AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052242


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052242.t1 MTR_3g087830 97.368 114 3 0 1 114 607 720 7.37e-69 219
MS.gene052242.t1 MTR_8g091530 74.138 58 15 0 57 114 704 761 1.03e-21 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052242.t1 AT4G39680 83.333 36 6 0 75 110 597 632 5.41e-14 67.0
MS.gene052242.t1 AT4G39680 83.333 36 6 0 75 110 597 632 5.41e-14 67.0

Find 52 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTACCAATGGTGGCGGAGG+TGG 0.329485 3.1:-70682530 None:intergenic
TGCTGCGAAACTTGCGGCTC+AGG 0.367848 3.1:+70682700 MS.gene052242:CDS
GGTGGTGCGGGAGGAACTAT+TGG 0.370795 3.1:-70682446 None:intergenic
GGCGGAGGTGGGAGAGTGAC+TGG 0.377606 3.1:-70682518 None:intergenic
GGCTGTGAAGAGGGTGGTGC+GGG 0.392874 3.1:-70682458 None:intergenic
GGCCTTACCAATGGTGGCGG+AGG 0.397144 3.1:-70682533 None:intergenic
TGCTGACGAGGGGAAGCCTC+TGG 0.436452 3.1:-70682479 None:intergenic
ATAGGTGGGTCCACTTTCTC+AGG 0.440175 3.1:-70682599 None:intergenic
GAAGCCTCTGGCTGTGAAGA+GGG 0.449251 3.1:-70682467 None:intergenic
ACCACCCCTTCCTGAGAAAG+TGG 0.451879 3.1:+70682589 MS.gene052242:CDS
TCAGGAAGGGGTGGTGGCGA+TGG 0.460747 3.1:-70682581 None:intergenic
GGCGTTCCCTAGCAACTGGT+GGG 0.468982 3.1:-70682556 None:intergenic
TGGGTCCACTTTCTCAGGAA+GGG 0.472429 3.1:-70682594 None:intergenic
AGGCTTCCCCTCGTCAGCAC+AGG 0.479056 3.1:+70682483 MS.gene052242:CDS
GAAGGGGTGGTGGCGATGGA+AGG 0.486557 3.1:-70682577 None:intergenic
CACCTCCGCCACCATTGGTA+AGG 0.487137 3.1:+70682531 MS.gene052242:CDS
GGAAGGCGTTCCCTAGCAAC+TGG 0.491793 3.1:-70682560 None:intergenic
CGTTCCCTAGCAACTGGTGG+GGG 0.497494 3.1:-70682554 None:intergenic
AGATCATCCAGAGTGACAAT+AGG 0.498410 3.1:-70682617 None:intergenic
TTACCAATGGTGGCGGAGGT+GGG 0.506883 3.1:-70682529 None:intergenic
AGGCGTTCCCTAGCAACTGG+TGG 0.509948 3.1:-70682557 None:intergenic
ACTTTCTCAGGAAGGGGTGG+TGG 0.513958 3.1:-70682587 None:intergenic
TGTGAAGAGGGTGGTGCGGG+AGG 0.521080 3.1:-70682455 None:intergenic
GGGTCCACTTTCTCAGGAAG+GGG 0.524988 3.1:-70682593 None:intergenic
TCTCCCACCTCCGCCACCAT+TGG 0.525575 3.1:+70682526 MS.gene052242:CDS
TCCACTTTCTCAGGAAGGGG+TGG 0.525720 3.1:-70682590 None:intergenic
TGGCTGTGAAGAGGGTGGTG+CGG 0.535809 3.1:-70682459 None:intergenic
GTGGGTCCACTTTCTCAGGA+AGG 0.554750 3.1:-70682595 None:intergenic
GGACCCACCTATTGTCACTC+TGG 0.556935 3.1:+70682610 MS.gene052242:CDS
GGCTTCCCCTCGTCAGCACA+GGG 0.565114 3.1:+70682484 MS.gene052242:CDS
GCCTCTGGCTGTGAAGAGGG+TGG 0.565638 3.1:-70682464 None:intergenic
TCATCCAGAGTGACAATAGG+TGG 0.568024 3.1:-70682614 None:intergenic
TGTTGCTCCCTGTGCTGACG+AGG 0.570630 3.1:-70682491 None:intergenic
GCTGCGAAACTTGCGGCTCA+GGG 0.574547 3.1:+70682701 MS.gene052242:CDS
ACCACCCTCTTCACAGCCAG+AGG 0.575603 3.1:+70682463 MS.gene052242:CDS
GGGTAAGAGTACGAGGCAGT+AGG 0.576110 3.1:+70682721 MS.gene052242:CDS
GGAAGCCTCTGGCTGTGAAG+AGG 0.578072 3.1:-70682468 None:intergenic
GCGTTCCCTAGCAACTGGTG+GGG 0.589857 3.1:-70682555 None:intergenic
ATTGGAGCACTGTTGACAGC+AGG 0.595299 3.1:-70682428 None:intergenic
TTGCTCCCTGTGCTGACGAG+GGG 0.600190 3.1:-70682489 None:intergenic
GTAAAACCACTGCAACTCCT+CGG 0.602190 3.1:+70682645 MS.gene052242:CDS
GCACTGTTGACAGCAGGAGT+AGG 0.605864 3.1:-70682422 None:intergenic
GTTGACAGCAGGAGTAGGAG+TGG 0.608389 3.1:-70682417 None:intergenic
ACAGCAGGAGTAGGAGTGGG+AGG 0.608778 3.1:-70682413 None:intergenic
CGGCTCAGGGTAAGAGTACG+AGG 0.610619 3.1:+70682714 MS.gene052242:CDS
TTGACAGCAGGAGTAGGAGT+GGG 0.613695 3.1:-70682416 None:intergenic
GTTGCTCCCTGTGCTGACGA+GGG 0.624628 3.1:-70682490 None:intergenic
GCAAGTTGCTGCGAAACTTG+CGG 0.641051 3.1:+70682694 MS.gene052242:CDS
GCAACTTGCTCTTCTGACAA+AGG 0.648763 3.1:-70682680 None:intergenic
AAAGGTAAATAATAGATCCG+AGG 0.649093 3.1:-70682662 None:intergenic
CATCCAGAGTGACAATAGGT+GGG 0.665205 3.1:-70682613 None:intergenic
ATAGATCCGAGGAGTTGCAG+TGG 0.681963 3.1:-70682651 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAGGTAAATAATAGATCCG+AGG - chr3.1:70682665-70682684 None:intergenic 30.0%
AGATCATCCAGAGTGACAAT+AGG - chr3.1:70682620-70682639 None:intergenic 40.0%
CATCCAGAGTGACAATAGGT+GGG - chr3.1:70682616-70682635 None:intergenic 45.0%
GTAAAACCACTGCAACTCCT+CGG + chr3.1:70682645-70682664 MS.gene052242:CDS 45.0%
TCATCCAGAGTGACAATAGG+TGG - chr3.1:70682617-70682636 None:intergenic 45.0%
! GCAACTTGCTCTTCTGACAA+AGG - chr3.1:70682683-70682702 None:intergenic 45.0%
!! GAGGAGTTGCAGTGGTTTTA+CGG - chr3.1:70682646-70682665 None:intergenic 45.0%
ATAGATCCGAGGAGTTGCAG+TGG - chr3.1:70682654-70682673 None:intergenic 50.0%
ATAGGTGGGTCCACTTTCTC+AGG - chr3.1:70682602-70682621 None:intergenic 50.0%
GCAAGTTGCTGCGAAACTTG+CGG + chr3.1:70682694-70682713 MS.gene052242:CDS 50.0%
TGGGTCCACTTTCTCAGGAA+GGG - chr3.1:70682597-70682616 None:intergenic 50.0%
TTGACAGCAGGAGTAGGAGT+GGG - chr3.1:70682419-70682438 None:intergenic 50.0%
!! ATTGGAGCACTGTTGACAGC+AGG - chr3.1:70682431-70682450 None:intergenic 50.0%
ACCACCCCTTCCTGAGAAAG+TGG + chr3.1:70682589-70682608 MS.gene052242:CDS 55.0%
ACTTTCTCAGGAAGGGGTGG+TGG - chr3.1:70682590-70682609 None:intergenic 55.0%
GAAGCCTCTGGCTGTGAAGA+GGG - chr3.1:70682470-70682489 None:intergenic 55.0%
GCACTGTTGACAGCAGGAGT+AGG - chr3.1:70682425-70682444 None:intergenic 55.0%
GGACCCACCTATTGTCACTC+TGG + chr3.1:70682610-70682629 MS.gene052242:CDS 55.0%
GGGTCCACTTTCTCAGGAAG+GGG - chr3.1:70682596-70682615 None:intergenic 55.0%
GTGGGTCCACTTTCTCAGGA+AGG - chr3.1:70682598-70682617 None:intergenic 55.0%
GTTGACAGCAGGAGTAGGAG+TGG - chr3.1:70682420-70682439 None:intergenic 55.0%
TCCACTTTCTCAGGAAGGGG+TGG - chr3.1:70682593-70682612 None:intergenic 55.0%
!! TTACCAATGGTGGCGGAGGT+GGG - chr3.1:70682532-70682551 None:intergenic 55.0%
AAGGCCCCCACCAGTTGCTA+GGG + chr3.1:70682550-70682569 MS.gene052242:CDS 60.0%
ACAGCAGGAGTAGGAGTGGG+AGG - chr3.1:70682416-70682435 None:intergenic 60.0%
ACCACCCTCTTCACAGCCAG+AGG + chr3.1:70682463-70682482 MS.gene052242:CDS 60.0%
ACTGGTGGGGGCCTTACCAA+TGG - chr3.1:70682545-70682564 None:intergenic 60.0%
AGGCGTTCCCTAGCAACTGG+TGG - chr3.1:70682560-70682579 None:intergenic 60.0%
CACCTCCGCCACCATTGGTA+AGG + chr3.1:70682531-70682550 MS.gene052242:CDS 60.0%
CGTTCCCTAGCAACTGGTGG+GGG - chr3.1:70682557-70682576 None:intergenic 60.0%
GCGTTCCCTAGCAACTGGTG+GGG - chr3.1:70682558-70682577 None:intergenic 60.0%
GCTGCGAAACTTGCGGCTCA+GGG + chr3.1:70682701-70682720 MS.gene052242:CDS 60.0%
GGAAGCCTCTGGCTGTGAAG+AGG - chr3.1:70682471-70682490 None:intergenic 60.0%
GGCGTTCCCTAGCAACTGGT+GGG - chr3.1:70682559-70682578 None:intergenic 60.0%
GGTGGTGCGGGAGGAACTAT+TGG - chr3.1:70682449-70682468 None:intergenic 60.0%
TAAGGCCCCCACCAGTTGCT+AGG + chr3.1:70682549-70682568 MS.gene052242:CDS 60.0%
TGCTGCGAAACTTGCGGCTC+AGG + chr3.1:70682700-70682719 MS.gene052242:CDS 60.0%
TGGCTGTGAAGAGGGTGGTG+CGG - chr3.1:70682462-70682481 None:intergenic 60.0%
! CGGCTCAGGGTAAGAGTACG+AGG + chr3.1:70682714-70682733 MS.gene052242:CDS 60.0%
! GTTGCTCCCTGTGCTGACGA+GGG - chr3.1:70682493-70682512 None:intergenic 60.0%
! TGTTGCTCCCTGTGCTGACG+AGG - chr3.1:70682494-70682513 None:intergenic 60.0%
! TTGCTCCCTGTGCTGACGAG+GGG - chr3.1:70682492-70682511 None:intergenic 60.0%
!! CTTACCAATGGTGGCGGAGG+TGG - chr3.1:70682533-70682552 None:intergenic 60.0%
!! GGAAGGCGTTCCCTAGCAAC+TGG - chr3.1:70682563-70682582 None:intergenic 60.0%
AGGCTTCCCCTCGTCAGCAC+AGG + chr3.1:70682483-70682502 MS.gene052242:CDS 65.0%
GCCTCTGGCTGTGAAGAGGG+TGG - chr3.1:70682467-70682486 None:intergenic 65.0%
GGCTGTGAAGAGGGTGGTGC+GGG - chr3.1:70682461-70682480 None:intergenic 65.0%
GGCTTCCCCTCGTCAGCACA+GGG + chr3.1:70682484-70682503 MS.gene052242:CDS 65.0%
GGGGGCCTTACCAATGGTGG+CGG - chr3.1:70682539-70682558 None:intergenic 65.0%
GGTGGGGGCCTTACCAATGG+TGG - chr3.1:70682542-70682561 None:intergenic 65.0%
TCTCCCACCTCCGCCACCAT+TGG + chr3.1:70682526-70682545 MS.gene052242:CDS 65.0%
TGCTGACGAGGGGAAGCCTC+TGG - chr3.1:70682482-70682501 None:intergenic 65.0%
TGTGAAGAGGGTGGTGCGGG+AGG - chr3.1:70682458-70682477 None:intergenic 65.0%
! GAAGGGGTGGTGGCGATGGA+AGG - chr3.1:70682580-70682599 None:intergenic 65.0%
! TCAGGAAGGGGTGGTGGCGA+TGG - chr3.1:70682584-70682603 None:intergenic 65.0%
!! GGCCTTACCAATGGTGGCGG+AGG - chr3.1:70682536-70682555 None:intergenic 65.0%
GGCGGAGGTGGGAGAGTGAC+TGG - chr3.1:70682521-70682540 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 70682398 70682742 70682398 ID=MS.gene052242
chr3.1 mRNA 70682398 70682742 70682398 ID=MS.gene052242.t1;Parent=MS.gene052242
chr3.1 exon 70682398 70682742 70682398 ID=MS.gene052242.t1.exon1;Parent=MS.gene052242.t1
chr3.1 CDS 70682398 70682742 70682398 ID=cds.MS.gene052242.t1;Parent=MS.gene052242.t1
Gene Sequence

>MS.gene052242

ATGAAGTTAGAAGCTCCTCCCACTCCTACTCCTGCTGTCAACAGTGCTCCAATAGTTCCTCCCGCACCACCCTCTTCACAGCCAGAGGCTTCCCCTCGTCAGCACAGGGAGCAACATCTTCCAGTCACTCTCCCACCTCCGCCACCATTGGTAAGGCCCCCACCAGTTGCTAGGGAACGCCTTCCATCGCCACCACCCCTTCCTGAGAAAGTGGACCCACCTATTGTCACTCTGGATGATCTCTTCCGTAAAACCACTGCAACTCCTCGGATCTATTATTTACCTTTGTCAGAAGAGCAAGTTGCTGCGAAACTTGCGGCTCAGGGTAAGAGTACGAGGCAGTAG

Protein sequence

>MS.gene052242.t1

MKLEAPPTPTPAVNSAPIVPPAPPSSQPEASPRQHREQHLPVTLPPPPPLVRPPPVARERLPSPPPLPEKVDPPIVTLDDLFRKTTATPRIYYLPLSEEQVAAKLAAQGKSTRQ