Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25536.t1 | XP_013445649.1 | 97.4 | 115 | 3 | 0 | 1 | 115 | 1 | 115 | 5.20E-58 | 233.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25536.t1 | O81831 | 62.3 | 114 | 42 | 1 | 3 | 115 | 11 | 124 | 7.7e-34 | 144.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25536.t1 | A0A072U1J6 | 97.4 | 115 | 3 | 0 | 1 | 115 | 1 | 115 | 3.8e-58 | 233.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058526 | MS.gene25536 | 0.81285 | 3.44E-51 | -1.69E-46 |
| MS.gene058527 | MS.gene25536 | 0.817128 | 3.87E-52 | -1.69E-46 |
| MS.gene058528 | MS.gene25536 | 0.814798 | 1.28E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene067296 | MS.gene25536 | PPI |
| MS.gene84736 | MS.gene25536 | PPI |
| MS.gene068384 | MS.gene25536 | PPI |
| MS.gene060524 | MS.gene25536 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25536.t1 | MTR_8g466250 | 97.391 | 115 | 3 | 0 | 1 | 115 | 1 | 115 | 2.14e-78 | 226 |
| MS.gene25536.t1 | MTR_2g081580 | 77.193 | 114 | 25 | 1 | 1 | 114 | 1 | 113 | 2.23e-58 | 176 |
| MS.gene25536.t1 | MTR_1g052885 | 66.667 | 105 | 35 | 0 | 11 | 115 | 6 | 110 | 2.34e-45 | 142 |
| MS.gene25536.t1 | MTR_2g089210 | 65.714 | 105 | 36 | 0 | 11 | 115 | 10 | 114 | 4.94e-44 | 139 |
| MS.gene25536.t1 | MTR_2g011400 | 59.091 | 110 | 45 | 0 | 2 | 111 | 5 | 114 | 1.06e-39 | 129 |
| MS.gene25536.t1 | MTR_2g081300 | 66.667 | 96 | 31 | 1 | 11 | 106 | 5 | 99 | 4.51e-39 | 127 |
| MS.gene25536.t1 | MTR_8g027180 | 50.000 | 116 | 55 | 1 | 3 | 115 | 8 | 123 | 9.76e-38 | 124 |
| MS.gene25536.t1 | MTR_2g081350 | 64.583 | 96 | 33 | 1 | 11 | 106 | 9 | 103 | 2.42e-37 | 122 |
| MS.gene25536.t1 | MTR_2g081440 | 61.458 | 96 | 36 | 1 | 11 | 106 | 9 | 103 | 5.44e-35 | 116 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25536.t1 | AT4G27280 | 62.281 | 114 | 42 | 1 | 3 | 115 | 11 | 124 | 5.20e-46 | 145 |
| MS.gene25536.t1 | AT5G54490 | 57.895 | 114 | 47 | 1 | 3 | 115 | 11 | 124 | 1.34e-42 | 136 |
| MS.gene25536.t1 | AT2G46600 | 50.000 | 114 | 57 | 0 | 2 | 115 | 13 | 126 | 1.27e-37 | 124 |
Find 28 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATGATCACCTTCCTTTCT+TGG | 0.113224 | 8.2:-43096436 | MS.gene25536:CDS |
| TCAGCTTTGTTGGGATTAAA+TGG | 0.221597 | 8.2:-43096306 | MS.gene25536:CDS |
| TTATGGAGGGCTCTAAATTA+TGG | 0.295918 | 8.2:-43096173 | MS.gene25536:CDS |
| TAGATTGAGCCCTGAACTTA+TGG | 0.297254 | 8.2:-43096190 | MS.gene25536:CDS |
| CCTTTCTTGGCCAACAAGTT+AGG | 0.305684 | 8.2:-43096423 | MS.gene25536:CDS |
| GAAAATGAATTCAGCTTTGT+TGG | 0.357480 | 8.2:-43096316 | MS.gene25536:CDS |
| TTAGAGCCCTCCATAAGTTC+AGG | 0.400767 | 8.2:+43096180 | None:intergenic |
| AAAATGAATTCAGCTTTGTT+GGG | 0.421549 | 8.2:-43096315 | MS.gene25536:CDS |
| GCCATCTCCACCTAACTTGT+TGG | 0.424644 | 8.2:+43096413 | None:intergenic |
| CCTAACTTGTTGGCCAAGAA+AGG | 0.452388 | 8.2:+43096423 | None:intergenic |
| TGATAGATGAACTATGTAAT+GGG | 0.463953 | 8.2:-43096389 | MS.gene25536:CDS |
| AAAATCAAAATTGATGTTGA+AGG | 0.466207 | 8.2:+43096139 | None:intergenic |
| TCAAGACACAGAACTCCATT+TGG | 0.470697 | 8.2:+43096217 | None:intergenic |
| CCAAGAAAGGAAGGTGATCA+TGG | 0.477663 | 8.2:+43096436 | None:intergenic |
| AGATGGTGCTCTTAACCAAA+TGG | 0.509208 | 8.2:-43096232 | MS.gene25536:CDS |
| TAGAGCCCTCCATAAGTTCA+GGG | 0.512725 | 8.2:+43096181 | None:intergenic |
| GGAGGGCTCTAAATTATGGT+TGG | 0.516487 | 8.2:-43096169 | MS.gene25536:CDS |
| TTGATAGATGAACTATGTAA+TGG | 0.526523 | 8.2:-43096390 | MS.gene25536:CDS |
| GCCAACAAGTTAGGTGGAGA+TGG | 0.528877 | 8.2:-43096414 | MS.gene25536:CDS |
| CTCTAAATTATGGTTGGAAG+AGG | 0.543366 | 8.2:-43096163 | MS.gene25536:CDS |
| AAATCAAAATTGATGTTGAA+GGG | 0.549437 | 8.2:+43096140 | None:intergenic |
| TTCTTGGCCAACAAGTTAGG+TGG | 0.555886 | 8.2:-43096420 | MS.gene25536:CDS |
| ACTTGTTGGCCAAGAAAGGA+AGG | 0.577294 | 8.2:+43096427 | None:intergenic |
| ATTGAGCCCTGAACTTATGG+AGG | 0.584540 | 8.2:-43096187 | MS.gene25536:CDS |
| TTGAGCCCTGAACTTATGGA+GGG | 0.638673 | 8.2:-43096186 | MS.gene25536:CDS |
| CATGGAAATGAGACTGTGGT+GGG | 0.688196 | 8.2:+43096454 | None:intergenic |
| TCATGGAAATGAGACTGTGG+TGG | 0.705310 | 8.2:+43096453 | None:intergenic |
| TGATCATGGAAATGAGACTG+TGG | 0.746765 | 8.2:+43096450 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGATAGATGAACTATGTAAT+GGG | - | chr8.2:43096222-43096241 | MS.gene25536:CDS | 25.0% |
| ! | TTGATAGATGAACTATGTAA+TGG | - | chr8.2:43096221-43096240 | MS.gene25536:CDS | 25.0% |
| !!! | AAAATGAATTCAGCTTTGTT+GGG | - | chr8.2:43096296-43096315 | MS.gene25536:CDS | 25.0% |
| !!! | CTTTTGAAGGATTCTAATAA+AGG | - | chr8.2:43096251-43096270 | MS.gene25536:CDS | 25.0% |
| ! | ACAAAGCTGAATTCATTTTC+AGG | + | chr8.2:43096295-43096314 | None:intergenic | 30.0% |
| ! | AGATCTTTTGTCTATGCTAA+TGG | - | chr8.2:43096340-43096359 | MS.gene25536:CDS | 30.0% |
| ! | GAAAATGAATTCAGCTTTGT+TGG | - | chr8.2:43096295-43096314 | MS.gene25536:CDS | 30.0% |
| !!! | TAATGGGTTCAATCTTTTGA+AGG | - | chr8.2:43096238-43096257 | MS.gene25536:CDS | 30.0% |
| CTCTAAATTATGGTTGGAAG+AGG | - | chr8.2:43096448-43096467 | MS.gene25536:CDS | 35.0% | |
| ! | CTAATGGAAGGTGATTTTGA+TGG | - | chr8.2:43096356-43096375 | MS.gene25536:CDS | 35.0% |
| ! | CTTTTGTCTATGCTAATGGA+AGG | - | chr8.2:43096344-43096363 | MS.gene25536:CDS | 35.0% |
| !! | TCAGCTTTGTTGGGATTAAA+TGG | - | chr8.2:43096305-43096324 | MS.gene25536:CDS | 35.0% |
| !! | TTATGGAGGGCTCTAAATTA+TGG | - | chr8.2:43096438-43096457 | MS.gene25536:CDS | 35.0% |
| AGATGGTGCTCTTAACCAAA+TGG | - | chr8.2:43096379-43096398 | MS.gene25536:CDS | 40.0% | |
| TAGATTGAGCCCTGAACTTA+TGG | - | chr8.2:43096421-43096440 | MS.gene25536:CDS | 40.0% | |
| TCAAGACACAGAACTCCATT+TGG | + | chr8.2:43096397-43096416 | None:intergenic | 40.0% | |
| TGATCATGGAAATGAGACTG+TGG | + | chr8.2:43096164-43096183 | None:intergenic | 40.0% | |
| !! | GAAGGTGATTTTGATGGAGA+TGG | - | chr8.2:43096362-43096381 | MS.gene25536:CDS | 40.0% |
| ATTGAGCCCTGAACTTATGG+AGG | - | chr8.2:43096424-43096443 | MS.gene25536:CDS | 45.0% | |
| CATGGAAATGAGACTGTGGT+GGG | + | chr8.2:43096160-43096179 | None:intergenic | 45.0% | |
| CCAAGAAAGGAAGGTGATCA+TGG | + | chr8.2:43096178-43096197 | None:intergenic | 45.0% | |
| CCATGATCACCTTCCTTTCT+TGG | - | chr8.2:43096175-43096194 | MS.gene25536:CDS | 45.0% | |
| CCTTTCTTGGCCAACAAGTT+AGG | - | chr8.2:43096188-43096207 | MS.gene25536:CDS | 45.0% | |
| TAGAGCCCTCCATAAGTTCA+GGG | + | chr8.2:43096433-43096452 | None:intergenic | 45.0% | |
| TCATGGAAATGAGACTGTGG+TGG | + | chr8.2:43096161-43096180 | None:intergenic | 45.0% | |
| TTAGAGCCCTCCATAAGTTC+AGG | + | chr8.2:43096434-43096453 | None:intergenic | 45.0% | |
| TTCTTGGCCAACAAGTTAGG+TGG | - | chr8.2:43096191-43096210 | MS.gene25536:CDS | 45.0% | |
| TTGAGCCCTGAACTTATGGA+GGG | - | chr8.2:43096425-43096444 | MS.gene25536:CDS | 45.0% | |
| ! | ACTTGTTGGCCAAGAAAGGA+AGG | + | chr8.2:43096187-43096206 | None:intergenic | 45.0% |
| !! | CCTAACTTGTTGGCCAAGAA+AGG | + | chr8.2:43096191-43096210 | None:intergenic | 45.0% |
| !! | GGAGGGCTCTAAATTATGGT+TGG | - | chr8.2:43096442-43096461 | MS.gene25536:CDS | 45.0% |
| GCCAACAAGTTAGGTGGAGA+TGG | - | chr8.2:43096197-43096216 | MS.gene25536:CDS | 50.0% | |
| ! | GCCATCTCCACCTAACTTGT+TGG | + | chr8.2:43096201-43096220 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 43096143 | 43096490 | 43096143 | ID=MS.gene25536 |
| chr8.2 | mRNA | 43096143 | 43096490 | 43096143 | ID=MS.gene25536.t1;Parent=MS.gene25536 |
| chr8.2 | exon | 43096143 | 43096490 | 43096143 | ID=MS.gene25536.t1.exon1;Parent=MS.gene25536.t1 |
| chr8.2 | CDS | 43096143 | 43096490 | 43096143 | ID=cds.MS.gene25536.t1;Parent=MS.gene25536.t1 |
| Gene Sequence |
| Protein sequence |
