Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051831.t1 | XP_013458443.1 | 95 | 80 | 4 | 0 | 75 | 154 | 4 | 83 | 1.10E-37 | 166.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051831.t1 | I3SLJ0 | 95.0 | 80 | 4 | 0 | 75 | 154 | 4 | 83 | 7.6e-38 | 166.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050359 | MS.gene051831 | 0.807863 | 4.11E-50 | -1.69E-46 |
| MS.gene051184 | MS.gene051831 | 0.832574 | 8.75E-56 | -1.69E-46 |
| MS.gene051831 | MS.gene053726 | 0.800671 | 1.29E-48 | -1.69E-46 |
| MS.gene051831 | MS.gene05439 | 0.803315 | 3.70E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene055442 | 0.801457 | 8.92E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene056699 | 0.800128 | 1.67E-48 | -1.69E-46 |
| MS.gene051831 | MS.gene056787 | 0.815251 | 1.02E-51 | -1.69E-46 |
| MS.gene051831 | MS.gene056849 | 0.807146 | 5.83E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene058278 | 0.801621 | 8.26E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene058429 | 0.807421 | 5.10E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene058758 | 0.80712 | 5.90E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene059050 | 0.801299 | 9.61E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene06010 | 0.808773 | 2.63E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene060932 | 0.807372 | 5.22E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene061958 | 0.836919 | 7.06E-57 | -1.69E-46 |
| MS.gene051831 | MS.gene065504 | 0.804239 | 2.38E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene066427 | 0.800123 | 1.67E-48 | -1.69E-46 |
| MS.gene051831 | MS.gene066513 | 0.806185 | 9.30E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene067043 | 0.84186 | 3.68E-58 | -1.69E-46 |
| MS.gene051831 | MS.gene070671 | 0.838816 | 2.30E-57 | -1.69E-46 |
| MS.gene051831 | MS.gene072092 | -0.800288 | 1.55E-48 | -1.69E-46 |
| MS.gene051831 | MS.gene072709 | 0.803877 | 2.83E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene074100 | 0.817086 | 3.96E-52 | -1.69E-46 |
| MS.gene051831 | MS.gene20392 | 0.826439 | 2.71E-54 | -1.69E-46 |
| MS.gene051831 | MS.gene20608 | 0.810482 | 1.13E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene21282 | 0.80272 | 4.91E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene23464 | 0.835643 | 1.49E-56 | -1.69E-46 |
| MS.gene051831 | MS.gene23484 | 0.801041 | 1.09E-48 | -1.69E-46 |
| MS.gene051831 | MS.gene25083 | 0.800144 | 1.65E-48 | -1.69E-46 |
| MS.gene051831 | MS.gene25688 | 0.871135 | 8.61E-67 | -1.69E-46 |
| MS.gene051831 | MS.gene27997 | 0.802082 | 6.64E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene28491 | 0.823989 | 1.03E-53 | -1.69E-46 |
| MS.gene051831 | MS.gene28798 | 0.807528 | 4.84E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene29047 | 0.819278 | 1.26E-52 | -1.69E-46 |
| MS.gene051831 | MS.gene29109 | 0.803515 | 3.36E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene30808 | 0.830059 | 3.63E-55 | -1.69E-46 |
| MS.gene051831 | MS.gene31862 | 0.812489 | 4.13E-51 | -1.69E-46 |
| MS.gene051831 | MS.gene32801 | 0.804941 | 1.70E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene32863 | 0.812937 | 3.30E-51 | -1.69E-46 |
| MS.gene051831 | MS.gene33056 | 0.803394 | 3.56E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene33177 | 0.801033 | 1.09E-48 | -1.69E-46 |
| MS.gene051831 | MS.gene33180 | 0.812714 | 3.69E-51 | -1.69E-46 |
| MS.gene051831 | MS.gene34340 | 0.800665 | 1.30E-48 | -1.69E-46 |
| MS.gene051831 | MS.gene34444 | 0.803474 | 3.43E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene34691 | 0.803032 | 4.23E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene35934 | 0.813684 | 2.26E-51 | -1.69E-46 |
| MS.gene051831 | MS.gene37468 | 0.808754 | 2.65E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene37988 | 0.805476 | 1.31E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene37989 | 0.809282 | 2.04E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene40006 | 0.807252 | 5.53E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene40608 | 0.800819 | 1.21E-48 | -1.69E-46 |
| MS.gene051831 | MS.gene40991 | 0.806532 | 7.86E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene42128 | 0.818898 | 1.54E-52 | -1.69E-46 |
| MS.gene051831 | MS.gene42295 | 0.832625 | 8.50E-56 | -1.69E-46 |
| MS.gene051831 | MS.gene43289 | 0.807037 | 6.15E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene45374 | 0.836498 | 9.03E-57 | -1.69E-46 |
| MS.gene051831 | MS.gene47674 | 0.803078 | 4.14E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene47695 | 0.805221 | 1.48E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene47995 | 0.809348 | 1.98E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene48283 | 0.838074 | 3.57E-57 | -1.69E-46 |
| MS.gene051831 | MS.gene48393 | -0.80778 | 4.28E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene49733 | 0.820511 | 6.60E-53 | -1.69E-46 |
| MS.gene051831 | MS.gene49816 | 0.80207 | 6.68E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene51557 | 0.833918 | 4.05E-56 | -1.69E-46 |
| MS.gene051831 | MS.gene53070 | 0.802947 | 4.40E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene54377 | 0.8083 | 3.31E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene54380 | 0.804776 | 1.84E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene56039 | 0.811814 | 5.80E-51 | -1.69E-46 |
| MS.gene051831 | MS.gene57028 | 0.81271 | 3.70E-51 | -1.69E-46 |
| MS.gene051831 | MS.gene57128 | 0.803031 | 4.23E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene57298 | 0.807889 | 4.05E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene58134 | 0.80125 | 9.84E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene58467 | 0.80056 | 1.36E-48 | -1.69E-46 |
| MS.gene051831 | MS.gene58616 | 0.816413 | 5.60E-52 | -1.69E-46 |
| MS.gene051831 | MS.gene59086 | 0.803983 | 2.69E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene60144 | 0.800082 | 1.70E-48 | -1.69E-46 |
| MS.gene051831 | MS.gene60302 | 0.813652 | 2.30E-51 | -1.69E-46 |
| MS.gene051831 | MS.gene60343 | 0.807969 | 3.90E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene60374 | 0.822983 | 1.77E-53 | -1.69E-46 |
| MS.gene051831 | MS.gene61064 | 0.838999 | 2.06E-57 | -1.69E-46 |
| MS.gene051831 | MS.gene62461 | 0.802668 | 5.03E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene62501 | 0.81543 | 9.28E-52 | -1.69E-46 |
| MS.gene051831 | MS.gene62676 | 0.802404 | 5.70E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene62895 | 0.8187 | 1.71E-52 | -1.69E-46 |
| MS.gene051831 | MS.gene64284 | 0.807872 | 4.09E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene64665 | 0.810384 | 1.18E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene67412 | 0.808435 | 3.10E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene69442 | 0.804958 | 1.68E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene69691 | 0.846653 | 1.90E-59 | -1.69E-46 |
| MS.gene051831 | MS.gene69692 | 0.834116 | 3.61E-56 | -1.69E-46 |
| MS.gene051831 | MS.gene70154 | 0.80104 | 1.09E-48 | -1.69E-46 |
| MS.gene051831 | MS.gene71879 | 0.810468 | 1.14E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene71940 | 0.812266 | 4.62E-51 | -1.69E-46 |
| MS.gene051831 | MS.gene72312 | 0.818847 | 1.58E-52 | -1.69E-46 |
| MS.gene051831 | MS.gene72748 | 0.801634 | 8.21E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene73245 | 0.8095 | 1.84E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene74510 | 0.807521 | 4.85E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene75185 | 0.804148 | 2.48E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene75412 | 0.824653 | 7.18E-54 | -1.69E-46 |
| MS.gene051831 | MS.gene75564 | 0.807917 | 4.00E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene76635 | 0.80055 | 1.37E-48 | -1.69E-46 |
| MS.gene051831 | MS.gene77361 | 0.815274 | 1.01E-51 | -1.69E-46 |
| MS.gene051831 | MS.gene80557 | 0.807248 | 5.55E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene81715 | 0.817417 | 3.33E-52 | -1.69E-46 |
| MS.gene051831 | MS.gene82269 | 0.800044 | 1.73E-48 | -1.69E-46 |
| MS.gene051831 | MS.gene83022 | 0.835118 | 2.02E-56 | -1.69E-46 |
| MS.gene051831 | MS.gene83687 | 0.819324 | 1.23E-52 | -1.69E-46 |
| MS.gene051831 | MS.gene84498 | 0.807636 | 4.59E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene86455 | 0.801996 | 6.92E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene87403 | 0.82181 | 3.31E-53 | -1.69E-46 |
| MS.gene051831 | MS.gene88184 | 0.817952 | 2.53E-52 | -1.69E-46 |
| MS.gene051831 | MS.gene88649 | 0.811211 | 7.84E-51 | -1.69E-46 |
| MS.gene051831 | MS.gene90604 | 0.808113 | 3.63E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene90882 | 0.813124 | 3.00E-51 | -1.69E-46 |
| MS.gene051831 | MS.gene94674 | 0.801926 | 7.15E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene95332 | 0.818245 | 2.17E-52 | -1.69E-46 |
| MS.gene051831 | MS.gene95339 | 0.817176 | 3.78E-52 | -1.69E-46 |
| MS.gene051831 | MS.gene96973 | 0.817673 | 2.92E-52 | -1.69E-46 |
| MS.gene051831 | MS.gene97003 | 0.808138 | 3.59E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene97638 | 0.80685 | 6.73E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene97896 | 0.832861 | 7.42E-56 | -1.69E-46 |
| MS.gene051831 | MS.gene99525 | 0.800063 | 1.72E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene051831 | MS.gene25687 | PPI |
| MS.gene051831 | MS.gene012242 | PPI |
| MS.gene051831 | MS.gene24849 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051831.t1 | MTR_4g125770 | 93.827 | 81 | 5 | 0 | 75 | 155 | 4 | 84 | 1.00e-53 | 167 |
| MS.gene051831.t1 | MTR_4g125770 | 90.123 | 81 | 5 | 1 | 75 | 155 | 4 | 81 | 3.05e-49 | 155 |
| MS.gene051831.t1 | MTR_4g125770 | 93.333 | 45 | 3 | 0 | 111 | 155 | 3 | 47 | 7.42e-28 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051831.t1 | AT4G26060 | 50.746 | 67 | 31 | 1 | 89 | 155 | 14 | 78 | 6.40e-18 | 75.5 |
| MS.gene051831.t1 | AT5G57060 | 65.000 | 40 | 14 | 0 | 116 | 155 | 40 | 79 | 6.62e-15 | 67.8 |
| MS.gene051831.t1 | AT5G57060 | 65.000 | 40 | 14 | 0 | 116 | 155 | 40 | 79 | 6.62e-15 | 67.8 |
| MS.gene051831.t1 | AT5G57060 | 65.000 | 40 | 14 | 0 | 116 | 155 | 40 | 79 | 6.62e-15 | 67.8 |
| MS.gene051831.t1 | AT5G57060 | 65.000 | 40 | 14 | 0 | 116 | 155 | 40 | 79 | 6.62e-15 | 67.8 |
| MS.gene051831.t1 | AT5G57060 | 65.000 | 40 | 14 | 0 | 116 | 155 | 40 | 79 | 6.62e-15 | 67.8 |
| MS.gene051831.t1 | AT5G57060 | 65.000 | 40 | 14 | 0 | 116 | 155 | 13 | 52 | 7.90e-15 | 66.6 |
| MS.gene051831.t1 | AT5G57060 | 65.000 | 40 | 14 | 0 | 116 | 155 | 13 | 52 | 7.90e-15 | 66.6 |
| MS.gene051831.t1 | AT5G57060 | 65.000 | 40 | 14 | 0 | 116 | 155 | 49 | 88 | 1.21e-14 | 67.4 |
| MS.gene051831.t1 | AT5G57060 | 65.000 | 40 | 14 | 0 | 116 | 155 | 49 | 88 | 1.21e-14 | 67.4 |
Find 34 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATGCTTTGGATGCGGAAT+TGG | 0.323771 | 8.2:+6906438 | MS.gene051831:CDS |
| GCAGCAGCAGCGGTCATTGC+CGG | 0.334404 | 8.2:+6901339 | MS.gene051831:CDS |
| GTCCTCTGGATCTCTAATTA+AGG | 0.363548 | 8.2:-6906385 | None:intergenic |
| GATAAGCCCCTCCCATGCTT+TGG | 0.389734 | 8.2:+6906426 | MS.gene051831:CDS |
| ATCAAAATTCTTAAAAGAAA+CGG | 0.429179 | 8.2:-6904005 | None:intergenic |
| CGGTCATTGCCGGCAACAAT+TGG | 0.434578 | 8.2:+6901349 | MS.gene051831:CDS |
| TTTAAGAAAGGGCGTGGAAT+CGG | 0.452413 | 8.2:+6903976 | MS.gene051831:CDS |
| TTTCCCTTTCCGTGAGCCAT+TGG | 0.452477 | 8.2:-6906357 | None:intergenic |
| GATCCAGAGGACTTCCAGTC+AGG | 0.454913 | 8.2:+6906396 | MS.gene051831:CDS |
| AGAGAATGTTGATTTAAGAA+AGG | 0.460736 | 8.2:+6903964 | MS.gene051831:CDS |
| GATCCAATGGCTCACGGAAA+GGG | 0.471290 | 8.2:+6906354 | MS.gene051831:CDS |
| GAGAATGTTGATTTAAGAAA+GGG | 0.481994 | 8.2:+6903965 | MS.gene051831:CDS |
| TGTTGATTTAAGAAAGGGCG+TGG | 0.497594 | 8.2:+6903970 | MS.gene051831:CDS |
| CTTTGTTGTTGCAGATGGTA+GGG | 0.498784 | 8.2:+6903937 | MS.gene051831:intron |
| AGTCCTGACTGGAAGTCCTC+TGG | 0.502119 | 8.2:-6906399 | None:intergenic |
| CCTTTGTTGTTGCAGATGGT+AGG | 0.504377 | 8.2:+6903936 | MS.gene051831:intron |
| CCAATTCCGCATCCAAAGCA+TGG | 0.518285 | 8.2:-6906438 | None:intergenic |
| CTTTATGTGGCAGGACAAGC+AGG | 0.542559 | 8.2:+6906330 | MS.gene051831:intron |
| CCCCTCCCATGCTTTGGATG+CGG | 0.543713 | 8.2:+6906432 | MS.gene051831:CDS |
| CTTGAATTACTCCGAGCAAA+GGG | 0.545855 | 8.2:-6904037 | None:intergenic |
| TTCCGCATCCAAAGCATGGG+AGG | 0.555498 | 8.2:-6906434 | None:intergenic |
| TCTTGAATTACTCCGAGCAA+AGG | 0.556216 | 8.2:-6904038 | None:intergenic |
| AGATCCAATGGCTCACGGAA+AGG | 0.578977 | 8.2:+6906353 | MS.gene051831:CDS |
| GCTTATCATAAAGTCCTGAC+TGG | 0.578995 | 8.2:-6906410 | None:intergenic |
| TCCGCATCCAAAGCATGGGA+GGG | 0.588988 | 8.2:-6906433 | None:intergenic |
| GCTTTGGATGCGGAATTGGA+TGG | 0.591155 | 8.2:+6906442 | MS.gene051831:CDS |
| CAATTCCGCATCCAAAGCAT+GGG | 0.593569 | 8.2:-6906437 | None:intergenic |
| GGCAAACTCAGCAGCAGCAG+CGG | 0.605297 | 8.2:+6901329 | MS.gene051831:CDS |
| CCGCATCCAAAGCATGGGAG+GGG | 0.606480 | 8.2:-6906432 | None:intergenic |
| AGGACAAGCAGGAGATCCAA+TGG | 0.609106 | 8.2:+6906341 | MS.gene051831:intron |
| TGCCTTAATTAGAGATCCAG+AGG | 0.616154 | 8.2:+6906383 | MS.gene051831:CDS |
| GCAGGAGATCCAATGGCTCA+CGG | 0.665431 | 8.2:+6906348 | MS.gene051831:CDS |
| TGTTGTTGCAGATGGTAGGG+TGG | 0.672021 | 8.2:+6903940 | MS.gene051831:intron |
| TTTGCTCGGAGTAATTCAAG+AGG | 0.694585 | 8.2:+6904040 | MS.gene051831:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 6901327 | 6909183 | 6901327 | ID=MS.gene051831 |
| chr8.2 | mRNA | 6901327 | 6909183 | 6901327 | ID=MS.gene051831.t1;Parent=MS.gene051831 |
| chr8.2 | exon | 6901327 | 6901549 | 6901327 | ID=MS.gene051831.t1.exon1;Parent=MS.gene051831.t1 |
| chr8.2 | CDS | 6901327 | 6901549 | 6901327 | ID=cds.MS.gene051831.t1;Parent=MS.gene051831.t1 |
| chr8.2 | exon | 6903951 | 6904061 | 6903951 | ID=MS.gene051831.t1.exon2;Parent=MS.gene051831.t1 |
| chr8.2 | CDS | 6903951 | 6904061 | 6903951 | ID=cds.MS.gene051831.t1;Parent=MS.gene051831.t1 |
| chr8.2 | exon | 6906343 | 6906469 | 6906343 | ID=MS.gene051831.t1.exon3;Parent=MS.gene051831.t1 |
| chr8.2 | CDS | 6906343 | 6906469 | 6906343 | ID=cds.MS.gene051831.t1;Parent=MS.gene051831.t1 |
| chr8.2 | exon | 6909177 | 6909183 | 6909177 | ID=MS.gene051831.t1.exon4;Parent=MS.gene051831.t1 |
| chr8.2 | CDS | 6909177 | 6909183 | 6909177 | ID=cds.MS.gene051831.t1;Parent=MS.gene051831.t1 |
| Gene Sequence |
| Protein sequence |
