804463-73804482 | MS.gene03201:CDS | 55.0% | |
| TCATCGTCTCAGTTGGGTCC+TGG | + | chr3.3:73804217-73804236 | None:intergenic | 55.0% | |
| TTGAGGCACGCCCAATGAAG+AGG | + | chr3.3:73804452-73804471 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 73804185 | 73804649 | 73804185 | ID=MS.gene03201 |
| chr3.3 | mRNA | 73804185 | 73804649 | 73804185 | ID=MS.gene03201.t1;Parent=MS.gene03201 |
| chr3.3 | exon | 73804185 | 73804649 | 73804185 | ID=MS.gene03201.t1.exon1;Parent=MS.gene03201.t1 |
| chr3.3 | CDS | 73804185 | 73804649 | 73804185 | ID=cds.MS.gene03201.t1;Parent=MS.gene03201.t1 |
| Gene Sequence |
| Protein sequence |
