Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22267.t1 | RHN66718.1 | 92.8 | 334 | 24 | 0 | 1 | 334 | 110 | 443 | 2.10E-163 | 585.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22267.t1 | A0A396IPR0 | 92.8 | 334 | 24 | 0 | 1 | 334 | 110 | 443 | 1.5e-163 | 585.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049531 | MS.gene22267 | 0.804202 | 2.42E-49 | -1.69E-46 |
| MS.gene050221 | MS.gene22267 | 0.803477 | 3.42E-49 | -1.69E-46 |
| MS.gene051800 | MS.gene22267 | 0.8297 | 4.45E-55 | -1.69E-46 |
| MS.gene054467 | MS.gene22267 | 0.817314 | 3.52E-52 | -1.69E-46 |
| MS.gene054641 | MS.gene22267 | 0.80165 | 8.14E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene22267 | 0.800714 | 1.27E-48 | -1.69E-46 |
| MS.gene05897 | MS.gene22267 | 0.804611 | 1.99E-49 | -1.69E-46 |
| MS.gene059272 | MS.gene22267 | 0.817832 | 2.69E-52 | -1.69E-46 |
| MS.gene06052 | MS.gene22267 | 0.82799 | 1.15E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22267.t1 | MTR_5g089720 | 59.366 | 347 | 112 | 8 | 1 | 334 | 107 | 437 | 2.10e-117 | 346 |
| MS.gene22267.t1 | MTR_5g094340 | 46.154 | 130 | 60 | 4 | 1 | 123 | 128 | 254 | 9.30e-25 | 103 |
| MS.gene22267.t1 | MTR_5g094340 | 47.674 | 86 | 38 | 2 | 249 | 334 | 339 | 417 | 1.60e-15 | 77.4 |
| MS.gene22267.t1 | MTR_3g055960 | 45.977 | 87 | 39 | 2 | 248 | 334 | 354 | 432 | 2.63e-14 | 73.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22267.t1 | AT5G12300 | 62.097 | 124 | 36 | 3 | 1 | 118 | 96 | 214 | 2.06e-40 | 145 |
| MS.gene22267.t1 | AT1G50570 | 33.333 | 351 | 144 | 9 | 1 | 334 | 111 | 388 | 3.71e-36 | 134 |
| MS.gene22267.t1 | AT1G50570 | 33.333 | 351 | 144 | 9 | 1 | 334 | 111 | 388 | 3.71e-36 | 134 |
| MS.gene22267.t1 | AT5G55530 | 54.622 | 119 | 45 | 3 | 1 | 113 | 123 | 238 | 1.63e-28 | 114 |
| MS.gene22267.t1 | AT5G55530 | 54.622 | 119 | 45 | 3 | 1 | 113 | 123 | 238 | 1.63e-28 | 114 |
| MS.gene22267.t1 | AT5G55530 | 54.622 | 119 | 45 | 3 | 1 | 113 | 123 | 238 | 1.63e-28 | 114 |
Find 39 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCTTATCGGTCTCTAATT+TGG | 0.168132 | 3.4:-38166037 | None:intergenic |
| TCCTTGGAAGTTGGAGTATC+TGG | 0.194020 | 3.4:-38165721 | None:intergenic |
| GGTTTGTGAGTACTTCAATT+TGG | 0.266850 | 3.4:+38165420 | MS.gene22267:CDS |
| TACTTCAATTTGGAATCTTA+TGG | 0.269431 | 3.4:+38165430 | MS.gene22267:CDS |
| GCATCAATTCACCGAGTCTT+TGG | 0.292100 | 3.4:+38165933 | MS.gene22267:CDS |
| AATCTTATGGTTCTGTTTCA+AGG | 0.305198 | 3.4:+38165443 | MS.gene22267:CDS |
| TTGCCGTTTCTTCAACTTAG+TGG | 0.324790 | 3.4:+38165484 | MS.gene22267:CDS |
| TCATTAGCCACATTGATATC+AGG | 0.338851 | 3.4:-38165385 | None:intergenic |
| GAACTCAAAGTCGAATTGAT+TGG | 0.346252 | 3.4:-38165280 | None:intergenic |
| GTTTCAAGGCCTAATTCAAT+AGG | 0.354760 | 3.4:+38165457 | MS.gene22267:CDS |
| TCTAGTTTCGCTTTCTTCCT+TGG | 0.365371 | 3.4:-38165737 | None:intergenic |
| GATTATCATGATTGTGATCA+AGG | 0.365568 | 3.4:-38165978 | None:intergenic |
| GTGAGATTTGGATGTTTAGT+AGG | 0.380572 | 3.4:+38165092 | None:intergenic |
| GGCAACGATCCTATTGAATT+AGG | 0.380609 | 3.4:-38165466 | None:intergenic |
| GGCTAATGAGAATCAGAAAA+TGG | 0.381354 | 3.4:+38165399 | MS.gene22267:CDS |
| AGAATCATGAGTCCATTTCT+AGG | 0.384976 | 3.4:+38165554 | MS.gene22267:CDS |
| AATATAGAAGCTGCAAAATT+CGG | 0.391192 | 3.4:+38165817 | MS.gene22267:CDS |
| TCAATTCTAGAATACTCAAT+TGG | 0.410165 | 3.4:-38165358 | None:intergenic |
| TCCGCCACAAAATATCGATA+TGG | 0.421403 | 3.4:+38165855 | MS.gene22267:CDS |
| AGAGCTTGAAAACCTAGAAA+TGG | 0.425833 | 3.4:-38165566 | None:intergenic |
| TTTGAGTTCGAAACCACAAT+CGG | 0.438281 | 3.4:+38165294 | MS.gene22267:CDS |
| AAAACGAGACGAATATTGAT+AGG | 0.461835 | 3.4:+38165794 | MS.gene22267:CDS |
| TCGCTTTCTTCCTTGGAAGT+TGG | 0.466636 | 3.4:-38165730 | None:intergenic |
| AATCATGATAATCAAAATCA+AGG | 0.499360 | 3.4:+38165988 | MS.gene22267:CDS |
| TGATGTAATTCAAAATCATA+AGG | 0.500874 | 3.4:+38166011 | MS.gene22267:CDS |
| GTTAATTGAACAGTTCCAGC+TGG | 0.526635 | 3.4:-38165235 | None:intergenic |
| GAACCACTAAGTTGAAGAAA+CGG | 0.536520 | 3.4:-38165487 | None:intergenic |
| GAAATGACAATGAATTCGTC+AGG | 0.546995 | 3.4:+38165529 | MS.gene22267:CDS |
| TCCAGATACTCCAACTTCCA+AGG | 0.556108 | 3.4:+38165720 | MS.gene22267:CDS |
| TTGAGTTCGAAACCACAATC+GGG | 0.560282 | 3.4:+38165295 | MS.gene22267:CDS |
| TGATTGTGATCAAGGTCCAT+TGG | 0.563994 | 3.4:-38165970 | None:intergenic |
| ATTTCACAGTCAACATCTTG+AGG | 0.577410 | 3.4:-38165511 | None:intergenic |
| GATGAATTAGTACCCGATTG+TGG | 0.591123 | 3.4:-38165307 | None:intergenic |
| GGCGAAAATGAAGCTACCAA+TGG | 0.599527 | 3.4:+38165954 | MS.gene22267:CDS |
| GCCACAAAATATCGATATGG+AGG | 0.626756 | 3.4:+38165858 | MS.gene22267:CDS |
| GGAGTTAAAGTTCTCAACCA+AGG | 0.638594 | 3.4:+38165771 | MS.gene22267:CDS |
| TGAATTTCCTGATATCAATG+TGG | 0.679366 | 3.4:+38165378 | MS.gene22267:CDS |
| AGCGATGATAGAAACACGAA+CGG | 0.692429 | 3.4:+38165604 | MS.gene22267:CDS |
| GTGATAAAGAGTAATTTGAG+AGG | 0.697973 | 3.4:+38165634 | MS.gene22267:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCATGATAATCAAAATCA+AGG | + | chr3.4:38165988-38166007 | MS.gene22267:CDS | 20.0% |
| !! | TGATGTAATTCAAAATCATA+AGG | + | chr3.4:38166011-38166030 | MS.gene22267:CDS | 20.0% |
| ! | AATATAGAAGCTGCAAAATT+CGG | + | chr3.4:38165817-38165836 | MS.gene22267:CDS | 25.0% |
| ! | TTACCAAAAACTTCAGAAAT+CGG | - | chr3.4:38165163-38165182 | None:intergenic | 25.0% |
| !! | ACTTAGAAGATCAACTTTTA+GGG | + | chr3.4:38165125-38165144 | MS.gene22267:CDS | 25.0% |
| !! | TACTTCAATTTGGAATCTTA+TGG | + | chr3.4:38165430-38165449 | MS.gene22267:CDS | 25.0% |
| !!! | ATTTCTGAAGTTTTTGGTAA+AGG | + | chr3.4:38165163-38165182 | MS.gene22267:CDS | 25.0% |
| !!! | TCAATTCTAGAATACTCAAT+TGG | - | chr3.4:38165361-38165380 | None:intergenic | 25.0% |
| AAAACGAGACGAATATTGAT+AGG | + | chr3.4:38165794-38165813 | MS.gene22267:CDS | 30.0% | |
| ACCGATAAGAAGAAAAATGA+CGG | + | chr3.4:38166048-38166067 | MS.gene22267:CDS | 30.0% | |
| CGAAACTAGAGATGAAAAAA+AGG | + | chr3.4:38165750-38165769 | MS.gene22267:CDS | 30.0% | |
| GATTATCATGATTGTGATCA+AGG | - | chr3.4:38165981-38166000 | None:intergenic | 30.0% | |
| GTGATAAAGAGTAATTTGAG+AGG | + | chr3.4:38165634-38165653 | MS.gene22267:CDS | 30.0% | |
| TGAATTTCCTGATATCAATG+TGG | + | chr3.4:38165378-38165397 | MS.gene22267:CDS | 30.0% | |
| ! | AATCTTATGGTTCTGTTTCA+AGG | + | chr3.4:38165443-38165462 | MS.gene22267:CDS | 30.0% |
| ! | CACTTAGAAGATCAACTTTT+AGG | + | chr3.4:38165124-38165143 | MS.gene22267:CDS | 30.0% |
| AGAATCATGAGTCCATTTCT+AGG | + | chr3.4:38165554-38165573 | MS.gene22267:CDS | 35.0% | |
| AGAGCTTGAAAACCTAGAAA+TGG | - | chr3.4:38165569-38165588 | None:intergenic | 35.0% | |
| ATTTCACAGTCAACATCTTG+AGG | - | chr3.4:38165514-38165533 | None:intergenic | 35.0% | |
| CCGATAAGAAGAAAAATGAC+GGG | + | chr3.4:38166049-38166068 | MS.gene22267:CDS | 35.0% | |
| CTTCTTATCGGTCTCTAATT+TGG | - | chr3.4:38166040-38166059 | None:intergenic | 35.0% | |
| GAAATGACAATGAATTCGTC+AGG | + | chr3.4:38165529-38165548 | MS.gene22267:CDS | 35.0% | |
| GAACTCAAAGTCGAATTGAT+TGG | - | chr3.4:38165283-38165302 | None:intergenic | 35.0% | |
| GGCTAATGAGAATCAGAAAA+TGG | + | chr3.4:38165399-38165418 | MS.gene22267:CDS | 35.0% | |
| GGTTTGTGAGTACTTCAATT+TGG | + | chr3.4:38165420-38165439 | MS.gene22267:CDS | 35.0% | |
| GTTTCAAGGCCTAATTCAAT+AGG | + | chr3.4:38165457-38165476 | MS.gene22267:CDS | 35.0% | |
| TCATTAGCCACATTGATATC+AGG | - | chr3.4:38165388-38165407 | None:intergenic | 35.0% | |
| TGGAGAATGAAAAAGATCAG+TGG | - | chr3.4:38165218-38165237 | None:intergenic | 35.0% | |
| ! | AATATTCGTCTCGTTTTCCT+TGG | - | chr3.4:38165791-38165810 | None:intergenic | 35.0% |
| ! | GAACCACTAAGTTGAAGAAA+CGG | - | chr3.4:38165490-38165509 | None:intergenic | 35.0% |
| ! | TCCATATCGATATTTTGTGG+CGG | - | chr3.4:38165859-38165878 | None:intergenic | 35.0% |
| ! | TTTGAGTTCGAAACCACAAT+CGG | + | chr3.4:38165294-38165313 | MS.gene22267:CDS | 35.0% |
| !!! | CCCGTCATTTTTCTTCTTAT+CGG | - | chr3.4:38166052-38166071 | None:intergenic | 35.0% |
| !!! | GTTCCGATTTCTGAAGTTTT+TGG | + | chr3.4:38165157-38165176 | MS.gene22267:CDS | 35.0% |
| !!! | TCAACTTTTAGGGTTTGCTT+TGG | + | chr3.4:38165135-38165154 | MS.gene22267:CDS | 35.0% |
| AGCGATGATAGAAACACGAA+CGG | + | chr3.4:38165604-38165623 | MS.gene22267:CDS | 40.0% | |
| GATGAATTAGTACCCGATTG+TGG | - | chr3.4:38165310-38165329 | None:intergenic | 40.0% | |
| GCCACAAAATATCGATATGG+AGG | + | chr3.4:38165858-38165877 | MS.gene22267:CDS | 40.0% | |
| GGAGTTAAAGTTCTCAACCA+AGG | + | chr3.4:38165771-38165790 | MS.gene22267:CDS | 40.0% | |
| GGCAACGATCCTATTGAATT+AGG | - | chr3.4:38165469-38165488 | None:intergenic | 40.0% | |
| GTTAATTGAACAGTTCCAGC+TGG | - | chr3.4:38165238-38165257 | None:intergenic | 40.0% | |
| TCCGCCACAAAATATCGATA+TGG | + | chr3.4:38165855-38165874 | MS.gene22267:CDS | 40.0% | |
| TGATTGTGATCAAGGTCCAT+TGG | - | chr3.4:38165973-38165992 | None:intergenic | 40.0% | |
| TTGAGTTCGAAACCACAATC+GGG | + | chr3.4:38165295-38165314 | MS.gene22267:CDS | 40.0% | |
| ! | CTTCATTTTCGCCAAAGACT+CGG | - | chr3.4:38165947-38165966 | None:intergenic | 40.0% |
| ! | GCCTCCATATCGATATTTTG+TGG | - | chr3.4:38165862-38165881 | None:intergenic | 40.0% |
| ! | TCTAGTTTCGCTTTCTTCCT+TGG | - | chr3.4:38165740-38165759 | None:intergenic | 40.0% |
| ! | TTGCCGTTTCTTCAACTTAG+TGG | + | chr3.4:38165484-38165503 | MS.gene22267:CDS | 40.0% |
| !! | GATCTTTTTCATTCTCCAGC+TGG | + | chr3.4:38165220-38165239 | MS.gene22267:CDS | 40.0% |
| GCATCAATTCACCGAGTCTT+TGG | + | chr3.4:38165933-38165952 | MS.gene22267:CDS | 45.0% | |
| GGCGAAAATGAAGCTACCAA+TGG | + | chr3.4:38165954-38165973 | MS.gene22267:CDS | 45.0% | |
| TCCAGATACTCCAACTTCCA+AGG | + | chr3.4:38165720-38165739 | MS.gene22267:CDS | 45.0% | |
| TCGCTTTCTTCCTTGGAAGT+TGG | - | chr3.4:38165733-38165752 | None:intergenic | 45.0% | |
| ! | TCCTTGGAAGTTGGAGTATC+TGG | - | chr3.4:38165724-38165743 | None:intergenic | 45.0% |
| ! | GACGGGTCGCGCGTGTTTTA+TGG | + | chr3.4:38166066-38166085 | MS.gene22267:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 38165103 | 38166107 | 38165103 | ID=MS.gene22267 |
| chr3.4 | mRNA | 38165103 | 38166107 | 38165103 | ID=MS.gene22267.t1;Parent=MS.gene22267 |
| chr3.4 | exon | 38165103 | 38166107 | 38165103 | ID=MS.gene22267.t1.exon1;Parent=MS.gene22267.t1 |
| chr3.4 | CDS | 38165103 | 38166107 | 38165103 | ID=cds.MS.gene22267.t1;Parent=MS.gene22267.t1 |
| Gene Sequence |
| Protein sequence |
