Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24677.t1 | XP_003590425.1 | 96.6 | 475 | 16 | 0 | 13 | 487 | 17 | 491 | 2.70E-257 | 897.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24677.t1 | Q93ZG7 | 65.9 | 452 | 141 | 6 | 37 | 486 | 54 | 494 | 3.5e-160 | 566.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24677.t1 | G7IA59 | 96.6 | 475 | 16 | 0 | 13 | 487 | 17 | 491 | 2.0e-257 | 897.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050084 | MS.gene24677 | 0.81099 | 8.76E-51 | -1.69E-46 |
| MS.gene050085 | MS.gene24677 | 0.833244 | 5.96E-56 | -1.69E-46 |
| MS.gene050551 | MS.gene24677 | 0.84258 | 2.37E-58 | -1.69E-46 |
| MS.gene051381 | MS.gene24677 | 0.972446 | 8.87E-135 | -1.69E-46 |
| MS.gene051382 | MS.gene24677 | 0.988763 | 2.60E-175 | -1.69E-46 |
| MS.gene052514 | MS.gene24677 | 0.80167 | 8.07E-49 | -1.69E-46 |
| MS.gene052609 | MS.gene24677 | 0.819542 | 1.10E-52 | -1.69E-46 |
| MS.gene053143 | MS.gene24677 | 0.803982 | 2.69E-49 | -1.69E-46 |
| MS.gene056097 | MS.gene24677 | 0.800866 | 1.18E-48 | -1.69E-46 |
| MS.gene057131 | MS.gene24677 | 0.803673 | 3.12E-49 | -1.69E-46 |
| MS.gene059007 | MS.gene24677 | 0.811749 | 5.99E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 108 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGCATTGTTTAGGTTAATT+TGG | 0.187218 | 1.1:+46869703 | MS.gene24677:intron |
| GTTGGTAGGGCTGGGCGATT+TGG | 0.187734 | 1.1:+46869815 | MS.gene24677:CDS |
| TACTAGAGAGTTGGCTATTC+AGG | 0.203921 | 1.1:+46864473 | MS.gene24677:CDS |
| GGCTTGATCGGCCCAGCTTT+TGG | 0.211747 | 1.1:-46863937 | None:intergenic |
| AAAGATTATATATTTGAAAT+AGG | 0.235592 | 1.1:+46869375 | MS.gene24677:CDS |
| TTGCACAGGGTTGGTAGGGC+TGG | 0.257464 | 1.1:+46869806 | MS.gene24677:CDS |
| CATAATCAGGCTCTTGTTCT+TGG | 0.272275 | 1.1:-46869775 | None:intergenic |
| TACAAGGGTTGTTGGGAAAA+AGG | 0.274239 | 1.1:+46869251 | MS.gene24677:CDS |
| CATCCCTTTCACCACATATC+AGG | 0.299190 | 1.1:-46870890 | None:intergenic |
| CACAAAGCTCTTGTTGATTT+AGG | 0.301798 | 1.1:+46869456 | MS.gene24677:CDS |
| ATTCAATTCCGCCATCAAAA+AGG | 0.310135 | 1.1:+46865416 | MS.gene24677:CDS |
| ATAACAACCTGCGCCATAAT+AGG | 0.316481 | 1.1:-46865442 | None:intergenic |
| TTGGTAGGGCTGGGCGATTT+GGG | 0.326658 | 1.1:+46869816 | MS.gene24677:CDS |
| ATTATGGCGCAGGTTGTTAT+TGG | 0.328812 | 1.1:+46865445 | MS.gene24677:CDS |
| GCTCAAGCACATAATGGTTC+TGG | 0.328987 | 1.1:+46864369 | MS.gene24677:CDS |
| CGCTGTAATGTTGGTGTCGT+CGG | 0.335543 | 1.1:-46864075 | None:intergenic |
| TTAATCTTGGGATGCTTAGT+AGG | 0.349931 | 1.1:+46864406 | MS.gene24677:CDS |
| GCTTGAGGAGCTTGCAGATT+GGG | 0.351974 | 1.1:-46864435 | None:intergenic |
| GGCGGAGGTGGAGGTTTGAT+TGG | 0.363101 | 1.1:-46863970 | None:intergenic |
| GGTTTCGTCGGCGGCGGAGG+TGG | 0.370257 | 1.1:-46863982 | None:intergenic |
| GTCGGGATCATCTAGAGATT+TGG | 0.370263 | 1.1:-46864057 | None:intergenic |
| TGCAAATACACCTCATAATC+AGG | 0.379608 | 1.1:-46869788 | None:intergenic |
| AGAGGTTTCGTCGGCGGCGG+AGG | 0.385106 | 1.1:-46863985 | None:intergenic |
| TGCACAGGGTTGGTAGGGCT+GGG | 0.404676 | 1.1:+46869807 | MS.gene24677:CDS |
| TGGCAGTGGAGGAGAGGTGG+TGG | 0.404758 | 1.1:-46863913 | None:intergenic |
| TTCGTCGGCGGCGGAGGTGG+AGG | 0.405769 | 1.1:-46863979 | None:intergenic |
| CTCCACTGCCATCCAAAAGC+TGG | 0.412253 | 1.1:+46863925 | MS.gene24677:CDS |
| CTCAGAATGTGCAGTTCCTA+TGG | 0.418718 | 1.1:+46865384 | MS.gene24677:CDS |
| TTGATAGCTCAAGCACATAA+TGG | 0.420456 | 1.1:+46864363 | MS.gene24677:CDS |
| CAGGTTGTTATTGGTACTCC+TGG | 0.424362 | 1.1:+46865454 | MS.gene24677:CDS |
| GGCTGGGCGATTTGGGCGCA+AGG | 0.424800 | 1.1:+46869823 | MS.gene24677:CDS |
| ATCAAATTCTTAATTGTGCC+AGG | 0.425276 | 1.1:-46865472 | None:intergenic |
| GCTGTAATGTTGGTGTCGTC+GGG | 0.427170 | 1.1:-46864074 | None:intergenic |
| GAGATTCAATTCAAGCTGTC+AGG | 0.429705 | 1.1:+46867104 | MS.gene24677:CDS |
| CCGTGGCTTTGATCAGCAAC+AGG | 0.433702 | 1.1:+46869590 | MS.gene24677:CDS |
| CAGGCTCTTGTTCTTGGCCA+CGG | 0.435431 | 1.1:-46869769 | None:intergenic |
| AAAGTTGCTCTTAAAGAAGC+TGG | 0.440458 | 1.1:+46871120 | MS.gene24677:CDS |
| AACTTCGTGGCGGATGTGTA+AGG | 0.443398 | 1.1:-46864219 | None:intergenic |
| GGTTTCTTCGTCGGCTTGAT+CGG | 0.446945 | 1.1:-46863949 | None:intergenic |
| TGTATTTGCACAGGGTTGGT+AGG | 0.446992 | 1.1:+46869801 | MS.gene24677:CDS |
| TTGATTACTTATAAGAAACT+TGG | 0.451832 | 1.1:+46865490 | MS.gene24677:CDS |
| ATGGTCAAGAGCACCTTGTA+TGG | 0.458366 | 1.1:-46869493 | None:intergenic |
| GGATGGCAGTGGAGGAGAGG+TGG | 0.468980 | 1.1:-46863916 | None:intergenic |
| GGTTTGATTGGTTTCTTCGT+CGG | 0.469217 | 1.1:-46863958 | None:intergenic |
| CATTGAGGTTCTCCGTAAGA+TGG | 0.469249 | 1.1:+46865342 | MS.gene24677:CDS |
| TCAATCTTAGCAAGCTCGTC+AGG | 0.469456 | 1.1:-46869345 | None:intergenic |
| GTATTTGCACAGGGTTGGTA+GGG | 0.470296 | 1.1:+46869802 | MS.gene24677:CDS |
| TTCATTGACGTTGAGGGAAG+AGG | 0.471693 | 1.1:-46864003 | None:intergenic |
| GGAAGAGGTTTCGTCGGCGG+CGG | 0.471697 | 1.1:-46863988 | None:intergenic |
| TGAATAGCCAACTCTCTAGT+AGG | 0.472110 | 1.1:-46864471 | None:intergenic |
| AGGCTCTTGTTCTTGGCCAC+GGG | 0.481618 | 1.1:-46869768 | None:intergenic |
| ATCGTCAAAGAGTTCAAAGA+TGG | 0.487343 | 1.1:+46869531 | MS.gene24677:CDS |
| ATTGAGGTTCTCCGTAAGAT+GGG | 0.491851 | 1.1:+46865343 | MS.gene24677:CDS |
| TAATCTTGGGATGCTTAGTA+GGG | 0.492378 | 1.1:+46864407 | MS.gene24677:CDS |
| CCGTGTACTTCCCCATCTTA+CGG | 0.493677 | 1.1:-46865354 | None:intergenic |
| ATTATGAGGTGTATTTGCAC+AGG | 0.499481 | 1.1:+46869792 | MS.gene24677:CDS |
| ATGTGGAGGATTAAGAATCA+TGG | 0.501190 | 1.1:-46864334 | None:intergenic |
| TCCACTGCCATCCAAAAGCT+GGG | 0.503491 | 1.1:+46863926 | MS.gene24677:CDS |
| CGTAAGATGGGGAAGTACAC+GGG | 0.512273 | 1.1:+46865355 | MS.gene24677:CDS |
| TCTTGTTGATTTAGGCTATG+AGG | 0.514061 | 1.1:+46869464 | MS.gene24677:CDS |
| GATTCTTAATCCTCCACATA+GGG | 0.514959 | 1.1:+46864338 | MS.gene24677:CDS |
| AGCTTGAGGAGCTTGCAGAT+TGG | 0.515271 | 1.1:-46864436 | None:intergenic |
| GGTTAATTCATTGACGTTGA+GGG | 0.525067 | 1.1:-46864009 | None:intergenic |
| TATAATCCCTTCAAAAGCTC+CGG | 0.526079 | 1.1:-46864261 | None:intergenic |
| GATCATCTAGAGATTTGGAA+TGG | 0.526531 | 1.1:-46864052 | None:intergenic |
| GTTCAAAGATGGTTTGACTC+AGG | 0.527101 | 1.1:+46869542 | MS.gene24677:CDS |
| ATCATCTAGAGATTTGGAAT+GGG | 0.527480 | 1.1:-46864051 | None:intergenic |
| AGAACAAGAGCCTGATTATG+AGG | 0.530550 | 1.1:+46869778 | MS.gene24677:CDS |
| TAACCTGATATGTGGTGAAA+GGG | 0.534784 | 1.1:+46870887 | MS.gene24677:CDS |
| TATGAGGTCACTTCCATACA+AGG | 0.536146 | 1.1:+46869480 | MS.gene24677:CDS |
| ATTTGAAATAGGAGAGAATG+TGG | 0.540066 | 1.1:+46869386 | MS.gene24677:CDS |
| TATTTGTCCTACTAGAGAGT+TGG | 0.540350 | 1.1:+46864464 | MS.gene24677:CDS |
| TGATAGCTCTTCGAACTTCG+TGG | 0.543747 | 1.1:-46864232 | None:intergenic |
| TTAACCTGATATGTGGTGAA+AGG | 0.547238 | 1.1:+46870886 | MS.gene24677:CDS |
| TGATTCTTAATCCTCCACAT+AGG | 0.547639 | 1.1:+46864337 | MS.gene24677:CDS |
| CCGTAAGATGGGGAAGTACA+CGG | 0.548975 | 1.1:+46865354 | MS.gene24677:CDS |
| CGATCTTGTCTCTATCATCA+TGG | 0.553290 | 1.1:-46869512 | None:intergenic |
| TCGACTGATGTTCTTGCCCG+TGG | 0.555694 | 1.1:+46869573 | MS.gene24677:CDS |
| TGTGGAGGATTAAGAATCAT+GGG | 0.556922 | 1.1:-46864333 | None:intergenic |
| TGGTTAATTCATTGACGTTG+AGG | 0.557741 | 1.1:-46864010 | None:intergenic |
| GAGGGAAGAGGTTTCGTCGG+CGG | 0.558959 | 1.1:-46863991 | None:intergenic |
| GTGCAGTTCCTATGGACAGA+AGG | 0.559745 | 1.1:+46865392 | MS.gene24677:CDS |
| ATTGAATCCCTTCTGTCCAT+AGG | 0.561511 | 1.1:-46865400 | None:intergenic |
| GGACAAATACATAGAGCTTG+AGG | 0.569078 | 1.1:-46864450 | None:intergenic |
| AGTGGAGGAGAGGTGGTGGA+AGG | 0.578866 | 1.1:-46863909 | None:intergenic |
| GAGGTGTATTTGCACAGGGT+TGG | 0.581567 | 1.1:+46869797 | MS.gene24677:CDS |
| CCTGTTGCTGATCAAAGCCA+CGG | 0.583710 | 1.1:-46869590 | None:intergenic |
| TTTGGATGGCAGTGGAGGAG+AGG | 0.584058 | 1.1:-46863919 | None:intergenic |
| TTTGAAATAGGAGAGAATGT+GGG | 0.584556 | 1.1:+46869387 | MS.gene24677:CDS |
| AAGGGCGCCTATTATGGCGC+AGG | 0.587444 | 1.1:+46865435 | MS.gene24677:CDS |
| TGCAGTTCCTATGGACAGAA+GGG | 0.587722 | 1.1:+46865393 | MS.gene24677:CDS |
| GCTGGGCGATTTGGGCGCAA+GGG | 0.595158 | 1.1:+46869824 | MS.gene24677:CDS |
| TTGAAATAGGAGAGAATGTG+GGG | 0.596292 | 1.1:+46869388 | MS.gene24677:CDS |
| GTTGAGGGAAGAGGTTTCGT+CGG | 0.598306 | 1.1:-46863994 | None:intergenic |
| CTGTTGCTGATCAAAGCCAC+GGG | 0.620873 | 1.1:-46869589 | None:intergenic |
| ATGACGAACCGCTGTAATGT+TGG | 0.631036 | 1.1:-46864084 | None:intergenic |
| TACTCAGCAGAGTACTTCAG+TGG | 0.636769 | 1.1:-46869743 | None:intergenic |
| TGAGCTATCAAATCCCTATG+TGG | 0.642603 | 1.1:-46864351 | None:intergenic |
| GCTGTATTTAACCTGATATG+TGG | 0.649135 | 1.1:+46870879 | MS.gene24677:CDS |
| TTATGAGGTGTATTTGCACA+GGG | 0.652424 | 1.1:+46869793 | MS.gene24677:CDS |
| GGCGGATCAAATGCTTGCTG+AGG | 0.654171 | 1.1:+46865546 | MS.gene24677:CDS |
| TTGAGGTTCTCCGTAAGATG+GGG | 0.657518 | 1.1:+46865344 | MS.gene24677:CDS |
| CGAAGAGCTATCACTCTCGC+CGG | 0.659142 | 1.1:+46864242 | MS.gene24677:CDS |
| GCTATCAAATCCCTATGTGG+AGG | 0.660049 | 1.1:-46864348 | None:intergenic |
| TACTCTGCTGAGTACACCCG+TGG | 0.681522 | 1.1:+46869752 | MS.gene24677:CDS |
| GTAAGATGGGGAAGTACACG+GGG | 0.699517 | 1.1:+46865356 | MS.gene24677:CDS |
| TAGCTCTTCGAACTTCGTGG+CGG | 0.715857 | 1.1:-46864229 | None:intergenic |
| CGACGACACCAACATTACAG+CGG | 0.774689 | 1.1:+46864076 | MS.gene24677:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 46863907 | 46871155 | 46863907 | ID=MS.gene24677 |
| chr1.1 | mRNA | 46863907 | 46871155 | 46863907 | ID=MS.gene24677.t1;Parent=MS.gene24677 |
| chr1.1 | exon | 46863907 | 46864097 | 46863907 | ID=MS.gene24677.t1.exon1;Parent=MS.gene24677.t1 |
| chr1.1 | CDS | 46863907 | 46864097 | 46863907 | ID=cds.MS.gene24677.t1;Parent=MS.gene24677.t1 |
| chr1.1 | exon | 46864201 | 46864494 | 46864201 | ID=MS.gene24677.t1.exon2;Parent=MS.gene24677.t1 |
| chr1.1 | CDS | 46864201 | 46864494 | 46864201 | ID=cds.MS.gene24677.t1;Parent=MS.gene24677.t1 |
| chr1.1 | exon | 46865340 | 46865567 | 46865340 | ID=MS.gene24677.t1.exon3;Parent=MS.gene24677.t1 |
| chr1.1 | CDS | 46865340 | 46865567 | 46865340 | ID=cds.MS.gene24677.t1;Parent=MS.gene24677.t1 |
| chr1.1 | exon | 46867060 | 46867125 | 46867060 | ID=MS.gene24677.t1.exon4;Parent=MS.gene24677.t1 |
| chr1.1 | CDS | 46867060 | 46867125 | 46867060 | ID=cds.MS.gene24677.t1;Parent=MS.gene24677.t1 |
| chr1.1 | exon | 46869204 | 46869611 | 46869204 | ID=MS.gene24677.t1.exon5;Parent=MS.gene24677.t1 |
| chr1.1 | CDS | 46869204 | 46869611 | 46869204 | ID=cds.MS.gene24677.t1;Parent=MS.gene24677.t1 |
| chr1.1 | exon | 46869716 | 46869845 | 46869716 | ID=MS.gene24677.t1.exon6;Parent=MS.gene24677.t1 |
| chr1.1 | CDS | 46869716 | 46869845 | 46869716 | ID=cds.MS.gene24677.t1;Parent=MS.gene24677.t1 |
| chr1.1 | exon | 46870877 | 46870962 | 46870877 | ID=MS.gene24677.t1.exon7;Parent=MS.gene24677.t1 |
| chr1.1 | CDS | 46870877 | 46870962 | 46870877 | ID=cds.MS.gene24677.t1;Parent=MS.gene24677.t1 |
| chr1.1 | exon | 46871093 | 46871155 | 46871093 | ID=MS.gene24677.t1.exon8;Parent=MS.gene24677.t1 |
| chr1.1 | CDS | 46871093 | 46871155 | 46871093 | ID=cds.MS.gene24677.t1;Parent=MS.gene24677.t1 |
| Gene Sequence |
| Protein sequence |
