AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05469


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05469.t1 MTR_6g084600 91.775 231 15 2 1 227 1 231 1.69e-149 415
MS.gene05469.t1 MTR_8g104140 39.474 228 86 10 5 227 6 186 8.56e-27 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05469.t1 AT1G13360 31.100 209 90 8 49 226 8 193 6.51e-11 60.1

Find 63 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGGATGGGTTGTTTGAATA+TGG 0.265419 6.2:+83157369 MS.gene05469:CDS
GACTGAACCGGAACCGGTTC+AGG 0.341085 6.2:+83157089 MS.gene05469:CDS
ATCAACTCTCTTTCTCTTGT+CGG 0.351509 6.2:-83156776 None:intergenic
GATGACTTTGGTTATGGAAC+TGG 0.354739 6.2:+83157303 MS.gene05469:CDS
GCCACCAACTGTCACTGAAC+CGG 0.360188 6.2:+83157197 MS.gene05469:CDS
CTGGTTCAAATGGCCGGTTC+AGG 0.393507 6.2:+83157117 MS.gene05469:CDS
GAGGCTTCTGATGATGAACT+TGG 0.430006 6.2:+83157171 MS.gene05469:CDS
GAACTTAGGTTACTTGTTTG+AGG 0.431948 6.2:+83157152 MS.gene05469:CDS
TGAACCAGGTCGGCCTGAAC+CGG 0.434482 6.2:-83157102 None:intergenic
TCGGAGAGTGATGGCGGGTC+CGG 0.442269 6.2:+83157333 MS.gene05469:CDS
GTCAGACCTGGTTCAGTCCC+AGG 0.443869 6.2:-83157069 None:intergenic
CATCTCCATCTCACCTGAAC+CGG 0.451250 6.2:-83157130 None:intergenic
TCAGGCCGACCTGGTTCAAA+TGG 0.454935 6.2:+83157107 MS.gene05469:CDS
ATCATCAAACCCAACAAAAC+CGG 0.455560 6.2:-83157265 None:intergenic
GGGTTCGAATGAACCGGGTT+CGG 0.457493 6.2:+83157218 MS.gene05469:CDS
CCGGTCAGGTCCACCTTCTC+CGG 0.459201 6.2:-83157246 None:intergenic
AGGTCTGACTGAACCGGAAC+CGG 0.460456 6.2:+83157083 MS.gene05469:CDS
AGGTCGGCCTGAACCGGTTC+CGG 0.461559 6.2:-83157096 None:intergenic
TCAAGACCCATGACTGAGTC+AGG 0.464698 6.2:-83157009 None:intergenic
TGAACCAGGTCTGACTGAAC+CGG 0.472252 6.2:+83157077 MS.gene05469:CDS
TTCTGAGCCTGACTCAGTCA+TGG 0.472858 6.2:+83157002 MS.gene05469:CDS
AGATGAGTTAACATCAGAAA+AGG 0.479022 6.2:-83156863 None:intergenic
TGAACCGGGTTCGGTTGAAC+CGG 0.479989 6.2:+83157227 MS.gene05469:CDS
TGAACCGGGTTCGAATGAAC+CGG 0.486228 6.2:+83157212 MS.gene05469:CDS
TGACTCGAGTGAGTCGAGTC+AGG 0.489034 6.2:+83156918 MS.gene05469:CDS
TTTGCACCTGGGACTGAACC+AGG 0.491336 6.2:+83157063 MS.gene05469:CDS
CCACCAACTGTCACTGAACC+GGG 0.496666 6.2:+83157198 MS.gene05469:CDS
GCCGACCTGGTTCAAATGGC+CGG 0.497769 6.2:+83157111 MS.gene05469:CDS
GGTGAGATGGAGATGAACTT+AGG 0.499586 6.2:+83157138 MS.gene05469:CDS
TCGACTCACTCGAGTCAACT+CGG 0.500752 6.2:-83156912 None:intergenic
ATAACCAAAGTCATCAAAAC+CGG 0.504257 6.2:-83157295 None:intergenic
TTATGGAACTGGCTTGTTGT+CGG 0.511227 6.2:+83157314 MS.gene05469:CDS
AACTCTCTTTCTCTTGTCGG+TGG 0.513345 6.2:-83156773 None:intergenic
AGGCTTCTGATGATGAACTT+GGG 0.517202 6.2:+83157172 MS.gene05469:CDS
GGGTCCGGAGAATTTGTGAC+GGG 0.519177 6.2:+83157348 MS.gene05469:CDS
CCCGGTTCAGTGACAGTTGG+TGG 0.523123 6.2:-83157198 None:intergenic
TCTGAGCCTGACTCAGTCAT+GGG 0.529445 6.2:+83157003 MS.gene05469:CDS
CAGTTGATGCTAAGATTCGT+AGG 0.534819 6.2:+83156833 MS.gene05469:CDS
GTTGTTTGAATATGGTGAAC+CGG 0.543772 6.2:+83157377 MS.gene05469:CDS
CCGGAGAAGGTGGACCTGAC+CGG 0.544040 6.2:+83157246 MS.gene05469:CDS
CACAAAACATCCGCAGCCAC+CGG 0.546264 6.2:-83157396 None:intergenic
AATGGCCGGTTCAGGTGAGA+TGG 0.546414 6.2:+83157125 MS.gene05469:CDS
TTCTCCGGTTCAACCGAACC+CGG 0.546934 6.2:-83157231 None:intergenic
CGACTCACTCGAGTCAACTC+GGG 0.566994 6.2:-83156911 None:intergenic
CTGAACCGGCCATTTGAACC+AGG 0.568351 6.2:-83157116 None:intergenic
TTCGGTTGAACCGGAGAAGG+TGG 0.570994 6.2:+83157236 MS.gene05469:CDS
TGTTGTCGGAGAGTGATGGC+GGG 0.575499 6.2:+83157328 MS.gene05469:CDS
CAAACCCAACAAAACCGGTC+AGG 0.575768 6.2:-83157260 None:intergenic
AGTTGATGCTAAGATTCGTA+GGG 0.588681 6.2:+83156834 MS.gene05469:CDS
ATATGGTGAACCGGTGGCTG+CGG 0.589695 6.2:+83157386 MS.gene05469:CDS
GGGTTCGGTTGAACCGGAGA+AGG 0.602699 6.2:+83157233 MS.gene05469:CDS
GGAACCGGTTCAGGCCGACC+TGG 0.612116 6.2:+83157098 MS.gene05469:CDS
CGGGTCCGGAGAATTTGTGA+CGG 0.628887 6.2:+83157347 MS.gene05469:CDS
TTGTTGTCGGAGAGTGATGG+CGG 0.629129 6.2:+83157327 MS.gene05469:CDS
ACCGGCCATTTGAACCAGGT+CGG 0.632501 6.2:-83157112 None:intergenic
GAACCGGGTTCGAATGAACC+GGG 0.634647 6.2:+83157213 MS.gene05469:CDS
GTCCGGAGAATTTGTGACGG+GGG 0.642674 6.2:+83157350 MS.gene05469:CDS
GGCTTGTTGTCGGAGAGTGA+TGG 0.645676 6.2:+83157324 MS.gene05469:CDS
GAACCCGGTTCAGTGACAGT+TGG 0.648924 6.2:-83157201 None:intergenic
GGTTCCGGTTCAGTCAGACC+TGG 0.663444 6.2:-83157081 None:intergenic
GAACCCGGTTCATTCGAACC+CGG 0.677439 6.2:-83157216 None:intergenic
GGTCCGGAGAATTTGTGACG+GGG 0.680756 6.2:+83157349 MS.gene05469:CDS
GTTTGAATATGGTGAACCGG+TGG 0.685498 6.2:+83157380 MS.gene05469:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGATGAGTTAACATCAGAAA+AGG - chr6.2:83156866-83156885 None:intergenic 30.0%
ATAACCAAAGTCATCAAAAC+CGG - chr6.2:83157298-83157317 None:intergenic 30.0%
ATCAACTCTCTTTCTCTTGT+CGG - chr6.2:83156779-83156798 None:intergenic 35.0%
ATCATCAAACCCAACAAAAC+CGG - chr6.2:83157268-83157287 None:intergenic 35.0%
GAACTTAGGTTACTTGTTTG+AGG + chr6.2:83157152-83157171 MS.gene05469:CDS 35.0%
GTTGTTTGAATATGGTGAAC+CGG + chr6.2:83157377-83157396 MS.gene05469:CDS 35.0%
! AAGAAGAGATTTTTGCACCT+GGG + chr6.2:83157052-83157071 MS.gene05469:CDS 35.0%
! AGTTGATGCTAAGATTCGTA+GGG + chr6.2:83156834-83156853 MS.gene05469:CDS 35.0%
!! GGTTTTGATGACTTTGGTTA+TGG + chr6.2:83157297-83157316 MS.gene05469:CDS 35.0%
!!! GGGTTTGATGATGATTTTAC+CGG + chr6.2:83157276-83157295 MS.gene05469:CDS 35.0%
!!! TTTACCGGTTTTGATGACTT+TGG + chr6.2:83157291-83157310 MS.gene05469:CDS 35.0%
GATGACTTTGGTTATGGAAC+TGG + chr6.2:83157303-83157322 MS.gene05469:CDS 40.0%
! AGGCTTCTGATGATGAACTT+GGG + chr6.2:83157172-83157191 MS.gene05469:CDS 40.0%
! CAGTTGATGCTAAGATTCGT+AGG + chr6.2:83156833-83156852 MS.gene05469:CDS 40.0%
! GAAGAAGAGATTTTTGCACC+TGG + chr6.2:83157051-83157070 MS.gene05469:CDS 40.0%
!! GTCGAGTCAGGTTTTTGATT+CGG + chr6.2:83156930-83156949 MS.gene05469:CDS 40.0%
!! TTATGGAACTGGCTTGTTGT+CGG + chr6.2:83157314-83157333 MS.gene05469:CDS 40.0%
AACTCTCTTTCTCTTGTCGG+TGG - chr6.2:83156776-83156795 None:intergenic 45.0%
GGTGAGATGGAGATGAACTT+AGG + chr6.2:83157138-83157157 MS.gene05469:CDS 45.0%
GTTTGAATATGGTGAACCGG+TGG + chr6.2:83157380-83157399 MS.gene05469:CDS 45.0%
! GAGGCTTCTGATGATGAACT+TGG + chr6.2:83157171-83157190 MS.gene05469:CDS 45.0%
!! GGGGATGGGTTGTTTGAATA+TGG + chr6.2:83157369-83157388 MS.gene05469:CDS 45.0%
ATCCCCCGTCACAAATTCTC+CGG - chr6.2:83157355-83157374 None:intergenic 50.0%
CAAACCCAACAAAACCGGTC+AGG - chr6.2:83157263-83157282 None:intergenic 50.0%
CATCTCCATCTCACCTGAAC+CGG - chr6.2:83157133-83157152 None:intergenic 50.0%
TCAAGACCCATGACTGAGTC+AGG - chr6.2:83157012-83157031 None:intergenic 50.0%
TCGACTCACTCGAGTCAACT+CGG - chr6.2:83156915-83156934 None:intergenic 50.0%
TCTGAGCCTGACTCAGTCAT+GGG + chr6.2:83157003-83157022 MS.gene05469:CDS 50.0%
TGAACCAGGTCTGACTGAAC+CGG + chr6.2:83157077-83157096 MS.gene05469:CDS 50.0%
TGAACCGGGTTCGAATGAAC+CGG + chr6.2:83157212-83157231 MS.gene05469:CDS 50.0%
TTCTGAGCCTGACTCAGTCA+TGG + chr6.2:83157002-83157021 MS.gene05469:CDS 50.0%
TTGTTGTCGGAGAGTGATGG+CGG + chr6.2:83157327-83157346 MS.gene05469:CDS 50.0%
! GAGAATTTGTGACGGGGGAT+GGG + chr6.2:83157355-83157374 MS.gene05469:CDS 50.0%
!!! TGGACCTGACCGGTTTTGTT+GGG + chr6.2:83157256-83157275 MS.gene05469:CDS 50.0%
ACCGGCCATTTGAACCAGGT+CGG - chr6.2:83157115-83157134 None:intergenic 55.0%
AGGTCTGACTGAACCGGAAC+CGG + chr6.2:83157083-83157102 MS.gene05469:CDS 55.0%
ATATGGTGAACCGGTGGCTG+CGG + chr6.2:83157386-83157405 MS.gene05469:CDS 55.0%
CACAAAACATCCGCAGCCAC+CGG - chr6.2:83157399-83157418 None:intergenic 55.0%
CCACCAACTGTCACTGAACC+GGG + chr6.2:83157198-83157217 MS.gene05469:CDS 55.0%
CGACTCACTCGAGTCAACTC+GGG - chr6.2:83156914-83156933 None:intergenic 55.0%
CGGGTCCGGAGAATTTGTGA+CGG + chr6.2:83157347-83157366 MS.gene05469:CDS 55.0%
CTGAACCGGCCATTTGAACC+AGG - chr6.2:83157119-83157138 None:intergenic 55.0%
GAACCCGGTTCAGTGACAGT+TGG - chr6.2:83157204-83157223 None:intergenic 55.0%
GAACCCGGTTCATTCGAACC+CGG - chr6.2:83157219-83157238 None:intergenic 55.0%
GAACCGGGTTCGAATGAACC+GGG + chr6.2:83157213-83157232 MS.gene05469:CDS 55.0%
GCCACCAACTGTCACTGAAC+CGG + chr6.2:83157197-83157216 MS.gene05469:CDS 55.0%
GGGTCCGGAGAATTTGTGAC+GGG + chr6.2:83157348-83157367 MS.gene05469:CDS 55.0%
GGGTTCGAATGAACCGGGTT+CGG + chr6.2:83157218-83157237 MS.gene05469:CDS 55.0%
GGTCCGGAGAATTTGTGACG+GGG + chr6.2:83157349-83157368 MS.gene05469:CDS 55.0%
GTCCGGAGAATTTGTGACGG+GGG + chr6.2:83157350-83157369 MS.gene05469:CDS 55.0%
TCAGGCCGACCTGGTTCAAA+TGG + chr6.2:83157107-83157126 MS.gene05469:CDS 55.0%
TGACTCGAGTGAGTCGAGTC+AGG + chr6.2:83156918-83156937 MS.gene05469:CDS 55.0%
TGTTGTCGGAGAGTGATGGC+GGG + chr6.2:83157328-83157347 MS.gene05469:CDS 55.0%
TTCGGTTGAACCGGAGAAGG+TGG + chr6.2:83157236-83157255 MS.gene05469:CDS 55.0%
TTCTCCGGTTCAACCGAACC+CGG - chr6.2:83157234-83157253 None:intergenic 55.0%
TTTGCACCTGGGACTGAACC+AGG + chr6.2:83157063-83157082 MS.gene05469:CDS 55.0%
! AATGGCCGGTTCAGGTGAGA+TGG + chr6.2:83157125-83157144 MS.gene05469:CDS 55.0%
! CTGGTTCAAATGGCCGGTTC+AGG + chr6.2:83157117-83157136 MS.gene05469:CDS 55.0%
! GGAGAATTTGTGACGGGGGA+TGG + chr6.2:83157354-83157373 MS.gene05469:CDS 55.0%
! TGAACCGGGTTCGGTTGAAC+CGG + chr6.2:83157227-83157246 MS.gene05469:CDS 55.0%
! TGCGGATGTTTTGTGGCGGT+CGG + chr6.2:83157404-83157423 MS.gene05469:CDS 55.0%
! TGGCTGCGGATGTTTTGTGG+CGG + chr6.2:83157400-83157419 MS.gene05469:CDS 55.0%
!! GGCTTGTTGTCGGAGAGTGA+TGG + chr6.2:83157324-83157343 MS.gene05469:CDS 55.0%
!! GTGGACCTGACCGGTTTTGT+TGG + chr6.2:83157255-83157274 MS.gene05469:CDS 55.0%
CCCGGTTCAGTGACAGTTGG+TGG - chr6.2:83157201-83157220 None:intergenic 60.0%
GCCGACCTGGTTCAAATGGC+CGG + chr6.2:83157111-83157130 MS.gene05469:CDS 60.0%
GGTTCCGGTTCAGTCAGACC+TGG - chr6.2:83157084-83157103 None:intergenic 60.0%
GTCAGACCTGGTTCAGTCCC+AGG - chr6.2:83157072-83157091 None:intergenic 60.0%
TGAACCAGGTCGGCCTGAAC+CGG - chr6.2:83157105-83157124 None:intergenic 60.0%
! CGGTGGCTGCGGATGTTTTG+TGG + chr6.2:83157397-83157416 MS.gene05469:CDS 60.0%
! GACTGAACCGGAACCGGTTC+AGG + chr6.2:83157089-83157108 MS.gene05469:CDS 60.0%
! GGGTTCGGTTGAACCGGAGA+AGG + chr6.2:83157233-83157252 MS.gene05469:CDS 60.0%
CCGGAGAAGGTGGACCTGAC+CGG + chr6.2:83157246-83157265 MS.gene05469:CDS 65.0%
CCGGTCAGGTCCACCTTCTC+CGG - chr6.2:83157249-83157268 None:intergenic 65.0%
! AGGTCGGCCTGAACCGGTTC+CGG - chr6.2:83157099-83157118 None:intergenic 65.0%
!! TCGGAGAGTGATGGCGGGTC+CGG + chr6.2:83157333-83157352 MS.gene05469:CDS 65.0%
! GGAACCGGTTCAGGCCGACC+TGG + chr6.2:83157098-83157117 MS.gene05469:CDS 70.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 83156763 83157446 83156763 ID=MS.gene05469
chr6.2 mRNA 83156763 83157446 83156763 ID=MS.gene05469.t1;Parent=MS.gene05469
chr6.2 exon 83156763 83157446 83156763 ID=MS.gene05469.t1.exon1;Parent=MS.gene05469.t1
chr6.2 CDS 83156763 83157446 83156763 ID=cds.MS.gene05469.t1;Parent=MS.gene05469.t1
Gene Sequence

>MS.gene05469

ATGGAGAATTCCACCGACAAGAGAAAGAGAGTTGATGATGAGTTTGAAACTCAAACTCACTGTGTTGACTCAGTTGATGCTAAGATTCGTAGGGTTAACTCCTTTTCTGATGTTAACTCATCTGAAACAAAGACTGAAACTCAGCTTACCCGAGTTGACTCGAGTGAGTCGAGTCAGGTTTTTGATTCGGATGAGATTTTTAACATACTTGATGATGAAAACAATGCTCATGAACGTGATTCTGAGCCTGACTCAGTCATGGGTCTTGACTCAGTTATCAAAAGCTTTGAAGAAGAGATTTTTGCACCTGGGACTGAACCAGGTCTGACTGAACCGGAACCGGTTCAGGCCGACCTGGTTCAAATGGCCGGTTCAGGTGAGATGGAGATGAACTTAGGTTACTTGTTTGAGGCTTCTGATGATGAACTTGGGTTGCCACCAACTGTCACTGAACCGGGTTCGAATGAACCGGGTTCGGTTGAACCGGAGAAGGTGGACCTGACCGGTTTTGTTGGGTTTGATGATGATTTTACCGGTTTTGATGACTTTGGTTATGGAACTGGCTTGTTGTCGGAGAGTGATGGCGGGTCCGGAGAATTTGTGACGGGGGATGGGTTGTTTGAATATGGTGAACCGGTGGCTGCGGATGTTTTGTGGCGGTCGGAGTCTTTACAAGCTATGTAA

Protein sequence

>MS.gene05469.t1

MENSTDKRKRVDDEFETQTHCVDSVDAKIRRVNSFSDVNSSETKTETQLTRVDSSESSQVFDSDEIFNILDDENNAHERDSEPDSVMGLDSVIKSFEEEIFAPGTEPGLTEPEPVQADLVQMAGSGEMEMNLGYLFEASDDELGLPPTVTEPGSNEPGSVEPEKVDLTGFVGFDDDFTGFDDFGYGTGLLSESDGGSGEFVTGDGLFEYGEPVAADVLWRSESLQAM