Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25643.t1 | XP_003609992.1 | 97.4 | 190 | 5 | 0 | 1 | 190 | 1 | 190 | 1.20E-104 | 389 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25643.t1 | G7JT65 | 97.4 | 190 | 5 | 0 | 1 | 190 | 1 | 190 | 8.9e-105 | 389.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049807 | MS.gene25643 | 0.824533 | 7.67E-54 | -1.69E-46 |
| MS.gene049808 | MS.gene25643 | 0.802202 | 6.27E-49 | -1.69E-46 |
| MS.gene049887 | MS.gene25643 | 0.81146 | 6.93E-51 | -1.69E-46 |
| MS.gene053144 | MS.gene25643 | 0.842178 | 3.03E-58 | -1.69E-46 |
| MS.gene054584 | MS.gene25643 | 0.835553 | 1.57E-56 | -1.69E-46 |
| MS.gene055468 | MS.gene25643 | 0.800878 | 1.17E-48 | -1.69E-46 |
| MS.gene055580 | MS.gene25643 | -0.816472 | 5.43E-52 | -1.69E-46 |
| MS.gene056043 | MS.gene25643 | -0.8247 | 7.00E-54 | -1.69E-46 |
| MS.gene060603 | MS.gene25643 | 0.814523 | 1.48E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25643.t1 | MTR_4g125100 | 97.368 | 190 | 5 | 0 | 1 | 190 | 1 | 190 | 3.49e-138 | 383 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25643.t1 | AT1G19130 | 67.725 | 189 | 58 | 3 | 3 | 190 | 6 | 192 | 4.17e-89 | 259 |
Find 38 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAAATCCACCTTCTGGATT+AGG | 0.309395 | 8.2:+7541596 | None:intergenic |
| TCCACGTAAAATCCACCTTC+TGG | 0.337620 | 8.2:+7541590 | None:intergenic |
| AGTGTTAAATTTACATGTCT+TGG | 0.348435 | 8.2:-7540557 | MS.gene25643:CDS |
| GAAACTCATTACTCTCTTGT+AGG | 0.414889 | 8.2:-7540401 | MS.gene25643:CDS |
| ATATACTTTCTGCTTCCATC+TGG | 0.431150 | 8.2:-7541406 | MS.gene25643:CDS |
| ACACGGTGCCTCCGAATGTC+TGG | 0.437212 | 8.2:-7540502 | MS.gene25643:CDS |
| GTAGGATGCACTTGTGCACC+TGG | 0.437438 | 8.2:-7540383 | MS.gene25643:CDS |
| GTGCCTCCGAATGTCTGGTT+TGG | 0.451216 | 8.2:-7540497 | MS.gene25643:CDS |
| AACAAAGGAATTACGGTGAT+AGG | 0.454551 | 8.2:+7541322 | None:intergenic |
| GTTTAGCAAGTTCAAAATCC+TGG | 0.459347 | 8.2:+7540351 | None:intergenic |
| ATCAGAAAGAATGTCTTTGT+CGG | 0.473073 | 8.2:-7541651 | None:intergenic |
| TGATGCCATGTTTCAGAAGA+AGG | 0.497508 | 8.2:+7541358 | None:intergenic |
| AAAGAACCAAACCAGACATT+CGG | 0.516467 | 8.2:+7540491 | None:intergenic |
| GAAACATGGCATCATTACAT+TGG | 0.519533 | 8.2:-7541349 | MS.gene25643:CDS |
| TCTTGGATCTGATCTAACTA+AGG | 0.525817 | 8.2:-7540540 | MS.gene25643:CDS |
| CAGAAGAAGGAATATGATGA+AGG | 0.540545 | 8.2:+7541371 | None:intergenic |
| TTTACTTACATTCAGGTGGA+AGG | 0.547646 | 8.2:+7541528 | None:intergenic |
| TTTGCTGTCTCTGCAGATGG+GGG | 0.555085 | 8.2:-7540455 | MS.gene25643:CDS |
| AAGTATATGCAGGTCGACAC+TGG | 0.558993 | 8.2:+7541421 | None:intergenic |
| ATATTCCTTCTTCTGAAACA+TGG | 0.566858 | 8.2:-7541363 | MS.gene25643:CDS |
| GTGACAAGAGACTCCAATTG+AGG | 0.568370 | 8.2:+7540305 | None:intergenic |
| TTGCTGTCTCTGCAGATGGG+GGG | 0.568555 | 8.2:-7540454 | MS.gene25643:CDS |
| TGCAGAGACAGCAAAATCAC+TGG | 0.576800 | 8.2:+7540463 | None:intergenic |
| ATTGAGGAAAACGTGAAATG+AGG | 0.583631 | 8.2:+7540321 | None:intergenic |
| TGGAAGCAGAAAGTATATGC+AGG | 0.591339 | 8.2:+7541411 | None:intergenic |
| TTCGGAGGCACCGTGTATTG+CGG | 0.594043 | 8.2:+7540509 | None:intergenic |
| AGCGAGCAGCAACATTACTC+AGG | 0.596950 | 8.2:+7540269 | None:intergenic |
| CTGCAACCTAATCCAGAAGG+TGG | 0.603070 | 8.2:-7541602 | MS.gene25643:CDS |
| GTGAAGGGTTCAGAAAGAGA+TGG | 0.604440 | 8.2:-7540425 | MS.gene25643:CDS |
| AAATCCTGGAACTGAAAACC+AGG | 0.610826 | 8.2:+7540365 | None:intergenic |
| GGATCAGACACCGCAATACA+CGG | 0.615581 | 8.2:-7540519 | MS.gene25643:CDS |
| AGGCGAGACATGGTACCAGA+TGG | 0.617797 | 8.2:+7541391 | None:intergenic |
| AATATGATGAAGGCGAGACA+TGG | 0.618034 | 8.2:+7541381 | None:intergenic |
| GAGACAGCAAAATCACTGGT+CGG | 0.647906 | 8.2:+7540467 | None:intergenic |
| ATATGCAGGTCGACACTGGT+CGG | 0.655426 | 8.2:+7541425 | None:intergenic |
| AATCTGCAACCTAATCCAGA+AGG | 0.663350 | 8.2:-7541605 | MS.gene25643:CDS |
| AAGTAGAAAGTTGAACAGAG+TGG | 0.677760 | 8.2:+7541555 | None:intergenic |
| GAACCAAACCAGACATTCGG+AGG | 0.677908 | 8.2:+7540494 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAGAAAAAAAAAAACAA+AGG | + | chr8.2:7540620-7540639 | None:intergenic | 10.0% |
| !! | TAAAATATGATTGATAAATA+AGG | + | chr8.2:7540913-7540932 | None:intergenic | 10.0% |
| !! | AAAAAAAAACAAAGGAATTA+CGG | + | chr8.2:7540612-7540631 | None:intergenic | 15.0% |
| !! | ATTACAAGATTAATTATATG+TGG | - | chr8.2:7540669-7540688 | MS.gene25643:intron | 15.0% |
| !! | TTTGTTAGTATAATAATACT+TGG | - | chr8.2:7541300-7541319 | MS.gene25643:intron | 15.0% |
| !! | AGATTGTTAATTCAGTATAT+GGG | - | chr8.2:7540761-7540780 | MS.gene25643:intron | 20.0% |
| !! | TAGATTGTTAATTCAGTATA+TGG | - | chr8.2:7540760-7540779 | MS.gene25643:intron | 20.0% |
| !!! | ATTTGATTTTACTTACATTC+AGG | + | chr8.2:7540406-7540425 | None:intergenic | 20.0% |
| !!! | CATTAGATTTGAAACATATT+GGG | - | chr8.2:7541009-7541028 | MS.gene25643:intron | 20.0% |
| !!! | TCATTAGATTTGAAACATAT+TGG | - | chr8.2:7541008-7541027 | MS.gene25643:intron | 20.0% |
| ! | AGTGTTAAATTTACATGTCT+TGG | - | chr8.2:7541367-7541386 | MS.gene25643:CDS | 25.0% |
| ! | TGATGAAATTGAAAAGAAAG+AGG | + | chr8.2:7540435-7540454 | None:intergenic | 25.0% |
| !!! | GGGGTTTTGAAATTTAATTT+TGG | - | chr8.2:7541036-7541055 | MS.gene25643:intron | 25.0% |
| GTTGAATTAACCAAAAACTG+TGG | + | chr8.2:7541174-7541193 | None:intergenic | 30.0% | |
| TTTGCATGTGTTGGAAAATT+GGG | - | chr8.2:7541262-7541281 | MS.gene25643:intron | 30.0% | |
| ! | ATTTGAAACATATTGGGAGT+TGG | - | chr8.2:7541015-7541034 | MS.gene25643:intron | 30.0% |
| ! | TGATTTTACTTACATTCAGG+TGG | + | chr8.2:7540403-7540422 | None:intergenic | 30.0% |
| ! | TTTGAAACATATTGGGAGTT+GGG | - | chr8.2:7541016-7541035 | MS.gene25643:intron | 30.0% |
| ! | TTTTGCATGTGTTGGAAAAT+TGG | - | chr8.2:7541261-7541280 | MS.gene25643:intron | 30.0% |
| !! | ATATTCCTTCTTCTGAAACA+TGG | - | chr8.2:7540561-7540580 | MS.gene25643:CDS | 30.0% |
| !!! | ATTTGTTTTATTGTAGGTGG+TGG | - | chr8.2:7541324-7541343 | MS.gene25643:intron | 30.0% |
| !!! | CTTGGCATTTGTTTTATTGT+AGG | - | chr8.2:7541318-7541337 | MS.gene25643:intron | 30.0% |
| AAAGAACCAAACCAGACATT+CGG | + | chr8.2:7541436-7541455 | None:intergenic | 35.0% | |
| AACAAAGGAATTACGGTGAT+AGG | + | chr8.2:7540605-7540624 | None:intergenic | 35.0% | |
| AAGTAGAAAGTTGAACAGAG+TGG | + | chr8.2:7540372-7540391 | None:intergenic | 35.0% | |
| AGCAATACACTTTGTAGCAA+CGG | + | chr8.2:7540838-7540857 | None:intergenic | 35.0% | |
| ATATACTTTCTGCTTCCATC+TGG | - | chr8.2:7540518-7540537 | MS.gene25643:CDS | 35.0% | |
| ATTGAGGAAAACGTGAAATG+AGG | + | chr8.2:7541606-7541625 | None:intergenic | 35.0% | |
| CAGAAGAAGGAATATGATGA+AGG | + | chr8.2:7540556-7540575 | None:intergenic | 35.0% | |
| CGAAAAGATTTGAGCAAACA+TGG | - | chr8.2:7541059-7541078 | MS.gene25643:intron | 35.0% | |
| GAAACATGGCATCATTACAT+TGG | - | chr8.2:7540575-7540594 | MS.gene25643:CDS | 35.0% | |
| GAAACTCATTACTCTCTTGT+AGG | - | chr8.2:7541523-7541542 | MS.gene25643:intron | 35.0% | |
| GGAACTGTATTAGACGATTT+CGG | + | chr8.2:7541085-7541104 | None:intergenic | 35.0% | |
| GTTCTTTAATGTGATCAACC+AGG | + | chr8.2:7540718-7540737 | None:intergenic | 35.0% | |
| GTTTAGCAAGTTCAAAATCC+TGG | + | chr8.2:7541576-7541595 | None:intergenic | 35.0% | |
| TAAAATCCACCTTCTGGATT+AGG | + | chr8.2:7540331-7540350 | None:intergenic | 35.0% | |
| TCTTGGATCTGATCTAACTA+AGG | - | chr8.2:7541384-7541403 | MS.gene25643:CDS | 35.0% | |
| TTCCAACACATGCAAAATTG+TGG | + | chr8.2:7541258-7541277 | None:intergenic | 35.0% | |
| TTGAAACATATTGGGAGTTG+GGG | - | chr8.2:7541017-7541036 | MS.gene25643:intron | 35.0% | |
| TTTACTTACATTCAGGTGGA+AGG | + | chr8.2:7540399-7540418 | None:intergenic | 35.0% | |
| TTTGCTCCGTAAATACTGAT+TGG | - | chr8.2:7540934-7540953 | MS.gene25643:intron | 35.0% | |
| ! | CATTTCACGTTTTCCTCAAT+TGG | - | chr8.2:7541606-7541625 | MS.gene25643:CDS | 35.0% |
| ! | TTGCTCCGTAAATACTGATT+GGG | - | chr8.2:7540935-7540954 | MS.gene25643:intron | 35.0% |
| !!! | GGCATTTGTTTTATTGTAGG+TGG | - | chr8.2:7541321-7541340 | MS.gene25643:intron | 35.0% |
| AAACTAACTCACTACGCAAG+AGG | + | chr8.2:7540861-7540880 | None:intergenic | 40.0% | |
| AAATCCTGGAACTGAAAACC+AGG | + | chr8.2:7541562-7541581 | None:intergenic | 40.0% | |
| AATATGATGAAGGCGAGACA+TGG | + | chr8.2:7540546-7540565 | None:intergenic | 40.0% | |
| AATCTGCAACCTAATCCAGA+AGG | - | chr8.2:7540319-7540338 | MS.gene25643:CDS | 40.0% | |
| ATCACATCTATAGCTGCATC+AGG | + | chr8.2:7541106-7541125 | None:intergenic | 40.0% | |
| CACCTCCCAATCAGTATTTA+CGG | + | chr8.2:7540943-7540962 | None:intergenic | 40.0% | |
| GATTGCAATAGTTGCGATTG+TGG | - | chr8.2:7540806-7540825 | MS.gene25643:intron | 40.0% | |
| TGATGCCATGTTTCAGAAGA+AGG | + | chr8.2:7540569-7540588 | None:intergenic | 40.0% | |
| ! | AGGTGTCGCAAGACAAAAAT+GGG | + | chr8.2:7540698-7540717 | None:intergenic | 40.0% |
| ! | GACCACAATTTTGCATGTGT+TGG | - | chr8.2:7541253-7541272 | MS.gene25643:intron | 40.0% |
| ! | TGGAAGCAGAAAGTATATGC+AGG | + | chr8.2:7540516-7540535 | None:intergenic | 40.0% |
| !! | ATTTTGCTGTCTCTGCAGAT+GGG | - | chr8.2:7541467-7541486 | MS.gene25643:intron | 40.0% |
| AAGTATATGCAGGTCGACAC+TGG | + | chr8.2:7540506-7540525 | None:intergenic | 45.0% | |
| GAGACAGCAAAATCACTGGT+CGG | + | chr8.2:7541460-7541479 | None:intergenic | 45.0% | |
| GATTGAAGATCACACTGCAG+TGG | - | chr8.2:7541123-7541142 | MS.gene25643:intron | 45.0% | |
| GTGAAGGGTTCAGAAAGAGA+TGG | - | chr8.2:7541499-7541518 | MS.gene25643:intron | 45.0% | |
| GTGACAAGAGACTCCAATTG+AGG | + | chr8.2:7541622-7541641 | None:intergenic | 45.0% | |
| TCCACGTAAAATCCACCTTC+TGG | + | chr8.2:7540337-7540356 | None:intergenic | 45.0% | |
| TGCAGAGACAGCAAAATCAC+TGG | + | chr8.2:7541464-7541483 | None:intergenic | 45.0% | |
| ! | CAGGTGTCGCAAGACAAAAA+TGG | + | chr8.2:7540699-7540718 | None:intergenic | 45.0% |
| ! | CATTTTTGTCTTGCGACACC+TGG | - | chr8.2:7540697-7540716 | MS.gene25643:intron | 45.0% |
| ! | CTCCGTAAATACTGATTGGG+AGG | - | chr8.2:7540938-7540957 | MS.gene25643:intron | 45.0% |
| !! | GATTTTGCTGTCTCTGCAGA+TGG | - | chr8.2:7541466-7541485 | MS.gene25643:intron | 45.0% |
| !! | TCCAGAAGGTGGATTTTACG+TGG | - | chr8.2:7540333-7540352 | MS.gene25643:CDS | 45.0% |
| !! | TTTTGCTGTCTCTGCAGATG+GGG | - | chr8.2:7541468-7541487 | MS.gene25643:intron | 45.0% |
| AGATGGGGGGTCATTTGTGA+AGG | - | chr8.2:7541483-7541502 | MS.gene25643:intron | 50.0% | |
| ATATGCAGGTCGACACTGGT+CGG | + | chr8.2:7540502-7540521 | None:intergenic | 50.0% | |
| CTGCAACCTAATCCAGAAGG+TGG | - | chr8.2:7540322-7540341 | MS.gene25643:CDS | 50.0% | |
| GAACCAAACCAGACATTCGG+AGG | + | chr8.2:7541433-7541452 | None:intergenic | 50.0% | |
| GATGGGGGGTCATTTGTGAA+GGG | - | chr8.2:7541484-7541503 | MS.gene25643:intron | 50.0% | |
| GGATCAGACACCGCAATACA+CGG | - | chr8.2:7541405-7541424 | MS.gene25643:CDS | 50.0% | |
| TGAAGATCACACTGCAGTGG+CGG | - | chr8.2:7541126-7541145 | MS.gene25643:intron | 50.0% | |
| ! | GCAGAGGCTACCACAGTTTT+TGG | - | chr8.2:7541161-7541180 | MS.gene25643:intron | 50.0% |
| ! | TTTGCTGTCTCTGCAGATGG+GGG | - | chr8.2:7541469-7541488 | MS.gene25643:intron | 50.0% |
| !! | TGCACCTGGTTTTCAGTTCC+AGG | - | chr8.2:7541555-7541574 | MS.gene25643:CDS | 50.0% |
| AGGCGAGACATGGTACCAGA+TGG | + | chr8.2:7540536-7540555 | None:intergenic | 55.0% | |
| GTAGGATGCACTTGTGCACC+TGG | - | chr8.2:7541541-7541560 | MS.gene25643:CDS | 55.0% | |
| GTGCCTCCGAATGTCTGGTT+TGG | - | chr8.2:7541427-7541446 | MS.gene25643:CDS | 55.0% | |
| TTGCTGTCTCTGCAGATGGG+GGG | - | chr8.2:7541470-7541489 | MS.gene25643:intron | 55.0% | |
| !! | TTCGGAGGCACCGTGTATTG+CGG | + | chr8.2:7541418-7541437 | None:intergenic | 55.0% |
| ACACGGTGCCTCCGAATGTC+TGG | - | chr8.2:7541422-7541441 | MS.gene25643:CDS | 60.0% | |
| GCGGTGCAATGCTGTTGCAG+AGG | - | chr8.2:7541145-7541164 | MS.gene25643:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 7540283 | 7541663 | 7540283 | ID=MS.gene25643 |
| chr8.2 | mRNA | 7540283 | 7541663 | 7540283 | ID=MS.gene25643.t1;Parent=MS.gene25643 |
| chr8.2 | exon | 7541537 | 7541663 | 7541537 | ID=MS.gene25643.t1.exon1;Parent=MS.gene25643.t1 |
| chr8.2 | CDS | 7541537 | 7541663 | 7541537 | ID=cds.MS.gene25643.t1;Parent=MS.gene25643.t1 |
| chr8.2 | exon | 7541336 | 7541457 | 7541336 | ID=MS.gene25643.t1.exon2;Parent=MS.gene25643.t1 |
| chr8.2 | CDS | 7541336 | 7541457 | 7541336 | ID=cds.MS.gene25643.t1;Parent=MS.gene25643.t1 |
| chr8.2 | exon | 7540283 | 7540606 | 7540283 | ID=MS.gene25643.t1.exon3;Parent=MS.gene25643.t1 |
| chr8.2 | CDS | 7540283 | 7540606 | 7540283 | ID=cds.MS.gene25643.t1;Parent=MS.gene25643.t1 |
| Gene Sequence |
| Protein sequence |
