AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21936

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21936.t1 MTR_5g008790 94.545 165 6 2 1 165 1 162 1.54e-112 316
MS.gene21936.t1 MTR_1g014860 43.519 108 55 2 1 104 5 110 7.15e-25 95.9
MS.gene21936.t1 MTR_1g014800 42.609 115 56 3 1 107 10 122 1.60e-23 92.4
MS.gene21936.t1 MTR_1g014820 42.857 105 57 2 1 104 10 112 1.78e-23 93.2
MS.gene21936.t1 MTR_5g016750 45.000 100 48 3 13 109 20 115 2.75e-23 90.9
MS.gene21936.t1 MTR_3g102100 43.636 110 51 3 16 118 43 148 1.29e-22 89.0
MS.gene21936.t1 MTR_1g019110 46.465 99 46 2 16 109 42 138 3.41e-22 87.8
MS.gene21936.t1 MTR_1g014780 43.617 94 47 2 15 104 35 126 4.79e-22 89.0
MS.gene21936.t1 MTR_3g105510 46.667 90 45 2 16 104 35 122 7.39e-22 88.6
MS.gene21936.t1 MTR_3g072610 53.000 100 42 2 7 104 14 110 8.81e-22 87.8
MS.gene21936.t1 MTR_5g058470 54.255 94 42 1 6 99 84 176 2.04e-21 88.6
MS.gene21936.t1 MTR_2g101340 53.750 80 32 2 18 97 41 115 6.75e-21 85.1
MS.gene21936.t1 MTR_8g102800 51.163 86 37 2 12 97 31 111 1.96e-20 84.7
MS.gene21936.t1 MTR_8g027465 48.750 80 39 1 18 97 39 116 3.38e-20 82.8
MS.gene21936.t1 MTR_3g110205 47.126 87 41 2 18 104 43 124 5.15e-20 82.4
MS.gene21936.t1 MTR_5g012910 47.826 92 41 3 8 97 16 102 1.90e-19 80.9
MS.gene21936.t1 MTR_7g117690 58.333 60 23 1 18 77 28 85 2.21e-19 80.5
MS.gene21936.t1 MTR_6g088405 56.250 80 30 3 18 97 44 118 3.54e-19 80.9
MS.gene21936.t1 MTR_3g105480 45.349 86 44 2 20 104 33 116 3.65e-19 81.3
MS.gene21936.t1 MTR_4g100650 47.619 84 39 2 14 97 70 148 5.26e-19 81.3
MS.gene21936.t1 MTR_2g043050 39.806 103 55 3 2 104 5 100 8.94e-18 77.4
MS.gene21936.t1 MTR_7g028925 46.753 77 36 2 1 77 12 83 1.13e-17 73.9
MS.gene21936.t1 MTR_8g013860 44.211 95 48 2 14 108 43 132 1.57e-17 76.3
MS.gene21936.t1 MTR_1g090170 53.968 63 27 1 16 78 150 210 1.95e-17 77.8
MS.gene21936.t1 MTR_7g046260 45.977 87 42 2 11 97 54 135 2.28e-17 76.6
MS.gene21936.t1 MTR_1g090170 53.968 63 27 1 16 78 237 297 2.80e-17 78.2
MS.gene21936.t1 MTR_5g083340 51.429 70 32 1 17 86 84 151 7.13e-17 77.0
MS.gene21936.t1 MTR_5g062700 36.893 103 59 2 16 118 171 267 3.95e-16 74.7
MS.gene21936.t1 MTR_8g006410 47.297 74 37 1 6 79 40 111 4.50e-16 72.4
MS.gene21936.t1 MTR_2g043030 44.286 70 37 1 8 77 9 76 5.25e-16 72.4
MS.gene21936.t1 MTR_3g074130 48.571 70 34 1 9 78 141 208 1.04e-15 73.2
MS.gene21936.t1 MTR_8g090350 52.381 63 28 1 16 78 111 171 1.36e-15 72.4
MS.gene21936.t1 MTR_2g043040 50.000 60 28 1 18 77 19 76 1.41e-15 71.6
MS.gene21936.t1 MTR_4g035430 40.816 98 53 2 7 104 21 113 2.15e-15 70.9
MS.gene21936.t1 MTR_2g085015 43.820 89 43 4 18 104 47 130 4.01e-15 70.5
MS.gene21936.t1 MTR_7g021010 42.222 90 37 2 1 77 21 108 4.92e-15 69.3
MS.gene21936.t1 MTR_5g008470 51.667 60 27 1 20 79 77 134 5.57e-15 69.3
MS.gene21936.t1 MTR_3g090760 40.196 102 53 2 13 114 108 201 6.18e-15 71.2
MS.gene21936.t1 MTR_2g090235 50.000 64 30 1 16 79 216 277 7.83e-15 71.2
MS.gene21936.t1 MTR_7g020980 53.333 60 26 1 18 77 51 108 2.14e-14 67.4
MS.gene21936.t1 MTR_2g043020 41.429 70 39 1 8 77 9 76 2.42e-14 67.8
MS.gene21936.t1 MTR_5g008540 51.667 60 27 1 20 79 26 83 3.31e-14 65.1
MS.gene21936.t1 MTR_7g021010 51.667 60 27 1 18 77 12 69 4.49e-14 65.9
MS.gene21936.t1 MTR_5g010940 54.545 66 22 2 18 77 54 117 7.29e-14 67.0
MS.gene21936.t1 MTR_2g089170 49.180 61 29 1 18 78 65 123 7.62e-14 66.2
MS.gene21936.t1 MTR_1g101600 53.333 60 26 1 18 77 41 98 8.20e-14 66.6
MS.gene21936.t1 MTR_7g081815 49.254 67 31 2 16 81 59 123 9.23e-14 66.2
MS.gene21936.t1 MTR_4g086165 51.613 62 28 1 15 76 79 138 1.11e-13 66.2
MS.gene21936.t1 MTR_7g081805 49.254 67 31 2 16 81 59 123 1.30e-13 65.9
MS.gene21936.t1 MTR_5g008550 54.839 62 26 1 18 79 21 80 1.42e-13 65.5
MS.gene21936.t1 MTR_5g010910 54.545 66 22 2 18 77 49 112 1.44e-13 66.2
MS.gene21936.t1 MTR_5g008590 55.738 61 25 1 18 78 11 69 1.47e-13 65.5
MS.gene21936.t1 MTR_4g010640 52.459 61 27 1 16 76 5 63 1.64e-13 65.9
MS.gene21936.t1 MTR_4g114570 51.613 62 28 1 15 76 4 63 1.96e-13 65.5
MS.gene21936.t1 MTR_5g010930 53.030 66 23 2 18 77 49 112 2.26e-13 65.9
MS.gene21936.t1 MTR_2g103700 50.794 63 29 1 14 76 3 63 2.43e-13 64.7
MS.gene21936.t1 MTR_7g092190 50.000 62 29 1 17 78 29 88 3.04e-13 66.2
MS.gene21936.t1 MTR_6g465450 51.667 60 27 1 18 77 54 111 3.17e-13 65.5
MS.gene21936.t1 MTR_0939s0010 45.902 61 31 1 18 78 65 123 3.20e-13 64.7
MS.gene21936.t1 MTR_6g465420 51.667 60 27 1 18 77 54 111 3.39e-13 65.9
MS.gene21936.t1 MTR_5g009620 42.857 70 40 0 16 85 110 179 3.46e-13 66.2
MS.gene21936.t1 MTR_1g028560 57.778 45 19 0 16 60 113 157 4.86e-13 65.9
MS.gene21936.t1 MTR_5g083330 50.000 62 29 1 17 78 34 93 4.93e-13 65.5
MS.gene21936.t1 MTR_2g014300 38.272 81 46 2 6 84 3 81 5.19e-13 63.9
MS.gene21936.t1 MTR_4g102670 49.254 67 31 2 18 84 15 78 5.36e-13 63.5
MS.gene21936.t1 MTR_6g466130 40.187 107 51 3 6 104 46 147 5.49e-13 64.7
MS.gene21936.t1 MTR_1g006660 47.826 92 31 4 13 97 40 121 6.14e-13 65.1
MS.gene21936.t1 MTR_6g088425 50.575 87 38 3 18 104 49 130 6.45e-13 64.7
MS.gene21936.t1 MTR_8g092460 48.485 66 32 1 12 77 139 202 7.13e-13 65.1
MS.gene21936.t1 MTR_4g086220 50.847 59 27 1 18 76 96 152 1.43e-12 63.5
MS.gene21936.t1 MTR_4g008860 47.541 61 30 1 16 76 6 64 1.71e-12 62.8
MS.gene21936.t1 MTR_2g019750 46.667 60 32 0 17 76 49 108 1.94e-12 63.9
MS.gene21936.t1 MTR_4g088005 44.776 67 34 2 16 81 55 119 2.21e-12 60.8
MS.gene21936.t1 MTR_4g078710 44.595 74 35 2 11 80 17 88 2.71e-12 62.8
MS.gene21936.t1 MTR_1g074310 52.459 61 26 2 17 76 20 78 2.80e-12 61.2
MS.gene21936.t1 MTR_2g014340 49.153 59 28 1 18 76 19 75 3.14e-12 62.0
MS.gene21936.t1 MTR_4g086160 50.847 59 27 1 18 76 96 152 3.30e-12 62.8
MS.gene21936.t1 MTR_3g053690 40.741 81 40 2 2 76 5 83 3.70e-12 62.4
MS.gene21936.t1 MTR_4g086190 50.847 59 27 1 18 76 96 152 3.77e-12 62.4
MS.gene21936.t1 MTR_4g119270 45.070 71 33 2 17 81 52 122 4.02e-12 63.2
MS.gene21936.t1 MTR_6g465430 50.000 60 28 1 18 77 54 111 5.84e-12 62.0
MS.gene21936.t1 MTR_5g082950 59.184 49 19 1 13 60 60 108 6.22e-12 62.8
MS.gene21936.t1 MTR_2g078680 40.000 80 43 2 17 93 30 107 7.23e-12 61.2
MS.gene21936.t1 MTR_3g106290 49.206 63 26 2 16 76 6 64 7.80e-12 60.8
MS.gene21936.t1 MTR_6g465460 40.230 87 47 2 18 104 54 135 9.55e-12 61.6
MS.gene21936.t1 MTR_2g435590 48.333 60 29 1 18 77 96 153 1.60e-11 60.8
MS.gene21936.t1 MTR_4g054740 28.472 144 86 6 12 143 91 229 1.69e-11 60.8
MS.gene21936.t1 MTR_6g465530 50.000 60 28 1 18 77 33 90 1.90e-11 60.1
MS.gene21936.t1 MTR_1g097190 55.814 43 19 0 18 60 23 65 2.17e-11 58.9
MS.gene21936.t1 MTR_6g465850 40.230 87 47 2 18 104 63 144 3.10e-11 60.5
MS.gene21936.t1 MTR_4g054360 44.776 67 34 2 12 77 118 182 3.51e-11 60.1
MS.gene21936.t1 MTR_8g069360 40.984 61 36 0 17 77 43 103 3.61e-11 60.8
MS.gene21936.t1 MTR_8g099215 57.778 45 19 0 16 60 105 149 3.69e-11 60.5
MS.gene21936.t1 MTR_1g074230 40.000 75 42 2 4 77 5 77 4.21e-11 57.8
MS.gene21936.t1 MTR_6g465510 48.333 60 29 1 18 77 33 90 6.27e-11 58.9
MS.gene21936.t1 MTR_1g048610 40.789 76 42 2 2 76 73 146 7.15e-11 58.9
MS.gene21936.t1 MTR_5g075570 50.980 51 25 0 8 58 114 164 7.85e-11 59.3
MS.gene21936.t1 MTR_7g084370 56.818 44 19 0 17 60 41 84 9.88e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21936.t1 AT1G22810 43.590 156 65 2 10 165 6 138 3.88e-42 137
MS.gene21936.t1 AT1G71520 43.750 160 59 4 10 165 6 138 1.82e-40 133
MS.gene21936.t1 AT5G67190 46.535 101 50 2 15 115 18 114 2.30e-25 96.3
MS.gene21936.t1 AT3G50260 50.000 94 41 3 16 109 19 106 7.46e-25 94.0
MS.gene21936.t1 AT2G23340 46.392 97 47 2 16 109 26 120 7.72e-25 94.7
MS.gene21936.t1 AT1G21910 45.794 107 52 2 18 120 50 154 1.66e-24 95.1
MS.gene21936.t1 AT1G46768 40.909 132 69 3 14 142 27 152 4.61e-24 92.0
MS.gene21936.t1 AT1G46768 40.909 132 69 3 14 142 27 152 4.61e-24 92.0
MS.gene21936.t1 AT1G46768 45.045 111 47 4 16 119 29 132 4.98e-23 89.0
MS.gene21936.t1 AT1G19210 63.934 61 20 1 17 77 11 69 5.86e-23 90.1
MS.gene21936.t1 AT4G36900 42.056 107 53 2 15 114 27 131 3.11e-22 88.6
MS.gene21936.t1 AT5G25810 41.228 114 51 3 2 104 9 117 1.33e-21 87.4
MS.gene21936.t1 AT1G74930 46.591 88 42 2 17 104 20 102 1.65e-21 86.7
MS.gene21936.t1 AT4G06746 44.898 98 50 2 14 109 32 127 3.20e-21 84.7
MS.gene21936.t1 AT5G21960 36.441 118 70 2 13 130 2 114 3.48e-21 86.3
MS.gene21936.t1 AT4G31060 41.284 109 60 2 12 120 21 125 1.09e-20 84.3
MS.gene21936.t1 AT1G71450 56.250 64 26 1 18 81 24 85 1.49e-20 84.0
MS.gene21936.t1 AT5G11590 49.425 87 39 2 18 104 51 132 7.36e-20 83.2
MS.gene21936.t1 AT1G01250 44.444 90 45 2 8 97 35 119 1.89e-19 81.3
MS.gene21936.t1 AT4G32800 43.925 107 52 4 5 108 4 105 2.81e-19 81.3
MS.gene21936.t1 AT1G77640 50.000 92 41 2 16 104 41 130 1.19e-18 80.1
MS.gene21936.t1 AT4G16750 59.016 61 23 1 18 78 40 98 2.41e-18 78.2
MS.gene21936.t1 AT2G44940 45.745 94 46 2 13 106 95 183 4.97e-18 79.3
MS.gene21936.t1 AT2G22200 50.685 73 33 2 7 79 61 130 2.55e-17 77.0
MS.gene21936.t1 AT3G60490 46.739 92 42 3 8 97 59 145 2.65e-17 76.6
MS.gene21936.t1 AT2G35700 38.281 128 67 5 14 135 41 162 3.30e-17 75.5
MS.gene21936.t1 AT4G13620 37.097 124 70 4 16 135 230 349 5.24e-17 77.4
MS.gene21936.t1 AT1G22190 48.611 72 35 1 7 78 72 141 5.82e-17 75.9
MS.gene21936.t1 AT1G44830 52.273 88 37 2 16 99 33 119 1.10e-16 74.3
MS.gene21936.t1 AT4G27950 37.069 116 58 3 9 119 109 214 1.20e-16 75.9
MS.gene21936.t1 AT4G39780 52.381 63 28 1 16 78 91 151 1.58e-16 75.1
MS.gene21936.t1 AT3G16280 47.500 80 37 2 18 97 61 135 3.60e-16 73.6
MS.gene21936.t1 AT3G16280 47.500 80 37 2 18 97 79 153 4.16e-16 73.6
MS.gene21936.t1 AT5G65130 43.023 86 44 2 16 101 111 191 6.13e-16 73.6
MS.gene21936.t1 AT5G65130 43.023 86 44 2 16 101 109 189 6.37e-16 73.2
MS.gene21936.t1 AT5G25390 45.455 88 44 2 14 99 3 88 7.03e-16 71.6
MS.gene21936.t1 AT2G36450 50.649 77 32 3 9 81 3 77 1.36e-15 70.9
MS.gene21936.t1 AT1G78080 52.381 63 28 1 16 78 150 210 1.48e-15 72.8
MS.gene21936.t1 AT2G25820 54.098 61 26 1 18 78 26 84 2.19e-15 71.2
MS.gene21936.t1 AT1G22985 47.368 76 37 1 14 86 71 146 2.25e-15 69.7
MS.gene21936.t1 AT1G33760 45.652 92 45 2 14 105 16 102 5.39e-15 69.3
MS.gene21936.t1 AT1G36060 52.381 63 28 1 16 78 141 201 6.71e-15 70.9
MS.gene21936.t1 AT5G61890 41.860 86 43 2 16 96 88 171 6.97e-15 70.1
MS.gene21936.t1 AT2G44840 51.515 66 29 2 17 81 91 154 1.06e-14 69.3
MS.gene21936.t1 AT5G51990 44.318 88 44 2 18 105 54 136 1.13e-14 69.3
MS.gene21936.t1 AT1G75490 49.296 71 34 1 12 82 36 104 1.97e-14 68.2
MS.gene21936.t1 AT3G61630 32.857 140 79 4 16 140 103 242 2.60e-14 69.3
MS.gene21936.t1 AT3G11020 48.571 70 34 1 12 81 72 139 3.42e-14 68.9
MS.gene21936.t1 AT5G13330 51.613 62 28 1 16 77 37 96 3.59e-14 67.8
MS.gene21936.t1 AT5G05410 30.769 169 90 7 18 164 79 242 4.55e-14 68.6
MS.gene21936.t1 AT2G20880 48.438 64 31 1 16 79 185 246 5.39e-14 68.6
MS.gene21936.t1 AT4G11140 50.820 61 28 1 16 76 85 143 5.82e-14 68.2
MS.gene21936.t1 AT4G23750 49.296 71 33 1 4 74 111 178 5.85e-14 68.6
MS.gene21936.t1 AT4G23750 49.296 71 33 1 4 74 111 178 5.85e-14 68.6
MS.gene21936.t1 AT1G12610 37.864 103 59 2 18 120 30 127 6.19e-14 67.0
MS.gene21936.t1 AT4G28140 51.613 62 28 1 16 77 141 200 6.83e-14 67.8
MS.gene21936.t1 AT5G05410 30.769 169 90 7 18 164 79 242 8.79e-14 67.8
MS.gene21936.t1 AT5G53290 54.545 55 25 0 16 70 123 177 1.01e-13 67.8
MS.gene21936.t1 AT3G23230 50.746 67 30 2 12 77 14 78 1.01e-13 65.1
MS.gene21936.t1 AT2G33710 43.478 69 37 1 11 79 63 129 1.99e-13 65.9
MS.gene21936.t1 AT2G33710 43.478 69 37 1 11 79 63 129 2.10e-13 65.9
MS.gene21936.t1 AT4G25470 39.583 96 41 3 18 107 51 135 2.24e-13 65.5
MS.gene21936.t1 AT4G25480 53.333 60 26 1 18 77 51 108 2.84e-13 65.5
MS.gene21936.t1 AT1G77200 52.500 80 33 3 18 97 43 117 2.91e-13 65.9
MS.gene21936.t1 AT3G16770 49.180 61 29 1 18 78 79 137 3.23e-13 65.5
MS.gene21936.t1 AT1G15360 50.820 61 28 1 16 76 5 63 3.72e-13 64.7
MS.gene21936.t1 AT1G15360 50.820 61 28 1 16 76 5 63 3.72e-13 64.7
MS.gene21936.t1 AT5G25390 44.318 88 42 3 14 99 3 85 5.45e-13 63.9
MS.gene21936.t1 AT4G25490 53.333 60 26 1 18 77 48 105 8.81e-13 63.9
MS.gene21936.t1 AT5G25190 49.180 61 29 1 16 76 6 64 8.88e-13 63.5
MS.gene21936.t1 AT1G72360 33.962 106 62 2 3 108 10 107 9.21e-13 63.9
MS.gene21936.t1 AT1G12890 61.364 44 17 0 17 60 20 63 1.21e-12 63.5
MS.gene21936.t1 AT2G40340 47.541 61 30 1 18 78 14 72 1.47e-12 64.3
MS.gene21936.t1 AT2G40340 47.541 61 30 1 18 78 14 72 1.47e-12 64.3
MS.gene21936.t1 AT2G40340 47.541 61 30 1 18 78 14 72 1.47e-12 64.3
MS.gene21936.t1 AT1G28370 46.296 54 29 0 5 58 7 60 1.55e-12 62.4
MS.gene21936.t1 AT2G47520 48.333 60 29 1 18 77 50 107 1.56e-12 62.4
MS.gene21936.t1 AT1G28370 46.296 54 29 0 5 58 45 98 1.65e-12 63.2
MS.gene21936.t1 AT1G28360 44.444 72 38 1 9 80 3 72 2.09e-12 62.4
MS.gene21936.t1 AT3G15210 42.105 76 40 2 6 79 11 84 2.09e-12 63.2
MS.gene21936.t1 AT3G57600 50.000 62 29 1 17 78 27 86 2.32e-12 63.5
MS.gene21936.t1 AT1G63030 36.893 103 60 2 18 120 30 127 2.75e-12 62.0
MS.gene21936.t1 AT1G63030 36.893 103 60 2 18 120 30 127 2.75e-12 62.0
MS.gene21936.t1 AT2G40340 47.541 61 30 1 18 78 72 130 3.38e-12 63.2
MS.gene21936.t1 AT2G40340 47.541 61 30 1 18 78 72 130 3.44e-12 63.5
MS.gene21936.t1 AT2G40340 47.541 61 30 1 18 78 79 137 3.69e-12 63.5
MS.gene21936.t1 AT5G13910 42.857 77 40 2 1 75 1 75 3.79e-12 62.0
MS.gene21936.t1 AT1G71130 46.970 66 30 1 16 76 77 142 4.52e-12 61.2
MS.gene21936.t1 AT1G72360 35.849 106 57 3 6 108 61 158 4.68e-12 62.8
MS.gene21936.t1 AT1G72360 36.538 104 55 3 8 108 61 156 6.67e-12 62.0
MS.gene21936.t1 AT2G46310 56.818 44 19 0 17 60 98 141 7.49e-12 62.4
MS.gene21936.t1 AT1G28160 35.632 87 53 1 17 103 38 121 1.37e-11 61.2
MS.gene21936.t1 AT5G43410 49.180 61 28 2 17 76 14 72 1.40e-11 58.9
MS.gene21936.t1 AT5G44210 42.254 71 39 1 6 76 21 89 1.70e-11 60.1
MS.gene21936.t1 AT1G12980 36.986 73 46 0 4 76 43 115 2.10e-11 61.2
MS.gene21936.t1 AT5G11190 42.222 90 46 2 14 99 3 90 2.31e-11 59.7
MS.gene21936.t1 AT1G43160 39.286 84 49 1 8 91 51 132 4.04e-11 58.9
MS.gene21936.t1 AT5G47220 46.774 62 30 2 16 76 115 174 4.19e-11 59.7
MS.gene21936.t1 AT5G18560 47.458 59 29 1 17 75 54 110 4.34e-11 60.5
MS.gene21936.t1 AT2G40350 47.458 59 29 1 18 76 74 130 6.48e-11 58.2
MS.gene21936.t1 AT5G18450 60.465 43 17 0 18 60 34 76 7.41e-11 59.7
MS.gene21936.t1 AT2G40350 47.458 59 29 1 18 76 67 123 8.34e-11 57.8

Find 29 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGGAACTCAAGAACGTTTA+TGG 0.162126 5.1:+2796860 MS.gene21936:CDS
TCCATGTGATGATTCCTTTC+TGG 0.201142 5.1:-2797111 None:intergenic
TGTGCCACTGCAGCAGCTTC+TGG 0.288160 5.1:-2796901 None:intergenic
TTTAAAGAGACCTTCTACTT+TGG 0.299862 5.1:+2796942 MS.gene21936:CDS
CATAAACGTTCTTGAGTTCC+TGG 0.344488 5.1:-2796859 None:intergenic
ATTATACATTGCAGATGAAT+TGG 0.359036 5.1:+2796740 None:intergenic
TGTTCAAATCCTCTTGTTGT+TGG 0.378910 5.1:-2797206 None:intergenic
GGCGTAGAAAGTGGGGAAAA+TGG 0.392698 5.1:+2796818 MS.gene21936:CDS
CAATAAGAAGACAAAATATC+AGG 0.407244 5.1:-2796979 None:intergenic
GTATCAGAGATTCGTGTTCC+AGG 0.411811 5.1:+2796841 MS.gene21936:CDS
TTGAGTTTGTCCAAAGTAGA+AGG 0.430828 5.1:-2796952 None:intergenic
ACAAGAAGTTCAAGGGAGTT+AGG 0.435201 5.1:+2796797 MS.gene21936:CDS
GTCTCCTAGGTCTGTTCAAA+AGG 0.440851 5.1:+2797026 MS.gene21936:CDS
GCGTAGAAAGTGGGGAAAAT+GGG 0.446577 5.1:+2796819 MS.gene21936:CDS
GAGATAATTTGATGTCTCCT+AGG 0.471023 5.1:+2797013 MS.gene21936:CDS
AACTCAAGAACGTTTATGGT+TGG 0.473152 5.1:+2796864 MS.gene21936:CDS
AAGCAACAACTTTGCCAGAA+AGG 0.482224 5.1:+2797097 MS.gene21936:CDS
ACTCAAGAACGTTTATGGTT+GGG 0.486534 5.1:+2796865 MS.gene21936:CDS
AAATTATAGATAATCTTCAA+TGG 0.511121 5.1:-2797232 None:intergenic
TGACAACAACAAGAAGTTCA+AGG 0.541294 5.1:+2796789 MS.gene21936:CDS
AGGGAGTTAGGCGTAGAAAG+TGG 0.541599 5.1:+2796809 MS.gene21936:CDS
GAAGGTTTGGGTGATGATCA+AGG 0.545874 5.1:+2797165 MS.gene21936:CDS
GGGAGTTAGGCGTAGAAAGT+GGG 0.554439 5.1:+2796810 MS.gene21936:CDS
AGTTTCTAGCCAACAACAAG+AGG 0.563778 5.1:+2797197 MS.gene21936:CDS
GGTTGCTTCAGATGTTGCTA+TGG 0.573939 5.1:+2797047 MS.gene21936:CDS
GACAACAACAAGAAGTTCAA+GGG 0.587791 5.1:+2796790 MS.gene21936:CDS
TACACCAGAAGCTGCTGCAG+TGG 0.594266 5.1:+2796897 MS.gene21936:CDS
GGAGTTAGGCGTAGAAAGTG+GGG 0.655612 5.1:+2796811 MS.gene21936:CDS
GCCAGAAAGGAATCATCACA+TGG 0.697762 5.1:+2797110 MS.gene21936:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CAATAAGAAGACAAAATATC+AGG - chr5.1:2796982-2797001 None:intergenic 25.0%
TTTAAAGAGACCTTCTACTT+TGG + chr5.1:2796942-2796961 MS.gene21936:CDS 30.0%
!!! TTGTTGCTGATGATGTTTTT+TGG + chr5.1:2797139-2797158 MS.gene21936:CDS 30.0%
AACTCAAGAACGTTTATGGT+TGG + chr5.1:2796864-2796883 MS.gene21936:CDS 35.0%
ACTCAAGAACGTTTATGGTT+GGG + chr5.1:2796865-2796884 MS.gene21936:CDS 35.0%
GACAACAACAAGAAGTTCAA+GGG + chr5.1:2796790-2796809 MS.gene21936:CDS 35.0%
GAGATAATTTGATGTCTCCT+AGG + chr5.1:2797013-2797032 MS.gene21936:CDS 35.0%
TGACAACAACAAGAAGTTCA+AGG + chr5.1:2796789-2796808 MS.gene21936:CDS 35.0%
TTGAGTTTGTCCAAAGTAGA+AGG - chr5.1:2796955-2796974 None:intergenic 35.0%
! TGTTCAAATCCTCTTGTTGT+TGG - chr5.1:2797209-2797228 None:intergenic 35.0%
!!! TGCTGATGATGTTTTTTGGT+GGG + chr5.1:2797143-2797162 MS.gene21936:CDS 35.0%
!!! TTGCTGATGATGTTTTTTGG+TGG + chr5.1:2797142-2797161 MS.gene21936:CDS 35.0%
AAGCAACAACTTTGCCAGAA+AGG + chr5.1:2797097-2797116 MS.gene21936:CDS 40.0%
ACAAGAAGTTCAAGGGAGTT+AGG + chr5.1:2796797-2796816 MS.gene21936:CDS 40.0%
CAGGAACTCAAGAACGTTTA+TGG + chr5.1:2796860-2796879 MS.gene21936:CDS 40.0%
CATAAACGTTCTTGAGTTCC+TGG - chr5.1:2796862-2796881 None:intergenic 40.0%
TCCATGTGATGATTCCTTTC+TGG - chr5.1:2797114-2797133 None:intergenic 40.0%
!! AGTTTCTAGCCAACAACAAG+AGG + chr5.1:2797197-2797216 MS.gene21936:CDS 40.0%
!!! GATGATGTTTTTTGGTGGGA+AGG + chr5.1:2797147-2797166 MS.gene21936:CDS 40.0%
!!! GTTTTTTGGTGGGAAGGTTT+GGG + chr5.1:2797153-2797172 MS.gene21936:CDS 40.0%
!!! TGTTTTTTGGTGGGAAGGTT+TGG + chr5.1:2797152-2797171 MS.gene21936:CDS 40.0%
GAAGGTTTGGGTGATGATCA+AGG + chr5.1:2797165-2797184 MS.gene21936:CDS 45.0%
GCCAGAAAGGAATCATCACA+TGG + chr5.1:2797110-2797129 MS.gene21936:CDS 45.0%
GCGTAGAAAGTGGGGAAAAT+GGG + chr5.1:2796819-2796838 MS.gene21936:CDS 45.0%
GGTTGCTTCAGATGTTGCTA+TGG + chr5.1:2797047-2797066 MS.gene21936:CDS 45.0%
GTATCAGAGATTCGTGTTCC+AGG + chr5.1:2796841-2796860 MS.gene21936:CDS 45.0%
GTCTCCTAGGTCTGTTCAAA+AGG + chr5.1:2797026-2797045 MS.gene21936:CDS 45.0%
! GCAACCTTTTGAACAGACCT+AGG - chr5.1:2797033-2797052 None:intergenic 45.0%
AGGGAGTTAGGCGTAGAAAG+TGG + chr5.1:2796809-2796828 MS.gene21936:CDS 50.0%
GGAGTTAGGCGTAGAAAGTG+GGG + chr5.1:2796811-2796830 MS.gene21936:CDS 50.0%
GGCGTAGAAAGTGGGGAAAA+TGG + chr5.1:2796818-2796837 MS.gene21936:CDS 50.0%
GGGAGTTAGGCGTAGAAAGT+GGG + chr5.1:2796810-2796829 MS.gene21936:CDS 50.0%
TACACCAGAAGCTGCTGCAG+TGG + chr5.1:2796897-2796916 MS.gene21936:CDS 55.0%
! TGTGCCACTGCAGCAGCTTC+TGG - chr5.1:2796904-2796923 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 2796754 2797251 2796754 ID=MS.gene21936
chr5.1 mRNA 2796754 2797251 2796754 ID=MS.gene21936.t1;Parent=MS.gene21936
chr5.1 exon 2796754 2797251 2796754 ID=MS.gene21936.t1.exon1;Parent=MS.gene21936.t1
chr5.1 CDS 2796754 2797251 2796754 ID=cds.MS.gene21936.t1;Parent=MS.gene21936.t1
Gene Sequence

>MS.gene21936

ATGAATTGGTCTACTTCAACAACAAGCACAAGTGATGACAACAACAAGAAGTTCAAGGGAGTTAGGCGTAGAAAGTGGGGAAAATGGGTATCAGAGATTCGTGTTCCAGGAACTCAAGAACGTTTATGGTTGGGAACTTATGCTACACCAGAAGCTGCTGCAGTGGCACACGATGTAGCTTTTTATTGTTTAAAGAGACCTTCTACTTTGGACAAACTCAACTTTCCTGATATTTTGTCTTCTTATTGTATTCAACAAAGAGATAATTTGATGTCTCCTAGGTCTGTTCAAAAGGTTGCTTCAGATGTTGCTATGGATGTTGATGCACGAAACATTGCAAGTCAAGCAACAACTTTGCCAGAAAGGAATCATCACATGGACAATGTTGTTGCTGATGATGTTTTTTGGTGGGAAGGTTTGGGTGATGATCAAGGAGTTACTCAAGTTTCTAGCCAACAACAAGAGGATTTGAACATTTCCATTGAAGATTATCTATAA
Protein sequence

>MS.gene21936.t1

MNWSTSTTSTSDDNNKKFKGVRRRKWGKWVSEIRVPGTQERLWLGTYATPEAAAVAHDVAFYCLKRPSTLDKLNFPDILSSYCIQQRDNLMSPRSVQKVASDVAMDVDARNIASQATTLPERNHHMDNVVADDVFWWEGLGDDQGVTQVSSQQQEDLNISIEDYL