Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25083.t1 | XP_003627365.1 | 93.3 | 165 | 2 | 1 | 1 | 156 | 1 | 165 | 1.10E-77 | 299.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25083.t1 | Q94AU2 | 82.4 | 165 | 20 | 1 | 1 | 156 | 1 | 165 | 2.6e-72 | 272.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25083.t1 | G7L8C0 | 93.3 | 165 | 2 | 1 | 1 | 156 | 1 | 165 | 7.6e-78 | 299.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050085 | MS.gene25083 | 0.811187 | 7.94E-51 | -1.69E-46 |
| MS.gene050114 | MS.gene25083 | 0.804052 | 2.60E-49 | -1.69E-46 |
| MS.gene050359 | MS.gene25083 | 0.805185 | 1.51E-49 | -1.69E-46 |
| MS.gene051184 | MS.gene25083 | 0.800052 | 1.73E-48 | -1.69E-46 |
| MS.gene051343 | MS.gene25083 | 0.803681 | 3.10E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene25083 | 0.800144 | 1.65E-48 | -1.69E-46 |
| MS.gene052242 | MS.gene25083 | -0.824929 | 6.18E-54 | -1.69E-46 |
| MS.gene056849 | MS.gene25083 | 0.80737 | 5.23E-50 | -1.69E-46 |
| MS.gene058638 | MS.gene25083 | 0.803947 | 2.73E-49 | -1.69E-46 |
| MS.gene058639 | MS.gene25083 | 0.808982 | 2.37E-50 | -1.69E-46 |
| MS.gene06010 | MS.gene25083 | 0.810141 | 1.34E-50 | -1.69E-46 |
| MS.gene060563 | MS.gene25083 | 0.82504 | 5.82E-54 | -1.69E-46 |
| MS.gene060869 | MS.gene25083 | 0.806349 | 8.59E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25083.t1 | MTR_8g022210 | 93.333 | 165 | 2 | 1 | 1 | 156 | 1 | 165 | 2.14e-111 | 315 |
| MS.gene25083.t1 | MTR_1g100663 | 36.250 | 160 | 90 | 3 | 1 | 149 | 1 | 159 | 9.73e-31 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25083.t1 | AT1G11890 | 82.424 | 165 | 20 | 1 | 1 | 156 | 1 | 165 | 7.33e-100 | 286 |
| MS.gene25083.t1 | AT5G52270 | 37.063 | 143 | 78 | 3 | 1 | 131 | 1 | 143 | 3.30e-26 | 99.4 |
| MS.gene25083.t1 | AT5G52270 | 37.063 | 143 | 78 | 3 | 1 | 131 | 1 | 143 | 3.51e-26 | 99.4 |
Find 37 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGAATTTGAGCGTGTTAA+TGG | 0.238804 | 8.4:-72192132 | MS.gene25083:CDS |
| CCTTATGCCTTCATTAAGTT+TGG | 0.298992 | 8.4:-72192084 | MS.gene25083:intron |
| TACTGAAATGCTAGTTTCTT+GGG | 0.320010 | 8.4:+72192171 | None:intergenic |
| ATACTGAAATGCTAGTTTCT+TGG | 0.339951 | 8.4:+72192170 | None:intergenic |
| TTGCAATATTACGCTGTGTA+TGG | 0.353510 | 8.4:+72191930 | None:intergenic |
| CGTAATGTGCAGGAAGTTCT+TGG | 0.389615 | 8.4:-72191866 | MS.gene25083:CDS |
| ATGAATTTGAGCGTGTTAAT+GGG | 0.419694 | 8.4:-72192131 | MS.gene25083:CDS |
| GCACATTACGAGTCATAATC+TGG | 0.434087 | 8.4:+72191879 | None:intergenic |
| TTCAGTATCTTGAAGAGCTC+AGG | 0.435994 | 8.4:-72192155 | MS.gene25083:CDS |
| CCAAACTTAATGAAGGCATA+AGG | 0.453452 | 8.4:+72192084 | None:intergenic |
| TACGCTGTGTATGGGTATCC+TGG | 0.475049 | 8.4:+72191939 | None:intergenic |
| TGCAATATTACGCTGTGTAT+GGG | 0.492545 | 8.4:+72191931 | None:intergenic |
| CTTAATGAAGGCATAAGGTC+TGG | 0.498141 | 8.4:+72192089 | None:intergenic |
| TTACTTGACAATGTGCGATC+GGG | 0.504698 | 8.4:-72192199 | MS.gene25083:CDS |
| TGTTACTGATGGTCTTCCAT+TGG | 0.518821 | 8.4:-72193072 | MS.gene25083:CDS |
| ATATTTACCAAACTTAATGA+AGG | 0.520074 | 8.4:+72192077 | None:intergenic |
| TCTTGGTGTTGGTGAACAGT+TGG | 0.534835 | 8.4:-72191849 | MS.gene25083:CDS |
| TCTTCCATTGGCTGAAGGAA+TGG | 0.536609 | 8.4:-72193060 | MS.gene25083:CDS |
| TCATCCATTCCTTCAGCCAA+TGG | 0.552384 | 8.4:+72193056 | None:intergenic |
| AAGTTATATTATAGAAGGAC+GGG | 0.553827 | 8.4:-72192226 | MS.gene25083:intron |
| GATTATGACTCGTAATGTGC+AGG | 0.554451 | 8.4:-72191876 | MS.gene25083:CDS |
| GTTACTTGACAATGTGCGAT+CGG | 0.557699 | 8.4:-72192200 | MS.gene25083:CDS |
| CAAGTTATATTATAGAAGGA+CGG | 0.558674 | 8.4:-72192227 | MS.gene25083:intron |
| GTGCAGGAAGTTCTTGGTGT+TGG | 0.566733 | 8.4:-72191860 | MS.gene25083:CDS |
| GAGGGCATAATGAGGCATCA+AGG | 0.574125 | 8.4:-72192965 | MS.gene25083:CDS |
| GATGGTCTTCCATTGGCTGA+AGG | 0.584000 | 8.4:-72193065 | MS.gene25083:CDS |
| ATGATTGCCCGTGTTACTGA+TGG | 0.584813 | 8.4:-72193083 | MS.gene25083:CDS |
| TCAAGGATGTCAGTTGAAAG+TGG | 0.590185 | 8.4:-72192948 | MS.gene25083:intron |
| GAAGACAAAGAAACTTTACC+AGG | 0.594068 | 8.4:-72191957 | MS.gene25083:CDS |
| ACTGAAATGCTAGTTTCTTG+GGG | 0.603059 | 8.4:+72192172 | None:intergenic |
| TGTTTAAGAATCTATCAAGA+GGG | 0.608370 | 8.4:-72192983 | MS.gene25083:CDS |
| TTGTTTAAGAATCTATCAAG+AGG | 0.618641 | 8.4:-72192984 | MS.gene25083:CDS |
| TCTATCAAGAGGGCATAATG+AGG | 0.621786 | 8.4:-72192973 | MS.gene25083:CDS |
| TGGAAGACCATCAGTAACAC+GGG | 0.633889 | 8.4:+72193076 | None:intergenic |
| ATGGAAGACCATCAGTAACA+CGG | 0.637792 | 8.4:+72193075 | None:intergenic |
| GATGCTCGTGATCTTCAAGA+TGG | 0.664408 | 8.4:-72193035 | MS.gene25083:CDS |
| CATTACGAGTCATAATCTGG+TGG | 0.675355 | 8.4:+72191882 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTATTAAACAAAAGAAAAA+AGG | + | chr8.4:72192871-72192890 | None:intergenic | 15.0% |
| !! | ATATTTACCAAACTTAATGA+AGG | + | chr8.4:72192786-72192805 | None:intergenic | 20.0% |
| !! | CAAACAAGTTATATTATAGA+AGG | - | chr8.4:72192629-72192648 | MS.gene25083:intron | 20.0% |
| !! | TATAAGCATAATATATGAGA+AGG | + | chr8.4:72191957-72191976 | None:intergenic | 20.0% |
| !!! | AATATAACTTGTTTGTGTTA+AGG | + | chr8.4:72192624-72192643 | None:intergenic | 20.0% |
| !!! | TAAGTTATTCTCTGTATTTT+CGG | - | chr8.4:72193038-72193057 | MS.gene25083:CDS | 20.0% |
| !!! | TTCATTATCTTGAAATTTTG+TGG | - | chr8.4:72192525-72192544 | MS.gene25083:intron | 20.0% |
| ! | AAAGGTAAATTAAACACCAT+TGG | + | chr8.4:72192853-72192872 | None:intergenic | 25.0% |
| ! | AAGTTATATTATAGAAGGAC+GGG | - | chr8.4:72192634-72192653 | MS.gene25083:intron | 25.0% |
| ! | CAAGTTATATTATAGAAGGA+CGG | - | chr8.4:72192633-72192652 | MS.gene25083:intron | 25.0% |
| ! | CTGTTGAATCACAATTATAT+TGG | - | chr8.4:72192417-72192436 | MS.gene25083:intron | 25.0% |
| ! | TCAATCAAAACAAAGAATAC+AGG | + | chr8.4:72192385-72192404 | None:intergenic | 25.0% |
| ! | TGTTTAAGAATCTATCAAGA+GGG | - | chr8.4:72191877-72191896 | MS.gene25083:CDS | 25.0% |
| ! | TTGTTTAAGAATCTATCAAG+AGG | - | chr8.4:72191876-72191895 | MS.gene25083:CDS | 25.0% |
| !! | AAAACAATATCAACTTGCTT+GGG | + | chr8.4:72192507-72192526 | None:intergenic | 25.0% |
| !! | TCCTTATGTTTTCAATAATG+AGG | - | chr8.4:72192012-72192031 | MS.gene25083:intron | 25.0% |
| !!! | TATATTGGTGCTTTTGTATA+TGG | - | chr8.4:72192432-72192451 | MS.gene25083:intron | 25.0% |
| AATGAATTTGAGCGTGTTAA+TGG | - | chr8.4:72192728-72192747 | MS.gene25083:intron | 30.0% | |
| AATTGTGATTCAACAGAGAA+AGG | + | chr8.4:72192413-72192432 | None:intergenic | 30.0% | |
| ATAGTATAACAGCAGAACAA+TGG | + | chr8.4:72192077-72192096 | None:intergenic | 30.0% | |
| ATGAATTTGAGCGTGTTAAT+GGG | - | chr8.4:72192729-72192748 | MS.gene25083:intron | 30.0% | |
| GAAGGATACTGAAAAACATA+AGG | + | chr8.4:72191939-72191958 | None:intergenic | 30.0% | |
| GCCTCATTATTGAAAACATA+AGG | + | chr8.4:72192016-72192035 | None:intergenic | 30.0% | |
| TAGTATAACAGCAGAACAAT+GGG | + | chr8.4:72192076-72192095 | None:intergenic | 30.0% | |
| TTATTGAAAACATAAGGACC+TGG | + | chr8.4:72192010-72192029 | None:intergenic | 30.0% | |
| ! | ATACTGAAATGCTAGTTTCT+TGG | + | chr8.4:72192693-72192712 | None:intergenic | 30.0% |
| ! | GAAAACAATATCAACTTGCT+TGG | + | chr8.4:72192508-72192527 | None:intergenic | 30.0% |
| ! | TACTGAAATGCTAGTTTCTT+GGG | + | chr8.4:72192692-72192711 | None:intergenic | 30.0% |
| ! | TGTTTGTGTTAAGGTCATTT+TGG | + | chr8.4:72192615-72192634 | None:intergenic | 30.0% |
| !! | AAAAAGAGAAGAGTGCTAAA+TGG | + | chr8.4:72192830-72192849 | None:intergenic | 30.0% |
| !! | GTAGTGTTCTATTTTTCACA+TGG | + | chr8.4:72192461-72192480 | None:intergenic | 30.0% |
| !! | GTTTGTGTTAAGGTCATTTT+GGG | + | chr8.4:72192614-72192633 | None:intergenic | 30.0% |
| AGTGCAGCAATTATAATGAG+TGG | - | chr8.4:72192116-72192135 | MS.gene25083:CDS | 35.0% | |
| ATCAGATTGAAATGCTTGAC+TGG | - | chr8.4:72192277-72192296 | MS.gene25083:intron | 35.0% | |
| CCTTATGCCTTCATTAAGTT+TGG | - | chr8.4:72192776-72192795 | MS.gene25083:intron | 35.0% | |
| GAAGACAAAGAAACTTTACC+AGG | - | chr8.4:72192903-72192922 | MS.gene25083:intron | 35.0% | |
| GTGCAGCAATTATAATGAGT+GGG | - | chr8.4:72192117-72192136 | MS.gene25083:CDS | 35.0% | |
| TCAGATTGAAATGCTTGACT+GGG | - | chr8.4:72192278-72192297 | MS.gene25083:intron | 35.0% | |
| TGCAATATTACGCTGTGTAT+GGG | + | chr8.4:72192932-72192951 | None:intergenic | 35.0% | |
| TGTTATCTTCCACTAAATGC+CGG | - | chr8.4:72192252-72192271 | MS.gene25083:intron | 35.0% | |
| TTATACTATGAGACACATCC+AGG | - | chr8.4:72191989-72192008 | MS.gene25083:intron | 35.0% | |
| TTGAACATTAGAGGAAGCTA+TGG | - | chr8.4:72192359-72192378 | MS.gene25083:intron | 35.0% | |
| TTGCAATATTACGCTGTGTA+TGG | + | chr8.4:72192933-72192952 | None:intergenic | 35.0% | |
| ! | ACTGAAATGCTAGTTTCTTG+GGG | + | chr8.4:72192691-72192710 | None:intergenic | 35.0% |
| ! | CATATGCAGTATTGAACGAT+AGG | - | chr8.4:72192171-72192190 | MS.gene25083:CDS | 35.0% |
| ! | CCAAACTTAATGAAGGCATA+AGG | + | chr8.4:72192779-72192798 | None:intergenic | 35.0% |
| ! | TCTACAGTCTGTTGTTACTT+TGG | - | chr8.4:72192225-72192244 | MS.gene25083:intron | 35.0% |
| !! | ATTGCTAGTTCTGCAAATCT+TGG | - | chr8.4:72192332-72192351 | MS.gene25083:intron | 35.0% |
| !! | CTACAGTCTGTTGTTACTTT+GGG | - | chr8.4:72192226-72192245 | MS.gene25083:intron | 35.0% |
| !!! | CTTGGTGTTTTGAACATTAG+AGG | - | chr8.4:72192350-72192369 | MS.gene25083:intron | 35.0% |
| AAGCATTTCAATCTGATGCC+CGG | + | chr8.4:72192274-72192293 | None:intergenic | 40.0% | |
| ATGGAAGACCATCAGTAACA+CGG | + | chr8.4:72191788-72191807 | None:intergenic | 40.0% | |
| CATTACGAGTCATAATCTGG+TGG | + | chr8.4:72192981-72193000 | None:intergenic | 40.0% | |
| GATTATGACTCGTAATGTGC+AGG | - | chr8.4:72192984-72193003 | MS.gene25083:CDS | 40.0% | |
| GCACATTACGAGTCATAATC+TGG | + | chr8.4:72192984-72193003 | None:intergenic | 40.0% | |
| GTTACTTGACAATGTGCGAT+CGG | - | chr8.4:72192660-72192679 | MS.gene25083:intron | 40.0% | |
| GTTATCTTCCACTAAATGCC+GGG | - | chr8.4:72192253-72192272 | MS.gene25083:intron | 40.0% | |
| TCAAGGATGTCAGTTGAAAG+TGG | - | chr8.4:72191912-72191931 | MS.gene25083:CDS | 40.0% | |
| TGTTACTGATGGTCTTCCAT+TGG | - | chr8.4:72191788-72191807 | MS.gene25083:intron | 40.0% | |
| TTACTTGACAATGTGCGATC+GGG | - | chr8.4:72192661-72192680 | MS.gene25083:intron | 40.0% | |
| TTCAGTATCTTGAAGAGCTC+AGG | - | chr8.4:72192705-72192724 | MS.gene25083:intron | 40.0% | |
| ! | CTTAATGAAGGCATAAGGTC+TGG | + | chr8.4:72192774-72192793 | None:intergenic | 40.0% |
| ! | GCACTCTTCTCTTTTTCCAA+TGG | - | chr8.4:72192834-72192853 | MS.gene25083:intron | 40.0% |
| ! | TCTATCAAGAGGGCATAATG+AGG | - | chr8.4:72191887-72191906 | MS.gene25083:CDS | 40.0% |
| AATTGCTGCACTCAGACACT+GGG | + | chr8.4:72192108-72192127 | None:intergenic | 45.0% | |
| CGTAATGTGCAGGAAGTTCT+TGG | - | chr8.4:72192994-72193013 | MS.gene25083:CDS | 45.0% | |
| GATGCTCGTGATCTTCAAGA+TGG | - | chr8.4:72191825-72191844 | MS.gene25083:intron | 45.0% | |
| TAATTGCTGCACTCAGACAC+TGG | + | chr8.4:72192109-72192128 | None:intergenic | 45.0% | |
| TCATCCATTCCTTCAGCCAA+TGG | + | chr8.4:72191807-72191826 | None:intergenic | 45.0% | |
| TGGAAGACCATCAGTAACAC+GGG | + | chr8.4:72191787-72191806 | None:intergenic | 45.0% | |
| ! | ATCAACTTGCTTGGGAAGCT+AGG | + | chr8.4:72192499-72192518 | None:intergenic | 45.0% |
| ! | ATGATTGCCCGTGTTACTGA+TGG | - | chr8.4:72191777-72191796 | MS.gene25083:intron | 45.0% |
| ! | TCTTCCATTGGCTGAAGGAA+TGG | - | chr8.4:72191800-72191819 | MS.gene25083:intron | 45.0% |
| !! | TCTTGGTGTTGGTGAACAGT+TGG | - | chr8.4:72193011-72193030 | MS.gene25083:CDS | 45.0% |
| ATCTGATGCCCGGCATTTAG+TGG | + | chr8.4:72192264-72192283 | None:intergenic | 50.0% | |
| TACGCTGTGTATGGGTATCC+TGG | + | chr8.4:72192924-72192943 | None:intergenic | 50.0% | |
| ! | GAGGGCATAATGAGGCATCA+AGG | - | chr8.4:72191895-72191914 | MS.gene25083:CDS | 50.0% |
| ! | GATGGTCTTCCATTGGCTGA+AGG | - | chr8.4:72191795-72191814 | MS.gene25083:intron | 50.0% |
| ! | GTGCAGGAAGTTCTTGGTGT+TGG | - | chr8.4:72193000-72193019 | MS.gene25083:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 72191762 | 72193120 | 72191762 | ID=MS.gene25083 |
| chr8.4 | mRNA | 72191762 | 72193120 | 72191762 | ID=MS.gene25083.t1;Parent=MS.gene25083 |
| chr8.4 | exon | 72192949 | 72193120 | 72192949 | ID=MS.gene25083.t1.exon1;Parent=MS.gene25083.t1 |
| chr8.4 | CDS | 72192949 | 72193120 | 72192949 | ID=cds.MS.gene25083.t1;Parent=MS.gene25083.t1 |
| chr8.4 | exon | 72192085 | 72192234 | 72192085 | ID=MS.gene25083.t1.exon2;Parent=MS.gene25083.t1 |
| chr8.4 | CDS | 72192085 | 72192234 | 72192085 | ID=cds.MS.gene25083.t1;Parent=MS.gene25083.t1 |
| chr8.4 | exon | 72191846 | 72191992 | 72191846 | ID=MS.gene25083.t1.exon3;Parent=MS.gene25083.t1 |
| chr8.4 | CDS | 72191846 | 72191992 | 72191846 | ID=cds.MS.gene25083.t1;Parent=MS.gene25083.t1 |
| chr8.4 | exon | 72191762 | 72191766 | 72191762 | ID=MS.gene25083.t1.exon4;Parent=MS.gene25083.t1 |
| chr8.4 | CDS | 72191762 | 72191766 | 72191762 | ID=cds.MS.gene25083.t1;Parent=MS.gene25083.t1 |
| Gene Sequence |
| Protein sequence |
