Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22677.t1 | XP_003600081.1 | 84.8 | 99 | 7 | 2 | 1 | 91 | 1 | 99 | 9.90E-36 | 159.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22677.t1 | G7IXN1 | 84.8 | 99 | 7 | 2 | 1 | 91 | 1 | 99 | 7.1e-36 | 159.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene22677 | 0.828766 | 7.49E-55 | -1.69E-46 |
| MS.gene052534 | MS.gene22677 | 0.822932 | 1.82E-53 | -1.69E-46 |
| MS.gene057208 | MS.gene22677 | 0.805659 | 1.20E-49 | -1.69E-46 |
| MS.gene059440 | MS.gene22677 | 0.803602 | 3.22E-49 | -1.69E-46 |
| MS.gene059584 | MS.gene22677 | 0.816399 | 5.64E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22677.t1 | MTR_3g051530 | 84.848 | 99 | 7 | 2 | 1 | 91 | 1 | 99 | 1.67e-56 | 169 |
| MS.gene22677.t1 | MTR_3g051510 | 85.859 | 99 | 6 | 2 | 1 | 91 | 1 | 99 | 2.54e-55 | 166 |
| MS.gene22677.t1 | MTR_3g051610 | 77.778 | 99 | 12 | 3 | 1 | 91 | 1 | 97 | 7.41e-49 | 150 |
| MS.gene22677.t1 | MTR_3g051570 | 76.238 | 101 | 14 | 3 | 1 | 91 | 1 | 101 | 5.17e-48 | 148 |
| MS.gene22677.t1 | MTR_5g092710 | 54.545 | 88 | 33 | 3 | 1 | 87 | 1 | 82 | 2.45e-26 | 92.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCACCTCGGTTGTCGTTT+TGG | 0.222321 | 3.2:-44054454 | None:intergenic |
| CATTACTATGGTTCATCAAT+TGG | 0.288632 | 3.2:-44054557 | None:intergenic |
| ATCGTCATCGGTCGCATATT+TGG | 0.300232 | 3.2:-44054394 | None:intergenic |
| AATGAAGCTCAATCCAGTTC+AGG | 0.359492 | 3.2:-44054627 | None:intergenic |
| ATCAATCACTTCAAAGATCT+TGG | 0.365689 | 3.2:+44054583 | MS.gene22677:CDS |
| TGACGATGACTGGGATTTCC+AGG | 0.379006 | 3.2:+44054410 | MS.gene22677:CDS |
| GTGATTGATAGCCATTACTA+TGG | 0.397387 | 3.2:-44054569 | None:intergenic |
| ATTACTATGGTTCATCAATT+GGG | 0.423130 | 3.2:-44054556 | None:intergenic |
| ATGCGACCGATGACGATGAC+TGG | 0.438791 | 3.2:+44054400 | MS.gene22677:CDS |
| TGAAATCGCCTTCGGATGCC+TGG | 0.440502 | 3.2:-44054428 | None:intergenic |
| TTTGGATGTGAAATCGCCTT+CGG | 0.465983 | 3.2:-44054436 | None:intergenic |
| TCAGGGATACTTTGAAGTGC+AGG | 0.478577 | 3.2:-44054609 | None:intergenic |
| ATGAAGCTCAATCCAGTTCA+GGG | 0.501390 | 3.2:-44054626 | None:intergenic |
| ACTTCAAAGTATCCCTGAAC+TGG | 0.502693 | 3.2:+44054614 | MS.gene22677:CDS |
| AGTGGACATGAGGGAGTTGA+GGG | 0.505947 | 3.2:+44054518 | MS.gene22677:CDS |
| AGAAGAGTTGTTAAGCAAAG+TGG | 0.524820 | 3.2:+44054500 | MS.gene22677:CDS |
| AAGTGGACATGAGGGAGTTG+AGG | 0.555801 | 3.2:+44054517 | MS.gene22677:CDS |
| CAATTGATGAACCATAGTAA+TGG | 0.571344 | 3.2:+44054558 | MS.gene22677:CDS |
| TGTAATCTTGATCTTCACCT+CGG | 0.575099 | 3.2:-44054466 | None:intergenic |
| TGCGACCGATGACGATGACT+GGG | 0.577074 | 3.2:+44054401 | MS.gene22677:CDS |
| GAAATCCCAGTCATCGTCAT+CGG | 0.597964 | 3.2:-44054406 | None:intergenic |
| TGTTAAGCAAAGTGGACATG+AGG | 0.604541 | 3.2:+44054508 | MS.gene22677:CDS |
| TGGGATTTCCAGGCATCCGA+AGG | 0.607442 | 3.2:+44054420 | MS.gene22677:CDS |
| GTTAAGCAAAGTGGACATGA+GGG | 0.611329 | 3.2:+44054509 | MS.gene22677:CDS |
| GGACATGAGGGAGTTGAGGG+TGG | 0.618214 | 3.2:+44054521 | MS.gene22677:CDS |
| CACATCCAAAACGACAACCG+AGG | 0.703930 | 3.2:+44054449 | MS.gene22677:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTACTATGGTTCATCAATT+GGG | - | chr3.2:44054559-44054578 | None:intergenic | 25.0% |
| ATCAATCACTTCAAAGATCT+TGG | + | chr3.2:44054583-44054602 | MS.gene22677:CDS | 30.0% | |
| CAATTGATGAACCATAGTAA+TGG | + | chr3.2:44054558-44054577 | MS.gene22677:CDS | 30.0% | |
| CATTACTATGGTTCATCAAT+TGG | - | chr3.2:44054560-44054579 | None:intergenic | 30.0% | |
| AGAAGAGTTGTTAAGCAAAG+TGG | + | chr3.2:44054500-44054519 | MS.gene22677:CDS | 35.0% | |
| TGTAATCTTGATCTTCACCT+CGG | - | chr3.2:44054469-44054488 | None:intergenic | 35.0% | |
| ! | GTGATTGATAGCCATTACTA+TGG | - | chr3.2:44054572-44054591 | None:intergenic | 35.0% |
| GTTAAGCAAAGTGGACATGA+GGG | + | chr3.2:44054509-44054528 | MS.gene22677:CDS | 40.0% | |
| TGTTAAGCAAAGTGGACATG+AGG | + | chr3.2:44054508-44054527 | MS.gene22677:CDS | 40.0% | |
| TTTGGATGTGAAATCGCCTT+CGG | - | chr3.2:44054439-44054458 | None:intergenic | 40.0% | |
| ! | ACTTCAAAGTATCCCTGAAC+TGG | + | chr3.2:44054614-44054633 | MS.gene22677:CDS | 40.0% |
| ATCGTCATCGGTCGCATATT+TGG | - | chr3.2:44054397-44054416 | None:intergenic | 45.0% | |
| GAAATCCCAGTCATCGTCAT+CGG | - | chr3.2:44054409-44054428 | None:intergenic | 45.0% | |
| !! | TCAGGGATACTTTGAAGTGC+AGG | - | chr3.2:44054612-44054631 | None:intergenic | 45.0% |
| AAGTGGACATGAGGGAGTTG+AGG | + | chr3.2:44054517-44054536 | MS.gene22677:CDS | 50.0% | |
| AGTGGACATGAGGGAGTTGA+GGG | + | chr3.2:44054518-44054537 | MS.gene22677:CDS | 50.0% | |
| CACATCCAAAACGACAACCG+AGG | + | chr3.2:44054449-44054468 | MS.gene22677:CDS | 50.0% | |
| TGACGATGACTGGGATTTCC+AGG | + | chr3.2:44054410-44054429 | MS.gene22677:CDS | 50.0% | |
| !! | CTTCACCTCGGTTGTCGTTT+TGG | - | chr3.2:44054457-44054476 | None:intergenic | 50.0% |
| ATGCGACCGATGACGATGAC+TGG | + | chr3.2:44054400-44054419 | MS.gene22677:CDS | 55.0% | |
| TGAAATCGCCTTCGGATGCC+TGG | - | chr3.2:44054431-44054450 | None:intergenic | 55.0% | |
| TGCGACCGATGACGATGACT+GGG | + | chr3.2:44054401-44054420 | MS.gene22677:CDS | 55.0% | |
| TGGGATTTCCAGGCATCCGA+AGG | + | chr3.2:44054420-44054439 | MS.gene22677:CDS | 55.0% | |
| GGACATGAGGGAGTTGAGGG+TGG | + | chr3.2:44054521-44054540 | MS.gene22677:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 44054366 | 44054641 | 44054366 | ID=MS.gene22677 |
| chr3.2 | mRNA | 44054366 | 44054641 | 44054366 | ID=MS.gene22677.t1;Parent=MS.gene22677 |
| chr3.2 | exon | 44054366 | 44054641 | 44054366 | ID=MS.gene22677.t1.exon1;Parent=MS.gene22677.t1 |
| chr3.2 | CDS | 44054366 | 44054641 | 44054366 | ID=cds.MS.gene22677.t1;Parent=MS.gene22677.t1 |
| Gene Sequence |
| Protein sequence |
