Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24781.t1 | XP_019418075.1 | 69.6 | 112 | 31 | 1 | 1 | 112 | 1 | 109 | 2.10E-35 | 158.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24781.t1 | Q9LUV2 | 62.5 | 112 | 39 | 1 | 1 | 112 | 1 | 109 | 4.1e-32 | 138.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24781.t1 | A0A151TDN5 | 67.9 | 112 | 33 | 1 | 1 | 112 | 1 | 109 | 7.4e-35 | 156.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050811 | MS.gene24781 | -0.81046 | 1.14E-50 | -1.69E-46 |
| MS.gene054343 | MS.gene24781 | 0.901407 | 2.76E-78 | -1.69E-46 |
| MS.gene054344 | MS.gene24781 | 0.830419 | 2.97E-55 | -1.69E-46 |
| MS.gene056351 | MS.gene24781 | 0.827163 | 1.82E-54 | -1.69E-46 |
| MS.gene057196 | MS.gene24781 | 0.815295 | 9.95E-52 | -1.69E-46 |
| MS.gene057332 | MS.gene24781 | 0.801128 | 1.04E-48 | -1.69E-46 |
| MS.gene059512 | MS.gene24781 | 0.829215 | 5.83E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24781.t1 | MTR_4g124920 | 76.190 | 105 | 22 | 1 | 8 | 112 | 3 | 104 | 1.23e-50 | 155 |
| MS.gene24781.t1 | MTR_4g124920 | 75.000 | 72 | 15 | 1 | 8 | 79 | 3 | 71 | 2.58e-30 | 103 |
| MS.gene24781.t1 | MTR_2g084985 | 36.893 | 103 | 60 | 2 | 9 | 111 | 8 | 105 | 1.77e-14 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24781.t1 | AT3G17210 | 62.500 | 112 | 39 | 1 | 1 | 112 | 1 | 109 | 2.50e-45 | 142 |
| MS.gene24781.t1 | AT3G17210 | 62.500 | 112 | 39 | 1 | 1 | 112 | 1 | 109 | 2.50e-45 | 142 |
| MS.gene24781.t1 | AT5G22580 | 34.021 | 97 | 59 | 2 | 9 | 105 | 7 | 98 | 8.25e-15 | 65.5 |
Find 25 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGTTCCTCAATCTTCTCT+TGG | 0.329948 | 8.2:-7934114 | None:intergenic |
| GATTCAGGTAACAAATAATA+AGG | 0.348275 | 8.2:-7934432 | None:intergenic |
| AATGAGTAAATCCTTGATTC+AGG | 0.352373 | 8.2:-7934447 | None:intergenic |
| TTACCAATGGAATGACTTCA+TGG | 0.420824 | 8.2:-7934173 | None:intergenic |
| ATTATTTGTTACCTGAATCA+AGG | 0.432767 | 8.2:+7934436 | MS.gene24781:CDS |
| AAAGTTGCTCGTTCCTTGCT+TGG | 0.439365 | 8.2:+7934543 | MS.gene24781:CDS |
| GAGTTCCTCAATCTTCTCTT+GGG | 0.443255 | 8.2:-7934113 | None:intergenic |
| GTAGTGAAGAACATAGTGAT+TGG | 0.457715 | 8.2:+7934070 | MS.gene24781:CDS |
| GAGGAAATGGAGGAAGCAAA+AGG | 0.502851 | 8.2:+7934046 | None:intergenic |
| TGGGATGAGATTGACGAGAT+TGG | 0.516286 | 8.2:-7934149 | None:intergenic |
| ATTCAAAGGTTAACGATGGT+GGG | 0.545431 | 8.2:-7934592 | None:intergenic |
| TCCTTGCTTGGAGAAAGTGC+TGG | 0.548785 | 8.2:+7934555 | MS.gene24781:CDS |
| CACCCATGAAGTCATTCCAT+TGG | 0.553232 | 8.2:+7934170 | MS.gene24781:CDS |
| TGAATCAACATTTGAGAGTG+TGG | 0.555452 | 8.2:+7934474 | MS.gene24781:CDS |
| TTTGCATACTCAACATGAGC+AGG | 0.558557 | 8.2:-7934523 | None:intergenic |
| GAAGATTGAGGAACTCATCA+AGG | 0.571882 | 8.2:+7934120 | MS.gene24781:CDS |
| GCCAGCACTTTCTCCAAGCA+AGG | 0.576667 | 8.2:-7934556 | None:intergenic |
| GATTCAAAGGTTAACGATGG+TGG | 0.578082 | 8.2:-7934593 | None:intergenic |
| GAGATTGACGAGATTGGCAA+AGG | 0.580515 | 8.2:-7934143 | None:intergenic |
| CAATGGAATGACTTCATGGG+TGG | 0.583790 | 8.2:-7934169 | None:intergenic |
| AATCTCCCAAGAGAAGATTG+AGG | 0.597731 | 8.2:+7934108 | MS.gene24781:CDS |
| ATGAGCTACATACTCTGCAA+TGG | 0.614733 | 8.2:-7934500 | None:intergenic |
| TACCAATGGAATGACTTCAT+GGG | 0.631729 | 8.2:-7934172 | None:intergenic |
| AATGGAATGACTTCATGGGT+GGG | 0.706119 | 8.2:-7934168 | None:intergenic |
| ACGGATTCAAAGGTTAACGA+TGG | 0.711712 | 8.2:-7934596 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTAAGTTTTTTAAGTTT+TGG | - | chr8.2:7934275-7934294 | None:intergenic | 10.0% |
| !!! | TATACATGTCTTATTTTTTT+AGG | + | chr8.2:7934392-7934411 | MS.gene24781:intron | 15.0% |
| ! | GATTCAGGTAACAAATAATA+AGG | - | chr8.2:7934435-7934454 | None:intergenic | 25.0% |
| !! | ATTATTTGTTACCTGAATCA+AGG | + | chr8.2:7934436-7934455 | MS.gene24781:CDS | 25.0% |
| !! | TTTAAGTTTCATCTTACCAA+TGG | - | chr8.2:7934189-7934208 | None:intergenic | 25.0% |
| AATGAGTAAATCCTTGATTC+AGG | - | chr8.2:7934450-7934469 | None:intergenic | 30.0% | |
| GTAGTGAAGAACATAGTGAT+TGG | + | chr8.2:7934070-7934089 | MS.gene24781:CDS | 35.0% | |
| TACCAATGGAATGACTTCAT+GGG | - | chr8.2:7934175-7934194 | None:intergenic | 35.0% | |
| TGAATCAACATTTGAGAGTG+TGG | + | chr8.2:7934474-7934493 | MS.gene24781:CDS | 35.0% | |
| TTACCAATGGAATGACTTCA+TGG | - | chr8.2:7934176-7934195 | None:intergenic | 35.0% | |
| !! | ATAGTCTAGACTGCTTTTGT+CGG | - | chr8.2:7934225-7934244 | None:intergenic | 35.0% |
| AATCTCCCAAGAGAAGATTG+AGG | + | chr8.2:7934108-7934127 | MS.gene24781:CDS | 40.0% | |
| AATGGAATGACTTCATGGGT+GGG | - | chr8.2:7934171-7934190 | None:intergenic | 40.0% | |
| ATGAGCTACATACTCTGCAA+TGG | - | chr8.2:7934503-7934522 | None:intergenic | 40.0% | |
| GAAGATTGAGGAACTCATCA+AGG | + | chr8.2:7934120-7934139 | MS.gene24781:CDS | 40.0% | |
| GAGTTCCTCAATCTTCTCTT+GGG | - | chr8.2:7934116-7934135 | None:intergenic | 40.0% | |
| TGAGTTCCTCAATCTTCTCT+TGG | - | chr8.2:7934117-7934136 | None:intergenic | 40.0% | |
| TTTGCATACTCAACATGAGC+AGG | - | chr8.2:7934526-7934545 | None:intergenic | 40.0% | |
| AAAGTTGCTCGTTCCTTGCT+TGG | + | chr8.2:7934543-7934562 | MS.gene24781:CDS | 45.0% | |
| CAATGGAATGACTTCATGGG+TGG | - | chr8.2:7934172-7934191 | None:intergenic | 45.0% | |
| CACCCATGAAGTCATTCCAT+TGG | + | chr8.2:7934170-7934189 | MS.gene24781:CDS | 45.0% | |
| GAGATTGACGAGATTGGCAA+AGG | - | chr8.2:7934146-7934165 | None:intergenic | 45.0% | |
| TGGGATGAGATTGACGAGAT+TGG | - | chr8.2:7934152-7934171 | None:intergenic | 45.0% | |
| ! | GATTTTCGCTAAGTGGACGA+GGG | - | chr8.2:7934311-7934330 | None:intergenic | 45.0% |
| ! | TACCAGCGATTTTCGCTAAG+TGG | - | chr8.2:7934318-7934337 | None:intergenic | 45.0% |
| GTCCACTTAGCGAAAATCGC+TGG | + | chr8.2:7934313-7934332 | MS.gene24781:intron | 50.0% | |
| ! | CGATTTTCGCTAAGTGGACG+AGG | - | chr8.2:7934312-7934331 | None:intergenic | 50.0% |
| ! | TCCTTGCTTGGAGAAAGTGC+TGG | + | chr8.2:7934555-7934574 | MS.gene24781:CDS | 50.0% |
| GCCAGCACTTTCTCCAAGCA+AGG | - | chr8.2:7934559-7934578 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 7934052 | 7934612 | 7934052 | ID=MS.gene24781 |
| chr8.2 | mRNA | 7934052 | 7934612 | 7934052 | ID=MS.gene24781.t1;Parent=MS.gene24781 |
| chr8.2 | exon | 7934052 | 7934191 | 7934052 | ID=MS.gene24781.t1.exon1;Parent=MS.gene24781.t1 |
| chr8.2 | CDS | 7934052 | 7934191 | 7934052 | ID=cds.MS.gene24781.t1;Parent=MS.gene24781.t1 |
| chr8.2 | exon | 7934414 | 7934612 | 7934414 | ID=MS.gene24781.t1.exon2;Parent=MS.gene24781.t1 |
| chr8.2 | CDS | 7934414 | 7934612 | 7934414 | ID=cds.MS.gene24781.t1;Parent=MS.gene24781.t1 |
| Gene Sequence |
| Protein sequence |
