Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25322.t1 | XP_013448123.1 | 63.4 | 71 | 23 | 1 | 1 | 68 | 276 | 346 | 2.30E-09 | 72.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25322.t1 | A0A072U8F6 | 63.4 | 71 | 23 | 1 | 1 | 68 | 276 | 346 | 1.7e-09 | 72.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049873 | MS.gene25322 | 0.811467 | 6.90E-51 | -1.69E-46 |
| MS.gene051957 | MS.gene25322 | 0.805535 | 1.27E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene25322 | 0.813193 | 2.90E-51 | -1.69E-46 |
| MS.gene053006 | MS.gene25322 | 0.812771 | 3.59E-51 | -1.69E-46 |
| MS.gene05548 | MS.gene25322 | 0.832131 | 1.13E-55 | -1.69E-46 |
| MS.gene057060 | MS.gene25322 | 0.820399 | 7.00E-53 | -1.69E-46 |
| MS.gene057693 | MS.gene25322 | 0.822407 | 2.41E-53 | -1.69E-46 |
| MS.gene05954 | MS.gene25322 | 0.812849 | 3.45E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 131 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTGATTAGATACATGTAT+TGG | - | 57419:19059-19078 | MS.gene25322:CDS | 20.0% |
| ! | AAATCCAATGAATGATTATC+CGG | - | 57419:17991-18010 | MS.gene25322:CDS | 25.0% |
| ! | ATTACTGTGTAAAGAAAACT+AGG | - | 57419:18455-18474 | MS.gene25322:CDS | 25.0% |
| ! | CATATGTATATAATCTAACC+CGG | + | 57419:18597-18616 | None:intergenic | 25.0% |
| ! | GAAGAAGAAGAAAAAAATGA+AGG | + | 57419:18388-18407 | None:intergenic | 25.0% |
| !! | ATTTTTATATACGACGAGAT+TGG | + | 57419:18493-18512 | None:intergenic | 25.0% |
| !! | GAAAAATGTTTTTCCTTTTC+CGG | - | 57419:18291-18310 | MS.gene25322:intron | 25.0% |
| AAACAATGTCAGCTAAATAC+CGG | + | 57419:18313-18332 | None:intergenic | 30.0% | |
| TCAAAATCCTCATCATACAA+TGG | + | 57419:18157-18176 | None:intergenic | 30.0% | |
| TCTCGTCGTATATAAAAATC+AGG | - | 57419:18494-18513 | MS.gene25322:intron | 30.0% | |
| TGAATTGTCAGATAATGATG+AGG | - | 57419:18084-18103 | MS.gene25322:CDS | 30.0% | |
| TGGATATGATAGGATATTCT+GGG | - | 57419:18419-18438 | MS.gene25322:CDS | 30.0% | |
| TTACACAGTAATATCCTGTT+AGG | + | 57419:18447-18466 | None:intergenic | 30.0% | |
| ! | CGGGTTAGATTATATACATA+TGG | - | 57419:18595-18614 | MS.gene25322:intron | 30.0% |
| ! | TTTGATGATTATGAAGGTCA+AGG | - | 57419:18172-18191 | MS.gene25322:intron | 30.0% |
| !! | GAGGATTTTGATGATTATGA+AGG | - | 57419:18166-18185 | MS.gene25322:intron | 30.0% |
| !! | TCATTTAGACTTTTTCCGAT+TGG | - | 57419:19084-19103 | MS.gene25322:CDS | 30.0% |
| AATGATGAGGATGAAGATGT+GGG | - | 57419:18208-18227 | MS.gene25322:intron | 35.0% | |
| ATGTGGGTAATGAACATTGA+AGG | - | 57419:18224-18243 | MS.gene25322:intron | 35.0% | |
| CGAATTTATGAATACCCAGA+TGG | + | 57419:18267-18286 | None:intergenic | 35.0% | |
| CTATCCAGATCGAATATAGT+CGG | + | 57419:19172-19191 | None:intergenic | 35.0% | |
| GTGGATATGATAGGATATTC+TGG | - | 57419:18418-18437 | MS.gene25322:CDS | 35.0% | |
| TCAAAGTGTGTGGATATGAT+AGG | - | 57419:18409-18428 | MS.gene25322:CDS | 35.0% | |
| TTATATACGACGAGATTGGA+GGG | + | 57419:18489-18508 | None:intergenic | 35.0% | |
| TTCTGATCCATTGTATGATG+AGG | - | 57419:18147-18166 | MS.gene25322:intron | 35.0% | |
| TTCTTCTTCTTCAAAGTGTG+TGG | - | 57419:18399-18418 | MS.gene25322:CDS | 35.0% | |
| TTTATATACGACGAGATTGG+AGG | + | 57419:18490-18509 | None:intergenic | 35.0% | |
| ! | GTCTGTATGTTTTGTGATGT+TGG | - | 57419:18848-18867 | MS.gene25322:intron | 35.0% |
| ! | TGTATGTTTTGTGATGTTGG+AGG | - | 57419:18851-18870 | MS.gene25322:intron | 35.0% |
| ! | TTGATGGTTGTTTATTCCGA+TGG | - | 57419:19121-19140 | MS.gene25322:CDS | 35.0% |
| !! | GTTCTGTTGCTGTTTATGTT+GGG | - | 57419:18986-19005 | MS.gene25322:CDS | 35.0% |
| !! | TGTTCTGTTGCTGTTTATGT+TGG | - | 57419:18985-19004 | MS.gene25322:CDS | 35.0% |
| !! | TGTTGACTTGAACGAGTAAT+GGG | - | 57419:18347-18366 | MS.gene25322:intron | 35.0% |
| !! | TTGTTGACTTGAACGAGTAA+TGG | - | 57419:18346-18365 | MS.gene25322:intron | 35.0% |
| !! | TTTAGACTTTTTCCGATTGG+AGG | - | 57419:19087-19106 | MS.gene25322:CDS | 35.0% |
| AGATCTACTTGGTTGAGGTA+AGG | - | 57419:18677-18696 | MS.gene25322:intron | 40.0% | |
| ATGAATAGCACCACCGAAAA+CGG | + | 57419:18909-18928 | None:intergenic | 40.0% | |
| CAATGATGAGGATGAAGATG+TGG | - | 57419:18207-18226 | MS.gene25322:intron | 40.0% | |
| CGTATATACCAGGATTTGTC+CGG | - | 57419:18575-18594 | MS.gene25322:intron | 40.0% | |
| CTGTTTATGTTGGGAAGTTG+TGG | - | 57419:18995-19014 | MS.gene25322:CDS | 40.0% | |
| GAGATTTCGGAAGATGAAGA+AGG | - | 57419:18016-18035 | MS.gene25322:CDS | 40.0% | |
| GATTATGAAGGTCAAGGTGT+AGG | - | 57419:18178-18197 | MS.gene25322:intron | 40.0% | |
| GTATATACCAGGATTTGTCC+GGG | - | 57419:18576-18595 | MS.gene25322:intron | 40.0% | |
| GTCAGATAATGATGAGGATG+TGG | - | 57419:18090-18109 | MS.gene25322:intron | 40.0% | |
| TACAGACACCAACACAAATC+CGG | + | 57419:18835-18854 | None:intergenic | 40.0% | |
| TCAGAAGAAAGCAAGCATAG+CGG | - | 57419:18052-18071 | MS.gene25322:CDS | 40.0% | |
| TCGTCCGGATAATCATTCAT+TGG | + | 57419:17998-18017 | None:intergenic | 40.0% | |
| TGATCTGCTTCAGATCTACT+TGG | - | 57419:18666-18685 | MS.gene25322:intron | 40.0% | |
| TGATTATCCGGACGAGATTT+CGG | - | 57419:18003-18022 | MS.gene25322:CDS | 40.0% | |
| TGGGTAATGAACATTGAAGG+TGG | - | 57419:18227-18246 | MS.gene25322:intron | 40.0% | |
| TGTCAGCTAAATACCGGAAA+AGG | + | 57419:18307-18326 | None:intergenic | 40.0% | |
| TTCACACTAGCGTATATACC+AGG | - | 57419:18565-18584 | MS.gene25322:intron | 40.0% | |
| ! | AACATTGAAGGTGGTCTTAC+CGG | - | 57419:18236-18255 | MS.gene25322:CDS | 40.0% |
| ! | GTTCACATCCTTTTTGTTCC+CGG | - | 57419:18619-18638 | MS.gene25322:intron | 40.0% |
| ! | TGTAGGCTTTGACAATGATG+AGG | - | 57419:18195-18214 | MS.gene25322:intron | 40.0% |
| !! | GAACTTCGTCGTTTTGCTAA+AGG | - | 57419:18118-18137 | MS.gene25322:intron | 40.0% |
| AAAAACACGCACAGAGAGAC+AGG | + | 57419:19043-19062 | None:intergenic | 45.0% | |
| AATCTAACCCGGACAAATCC+TGG | + | 57419:18586-18605 | None:intergenic | 45.0% | |
| AGATCAGAACTCACCCAAAC+TGG | + | 57419:18652-18671 | None:intergenic | 45.0% | |
| ATGTTGGAGGCTATTCCTGT+TGG | - | 57419:18864-18883 | MS.gene25322:intron | 45.0% | |
| GATCTGTCAAAGGTTCCGTT+TGG | - | 57419:18761-18780 | MS.gene25322:intron | 45.0% | |
| GCTTCAGATCTACTTGGTTG+AGG | - | 57419:18672-18691 | MS.gene25322:intron | 45.0% | |
| GGATATTCTGGGTTCCTAAC+AGG | - | 57419:18430-18449 | MS.gene25322:CDS | 45.0% | |
| TCATCTTCCGAAATCTCGTC+CGG | + | 57419:18013-18032 | None:intergenic | 45.0% | |
| TCTACTTGGTTGAGGTAAGG+TGG | - | 57419:18680-18699 | MS.gene25322:intron | 45.0% | |
| TGCTATTCATGGCTGTTTGG+TGG | - | 57419:18919-18938 | MS.gene25322:intron | 45.0% | |
| ! | ATGGCTGTTTGGTGGTTGTA+AGG | - | 57419:18927-18946 | MS.gene25322:intron | 45.0% |
| ! | GGTTAGCTGGTGGTATTCTA+TGG | - | 57419:19016-19035 | MS.gene25322:CDS | 45.0% |
| ! | TTTTTCCGATTGGAGGTGGA+TGG | - | 57419:19094-19113 | MS.gene25322:CDS | 45.0% |
| !! | AGACTTTTTCCGATTGGAGG+TGG | - | 57419:19090-19109 | MS.gene25322:CDS | 45.0% |
| !! | TGGTGCTATTCATGGCTGTT+TGG | - | 57419:18916-18935 | MS.gene25322:intron | 45.0% |
| !!! | GTTTTCGGTGGTGCTATTCA+TGG | - | 57419:18908-18927 | MS.gene25322:intron | 45.0% |
| AACCACCATCCACCTCCAAT+CGG | + | 57419:19102-19121 | None:intergenic | 50.0% | |
| ACTTGGTTGAGGTAAGGTGG+TGG | - | 57419:18683-18702 | MS.gene25322:intron | 50.0% | |
| AGAACTCACCCAAACTGGAC+AGG | + | 57419:18647-18666 | None:intergenic | 50.0% | |
| CCTCACAAGAGACCTCCAAA+CGG | + | 57419:18779-18798 | None:intergenic | 50.0% | |
| CTGTCAAAGGTTCCGTTTGG+AGG | - | 57419:18764-18783 | MS.gene25322:intron | 50.0% | |
| GAATACCCAGATGGCTTCAC+CGG | + | 57419:18258-18277 | None:intergenic | 50.0% | |
| GACAGGAACCGGGAACAAAA+AGG | + | 57419:18630-18649 | None:intergenic | 50.0% | |
| GTTGGGAAGTTGTGGTTAGC+TGG | - | 57419:19003-19022 | MS.gene25322:CDS | 50.0% | |
| TCTTACCGGTGAAGCCATCT+GGG | - | 57419:18250-18269 | MS.gene25322:CDS | 50.0% | |
| TTTGGAGGTCTCTTGTGAGG+TGG | - | 57419:18779-18798 | MS.gene25322:intron | 50.0% | |
| ! | ATTGGTGTCCAGGTTGTGTG+AGG | - | 57419:18723-18742 | MS.gene25322:intron | 50.0% |
| ! | GCTGTTTGGTGGTTGTAAGG+TGG | - | 57419:18930-18949 | MS.gene25322:intron | 50.0% |
| ! | GGAGGCTATTCCTGTTGGTT+TGG | - | 57419:18869-18888 | MS.gene25322:intron | 50.0% |
| !! | ACATATGGGAAGGCGTCCAT+CGG | + | 57419:19140-19159 | None:intergenic | 50.0% |
| !! | GGCTATTCCTGTTGGTTTGG+TGG | - | 57419:18872-18891 | MS.gene25322:intron | 50.0% |
| !! | TGTGGTGGTGGATCTGTCAA+AGG | - | 57419:18751-18770 | MS.gene25322:intron | 50.0% |
| !!! | GCGGTTTTGATGGTGAGGAT+TGG | - | 57419:18705-18724 | MS.gene25322:intron | 50.0% |
| !!! | TAAGGTGGTGGCGGTTTTGA+TGG | - | 57419:18695-18714 | MS.gene25322:intron | 50.0% |
| ACCCAAACTGGACAGGAACC+GGG | + | 57419:18640-18659 | None:intergenic | 55.0% | |
| ATAGCACCACCGAAAACGGC+AGG | + | 57419:18905-18924 | None:intergenic | 55.0% | |
| CACCCAAACTGGACAGGAAC+CGG | + | 57419:18641-18660 | None:intergenic | 55.0% | |
| CCGTTTGGAGGTCTCTTGTG+AGG | - | 57419:18776-18795 | MS.gene25322:intron | 55.0% | |
| GACGCCTTCCCATATGTCAC+CGG | - | 57419:19143-19162 | MS.gene25322:CDS | 55.0% | |
| GAGGTGGTCCGGATTTGTGT+TGG | - | 57419:18824-18843 | MS.gene25322:intron | 55.0% | |
| GATCGAATATAGTCGGCCGC+CGG | + | 57419:19165-19184 | None:intergenic | 55.0% | |
| GCGGCCGACTATATTCGATC+TGG | - | 57419:19165-19184 | MS.gene25322:CDS | 55.0% | |
| GTCTTACCGGTGAAGCCATC+TGG | - | 57419:18249-18268 | MS.gene25322:CDS | 55.0% | |
| TCCCGGTTCCTGTCCAGTTT+GGG | - | 57419:18636-18655 | MS.gene25322:intron | 55.0% | |
| TGGTGGAGACATGTGGTGGT+GGG | - | 57419:18802-18821 | MS.gene25322:intron | 55.0% | |
| TGGTGGTTGTAAGGTGGTGC+TGG | - | 57419:18936-18955 | MS.gene25322:intron | 55.0% | |
| TTCCCGGTTCCTGTCCAGTT+TGG | - | 57419:18635-18654 | MS.gene25322:intron | 55.0% | |
| TTCCGATTGGAGGTGGATGG+TGG | - | 57419:19097-19116 | MS.gene25322:CDS | 55.0% | |
| ! | GATGGTGAGGATTGGTGTCC+AGG | - | 57419:18713-18732 | MS.gene25322:intron | 55.0% |
| ! | GGAGGTGGATGGTGGTTTGA+TGG | - | 57419:19105-19124 | MS.gene25322:CDS | 55.0% |
| ! | GGGAAGTTGTGGTTAGCTGG+TGG | - | 57419:19006-19025 | MS.gene25322:CDS | 55.0% |
| ! | TGGTTGAGGTAAGGTGGTGG+CGG | - | 57419:18686-18705 | MS.gene25322:intron | 55.0% |
| !! | TGTAAGGTGGTGCTGGCGTT+TGG | - | 57419:18943-18962 | MS.gene25322:intron | 55.0% |
| !!! | TGGTGGCGGTTTTGATGGTG+AGG | - | 57419:18700-18719 | MS.gene25322:intron | 55.0% |
| GACAGATCCACCACCACAGC+CGG | + | 57419:18749-18768 | None:intergenic | 60.0% | |
| GCCTTCCCATATGTCACCGG+CGG | - | 57419:19146-19165 | MS.gene25322:CDS | 60.0% | |
| GGAGGTCTCTTGTGAGGTGG+TGG | - | 57419:18782-18801 | MS.gene25322:intron | 60.0% | |
| GTGTCCAGGTTGTGTGAGGC+CGG | - | 57419:18727-18746 | MS.gene25322:intron | 60.0% | |
| TCTCGCGCCACCAAACCAAC+AGG | + | 57419:18882-18901 | None:intergenic | 60.0% | |
| ! | GGTCTCTTGTGAGGTGGTGG+TGG | - | 57419:18785-18804 | MS.gene25322:intron | 60.0% |
| ! | GTGGTGGAGACATGTGGTGG+TGG | - | 57419:18801-18820 | MS.gene25322:intron | 60.0% |
| !! | AGACATGTGGTGGTGGGAGG+TGG | - | 57419:18808-18827 | MS.gene25322:intron | 60.0% |
| !! | GAGGTGGTGGTGGAGACATG+TGG | - | 57419:18795-18814 | MS.gene25322:intron | 60.0% |
| !! | GTGGTGGTGGAGACATGTGG+TGG | - | 57419:18798-18817 | MS.gene25322:intron | 60.0% |
| !! | TGGAGACATGTGGTGGTGGG+AGG | - | 57419:18805-18824 | MS.gene25322:intron | 60.0% |
| ACAGCCGGCCTCACACAACC+TGG | + | 57419:18734-18753 | None:intergenic | 65.0% | |
| AGGTTGTGTGAGGCCGGCTG+TGG | - | 57419:18733-18752 | MS.gene25322:intron | 65.0% | |
| AGTCGGCCGCCGGTGACATA+TGG | + | 57419:19155-19174 | None:intergenic | 65.0% | |
| GCCGCCGGTGACATATGGGA+AGG | + | 57419:19150-19169 | None:intergenic | 65.0% | |
| GTCGGCCGCCGGTGACATAT+GGG | + | 57419:19154-19173 | None:intergenic | 65.0% | |
| TTGTGTGAGGCCGGCTGTGG+TGG | - | 57419:18736-18755 | MS.gene25322:intron | 65.0% | |
| !! | TGTGGTGGTGGGAGGTGGTC+CGG | - | 57419:18813-18832 | MS.gene25322:intron | 65.0% |
| !!! | GCGAGACCTGCCGTTTTCGG+TGG | - | 57419:18896-18915 | MS.gene25322:intron | 65.0% |
| !!! | GGCGCGAGACCTGCCGTTTT+CGG | - | 57419:18893-18912 | MS.gene25322:intron | 65.0% |
| TGTGAGGCCGGCTGTGGTGG+TGG | - | 57419:18739-18758 | MS.gene25322:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57419 | gene | 17984 | 19191 | 17984 | ID=MS.gene25322 |
| 57419 | mRNA | 17984 | 19191 | 17984 | ID=MS.gene25322.t1;Parent=MS.gene25322 |
| 57419 | exon | 18946 | 19191 | 18946 | ID=MS.gene25322.t1.exon1;Parent=MS.gene25322.t1 |
| 57419 | CDS | 18946 | 19191 | 18946 | ID=cds.MS.gene25322.t1;Parent=MS.gene25322.t1 |
| 57419 | exon | 18390 | 18476 | 18390 | ID=MS.gene25322.t1.exon2;Parent=MS.gene25322.t1 |
| 57419 | CDS | 18390 | 18476 | 18390 | ID=cds.MS.gene25322.t1;Parent=MS.gene25322.t1 |
| 57419 | exon | 18232 | 18302 | 18232 | ID=MS.gene25322.t1.exon3;Parent=MS.gene25322.t1 |
| 57419 | CDS | 18232 | 18302 | 18232 | ID=cds.MS.gene25322.t1;Parent=MS.gene25322.t1 |
| 57419 | exon | 17984 | 18106 | 17984 | ID=MS.gene25322.t1.exon4;Parent=MS.gene25322.t1 |
| 57419 | CDS | 17984 | 18106 | 17984 | ID=cds.MS.gene25322.t1;Parent=MS.gene25322.t1 |
| Gene Sequence |
| Protein sequence |
