AlfalfaGEDB | MS.gene22732

M. sativa cultivar XinJiangDaYe / MS.gene22732

KEGG: -
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005509 GO:0005524 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0046872 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564
PFAM: PF00036.35 PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22732.t1	XP_003626588.1	99	596	3	1	1	593	1	596	0.00E+00	1142.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22732.t1	Q9ZUZ2	75.7	600	134	6	1	593	1	595	7.0e-256	884.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22732.t1	G7L528	99.0	596	3	1	1	593	1	596	0.0e+00	1142.1

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050681	MS.gene22732	0.898134	7.14E-77	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene013527	MS.gene22732	PPI
MS.gene22732	MS.gene06853	PPI
MS.gene22732	MS.gene057212	PPI
MS.gene22732	MS.gene06639	PPI
MS.gene22732	MS.gene050086	PPI
MS.gene22732	MS.gene028666	PPI
MS.gene31234	MS.gene22732	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22732.t1	MTR_7g118020	99.329	596	1	1	1	593	1	596	0.0	1220
MS.gene22732.t1	MTR_3g092230	71.993	557	144	6	34	588	38	584	0.0	792
MS.gene22732.t1	MTR_5g017830	61.279	594	202	10	1	582	1	578	0.0	722
MS.gene22732.t1	MTR_7g089480	62.210	561	184	6	31	587	55	591	0.0	713
MS.gene22732.t1	MTR_8g079790	64.353	533	177	7	58	582	45	572	0.0	707
MS.gene22732.t1	MTR_4g086660	66.983	527	166	5	68	591	68	589	0.0	702
MS.gene22732.t1	MTR_5g022030	48.164	463	230	5	131	587	89	547	1.59e-144	430
MS.gene22732.t1	MTR_5g022030	50.711	422	198	5	131	546	89	506	1.71e-141	421
MS.gene22732.t1	MTR_5g089320	37.694	451	258	10	139	580	66	502	5.20e-107	332
MS.gene22732.t1	MTR_0028s0170	37.923	443	255	9	139	572	76	507	3.67e-104	325

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MS.gene22732.t1	AT2G46700	76.159	604	124	8	1	593	1	595	877
MS.gene22732.t1	AT5G24430	65.289	605	187	8	1	593	1	594	771
MS.gene22732.t1	AT3G50530	66.359	541	171	4	58	587	57	597	739
MS.gene22732.t1	AT3G49370	64.474	608	184	8	1	592	1	592	734
MS.gene22732.t1	AT3G50530	62.762	572	171	5	58	587	57	628	724
MS.gene22732.t1	AT2G41140	60.473	592	209	6	1	587	1	572	720
MS.gene22732.t1	AT3G56760	58.992	595	214	8	1	587	1	573	706
MS.gene22732.t1	AT2G46700	81.643	414	76	0	180	593	1	414	696
MS.gene22732.t1	AT2G46700	81.643	414	76	0	180	593	1	414	696
MS.gene22732.t1	AT3G19100	60.915	568	204	6	41	593	35	599	691

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Find 169 sgRNAs with CRISPR-Local

Find 423 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAGGACCAATACCGG+AGG	0.662626	7.4:+642777	MS.gene22732:CDS
AAAATGTTCAAATGCAGCGC+AGG	0.585606	7.4:-648283	None:intergenic
AAACTAACCTTGACAAAATT+CGG	0.369196	7.4:-645269	None:intergenic
AAACTTACTTAGAGCTTGAG+CGG	0.610952	7.4:-646195	None:intergenic
AACACAACCACCATAATTGA+TGG	0.408765	7.4:+642505	MS.gene22732:CDS
AACACCTTGAAGATGACAAA+AGG	0.526305	7.4:-645490	None:intergenic
AACGGTTGAATGACATTGTT+GGG	0.404800	7.4:+645916	MS.gene22732:CDS
AAGAACCCTTGATTCCCTCA+TGG	0.631575	7.4:-647884	None:intergenic
AAGCTATTGAAAGCTCTGTC+AGG	0.505290	7.4:+644763	MS.gene22732:CDS
AAGGCGGCGGAAACGGTCGG+CGG	0.646168	7.4:-642699	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATTGTTCTCTTCTCTTCT+AGG	+	chr7.4:645406-645425	MS.gene22732:intron	30.0%
AAGCAAGAGAGAAATATGTT+TGG	-	chr7.4:648146-648165	None:intergenic	30.0%
AATATATGTGTTAATGCCAG+TGG	+	chr7.4:644093-644112	MS.gene22732:intron	30.0%
AATTAGGATTGTAGATTAGG+CGG	+	chr7.4:647211-647230	MS.gene22732:intron	30.0%
AATTGTTCTCTTCTCTTCTA+GGG	+	chr7.4:645407-645426	MS.gene22732:intron	30.0%
ACACTCACATAAATATGAAC+AGG	-	chr7.4:647754-647773	None:intergenic	30.0%
ACTTCCACATAATAAGATGT+AGG	-	chr7.4:646021-646040	None:intergenic	30.0%
AGACCAACTACTAGTATAAA+AGG	-	chr7.4:645335-645354	None:intergenic	30.0%
AGTCTTGAGTGTGTATATTA+AGG	+	chr7.4:643561-643580	MS.gene22732:intron	30.0%
ATAACAAATATCCAGTTCTC+TGG	-	chr7.4:644914-644933	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	642451	648642	642451	ID=MS.gene22732
chr7.4	mRNA	642451	648642	642451	ID=MS.gene22732.t1;Parent=MS.gene22732
chr7.4	exon	642451	642987	642451	ID=MS.gene22732.t1.exon1;Parent=MS.gene22732.t1
chr7.4	CDS	642451	642987	642451	ID=cds.MS.gene22732.t1;Parent=MS.gene22732.t1
chr7.4	exon	644721	644851	644721	ID=MS.gene22732.t1.exon2;Parent=MS.gene22732.t1
chr7.4	CDS	644721	644851	644721	ID=cds.MS.gene22732.t1;Parent=MS.gene22732.t1
chr7.4	exon	645242	645283	645242	ID=MS.gene22732.t1.exon3;Parent=MS.gene22732.t1
chr7.4	CDS	645242	645283	645242	ID=cds.MS.gene22732.t1;Parent=MS.gene22732.t1
chr7.4	exon	645428	645536	645428	ID=MS.gene22732.t1.exon4;Parent=MS.gene22732.t1
chr7.4	CDS	645428	645536	645428	ID=cds.MS.gene22732.t1;Parent=MS.gene22732.t1
chr7.4	exon	645672	645750	645672	ID=MS.gene22732.t1.exon5;Parent=MS.gene22732.t1
chr7.4	CDS	645672	645750	645672	ID=cds.MS.gene22732.t1;Parent=MS.gene22732.t1
chr7.4	exon	645913	646209	645913	ID=MS.gene22732.t1.exon6;Parent=MS.gene22732.t1
chr7.4	CDS	645913	646209	645913	ID=cds.MS.gene22732.t1;Parent=MS.gene22732.t1
chr7.4	exon	646556	646665	646556	ID=MS.gene22732.t1.exon7;Parent=MS.gene22732.t1
chr7.4	CDS	646556	646665	646556	ID=cds.MS.gene22732.t1;Parent=MS.gene22732.t1
chr7.4	exon	647489	647596	647489	ID=MS.gene22732.t1.exon8;Parent=MS.gene22732.t1
chr7.4	CDS	647489	647596	647489	ID=cds.MS.gene22732.t1;Parent=MS.gene22732.t1
chr7.4	exon	647859	647921	647859	ID=MS.gene22732.t1.exon9;Parent=MS.gene22732.t1
chr7.4	CDS	647859	647921	647859	ID=cds.MS.gene22732.t1;Parent=MS.gene22732.t1
chr7.4	exon	648183	648350	648183	ID=MS.gene22732.t1.exon10;Parent=MS.gene22732.t1
chr7.4	CDS	648183	648350	648183	ID=cds.MS.gene22732.t1;Parent=MS.gene22732.t1
chr7.4	exon	648505	648642	648505	ID=MS.gene22732.t1.exon11;Parent=MS.gene22732.t1
chr7.4	CDS	648505	648642	648505	ID=cds.MS.gene22732.t1;Parent=MS.gene22732.t1

Gene Sequence

>MS.gene22732

ATGGGTCAGTGTTACGGGAAAACCGTTCCCGGCGCCGGCAGCCACCGTCAACAAAACACAACCACCATAATTGATGGTGATGGTGACGGCAGTCGTACTCCTCTACCATCCTTCGCCAACGGAATTCCATCTGTCAAGAACACGCCAGCTCGTTCTTCTTCACACGCCAGTCCATGGCCAAGTCCTTACCCTCACGGTGTCAACCCCAGTCCATCGCCAGCTAGGTCCTCCACGCCCAAAAGATTTTTCCGCCGACCGTTTCCGCCGCCTTCTCCGGCGAAGCATATAAGGGAGTCTCTTGCGAAGAGGCTTGGGAAGGCAAAGCCAAAAGAAGGACCAATACCGGAGGAGCATGGTGTGGAAACGGATTCACAGTCGCTTGATAAGAGCTTTGGGTATAGCAAGAATTTTGGAGCTAAGTATGAGTTAGGGAAAGAAGTTGGACGAGGGCATTTTGGTCATACTTGTTCTGCTAGGGCAAAGAAAGGTGATCTCAAAGATCAACCTGTTGCAGTTAAGATCATTTCCAAGTCCAAGGTTACCTTCTACCTTAATCTTTTTTTTTACTTCAATTCTCTATTTCTATGCATATTCGTTGTTTGGAGGAGTTGATTCTGATTACTTTCGACTAGTCTGATAACATTTGAAAAATTTAGGTTGATTTTATTTGGCCTTTGAGATTTATGAGATTTTTGAATCTATGAGATTTTTAGGCCATTGTTATCTGCCATTGTAGTTTTCGTATGAATTGTTTCTATGTGGAAATTGAATTGTGTGAACTGATTGGAAAGTTGTCTGGCATTGCCTTAAGAAATTTCCTTCCTTTTTGGAGTTTTGATGTTTGTAACGGACATATCGCCTGTGGATTCTAAAATTTATTGATTTTGTCGATTGCTGTCGATTCGTAAAATATCATTGATTTTGTCGGTTGCTGTCGACGCATCAAATATCATTGATTTTGTCGGTTGCTGTCGATATGTTAAATATCATCAAGGGATGTAGACATTTGTGGAACATACTATAATTACTGTTCTTCATTAAAATTGACATCAAGACAATTACATGAGTTGTTTCTTTTTCATTCTTCTTGGAAACATATTTATTTATGAAAGTCTTGAGTGTGTATATTAAGGCTGTCAGAATAATTTGCAGACAAGGCGAAAACTCTTCCGATTTTGTTTAAAATAAAAAGTCATGAAGTATCTCGATTTGTAAGCCCAAGTGGATTGACCTAGTGGCACCACACTAGGTCTGAAGTAGAGGGAATGTGCATAGTAATCTCAATTGGAAAGTCCCTGGTTCAAACTCTGACAAATAAAATTTATGTCATGATGTTTGCACAAGTTTCTCCTTTCCTTCTCCCAAAATACAAAAGGAATGTGCTTGTAGTCCCTAGTGAGAGGCTAAAAACACATTTTGTTCAATGCAGAGTCTCAGGTTGAAGAAAATATAAACAGTGAACAAATCAAAACTTACCACTTGTACAAGATTGAAAAAACATACTTAACCTTTTTTGTCTCAACACACGGAACTTCAAAAGCAGTATCATTTTTTTCAAGTCTTCAGCCTTTTGCAATCCCTAGTATAATTCATAGTGTTTGATTTTAAATATATGTTTTTTTTTTTTTGAATCGGGATTTTAAATATATGTGTTAATGCCAGTGGATACTCTCATGCTATTTTGTCGTTTGCATATTACTCATCTTCTGAATATCTTCAGTTAGTTTCTAGAACCGTTAGGGGAACATATATTTTGTCGTTTGCACGTTGTTTGTCTATTTTATCCCCACGTGCAGAGATACAGTGGTTAAGCTCATTTGTGCTGTAACGGGTTATATGATCGATTCCTTCAGGAGTATCTTTTATTAATTTTCCATTGTTAAAACTTTCTCTGAAAATAGCAAAACGCCTCTTGCTGGAATTGAACTCGTATCATTGCGCCTTGTTTCCAAAAAGATCGTTACCACCGTGCCACAACCAACATTATCAATAATATGACACATAAATTGTACTATAAAATGAAGGAATTTATGCTGTTTGAGCATATCTGTTACCTATTTTTACCCCCTGAAAGGAATTGTTTGAGAATATTACTTAGTTTGTTTATCTCGTACGTTATGAAACATATGTTTGGGAATGTCTTTAACTACATCCTCTATTTGGGCCATAATTAGTCTTATGATATATGCAACTGTAATCATGCTTATTCTTAAGTTTTTCAATATGTTAGATAATTTTTTTGCTGGATCTTATTAAGCTTTATTTGGCAGATGACCTCGGCAATAGCAATTGAAGATGTCCGTAGGGAGGTGAAGCTATTGAAAGCTCTGTCAGGCCATAAGCATCTTATCAAATTTCACGATGCTTGTGAGGATGCCAATAATGTGTACATAGTTATGGAGTATGTCATTCTTTCTTGTTTTCAGAATCTTCTATACTGATACTTTGGTGTAGGGATTGCCAGAGAACTGGATATTTGTTATTTTTAATTTGTACATATTTTTACCTAATGGATGTATTGAACTACAGACTGGGCATTGGTTTTCTGCTTTTTTGTCAGTCTTCGCAATTTATTTCCAGTTTCATAGTTTTTTGTATCTTTGTGCCGTTTCATTGTCAGATTTTTTACATTAGGTTCAATCACAACTTTTTTATACTCTTAAACATTTTTAAGTATCTACTCAAAATATATTTTGATGGAGGATAATTTATGTTTAGTTATAGTGTAACACCCATCTTCTAATCGTGGTATCCTCATATAGTGATGATTATGTTTTTATTTTCTTATCAGATTGTGTGAAGGTGGGGAGCTTCTTGACCGAATTTTGTCAAGGTTAGTTTTTCTGATAACTGTGATTTCCAAAGAGGGTTTTCTTTGGAACCTTTTATACTAGTAGTTGGTCTTTTTTTTTGTGTGGTGGTTTTTAGCTCAATACACTCTTTTATTTTGGTTGAAAATTGTTCTCTTCTCTTCTAGGGGAGGAAGGTATACCGAGGAGGATGCAAAAGCTATTGTTTTGCAGATTCTAAGTGTAGCTGCCTTTTGTCATCTTCAAGGTGTTGTGCATCGTGACTTGAAGCCTGAGGTTTGTGCATTAAATCTTATTCCCCTTCACAACTTGTATCATCTAGCTCTCATTTTAATCTCATGCATGCTTAATTATATATTGATAAGAATGTAAGAGATTTTGATGATTCTTTGTTCGGTTTTGATTTTACAGAATTTCCTCTTCACTTCAAGAAGTGAAGATGCTGATATGAAGCTTATTGATTTTGGTCTCTCTGACTTCATCAGGCCAGGTTATAAATAGAATGATTCTCAATTTTCATTTCCTTGCAAAATTTCATATTTTTCCTCTAAATAGGTTATTTATGACTGCTGCTTCTTTTCATGCATGCTTTATTAGATGTTAACGGATATAAGTAAACTGAACTTTTCCCTTTGTCCGTGCTGATACGCAGAAGAACGGTTGAATGACATTGTTGGGAGTGCGTATTATGTTGCACCTGAGGTTCTTCACAGGTCTTATAGCGTGGAAGCGGATATATGGAGTATTGGTGTTATTACCTACATCTTATTATGTGGAAGTCGACCATTCTGGGCACGAACCGAGTCTGGAATCTTTCGTACAGTTCTCAGAGCTGATCCTAACTTCGATGATTTACCTTGGCCATCTGTGTCTCCAGAGGGAAAGGACTTTGTCAAAAGGCTCCTAAACAAGGACTACAGGAAAAGAATGACTGCCGCTCAAGCTCTAAGTAAGTTTTCCTATCAATTTAGACTAGTATGGCCATTGTTATATGATGCATGATATTTTTTATTGATCATCATGAAATTCTCAATGTGGTATGTCGCATCAAAGAATAATACTTTGACATTATTATAAAAGATTTTTGGTTGTGGAATTTCACGCTTCAAAGTCCTGCCTCAACGGAATGAAGAAAATAGATCAGTATAATGAAAAACTCATTATATTTGTGTTATGGTAGATATCCGAATATTACTCGGTAGGTTCTATTAGGAATAGTCATAGTGGTAGACTAGTGACAGTTTCATTGCAAATTGAATTTTAAAACTAATTAATGCACACTTGTGTGATTGCAGCTCACCCGTGGTTGAGGGATGAAAGTCGTCCGATCCCTTTAGATATATTAATTTATAAGTTGGTGAAGTCTTATCTTCACGCGACACCATTCAAACGTGCAGCTGTGAAGGTATTATTTATTTCATTTCCTAGAATATGCTCACACTGATATGAACTTAGGGGTGTTTATGATATCATTGGTTTAAGAAAATGTGCTATTTATATTACTTGTTTTCACCATTAAATGCAAAACGTTACTTTTGAAAAGAAAAAAAAATATCTGTACTGGAAACAGTGAAAATAATTTCTCACTGATTTTTGTACAATATTTGAAATTAGGATACAAAAATAAAGTAATATCTTTGTATTAAAAGTGAAATATATGAAATGAAAACAGAAAACTTAGAGCCTGTTTAAAACACTTCTCAAAAACTCTTCTTTAGTTTTTTAAAACTTGAAAATTAAAAACTTGTTTGCTAAACTATTTTTAAAATTACAGTTTTGGAAACTGTTTTAAATTTTTTAGTTTTGGGGACTAAAAAACTATAACATGTAAAAGGATAACCATTGTGAAAAGAGCACTTGTATATTTCAAAAACTGAAAACAAAAACTATTTCAAACAGGCCCTTATTCTAGGAATTTTGTTTTCATTTTCAATTTAATATTTCGGCTATAATTAGGATTGTAGATTAGGCGGGGGATAATCCACTAGTTAGAGATAGAAGGAAATGAAGGAGAAGTTAAACCATTTAGAATAATCTGATTTGAATGGTATGTCATTAGAGATGATTAATAAGAAGACATTGTGTTGTCATTTAGATGTGACCGACCCCACCCCCATAATTTGTGCACATTCATTTTATGATTTTGAAATTAGAGGATTGTTTGTCGATATTGATTTATTTATTTACTGCACTGGATCCATACTCATCTTTTCACTTAATTTATCCAGGCCCTTTCGAAAGCATTGACAGACGATCAGTTGGTGTATCTTAGAGCGCAATTCAGATTGTTGGAACCAAATAGAGATGGGCACGTCTCCCTCGACAACTTCAAAATGGTTATCTTTTGCTTCCCTGTTAAATCTTTTGGTCTTTTTATGGTGGACAAAAATTTCTGTGTTTTGTGTTCGTGGATTGTCATTTCATCGGCACAGCTTACTCACTAAGGGGCGCACACACACACATACACTTATACACTTTTTTTTCTTTCTTCCTGTTCATATTTATGTGAGTGTGTTCTTTCCTGTTGAGAGTTTGTGTAAACTTTTGTGACTTTTGACCATTGACCGTTATTAATTAGTTTAATATTGAACTTTGTAGGCTCTTGCACGTCATGCAACTGATGCCATGAGGGAATCAAGGGTTCTTGACATCATACACACGGTTAGCCTTTATTGTTACATAATTTGCATGGACAATTTTTTTATTTTTTTTTAAGGATGCATGTACAAATGTTTATGAGTGCAAGTTTTTCATGAAATTATTCCTATTGAGTCATAACAAGTTACTTATATACCCTAAACTTCTGCTTGTTGTCTTAATATTTTATTGTTTCATCGTTAAACTTATGGGAAACTGATGTACACCTTAGTTCCCTTATTTAACCAAACATATTTCTCTCTTGCTTTGTTAATTAATTTATAGATGGAACCGCTTGCATATAGAAAGATGGATTTTGAAGAATTTTGTGCAGCTGCCACTAGCACATATCAATTGGAGGCACTTGATGGGTGGGAAGATATTGCCTGCGCTGCATTTGAACATTTTGAGAGTGAAGGAAACCGTGTGATATCGATCGAAGAATTAGCAAGAGTAAGTGCTCTAATTTCTCTTCCTAACATAACAATGTACGCCTTTGTTTTCAGGTTTGATTAGGACATACTTTTTTTTTGAGGGAAGGTTTGATTAGGACATACTGTTATGCTGCTCTATGGTTTTTTAAAATATATTATTAATATGGCCACAGGAGCTAAATCTTGGTCCTTCGGCGTATTCAGTTCTCAGAGATTGGATTCGAAATACTGATGGAAAACTTAGTTTACTTGGATATACAAAATTTTTGCATGGCGTGACTCTGCGGAGTTCACTACCAAGACCTCGTTAG

Protein sequence

>MS.gene22732.t1

MGQCYGKTVPGAGSHRQQNTTTIIDGDGDGSRTPLPSFANGIPSVKNTPARSSSHASPWPSPYPHGVNPSPSPARSSTPKRFFRRPFPPPSPAKHIRESLAKRLGKAKPKEGPIPEEHGVETDSQSLDKSFGYSKNFGAKYELGKEVGRGHFGHTCSARAKKGDLKDQPVAVKIISKSKMTSAIAIEDVRREVKLLKALSGHKHLIKFHDACEDANNVYIVMELCEGGELLDRILSRGGRYTEEDAKAIVLQILSVAAFCHLQGVVHRDLKPENFLFTSRSEDADMKLIDFGLSDFIRPEERLNDIVGSAYYVAPEVLHRSYSVEADIWSIGVITYILLCGSRPFWARTESGIFRTVLRADPNFDDLPWPSVSPEGKDFVKRLLNKDYRKRMTAAQALTHPWLRDESRPIPLDILIYKLVKSYLHATPFKRAAVKALSKALTDDQLVYLRAQFRLLEPNRDGHVSLDNFKMALARHATDAMRESRVLDIIHTMEPLAYRKMDFEEFCAAATSTYQLEALDGWEDIACAAFEHFESEGNRVISIEELARELNLGPSAYSVLRDWIRNTDGKLSLLGYTKFLHGVTLRSSLPRPR