Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21917.t1 | XP_003610915.2 | 99.4 | 333 | 2 | 0 | 1 | 333 | 1 | 333 | 2.80E-185 | 657.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21917.t1 | Q8BVQ5 | 45.0 | 373 | 148 | 8 | 3 | 326 | 7 | 371 | 2.4e-76 | 287.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21917.t1 | G7K7W8 | 99.4 | 333 | 2 | 0 | 1 | 333 | 1 | 333 | 2.0e-185 | 657.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051234 | MS.gene21917 | 0.801851 | 7.41E-49 | -1.69E-46 |
| MS.gene058149 | MS.gene21917 | 0.862248 | 5.80E-64 | -1.69E-46 |
| MS.gene05894 | MS.gene21917 | 0.806539 | 7.83E-50 | -1.69E-46 |
| MS.gene059778 | MS.gene21917 | 0.822932 | 1.82E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21917.t1 | MTR_5g008450 | 99.399 | 333 | 2 | 0 | 1 | 333 | 1 | 333 | 0.0 | 686 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21917.t1 | AT4G10050 | 76.877 | 333 | 74 | 2 | 3 | 332 | 4 | 336 | 0.0 | 546 |
| MS.gene21917.t1 | AT4G10050 | 82.955 | 264 | 45 | 0 | 69 | 332 | 1 | 264 | 1.27e-168 | 472 |
Find 99 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGACTCCATTAGGGCCTATT+TGG | 0.179803 | 5.1:-2457979 | None:intergenic |
| TTACACGGTGGTGGTTATTC+TGG | 0.180394 | 5.1:+2453061 | MS.gene21917:CDS |
| CCAGCAGCAGTTGCCGTTCT+TGG | 0.199984 | 5.1:-2459946 | None:intergenic |
| AAACTAACATCATTTGTTTC+TGG | 0.230576 | 5.1:-2452901 | None:intergenic |
| GTTTATCCAACACCTGATTC+AGG | 0.254969 | 5.1:-2459924 | None:intergenic |
| TTTATTGCTCGTAACCAAAT+AGG | 0.289071 | 5.1:+2457965 | MS.gene21917:CDS |
| ACACACAGGGCATGCTATTC+AGG | 0.324713 | 5.1:+2457807 | MS.gene21917:CDS |
| GGTTTCGAAAAGGCAGGCTT+GGG | 0.333613 | 5.1:-2459902 | None:intergenic |
| AGCCATCAAATCTATGATAA+TGG | 0.334190 | 5.1:+2458719 | MS.gene21917:CDS |
| ATACATACCACGACTCCATT+AGG | 0.334656 | 5.1:-2457989 | None:intergenic |
| TGATGTATTGGCTGTTATAA+AGG | 0.347563 | 5.1:+2454036 | MS.gene21917:CDS |
| AACCATTATCATAGATTTGA+TGG | 0.353082 | 5.1:-2458721 | None:intergenic |
| AAATCATTGTCAACCTTGGC+TGG | 0.369006 | 5.1:+2455138 | MS.gene21917:CDS |
| CCAAGAACGGCAACTGCTGC+TGG | 0.379637 | 5.1:+2459946 | MS.gene21917:CDS |
| CTTTCATTTGCTGTGTCAAC+GGG | 0.387831 | 5.1:+2453819 | MS.gene21917:CDS |
| TACACGGTGGTGGTTATTCT+GGG | 0.388188 | 5.1:+2453062 | MS.gene21917:CDS |
| TGCAGCAAATCCTCTCAAAT+AGG | 0.401973 | 5.1:+2455315 | MS.gene21917:CDS |
| ATAGACAATGAATCTCAAAC+TGG | 0.402842 | 5.1:-2458694 | None:intergenic |
| TGGAAAAGACTGAACAATAT+TGG | 0.403174 | 5.1:+2455872 | MS.gene21917:CDS |
| AATATGATGATTCGAAGAAA+TGG | 0.403489 | 5.1:+2455688 | MS.gene21917:CDS |
| AATAACAACAGCTTCGGAAC+AGG | 0.404928 | 5.1:-2457287 | None:intergenic |
| ATAATGGTTGCCTTCTTTGA+GGG | 0.414214 | 5.1:+2458735 | MS.gene21917:CDS |
| GCTGTTATAAAGGAATTATA+TGG | 0.414492 | 5.1:+2454046 | MS.gene21917:CDS |
| CCGAAGCTGTTGTTATTAGC+AGG | 0.418860 | 5.1:+2457293 | MS.gene21917:CDS |
| GTTATATTGTAGGAGTTCTT+CGG | 0.419029 | 5.1:+2452819 | MS.gene21917:intron |
| TTGATCGTCGGTGATATCTT+CGG | 0.419891 | 5.1:-2452668 | None:intergenic |
| CGTAACCAAATAGGCCCTAA+TGG | 0.420014 | 5.1:+2457974 | MS.gene21917:CDS |
| GGGTTTCGAAAAGGCAGGCT+TGG | 0.436641 | 5.1:-2459903 | None:intergenic |
| GATAATGGTTGCCTTCTTTG+AGG | 0.438523 | 5.1:+2458734 | MS.gene21917:CDS |
| AGAAATACGCACCGTTGGAT+TGG | 0.446308 | 5.1:+2452842 | MS.gene21917:CDS |
| TGTAACAGGTCTCTCACAAT+TGG | 0.451447 | 5.1:+2457748 | MS.gene21917:intron |
| TACATACCACGACTCCATTA+GGG | 0.451852 | 5.1:-2457988 | None:intergenic |
| ATTAGCAGGAACAGATAGAT+TGG | 0.456800 | 5.1:+2457307 | MS.gene21917:CDS |
| CTGAACAATATTGGAAAGGC+TGG | 0.457718 | 5.1:+2455881 | MS.gene21917:CDS |
| CTTGGCTGGGTTGATTGTTG+TGG | 0.462285 | 5.1:+2455152 | MS.gene21917:CDS |
| TGATCGTCGGTGATATCTTC+GGG | 0.464430 | 5.1:-2452667 | None:intergenic |
| TTGAGGAACAGAAGCGAAAA+CGG | 0.465204 | 5.1:-2452707 | None:intergenic |
| TGACCAACGAGGATAATTGC+TGG | 0.466211 | 5.1:-2454079 | None:intergenic |
| AAGGCAGGCTTGGGGAGGCC+AGG | 0.476762 | 5.1:-2459893 | None:intergenic |
| GTATAATTAAGGAGAAAGCT+AGG | 0.484847 | 5.1:+2453841 | MS.gene21917:CDS |
| TTGCTTGTATGCAGATTGAA+TGG | 0.491842 | 5.1:+2455601 | MS.gene21917:intron |
| GATTGTTGTGGATGTTGTAG+AGG | 0.492777 | 5.1:+2455164 | MS.gene21917:CDS |
| CGAAACCCTGAATCAGGTGT+TGG | 0.493079 | 5.1:+2459918 | MS.gene21917:CDS |
| TTTATCCAACACCTGATTCA+GGG | 0.495087 | 5.1:-2459923 | None:intergenic |
| ACAAGAGTTGCAAATTCATC+AGG | 0.495103 | 5.1:-2457935 | None:intergenic |
| CCGTGTAAACAAAATACAAC+AGG | 0.497807 | 5.1:-2453046 | None:intergenic |
| GCTTTCATTTGCTGTGTCAA+CGG | 0.504011 | 5.1:+2453818 | MS.gene21917:CDS |
| CCTGATTCAGGGTTTCGAAA+AGG | 0.508303 | 5.1:-2459912 | None:intergenic |
| ACAGAAGCGAAAACGGAAGC+AGG | 0.511886 | 5.1:-2452700 | None:intergenic |
| GACCATGTGCAATGATGTAT+TGG | 0.512442 | 5.1:+2454024 | MS.gene21917:intron |
| TGTGTCAACGGGTATAATTA+AGG | 0.520801 | 5.1:+2453830 | MS.gene21917:CDS |
| TTCGGAGAAATACGCACCGT+TGG | 0.529265 | 5.1:+2452837 | MS.gene21917:CDS |
| ATTCAGGGTTTCGAAAAGGC+AGG | 0.532832 | 5.1:-2459908 | None:intergenic |
| TTATGTTTATCGAACAGAGC+TGG | 0.535307 | 5.1:+2455852 | MS.gene21917:CDS |
| CAATTATCCTCGTTGGTCAC+AGG | 0.536543 | 5.1:+2454083 | MS.gene21917:CDS |
| CCTCCAGCAATTATCCTCGT+TGG | 0.537076 | 5.1:+2454076 | MS.gene21917:CDS |
| AATGTTGCATCCTATTTGAG+AGG | 0.540775 | 5.1:-2455325 | None:intergenic |
| CAAGAACGGCAACTGCTGCT+GGG | 0.543535 | 5.1:+2459947 | MS.gene21917:CDS |
| TCGAAAAGGCAGGCTTGGGG+AGG | 0.546492 | 5.1:-2459898 | None:intergenic |
| CCTGCTAATAACAACAGCTT+CGG | 0.548805 | 5.1:-2457293 | None:intergenic |
| GAAAGCTAGGGTGGTTGCGA+TGG | 0.548883 | 5.1:+2453854 | MS.gene21917:CDS |
| GCGAAAACGGAAGCAGGTTG+TGG | 0.551197 | 5.1:-2452694 | None:intergenic |
| ATGATAGTTGTCAGACACAC+AGG | 0.553607 | 5.1:+2457793 | MS.gene21917:CDS |
| CTCACAATTGGTCAAATGCA+AGG | 0.555981 | 5.1:+2457760 | MS.gene21917:CDS |
| TACTTAGTTGGAGGACGTTG+AGG | 0.562835 | 5.1:-2452724 | None:intergenic |
| AAAGGTGGTTACTTAGTTGG+AGG | 0.568541 | 5.1:-2452733 | None:intergenic |
| GCGATGGACTTGAGAGGTCA+TGG | 0.569848 | 5.1:+2453870 | MS.gene21917:CDS |
| AAGGAAATCATTGTCAACCT+TGG | 0.570433 | 5.1:+2455134 | MS.gene21917:CDS |
| AATCATTGTCAACCTTGGCT+GGG | 0.570477 | 5.1:+2455139 | MS.gene21917:CDS |
| AGAATTTCAGGGAACAGCAA+TGG | 0.571928 | 5.1:+2455277 | MS.gene21917:intron |
| ATTGAATGGAGTGTGAGGGC+TGG | 0.573026 | 5.1:+2455615 | MS.gene21917:CDS |
| TGTTTACATGGCAGGAACTG+AGG | 0.579675 | 5.1:+2453021 | MS.gene21917:CDS |
| TATAATTAAGGAGAAAGCTA+GGG | 0.580788 | 5.1:+2453842 | MS.gene21917:CDS |
| ACATAGCATACCCTCAAAGA+AGG | 0.584416 | 5.1:-2458745 | None:intergenic |
| GTTTACATGGCAGGAACTGA+GGG | 0.586652 | 5.1:+2453022 | MS.gene21917:CDS |
| AAGACTGAACAATATTGGAA+AGG | 0.599196 | 5.1:+2455877 | MS.gene21917:CDS |
| AATTAAGGAGAAAGCTAGGG+TGG | 0.599249 | 5.1:+2453845 | MS.gene21917:CDS |
| GGTGTTGGATAAACCAAGAA+CGG | 0.608439 | 5.1:+2459933 | MS.gene21917:CDS |
| CAGGAACAGATAGATTGGAC+AGG | 0.610230 | 5.1:+2457312 | MS.gene21917:CDS |
| CACAACAATCAACCCAGCCA+AGG | 0.611749 | 5.1:-2455151 | None:intergenic |
| ATAACAACAGCTTCGGAACA+GGG | 0.614518 | 5.1:-2457286 | None:intergenic |
| ATCCATCATCAAAGCATGGG+GGG | 0.618423 | 5.1:+2455087 | MS.gene21917:intron |
| TCCATCATCAAAGCATGGGG+GGG | 0.626873 | 5.1:+2455088 | MS.gene21917:intron |
| AAAACGGAAGCAGGTTGTGG+TGG | 0.628241 | 5.1:-2452691 | None:intergenic |
| GTTTCGAAAAGGCAGGCTTG+GGG | 0.636712 | 5.1:-2459901 | None:intergenic |
| TAACAACAGCTTCGGAACAG+GGG | 0.644743 | 5.1:-2457285 | None:intergenic |
| TAGACAATGAATCTCAAACT+GGG | 0.648560 | 5.1:-2458693 | None:intergenic |
| AGGTTGTGGTGGTTGATCGT+CGG | 0.658759 | 5.1:-2452680 | None:intergenic |
| AATAGGCCCTAATGGAGTCG+TGG | 0.668041 | 5.1:+2457982 | MS.gene21917:CDS |
| TGCAGATTGAATGGAGTGTG+AGG | 0.671060 | 5.1:+2455610 | MS.gene21917:intron |
| TGATAGTTGTCAGACACACA+GGG | 0.673323 | 5.1:+2457794 | MS.gene21917:CDS |
| AGCCAATACATCATTGCACA+TGG | 0.678025 | 5.1:-2454026 | None:intergenic |
| GTGGTTGCGATGGACTTGAG+AGG | 0.687131 | 5.1:+2453864 | MS.gene21917:CDS |
| AAATACACAGACCAATCCAA+CGG | 0.692479 | 5.1:-2452853 | None:intergenic |
| TTGCTGTGCATATTGCTGCA+AGG | 0.693525 | 5.1:+2455115 | MS.gene21917:CDS |
| TATCCATCATCAAAGCATGG+GGG | 0.700379 | 5.1:+2455086 | MS.gene21917:intron |
| GCAGATTGAATGGAGTGTGA+GGG | 0.705258 | 5.1:+2455611 | MS.gene21917:intron |
| CCAACGAGGATAATTGCTGG+AGG | 0.728227 | 5.1:-2454076 | None:intergenic |
| TAAATCACCTGTGACCAACG+AGG | 0.765150 | 5.1:-2454090 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 2452640 | 2459972 | 2452640 | ID=MS.gene21917 |
| chr5.1 | mRNA | 2452640 | 2459972 | 2452640 | ID=MS.gene21917.t1;Parent=MS.gene21917 |
| chr5.1 | exon | 2452640 | 2452743 | 2452640 | ID=MS.gene21917.t1.exon1;Parent=MS.gene21917.t1 |
| chr5.1 | CDS | 2452640 | 2452743 | 2452640 | ID=cds.MS.gene21917.t1;Parent=MS.gene21917.t1 |
| chr5.1 | exon | 2452831 | 2452915 | 2452831 | ID=MS.gene21917.t1.exon2;Parent=MS.gene21917.t1 |
| chr5.1 | CDS | 2452831 | 2452915 | 2452831 | ID=cds.MS.gene21917.t1;Parent=MS.gene21917.t1 |
| chr5.1 | exon | 2453013 | 2453083 | 2453013 | ID=MS.gene21917.t1.exon3;Parent=MS.gene21917.t1 |
| chr5.1 | CDS | 2453013 | 2453083 | 2453013 | ID=cds.MS.gene21917.t1;Parent=MS.gene21917.t1 |
| chr5.1 | exon | 2453818 | 2453932 | 2453818 | ID=MS.gene21917.t1.exon4;Parent=MS.gene21917.t1 |
| chr5.1 | CDS | 2453818 | 2453932 | 2453818 | ID=cds.MS.gene21917.t1;Parent=MS.gene21917.t1 |
| chr5.1 | exon | 2454025 | 2454104 | 2454025 | ID=MS.gene21917.t1.exon5;Parent=MS.gene21917.t1 |
| chr5.1 | CDS | 2454025 | 2454104 | 2454025 | ID=cds.MS.gene21917.t1;Parent=MS.gene21917.t1 |
| chr5.1 | exon | 2455101 | 2455185 | 2455101 | ID=MS.gene21917.t1.exon6;Parent=MS.gene21917.t1 |
| chr5.1 | CDS | 2455101 | 2455185 | 2455101 | ID=cds.MS.gene21917.t1;Parent=MS.gene21917.t1 |
| chr5.1 | exon | 2455287 | 2455367 | 2455287 | ID=MS.gene21917.t1.exon7;Parent=MS.gene21917.t1 |
| chr5.1 | CDS | 2455287 | 2455367 | 2455287 | ID=cds.MS.gene21917.t1;Parent=MS.gene21917.t1 |
| chr5.1 | exon | 2455615 | 2455709 | 2455615 | ID=MS.gene21917.t1.exon8;Parent=MS.gene21917.t1 |
| chr5.1 | CDS | 2455615 | 2455709 | 2455615 | ID=cds.MS.gene21917.t1;Parent=MS.gene21917.t1 |
| chr5.1 | exon | 2455852 | 2455902 | 2455852 | ID=MS.gene21917.t1.exon9;Parent=MS.gene21917.t1 |
| chr5.1 | CDS | 2455852 | 2455902 | 2455852 | ID=cds.MS.gene21917.t1;Parent=MS.gene21917.t1 |
| chr5.1 | exon | 2457253 | 2457333 | 2457253 | ID=MS.gene21917.t1.exon10;Parent=MS.gene21917.t1 |
| chr5.1 | CDS | 2457253 | 2457333 | 2457253 | ID=cds.MS.gene21917.t1;Parent=MS.gene21917.t1 |
| chr5.1 | exon | 2457756 | 2457828 | 2457756 | ID=MS.gene21917.t1.exon11;Parent=MS.gene21917.t1 |
| chr5.1 | CDS | 2457756 | 2457828 | 2457756 | ID=cds.MS.gene21917.t1;Parent=MS.gene21917.t1 |
| chr5.1 | exon | 2457926 | 2458003 | 2457926 | ID=MS.gene21917.t1.exon12;Parent=MS.gene21917.t1 |
| chr5.1 | CDS | 2457926 | 2458003 | 2457926 | ID=cds.MS.gene21917.t1;Parent=MS.gene21917.t1 |
| chr5.1 | exon | 2458693 | 2458756 | 2458693 | ID=MS.gene21917.t1.exon13;Parent=MS.gene21917.t1 |
| chr5.1 | CDS | 2458693 | 2458756 | 2458693 | ID=cds.MS.gene21917.t1;Parent=MS.gene21917.t1 |
| chr5.1 | exon | 2459890 | 2459972 | 2459890 | ID=MS.gene21917.t1.exon14;Parent=MS.gene21917.t1 |
| chr5.1 | CDS | 2459890 | 2459972 | 2459890 | ID=cds.MS.gene21917.t1;Parent=MS.gene21917.t1 |
| Gene Sequence |
| Protein sequence |
