AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22562

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22562.t1 MTR_5g011620 81.724 290 49 1 8 297 3 288 1.85e-157 462
MS.gene22562.t1 MTR_5g011620 66.667 150 41 3 277 422 114 258 2.02e-50 181
MS.gene22562.t1 MTR_5g011610 68.919 296 79 3 87 372 11 303 3.02e-135 391
MS.gene22562.t1 MTR_5g011610 64.901 151 42 3 278 422 56 201 6.88e-52 177
MS.gene22562.t1 MTR_5g011680 89.552 201 16 2 229 425 93 292 5.13e-117 343
MS.gene22562.t1 MTR_5g011680 61.301 292 76 7 8 267 3 289 5.12e-103 308
MS.gene22562.t1 MTR_4g101990 46.316 285 113 7 11 265 17 291 4.77e-49 170
MS.gene22562.t1 MTR_4g101990 47.619 210 100 3 209 417 84 284 2.16e-38 141
MS.gene22562.t1 MTR_7g074935 46.014 276 113 8 10 270 28 282 1.37e-47 166
MS.gene22562.t1 MTR_7g074935 51.562 192 84 2 222 412 78 261 2.80e-41 149
MS.gene22562.t1 MTR_1g076710 45.041 242 89 7 51 262 49 276 9.41e-43 153
MS.gene22562.t1 MTR_1g076710 55.172 145 63 2 270 413 126 269 4.07e-32 125
MS.gene22562.t1 MTR_8g036160 44.589 231 79 5 51 254 71 279 6.10e-42 152
MS.gene22562.t1 MTR_8g036160 56.115 139 60 1 267 404 131 269 2.90e-36 136
MS.gene22562.t1 MTR_1g061530 37.445 227 121 4 51 262 65 285 1.24e-37 140
MS.gene22562.t1 MTR_1g061530 43.590 156 85 2 264 416 127 282 5.44e-35 133
MS.gene22562.t1 MTR_8g098390 37.500 240 135 5 49 278 95 329 2.44e-37 139
MS.gene22562.t1 MTR_8g098390 36.646 161 90 2 267 416 155 314 1.75e-27 112
MS.gene22562.t1 MTR_6g061670 41.038 212 113 5 51 253 51 259 1.31e-34 131
MS.gene22562.t1 MTR_6g061670 45.070 142 74 1 276 417 123 260 5.09e-27 110
MS.gene22562.t1 MTR_6g061670 40.553 217 112 6 51 253 51 264 2.33e-34 130
MS.gene22562.t1 MTR_6g061670 45.070 142 74 1 276 417 128 265 4.65e-27 110
MS.gene22562.t1 MTR_5g010330 38.756 209 107 5 35 235 54 249 6.56e-33 126
MS.gene22562.t1 MTR_5g010330 41.667 132 76 1 263 394 119 249 1.81e-26 108
MS.gene22562.t1 MTR_8g031100 38.095 231 111 5 49 259 87 305 3.70e-31 122
MS.gene22562.t1 MTR_8g031100 37.356 174 96 3 263 424 140 312 3.93e-25 105
MS.gene22562.t1 MTR_5g011590 46.108 167 54 4 9 175 3 133 1.44e-28 109
MS.gene22562.t1 MTR_5g011590 55.072 69 29 1 267 335 73 139 4.68e-17 77.8
MS.gene22562.t1 MTR_5g011660 78.261 69 15 0 1 69 1 69 1.67e-28 107
MS.gene22562.t1 MTR_4g098460 50.407 123 55 2 121 238 78 199 3.44e-28 112
MS.gene22562.t1 MTR_4g098460 45.299 117 63 1 278 394 81 196 7.33e-26 106
MS.gene22562.t1 MTR_7g116320 39.500 200 109 4 47 241 70 262 4.34e-27 111
MS.gene22562.t1 MTR_7g116320 47.863 117 58 1 278 394 143 256 3.74e-20 91.7
MS.gene22562.t1 MTR_7g077120 36.036 222 117 5 40 250 81 288 4.75e-27 111
MS.gene22562.t1 MTR_7g077120 38.095 147 91 0 263 409 142 288 2.26e-25 106
MS.gene22562.t1 MTR_5g011600 62.500 96 24 3 332 423 5 92 1.62e-26 103
MS.gene22562.t1 MTR_5g011600 58.065 93 36 1 178 270 5 94 2.80e-25 100
MS.gene22562.t1 MTR_7g116320 39.706 204 110 5 47 245 70 265 3.99e-26 107
MS.gene22562.t1 MTR_7g116320 46.903 113 57 1 278 390 143 252 8.50e-18 83.6
MS.gene22562.t1 MTR_4g098460 48.438 128 55 3 121 238 78 204 7.02e-26 106
MS.gene22562.t1 MTR_4g098460 43.443 122 63 2 278 394 81 201 1.46e-23 100
MS.gene22562.t1 MTR_1g079780 50.877 114 53 2 124 234 109 222 8.11e-26 107
MS.gene22562.t1 MTR_1g079780 43.902 123 64 3 278 398 109 228 1.87e-21 95.1
MS.gene22562.t1 MTR_1g038960 33.193 238 128 6 52 275 86 306 3.71e-24 102
MS.gene22562.t1 MTR_1g038960 43.548 124 65 3 272 393 143 263 6.41e-21 93.6
MS.gene22562.t1 MTR_4g055803 35.294 221 123 5 48 258 57 267 3.94e-24 104
MS.gene22562.t1 MTR_4g055803 36.552 145 89 2 263 404 110 254 2.53e-21 95.9
MS.gene22562.t1 MTR_4g055833 35.025 197 108 5 48 234 54 240 2.50e-22 97.8
MS.gene22562.t1 MTR_4g055833 35.172 145 91 2 263 404 107 251 1.43e-18 87.0
MS.gene22562.t1 MTR_5g014360 35.678 199 96 7 52 234 121 303 3.82e-22 97.8
MS.gene22562.t1 MTR_5g014360 40.171 117 66 2 278 393 190 303 1.48e-17 84.3
MS.gene22562.t1 MTR_4g055813 41.912 136 75 3 127 258 22 157 8.97e-22 95.1
MS.gene22562.t1 MTR_4g055813 38.129 139 83 2 267 404 8 144 6.12e-21 92.8
MS.gene22562.t1 MTR_5g013390 32.461 191 112 5 51 234 81 261 4.00e-19 88.2
MS.gene22562.t1 MTR_5g013390 36.441 118 71 3 277 393 147 261 8.89e-15 75.5
MS.gene22562.t1 MTR_6g060670 34.597 211 110 8 43 243 104 296 4.28e-19 87.8
MS.gene22562.t1 MTR_6g060670 41.667 132 72 4 271 402 170 296 8.37e-18 84.0
MS.gene22562.t1 MTR_3g116100 40.152 132 70 3 135 261 154 281 1.30e-17 84.0
MS.gene22562.t1 MTR_3g116100 41.803 122 64 3 282 400 144 261 3.27e-17 82.8
MS.gene22562.t1 MTR_1g038990 30.612 196 102 6 51 240 82 249 1.35e-16 80.9
MS.gene22562.t1 MTR_1g038990 35.043 117 71 3 278 393 131 243 1.52e-12 68.6
MS.gene22562.t1 MTR_4g055705 31.553 206 125 6 48 237 53 258 5.57e-16 78.6
MS.gene22562.t1 MTR_4g055705 34.247 146 88 3 250 392 114 254 9.69e-15 75.1
MS.gene22562.t1 MTR_3g100470 31.618 136 85 3 122 251 99 232 1.03e-15 77.8
MS.gene22562.t1 MTR_3g100470 29.688 128 89 1 278 404 101 228 4.76e-13 69.7
MS.gene22562.t1 MTR_5g011700 39.416 137 72 5 135 263 10 143 5.17e-15 73.6
MS.gene22562.t1 MTR_5g011700 40.566 106 59 2 289 393 10 112 1.01e-12 67.0
MS.gene22562.t1 MTR_5g041430 29.268 246 127 8 51 284 50 260 5.47e-15 75.9
MS.gene22562.t1 MTR_5g041430 31.293 147 95 4 263 408 97 238 3.77e-12 67.4
MS.gene22562.t1 MTR_3g068035 37.190 121 73 2 124 242 106 225 1.49e-14 74.7
MS.gene22562.t1 MTR_3g068035 34.513 113 71 1 281 393 109 218 7.16e-11 63.5
MS.gene22562.t1 MTR_3g023560 36.054 147 82 5 119 257 57 199 5.68e-14 72.0
MS.gene22562.t1 MTR_3g023560 34.188 117 71 2 280 393 64 177 5.91e-13 68.9
MS.gene22562.t1 MTR_7g080980 37.121 132 79 2 122 251 82 211 1.97e-13 70.5
MS.gene22562.t1 MTR_1g044155 34.959 123 77 2 122 242 151 272 2.99e-12 68.2
MS.gene22562.t1 MTR_5g066020 32.031 128 80 4 127 249 83 208 5.61e-12 65.9
MS.gene22562.t1 MTR_5g066020 28.662 157 106 4 258 410 60 214 6.79e-11 62.8
MS.gene22562.t1 MTR_4g098450 35.338 133 79 3 122 247 101 233 5.33e-11 63.5
MS.gene22562.t1 MTR_8g036060 35.294 136 83 3 124 257 113 245 8.21e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22562.t1 AT4G12080 49.145 234 96 7 51 266 89 317 1.20e-57 193
MS.gene22562.t1 AT4G12080 53.988 163 71 2 263 421 155 317 1.06e-46 164
MS.gene22562.t1 AT5G62260 43.891 221 96 6 49 247 74 288 1.30e-45 162
MS.gene22562.t1 AT5G62260 50.365 137 68 0 270 406 152 288 9.60e-37 139
MS.gene22562.t1 AT5G62260 43.891 221 96 6 49 247 74 288 1.30e-45 162
MS.gene22562.t1 AT5G62260 50.365 137 68 0 270 406 152 288 9.60e-37 139
MS.gene22562.t1 AT4G25320 41.553 219 99 6 51 248 86 296 6.94e-44 158
MS.gene22562.t1 AT4G25320 51.748 143 67 1 261 401 147 289 3.58e-37 140
MS.gene22562.t1 AT5G51590 41.026 234 99 5 42 236 62 295 2.72e-43 157
MS.gene22562.t1 AT5G51590 55.645 124 55 0 271 394 171 294 9.40e-36 136
MS.gene22562.t1 AT5G62260 42.342 222 96 7 49 248 74 285 2.47e-42 151
MS.gene22562.t1 AT5G62260 48.800 125 64 0 270 394 152 276 1.74e-32 124
MS.gene22562.t1 AT2G33620 41.791 201 103 3 49 243 95 287 2.27e-39 145
MS.gene22562.t1 AT2G33620 50.000 144 69 1 262 402 144 287 1.41e-35 134
MS.gene22562.t1 AT2G33620 41.791 201 103 3 49 243 95 287 2.27e-39 145
MS.gene22562.t1 AT2G33620 50.000 144 69 1 262 402 144 287 1.41e-35 134
MS.gene22562.t1 AT2G33620 41.791 201 103 3 49 243 95 287 2.27e-39 145
MS.gene22562.t1 AT2G33620 50.000 144 69 1 262 402 144 287 1.41e-35 134
MS.gene22562.t1 AT2G33620 41.791 201 103 3 49 243 95 287 2.27e-39 145
MS.gene22562.t1 AT2G33620 50.000 144 69 1 262 402 144 287 1.41e-35 134
MS.gene22562.t1 AT4G22770 47.030 202 104 2 220 420 94 293 7.00e-39 143
MS.gene22562.t1 AT4G22770 42.609 230 106 6 49 259 70 292 1.69e-36 136
MS.gene22562.t1 AT4G22770 47.030 202 104 2 220 420 94 293 7.00e-39 143
MS.gene22562.t1 AT4G22770 42.609 230 106 6 49 259 70 292 1.69e-36 136
MS.gene22562.t1 AT4G00200 44.105 229 96 6 41 250 40 255 1.09e-36 137
MS.gene22562.t1 AT4G00200 54.545 143 64 1 276 418 121 262 5.35e-36 135
MS.gene22562.t1 AT4G00200 44.105 229 96 6 41 250 40 255 1.09e-36 137
MS.gene22562.t1 AT4G00200 54.545 143 64 1 276 418 121 262 5.35e-36 135
MS.gene22562.t1 AT2G45850 40.359 223 112 5 49 263 96 305 1.30e-35 134
MS.gene22562.t1 AT2G45850 42.177 147 82 1 263 409 147 290 1.31e-31 124
MS.gene22562.t1 AT2G45850 40.359 223 112 5 49 263 96 305 1.30e-35 134
MS.gene22562.t1 AT2G45850 42.177 147 82 1 263 409 147 290 1.31e-31 124
MS.gene22562.t1 AT2G45850 41.237 194 94 4 49 234 96 277 9.97e-33 125
MS.gene22562.t1 AT2G45850 42.748 131 75 0 263 393 147 277 1.77e-28 113
MS.gene22562.t1 AT1G63470 41.146 192 102 4 51 235 105 292 3.15e-32 126
MS.gene22562.t1 AT1G63470 40.909 132 77 1 263 394 162 292 1.35e-25 107
MS.gene22562.t1 AT4G17950 37.327 217 112 5 51 243 131 347 1.54e-31 125
MS.gene22562.t1 AT4G17950 44.000 150 79 3 278 422 221 370 4.61e-28 115
MS.gene22562.t1 AT3G61310 39.474 228 96 7 51 261 101 303 2.46e-31 123
MS.gene22562.t1 AT3G61310 40.909 132 78 0 263 394 149 280 1.90e-24 103
MS.gene22562.t1 AT5G46640 41.256 223 115 5 27 236 78 297 4.15e-30 120
MS.gene22562.t1 AT5G46640 42.236 161 86 2 262 415 162 322 1.54e-22 99.0
MS.gene22562.t1 AT3G04590 35.683 227 135 4 47 271 101 318 5.90e-29 117
MS.gene22562.t1 AT3G04590 44.828 116 61 1 278 393 171 283 4.75e-23 100
MS.gene22562.t1 AT3G04590 36.410 195 115 2 49 241 103 290 1.13e-28 114
MS.gene22562.t1 AT3G04590 44.828 116 61 1 278 393 171 283 1.45e-23 100
MS.gene22562.t1 AT1G63480 35.366 246 108 8 51 268 93 315 6.83e-28 113
MS.gene22562.t1 AT1G63480 38.356 146 85 2 263 408 139 279 1.03e-24 104
MS.gene22562.t1 AT1G63480 35.366 246 108 8 51 268 93 315 6.83e-28 113
MS.gene22562.t1 AT1G63480 38.356 146 85 2 263 408 139 279 1.03e-24 104
MS.gene22562.t1 AT1G63480 35.366 246 108 8 51 268 93 315 6.83e-28 113
MS.gene22562.t1 AT1G63480 38.356 146 85 2 263 408 139 279 1.03e-24 104
MS.gene22562.t1 AT1G63480 35.366 246 108 8 51 268 93 315 6.83e-28 113
MS.gene22562.t1 AT1G63480 38.356 146 85 2 263 408 139 279 1.03e-24 104
MS.gene22562.t1 AT1G63480 35.366 246 108 8 51 268 93 315 6.83e-28 113
MS.gene22562.t1 AT1G63480 38.356 146 85 2 263 408 139 279 1.03e-24 104
MS.gene22562.t1 AT1G63480 35.366 246 108 8 51 268 99 321 7.82e-28 113
MS.gene22562.t1 AT1G63480 38.356 146 85 2 263 408 145 285 1.21e-24 104
MS.gene22562.t1 AT1G63480 35.366 246 108 8 51 268 99 321 7.82e-28 113
MS.gene22562.t1 AT1G63480 38.356 146 85 2 263 408 145 285 1.21e-24 104
MS.gene22562.t1 AT2G36560 44.167 120 65 2 278 395 105 224 5.97e-21 95.5
MS.gene22562.t1 AT2G36560 35.821 134 76 3 121 244 102 235 1.41e-17 85.5
MS.gene22562.t1 AT5G49700 34.815 135 77 3 128 253 87 219 2.21e-17 82.4
MS.gene22562.t1 AT5G49700 32.353 136 80 4 278 402 83 217 4.84e-14 72.4
MS.gene22562.t1 AT3G60870 33.628 113 71 2 124 234 86 196 8.56e-15 74.7
MS.gene22562.t1 AT3G60870 28.221 163 105 4 231 393 46 196 4.95e-12 66.2
MS.gene22562.t1 AT1G14490 34.848 132 75 3 128 250 34 163 5.17e-14 71.2
MS.gene22562.t1 AT1G14490 30.968 155 94 4 279 422 31 183 8.90e-12 64.7
MS.gene22562.t1 AT1G14490 34.848 132 75 3 128 250 53 182 5.43e-14 71.6
MS.gene22562.t1 AT1G14490 29.814 161 100 4 273 422 44 202 1.30e-11 64.7
MS.gene22562.t1 AT2G42940 31.694 183 108 4 73 253 42 209 1.05e-13 71.2
MS.gene22562.t1 AT3G04570 30.818 159 88 4 98 234 69 227 1.52e-11 65.5
MS.gene22562.t1 AT2G35270 35.385 130 80 2 123 250 104 231 1.84e-11 65.1
MS.gene22562.t1 AT4G17800 34.615 130 81 2 123 250 108 235 4.70e-11 63.9
MS.gene22562.t1 AT1G76500 29.508 122 75 2 124 234 99 220 8.84e-11 63.2

Find 109 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCGTCTGGTCCATACTTTC+TGG 0.172462 5.2:-4271475 None:intergenic
TTCCAGGTACTTACATTTAC+AGG 0.203699 5.2:-4272590 None:intergenic
AAGATAGGCTGTTAGGTATT+TGG 0.208183 5.2:-4274316 None:intergenic
TGGATTCTCCAACTCGAATT+TGG 0.209769 5.2:-4271553 None:intergenic
CGCATTATCACAGTTAATTC+TGG 0.217530 5.2:+4272022 MS.gene22562:CDS
TATGAAATTCGAACTTTATC+TGG 0.233802 5.2:+4273811 MS.gene22562:CDS
TATGAAATTATATCTTTATC+TGG 0.243485 5.2:+4272458 MS.gene22562:CDS
GACCAGGTACTTACATTTAC+AGG 0.268526 5.2:-4273958 None:intergenic
ATTCCAGTTCTTGGTGCTGC+AGG 0.307459 5.2:-4271354 None:intergenic
CGCGGAAGGCGGAGTATATC+TGG 0.344559 5.2:+4273856 MS.gene22562:CDS
AGGGGTCGCCCCAGAAAGTA+TGG 0.356183 5.2:+4271465 MS.gene22562:CDS
CCGTCTGGTCCATACTTTCT+GGG 0.367431 5.2:-4271474 None:intergenic
TGTTGAGTTTGATCTTCAAT+AGG 0.372062 5.2:-4271950 None:intergenic
GATAGAATGCATATGGCTTC+TGG 0.380389 5.2:-4273562 None:intergenic
TGGCATGAGTGTTTCTTTAG+TGG 0.383981 5.2:+4273879 MS.gene22562:CDS
AAAGCTGGGATTCCAGTTCT+TGG 0.386982 5.2:-4271363 None:intergenic
GATGTAAAAGTAGTGCCTTC+TGG 0.395117 5.2:-4271998 None:intergenic
GTTGAGTTTGATCTTCAATA+GGG 0.398662 5.2:-4271949 None:intergenic
TTCTGCTCATGTTGTTCACT+TGG 0.400794 5.2:-4274232 None:intergenic
GTTTCTTTAGCGGGTCTAGA+TGG 0.404137 5.2:+4272521 MS.gene22562:CDS
ATCGTTGGAGGTTGCGTAGC+TGG 0.408342 5.2:+4272548 MS.gene22562:CDS
ACCAGACGGGTCTTTAATCT+TGG 0.410529 5.2:+4271488 MS.gene22562:CDS
GATAGAATGCATATAGCTTC+TGG 0.411982 5.2:-4272224 None:intergenic
CCACCACTGCGACTTTCGTT+TGG 0.412733 5.2:-4272491 None:intergenic
AACCCTGCAGCACCAAGAAC+TGG 0.424961 5.2:+4271351 MS.gene22562:CDS
GCAGTAGACATGGGAGCAGC+TGG 0.426144 5.2:-4274289 None:intergenic
GTGGGTCCATCGGCAAAAGC+TGG 0.432662 5.2:-4271378 None:intergenic
CCTTATGCTCTAGTTCTTGA+GGG 0.436524 5.2:-4271973 None:intergenic
CACAGCTGATTCGACATCTC+AGG 0.441577 5.2:+4271398 MS.gene22562:CDS
TGGCGCCAGCAAAGAGGAAA+AGG 0.445228 5.2:+4271445 MS.gene22562:CDS
GGTATGAGTGTTTCTTTAGC+GGG 0.447972 5.2:+4272512 MS.gene22562:CDS
GTTGTTGGCGGTCGTGTAGC+TGG 0.449009 5.2:+4273916 MS.gene22562:CDS
TAACTGAGCTTGCAAGCAAT+TGG 0.450726 5.2:-4273244 None:intergenic
GGCGCCACCATTTGCAGCGG+TGG 0.453790 5.2:-4271429 None:intergenic
AGGCATTCCACCGCTGCAAA+TGG 0.457742 5.2:+4271422 MS.gene22562:CDS
ACCTTATGCTCTAGTTCTTG+AGG 0.457940 5.2:-4271974 None:intergenic
ATTCACAGATAGAATGCATA+TGG 0.464355 5.2:-4273569 None:intergenic
GCTGCTCCCATGTCTACTGC+TGG 0.473409 5.2:+4274292 MS.gene22562:CDS
CCCTCAAGAACTAGAGCATA+AGG 0.474150 5.2:+4271973 MS.gene22562:CDS
GGCGCCAGCAAAGAGGAAAA+GGG 0.481838 5.2:+4271446 MS.gene22562:CDS
ATATGCATTCTATCTGCAAA+TGG 0.495626 5.2:+4272233 MS.gene22562:CDS
ATGGAGGGTGCTAGCAATAG+TGG 0.502183 5.2:+4271306 MS.gene22562:CDS
GGTGCTGCAGGGTTATCTGT+TGG 0.503393 5.2:-4271342 None:intergenic
TTCCAGTTCTTGGTGCTGCA+GGG 0.504119 5.2:-4271353 None:intergenic
ATGGAGCTAGATGTTGGCTT+GGG 0.508152 5.2:-4271519 None:intergenic
TCGAACATTATTCACGTATG+AGG 0.511504 5.2:+4273633 MS.gene22562:CDS
GATGCTCCCGTGTCTACTGC+TGG 0.518304 5.2:+4272949 MS.gene22562:CDS
ATCTGGTTTGTTCATGCCAG+AGG 0.521232 5.2:+4273828 MS.gene22562:CDS
AGACGATTGAGGCTGACTAA+TGG 0.522248 5.2:-4272273 None:intergenic
TTGCCGATGGAGCTAGATGT+TGG 0.522643 5.2:-4271525 None:intergenic
AATAATGTTCGAGACGATTG+AGG 0.525993 5.2:-4273622 None:intergenic
CTGAGATGTCGAATCAGCTG+TGG 0.534004 5.2:-4271397 None:intergenic
GTTTCTTTAGTGGATCTACA+TGG 0.535434 5.2:+4273889 MS.gene22562:CDS
ATATGCATTCTATCTGTGAA+TGG 0.536186 5.2:+4273571 MS.gene22562:CDS
GCATTCACAATGAAAATATG+AGG 0.537005 5.2:-4273281 None:intergenic
AGAATTAACTGTGATAATGC+GGG 0.538033 5.2:-4272020 None:intergenic
TGCCAGAGGAAAAGCGCGGA+AGG 0.539759 5.2:+4273842 MS.gene22562:CDS
TGGTATGAGTGTTTCTTTAG+CGG 0.540533 5.2:+4272511 MS.gene22562:CDS
ACAGGACTTGCAGCCACCAA+TGG 0.541015 5.2:-4273940 None:intergenic
CGAATCAGCTGTGGGTCCAT+CGG 0.541484 5.2:-4271388 None:intergenic
CATTCCACCGCTGCAAATGG+TGG 0.541527 5.2:+4271425 MS.gene22562:CDS
GATGGAGCTAGATGTTGGCT+TGG 0.542943 5.2:-4271520 None:intergenic
CGGTCGTGTAGCTGGTCCAT+TGG 0.545745 5.2:+4273924 MS.gene22562:CDS
GAAATGCTTCACCTCATATG+TGG 0.547537 5.2:-4272306 None:intergenic
ACAGGACTTGCAGCTACCAA+TGG 0.547952 5.2:-4272572 None:intergenic
GAATTTGGCAGCTTTGCCGA+TGG 0.548498 5.2:-4271538 None:intergenic
TCGTGTAGCTGGTCCATTGG+TGG 0.554685 5.2:+4273927 MS.gene22562:CDS
GGTCTAGATGGACATATCGT+TGG 0.556357 5.2:+4272533 MS.gene22562:CDS
TGGGTCCATCGGCAAAAGCT+GGG 0.559063 5.2:-4271377 None:intergenic
GCCAAGATTAAAGACCCGTC+TGG 0.560561 5.2:-4271489 None:intergenic
ATTTGGACCAGCAGTAGACA+TGG 0.563774 5.2:-4274299 None:intergenic
CAAAGCTGCCAAATTCGAGT+TGG 0.564111 5.2:+4271545 MS.gene22562:CDS
CAGAATTAACTGTGATAATG+CGG 0.564469 5.2:-4272021 None:intergenic
GATCTACATGGACATGTTGT+TGG 0.565136 5.2:+4273901 MS.gene22562:CDS
CTAGATGGACATATCGTTGG+AGG 0.567496 5.2:+4272536 MS.gene22562:CDS
GACACGGGAGCATCTGCCGT+TGG 0.568071 5.2:-4272940 None:intergenic
TTTCATTGTGAATGCTGGAG+AGG 0.570869 5.2:+4273289 MS.gene22562:CDS
AAGCCAACATCTAGCTCCAT+CGG 0.574453 5.2:+4271522 MS.gene22562:CDS
CCCAGAAAGTATGGACCAGA+CGG 0.575081 5.2:+4271474 MS.gene22562:CDS
AGGTTGCGTAGCTGGTCCAT+TGG 0.577220 5.2:+4272556 MS.gene22562:CDS
GCTGGCGCCACCATTTGCAG+CGG 0.582574 5.2:-4271432 None:intergenic
TATCAAAAGCAGTCATTAGT+CGG 0.588120 5.2:+4273599 MS.gene22562:CDS
GAGCTAGATGTTGGCTTGGG+AGG 0.595395 5.2:-4271516 None:intergenic
AAATTCGAGTTGGAGAATCC+AGG 0.596763 5.2:+4271555 MS.gene22562:CDS
CTACATGGACATGTTGTTGG+CGG 0.596833 5.2:+4273904 MS.gene22562:CDS
GACTTCAACGTCAATGCCAA+CGG 0.603268 5.2:+4272924 MS.gene22562:CDS
GATATGTATCAGGTAGTGGT+AGG 0.603294 5.2:+4274199 MS.gene22562:intron
CTAGAGCATAAGGTGCCAGA+AGG 0.609617 5.2:+4271983 MS.gene22562:CDS
TATTGTACCAGCAGTAGACA+CGG 0.609633 5.2:-4272956 None:intergenic
AGTAAATTTAGATCCTCATG+AGG 0.610048 5.2:+4271925 MS.gene22562:CDS
CGTCTGGTCCATACTTTCTG+GGG 0.621397 5.2:-4271473 None:intergenic
CCAAACGAAAGTCGCAGTGG+TGG 0.623451 5.2:+4272491 MS.gene22562:CDS
ACAGCTGATTCGACATCTCA+GGG 0.628768 5.2:+4271399 MS.gene22562:CDS
CAGAGGAAAAGCGCGGAAGG+CGG 0.629538 5.2:+4273845 MS.gene22562:CDS
GCGCCAGCAAAGAGGAAAAG+GGG 0.629697 5.2:+4271447 MS.gene22562:CDS
TGAGATGTCGAATCAGCTGT+GGG 0.630120 5.2:-4271396 None:intergenic
TATCACAGTTAATTCTGGAG+AGG 0.631106 5.2:+4272027 MS.gene22562:CDS
AAATGGTGGCGCCAGCAAAG+AGG 0.640999 5.2:+4271439 MS.gene22562:CDS
CGATTGAGGCTGACTAATGG+TGG 0.644722 5.2:-4272270 None:intergenic
CCAGAAAGTATGGACCAGAC+GGG 0.646931 5.2:+4271475 MS.gene22562:CDS
GTAAATTTAGATCCTCATGA+GGG 0.647623 5.2:+4271926 MS.gene22562:CDS
TCTTCAATAGGGCCCTCATG+AGG 0.663045 5.2:-4271938 None:intergenic
ACTCTAGTGAAGTTCATGGA+GGG 0.668635 5.2:+4271291 None:intergenic
TTTGGACCAGCAGTAGACAT+GGG 0.690481 5.2:-4274298 None:intergenic
ATTGTACCAGCAGTAGACAC+GGG 0.698737 5.2:-4272955 None:intergenic
GTGCCAAACGAAAGTCGCAG+TGG 0.704907 5.2:+4272488 MS.gene22562:CDS
GGAAGGCGGAGTATATCTGG+TGG 0.708998 5.2:+4273859 MS.gene22562:CDS
TTCATGCCAGAGGAAAAGCG+CGG 0.748400 5.2:+4273838 MS.gene22562:CDS
GCTGATTCGACATCTCAGGG+AGG 0.790272 5.2:+4271402 MS.gene22562:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATATATATCAAGTTAT+TGG + chr5.2:4273107-4273126 MS.gene22562:intron 10.0%
!! TATCTAATTATGAATATTAT+GGG - chr5.2:4273359-4273378 None:intergenic 10.0%
!! TTATCTAATTATGAATATTA+TGG - chr5.2:4273360-4273379 None:intergenic 10.0%
!! TATGAAATTATATCTTTATC+TGG + chr5.2:4272458-4272477 MS.gene22562:CDS 15.0%
!! TTAATATATATCAAGTGTAT+TGG + chr5.2:4271693-4271712 MS.gene22562:intron 15.0%
!!! TATATCTTGATATTTTTAAC+TGG + chr5.2:4273387-4273406 MS.gene22562:intron 15.0%
!! AATAATGCAAATTGAATTTC+AGG + chr5.2:4273783-4273802 MS.gene22562:intron 20.0%
!! AATAATGTCAATTGAATTTC+AGG + chr5.2:4272430-4272449 MS.gene22562:intron 20.0%
!! AATATATATCAAGTTATTGG+TGG + chr5.2:4273110-4273129 MS.gene22562:intron 20.0%
!! ATAATGCAAATTGAATTTCA+GGG + chr5.2:4273784-4273803 MS.gene22562:intron 20.0%
!! ATAATGTCAATTGAATTTCA+GGG + chr5.2:4272431-4272450 MS.gene22562:intron 20.0%
!! ATATCTTCGATATTCATAAT+TGG + chr5.2:4272052-4272071 MS.gene22562:intron 20.0%
!! TAAGATATAAGTGTAAATGT+TGG + chr5.2:4273147-4273166 MS.gene22562:intron 20.0%
!! TAAGATGTAATTGTAAATGT+TGG + chr5.2:4271734-4271753 MS.gene22562:intron 20.0%
!! TAATCTGATAGTAGTATTAT+TGG + chr5.2:4272331-4272350 MS.gene22562:intron 20.0%
!! TAATGACTGATTAGAATAAA+TGG + chr5.2:4272135-4272154 MS.gene22562:intron 20.0%
!! TTATATCTTAAATTGATGCA+AGG - chr5.2:4273137-4273156 None:intergenic 20.0%
!!! TATTGGTATCAGTTTTAATA+TGG + chr5.2:4272348-4272367 MS.gene22562:intron 20.0%
! ATATATATCAAGTGTATTGG+TGG + chr5.2:4271696-4271715 MS.gene22562:intron 25.0%
! GCTTATGTAGTTATTTATCT+TGG + chr5.2:4274027-4274046 MS.gene22562:intron 25.0%
! TATGAAATTCGAACTTTATC+TGG + chr5.2:4273811-4273830 MS.gene22562:CDS 25.0%
! TCAAAAATTATCCACATATG+AGG + chr5.2:4272295-4272314 MS.gene22562:CDS 25.0%
! TGATGACTAATCAGAATAAA+TGG + chr5.2:4273473-4273492 MS.gene22562:intron 25.0%
! TGTTCATAATTTCCTGATTT+CGG + chr5.2:4271805-4271824 MS.gene22562:intron 25.0%
! TTAAAGAAACACATCAAAAC+AGG - chr5.2:4272411-4272430 None:intergenic 25.0%
!! ATGCAATTCTGATAATTTTG+TGG + chr5.2:4274121-4274140 MS.gene22562:intron 25.0%
!!! GAATAGTGTATTTTGTTGTT+TGG + chr5.2:4273066-4273085 MS.gene22562:intron 25.0%
!!! TCAGTTTTAATATGGAGTTT+AGG + chr5.2:4272356-4272375 MS.gene22562:intron 25.0%
AATGTACTAATGTGGAGTTT+AGG + chr5.2:4273711-4273730 MS.gene22562:intron 30.0%
AGTAAATTTAGATCCTCATG+AGG + chr5.2:4271925-4271944 MS.gene22562:CDS 30.0%
ATACTAAAACACCAATTAGC+AGG + chr5.2:4272643-4272662 MS.gene22562:intron 30.0%
ATATGCATTCTATCTGCAAA+TGG + chr5.2:4272233-4272252 MS.gene22562:CDS 30.0%
ATATGCATTCTATCTGTGAA+TGG + chr5.2:4273571-4273590 MS.gene22562:CDS 30.0%
ATTCACAGATAGAATGCATA+TGG - chr5.2:4273572-4273591 None:intergenic 30.0%
ATTCTCATTACTCTTTCCAA+TGG + chr5.2:4272818-4272837 MS.gene22562:intron 30.0%
CAACTGAATTTGCAAGAAAT+TGG - chr5.2:4271898-4271917 None:intergenic 30.0%
CAATTTCTTGCAAATTCAGT+TGG + chr5.2:4271896-4271915 MS.gene22562:intron 30.0%
CCACATTAGTACATTGAATT+AGG - chr5.2:4273706-4273725 None:intergenic 30.0%
CCTAATTCAATGTACTAATG+TGG + chr5.2:4273703-4273722 MS.gene22562:intron 30.0%
GCATTCACAATGAAAATATG+AGG - chr5.2:4273284-4273303 None:intergenic 30.0%
GCTAAAAACACAGAATAATG+AGG + chr5.2:4274255-4274274 MS.gene22562:CDS 30.0%
GTAAATTTAGATCCTCATGA+GGG + chr5.2:4271926-4271945 MS.gene22562:CDS 30.0%
GTTGAGTTTGATCTTCAATA+GGG - chr5.2:4271952-4271971 None:intergenic 30.0%
TAAATGTAAGTACCTGGAAA+GGG + chr5.2:4272594-4272613 MS.gene22562:intron 30.0%
TATCAAAAGCAGTCATTAGT+CGG + chr5.2:4273599-4273618 MS.gene22562:CDS 30.0%
TGAATAACAAACTACTCCTA+AGG - chr5.2:4273738-4273757 None:intergenic 30.0%
TGTTGAGTTTGATCTTCAAT+AGG - chr5.2:4271953-4271972 None:intergenic 30.0%
! AGAATTAACTGTGATAATGC+GGG - chr5.2:4272023-4272042 None:intergenic 30.0%
! CAGAATTAACTGTGATAATG+CGG - chr5.2:4272024-4272043 None:intergenic 30.0%
! CATATTTTCATTGTGAATGC+TGG + chr5.2:4273284-4273303 MS.gene22562:CDS 30.0%
! GTTCTTTCTTTCTTTTTGTC+TGG + chr5.2:4271872-4271891 MS.gene22562:intron 30.0%
!!! AAGTGCTTTCTTTTTTTCTC+AGG + chr5.2:4273221-4273240 MS.gene22562:intron 30.0%
!!! ATAATGTCATTTTGTCAGCA+AGG + chr5.2:4273538-4273557 MS.gene22562:CDS 30.0%
!!! GATAATTTTTGAGACGATTG+AGG - chr5.2:4272287-4272306 None:intergenic 30.0%
!!! GGAGAGGTAATATCATTTTT+TGG + chr5.2:4273305-4273324 MS.gene22562:intron 30.0%
AAGAGAATTTCTTAGCTACC+TGG - chr5.2:4271576-4271595 None:intergenic 35.0%
AATAATGTTCGAGACGATTG+AGG - chr5.2:4273625-4273644 None:intergenic 35.0%
AGATATTCAATGCCGAAATC+AGG - chr5.2:4271820-4271839 None:intergenic 35.0%
AGTCATCATTCCAGTGATAA+CGG - chr5.2:4273461-4273480 None:intergenic 35.0%
ATAGTAGCAATTTCGTTAGC+AGG - chr5.2:4272739-4272758 None:intergenic 35.0%
ATTCTCATCACTGTTTCCAA+TGG + chr5.2:4274173-4274192 MS.gene22562:intron 35.0%
CCTGATACATATCAATCCAT+TGG - chr5.2:4272837-4272856 None:intergenic 35.0%
CGCATTATCACAGTTAATTC+TGG + chr5.2:4272022-4272041 MS.gene22562:CDS 35.0%
GATAGAATGCATATAGCTTC+TGG - chr5.2:4272227-4272246 None:intergenic 35.0%
GATTGATATGTATCAGGTAG+TGG + chr5.2:4272840-4272859 MS.gene22562:intron 35.0%
GATTTAAGCATCCTGCTAAT+TGG - chr5.2:4272657-4272676 None:intergenic 35.0%
GTAAATGTAAGTACCTGGAA+AGG + chr5.2:4272593-4272612 MS.gene22562:intron 35.0%
GTAGTAAAACGCAAACTTTG+TGG + chr5.2:4272766-4272785 MS.gene22562:intron 35.0%
GTATTTGTTTCCGTTATCAC+TGG + chr5.2:4273448-4273467 MS.gene22562:intron 35.0%
TACCTGGAAAGGGAATAATA+GGG + chr5.2:4272604-4272623 MS.gene22562:intron 35.0%
TCGAACATTATTCACGTATG+AGG + chr5.2:4273633-4273652 MS.gene22562:CDS 35.0%
TCTGTTTCCTGCTAAAGAAA+TGG + chr5.2:4274080-4274099 MS.gene22562:intron 35.0%
TGGTATGAGTGTTTCTTTAG+CGG + chr5.2:4272511-4272530 MS.gene22562:CDS 35.0%
TTCCAGGTACTTACATTTAC+AGG - chr5.2:4272593-4272612 None:intergenic 35.0%
! AATGGCTACTACTTGTCTTT+TGG + chr5.2:4272153-4272172 MS.gene22562:intron 35.0%
! ATAGTTGCCATTTCTTTAGC+AGG - chr5.2:4274090-4274109 None:intergenic 35.0%
! GTTTCTTTAGTGGATCTACA+TGG + chr5.2:4273889-4273908 MS.gene22562:CDS 35.0%
! TATCACAGTTAATTCTGGAG+AGG + chr5.2:4272027-4272046 MS.gene22562:CDS 35.0%
! TTCGGCATTGAATATCTTTG+TGG + chr5.2:4271823-4271842 MS.gene22562:intron 35.0%
!! ACTTGTCTTTTGATTGTCTC+AGG + chr5.2:4273501-4273520 MS.gene22562:intron 35.0%
!! CCAATGGATTGATATGTATC+AGG + chr5.2:4272834-4272853 MS.gene22562:intron 35.0%
!! GAATAGTGCACTTTGTTGTT+CGG + chr5.2:4271651-4271670 MS.gene22562:intron 35.0%
!!! GTAATGTCATTTTGTCAGCA+AGG + chr5.2:4272200-4272219 MS.gene22562:CDS 35.0%
AAAAGACATATTCCCTAGCC+AGG - chr5.2:4273983-4274002 None:intergenic 40.0%
AAGAAAGAGAATCATCTCCG+TGG - chr5.2:4273007-4273026 None:intergenic 40.0%
GAAATGCTTCACCTCATATG+TGG - chr5.2:4272309-4272328 None:intergenic 40.0%
GACCAGGTACTTACATTTAC+AGG - chr5.2:4273961-4273980 None:intergenic 40.0%
GATAGAATGCATATGGCTTC+TGG - chr5.2:4273565-4273584 None:intergenic 40.0%
GATCTACATGGACATGTTGT+TGG + chr5.2:4273901-4273920 MS.gene22562:CDS 40.0%
GATGTAAAAGTAGTGCCTTC+TGG - chr5.2:4272001-4272020 None:intergenic 40.0%
GCTAAAAACGCAGGAAAATG+AGG + chr5.2:4272900-4272919 MS.gene22562:CDS 40.0%
GGAAGATGAAGATGAGAGTT+AGG - chr5.2:4272982-4273001 None:intergenic 40.0%
GGTATGAGTGTTTCTTTAGC+GGG + chr5.2:4272512-4272531 MS.gene22562:CDS 40.0%
GTACCTGGAAAGGGAATAAT+AGG + chr5.2:4272603-4272622 MS.gene22562:intron 40.0%
GTCCTGTAAATGTAAGTACC+TGG + chr5.2:4272588-4272607 MS.gene22562:intron 40.0%
TAAATGTAAGTACCTGGTCC+TGG + chr5.2:4273962-4273981 MS.gene22562:intron 40.0%
TAACTGAGCTTGCAAGCAAT+TGG - chr5.2:4273247-4273266 None:intergenic 40.0%
TATTGTACCAGCAGTAGACA+CGG - chr5.2:4272959-4272978 None:intergenic 40.0%
TCATCTTCATCTTCCTTCCA+CGG + chr5.2:4272987-4273006 MS.gene22562:intron 40.0%
TGGATTCTCCAACTCGAATT+TGG - chr5.2:4271556-4271575 None:intergenic 40.0%
TGGCATGAGTGTTTCTTTAG+TGG + chr5.2:4273879-4273898 MS.gene22562:CDS 40.0%
TTCGCAAACTAAAGTCTAGC+AGG + chr5.2:4274002-4274021 MS.gene22562:intron 40.0%
TTCTGCTCATGTTGTTCACT+TGG - chr5.2:4274235-4274254 None:intergenic 40.0%
TTGTGATCATGCGAGTTTCA+TGG + chr5.2:4273026-4273045 MS.gene22562:intron 40.0%
TTTCATTGTGAATGCTGGAG+AGG + chr5.2:4273289-4273308 MS.gene22562:CDS 40.0%
! AATGTGGAGTTTAGGACCTT+AGG + chr5.2:4273719-4273738 MS.gene22562:intron 40.0%
! GATATGTATCAGGTAGTGGT+AGG + chr5.2:4272844-4272863 MS.gene22562:intron 40.0%
! GTTTGCGTTTTACTACACAC+TGG - chr5.2:4272762-4272781 None:intergenic 40.0%
!! AAATTCGAGTTGGAGAATCC+AGG + chr5.2:4271555-4271574 MS.gene22562:CDS 40.0%
!! ACCTTATGCTCTAGTTCTTG+AGG - chr5.2:4271977-4271996 None:intergenic 40.0%
!! CCTTATGCTCTAGTTCTTGA+GGG - chr5.2:4271976-4271995 None:intergenic 40.0%
AAAGCTGGGATTCCAGTTCT+TGG - chr5.2:4271366-4271385 None:intergenic 45.0%
AAGAGAATCATCTCCGTGGA+AGG - chr5.2:4273003-4273022 None:intergenic 45.0%
ACCAGACGGGTCTTTAATCT+TGG + chr5.2:4271488-4271507 MS.gene22562:CDS 45.0%
AGACGATTGAGGCTGACTAA+TGG - chr5.2:4272276-4272295 None:intergenic 45.0%
AGCCCTATTATTCCCTTTCC+AGG - chr5.2:4272609-4272628 None:intergenic 45.0%
ATTGTACCAGCAGTAGACAC+GGG - chr5.2:4272958-4272977 None:intergenic 45.0%
ATTTGGACCAGCAGTAGACA+TGG - chr5.2:4274302-4274321 None:intergenic 45.0%
CAAAGCTGCCAAATTCGAGT+TGG + chr5.2:4271545-4271564 MS.gene22562:CDS 45.0%
CCCTCAAGAACTAGAGCATA+AGG + chr5.2:4271973-4271992 MS.gene22562:CDS 45.0%
CTACATGGACATGTTGTTGG+CGG + chr5.2:4273904-4273923 MS.gene22562:CDS 45.0%
CTAGATGGACATATCGTTGG+AGG + chr5.2:4272536-4272555 MS.gene22562:CDS 45.0%
GACTTCAACGTCAATGCCAA+CGG + chr5.2:4272924-4272943 MS.gene22562:CDS 45.0%
GGTCTAGATGGACATATCGT+TGG + chr5.2:4272533-4272552 MS.gene22562:CDS 45.0%
TCATGCGAGTTTCATGGTTG+TGG + chr5.2:4273032-4273051 MS.gene22562:intron 45.0%
TGAGATGTCGAATCAGCTGT+GGG - chr5.2:4271399-4271418 None:intergenic 45.0%
TGAGCAGAAGCTAAAAACGC+AGG + chr5.2:4272891-4272910 MS.gene22562:CDS 45.0%
TTTGGACCAGCAGTAGACAT+GGG - chr5.2:4274301-4274320 None:intergenic 45.0%
! AAGCCAACATCTAGCTCCAT+CGG + chr5.2:4271522-4271541 MS.gene22562:CDS 45.0%
! GTTTCTTTAGCGGGTCTAGA+TGG + chr5.2:4272521-4272540 MS.gene22562:CDS 45.0%
!! ACAGCTGATTCGACATCTCA+GGG + chr5.2:4271399-4271418 MS.gene22562:CDS 45.0%
!! ATCTGGTTTGTTCATGCCAG+AGG + chr5.2:4273828-4273847 MS.gene22562:CDS 45.0%
!! ATGGAGCTAGATGTTGGCTT+GGG - chr5.2:4271522-4271541 None:intergenic 45.0%
!! GGTAGTGGTAAGCAGTTTTC+TGG + chr5.2:4272855-4272874 MS.gene22562:intron 45.0%
ACAGGACTTGCAGCTACCAA+TGG - chr5.2:4272575-4272594 None:intergenic 50.0%
CCGTCTGGTCCATACTTTCT+GGG - chr5.2:4271477-4271496 None:intergenic 50.0%
CGATTGAGGCTGACTAATGG+TGG - chr5.2:4272273-4272292 None:intergenic 50.0%
CGTCTGGTCCATACTTTCTG+GGG - chr5.2:4271476-4271495 None:intergenic 50.0%
CTGAGATGTCGAATCAGCTG+TGG - chr5.2:4271400-4271419 None:intergenic 50.0%
GAATTTGGCAGCTTTGCCGA+TGG - chr5.2:4271541-4271560 None:intergenic 50.0%
GCCAAGATTAAAGACCCGTC+TGG - chr5.2:4271492-4271511 None:intergenic 50.0%
TAAGTACCTGGTCCTGGCTA+GGG + chr5.2:4273968-4273987 MS.gene22562:intron 50.0%
TCTTCAATAGGGCCCTCATG+AGG - chr5.2:4271941-4271960 None:intergenic 50.0%
TGCGAGTTTCATGGTTGTGG+AGG + chr5.2:4273035-4273054 MS.gene22562:intron 50.0%
TTCATGCCAGAGGAAAAGCG+CGG + chr5.2:4273838-4273857 MS.gene22562:CDS 50.0%
TTGCCGATGGAGCTAGATGT+TGG - chr5.2:4271528-4271547 None:intergenic 50.0%
! CCAGAAAGTATGGACCAGAC+GGG + chr5.2:4271475-4271494 MS.gene22562:CDS 50.0%
! CCCAGAAAGTATGGACCAGA+CGG + chr5.2:4271474-4271493 MS.gene22562:CDS 50.0%
! CTAGAGCATAAGGTGCCAGA+AGG + chr5.2:4271983-4272002 MS.gene22562:CDS 50.0%
! GATGGAGCTAGATGTTGGCT+TGG - chr5.2:4271523-4271542 None:intergenic 50.0%
!! ATGGAGGGTGCTAGCAATAG+TGG + chr5.2:4271306-4271325 MS.gene22562:CDS 50.0%
!! ATTCCAGTTCTTGGTGCTGC+AGG - chr5.2:4271357-4271376 None:intergenic 50.0%
!! CACAGCTGATTCGACATCTC+AGG + chr5.2:4271398-4271417 MS.gene22562:CDS 50.0%
!! TTCCAGTTCTTGGTGCTGCA+GGG - chr5.2:4271356-4271375 None:intergenic 50.0%
AACCCTGCAGCACCAAGAAC+TGG + chr5.2:4271351-4271370 MS.gene22562:CDS 55.0%
ACAGGACTTGCAGCCACCAA+TGG - chr5.2:4273943-4273962 None:intergenic 55.0%
AGGCATTCCACCGCTGCAAA+TGG + chr5.2:4271422-4271441 MS.gene22562:CDS 55.0%
AGGTTGCGTAGCTGGTCCAT+TGG + chr5.2:4272556-4272575 MS.gene22562:CDS 55.0%
ATCGTTGGAGGTTGCGTAGC+TGG + chr5.2:4272548-4272567 MS.gene22562:CDS 55.0%
CATATTCCCTAGCCAGGACC+AGG - chr5.2:4273977-4273996 None:intergenic 55.0%
CATTCCACCGCTGCAAATGG+TGG + chr5.2:4271425-4271444 MS.gene22562:CDS 55.0%
CCAAACGAAAGTCGCAGTGG+TGG + chr5.2:4272491-4272510 MS.gene22562:CDS 55.0%
CCACCACTGCGACTTTCGTT+TGG - chr5.2:4272494-4272513 None:intergenic 55.0%
CCCGTCTGGTCCATACTTTC+TGG - chr5.2:4271478-4271497 None:intergenic 55.0%
CGAATCAGCTGTGGGTCCAT+CGG - chr5.2:4271391-4271410 None:intergenic 55.0%
GCGCCAGCAAAGAGGAAAAG+GGG + chr5.2:4271447-4271466 MS.gene22562:CDS 55.0%
GGAAGGCGGAGTATATCTGG+TGG + chr5.2:4273859-4273878 MS.gene22562:CDS 55.0%
GGCGCCAGCAAAGAGGAAAA+GGG + chr5.2:4271446-4271465 MS.gene22562:CDS 55.0%
GTAAGTACCTGGTCCTGGCT+AGG + chr5.2:4273967-4273986 MS.gene22562:intron 55.0%
GTGCCAAACGAAAGTCGCAG+TGG + chr5.2:4272488-4272507 MS.gene22562:CDS 55.0%
TCGTGTAGCTGGTCCATTGG+TGG + chr5.2:4273927-4273946 MS.gene22562:CDS 55.0%
TGGCGCCAGCAAAGAGGAAA+AGG + chr5.2:4271445-4271464 MS.gene22562:CDS 55.0%
TGGGTCCATCGGCAAAAGCT+GGG - chr5.2:4271380-4271399 None:intergenic 55.0%
! AAATGGTGGCGCCAGCAAAG+AGG + chr5.2:4271439-4271458 MS.gene22562:CDS 55.0%
! CGACCCCTTTTCCTCTTTGC+TGG - chr5.2:4271453-4271472 None:intergenic 55.0%
! GGAATCCCAGCTTTTGCCGA+TGG + chr5.2:4271372-4271391 MS.gene22562:CDS 55.0%
! GGTGCTGCAGGGTTATCTGT+TGG - chr5.2:4271345-4271364 None:intergenic 55.0%
!! GAGCTAGATGTTGGCTTGGG+AGG - chr5.2:4271519-4271538 None:intergenic 55.0%
!! GCTGATTCGACATCTCAGGG+AGG + chr5.2:4271402-4271421 MS.gene22562:CDS 55.0%
AGGGGTCGCCCCAGAAAGTA+TGG + chr5.2:4271465-4271484 MS.gene22562:CDS 60.0%
CGCGGAAGGCGGAGTATATC+TGG + chr5.2:4273856-4273875 MS.gene22562:CDS 60.0%
CGGTCGTGTAGCTGGTCCAT+TGG + chr5.2:4273924-4273943 MS.gene22562:CDS 60.0%
GATGCTCCCGTGTCTACTGC+TGG + chr5.2:4272949-4272968 MS.gene22562:CDS 60.0%
GCAGTAGACATGGGAGCAGC+TGG - chr5.2:4274292-4274311 None:intergenic 60.0%
GCTGCTCCCATGTCTACTGC+TGG + chr5.2:4274292-4274311 MS.gene22562:CDS 60.0%
GTGGGTCCATCGGCAAAAGC+TGG - chr5.2:4271381-4271400 None:intergenic 60.0%
GTTGTTGGCGGTCGTGTAGC+TGG + chr5.2:4273916-4273935 MS.gene22562:CDS 60.0%
! CAGAGGAAAAGCGCGGAAGG+CGG + chr5.2:4273845-4273864 MS.gene22562:CDS 60.0%
! TGCCAGAGGAAAAGCGCGGA+AGG + chr5.2:4273842-4273861 MS.gene22562:CDS 60.0%
GCTGGCGCCACCATTTGCAG+CGG - chr5.2:4271435-4271454 None:intergenic 65.0%
! CGCCTTCCGCGCTTTTCCTC+TGG - chr5.2:4273847-4273866 None:intergenic 65.0%
!! GACACGGGAGCATCTGCCGT+TGG - chr5.2:4272943-4272962 None:intergenic 65.0%
GGCGCCACCATTTGCAGCGG+TGG - chr5.2:4271432-4271451 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 4271306 4274327 4271306 ID=MS.gene22562
chr5.2 mRNA 4271306 4274327 4271306 ID=MS.gene22562.t1;Parent=MS.gene22562
chr5.2 exon 4271306 4271576 4271306 ID=MS.gene22562.t1.exon1;Parent=MS.gene22562.t1
chr5.2 CDS 4271306 4271576 4271306 ID=cds.MS.gene22562.t1;Parent=MS.gene22562.t1
chr5.2 exon 4271915 4272048 4271915 ID=MS.gene22562.t1.exon2;Parent=MS.gene22562.t1
chr5.2 CDS 4271915 4272048 4271915 ID=cds.MS.gene22562.t1;Parent=MS.gene22562.t1
chr5.2 exon 4272185 4272316 4272185 ID=MS.gene22562.t1.exon3;Parent=MS.gene22562.t1
chr5.2 CDS 4272185 4272316 4272185 ID=cds.MS.gene22562.t1;Parent=MS.gene22562.t1
chr5.2 exon 4272452 4272598 4272452 ID=MS.gene22562.t1.exon4;Parent=MS.gene22562.t1
chr5.2 CDS 4272452 4272598 4272452 ID=cds.MS.gene22562.t1;Parent=MS.gene22562.t1
chr5.2 exon 4272856 4272970 4272856 ID=MS.gene22562.t1.exon5;Parent=MS.gene22562.t1
chr5.2 CDS 4272856 4272970 4272856 ID=cds.MS.gene22562.t1;Parent=MS.gene22562.t1
chr5.2 exon 4273243 4273310 4273243 ID=MS.gene22562.t1.exon6;Parent=MS.gene22562.t1
chr5.2 CDS 4273243 4273310 4273243 ID=cds.MS.gene22562.t1;Parent=MS.gene22562.t1
chr5.2 exon 4273523 4273654 4273523 ID=MS.gene22562.t1.exon7;Parent=MS.gene22562.t1
chr5.2 CDS 4273523 4273654 4273523 ID=cds.MS.gene22562.t1;Parent=MS.gene22562.t1
chr5.2 exon 4273805 4273966 4273805 ID=MS.gene22562.t1.exon8;Parent=MS.gene22562.t1
chr5.2 CDS 4273805 4273966 4273805 ID=cds.MS.gene22562.t1;Parent=MS.gene22562.t1
chr5.2 exon 4274211 4274327 4274211 ID=MS.gene22562.t1.exon9;Parent=MS.gene22562.t1
chr5.2 CDS 4274211 4274327 4274211 ID=cds.MS.gene22562.t1;Parent=MS.gene22562.t1
Gene Sequence

>MS.gene22562

ATGGAGGGTGCTAGCAATAGTGGTGCTTCAGATACTCCAACAGATAACCCTGCAGCACCAAGAACTGGAATCCCAGCTTTTGCCGATGGACCCACAGCTGATTCGACATCTCAGGGAGGCATTCCACCGCTGCAAATGGTGGCGCCAGCAAAGAGGAAAAGGGGTCGCCCCAGAAAGTATGGACCAGACGGGTCTTTAATCTTGGCGATACCTCCCAAGCCAACATCTAGCTCCATCGGCAAAGCTGCCAAATTCGAGTTGGAGAATCCAGGTAGCTAAGAAATTCTCTTTCTTTGTGATCTTGTGCATTTCTTGATTGAATGCTTGTGAATGAGTATCAAGAAAGAATAGTGCACTTTGTTGTTCGGAATGTTCAATGTTGTGTTATTAATATATATCAAGTGTATTGGTGGTACTTGCATCAAATATAAGATGTAATTGTAAATGTTGGTTCAGCTTTCATACATGTTGATTTTATTCTTTGAATAGAAACTGTGTCTGTTCATAATTTCCTGATTTCGGCATTGAATATCTTTGTGGTTAATTAGTATGATTATTTAGTGAAAGTTCTTTCTTTCTTTTTGTCTGGCCAATTTCTTGCAAATTCAGTTGGTGCTATAGTAAATTTAGATCCTCATGAGGGCCCTATTGAAGATCAAACTCAACACCCTCAAGAACTAGAGCATAAGGTGCCAGAAGGCACTACTTTTACATCCCGCATTATCACAGTTAATTCTGGAGAGGTAATATCTTCGATATTCATAATTGGTAGATGCACAAATTTAACTAAAATTTCTTGCAGTAATTATATTTATTTTCTATATAACTGTAATGACTGATTAGAATAAATGGCTACTACTTGTCTTTTGGTTGTCGCAGAATATTACTATGAAAGTAATGTCATTTTGTCAGCAAGGACCAGAAGCTATATGCATTCTATCTGCAAATGGAGTCATATCAAGCGCCACCATTAGTCAGCCTCAATCGTCTCAAAAATTATCCACATATGAGGTGAAGCATTTCTATAATCTGATAGTAGTATTATTGGTATCAGTTTTAATATGGAGTTTAGGTTATATAGCTTGTATTCAAATTTTGTTTGCCTGTTTTGATGTGTTTCTTTAAATAATGTCAATTGAATTTCAGGGTAAATATGAAATTATATCTTTATCTGGCTCATTCGTGCCAAACGAAAGTCGCAGTGGTGGTATGAGTGTTTCTTTAGCGGGTCTAGATGGACATATCGTTGGAGGTTGCGTAGCTGGTCCATTGGTAGCTGCAAGTCCTGTAAATGTAAGTACCTGGAAAGGGAATAATAGGGCTAATATGTTTTTCACATACTAAAACACCAATTAGCAGGATGCTTAAATCTTAATTATGTAGATATCTTTTGAAAAGTAAAGTTGATGATCTAGTAACATTATTAGTGTCCTGCTAACGAAATTGCTACTATCCAGTGTGTAGTAAAACGCAAACTTTGTGGTAACTAATTCTCAGTGACAAGTCTATGCAATTCTCATTACTCTTTCCAATGGATTGATATGTATCAGGTAGTGGTAAGCAGTTTTCTGGCAAATGAACAGTCTGAGCAGAAGCTAAAAACGCAGGAAAATGAGGCGACTTCAACGTCAATGCCAACGGCAGATGCTCCCGTGTCTACTGCTGGTACAATACCTAACTCTCATCTTCATCTTCCTTCCACGGAGATGATTCTCTTTCTTTGTGATCATGCGAGTTTCATGGTTGTGGAGGAGTATCAAGAATAGTGTATTTTGTTGTTTGGAATGTTTAATATTATATTATTAATATATATCAAGTTATTGGTGGTCCTTGCATCAATTTAAGATATAAGTGTAAATGTTGGTTCAGCTTTCTTACATGTTGATTGTATTCTTAAATGTTGATTATTTAGTGAAAGTGCTTTCTTTTTTTCTCAGGCCAATTGCTTGCAAGCTCAGTTAGTGCTACTTTTACACCTCATATTTTCATTGTGAATGCTGGAGAGGTAATATCATTTTTTGGATTTTTATGATGCAAAGAAATTCTATTTCCCATAATATTCATAATTAGATAAGTGAACATATATCTTGATATTTTTAACTGGTCAATGCACAACTTTAACTACAATTTCTTGCAATAATTGTATTTGTTTCCGTTATCACTGGAATGATGACTAATCAGAATAAATGGCTACTACTTGTCTTTTGATTGTCTCAGGATGTTTCGATGAAGATAATGTCATTTTGTCAGCAAGGACCAGAAGCCATATGCATTCTATCTGTGAATGGAGTCATATCAAAAGCAGTCATTAGTCGGCCTCAATCGTCTCGAACATTATTCACGTATGAGGTGAAGCATTTATGTAATAATCTGATAGTTGTAGTAGTATCAACTTTTCCTAATTCAATGTACTAATGTGGAGTTTAGGACCTTAGGAGTAGTTTGTTATTCATATGCTTTTTGTATGTGATTCTTTCAATAATGCAAATTGAATTTCAGGGTAGATATGAAATTCGAACTTTATCTGGTTTGTTCATGCCAGAGGAAAAGCGCGGAAGGCGGAGTATATCTGGTGGCATGAGTGTTTCTTTAGTGGATCTACATGGACATGTTGTTGGCGGTCGTGTAGCTGGTCCATTGGTGGCTGCAAGTCCTGTAAATGTAAGTACCTGGTCCTGGCTAGGGAATATGTCTTTTTCGCAAACTAAAGTCTAGCAGGATGCTTATGTAGTTATTTATCTTGGATAAGTAAAGTTGATGATTTAGTAACATTATCTGTTTCCTGCTAAAGAAATGGCAACTATATAACAAATTTATGCAATTCTGATAATTTTGTGGTAATTAGTACTAAGTGACAAGTTAATGCAATTCTCATCACTGTTTCCAATGGATTGATATGTATCAGGTAGTGGTAGGCAGTTTTCTGCCAAGTGAACAACATGAGCAGAAGCTAAAAACACAGAATAATGAGGTAATTTCAACTCCAGCTGCTCCCATGTCTACTGCTGGTCCAAATACCTAA
Protein sequence

>MS.gene22562.t1

MEGASNSGASDTPTDNPAAPRTGIPAFADGPTADSTSQGGIPPLQMVAPAKRKRGRPRKYGPDGSLILAIPPKPTSSSIGKAAKFELENPVGAIVNLDPHEGPIEDQTQHPQELEHKVPEGTTFTSRIITVNSGENITMKVMSFCQQGPEAICILSANGVISSATISQPQSSQKLSTYEGKYEIISLSGSFVPNESRSGGMSVSLAGLDGHIVGGCVAGPLVAASPVNVVVSSFLANEQSEQKLKTQENEATSTSMPTADAPVSTAGQLLASSVSATFTPHIFIVNAGEDVSMKIMSFCQQGPEAICILSVNGVISKAVISRPQSSRTLFTYEGRYEIRTLSGLFMPEEKRGRRSISGGMSVSLVDLHGHVVGGRVAGPLVAASPVNVVVGSFLPSEQHEQKLKTQNNEVISTPAAPMSTAGPNT