Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25265.t1 | XP_019418075.1 | 69.6 | 112 | 31 | 1 | 1 | 112 | 1 | 109 | 2.10E-35 | 158.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25265.t1 | Q9LUV2 | 62.5 | 112 | 39 | 1 | 1 | 112 | 1 | 109 | 4.1e-32 | 138.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25265.t1 | A0A151TDN5 | 67.9 | 112 | 33 | 1 | 1 | 112 | 1 | 109 | 7.4e-35 | 156.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050811 | MS.gene25265 | -0.80484 | 1.78E-49 | -1.69E-46 |
| MS.gene054343 | MS.gene25265 | 0.904022 | 1.89E-79 | -1.69E-46 |
| MS.gene054344 | MS.gene25265 | 0.832416 | 9.57E-56 | -1.69E-46 |
| MS.gene056351 | MS.gene25265 | 0.823673 | 1.22E-53 | -1.69E-46 |
| MS.gene057196 | MS.gene25265 | 0.819968 | 8.79E-53 | -1.69E-46 |
| MS.gene059512 | MS.gene25265 | 0.826432 | 2.72E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25265.t1 | MTR_4g124920 | 76.190 | 105 | 22 | 1 | 8 | 112 | 3 | 104 | 1.23e-50 | 155 |
| MS.gene25265.t1 | MTR_4g124920 | 75.000 | 72 | 15 | 1 | 8 | 79 | 3 | 71 | 2.58e-30 | 103 |
| MS.gene25265.t1 | MTR_2g084985 | 36.893 | 103 | 60 | 2 | 9 | 111 | 8 | 105 | 1.77e-14 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25265.t1 | AT3G17210 | 62.500 | 112 | 39 | 1 | 1 | 112 | 1 | 109 | 2.50e-45 | 142 |
| MS.gene25265.t1 | AT3G17210 | 62.500 | 112 | 39 | 1 | 1 | 112 | 1 | 109 | 2.50e-45 | 142 |
| MS.gene25265.t1 | AT5G22580 | 34.021 | 97 | 59 | 2 | 9 | 105 | 7 | 98 | 8.25e-15 | 65.5 |
Find 25 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGTTCCTCAATCTTCTCT+TGG | 0.329948 | 8.2:-7903672 | None:intergenic |
| GATTCAGGTAACAAATAATA+AGG | 0.348275 | 8.2:-7903990 | None:intergenic |
| AATGAGTAAATCCTTGATTC+AGG | 0.352373 | 8.2:-7904005 | None:intergenic |
| TTACCAATGGAATGACTTCA+TGG | 0.420824 | 8.2:-7903731 | None:intergenic |
| ATTATTTGTTACCTGAATCA+AGG | 0.432767 | 8.2:+7903994 | MS.gene25265:CDS |
| AAAGTTGCTCGTTCCTTGCT+TGG | 0.439365 | 8.2:+7904101 | MS.gene25265:CDS |
| GAGTTCCTCAATCTTCTCTT+GGG | 0.443255 | 8.2:-7903671 | None:intergenic |
| GTAGTGAAGAACATAGTGAT+TGG | 0.457715 | 8.2:+7903628 | MS.gene25265:CDS |
| GAGGAAATGGAGGAAGCAAA+AGG | 0.502851 | 8.2:+7903604 | None:intergenic |
| TGGGATGAGATTGACGAGAT+TGG | 0.516286 | 8.2:-7903707 | None:intergenic |
| ATTCAAAGGTTAACGATGGT+GGG | 0.545431 | 8.2:-7904150 | None:intergenic |
| TCCTTGCTTGGAGAAAGTGC+TGG | 0.548785 | 8.2:+7904113 | MS.gene25265:CDS |
| CACCCATGAAGTCATTCCAT+TGG | 0.553232 | 8.2:+7903728 | MS.gene25265:CDS |
| TGAATCAACATTTGAGAGTG+TGG | 0.555452 | 8.2:+7904032 | MS.gene25265:CDS |
| TTTGCATACTCAACATGAGC+AGG | 0.558557 | 8.2:-7904081 | None:intergenic |
| GAAGATTGAGGAACTCATCA+AGG | 0.571882 | 8.2:+7903678 | MS.gene25265:CDS |
| GCCAGCACTTTCTCCAAGCA+AGG | 0.576667 | 8.2:-7904114 | None:intergenic |
| GATTCAAAGGTTAACGATGG+TGG | 0.578082 | 8.2:-7904151 | None:intergenic |
| GAGATTGACGAGATTGGCAA+AGG | 0.580515 | 8.2:-7903701 | None:intergenic |
| CAATGGAATGACTTCATGGG+TGG | 0.583790 | 8.2:-7903727 | None:intergenic |
| AATCTCCCAAGAGAAGATTG+AGG | 0.597731 | 8.2:+7903666 | MS.gene25265:CDS |
| ATGAGCTACATACTCTGCAA+TGG | 0.614733 | 8.2:-7904058 | None:intergenic |
| TACCAATGGAATGACTTCAT+GGG | 0.631729 | 8.2:-7903730 | None:intergenic |
| AATGGAATGACTTCATGGGT+GGG | 0.706119 | 8.2:-7903726 | None:intergenic |
| ACGGATTCAAAGGTTAACGA+TGG | 0.711712 | 8.2:-7904154 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTAAGTTTTTTAAGTTT+TGG | - | chr8.2:7903833-7903852 | None:intergenic | 10.0% |
| !!! | TATACATGTCTTATTTTTTT+AGG | + | chr8.2:7903950-7903969 | MS.gene25265:intron | 15.0% |
| ! | GATTCAGGTAACAAATAATA+AGG | - | chr8.2:7903993-7904012 | None:intergenic | 25.0% |
| !! | ATTATTTGTTACCTGAATCA+AGG | + | chr8.2:7903994-7904013 | MS.gene25265:CDS | 25.0% |
| !! | TTTAAGTTTCATCTTACCAA+TGG | - | chr8.2:7903747-7903766 | None:intergenic | 25.0% |
| AATGAGTAAATCCTTGATTC+AGG | - | chr8.2:7904008-7904027 | None:intergenic | 30.0% | |
| GTAGTGAAGAACATAGTGAT+TGG | + | chr8.2:7903628-7903647 | MS.gene25265:CDS | 35.0% | |
| TACCAATGGAATGACTTCAT+GGG | - | chr8.2:7903733-7903752 | None:intergenic | 35.0% | |
| TGAATCAACATTTGAGAGTG+TGG | + | chr8.2:7904032-7904051 | MS.gene25265:CDS | 35.0% | |
| TTACCAATGGAATGACTTCA+TGG | - | chr8.2:7903734-7903753 | None:intergenic | 35.0% | |
| !! | ATAGTCTAGACTGCTTTTGT+CGG | - | chr8.2:7903783-7903802 | None:intergenic | 35.0% |
| AATCTCCCAAGAGAAGATTG+AGG | + | chr8.2:7903666-7903685 | MS.gene25265:CDS | 40.0% | |
| AATGGAATGACTTCATGGGT+GGG | - | chr8.2:7903729-7903748 | None:intergenic | 40.0% | |
| ATGAGCTACATACTCTGCAA+TGG | - | chr8.2:7904061-7904080 | None:intergenic | 40.0% | |
| GAAGATTGAGGAACTCATCA+AGG | + | chr8.2:7903678-7903697 | MS.gene25265:CDS | 40.0% | |
| GAGTTCCTCAATCTTCTCTT+GGG | - | chr8.2:7903674-7903693 | None:intergenic | 40.0% | |
| TGAGTTCCTCAATCTTCTCT+TGG | - | chr8.2:7903675-7903694 | None:intergenic | 40.0% | |
| TTTGCATACTCAACATGAGC+AGG | - | chr8.2:7904084-7904103 | None:intergenic | 40.0% | |
| AAAGTTGCTCGTTCCTTGCT+TGG | + | chr8.2:7904101-7904120 | MS.gene25265:CDS | 45.0% | |
| CAATGGAATGACTTCATGGG+TGG | - | chr8.2:7903730-7903749 | None:intergenic | 45.0% | |
| CACCCATGAAGTCATTCCAT+TGG | + | chr8.2:7903728-7903747 | MS.gene25265:CDS | 45.0% | |
| GAGATTGACGAGATTGGCAA+AGG | - | chr8.2:7903704-7903723 | None:intergenic | 45.0% | |
| TGGGATGAGATTGACGAGAT+TGG | - | chr8.2:7903710-7903729 | None:intergenic | 45.0% | |
| ! | GATTTTCGCTAAGTGGACGA+GGG | - | chr8.2:7903869-7903888 | None:intergenic | 45.0% |
| ! | TACCAGCGATTTTCGCTAAG+TGG | - | chr8.2:7903876-7903895 | None:intergenic | 45.0% |
| GTCCACTTAGCGAAAATCGC+TGG | + | chr8.2:7903871-7903890 | MS.gene25265:intron | 50.0% | |
| ! | CGATTTTCGCTAAGTGGACG+AGG | - | chr8.2:7903870-7903889 | None:intergenic | 50.0% |
| ! | TCCTTGCTTGGAGAAAGTGC+TGG | + | chr8.2:7904113-7904132 | MS.gene25265:CDS | 50.0% |
| GCCAGCACTTTCTCCAAGCA+AGG | - | chr8.2:7904117-7904136 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 7903610 | 7904170 | 7903610 | ID=MS.gene25265 |
| chr8.2 | mRNA | 7903610 | 7904170 | 7903610 | ID=MS.gene25265.t1;Parent=MS.gene25265 |
| chr8.2 | exon | 7903610 | 7903749 | 7903610 | ID=MS.gene25265.t1.exon1;Parent=MS.gene25265.t1 |
| chr8.2 | CDS | 7903610 | 7903749 | 7903610 | ID=cds.MS.gene25265.t1;Parent=MS.gene25265.t1 |
| chr8.2 | exon | 7903972 | 7904170 | 7903972 | ID=MS.gene25265.t1.exon2;Parent=MS.gene25265.t1 |
| chr8.2 | CDS | 7903972 | 7904170 | 7903972 | ID=cds.MS.gene25265.t1;Parent=MS.gene25265.t1 |
| Gene Sequence |
| Protein sequence |
