Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23483.t1 | XP_013449898.1 | 93.1 | 189 | 13 | 0 | 1 | 189 | 1 | 189 | 5.90E-91 | 343.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23483.t1 | A0A072U3J7 | 93.1 | 189 | 13 | 0 | 1 | 189 | 1 | 189 | 4.2e-91 | 343.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051975 | MS.gene23483 | 0.802492 | 5.47E-49 | -1.69E-46 |
| MS.gene05469 | MS.gene23483 | 0.80728 | 5.46E-50 | -1.69E-46 |
| MS.gene055311 | MS.gene23483 | 0.80442 | 2.18E-49 | -1.69E-46 |
| MS.gene055313 | MS.gene23483 | 0.816469 | 5.44E-52 | -1.69E-46 |
| MS.gene055314 | MS.gene23483 | 0.803278 | 3.76E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23483.t1 | MTR_7g096950 | 93.122 | 189 | 13 | 0 | 1 | 189 | 1 | 189 | 3.78e-131 | 365 |
| MS.gene23483.t1 | MTR_1g042820 | 27.461 | 193 | 117 | 6 | 4 | 187 | 6 | 184 | 3.03e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCTTAGCAGCAAGGAATTT+AGG | 0.230904 | 7.3:-14459311 | None:intergenic |
| TTCGCAGTTATTTCGATTTG+TGG | 0.337470 | 7.3:-14459479 | None:intergenic |
| AGGTGCCTTGGTAGTGATAC+TGG | 0.352156 | 7.3:-14459291 | None:intergenic |
| TCATCAATTCCTTGCATTTG+AGG | 0.408360 | 7.3:-14459614 | None:intergenic |
| TCGATTTGTGGTCTGTGAAT+TGG | 0.422489 | 7.3:-14459467 | None:intergenic |
| AAGAGCTCATCAATATAAAC+AGG | 0.429364 | 7.3:-14459398 | None:intergenic |
| AGCAAGGAATTTAGGTGCCT+TGG | 0.442470 | 7.3:-14459303 | None:intergenic |
| CTCTTCCATACCTTTCTAGC+AGG | 0.462977 | 7.3:-14459167 | None:intergenic |
| TTCTAGCAGGTGGAAGCCTT+AGG | 0.468112 | 7.3:-14459154 | None:intergenic |
| ATCGAAATAACTGCGAAGAT+TGG | 0.468530 | 7.3:+14459485 | MS.gene23483:CDS |
| AAGAGCTTCACTTCTAAACT+TGG | 0.469460 | 7.3:+14459185 | MS.gene23483:CDS |
| CCATGTCTTGCATAAGCCTA+AGG | 0.482076 | 7.3:+14459138 | None:intergenic |
| AAGGCTTCCACCTGCTAGAA+AGG | 0.483331 | 7.3:+14459157 | MS.gene23483:CDS |
| GGTCTGTGAATTGGTGTTTG+TGG | 0.486464 | 7.3:-14459458 | None:intergenic |
| AGAGCCTCCTTGTGACTTAG+TGG | 0.489786 | 7.3:+14459424 | MS.gene23483:CDS |
| TTCCATACCTTTCTAGCAGG+TGG | 0.498563 | 7.3:-14459164 | None:intergenic |
| CAGAAAACAGATGGCAGCGC+AGG | 0.511725 | 7.3:+14459664 | MS.gene23483:CDS |
| TCATCAATATAAACAGGTGC+TGG | 0.515522 | 7.3:-14459392 | None:intergenic |
| CTAGTACATCAGATGATATG+TGG | 0.531445 | 7.3:+14459570 | MS.gene23483:CDS |
| CCTTAGGCTTATGCAAGACA+TGG | 0.565799 | 7.3:-14459138 | None:intergenic |
| TTCCACCTGCTAGAAAGGTA+TGG | 0.580026 | 7.3:+14459162 | MS.gene23483:CDS |
| GCCGAAAACGCTTAGCAGCA+AGG | 0.583139 | 7.3:-14459319 | None:intergenic |
| GAGCCTCCTTGTGACTTAGT+GGG | 0.583807 | 7.3:+14459425 | MS.gene23483:CDS |
| GAGAAGAAAGAAGTGGCAGA+AGG | 0.590916 | 7.3:+14459521 | MS.gene23483:CDS |
| TCAAAGAGAGAAGAAAGAAG+TGG | 0.596495 | 7.3:+14459514 | MS.gene23483:CDS |
| TACCAGGTTCAGAAAACAGA+TGG | 0.601074 | 7.3:+14459655 | MS.gene23483:CDS |
| TTAGCTTCACCTCAAATGCA+AGG | 0.619041 | 7.3:+14459605 | MS.gene23483:CDS |
| TAGTACATCAGATGATATGT+GGG | 0.620935 | 7.3:+14459571 | MS.gene23483:CDS |
| AGGAATTGATGAACGAGCAG+AGG | 0.631742 | 7.3:+14459625 | MS.gene23483:CDS |
| GAGCAGAGGAGTTCATTACC+AGG | 0.638794 | 7.3:+14459639 | MS.gene23483:CDS |
| AGATGATATGTGGGAGTCTG+TGG | 0.644635 | 7.3:+14459580 | MS.gene23483:CDS |
| ATCTACCAGTATCACTACCA+AGG | 0.668685 | 7.3:+14459286 | MS.gene23483:CDS |
| AAATAACCCACTAAGTCACA+AGG | 0.670657 | 7.3:-14459431 | None:intergenic |
| TAACCCACTAAGTCACAAGG+AGG | 0.740102 | 7.3:-14459428 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATGCTTTTTTTGTTTTTTCA+TGG | - | chr7.3:14459243-14459262 | None:intergenic | 20.0% |
| AAGAGCTCATCAATATAAAC+AGG | - | chr7.3:14459401-14459420 | None:intergenic | 30.0% | |
| TAGTACATCAGATGATATGT+GGG | + | chr7.3:14459571-14459590 | MS.gene23483:CDS | 30.0% | |
| ! | GGTAGATGATTTTATAGCTT+TGG | - | chr7.3:14459273-14459292 | None:intergenic | 30.0% |
| AAATAACCCACTAAGTCACA+AGG | - | chr7.3:14459434-14459453 | None:intergenic | 35.0% | |
| AAGAGCTTCACTTCTAAACT+TGG | + | chr7.3:14459185-14459204 | MS.gene23483:CDS | 35.0% | |
| ATCGAAATAACTGCGAAGAT+TGG | + | chr7.3:14459485-14459504 | MS.gene23483:CDS | 35.0% | |
| CTAGTACATCAGATGATATG+TGG | + | chr7.3:14459570-14459589 | MS.gene23483:CDS | 35.0% | |
| TCAAAGAGAGAAGAAAGAAG+TGG | + | chr7.3:14459514-14459533 | MS.gene23483:CDS | 35.0% | |
| TCATCAATATAAACAGGTGC+TGG | - | chr7.3:14459395-14459414 | None:intergenic | 35.0% | |
| TCATCAATTCCTTGCATTTG+AGG | - | chr7.3:14459617-14459636 | None:intergenic | 35.0% | |
| ! | TTCGCAGTTATTTCGATTTG+TGG | - | chr7.3:14459482-14459501 | None:intergenic | 35.0% |
| ATCTACCAGTATCACTACCA+AGG | + | chr7.3:14459286-14459305 | MS.gene23483:CDS | 40.0% | |
| TACCAGGTTCAGAAAACAGA+TGG | + | chr7.3:14459655-14459674 | MS.gene23483:CDS | 40.0% | |
| TTAGCTTCACCTCAAATGCA+AGG | + | chr7.3:14459605-14459624 | MS.gene23483:CDS | 40.0% | |
| ! | TCGATTTGTGGTCTGTGAAT+TGG | - | chr7.3:14459470-14459489 | None:intergenic | 40.0% |
| AGATGATATGTGGGAGTCTG+TGG | + | chr7.3:14459580-14459599 | MS.gene23483:CDS | 45.0% | |
| AGCAAGGAATTTAGGTGCCT+TGG | - | chr7.3:14459306-14459325 | None:intergenic | 45.0% | |
| AGGAATTGATGAACGAGCAG+AGG | + | chr7.3:14459625-14459644 | MS.gene23483:CDS | 45.0% | |
| CGCTTAGCAGCAAGGAATTT+AGG | - | chr7.3:14459314-14459333 | None:intergenic | 45.0% | |
| GAGAAGAAAGAAGTGGCAGA+AGG | + | chr7.3:14459521-14459540 | MS.gene23483:CDS | 45.0% | |
| TAACCCACTAAGTCACAAGG+AGG | - | chr7.3:14459431-14459450 | None:intergenic | 45.0% | |
| TTCCACCTGCTAGAAAGGTA+TGG | + | chr7.3:14459162-14459181 | MS.gene23483:CDS | 45.0% | |
| ! | CTCTTCCATACCTTTCTAGC+AGG | - | chr7.3:14459170-14459189 | None:intergenic | 45.0% |
| ! | GGTCTGTGAATTGGTGTTTG+TGG | - | chr7.3:14459461-14459480 | None:intergenic | 45.0% |
| ! | TCCTTGCTGCTAAGCGTTTT+CGG | + | chr7.3:14459318-14459337 | MS.gene23483:CDS | 45.0% |
| ! | TGCCATCTGTTTTCTGAACC+TGG | - | chr7.3:14459660-14459679 | None:intergenic | 45.0% |
| ! | TTCCATACCTTTCTAGCAGG+TGG | - | chr7.3:14459167-14459186 | None:intergenic | 45.0% |
| AAGGCTTCCACCTGCTAGAA+AGG | + | chr7.3:14459157-14459176 | MS.gene23483:CDS | 50.0% | |
| AGAGCCTCCTTGTGACTTAG+TGG | + | chr7.3:14459424-14459443 | MS.gene23483:CDS | 50.0% | |
| GAGCAGAGGAGTTCATTACC+AGG | + | chr7.3:14459639-14459658 | MS.gene23483:CDS | 50.0% | |
| GAGCCTCCTTGTGACTTAGT+GGG | + | chr7.3:14459425-14459444 | MS.gene23483:CDS | 50.0% | |
| TTCTAGCAGGTGGAAGCCTT+AGG | - | chr7.3:14459157-14459176 | None:intergenic | 50.0% | |
| !! | AGGTGCCTTGGTAGTGATAC+TGG | - | chr7.3:14459294-14459313 | None:intergenic | 50.0% |
| CAGAAAACAGATGGCAGCGC+AGG | + | chr7.3:14459664-14459683 | MS.gene23483:CDS | 55.0% | |
| GCCGAAAACGCTTAGCAGCA+AGG | - | chr7.3:14459322-14459341 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 14459140 | 14459709 | 14459140 | ID=MS.gene23483 |
| chr7.3 | mRNA | 14459140 | 14459709 | 14459140 | ID=MS.gene23483.t1;Parent=MS.gene23483 |
| chr7.3 | exon | 14459140 | 14459709 | 14459140 | ID=MS.gene23483.t1.exon1;Parent=MS.gene23483.t1 |
| chr7.3 | CDS | 14459140 | 14459709 | 14459140 | ID=cds.MS.gene23483.t1;Parent=MS.gene23483.t1 |
| Gene Sequence |
| Protein sequence |
